Title:
PREPARATION METHOD FOR INTERFERING PEPTIDE TARGETING SARS-COV-2 N PROTEIN AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2022/227702
Kind Code:
A1
Abstract:
Disclosed is a preparation method for an interfering peptide targeting a SARS-CoV-2 N protein, the method comprising the following steps: designing an interfering peptide fragment to target amino acids located in a dimerization domain of the SARS-CoV-2 N protein; fusing the interfering peptide fragment with HIV-TAT; modifying the interfering peptide fragment fused with HIV-TAT into a reverse isomer to obtain an amino acid sequence of a final interfering peptide NIP-V; and synthesizing the interfering peptide NIP-V by using D-amino acids as raw materials. The foregoing interfering peptide drug NIP-V can interact with the dimerization domain of the SARS-CoV-2 N protein, inhibit the oligomerization of the N protein, and then relieve the inhibition of the innate immunity by the N protein, thereby achieving the purpose of inhibiting the replication of SARS-CoV-2 virus in cells and animals.
Inventors:
ZHOU FANGFANG (CN)
DAI TONG (CN)
WANG SHUAI (CN)
ZHANG LONG (CN)
DAI TONG (CN)
WANG SHUAI (CN)
ZHANG LONG (CN)
Application Number:
PCT/CN2022/071356
Publication Date:
November 03, 2022
Filing Date:
January 11, 2022
Export Citation:
Assignee:
UNIV SOOCHOW (CN)
International Classes:
C07K19/00; A61K38/16; A61P31/14; C07K1/00; C07K14/00
Domestic Patent References:
| WO2005002500A2 | 2005-01-13 |
Foreign References:
| CN113072623A | 2021-07-06 |
Other References:
WANG SHUAI; DAI TONG; QIN ZIRAN; PAN TING; CHU FENG; LOU LINGFENG; ZHANG LONG; YANG BING; HUANG HUIZHE; LU HUASONG; ZHOU FANGFANG: "Targeting liquid-liquid phase separation of SARS-CoV-2 nucleocapsid protein promotes innate antiviral immunity by elevating MAVS activity", NATURE CELL BIOLOGY, vol. 23, no. 7, 1 July 2021 (2021-07-01), London, pages 718 - 732, XP037508071, ISSN: 1465-7392, DOI: 10.1038/s41556-021-00710-0
Attorney, Agent or Firm:
SUZHOU FLY PATENT AGENCY (GENERAL PARTERNERSHIP) (CN)
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