Title:
PRIMARY GASTROINTESTINAL STROMAL TUMOR CELL CULTURE MEDIUM, CULTURE METHOD, AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2022/151520
Kind Code:
A1
Abstract:
The present invention provides a cell culture medium for culturing primary gastrointestinal stromal tumor cells, comprising gastrin, N2, insulin, a receptor tyrosine kinase ligand, and a Rock kinase inhibitor. The present invention further provides a method for culturing gastrointestinal stromal tumor cells by using the cell culture medium, and an application of an expanded cell population, which is obtained by using the method, in efficacy evaluation or screening.
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Inventors:
LIU QINGSONG (CN)
LIU FEIYANG (CN)
LI XIAOYU (CN)
MEI HUSHENG (CN)
WANG WENCHAO (CN)
REN TAO (CN)
WANG LI (CN)
LIU FEIYANG (CN)
LI XIAOYU (CN)
MEI HUSHENG (CN)
WANG WENCHAO (CN)
REN TAO (CN)
WANG LI (CN)
Application Number:
PCT/CN2021/073246
Publication Date:
July 21, 2022
Filing Date:
January 22, 2021
Export Citation:
Assignee:
PRECEDO PHARMACEUTICALS CO LTD (CN)
International Classes:
C12N5/00; C12N5/09
Foreign References:
| CN106834212A | 2017-06-13 | |||
| CN106967672A | 2017-07-21 | |||
| US20170191030A1 | 2017-07-06 | |||
| CN103333858A | 2013-10-02 |
Other References:
ZHOU FANG-YING;FENG HAI-LIANG;BIAN XIAO-CUI;KANG WEI-MING;YU JIAN-CHUN;ZONG JING;ZHANG DAN;LIU YU-QIN: "Establishment of GIST Cell Line and Its Sensitivity to C-KIT Downstream Inhibitors", CHINESE JOURNAL OF CANCER PREVENTION AND TREATMENT, vol. 26, no. 11, 14 June 2019 (2019-06-14), pages 764 - 770+777, XP055951949, DOI: 10.16073/j.cnki.cjcpt.2019.11.003
XU ET AL., JOURNAL OF HEMATOLOGY & ONCOLOGY, vol. 11, 2018, pages 116
LIU ET AL., AM J PATHOL, vol. 183, no. 6, 2013, pages 1862 - 1870
LIU ET AL., THER ADV MED ONCOL, vol. 11, 2019, pages 1 - 15
FUKUDA ET AL., ONCOLOGY REPORTS, vol. 30, 2013, pages 71 - 78
ZHU ET AL., JOURNAL OF CENTRAL SOUTH UNIVERSITY, vol. 35, no. 11, 2010, pages 1138 - 1144
LIU ET AL., NAT PROTOC., vol. 12, no. 2, 2017, pages 439 - 451
GREENWOOD ET AL., ENVIRON MOL MUTAGEN, vol. 43, no. 1, 2004, pages 36 - 44
HANS CLEVERS ET AL., CELL, 11, vol. 172, no. 1-2, 2018, pages 373 - 386
XU ET AL., JOURNAL OF HEMATOLOGY & ONCOLOGY, vol. 11, 2018, pages 116
LIU ET AL., AM J PATHOL, vol. 183, no. 6, 2013, pages 1862 - 1870
LIU ET AL., THER ADV MED ONCOL, vol. 11, 2019, pages 1 - 15
FUKUDA ET AL., ONCOLOGY REPORTS, vol. 30, 2013, pages 71 - 78
ZHU ET AL., JOURNAL OF CENTRAL SOUTH UNIVERSITY, vol. 35, no. 11, 2010, pages 1138 - 1144
LIU ET AL., NAT PROTOC., vol. 12, no. 2, 2017, pages 439 - 451
GREENWOOD ET AL., ENVIRON MOL MUTAGEN, vol. 43, no. 1, 2004, pages 36 - 44
HANS CLEVERS ET AL., CELL, 11, vol. 172, no. 1-2, 2018, pages 373 - 386
Attorney, Agent or Firm:
SHANGCHENG & PARTNERS (CN)
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