POLYCISTRONIC EXPRESSION VECTOR IN PLANT

WIPO Patent Application WO/2019/083308

A1

The present invention relates to a cloning vector carrying a novel non-mammalian virus derived 2A sequence, a polynucleotide for biosynthesis of carotenoid, and a transgenic plant. More particularly, the present invention relates to a cloning vector carrying a non-mammalian virus-derived 2A sequence, a polynucleotide encoding phytoene synthase, non-mammalian virus-derived 2A sequence, carotene desaturase, β-carotene hydroxylase, and β-carotene ketolase, a recombinant expression vector carrying the polynucleotide, a transformed cell having the polynucleotide introduced thereinto, and a transgenic plant having the polynucleotide introduced thereinto. A 2A sequence according to the present invention exhibits higher ribosomal skipping efficiency than conventional FMDV-derived 2A sequences, thereby enabling the development of transgenic plants that are of high genetic expression stability. In addition, a ribosomal skipping 2A sequence according to the present invention is derived from a non-mammalian virus and thus expected to increase popular acceptance upon the development of transgenic plants.

HA SUN HWA (KR)
JEONG YE SOL (KR)
KU HYUNG KEUN (KR)
YOU MIN KYOUNG (KR)

PCT/KR2018/012762

May 02, 2019

October 25, 2018

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UNIV INDUSTRY COOPERATION GROUP KYUNG HEE UNIV (KR)

C12N9/10; C07K14/005; C12N9/02; C12N15/62; C12N15/82

KR101229887B1	2013-02-08
KR20120045631A	2012-05-09
KR20130022520A	2013-03-07
KR20180046379A	2018-05-08

LUKE, G. A. ET AL.: "The Protein Coexpression Problem in Biotechnology and Biomedicine: Virus 2A and 2A-like Sequences Provide a Solution", FUTURE VIROLOGY, vol. 8, no. 10, 2013, pages 983 - 996

HANA IP LAW FIRM (KR)

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