Title:
PLASMID PLATFORM FOR STABLE EXPRESSION AND DELIVERY OF BIOMOLECULES
Document Type and Number:
WIPO Patent Application WO/2023/038479
Kind Code:
A1
Abstract:
A plasmid platform according to the present invention comprises a nucleic acid sequence encoding a modified protein from which an intracellular domain, an extracellular domain, or a combination thereof of lysosome-associated membrane glycoprotein 2B (LAMP-2B) has been removed, and is based on the discovery that removing a specific domain of LAMP-2B significantly stabilizes the expression of intended biomolecules and significantly increases the efficiency with which intended biomolecules are delivered. The plasmid platform is expected to provide high technical utility in the expression and delivery of proteins of interest, particularly expression and delivery using exosomes, and may be expanded to various associated technical fields or uses.
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Inventors:
JANG KANG WON (KR)
CHOI JONG HYUN (KR)
CHOI JONG HYUN (KR)
Application Number:
PCT/KR2022/013574
Publication Date:
March 16, 2023
Filing Date:
September 08, 2022
Export Citation:
Assignee:
KKOOMLAB LNC (KR)
International Classes:
C12N15/63; A61K48/00; A61P35/00; C07K14/705; G01N33/574
Foreign References:
| US20180104187A1 | 2018-04-19 | |||
| US20200390700A1 | 2020-12-17 | |||
| KR20210030965A | 2021-03-18 |
Other References:
LYDIA ALVAREZ-ERVITI, YIQI SEOW, HAIFANG YIN, CORINNE BETTS, SAMIRA LAKHAL, MATTHEW J A WOOD: "Delivery of siRNA to the mouse brain by systemic injection of targeted exosomes", NATURE BIOTECHNOLOGY, GALE GROUP INC., vol. 29, no. 4, 1 April 2011 (2011-04-01), pages 341 - 345, XP055089953, ISSN: 10870156, DOI: 10.1038/nbt.1807
LI ZHELONG, ZHOU XUEYING, GAO XIAOTONG, BAI DANNA, DONG YAN, SUN WENQI, ZHAO LIANBI, WEI MENGYING, YANG XUEKANG, YANG GUODONG, YUA: "Fusion protein engineered exosomes for targeted degradation of specific RNAs in lysosomes: a proof‐of‐concept study", JOURNAL OF EXTRACELLULAR VESICLES, TAYLOR & FRANCIS, UK, vol. 9, no. 1, 1 September 2020 (2020-09-01), UK , pages 1816710, XP093045220, ISSN: 2001-3078, DOI: 10.1080/20013078.2020.1816710
MICHELLE E. HUNG ET AL., THE JOURNAL OF BIOLOGICAL CHEMISTRY, 27 March 2015 (2015-03-27)
LI ZHELONG, ZHOU XUEYING, GAO XIAOTONG, BAI DANNA, DONG YAN, SUN WENQI, ZHAO LIANBI, WEI MENGYING, YANG XUEKANG, YANG GUODONG, YUA: "Fusion protein engineered exosomes for targeted degradation of specific RNAs in lysosomes: a proof‐of‐concept study", JOURNAL OF EXTRACELLULAR VESICLES, TAYLOR & FRANCIS, UK, vol. 9, no. 1, 1 September 2020 (2020-09-01), UK , pages 1816710, XP093045220, ISSN: 2001-3078, DOI: 10.1080/20013078.2020.1816710
MICHELLE E. HUNG ET AL., THE JOURNAL OF BIOLOGICAL CHEMISTRY, 27 March 2015 (2015-03-27)
Attorney, Agent or Firm:
INNOBANK PATENT LAW FIRM (KR)
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