Title:
POLYPEPTIDES HAVING DEMETHYLATING ACTIVITY
Document Type and Number:
WIPO Patent Application WO/2016/207351
Kind Code:
A3
Abstract:
The present invention relates to fungal enzymes, more in particular of polyphenoloxidases (PPOs), and is based on a newly discovered enzymatic activity of a class of PPOs, i.e. de-methylation of R-substituted mono-or di-methoxyphenolsuch as present in lignin, lignin derived compounds and/or in lignocellulosic biomass. This newly discovered enzymatic activity renders these enzymes highly suitable for a plethora of applications in industry. Provided herein are methods of demethylation, processes to increase the reactivity of lignin or lignin-comprising biomass, processes of conversion lignin or lignin comprising biomass to value added products, processes for degrading and/or modifying (hemi-)cellulose in a hemicellulose-comprising substrate, and expression vectors, host cells and liquids, pastes or solid formulations and compositions for use in the demethylation method of the invention.
Inventors:
KOETSIER MARTIJN JOHANNES (NL)
VISSER JACOB (NL)
IANCU SVETLANA LAURA (NL)
KABEL MIRJAM ANNA (NL)
FROMMHAGEN MATTHIAS (NL)
GRUPPEN HARM (NL)
LANGE HEIKO (IT)
CRESTINI CLAUDIA (IT)
BENJELLOUN-MLAYAH BOUCHRA (FR)
VISSER JACOB (NL)
IANCU SVETLANA LAURA (NL)
KABEL MIRJAM ANNA (NL)
FROMMHAGEN MATTHIAS (NL)
GRUPPEN HARM (NL)
LANGE HEIKO (IT)
CRESTINI CLAUDIA (IT)
BENJELLOUN-MLAYAH BOUCHRA (FR)
Application Number:
PCT/EP2016/064666
Publication Date:
March 02, 2017
Filing Date:
June 24, 2016
Export Citation:
Assignee:
GENENCOR INT BV (NL)
International Classes:
C12P7/22; C02F3/00; C11D3/386; C12N9/02; C12P7/66; D06M16/00; D21H17/00
Domestic Patent References:
| WO2014081700A1 | 2014-05-30 | |||
| WO2012068236A2 | 2012-05-24 | |||
| WO2015035029A1 | 2015-03-12 | |||
| WO2014186567A1 | 2014-11-20 | |||
| WO2008134259A1 | 2008-11-06 |
Other References:
RAHOUTI M ET AL: "METABOLISM OF FERULIC ACID BY PAECILOMYCES-VARIOTI AND PESTALOTIA-PALMARUM", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 55, no. 9, 1989, pages 2391 - 2398, XP002755557, ISSN: 0099-2240
Attorney, Agent or Firm:
ANDREWS, Robert, K. (GB)
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