AND THEIR USE IN TREATING TUMOUR CELLS

Technical Field The present invention relates to () -substituted guanine derivatives, a process for their preparation and their use in treating tumour cells. In particular, it relates to guanine derivatives having hetarylalkyl or naphthylalkyl substituents in the 0. position, these compounds exhibiting the ability to deplete 0 -alkylguanine-DNA alkyltransferase (ATase) activity in tumour cells.

Background Art

It has been suggested to use 0. -alkyl guanine derivatives possessing £ -alkylguanine-DNA alkyltransferase depleting activity in order to enhance the effectiveness of chemotherapeutic alkylating agents used for killing tumour cells. There is increasing evidence that in mammalian cells the toxic and utagenic effects of alkylating agents are to a large extent a consequence of alkylation at the 0 -position of guanine in DNA. The repair of 0. -alkylguanine is mediated by ATase, a repair protein that acts on the 0. -alkylated guanine residues by stoichiometric transfer of the alkyl group to a cysteine residue at the active site of the repair protein in an autoinactivating process. The importance of ATase in protecting cells against the biological effects of alkylating agents

has been most clearly demonstrated by the transfer and expression of cloned ATase genes or cDNAs into ATase deficient cells: this confers resistance to a variety of agents, principally those that methylate or chloroethylate DNA. Whilst the mechanism of cell killing by ()^-methylguanine in ATase deficient cells is not yet clear, killing by £ ⁶ -chloroethylguanine occurs through DNA interstrand crosslink formation to a cytosine residue on the opposite strand via a cyclic ethanoguanine intermediate, a process that is prevented by ATase-mediated chloroethyl group removal or complex formation.

The use of 0. - ethylguanine and 0. -ji-butylguanine for depleting ATase activity has been investigated (Dolan et. aj_. , Cancer Res. , (1986) 46, pp. 4500; Dolan et a - 1 Cancer Chemother. Pharmacol. , (1989) 25, pp 103. 0 -benzylguanine derivatives have been proposed for depleting ATase activity in order to render ATase expressing cells more susceptible to the cytotoxic effects of chloroethylating agents (Moschel et al. , J. ed.Chem.. 1992, 35 , 4486). U.S. Patent 5 091 430 and International Patent Application No. W091/13898 Moschel et ah disclose a method for depleting levels of ^-alkylguanine-DNA alkyl-transferase in tumour cells in a host which comprises administering to the host an effective amount of a composition containing 0. -benzylated guanine derivatives of the following formula:

wherein Z is hydrogen, or

and R ^a is a benzyl group or a substituted benzyl group. A benzyl group may be substituted at the ortho, meta or para position with a

substituent group such as halogen, nitro, aryl such as phenyl or substituted phenyl, alkyl of 1-4 carbon atoms, alkoxy of 1-4 carbon atoms, alkenyl of up to 4 carbon atoms, alkynyl of up to 4 carbon atoms, amino, monoalkyla ino, dialkylamino, trifluoromethyl , hydroxy, hydroxymethyl , and S0 _n R wherein n is 0, 1, 2 or 3 and R is hydrogen, alkyl of 1-4 carbon atoms or aryl. Mi-Young Chae et a±. , J.Med.Chem., 1994, 37, 342-347 - published after the priority date of the present application - describes tests on CJ -benzylguanine analogs bearing increasingly bulky substituent groups on the benzene ring or at position 9. Compound No. 6 described therein is r (2-pyridylmethyl)guanine, which in this application is called 0 ⁶ -(2-picolyl) guanine. However in the Results and Discussion at pages 342 - 343 of the Chae et. a . paper, Compound No. 6 is not highlighted as being of interest but is grouped among "remaining compounds" which "exhibited intermediate activity" (Page 343 Lines 12 - 15 of the text). The authors confirm their earlier observations (J.Med.Chem.. 1992, 35 4486) that only allyl or benzyl substituents at the () position of guanine efficiently inactivated ATase (page

343 lines 21 - 23 of the text).

() -benzylguanine has limitations in its use as an ATase inactivator. It is more stable than would be desirable, resulting in a long survival time in an animal to which it is administered. It has a level of potential toxicity both alone and in combination with chloroethylating agents which is also undesirable and which may be related to the survival time.

The compounds of the present application exhibit different ATase inactivating characteristics from 0 -benzylguanine and in some cases the activity is up to 8 times greater than that of

0. -benzylguanine. Different half-life and toxicity characteristics have also been observed. Therefore, it is an object of the present invention to provide novel compounds useful for depleting ATase activity in order to enhance the effects of chemotherapeutic agents such as chloroethylating or methylating anti-tumour agents.

Another object of the invention is to provide pharmaceutical compositions containing compounds which are useful for depleting ATase activity. A further object of the present invention is to provide a

method for depleting ATase activity in tumour cells. A still further object of the invention is to provide a method for treating tumour cells in a host.

Disclosure of Invention

Accordingly, the present invention provides .0 -substituted guanine derivatives of formula I:

wherein

Y is H, ribosyl, deoxyribosyl , or R"XCHR"', wherein X is 0 or S, R" and R"' are alkyl, or substituted derivatives thereof;

R' is H, alkyl or hydroxyalkyl;

R is (i) a cyclic group having at least one 5- or 6-membered heterocyclic ring, optionally with a carbocyclic or heterocyclic ring fused thereto, the or each heterocyclic ring having at least one hetero atom chosen from 0, N, or S, or a substituted derivative thereof; or

(ii) naphthyl or a substituted derivative thereof;

and pharmaceutically acceptable salts thereof.

R may suitably be a 5- or 6-membered heterocyclic ring or a benzo derivative thereof, in which latter case the 0 -alkyl guanine moiety may be attached to R at either the heterocyclic or the benzene ring.

in preferred embodiments, R is a 5-membered ring containing S or 0, with or without a second ring fused thereto.

Preferably, R is a heterocyclic ring having at least one S atom; more preferably, R is a 5-membered heterocyclic ring having at least one

S atom; and most preferably, R is a thiophene ring or a substituted derivative thereof.

Alternatively, R may be a heterocyclic ring having at least one 0 atom, particularly, a 5-membered heterocyclic ring having at least one 0 atom and more particularly R may be a furan ring or a substituted derivative thereof.

As another alternative, R may be a heterocyclic ring having at least one N atom, particularly R may be a 6-membered heterocyclic ring having at least one N atom and in particular, R may be a pyridine ring.In the definition of Y, the term "substituted derivative" includes substitution by one or more of the following groups: hydroxy, alkoxy, amino, alkylamino, amido or ureido.

In the definition of R, the term "substituted derivative" includes substitution of the heterocyclic rings and/or carbocyclic ring(s) by one or more of the following groups: alkyl, alkenyl, alkynyl, halo, haloalkyl, nitro, cyano, hydroxyalkyl, SO _n R"" where R'' ¹ ' is alkyl and n= 0,1 or 2, or a carboxyl or ester group of the formula -C00R wherein R is H or alkyl. Halo, haloalkyl, cyano,

5 5 SO R ¹ '' ¹ (as defined above) and -C00R wherein R is alkyl are preferred substituents.

An alkyl, alkenyl, or alkynyl group preferably contains from 1 to 20, more preferably from 1 to 10 and most preferably from 1 to 5 carbon atoms. Halo includes iodo, bro o, chloro or fluoro .

Examples of compounds of the invention (together with Compounds B.4214 and B.4218 not covered by the present application) are shown in Table 1.

TABLE 1

B.4203 £ -furfurylguanine

B.4205 £ -thenylguanine

B.4206 £ -(3-thienylmethyl)guanine

B.4209 £ -(3-f urylmethyl )guanine

B.4210 £ ⁶ -(2-picolyl)guanine

B.4211 £ -( 3-pi co lyl ) guan ine

B.4212 £ -piperonylguanine

B.4213 £ -(2-naphthylmethyl )guanine

B.4214 DL-£o-(Cf -methylbenzyl)guanine

B.4217 DL-£ -(C( -methylthenyl)guanine

B.4218 £ -(2-methylbenzyl)guaniπe

B.4219 DL-£ ^υ -[l-(3-thienyl)ethyl]guanine

B.4220 £ -(5-methylthenyl)guanine

B.4221 £ -(5-methylfurfuryl)guanine

B.4222 £ -(3-methylthenyl) guanine // \\ ₀ G

B.4226 £ -(2-benzo[b]thienylmethyl)guanine

B.4229

B.4234 £ -(5-carboxyfurfuryl) guanine

B.4265 £ -(l-naphthyl ethyl)guanine

B.4266 £ -(2-benzof uranylmethyl )guam ^" ne

B.4268 £ -piperonylguanosine

OH OH

B.4269 £ -(5-bromothenyl)guanine Br yyOG

B.4271 £ -(5-azapiperonyl)guanine

B.4273 £ -(5-cyanofurfuryl)guanine // \\ _Q Q

B.4274 £ -(5-oxazolylmethyl)guanine

B.4275

B.4276 0 ,t° ⁾ -(2-benzo[b]thienylmethyl

B.4277 0 ^b -(4-picolyl)guanine

B.4278 £ ⁶ (l-methyl-4-nitropyrrol-2-ylmethyl ) guanine

I CH.

B.4279 £°-thenyl guanos ine

Among the compounds in Table 1 compounds B.4214 and B.4218 are not compounds of the invention. Compound B.4210 (ie the compound of Formula I in which R is 2-pyridyl, R' is H and Y is H) is not a preferred compound of the invention. Particularly preferred compounds of the invention include: B.4205 £ -thenylguanine B.4206 £ -(3-thienylmethyl )guanine B.4212 £ -piperonylguanine B.4226 £ -(2-benzo[b]thienylmethyl )guanine B.4266 £ -(2-benzofuranylmethyl)guanine B.4275 £ -(5-thiazolylmethyl)guanine and compounds substituted in the heterocyclic ring of R by a halo, cyano or ester group, including

B.4229 £ -(5-methoxycarbonylfurfuryl )guanine B.4269 £ -(5-bromothenyl)guanine

B.4273 £ -(5-cyanofurfuryl)guanine. Other preferred compounds include B.4209 £ -3-furylmethylguanine B.4276 £ -(2-benzo[b]thienylmethyl)guanosine B.4277 £ (4-picolyl)guanine.

The most preferred compounds of the invention are those that inactivate ATase in vitro and/or in mammalian cells and/or tumour xenografts more effectively than £ -benzylguanine (BeG) and that sensitise mammalian cells and/or tumour xenografts to the killing or growth inhibitory effects of nitrosoureas and or methylating agents more effectively than BeG. Preferred compounds should also have, in comparison to BeG, reduced toxicity to normal tissues and/or to the entire organism when used in combination with such agents. Preferred compounds should not themselves be toxic or show more than minimal toxicity at the doses required to inactivate ATase, neither should any hydrolysis products of a preferred compound that was chemically unstable be toxic. Although the invention is not limited by any theory, preferred compounds may need to be less stable than BeG so that they undergo spontaneous chemical degradation soon after achieving maximal inactivation of ATase: in this way any action of metabolic processes that might act on the agent to generate toxic species would be minimised. Preferred compounds should be less able to sensitise human bone marrow or other normal cell types to the toxic

effects of alkylating agents so that they would not exacerbate the known toxicity or generate new toxicities of these agents in normal human tissues.

Preferred compounds of the invention include those having a relatively low I™ value in Table 4 herein (e.g. below l.OμM, more particularly below 0.04uM) and/or having a relatively short half life in Buffer I (representing conditions for an j_n vitro assay) and/or Phosphate buffered saline (PBS) (representing conditions in physiological medium) in Table 4 herein (e.g. below 20 hours in Buffer I or below 16 hours in PBS).

A relatively short half-life can be regarded as an indicator that a compound of the invention would be less stable than £^-benzylguanine due to the reactivity of RR'CH- and would tend to break down by hydrolysis in physiological medium.

The influence of the group RR'CH- in the compounds of formula I enabling them to act as ATase inhibitors is determined by electronic, steric and physicochemical factors. Steric factors may be related to the nature of the environment of the cysteine receptor site in ATase. Preferably R ¹ is H. A secondary carbon atom attached to £ (as in the DL compounds B.4214 or B.4217) has been found to reduce greatly the inactivating activity, probably because of the bulk of the substituent.

Preferably the cyclic group in R does not have a methyl group in the vicinal position (as in B.4222) although the influence of vicinal substitution is evidently much less when R is heterocyclic than in the naphthyl isomers B.4213 and B.4265.

Physicochemical factors such as stability, solubility and water-!ipid partition are relevant to the selection of compounds for use in vivo, affecting formulation, absorption and transport, for example. Selection of compounds may also be influenced by their differential distribution into different tissues.

One embodiment of the invention provides a pharmaceutical composition containing compounds of formula 1, wherein Y, R and R ¹ are

as defined above, or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable excipient. Optionally the composition may also contain an alkylating agent such as a chloroethylating or methylating agent.

In a further embodiment, the present invention provides a method for depleting ATase activity in a host comprising administering to the host an effective amount of a composition containing a compound of formula 1 wherein Y, R and R ¹ are as defined above, or a pharmaceutically acceptable salt thereof, more particularly a pharmaceutical composition as defined above. This method may alternatively be defined as a method of depleting ATase mediated DNA repair activity in a host.

The invention further provides a method for treating tumour cells in a host comprising administering to the host an effective amount of a composition containing a compound of formula I wherein Y, R and R ¹ are as defined above or a pharmaceutically acceptable salt thereof, more particularly a pharmaceutical composition as defined above and administering to the host an effective amount of a composition containing an alkylating agent. The method may be used for treatment of neoplasms including those which are. known to be sensitive to the action of alkylating agents e.g. melanoma and glioma and others whose resistance to treatment with alkylating agents alone may be overcome by the use of an inactivator according to the invention.

The invention also provides a process for preparing compounds of formula I comprising the steps of: reacting sodium hydride with a solution of RR'CHOH (wherein R and R' are as defined above) in an organic solvent, preferably at or below room temperature; adding 2-amino-N.,N.,N.-trimethyl-lH.-purin-6-aminium chloride or 2-amino-6-chloropurine riboside; treating the reaction mixture with weak acid and ether; and extracting the desired product.

Brief Description of Drawings

The invention shall be described in greater detail with reference to the accompanying drawings in which:

Figure 1 is a graph of percentage residual activity of purified recombinant human ATase following incubation with different concentrations of various inactivators. Each point shows the mean of 3 measurements. The line at 50% residual activity is used for calculating I _™ values i.e. the concentration of inactivator required to produce a 50% reduction in ATase activity.

Figure 2 is two graphs of percentage cell growth against alkylating agent concentration (μg/ l), showing the sensitization effect of £ -benzylguanine (BeG) and £ -thenylguanine (B.4205) at two different concentrations (0.1 and l.OμM) on sensitization of Raji cells to BCNU. The line at 90% growth is used for calculating D _go values i.e. the dose of BCNU at which there was 90% growth as compared to untreated controls i.e. 10% growth inhibition.

Figure 3 is four graphs of percentages cell growth, against alkylating agent concentration ( M), showing the effect of BeG and B.4205 at four different concentrations (0.1, 0.5,1.0 and 5.0iM) on sensitization of Raji cells to temozolomide.

Figure 4 is a graph extrapolated from Figure 3 of values for ^D (μM) (i.e. the dose of temozolomide at which there was 50% growth as compared to untreated controls) against inactivator concentration (μM).

Figure 5 is two graphs of percentage cell growth against inactivator concentration, showing the growth inhibiting effect of £ ⁶ -furfurylguanine (B.4203) and B.4205 on Chinese hamster V79 cells(RJKO) and a subline thereof (+120).

Figure 6 is two graphs of percentage cell growth against inactivator concentration, showing the growth inhibition effect of B.4203 and B.4205 on two Xeroderma piqmentosum subclones.

Figure 7 is four graphs of percentage cell growth against inactivator concentration (μM) showing the effect of degradation products of inactivators B.4203, B.4205, B.4212 (£ -piperonylguanine) and B.4226 (£ ⁶ -[2-benzo(b)thienylmethyl)guanine) on Raji cell growth.

Figure 8 is a diagram of ATase activity (fm/mg) against time (hours) showing the depletion of ATase activity in A375 xenografts in nude mice for untreated controls, corn oil treated controls and BeG (30 mg/kg) and B.4205 (30 mg/kg) treated extracts.

Figures 9 - 13 are graphs of results of xenograft studies. In each figure the top graph shows percentage tumour volume against time (days) for A375 tumour xenografts in nude mice treated with BCNU alone, with BeG in combination with BCNU, and B.4205 in combination with BCNU. The lower graph in each figure shows the number of surviving mice in each treatment group against time (days) following the treatments illustrated in the top graph.

The particulars for each figure are as follows:- Figure Mice BCNU Concentration Inactivator

(mg/kg) (mg/kg)

9 ASU Approx. 15 60

10 ASU 25 60

11 0LAC 10 30

12 ASU 16 60

13 ASU 16 60

Figure 14 is 3 graphs of percentage survival against temozolomide concentration (jiM), showing the survival of bone marrow cells following treatment with inactivator (10/ιM) or DMSO (control) in combination with increasing doses of temozolomide.

Modes for carrying out the Invention

£ -hetarylalkylguanine derivatives having ATase inactivating characteristics may be synthesized by adapting the standard preparation presented below as appropriate.

2-Amino-ϋ,N.,N.-trimethyl-lH-purin-6-aminium chloride is prepared in accordance with the procedure described by Kiburis et. a , J_ _; _

Chem.Soc. (C), 1971, 3942. Details of the conditions for reaction of this quaternary salt with sodium benzyloxide (to give £ -benzyl-guanine) not disclosed in MacCoss, Chen and Tolman, Tetrahedron Lett. , 1985, 26, 1815, were given in MacCoss, Tolman, Wagner and Hannah, European Patent Application No. 184,473, but these were not suitable for preparation of relatively sensitive analogues, and the standard preparation below was devised by the inventors.

Standard preparation of 0 -hetarylalkylguanines (Formula I, y = H) Sodium hydride (60% in oil; 0.8 g, 20 mmol) is added to a solution of RR'CHOH (56 tnrnol, ca. 5 ml) in DMSO (5 ml) and the mixture is stirred at room temperature for 1 hour. For solid or higher molecular weight alcohols, up to 10ml DMSO may be used instead of 5ml. 2-Amino-N.,N.,N.-trimethyl-lH.- purin-6-aminium chloride (2.29 g, 10 mmol) is added and stirring is continued for a further 1 hour. The change in UV spectrum is then complete (X _maχ 312— ^■ 284 nm) and the almost clear solution is treated with acetic acid (1.7 ml). After cooling and dilution with ether (300 ml), the mixture is set aside (2 hours) and the solid (A) collected. Trituration with water gives the product. A second fraction can be obtained by evaporation of the ether-DMSO filtrate and trituration of the residue successively with ether and water. Alternatively the product may be extracted from the solid (A) with warm acetonitrile. The recrystallised compounds show a single spot in TLC (CgHg-MeOH, 4:1) and are characterised by analysis and their NMR spectra. Frequently, they contain solvent of crystallisation. Melting points and analytical data are given in Table 2, UV and H NMR data in Table 3. NMR spectra were measured on a Bruker WP80 or MSL 300 instruments.

This standard preparation procedure (with variations indicated by symbols in Table 2) was used to make the compounds listed in Tables 2a and 3a. In compounds B.4217 and B.4219, R' is methyl; in the remaining compounds R' is H. £ -benzylguanine and Compounds B4214, B4218 and B4231 listed in Table 4 below were also made by this standard preparation procedure for comparative testing purposes.

The variations indicated by the symbols in Table 2 are as follows: a. 5 mmol sodium hydride per mmol quaternary salt are used in preparation of this compound. When the standard amount (2 mmol)

is used, 40% of the quaternary salt can be recovered in the reaction work-up.

b This compound is made by hydrolysis of the methyl ester B.4229 (145 mg, 0.5 mmol) in 2-methoxyethanol (2.5 ml) and water (2.5 ml) by treatment with 2M-NaOH (2.5 ml) for 4 hours at room temperature. Neutralisation with acetic acid (0.32 ml, 5.5 mmol), gentle evaporation, trituration with water (3 ml) and filtration give a solid which on extraction with hot methanol yields the acid B.4234.

c The required figures are based on the monohydrate of a mixture of 4 parts of sodium salt of the acid and 3 parts of the acid, requiring Na, 4.3%. Found: Na, 4.44%.

3 mmol alcohol RCH ₂ 0H per mmol quaternary salt are used instead of the standard 5.6 mmol.

e For alcohols which are too sensitive to sodium hydride in DMSO at room temperature, RCH ₂ 0Na is prepared in DMF (2.5 ml at -10°C; 3 mmol RCH ₂ 0H; 2 mmol sodium hydride). 1 mmol quaternary salt is added after 15-20 minutes and stirring continued for 2-3 hours at room temperature

f. Products are extracted with acetonitrile.

Preparation of Ribosides (formula I, Y= ribosyl)

A solution of alkoxide made as in the Standard Procedure above from sodium hydride (60% in oil; 120 mg, 3mmol) and RR'CHOH (4.6 mmol) in dry DMSO (2 ml) during 1 hour is treated with

2-amino-6-chloropurine riboside (302 mg, 1 mmol) and stirred for 5 minutes at room temperature, then 15 minutes at 60-65 C. The reaction is then complete as indicated by the change in UV spectrum

( ~X 311—»284nm). Cooling and thorough trituration with ether /\max (100 + 15 ml) and filtration yield a solid which is treated with water

(10 ml). The pH is brought from 11 to 8 by passing C0 ₂ briefly.

Filtration removes inorganic material and the dried residue is extracted at room temperature with methanol (4 x 10 ml portions, each containing a drop of pyridine). Evaporation of almost all the

methanol and addition of a little ether yield the product, almost pure by TLC (C ₆ H _g -MeOH 4:1). It is recrystrallised from methanol and a trace of pyridine, dissolving and concentrating below 40°C, sometimes with final addition of a little ether.

This procedure was used to make the compounds listed in Tables 2b and 3b. Traces of impurities are difficult to remove but NMR spectra show that the nucleosides are .ca. 90% pure. Yields are of the order of 30-40%.

TABLE 2a

Test No. 0 ⁶ -Substituent RR ¹ CH- Analysis

B.4203 furfuryl

B.4205 t enyl

B.4206 3-thienylmethyl

B.4209 3-furylmethyl

B.4210 2-picolyl

B.4211 3-picolyl

B.4212 plperoπyl

B.4213 2-naphthylmethyl

B.4217 C(-methylthenyll 27 MeCN 150 Upwards

B.4219 1-(3-th!enyl)ethylt 57 MeCN 150 Upwards if lf ¹ ! OS Found 50.59 4.33 27.10

TABLE 2a continued

B.4226 2-benzo[b]thlenylmethyl 66 MeOH 198-208 C^H^NgOS.MeOH

B.4229 5-methoxycarbonyl 43 MeOH 120-130 (with ^C 12 ^N 11 ^N 5°4- ¹ - ^5H 2° -furfuryllti effervescence)

B.4234 5-carboxyfurfuryl 67 MeOH 210-260 CnH _{9 5} 04.H ₂ 0C

B.4265 1-naphthylmethyl 75 MeOH 210 Upwards C ₁₆ H ₁₃ N ₅ O.0.5MeOH

B.4266 2-benzofuranylmethyl 58 MeOH 196-198 C^H^ gO^ eOH

B.4269 δ-bromolhenyl^&i 14 EtOH 170-180 C ₁₀ H ₈ BrN ₅ OS

B.4271 5-azapiperonyld 63 EtOH 230-240 C ₁₂ H ₁₀ N ₆ O ₃ .0.25EtOH

TABLE 2a (continued)

Test No. 0 ⁶ -Substituent Yield % Solvent for M.p.(decomp) Formula RR ¹ CH- (based on recrystπ. ( °o solvate)

B.4273 5-cyanofurfurylϋ 15 MeOH 91-100 (with CnH ₈ N ₆ 0 ₂ .H2θ effervescence) B.4274 5-oxazolylmethylϋ 32 MeOH 180-215 C ₉ H ₈ N ₆ O ₂ .0.25H ₂ O

B.4275 5-t iazolylmethyl4! 40 MeOH 190-220 C ₉ H ₈ H ₆ OS.0.5H ₂ O

B.4277 4-plcolyώ 72 MeOH 230 Upwards CnH ₁₀ N ₆ O

B.4278 1-methyl-4-nltro- pyrrol-2-ylmethyliL. ^β 22 MeOH 140-210 ^c 11 11 ^N 7°3 ⁰ - ^5H 2 ^o

TABLE 2b

Test No. O ^β -Substituent Yield % Solvent for M.p.(deco p) Formula RR ¹ CH- (based on recrystn. ( °C) solvate)

Ribosldes

B.4268 piperonyl MeOH from 150 (with C ₁₈ H ₁₉ N ₅ 0 ₇ .2H ₂ 0 effervescence)

B.4276 2-benzotb]thienylmethyl MeOH- 140-155 C ₁₉ H _1g N ₅ 0 ₅ S.H ₂ 0 ether B.4279 thenyl MeOH- from 120 (with C ₁₅ H ₁₇ N ₅ O ₅ S.0.5H ₂ O ether effervescence)

S H [ppm from TMS;(CD ₃ ) ₂ SO], J (Hz)

5.43(s), 6.32(s), 6.49(dd,J 3.1,1.5), 6.66 (d,J 3.1), 7.71 (d,J 1.5),7.81 (s), 12.42(bs)

5.67(S), 6.30(s), 7.04(dd,J 5.1, 3.5),7.31 (dd, J 3.5,1.2), 7.56(dd, J 5.1, 1.2), 7.84(s), 12.47(bs)

5.47(s), 6.27(s), 7.26(dd, J 4.8, 1.2),7.54 (dd, J 4.8, 2.9), 7.62(bs), 7.80(s), 12.40(bs)

5.34(s), 6.26(3), 6.65(d, J 1.4), 7.66 (t, J 1.4), 7.82(s), 7.83(bs), 12.41 (bs)

5.58(s), 6.27(S), 7.50(m), 7.84(e), 8.58(dd, J 4.8,1.2), 12.47(bs)

5.58(s), 6.36(s),7.49(dd,J 7.9, 4.8),7.87(s), 8.01 (dt,J 7.9, 1.8), 8.62(dd, J 4.8, 1.8), 8.80(bs), 12.48(bs)

5.38(s), 6.02(s), 6.24(S), 6.95(m), 7.79(s), 12.37(bs)

5.67(s), 6.28(bs), 7.46-8.04(m), 12.47(bs)

1.74(d, J 6.6), 6.24(s), 6.74(q, J 6.6), 7.01 (m), 7.24(m), 7.49(m), 7.81 (s), 12.40(bs)

1.67(d, J 6.5), 6.20(s), 6.54(q, J 6.5), 7.24(m) 7.50 (m), 7.82(s), 12.37(bs)

2.42 (d, J 3.4), 5.57(S), 6.25(s), 6.70(d, J 3.4), 7.08(d), 7.81 (s), 12.41 (bs)

2.28(s), 5.37(s), 6.11 (m), 6.27(s), β.53(d, J 3.0), 7.81 (s), 12.44(bs) 2.28(s), 5.60(s), 6.25(3), 6.91 (d, J 5.0), 7.45 (d, J 5.0), 7.80(3), 12.44(bs)

&μ [ppm from TMS;(CD ₃ ) ₂ SO], J (Hz)

5.78(3), 6.38(s), 7.38(m), 7.60(s), 7.83(s), 7.85(m), 12.47(bs)

3.82(3), 5.50(S), 6.39(S), 6.87 (d, J 3.4),

5.43(8), β.35(s), 6.68(d, J 3.3), 6.87 (d, J 3.3),

5.96(3), 6.36(3), 7.58(m), 7.72(d, J 6.8) 7.79 (s) cπ 7.89(m), 8.11 (m), 12.44(bs)

5.62(3), 6.40(s), 7.13(3), 7.27 (dt, J 7.3, 1.1), 7.34(dt, J 7.3, 1.3), 7.65(m), 7.68 (m), 7.85 (s), 12.48 (bs)

5.62(3), 6.36(s), 7.17 (ABq, J 3.7) 7.85(s), 12.47(bs)

5.40(3), 6.18(S), 6.39(s), 7.48 (d, J 1.8), 7.83(3), 12.46(bs)

TABLE 3a (continued)

Test No. 0 ⁶ -Substituent

B.4273 5-cyanofurfuryl 248,286 5.53(8), 6.42(s), 6.99(d, J 3.5), 7.67(d, J 3.5)

(RCH ₂ OH: 248) 7.87(s), 12.52(bs)

B.4274 5-oxazolylmethyl 243,286 5.56(3), β.38(s), 7.44(s), 7.85(s), 8.45(s),

12.48(bs)

B.4275 5-thiazolylmethyl 244,286 5.76(3), β.42(s), 7.85(s), 8.14(s), 9.13(s),

12.49(bs)

B.4277 4-picolyl 244,2653h,286 5.58(s), 6.34(s), 7.47(d, J 5.7), 7.88(s),

8.60 (d, J 5.7), 12.51 (bs) ro

B.4278 1-methyl-4-nitro- 244,285,3203h 3.78(3), 5.46(3), 6.40(s), β.98(s), 7.84(s), as pyrrol-2-ylmethyl (RCH ₂ OH: 280, 8.08(s), 12.49(bs)

320)

TABLE 3b

Test No. 0 ⁶ -Substituent λ

Ribosides

B.4268 piperonyl 246,287 3.53 and 3.62(2 x dd, J 11.9, 3.75), 3.89 (q, J 3.5),

(RCH ₂ OH 4.10(dd, J 4.9, 3.5), 4.45 (t, J 5.5), 5.1 (bs),

240,288) 5.38(e), 5.78 (d, J 6.0), 6.02(s), 6.51 (s), 6.93(d, J 7.7), 7.00 (dd, J 7.7, 1.5), 7.10(d, J 1.5), 8.10(S)

B.4276 2-benzo[b]thienylmethyl 241,252, 3.57 and 3.67(2 x m), 3.91 (q, J 3.6), 4.12 (q, J 5.2), 269,287 5.16 (m), 5.45(d, J 6.2), 5.83 (s + m), 6.61(s), 7.40(m), 7.63(3), 7.88(m), 7.97(m), 8.16(3)

(RCH ₂ 0H 241,262 IV) 289w, 300w)

B.4279 thenyl 246,285 3.57 and 3.64 (2 x m), 3.92(q, J 3.5), 4.13(m),

4.49 (q, J 5.2), 5.16(m), 5.45(m), 5.70(s), 5.81 (d,J 6.0),

6.57(3), 7.07(dd, J 5.1, 3.5), 7.34 (d, J 0.9),

7.60(d, J 1.3), 8.14(s), 12.40(bs)

The starting alcohols RR'CHOH were usually made by reduction of the corresponding aldehydes, often commercially available, by sodium borohydride. A different approach was used for the precursors of the ester B.4229 and the nitrile B.4273. Sucrose was converted via 5-chloromethylfurfural into 5-hydroxymethylfurfural. Oxidation of this aldehyde gave the carboxylic acid which was esterfied by the method of Bocchi et al- to methyl 5-(hydroxymethyl)furoate, required for B.4229. The oxime of the aldehyde was dehydrated by the method of Carotti et. l. ; ^" the crude reaction product was treated with cone, aqueous ammonia in methanol before extraction into dichloromethane. Distillation afforded 5-cyanofurfuryl alcohol, required for B.4273.

For B.4266, Vilsmeier reaction of benzofuran yielded the g 2-aldehyde, reduced to the required alcohol, while for B.4226 lithiation and treatment with dimethylformamide gave the 2-aldehyde and in turn the alcohol.

For B.4274, dimethyl tartrate was oxidised to methyl

12 glyoxylate which reacted with tosyl ethyl isocyanide to give methvl oxazole-5-carboxylate. This was reduced by lithium

14 15 aluminium hydride by the method of Fallab to the alcohol.

For B.4275, bromomalonaldehyde and thiourea yielded

2-aminothiazole-5-carboxaldehyde. Deamination by amyl nitrite followed by sodium borohydride reduction gave

_ 14 5-hydroxymethylthiazole .

For B.4271, 5-azapiperonyl alcohol ( .p. 82-84°C; found, C, 54.60; H, 4.58; N, 9.09; C _? H ₇ N0 ₃ requires C, 54.90; H.4.61; N, 9.15%) was prepared from the corresponding aldehyde.

19 For B.4278, l-methylpyrrole-2-carboxaldehyde was nitrated and

20 reduced to the alcohol. by sodium borohydride.

By way of specific example, the preparation of CJ -thenylguanine (B.4205) will now be described:

Preparation of 0 -thenylguanine

A solution of thenyl alcohol (3.18 ml, 33.6 mmol) in DMSO (3 ml) was treated with sodium hydride (60% in oil; 0.48g, 12 mmol), stirring cautiously at first. After 1 hour 2-amino-N., H ,

N-trimethyl-lH.-purin-6-aminium chloride (1.37g, 6 mmol) was added and stirring continued 1 hour more. Acetic acid (1.0 ml) was added, cooling briefly, and the mixture diluted with ether (180 ml). The solid (2.09g) was collected after l-2h. Evaporation of ether from the filtrate and distillation of DMSO and excess thenyl alcohol (b.p. 48-57°C/0.2 mm) left a residue which on trituration with ether yielded a second solid fraction (0.36g). The combined solids were rubbed (trituration) with water (6 ml), yielding product (1.335g, 90%) showing a strong spot in TLC (CgHg-MeOH, 4:1) with only traces of impurity. Dissolution in hot ethanol (30 ml), clarification by filtration through Celite, and concentration (to 10 ml) using a rotary evaporator yielded B.4205 (1.125g, 71% of material containing 1/3 EtOH per mole as solvate).

Compounds of formula I in which Y is R''XCHR'" (seco-nucleosides) may be prepared by an analogous preparation to the reaction of 0. -benzylguanine with o -chloro-ethers (MacCoss et al.. Tetrahedron Lett.; European Patent Application No. 184,473., loc. cit.) or with alkyl bromides (e.g. Kjellberg, Liljenberg and Johansson, Tetrahedron Lett.. 1986, 27, 877; Moschel, McDougall , Dolan, Stine, and Pegg, J.Med. Chem., 1992, 35, 4486).

Typical "sugar" components corresponding to R^XCHR'' ¹ , leading to seco-nucleosides, are made by methods described in e^.. McCor ick and McElhinney, J. Chem. Soc. Perkin Trans. 1. 1985, 93; Lucey, McCormick and McElhinney, J. Chem. Soc, Perkin Trans. 1. 1990, 795.

Compounds of formula I in which Y is ribosyl or deoxyribosyl (nucleosides) may be prepared by methods analogous to the syntheses of fj ⁶ -benzylguanine riboside and 2-deoxyriboside (Moschel et. a . 1992; cf. Gao, Fathi, Gaffney et al.. J. Orq. Chem., 1992, 57, 6954; Moschel, Hudgins and Dipple, J. Amer. Chem. Soc. 1981, 103, 5489) (see preparation of Ribosides above).

Industrial Applicability

The amount of the compound of the present invention to be used

varies according to the effective amount required for treating tumour cells. A suitable dosage is that which will result in a concentration of the compound of the invention in the tumor cells to be treated which results in the depletion of ATase activity, e.g. about 1 - 2000 mg/kg, and preferably 1 - 800 mg/kg, particularly 1-120 mg/kg, prior to chemotherapy with an alkylating agent.

The pharmaceutical composition of the invention may be formulated in conventional forms with conventional excipients, as described for example in W0 91/13898, the contents of which are incorporated herein by reference. The composition may contain the inactivator according to the invention together with an alkylating agent; or the composition may comprise two parts, one containing the inactivator and the other containing the alkylating agent. The method of administering the compounds of the invention to a host may also be a conventional method, as described in W0 91/13898 for example. For administration of an inactivator according to the invention to patients, the pharmaceutical composition may suitably contain the inactivator in a suitable vehicle such as 40% polyethyleneglycol 400 in saline solution, or in saline or 3% ethanol (in saline), for intravenous injection, or in a powder form in suitable capsules for oral administration.

Alkylating agents may be administered in accordance with known techniques and in conventional forms of administration, as described in W0 91/13898 for example or preferably as a single dose immediately after or up to 24 hours after but preferably around 2 hours after administration of the ATase inactivating agents and also at doses lower than those used in standard treatment regimen. A reduction in dose may be necessary because the inactivators would generally be anticipated to increase the toxicity of the alkylating agents. Examples of chloroethylating agents include 1,3 bis (2-chloroethyl) -1-nitrosourea (BCNU) , l-(2-chloroethyl )-3-cyclohexyl-l-nitrosourea (CCNU), fotemustine, mitozolomide and clomesone and those described in McCormick, McElhinney, McMurry and Maxwell J. Chem. Soc. Perkin Trans. 1, 1991, 877 and Bibby, Double, McCormick, McElhinney, Radacic, Pratesi and Dumont Anti-Cancer Drug Design, 1993, 8, 115. Examples of methylating agents include temozolomide (British Patent GB 2 104 522) and dacarbazine, procarbazine, and streptozocin.

METHODS

Purification of Recombinant ATases

The cDNA cloning and overexpression of the human ATase has been

23 reported previously . Purification of the recombinant proteins was achieved either by affinity chromatograp ough a DNA-cellulose column as described by Wilkinson et al- « , or by DEAE-cellulose ion-exchange chromatography. For the latter, the ATase protein was partially purified by ammonium sulphate precipitation (30 - 60%) and dialysed against 10 mM Tris-HCl pH 7.5, 1 M DTT, 2 mM EDTA, 10% glycerol, before loading on a DEAE-cellulose column. The ATase was then eluted with a 0-0.1 M NaCl gradient. The purified human ATase protein retained activity for more than one year when stored at high concentration at -20°C in buffer I [50 M-Tris/HCl (pH 8.3)/3 mM-dithiothreitol/1 mM-EDTA] and could be thawed and refrozen several times without substantial loss of activity.

Incubation with Inactivators and ATase Assay

Compounds to be tested were dissolved in DMSO to a final concentration of 10 M and diluted just before use in buffer I containing 1 mg/ml bovine serum albumin (IBSA). Recombinant ATase was diluted in IBSA and titrated in order that the reaction be conducted under ATase, and not substrate, limiting conditions. In each assay, fixed amounts of ATase (60-75 fmol) were incubated with varying amounts of 0^-benzylguanine, or test compound in a total volume of 200 μl of IBSA containing 10 μg of calf thy us DNA at 37°C for 1 hour. The [ ³ H]-methylated-DNA substrate (100 l containing 6.7 μg of DNA and 100 fmol of () -methylguanine) was added and incubation continued at

37° for 1 hour, until the reaction was completed. Following acid

21 hydrolysis of the DNA as previously described the

[ ³ H]-methylated protein was recovered and quantitated by liquid scintillation counting. Samples were typically assayed in duplicate and experiments repeated several times. I _™ is the concentration of inactivator required to produce a 50% reduction in ATase activity.

Cell Culture and preparation of extracts

Mammalian cells were cultured under standard conditions. For example, Raji (a human lymphoblastoid cell line from a Burkitt's lymphoma) cells were grown in suspension culture in RPMI medium supplemented

with 10% horse serum. A375M cells are human melanoma cells from which the xenografts described below were established following subcutaneous injection into nude mice: WiDr cells are a human colon carcinoma cell line: Hamster+120 cells are a mitozolomide-selected subline of a Chinese hamster lung fibroblast V79 cell line called RJKO: Yoshida cells are a rat carcinosarcoma cell line and R _bus is a busulphan resistant subline thereof: XP cells are an SV40-transformed fibroblast cell line originally from the skin of a Xeroder a piomentosum patient, pHMGhAT2b cells are a clone of these cells that have been transfected with a mammalian cell expression vector containing the human ATase cDNA and pHMGla cells are a clone that have been transfected with the expression vector only (i.e. one not containing the human ATase cDNA). Cell pellets were resuspended in cold (4°C) buffer I containing 2 μg/ l leupeptin and sonicated for 10 seconds at 12 J peak to peak distance. After cooling in ice, the cells were sonicated for a further 10 seconds at 18 ^μ. Immediately after sonication, 0.01 volumes of 8.7 mg/ml phenylmethanesulphonylfluoride in ethanol was added and the sonicates centrifuged at 15 000 g for 10 minutes at 4°C to pellet cell debris. The supernatant was kept for determination of ATase activity (see below).

Stability of Inactivators at 37 C.

Inactivators (lOmM in DMSO) were diluted to O.lmM in prewar ed degassed buffer I (ImM EDTA, 50mM Tris pH 8.3) or PBS (pH 7-7.2). PBS (Phosphate buffered saline) is 0.8% NaCl, 0.02% KC1, 0.15% Na ₂ H ₂ P0 ₄ , 0.02% KH ₂ P0 ₄ , pH 7.2. Samples were immediately transferred to a CARY13 spectrophoto eter (cuvette block held at 37°C) and scanned at an appropriate wavelength (according to the spectral properties of the compound) at 3-10 minute intervals for up to 80 hours. The results were expressed as percentage absorbance change versus time and Tl/2 values (half life) extrapolated from this.

Inactivation of ATase activity in mammalian cells. Cells were diluted to 10 /ml in culture medium containing either the appropriate concentration of inactivator or an equivalent volume of vehicle (DMSO). Following incubation at 37°C for 2 hours the cells were harvested by centrifugation, washed twice with PBS and the resulting cell pellets (between 1-2 x 10 per pellet) stored at -20°C.

ATase activity was determined as described above, in duplicate sonicated cell extracts and expressed as the percentage activity remaining based on that present in the untreated controls (for example 350-450 fm/ g in Raji cells). I _™ (i.e concentration of inactivator required to reduce ATase activity by 50%) values were extrapolated from this data.

Sensitization of Raji and A375H cells to BCNU and temozolomide.

Sensitization of Raji cells to the cytotoxic effects of BCNU and temozolomide following a 2 hour pretreatment with inactivator was

22 analysed using an XTT assay . Briefly, cells were plated at 1000 cells/well in 96 well plates and incubated at 37°C for 30 minutes prior to the addition of medium containing either the appropriate concentration of inactivator or an equivalent volume of vehicle. Following a 2 hour incubation at 37 C, medium containing either increasing doses of BCNU, temozolomide or equivalent vehicle was added and the cells allowed to grow for 6 days. At this time XTT solution was added and the cells incubated for a further 4 hours at 37°C. The resulting red/orange for azan reaction product was quantified by measuring absorption at 450nm on a microtitre platereader.

Sensitization of A375M cells to the cytotoxic effects of BCNU was

24 analysed by MTT assay differing from the XTT assay described above as follows. A375M cells (1000 per well) were allowed to grow for 24 hours then treated with the inactivator and 2 hours later with

BCNU. After 6 days MTT solution (4mg/ml) was added and cells incubated for 3 hours at 37°C. Medium was aspirated and the resulting purple formazan crystals were solubilised in DMSO (120μl) and cell viability was quantified by measuring absorption at 540nm using a microtitre plate reader.

From this data the percentage growth of cells relative to that in control wells was determined for a range of BCNU or temozolomide doses in both the presence and absence of inactivator. Raji sensitization to BCNU (D ₉₀ - ^C /D ₉₀ - ¹ ) was determined by dividing the D _gQ

(i.e. dose at which there was 90% growth versus untreated controls i.e. 10% growth inhibition) calculated for data on use of BCNU alone (D _Qn . ^C ) by that for BCNU plus inactivator (Dg _Q . ). A value of one (1) thus indicates no sensitization by the inactivator. Raji

sensitization to temozolomide and A375M sensitization to BCNU were determined using the corresponding values (i.e. the doses at which there was 50% growth inhibition).

Sensitivity of mammalian cells to the inactivators or their hydrolysis products

In order to assess the effects on cell growth alone, Xeroderma piαmentosu cells and Chinese Hamster V79 cells were exposed to increasing concentrations (up to 600μM) of selected inactivators for 2h at 37°. In some cases, the inactivators were allowed to undergo hydrolysis at 37°C for 20 hours and then added to Raji cells in order to assess the extent to which the decomposition products of the agents might inhibit cell growth. After 6 days the extent of cell growth was determined as described above.

Sensitization of bone marrow cells to temozolomide (GH-CFC assay)

For the granulocyte/macrophage colony-forming cell (GM-CFC) assay primary human bone marrow samples were obtained from patients undergoing cardiothoracic surgery. Following removal of erythrocytes from the samples, cells were plated out at 1-2 x 10 /ml in 300m0sM Iscoves medium containing lOμM inactivator or equivalent volume of DMSO, 20% foetal calf serum, 10% 5637 conditioned medium as a source of growth factors and 0.3% agar noble, in 1ml petri dishes containing appropriate dose of temozolomide and incubated at 37 C in an atmosphere of 5% C0 ₂ and 95% air. After 9 days, colonies comprising of more than 50 cells were counted. The colonies represented precursor cells of the granulocyte/macrophage lineage (huGM-CFC).

Survival was expressed as a % of the number of colonies at zero dose temozolomide.

Xenograft studies

Animals

BALB-C derived athymic male mice (nu/nu athymic) weighing between 25-35g were obtained from the in-house breeding colony of the Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Wilmslow Road, Manchester M20 9BX, England (ASU mice). The animals were housed ■in a sterile environment until required for experiments. In one

experiment mice were obtained from Harlan-Olac, Harlan UK Limited, Shaw's Farm, Blackthorn, Bicester, Oxon. 0X6 OPT, England (OLAC mice). These mice weighed between 17 - 23g and were subsequently found to be more sensitive to the toxic effects of the combination (inactivator and BCNU) treatment. For this reason lower doses were administered.

Cells

A375M (human melanoma) cells were grown in DMEM containing 10% fetal bovine serum. The cells were prepared for in vivo inoculation by incubation with 0.01% trypsin.

Tumours

Cells (10 ) in lOOul of PBS were injected subcutaneously into the right-hand flank of 8-10 week old nu/nu athymic mice. These cells were allowed to develop into a tumour for 3-4 weeks for passaging into experimental animals. Tumour blocks measuring 2mm x 2mm x 2mm were implanted subcutaneously on the right hand flank. These animals were ready for use in inactivator experiments in 7-10 days.

Drug Treatment

Nu/Nu mice were treated with either 30 or 60 mg/kg CJ -benzylguanine, or B.4205 and the appropriate vehicle control (i.p), 60 - 90 minutes prior to 10 - 25 mg/kg BCNU (i.p.). 0 -benzylguanine and B.4205 were prepared as a 5 mg/kg solution in corn oil and BCNU (2mg/ml) in PBS + 3% ethanol.

Tumour Measurements

Xenograft tumour measurements were taken every 1-2 days by workers using digital calipers. Tumour volume was calculated using the formula (h x w x l) V/6. The experimental animals were also weighed every 1-2 days. Measurements continued until tumours in the control animals reached the maximum allowable volume (i.e. 1cm x 1cm x 1cm).

RESULTS

Tables 4, 5 and 6 shows the physical, biochemical and in vivo data for each of the inactivators. The tests listed are explained in the Methods section.

TABLE 4 INACTIVATOR T1/2(h) Raji sensitization (Dgo-C/Dgo- ¹ ) in PBS O.lμM 0.5μM 1.0μ

0_ ⁶ -benzylguanine > 16 *1.9+ 0.7 *2.0 + 0.4 *3.8 + 1

0 ⁶ -aliylguanine

B4203 O ⁶ -turfurylguanlne.1/2H ₂ 0 240 0.08 0.3 0.04 0.17 2.6 5.7

B.4205 0 ⁶ -thenylguanine.1/3EtOH 262 0.018 1.2 0.02 0.6 8.4 8.0

B.4206 0 ⁶ -3-thienylmethylgιιanlne.1 MeOH 279 0.03 >80 0.06 > 16 1.6 6.0

B.4209 0 ⁶ -3-furylmethylguanine 231 0.15 0.15 > 16 1.5 1.4 2.8

B.4210 0 ⁶ -2-picolylguanine.1/2H ₂ 0 251 35 > 1.0 > 1β

B.4211 0 ⁶ -3-picolylguanine.1/2H ₂ 0 251 0.43 > 16

B.4212 _ ⁶ -piperonylguanlne.3/4H ₂ 0 299 0.02 1.3 0.05 0.72 2.7 10.7

B.4213 0 ⁶ -(2-naphthylmethyl)guanine 291 0.15 0.03 > 16 1.2 2.1 2.9

*Mean of 8 individual experiments

TABLE 4(continued) INACTIVATOR M.Wt l ₅₀ (μM) T1/2(h) Raji l ₅₀ T1/2(h) Rail sensitization In Buffer I (μM) In PBS O.lμM 0.

B.4214 DL-0 ⁶ -(d-methyibenzyl)guanine 255 > 60

B.4217 DL-C) ⁶ -((t-methy.thenyl)guanine 261 > 500

B.4218 (D ⁶ -(2-methylbenzyl)guanlne 255 58

B.4219 DL-C) ⁶ -[1-(3-thlenyl)ethyl]guanlne 261 85 > 1.0 0.12 1.0 1.0 1.0

B.4220 C) ⁶ -(5-methylthenyl)guanine 261 > 1.0 > 16 1.0 1.0 1.0

B.4221 0 ⁶ -(5-methyiturfuryl)guanlne.1/2H ₂ 0 254 10 > 1.0 > 16 1.0 1.0 1.0

B.4222 (D ⁶ -(3-methylthenyl)guanine 260 0.65 0.47 > 16 1.4 1.9 2.1

B.4226

0 ⁶ -(2-benzoIb]thienylmethyl) guanine.1 MeOH 329 0.03 9.7 0.06 6.7 1.6 1.6 10.0

B.4229

(D ⁶ -(5-ιτιethoxycarbonylfurfuryl) guanine.1.5H2θ 316 0.09 0.25 > 16 1.2 2.1 2.5

TABLE 4(continued) INACTIVATOR M.Wt <5o(μM) T1/2(h) Raji l ₅₀ Tl/2(h) Raji sensitization (Dgo^/Dgg. ¹ ) in Buffer I (μM) In PBS 0.1μM 0.5μM 1.0μM

B.4231 0 ⁶ -(2-methoxybenzyl)guanine.1/2H2θ 280 60

B.4234 () ⁶ -(5-carboxyfurfuryl)guanine 306 60 > 1.0 > 1β 1.0 1.0 1.0

B.4265

(3 ⁶ -1-(πaphthylmethyl)guanlne

.1/2MeOH 307 95 60

B.4266

0 ⁶ -(2-benzofuranylmethyl)guanine

.IMeOH 313 0.035 > 16 c

B.4268 0_ ⁶ -piperonylguanoslne.2H ₂ 0 453 0.75

B.4269 () ⁶ -(5-bromothenyl)guanlne 326 0.0045 0.006

B.4271

( ⁾⁶ -(-5-azapiperonyl)guanine

.1/4EtOH 298 0.23 > 16

B.4273 O ^β -(5-cyanofurfuryl)guanine.H ₂ 0 256 0.006

B.4274

0ι6-{5-oxazolylmethyl)guanlne.1/4

H ₂ 0 274 0.34

TABLE 4(continued) INACTIVATOR M.Wt l ₅₀ (μM) T1/2(h) Raji l ₅₀ T1/2(h) Raji sensitization (Dgo.C Dg _Q . ¹ ) in Buffer I (μM) in PBS O.lμM 0.5μM 1.0μM

B.4275

0 ⁶ -(5-thiazolylmethyl)guanine.1/2

H ₂ 0 257 0.033

B.4276

(3 ⁶ -(2-benzo[b]thlenylmethyl) guanosine.H ₂ 0 464 0.35

B.4277 0 ⁶ -4-picolylguanine 242 0.13

B.4278

0 ⁶ -(1-methyl-4-nitro- pyrrol-2-ylmethyl)guanlne 298 0.55

B.4279 0 ⁶ -thenylguanosine.1/2H2θ 388 0.9

Table 5

Inactivation of ATase in Mammalian Cells l5o( M)

CELL LINE Hamster Rat Human Human Human

INACTIVATOR + 120 Yoshida XP Raji WiDr

θ6-benzylguanine 0.20 0.14 0.07 0.10 >0.09

B.4203 0.12 0.06 0.06 0.04 0.08

B.4205 0.03 0.02 0.03 0.02 0.02

Table 6

A375M sensitization (DSO.VDSQ. ¹ )

INACTIVATOR 0.5uM l.OuH 5.0uH

()6-benzylguanine 3.1 2.3 4.2 ()6-allylguanine 1.3 l.g

B.4203 2.0 2.5

B.4205 2.8 2.3 3.5

B.4206 3.3 4.6 B.4209 2.5

B.4210 1.5

B.4212 2.3 2.5

B.4213 2.0

B.4220 1.3

B.4221 1.0

B.4222 1.4

B.4226 3.8 4.5

B.4229 1.8

B.4234 0.8

B.4266 6.3 6.3

Figure 1 shows the result of the jji vitro ATase inactivation assay using 4 of the compounds. B.4206 was somewhat more effective than BeG but B.4212 and B.4205 were considerably better under the assay conditions used. B.4203 was not as effective as BeG.

Figure 2 shows that at O.luM inactivator, B.4205 was more effective than BeG in sensitizing Raji cells to the growth inhibitory effects of BCNU: at l.OuM inactivator, B.4205 and BeG were equally effective in this respect.

Figure 3 shows that at O.luM inactivator, B.4205 was more effective than BeG in sensitising Raji cells to the growth inhibitory effects of temozolomide but that as the doses of the inactivators were increased, sensitization by BeG became more effective whilst that by

B.4205 remained the same. This lack of dose response with B.4205 but clear dose response with BeG is shown more clearly in Figure 4.

Figure 5 shows that whilst some growth inhibition of B79 cells was produced by B.4203 at doses in excess of lOOuM (i.e. at least lOOx higher than the I _™ dose for this compound ), no such effects were seen with B.4205 up to the maximum concentration used.

Figure 6 shows that XP cells were as susceptible to the growth inhibitory effects of B.4203 but that these cells were more sensitive than V79 cells to the effects of B.4205. However, the doses required for growth inhibition were at least 100 times that required for ATase inactivation. It can be concluded that the inherent growth inhibitory effects of these inactivators would not contribute detectably to the sensitization of cells to the growth inhibitory effects of the alkylating agents.

Figure 7 shows that no substantial growth inhibitory effects were produced in Raji cells when the given compounds were allowed to undergo hydrolysis (see Methods) before being added to the cells without further addition of alkylating agents. Under these experimental conditions therefore, the decomposition products of the agents would not be expected to contribute to the growth inhibitions seen using combinations of these agents and alkylating agents.

Figure 8 - 13 shows the results of the xenograft study in greater detail. The depletion and recovery of ATase activity following exposure to 0 -benzylguanine or B.4205 was measured in A375 tumour xenograft (Fig.8) extracts prepared from tissues of animals sacrificed 2 or 24 hours after administration of inactivator. Figures 9-13 indicate the sensitization of A375 tumour xenografts in nude mice to either 0. -benzylguanine or B.4205 in combination with BCNU as described in the Methods section. In each Figure the top graph shows the percentage increase in tumour volume over the time course of the experiment. The lower graph shows the number of animals in each treatment group and the number surviving following treatment. The graphs show that B.4205 is comparable to 0 -benzylguanine (BeG) in reducing tumour volume and is substantially less toxic in

combination with BCNU than BeG in combination with BCNU. In human patients, cutaneous malignant melanoma is treated with BCNU, particularly in the USA (Balch, C ., Houghton, A. & Peters, L. (1989) "Cutaneous Melanoma" in Cancer: Principles and Practise of Oncology, De Vita, V.T., Helman, S. & Rosenberg, S.A. (eds), ppl499-1542,

Lipincott: Philadelphia) so that the human melanoma xenograft grown in nude mice is an animal model system that is clinically highly relevant.

Figure 14 shows the results of the tests on sensitization of human bone marrow cells to temozolomide as described in the Methods section above. The bone marrow cells were obtained from three patients identified as C, D and E respectively. The survival curves shown relate to the cytotoxic effect of combined inactivator/temozolomide treatment. It is desirable that this effect should be reduced. The results for patients C & E show that B.4205 had a smaller sensitization effect than 0. -benzylguanine (0 BeG); for patient D there was no differential but this result is regarded as a reasonable variation in scientific testing with human material.

Table 7 shows the toxic effect of inactivator alone on bone marrow cells obtained from 5 patients A - E, including the same patients C - E as in Figure 17. The results for B.4205 are comparable to those for 0 -benzylguanine. Note that the cells of patient B were almost twice as sensitive to both BeG and B.4205 in comparison to the other samples.

Table 7

INACTIVATOR (10uM) TOXICITY IN BONE MARROW CELLS

Patient Date of Experiment No. of colonies as % of control (ie no inactivator) Q -benzylguanine B.4205

95 91 46 46 88 97 88 118 89 89

Summary of Findings

1) All of the compounds have been tested for their ability to inactivate recombinant human ATase under standard conditions in an in vitro assay. Under these conditions two of the compounds (B.4214 and B.4217) did not inactivate ATase up to the highest concentration used but these were compounds in which R' is methyl. The remainder of the compounds inactivated ATase with Igg values ranging from 0.0045 to 95|imolar. Eight compounds (B.4205, B.4206, B.4212, B.4226, B.4266, B.4269, B.4273, B.4275) had I _5Q values lower than that of BeG (Table 4).

2) The inactivators underwent hydrolysis in aqueous solution at different rates (half lives 0.17 to>80 hours) but this was not related to their efficiency as ATase inactivators. (Table 4)

3) Compounds that were efficient in the inactivation of ATase in vitro (I _ςn <1.0μM) also inactivated ATase in human cells with I _™ values that were generally only slightly (mean approx. 1.5 times) higher than those found using recombinant protein in the in vitro assay. (Table 4)

4) B.4205 inactivated ATase in human, rat and Chinese hamster cells with similar effectiveness (I _5Q values 0.02-0.03). For B.4203 the range was 0.04-0.12. In the cell lines used B.4205 and B.4203 were up to 7 times more effective than BeG. (Table 5)

5) B.4203 and B.4205 were toxic to the XP cells studied and B.4203 was toxic to the V79 cells studied but only at concentrations that were at least 100 times higher than those at which sensitization to BCNU was observed. (Figs 5 & 6)

6) Compounds that were efficient in the inactivation of ATase in vitro (I _rn <1.0/iM) and in Raji cells (I _5Q <1.0/iM) also sensitized Raji and A375M cells to the growth inhibitory effects of BCNU where this was tested. (Table 4)

7) At inactivator concentrations of O.l M, B.4205 was more effective than BeG in sensitising Raji cells to the growth inhibitory effects of temozolomide. (Fig 3)

8) The in vitro assay can be used to predict which compounds are most likely to be effective sensitisers of mammalian cells to the growth inhibitory effects of BCNU and related cytotoxic agents.

9) B.4205 was similar or slightly more effective than BeG in sensitising human melanoma xenografts grown in nude mice to the growth inhibitory effects of BCNU. (Figs. 9-13)

10) B.4205 was as effective as BeG in inactivating ATase in human melanoma xenografts grown in nude mice. (Fig. 8)

11) The in vitro assay and/or the xenograft ATase depletion assay may be used to predict which compounds are most likely to be effective sensitisers of melanoma xenografts to the growth inhibitory effects of BCNU and related cytotoxic agents.

12) In contrast to BeG, which caused death in up to 70% of the treated animals, B.4205 had very little effect on the sensitivity of nude mice bearing human melanoma xenografts to the acute toxic effects of BCNU under the conditions used. (Figs. 9-13)

13) BeG sensitised the GM-CFCs in the three human bone marrow samples tested to the toxic effects of temozolomide but in two of these samples, little or no sensitization was produced by B.4205. This assay may therefore be used to predict the possible myelosuppressive effects of ATase inactivators when used in the clinic in combination with BCNU and related agents. (Fig. 14)

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In the specification the abbreviations "lh" or "2h" etc. mean "1 hour", "2 hours", etc.