PRODRUGS OF CANNABIDIOL, COMPOSITIONS COMPRISING PRODRUGS OF CANNABIDIOL AND METHODS OF USING THE SAME

CROSS-REFERENCE TO RELATED APPLICATIONS

[0001] This application claims the benefit of U.S. Provisional Application Ser. No. 601952,146 filed on July 30, 2007 and is hereby incorporated by reference.

FIELD

[0002] Described herein are pharmaceutically active agents (e.g., prodrugs of cannabidiol) suitable for local and systemic delivery to a mammal, including systemic transdermal delivery and topical delivery; compositions for delivering pharmaceutically active agents both systemically and locally; and the use of such compositions in treating and preventing diseases and disorders, as well as improving cosmetic appearance.

BACKGROUND

[0003] The clinical usefulness of the cannabinoids, including cannabidiol ("CBD"), to provide analgesia and neuroprotection, reduce inflammation, help alleviate nausea and emesis, as well as treat epilepsy, anxiety disorders, and glaucoma, has been well-recognized. In addition, it is also well-known that cannabidiol lacks the psychoactive effects seen in many of the other cannabinoids, including δ ⁹ -tetrahydrocannabinol, which is currently available in an oral dosage, sold under the trade name Marinol ^® .

[0004] Pain is the most frequently reported symptom and it is a common clinical problem which confronts the clinician. Millions of people in the USA suffer from severe pain that, according to numerous recent reports, is chronically under-treated or inappropriately managed. Similarly, millions of people also suffer from severe nausea and/or frequent emesis. Moreover, all too frequently, many patients suffering from chronic, under-treated or unretractable pain also suffer from lack of appetite, nausea and/or frequent emesis, such that a patient is unable to receive effective therapeutic doses of oral pain medications, thereby exacerbating their pain. Cannabinoids, including cannabidiol, are effective in alleviating pain. Moreover, cannabinoids, including cannabidiol, can reduce a patient's nausea and vomiting, independent of any pain relief achieved. Thus cannabinoids are particularly useful in patients experiencing nausea and vomiting secondarily to un- or under-treated pain.

[0005] A notable percentage of the U.S. population satisfy the diagnostic criteria for alcohol use disorders ("AUDs"). The consumption of excessive amounts of alcohol results in a complex array of pharmacological effects that directly impact the ability to treat the condition. These effects directly impact the brain and include progressive neurodegeneration, impaired executive function and dependence leading to withdrawal-induced negative effects. It is known that the cannabinoids, including cannabidiol, have neuroprotective, anxiolytic and anti-convulsant effects, which may be effective in preventing additional brain damage in persons with AUDs, while simultaneously decreasing the frequency of relapses.

[0006] Dystonia is a neurological movement disorder, with many known causes, and characterized by involuntary, continual muscular contractions causing twisting and repetitive movements or abnormal postures. Cannabinoids have been shown to reduce the symptoms of muscular contractions characterizing this disorder.

[0007] The etiological pathology of many diseases relates to the inflammatory processes caused by an individual's immune system. The inflammation may result from (1) an otherwise appropriate immunoresponse to an outside trauma, such as brain swelling secondary to a closed head injury; (2) an overactive immunoresponse such as with an allergic reaction or dermatitis; or (3) an inappropriate auto- immunoresponse such as what causes certain forms of multiple sclerosis, inflammatory bowel disorders and arthritis. Regardless of the underlying cause of the inflammation, it is therapeutically desirable under these circumstances to regulate the immune system and lessen the inflammatory response. Cannabinoids have been shown to regulate various steps in the immune response and could show some therapeutic benefit in treatment of certain inflammatory diseases such as psoriatic arthritis.

[0008] Rheumatoid arthritis affects approximately 0.5-1% of the United States population, and autoimmune diseases in general affect more than 20 million Americans. The pain associated with rheumatoid arthritis can often be disabling. Cannabinoids have been found to be useful as adjunct treatment for rheumatoid arthritis and joint pain secondary to other autoimmune diseases, such as inflammatory bowel disease, multiple sclerosis and systemic lupus erythematosus.

[0009] In addition to the above-discussed therapeutics benefits, cannabinoids, cannabidiol, and cannabidiol prodrugs present a variety of pharmacological benefits, including, but not

limited to, anti- inflammatory, anti-convulsant, anti-psychotic, anti-oxidant, neuroprotective anticancer and immunomodulatory effects.

[0010] Given these systemic therapeutic benefits, it would be advantageous to develop a composition in which cannabidiol is delivered to achieve a therapeutically effective concentration in a patient. Unfortunately, as with the other cannabinoids, cannabidiol undergoes substantial first-pass metabolism when absorbed from the human gut after oral administration. Further, the oral bioavailability of any product is further diminished when a patient suffers from nausea or emesis, as either they avoid taking their oral medication or the oral dosage form does not remain in the GI tract for a sufficient amount of time to achieve a therapeutic dose.

[0011] Therefore, in view of the foregoing, it would be desirable to deliver therapeutically effective amounts of cannabidiol to a mammal in need thereof for the treatment of one or more medical conditions, such as pain, nausea or appetite stimulation, by a route of administration that does not depend upon absorption from the gastrointestinal tract of the mammal and not subject to first-pass metabolism upon absorption from the gastrointestinal tract. One non-oral route of administration for the systemic delivery of cannabidiol is transdermal.

[0012] Unfortunately, due to its highly hydrophobic nature, cannabidiol is poorly absorbed through membranes such as the skin of a mammal, such as a human. Therefore, the success of transdermally administering therapeutically effective quantities of cannabidiol to a mammal in need of such treatment within a reasonable time frame and over a suitable surface area has been substantially limited.

[0013] However, the epidermis and dermis of many mammals, such as humans and guinea pigs, contains enzymes which are capable of metabolizing active pharmaceutical agents which pass through the stratum corneum. The metabolic process occurring in the skin of mammals, such as humans, can be utilized to deliver pharmaceutically effective quantities of cannanbidiol to the systemic circulation of a mammal in need thereof. Described herein are prodrugs of cannabidiol that can be transdermally administered to a mammal, such as a human, so that the metabolic product resulting from metabolism in the skin is cannabidiol which is systemically available for the treatment of a medical condition such as pain, nausea or appetite stimulation. Also described herein are compositions comprising cannabidiol prodrugs suitable for transdermal delivery to a mammal in need thereof and methods of using cannabidiol prodrugs.

[0014] Therefore, a significant advancement in the art would occur with the development of a cannabidiol prodαig capable of transdermal delivery; compositions suitable for transdermal delivery comprising prodrugs of cannabidiol; and methods of using prodrugs of cannabidiol whereby the resulting metabolic product was cannabidiol which is locally or systemically available to a mammal in a therapeutically effective amount.

[0015] In addition, pharmaceutical compositions can be systemically administered by other means, including: oral, buccal, sublingual, injection, rectal, vaginal and intranasal. The metabolic process occurring in mammals, such as humans, can also be utilized to deliver pharmaceutically effective quantities of cannabidiol to the systemic circulation of a mammal in need thereof. Described herein are prodrugs of cannabidiol that can be administered to a mammal, such as a human, so that the metabolic product resulting from metabolism in the skin is cannabidiol which is available for the treatment of a medical condition such as pain, nausea or appetite stimulation. Also described herein are compositions comprising cannabidiol prodrugs suitable for delivery to a mammal in need thereof and methods of using cannabidiol prodrugs.

[0016] Therefore, a significant advancement in the art would occur if one could develop a prodrug of cannabidiol capable of oral, buccal, sublingual, injectable, topical, follicular, nasal, ocular, rectal or vaginal delivery; compositions suitable for oral, buccal, sublingual, injectable, topical, follicular, nasal, ocular, rectal, vaginal delivery comprising prodrugs of cannabidiol; and methods of using prodrugs of cannabidiol whereby the resulting metabolic product was cannabidiol which is systemically available to a mammal in a therapeutically effective amount.

[0017] In addition to the benefits of systemically administered cannabidiol discussed above, cannabinoids, including cannabidiol, have been found to have localized benefits from topical administration. For example, topically administered cannabinoids have been found to be useful to alleviate pain and other conditions originating near the surface of the skin, including but not limited to pain associated with post-herpetic neuralgia, shingles, burns, actinic keratosis, oral cavity sores and ulcers, post-episiotomy pain, psoriasis, pruritis, contact dermatitis, eczema, bullous dermatitis herpetiformis, exfoliative dermatitis, mycosis fungoides, pemphigus, severe erythema multiforme (e.g., Stevens-Johnson syndrome), seborrheic dermatitis and psoriatic arthritis. In addition, topically administered cannabinoids have been found to be useful to alleviate pain and other conditions associated with deeper tissues, such as peripheral neuropathic

pain, including but not limited to the peripheral neuropathic pain associated with diabetic neuropathy, ankylosing spondylitis, Reiter's syndrome, gout, chondrocalcinosis, joint pain secondary to dysmenorrhea, fibromyalgia, musculoskeletal pain, neuropathic-postoperative complications, polymyositis, acute nonspecific tenosynovitis, bursitis, epicondylitis, posttraumatic osteoarthritis, synovitis, and juvenile rheumatoid arthritis. When cannabinoids are administered topically to treat pain and other conditions associated with deeper tissues, including peripheral neuropathic pain, it maybe useful to co-administer cannabinoids systemically. Also, it has been found that the topical administration of cannabinoids, including cannabidiol, can inhibit the growth of hair.

[0018] In order to achieve these local benefits, it is advantageous for cannabidiol or a prodrug thereof to penetrate the stratum corneum but not be absorbed systemically. In such a case, the cannabidiol would concentrate in the skin and/or pilosebaceous unit, thus maximizing its local effect. Not only does the localized effect increase the potential therapeutic benefit, it lessens the frequency and severity of side-effects associated with cannabinoid administration because the amount of active compound circulating in the patient is minimized. The cannabidiol can be incorporated into a prodrug with an active moiety that would improve the appearance and/or hydration of the skin.

[0019] Therefore, a significant advancement in the art would occur with the development of a cannabidiol prodrug capable of topical delivery, such that it penetrates the outer layer of the skin but is not absorbed into circulation; compositions suitable for topical delivery comprising prodrugs of cannabidiol and methods of using prodrugs of cannabidiol whereby the resulting metabolic product was cannabidiol which is available at the site of administration in a mammal in a therapeutically effective amount but is not absorbed systemically.

SUMMARY

[0020] Described herein are prodrugs of cannabidiol, methods of making prodrugs of cannabidiol, compositions comprising prodrugs of cannabidiol and methods of using prodrugs of cannabidiol.

[0021] Other embodiments, objects, features and advantages will be set forth in the detailed description of the embodiments that follows, and in part will be apparent from the description, or may be learned by practice, of the claimed invention. These objects and advantages will be

realized and attained by the processes and compositions particularly pointed out in the written description and claims hereof. The foregoing Summary has been made with the understanding that it is to be considered as a brief and general synopsis of some of the embodiments disclosed herein, is provided solely for the benefit and convenience of the reader, and is not intended to limit in any manner the scope, or range of equivalents, to which the appended claims are lawfully entitled.

BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 is a line graph illustrating the representative permeation profile of cannabidiol (n=3) and ALL00105 (n=4) in a gel formulation, wherein "n" is the number of skin samples tested.

[0023] Figure 2 is a line graph illustrating the representative permeation profile of cannabidiol (n=2) and ALL00105 (n=2) in a gel formulation, wherein "n" is the number of skin samples tested.

[0024] Figure 3 is a bar graph illustrating the skin disposition of cannabidiol (n=3) and ALLOOlOl (n=3) in propylene glycol, wherein "n" is the number of skin samples tested.

[0025] Figure 4 is a bar graph illustrating the skin disposition of cannabidiol (n=4) and ALL00102 (n=4) in propylene glycol, wherein "n" is the number of skin samples tested.

[0026] Figure 5 is a graph illustrating the permeation profile of CBD (n=3), ALL00131 (n=3), ALL00132 (n=2), and ALL00140 (n=3), wherein "n" is the number of skin samples tested.

[0027] Figure 6 is a graph illustrating the permeation profile of CBD (n=3), ALL00105 (n=l), ALL00145 (n=3) and ALL00147 (n=2) in 90:8:2 PG:H2O:IPM donor solution with 40% aqueous PEG 400 receiver fluid, wherein "n" is the number of skin samples tested.

[0028] Figure 7 is a graph illustrating the permeation profile of CBD (n=2), ALLOOlOl (n=2), ALL00146 (n=2) and ALL00148 (n=3) in gel formulation with 40% aqueous PEG 400 receiver fluid, wherein "n" is the number of skin samples tested.

[0029] Figure 8 is a bar graph illustrating the skin disposition of CBD (n=3), ALL00131 (n=3), ALL00132 (n=2), and ALL00140 (n=3) in 90:8:2 PG:H2O:IPM donor solution with 60/40 Hanks'/PEG 400 receiver fluid, wherein "n" is the number of skin samples tested.

[0030] Figure 9 is a bar graph illustrating the skin disposition of CBD (n=3), ALLOO 137 (n=3), ALL00142 (n=3), and ALL00143 (n=3) in 90:8:2 PG:H20:IPM donor solution with 40% aqueous PEG 400 receiver fluid, wherein "n" is the number of skin samples tested.

[0031] Figure 10 is a bar graph illustrating the skin disposition of CBD (n=3), ALL00105 (n=3), ALL00145 (n=3), and ALL00147 (n=2) in 90:8:2 PG:H2O:IPM donor solution with 40% aqueous PEG 400 receiver fluid, wherein "n" is the number of skin samples tested.

[0032] Figure 11 is a bar graph illustrating the skin disposition of CBD (n=2), ALLOOlOl (n=2), ALL00146 (n=2), and ALL00148 (n=3) in gel formulation with 40% aqueous PEG 400 receiver fluid, wherein "n" is the number of skin samples tested.

[0033] Figure 12 is a graph illustrating the permeation profile of CBD (n=2), ALL00146 (n=2), and ALLOO 150 (n=3) in gel formulation with 40% aqueous PEG 400 receiver fluid, wherein "n" is the number of skin samples tested.

[0034] Figure 13 is a bar graph illustrating the skin disposition of CBD (n=2), ALLOO 146 (n=3), and ALL00150 (n=3) in gel formulation with 40% aqueous PEG 400 receiver fluid, wherein "n" is the number of skin samples tested.

[0035] Figure 14 is a bar graph illustrating the skin disposition of CBD (n=2) and ALLOO 150 (n=3) in an anhydrous gel formulation with 40% aqueous PEG 400 receiver fluid, wherein "n" is the number of skin samples tested.

DESCRIPTION

[0036] While the present invention is capable of being embodied in various forms, the description below of several embodiments is made with the understanding that the present disclosure is to be considered as an exemplification of the claimed subject matter, and is not intended to limit the appended claims to the specific embodiments illustrated. The headings used throughout this disclosure are provided for convenience only and are not to be construed to limit the claims in any way. Embodiments illustrated under any heading may be combined with embodiments illustrated under any other heading.

[0037] Compounds described herein include pharmaceutically acceptable prodrugs of cannabidiol. One embodiment described herein includes pharmaceutically acceptable prodrugs of cannabidiol which are suitable for non-oral administration and are metabolized to cannabidiol.

A further embodiment described herein includes pharmaceutically acceptable prodrugs of cannabidiol which are suitable for transdermal administration and are metabolized to cannabidiol. The pharmaceutically acceptable prodrugs of cannabidiol may be in any suitable form for administration to a mammal such as in the form of a free base, free acid, salt, hydrate, anhydrate, enantiomer, isomer, tautomer, polymorph, derivative, or the like, provided that the free base, salt, hydrate, enantiomer, isomer, tautomer, or any other pharmacologically suitable derivative is therapeutically active or undergoes conversion within or outside of the body to a therapeutically active form of cannabidiol.

[0038] Compositions described herein comprise at least one pharmaceutically acceptable prodrug of cannabidiol and are suitable for transdermal, oral, buccal, sublingual, injectable, topical, follicular, nasal, ocular, rectal or vaginal administration. The compositions described herein optionally include a vehicle or carrier for the administration of a prodrug of cannabidiol as well as optionally including pharmaceutically acceptable excipients such as solvents, thickening agents, penetration enhancers, wetting agents, lubricants, emollients, binders, taste enhancers, disintegrates, substances added to mask or counteract a disagreeable odor, fragrances or tastes, and substances added to improve appearance or texture of the composition.

[0039] The term prodrug as used herein refers to a compound that undergoes a chemical conversion, through a metabolic process or otherwise within the body of the mammal receiving the compound, into its active form that has medical effects.

[0040] In one embodiment, illustrative cannabidiol prodrugs include those compounds of Formula (I):

(D wherein

R) and R ₂ can be the same or different and are each independently comprised of a hydrogen and/or a bio-labile linker (e.g. ester, oxygenated ester, oxaester, pegylated ester, hydroxylated ester, alkyl ester, amino ester, alkylamino ester, dialkylamino ester, carbonate, alkyl carbonate, carbamate, alkyl carbamate, amino carbamate, alkylamino carbamate, dialkylamino carbamate, or other suitable bio-labile linking structure) and further comprising moieties which can be selected in order to control the rate and extent of absorption and metabolism, including transdermal absorption and metabolism. However, Ri and R ₂ cannot both be a hydrogen atom. Several options for Ri and R ₂ are disclosed herein. Also included herein is the free base, salt, ester, hydrate, amide, enantiomer, isomer, tautomer, polymorph, or derivative thereof of compounds of Formula I.

[0041] Additional embodiments contemplated by the present disclosure include, but are not limited to, those described in WO2007044215, WO2007035945, US2007066657, WO2007026215, WO2007020502, WO2007017264, WO2007009720, US2007004772, US2006287324, US2006287323, US2006287342, US2006287341, US2006089378, US2006079556, US2005143441, US7109216, US2004235854, US2005267161, US2005054659, US2007099990, US2006122229, US2006122230, US2004077650, US6974810, US2004248944, US6977266 and US2006052411 and United States Pat. App. Nos. 10/032,163.

[0042] "Pharmaceutically acceptable salts," or "salts," include the salt of a cannabidiol prodrug suitable for administration to a mammal and includes those prepared from formic, acetic, propionic, succinic, glycolic, gluconic, lactic, malic, tartaric, citric, ascorbic, glucuronic, maleic, fumaric, pyruvic, aspartic, glutamic, benzoic, anthranilic, mesylic, stearic, salicylic, p- hydroxybenzoic, phenylacetic, mandelic, embonic, methanesulfonic, ethanesulfonic, benzenesulfonic, pantothenic, toluenesulfonic, 2-hydroxyethanesulfonic, sulfanilic, cyclohexylaminosulfonic, algenic, beta-hydroxybutyric, galactaric and galacturonic acids. The following list of pharmaceutically acceptable salts is not meant to be exhaustive but merely illustrative as person of ordinary skill in the art would appreciate that other pharmaceutically acceptable salts of cannabidiol and prodrugs of cannabidiol may be prepared.

[0043] In one embodiment, acid addition salts are prepared from the free base forms using conventional methodology involving reaction of the free base with a suitable acid. Suitable acids for preparing acid addition salts include both organic acids, e.g., acetic acid, propionic acid,

glycolic acid, pyruvic acid, oxalic acid, malic acid, malonic acid, succinic acid, maleic acid, fumaric acid, tartaric acid, citric acid, benzoic acid, cinnamic acid, mandelic acid, methanesulfonic acid, ethanesulfonic acid, p-toluenesulfonic acid, salicylic acid, and the like, as well as inorganic acids, e.g., hydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid, phosphoric acid, and the like. The following list of organic and inorganic acids is not meant to be exhaustive but merely illustrative as person of ordinary skill in the art would appreciate that other acids may be used to create pharmaceutically acceptable salts of cannabidiol and prodrugs of cannabidiol. In other embodiments, an acid addition salt is reconverted to the free base by treatment with a suitable base. In still other embodiments, the basic salts are alkali metal salts, e.g., sodium salt.

[0044] In one embodiment, Ri or R ₂ is an ester. In a further embodiment both Ri and R ₂ are esters which can be the same or different. The preparation of CBD esters involves functionalizing the hydroxyl groups that are present within the molecular structure of cannabidiol. hi another embodiment, the esters of either or both of Ri and/or R ₂ are oxygenated. In another embodiment, either or both of Ri and/or R ₂ are oxygenated esters which are oxaesters. hi another embodiment, either or both of Rj and/or R ₂ are oxaesters which are pegylated. hi further embodiments, either or both of Rj and/or R ₂ are pegylated oxaesters can independently have 1 ethylene glycol repeat unit, 2 ethylene glycol repeat units, 3 ethylene glycol repeat units, 4 ethylene glycol repeat units, 5 ethylene glycol repeat units, 6 ethylene glycol repeat units, 7 ethylene glycol repeat units, 8 ethylene glycol repeat units, 9 ethylene glycol repeat units, 10 ethylene glycol repeat units, 11 ethylene glycol repeat units, 12 ethylene glycol repeat units, 13 ethylene glycol repeat units, 14 ethylene glycol repeat units and 15 ethylene glycol repeat units. In a further embodiment, either or both of Ri and/or R ₂ are esters which are hydroxylated. hi a further embodiment, either or both of Ri and/or R ₂ are esters which are alkyl ester, hi additional embodiments, either or both of Ri and/or R ₂ are alkyl esters independently having 1 alkyl carbon, 2 alkyl carbons, 3 alkyl carbons, 4 alkyl carbons, 5 alkyl carbons, 6 alkyl carbons, 7 alkyl carbons, 8 alkyl carbons, 9 alkyl carbons, 10 alkyl carbons, 11 alkyl carbons, 12 alkyl carbons, 13 alkyl carbons, 14 alkyl carbons and 15 alkyl carbons, hi other embodiments, either or both of Ri and/or R ₂ are esters which are amino esters independently having 1 amino group, 2 amino groups, 3 amino groups, 4 amino groups and 5 amino groups, hi another embodiment, either or both of Ri and/or R ₂ are amino esters which are alkylamino ester, hi a further

embodiment, either or both of Ri and/or R ₂ are alkylamino esters independently having 1 amino group, 2 amino groups, 3 amino groups, 4 amino groups and 5 amino groups and independently having 1 alkyl carbon, 2 alkyl carbons, 3 alkyl carbons, 4 alkyl carbons, 5 alkyl carbons, 6 alkyl carbons, 7 alkyl carbons, 8 alkyl carbons, 9 alkyl carbons, 10 alkyl carbons, 11 alkyl carbons, 12 alkyl carbons, 13 alkyl carbons, 14 alkyl carbons and 15 alkyl carbons. In another embodiment the ester is a glycolic acid ester. In another embodiment the ester is a glycolic acid ester, a hyluronic ester or a lactic acid ester. Esters can be reconverted to the free acids, if desired, by using conventional procedures such as hydrogenolysis or hydrolysis.

[0045] In one embodiment, Ri or R ₂ is a carbamate. In a further embodiment both Ri and R ₂ are carbamates which can be the same or different. The preparation of CBD carbamates involves functionalizing the hydroxyl groups that are present within the molecular structure of cannabidiol. In another embodiment, the carbamates of either or both of Ri and/or R ₂ are oxygenated. In another embodiment, either or both of Ri and/or R ₂ are oxygenated carbamates which are oxacarbamates. In another embodiment, either or both of Ri and/or R ₂ are oxacarbamates which are pegylated. In further embodiments, either or both of Ri and/or R ₂ are pegylated oxacarbamates can independently have 1 ethylene glycol repeat unit, 2 ethylene glycol repeat units, 3 ethylene glycol repeat units, 4 ethylene glycol repeat units, 5 ethylene glycol repeat units, 6 ethylene glycol repeat units, 7 ethylene glycol repeat units, 8 ethylene glycol repeat units, 9 ethylene glycol repeat units, 10 ethylene glycol repeat units, 11 ethylene glycol repeat units, 12 ethylene glycol repeat units, 13 ethylene glycol repeat units, 14 ethylene glycol repeat units and 15 ethylene glycol repeat units. In a further embodiment, either or both of Ri and/or R ₂ are carbamates which are hydroxylated. In a further embodiment, either or both of Ri and/or R ₂ are carbamates which are alkyl carbamates. In additional embodiments, either or both of R i and/or R ₂ are alkyl carbamates independently having 1 alkyl carbon, 2 alkyl carbons, 3 alkyl carbons, 4 alkyl carbons, 5 alkyl carbons, 6 alkyl carbons, 7 alkyl carbons, 8 alkyl carbons, 9 alkyl carbons, 10 alkyl carbons, 11 alkyl carbons, 12 alkyl carbons, 13 alkyl carbons, 14 alkyl carbons and 15 alkyl carbons. In other embodiments, either or both of Ri and/or R ₂ are carbamates which are amino carbamates independently having 1 amino group, 2 amino groups, 3 amino groups, 4 amino groups and 5 amino groups. In another embodiment, either or both of Ri and/or R ₂ are amino carbamates which are alkylamino carbamates. In a further embodiment, either or both of Ri and/or R ₂ are alkylamino carbamates independently having 1 amino group, 2

amino groups, 3 amino groups, 4 amino groups and 5 amino groups and independently having 1 alkyl carbon, 2 alkyl carbons, 3 alkyl carbons, 4 alkyl carbons, 5 alkyl carbons, 6 alkyl carbons, 7 alkyl carbons, 8 alkyl carbons, 9 alkyl carbons, 10 alkyl carbons, 11 alkyl carbons, 12 alkyl carbons, 13 alkyl carbons, 14 alkyl carbons and 15 alkyl carbons. In another embodiment the carbamate is a glycolic acid carbamate, a hyluronic carbamate or a lactic acid carbamate. Carbamates can be reconverted to the free acids, if desired, by using conventional procedures such as hydrogenolysis or hydrolysis.

[0046] In one embodiment, R] or R ₂ is a carbonate. In a further embodiment both Ri and R ₂ are carbonates which can be the same or different. The preparation of CBD carbonates involves functionalizing the hydroxyl groups that are present within the molecular structure of cannabidiol. In another embodiment, the carbonates of either or both of Ri and/or R ₂ are oxygenated. In another embodiment, either or both of Ri and/or R ₂ are oxygenated carbonates which are oxacarbonates. In another embodiment, either or both of Ri and/or R ₂ are oxacarbonates which are pegylated. In further embodiments, either or both of Ri and/or R ₂ are pegylated oxacarbonates can independently have 1 ethylene glycol repeat unit, 2 ethylene glycol repeat units, 3 ethylene glycol repeat units, 4 ethylene glycol repeat units, 5 ethylene glycol repeat units, 6 ethylene glycol repeat units, 7 ethylene glycol repeat units, 8 ethylene glycol repeat units, 9 ethylene glycol repeat units, 10 ethylene glycol repeat units, 11 ethylene glycol repeat units, 12 ethylene glycol repeat units, 13 ethylene glycol repeat units, 14 ethylene glycol repeat units and 15 ethylene glycol repeat units. In a further embodiment, either or both of Ri and/or R ₂ are carbonates which are hydroxylated. In a further embodiment, either or both of Ri and/or R ₂ are carbonates which are alkyl carbonates. In additional embodiments, either or both of R _J and/or R ₂ are alkyl carbonates independently having 1 alkyl carbon, 2 alkyl carbons, 3 alkyl carbons, 4 alkyl carbons, 5 alkyl carbons, 6 alkyl carbons, 7 alkyl carbons, 8 alkyl carbons, 9 alkyl carbons, 10 alkyl carbons, 11 alkyl carbons, 12 alkyl carbons, 13 alkyl carbons, 14 alkyl carbons and 15 alkyl carbons. In other embodiments, either or both of Ri and/or R ₂ are carbonates which are amino carbonates independently having 1 amino group, 2 amino groups, 3 amino groups, 4 amino groups and 5 amino groups. In another embodiment, either or both of Ri and/or R ₂ are amino carbonates which are alkylamino carbonates. In a further embodiment, either or both of Ri and/or R ₂ are alkylamino carbonates independently having 1 amino group, 2 amino groups, 3 amino groups, 4 amino groups and 5 amino groups and independently having 1

alkyl carbon, 2 alkyl carbons, 3 alkyl carbons, 4 alkyl carbons, 5 alkyl carbons, 6 alkyl carbons, 7 alkyl carbons, 8 alkyl carbons, 9 alkyl carbons, 10 alkyl carbons, 11 alkyl carbons, 12 alkyl carbons, 13 alkyl carbons, 14 alkyl carbons and 15 alkyl carbons. In another embodiment the carbonate is a glycolic acid carbonate, a hyluronic carbonate or a lactic acid carbonate. Carbonates can be reconverted to the free acids, if desired, by using conventional procedures such as hydrogenolysis or hydrolysis.

[0047] Further embodiments described herein are pharmaceutical compositions comprising: (a) a cannabidiol prodrug selected from the group consisting of:

wherein Ri and R ₂ are independently selected from hydrogen, ester, oxygenated ester, oxaester, pegylated ester, hydroxylated ester, alkyl ester, amino ester, alkylamino ester, dialkylamino ester, glycolic acid ester, hyaluronic acid ester, lactic acid ester, carbonate, oxygenated carbonate, oxacarbonate, pegylated carbonate, hydroxylated carbonate, alkyl carbonate, amino carbonate, alkylamino carbonate, dialkylamino carbonate, glycolic acid carbonate, hyaluronic acid carbonate, lactic acid carbonate, carbamate, oxygenated carbamate, oxacarbamate, pegylated carbamate, hydroxylated carbamate, alkyl carbamate, amino carbamate, alkylamino carbamate, dialkylamino carbamate, glycolic acid carbamate, hyaluronic acid carbamate and lactic acid carbamate; and wherein R ₁ and R ₂ can not both be hydrogen; and

(b) a pharmaceutical excipient.

[0048] A method of administering a compound to a mammal comprising the steps of: (a) combining a compound selected from the group consisting of:

wherein Ri and R ₂ are independently selected from hydrogen, ester, oxygenated ester, oxaester, pegylated ester, hydroxylated ester, alkyl ester, amino ester, alkylamino ester, dialkylamino ester, glycolic acid ester, hyaluronic acid ester, lactic acid ester, carbonate, oxygenated carbonate, oxacarbonate, pegylated carbonate, hydroxylated carbonate, alkyl carbonate, amino carbonate, alkylamino carbonate, dialkylamino carbonate, glycolic acid carbonate, hyaluronic acid carbonate, lactic acid carbonate, carbamate, oxygenated carbamate, oxacarbamate, pegylated carbamate, hydroxylated carbamate, alkyl carbamate, amino carbamate, alkylamino carbamate, dialkylamino carbamate, glycolic acid carbamate, hyaluronic acid carbamate and lactic acid carbamate; and wherein R| and R ₂ can not both be hydrogen; with a pharmaceutical excipient to form a pharmaceutical composition;

(b) creating a dosage form suitable for administration to a mammal from the pharmaceutical composition; and

(c) administering the dosage form to a mammal.

[0049] Additional embodiments include methods of transdermally delivering a cannabidiol to a mammal comprising the steps of:

(a) selecting a cannabidiol prodrug from the group consisting of:

wherein Ri and R ₂ are independently selected from hydrogen, ester, oxygenated ester, oxaester, pegylated ester, hydroxylated ester, alkyl ester, amino ester, alkylamino ester, dialkylamino ester, glycolic acid ester, hyaluronic acid ester, lactic acid ester, carbonate, oxygenated carbonate, oxacarbonate, pegylated carbonate, hydroxylated carbonate, alkyl carbonate, amino carbonate, alkylamino carbonate, dialkylamino carbonate, glycolic acid carbonate, hyaluronic acid carbonate, lactic acid carbonate, carbamate, oxygenated carbamate, oxacarbamate, pegylated carbamate, hydroxylated carbamate, alkyl carbamate, amino carbamate, alkylamino carbamate, dialkylamino carbamate, glycolic acid carbamate, hyaluronic acid carbamate and lactic acid carbamate; and wherein Ri and R ₂ can not both be hydrogen.

(b) combining the selected cannabidiol prodrug with a pharmaceutically acceptable excipient to form a pharmaceutical composition; and

(c) contacting the pharmaceutical composition with the skin of a mammal.

[0050] A further embodiment described herein is a method of treating a medical condition in a mammal comprising the steps of administering a cannabidiol prodrug selected from the group consisting of:

wherein Ri and R ₂ are independently selected from hydrogen, ester, oxygenated ester, oxaester, pegylated ester, hydroxylated ester, alkyl ester, amino ester, alkylamino ester, dialkylamino ester, glycolic acid ester, hyaluronic acid ester, lactic acid ester, carbonate, oxygenated carbonate, oxacarbonate, pegylated carbonate, hydroxylated carbonate, alkyl carbonate, amino carbonate, alkylamino carbonate, dialkylamino carbonate, glycolic acid carbonate, hyaluronic acid carbonate, lactic acid carbonate, carbamate, oxygenated carbamate, oxacarbamate, pegylated carbamate, hydroxylated carbamate, alkyl carbamate, amino carbamate, alkylamino carbamate, dialkylamino carbamate, glycolic acid carbamate, hyaluronic acid carbamate and lactic acid carbamate; wherein Ri and R ₂ can not both be hydrogen; and wherein the medical condition is selected from the group consisting of: nausea, emesis, pain, wasting syndrome, HIV-wasting, chemotherapy induced nausea and vomiting, alcohol use disorders, dystonia, multiple sclerosis, inflammatory bowel disorders, arthritis, dermatitis, Rheumatoid arthritis, systemic lupus erythematosus, anti-inflammatory, anti-convulsant, antipsychotic, anti-oxidant, neuroprotective, anti-cancer, immunomodulatory effects, peripheral neuropathic pain, neuropathic pain associated with post-herpetic neuralgia, diabetic neuropathy, shingles, burns, actinic keratosis, oral cavity sores and ulcers, post-episiotomy pain, psoriasis, pruritis, contact dermatitis, eczema, bullous dermatitis herpetiformis, exfoliative dermatitis, mycosis fungoides, pemphigus, severe erythema multiforme (e.g., Stevens-Johnson syndrome), seborrheic dermatitis, ankylosing spondylitis, psoriatic arthritis, Reiter's syndrome, gout, chondrocalcinosis, joint pain secondary to dysmenorrhea, fibromyalgia, musculoskeletal pain, neuropathic-postoperative complications, polymyositis, acute nonspecific tenosynovitis, bursitis, epicondylitis, post-traumatic osteoarthritis, synovitis, juvenile rheumatoid arthritis and inhibition

of hair growth.

[0051] In one embodiment, the resulting prodrug is more hydrophilic than cannabidiol and therefore more water soluble. The logio values of the water/octanol partition coefficient (log P) for cannabidiol and various prodrugs of cannabidiol are shown in Table 15. A further embodiment is a prodrug of cannabidiol having a log P value less than that of cannabidiol. A further embodiment is a prodrug of cannabidiol having a log P value greater than that of cannabidiol. A further embodiment is a prodrug of cannabidiol having a log P value which is approximately equal to that of cannabidiol.

[0052] Pharmaceutical Excipients

[0053] The pharmaceutical compositions described herein can, if desired, include one or more pharmaceutically acceptable excipients. The term "excipient" herein means any substance, not itself a therapeutic agent, used as a carrier or vehicle for delivery of a therapeutic agent to a subject or combined with a therapeutic agent (e.g., to create a pharmaceutical composition) to improve its handling or storage properties or to permit or facilitate formation of a dose unit of the composition. Excipients include, by way of illustration and not limitation, binders, disintegrants, taste enhancers, solvents, thickening agents, penetration enhancers, wetting agents, lubricants, emollients, substances added to mask or counteract a disagreeable odor, fragrances or taste, and substances added to improve appearance or texture of the composition. Any such excipients can be used in any dosage forms according to the present disclosure. The foregoing classes of excipients are not meant to be exhaustive but merely illustrative as a person of ordinary skill in the art would recognize that additional types of excipients could be used to achieve the desired goals for delivery of the cannabidiol prodrug.

[0054] Compositions of the disclosure containing excipients can be prepared by any technique known to a person of ordinary skill in the art of pharmacy, pharmaceutics, drug delivery, pharmacokinetics, medicine or other related discipline that comprises admixing an excipient with a drug or therapeutic agent.

[0055] In one embodiment, the cannabidiol prodrugs described herein can be combined with a penetration enhancing agent for transdermal or topical delivery. Non-limiting examples of penetration enhancing agents include C8-C22 fatty acids such as isostearic acid, octanoic

acid, and oleic acid; C8-C22 fatty alcohols such as oleyl alcohol and lauryl alcohol; lower alkyl esters of C8-C22 fatty acids such as ethyl oleate, isopropyl myristate, butyl stearate, and methyl laurate; di(lower)alkyl esters of C6-C22 diacids such as diisopropyl adipate; monoglycerides of C8-C22 fatty acids such as glyceryl monolaurate; tetrahydrofurfuryl alcohol polyethylene glycol ether; polyethylene glycol, propylene glycol; 2-(2-ethoxyethoxy)ethanol; diethylene glycol monomethyl ether; alkylaryl ethers of polyethylene oxide; polyethylene oxide monomethyl ethers; polyethylene oxide dimethyl ethers; dimethyl sulfoxide; glycerol; ethyl acetate; acetoacetic ester; N-alkylpyrrolidone; and terpenes. Additional penetration enhancers suitable for use can also be found in United States Pat. App. No. 10/032,163.

[0056] In one embodiment, the cannabidiol prodrugs can be combined with a thickening or gelling agent. Non-limiting examples of thickening agents (aka gelling agents) which ma be used herein include anionic polymers such as polyacrylic acid (CARBOPOL® by Noveon, Inc., Cleveland, Ohio), carboxypolymethylene, carboxymethylcellulose and the like, including derivatives of Carbopol® polymers, such as Carbopol® Ultrez 10, Carbopol® 940, Carbopol® 941, Carbopol® 954, Carbopol® 980, Carbopol® 981, Carbopol® ETD 2001, Carbopol® EZ-2 and Carbopol® EZ-3, and other polymers such as Pemulen® polymeric emulsifiers, and Noveon® polycarbophils. Additional thickening agents, enhancers and adjuvants may generally be found in Remington's The Science and Practice of Pharmacy as well as the Handbook f Pharmaceutical Excipients, Arthur H. Kibbe ed. 2000. Thickening agents or gelling agents are present in an amount sufficient to provide the desired rheological properties of the composition. Illustratively, one or more pharmaceutically acceptable thickening agent or gelling agent are present in a total amount by weight of about 0.1%, about 0.25%, about 0.5%, about 0.75%, about 1%, about 1.25%, about 1.5%, about 1.75%, about 2.0%, about 2.25%, about 2.5%, about 2.75%, about 3.0%, about 3.25%, about 3.5%, about 3.75%, about 4.0%, about 4.25%, about 4.5%, about 4.75%, about 5.0%, about 5.25%, about 5.5%, about 5.75%, about 6.0%, about 6.25%, about 6.5%, about 6.75%, about 7.0%, about 7.25%, about 7.5%, about 7.75%, about 8.0%, about 8.25%, about 8.5%, about 8.75%, about 9.0%, about 9.25%, about 9.5%, about 9.75%, about 10%, about 11%, about 11.5%, about 12%, about 12.5%, about 13%, about 13.5%, about 14%, about 14.5% or about 15%.

[0057] In one embodiment a neutralizing agent is optionally present to assist in forming a gel. Suitable neutralizing agents include sodium hydroxide (e.g., as an aqueous mixture),

potassium hydroxide (e.g., as an aqueous mixture), ammonium hydroxide (e.g., as an aqueous mixture), triethanolamine, tromethamine (2-amino 2-hydroxymethyl-l, 3 propanediol), aminomethyl propanol (AMP), tetrahydroxypropyl ethylene diamine, diisopropanolamine, Ethomeen C-25 (Armac Industrial Division), Di-2 (ethylhexyl) amine (BASF-Wyandotte Corp., Intermediate Chemicals Division), triamylamine, Jeffamine D- 1000 (Jefferson Chemical Co.), b- Dimethylaminopropionitrite (American Cyanamid Co.), Armeen CD (Armac Industrial Division), Alamine 7D (Henkel Corporation), dodecylamine and morpholine. The neutralizing agent is present in an amount sufficient to form a gel which is suitable for contact with the skin of a mammal.

[0058] In one embodiment, the formulation is a gel, an ointment, a cream or a patch and comprises a cannabidiol prodrug, optionally a penetration enhancing agent, a thickening agent, a lower alcohol, such as ethanol or isopropanol; and water. In another embodiment, the formulation is a gel, an ointment, a cream or a patch, further comprised of an aqueous solution of sodium hydroxide or triethanolamine or an aqueous solution of potassium hydroxide, or a combination thereof, in an amount sufficient, as is known in the art, to assist the gelling agent in forming a gel.

[0059] In one embodiment, a solution of sodium hydroxide is used, such as, e.g., 0.1 N sodium hydroxide solution, 0.2 N sodium hydroxide solution, 0.5 N sodium hydroxide solution, 1.0 N sodium hydroxide solution, 1.5 N sodium hydroxide solution, 2.0 N sodium hydroxide solution, or any other suitable solution for providing an amount sufficient of the aqueous sodium hydroxide to form the desired gel. In one embodiment, the composition results from combining a gelling agent with a neutralizing agent such as about 1% to about 10% (wt/wt) 0.1 N sodium hydroxide. Of course, other suitable neutralizing agents can be used as can other concentrations of aqueous sodium hydroxide so long as there is a sufficient amount of OH ^" ions to assist in the formation of a gel.

[0060] Additional embodiments include the following compositions:

[0061] Gel formulation used with patches (18 mg/niL CBD or cannabidiol prodrug)

75.2% propylene glycol, USP

18.8% sterile water for injection, USP

6.0% diethylene glycol monoethyl ether (Transcutol HP), EP/USP/NF

5.0% hydroxyethylcellulose (Natrosol®), NF based on weight of other three components

[0062] Gel formulation used for rubbing into skin

72.5-67.5% absolute ethanol, USP/NF 20.38-15.38% sterile water for injection, USP

4 .72% 0.1 N NaOH (NF) in sterile water for injection, USP

1-10% cannabidiol or cannabidiol prodrug

0, .9% Carbopol 980®, NF

0, .5% isopropyl myristate, USP/NF

[0063] Gel formulation

78.1% absolute ethanol, USP/NF

15.3% sterile water for injection, USP

1.5% triethanolamine, NF

3.5% cannabidiol or cannabidiol prodrug

1.0% Carbopol 980®, NF

0.6% isopropyl myristate, USP/NF

[0064] Gel formulation

91.75-82.75% absolute ethanol, USP/NF

5.0% propylene glycol, USP

1-10% cannabidiol or cannabidiol prodrug

1.25% polyoxyethylene (15) cocoalkylamines (Ethomeen® C/25)

0.5% Carbopol 980®, NF

0.5% isopropyl myristate, USP/NF

[0065] Compositions described herein optionally comprise one or more pharmaceutically acceptable wetting agents as excipients. Non-limiting examples of surfactants that can be used as wetting agents in compositions of the disclosure include quaternary ammonium compounds, for example benzalkonium chloride, benzethonium chloride and cetylpyridinium chloride, dioctyl sodium sulfosuccinate, polyoxyethylene alkylphenyl ethers, for example nonoxynol 9, nonoxynol 10, and octoxynol 9, poloxamers (polyoxyethylene and polyoxypropylene block copolymers), polyoxyethylene fatty acid glycerides and oils, for example polyoxyethylene (8) caprylic/capric mono- and diglycerides (e.g., Labrasol™ of Gattefosse), polyoxyethylene (35) castor oil and polyoxyethylene (40) hydrogenated castor oil; polyoxyethylene alkyl ethers, for example polyoxyethylene (20) cetostearyl ether, polyoxyethylene fatty acid esters, for example polyoxyethylene (40) stearate, polyoxyethylene sorbitan esters, for example polysorbate 20 and

polysorbate 80 (e.g., Tween™ 80 of ICI), propylene glycol fatty acid esters, for example propylene glycol laurate (e.g., Lauroglycol ^rM of Gattefosse), sodium lauryl sulfate, fatty acids and salts thereof, for example oleic acid, sodium oleate and triethanolamine oleate, glyceryl fatty acid esters, for example glyceryl monostearate, sorbitan esters, for example sorbitan monolaurate, sorbitan monooleate, sorbitan monopalmitate and sorbitan monostearate, tyloxapol, and mixtures thereof. Such wetting agents, if present, constitute in total about 0.25% to about 15%, about 0.4% to about 10%, or about 0.5% to about 5%, of the total weight of the composition. Illustratively, one or more pharmaceutically acceptable wetting agents are present in a total amount by weight of about 0.25%, about 0.5%, about 0.75%, about 1%, about 1.25%, about 1.5%, about 1.75%, about 2.0%, about 2.25%, about 2.5%, about 2.75%, about 3.0%, about 3.25%, about 3.5%, about 3.75%, about 4.0%, about 4.25%, about 4.5%, about 4.75%, about 5.0%, about 5.25%, about 5.5%, about 5.75%, about 6.0%, about 6.25%, about 6.5%, about 6.75%, about 7.0%, about 7.25%, about 7.5%, about 7.75%, about 8.0%, about 8.25%, about 8.5%, about 8.75%, about 9.0%, about 9.25%, about 9.5%, about 9.75% or about 10%.

[0066] Compositions described herein optionally comprise one or more pharmaceutically acceptable lubricants (including anti-adherents and/or glidants) as excipients. Suitable lubricants include, either individually or in combination, glyceryl behapate (e.g., Compritol™ 888); stearic acid and salts thereof, including magnesium (magnesium stearate), calcium and sodium stearates; hydrogenated vegetable oils (e.g., Sterotex™); colloidal silica; talc; waxes; boric acid; sodium benzoate; sodium acetate; sodium fumarate; sodium chloride; DL-leucine; PEG (e.g., Carbowax™ 4000 and Carbowax™ 6000); sodium oleate; sodium lauryl sulfate; and magnesium lauryl sulfate. Such lubricants, if present, constitute in total about 0.1% to about 10%, about 0.2% to about 8%, or about 0.25% to about 5%, of the total weight of the composition. Illustratively, one or more pharmaceutically acceptable lubricants are present in a total amount by weight of about 0.1%, about 0.2%, about 0.3%, about 0.4%, about 0.5%, about 0.6%, about 0.7%, about 0.8%, about 0.9%, about 1.0%, about 1.1%, about 1.2%, about 1.3%, about 1.4%, about 1.5%, about 1.6%, about 1.7%, about 1.8%, about 1.9%, about 2.0%, about 2.1%, about 2.2%, about 2.3%, about 2.4%, about 2.5%, about 2.6%, about 2.7%, about 2.8%, about 2.9%, about 3.0%, about 3.1%, about 3.2%, about 3.3%, about 3.4%, about 3.5%, about 3.6%, about 3.7%, about 3.8%, about 3.9%, about 4.0%, about 4.1%, about 4.2%, about 4.3%, about 4.4%, about 4.5%, about 4.6%, about 4.7%, about 4.8%, about 4.9%, about 5.0%, about 5.1%, about

5.2%, about 5.3%, about 5.4%, about 5.5%, about 5.6%, about 5.7%, about 5.8%, about 5.9%, about 6.0%, about 6.1%, about 6.2%, about 6.3%, about 6.4%, about 6.5%, about 6.6%, about 6.7%, about 6.8%, about 6.9%, about 7.0%, about 7.1%, about 7.2%, about 7.3%, about 7.4%, about 7.5%, about 7.6%, about 7.7%, about 7.8%, about 7.9%, about 8.0%, about 8.1%, about 8.2%, about 8.3%, about 8.4%, about 8.5%, about 8.6%, about 8.7%, about 8.8%, about 8.9%, about 9.0%, about 9.1%, about 9.2%, about 9.3%, about 9.4%, about 9.5%, about 9.6%, about 9.7%, about 9.8%, about 9.9% or about 10.0%.

[0067] In another embodiment, the compositions described herein optionally comprise an emollient. Illustrative emollients include mineral oil, mixtures of mineral oil and lanolin alcohols, cetyl alcohol, cetostearyl alcohol, petrolatum, petrolatum and lanolin alcohols, cetyl esters wax, cholesterol, glycerin, glyceryl monostearate, isopropyl myristate, isopropyl palmitate, lecithin, allyl caproate, althea officinalis extract, arachidyl alcohol, argobase EUC, butylene glycol dicaprylate/dicaprate, acacia, allantoin, carrageenan, cetyl dimethicone, cyclomethicone, diethyl succinate, dihydroabietyl behenate, dioctyl adipate, ethyl laurate, ethyl palmitate, ethyl stearate, isoamyl laurate, octanoate, PEG-75 lanolin, sorbitan laurate, walnut oil, wheat germ oil, super refined almond, super refined sesame, super refined soyabean, octyl palmitate, caprylic/capric triglyceride and glyceryl cocoate. An emollient, if present, is present in the compositions described herein in an amount of about 1% to about 30%, about 3% to about 25%, or about 5% to about 15%, by weight. Illustratively, one or more emollients are present in a total amount of about 1%, about 2%, about 3%, about 4%, about 5%, about 6%, about 7%, about 8%, about 9%, about 10%, about 11%, about 12%, about 13%, about 14%, about 15%, about 16%, about 17%, about 18%, about 19%, about 20%, about 21%, about 22%, about 23%, about 24%, about 25%, about 26%, about 27%, about 28%, about 29%, or about 30%, by weight.

[0068] In one embodiment, the compositions described herein comprise an antimicrobial preservative. Illustrative anti-microbial preservatives include acids, including but not limited to benzoic acid, phenolic acid, sorbic acids, alcohols, benzethonium chloride, bronopol, butylparaben, cetrimide, chlorhexidine, chlorobutanol, chlorocresol, cresol, ethylparaben, imidurea, methylparaben, phenol, phenoxyethanol, phenylethyl alcohol, phenylmercuric acetate, phenylmercuric borate, phenylmercuric nitrate, potassium sorbate, propylparaben, sodium propionate, or thimerosal. The anti-microbial preservative, if present, is present in an amount of about 0.1% to about 5%, about 0.2% to about 3%, or about 0.3% to about 2%, by weight, for

example about 0.2%, about 0.4%, about 0.6%, about 0.8%, about 1%, about 1.2%, about 1.4%, about 1.6%, about 1.8%, about 2%, about 2.2%, about 2.4%, about 2.6%, about 2.8%, about 3.0%, about 3.2%, about 3.4%, about 3.6%, about 3.8%, about 4%, about 4.2%, about 4.4%, about 4.6%, about 4.8%, or about 5%.

[0069] Compositions described herein optionally compromise one or more emulsifying agents. The term "emulsifying agent" refers to an agent capable of lowering surface tension between a non-polar and polar phase and includes compounds defined elsewhere as "self emulsifying" agents. Suitable emulsifying agents can come from any class of pharmaceutically acceptable emulsifying agents including carbohydrates, proteins, high molecular weight alcohols, wetting agents, waxes and finely divided solids. The optional emulsifying agent, if present, is present in a composition in a total amount of about 1% to about 15%, about 1% to about 12%, about 1% to about 10%, or about 1% to about 5% by weight of the composition. Illustratively, one or more emulsifying agents are present in a total amount by weight of about 1%, about 2%, about 3%, about 4%, about 5%, about 6%, about 7%, about 8%, about 9%, about 10%, about 11%, about 12%, about 13%, about 14%, or about 15%.

[0070] In another embodiment, the water immiscible solvent comprises propylene glycol, and is present in a composition in an amount of about 1% to about 99%, by weight of the composition, for example about 1%, about 5%, about 10%, about 15%, about 20%, about 25%, about 30%, about 35%, about 40%, about 45%, about 50%, about 55%, about 60%, about 65%, about 70%, about 75%, about 80%, about 85%, about 90%, about 95% or about 99%.

[0071] Compositions described herein may optionally comprise one or more binding agents. Binding agents may be either dry or wet. Dry binding agents may include simple and complex carbohydrates (e.g., sucrose, glucose, fructose, maltose, lactose, maltodextrins, starch, modified starches, mannitol, sorbitol, maltitol, xylitol, and erthritol), cellulose, and cellulosic derivatives (e.g., microcrystalline cellulose, carboxymethyl cellulose, and hydroxyethyl cellulose). Wet binder agents may include, polyvinyl pyrrolidone, methycellulose, hydroxypropyl cellulose, hydroxypropyl methylcellulose, carboxymethylcellulose, xanthan gum, carrageenan gum, locust bean gum, alginates, and acacia. Depending on the desired result, a person of ordinary skill in the art of pharmacy, pharmaceutics, drug delivery, pharmacokinetics, medicine or other related discipline that comprises admixing an excipient with a drug or therapeutic agent to a composition

would be able to select the appropriate binding agent and the relative concentration of the binding agent.

[0072] In another embodiment, the compositions described herein may contain disintegrants, such as sodium starch glycolate, crosspovidone, crosscarmellose, microcrystalline cellulose and starch. Depending on the desired result, a person of ordinary skill in the art of pharmacy, pharmaceutics, drug delivery, pharmacokinetics, medicine or other related discipline that comprises admixing an excipient with a drug or therapeutic agent to a composition would be able to select the appropriate disintegrant and the relative concentration of the disintegrant.

[0073] In a further embodiment, the compositions disclosed herein may contain lubricants, such as magnesium stearate, stearic acid and its pharmaceutically acceptable salts, talc, vegetable oils, and waxes. Depending on the desired result, a person of ordinary skill in the art of pharmacy, pharmaceutics, drug delivery, pharmacokinetics, medicine or other related discipline that comprises admixing an excipient with a drug or therapeutic agent to a composition would be able to select the appropriate lubricant and the relative concentration of the lubricant.

[0074] Compositions described herein may also optionally comprise one or more taste enhancers, such as sweeteners, including aspartame, acesulfame potassium, sucralose and saccharin or taste masking agents, such as flavorings. Depending on the desired result, a person of ordinary skill in the art of pharmacy, pharmaceutics, drug delivery, pharmacokinetics, medicine or other related discipline that comprises admixing an excipient with a drug or therapeutic agent to a composition would be able to select the appropriate taste enhancer or taste making agent and the relative concentration of the taste enhancer or taste masking agent.

[0075] Therapeutic Uses

[0076] In one embodiment, compositions disclosed herein comprise one or more cannabidiol prodrugs in a total amount of between about 0.1% and about 95% by weight of the composition, for example about 0.1%, about 0.2%, about 0.3%, about 0.4%, about 0.5%, about 0.6%, about 0.7%, about 0.8%, about 0.9%, about 1%, about 2%, about 3%, about 4%, about 5%, about 6%, about 7%, about 8%, about 9%, about 10%, about 15%, about 20%, about 25%, about 30%, about 35%, about 40%, about 45%, about 50%, about 55%, about 60%, about 65%, about 70%, about 75%, about 80%, about 85%, about 90% or about 95%.

[0077] The term "therapeutically effective amount" or "therapeutically and/or prophylactically effective amount" as used herein refers to an amount of compound or agent that is sufficient to elicit the required or desired therapeutic and/or prophylactic response, as the particular treatment context may require.

[0078] It will be understood that a therapeutically and/or prophylactically effective amount of a drug for a subject is dependent inter alia on the body weight of the subject as well as other factors known to a person of ordinary skill in the art. A "subject" herein to which a therapeutic agent or composition thereof can be administered includes mammals such as a human subject of either sex and of any age, and also includes any nonhuman animal, particularly a domestic or companion animal, illustratively a cat, dog or a horse as well as laboratory animals such as guinea pigs.

[0079] The terms "treat", "treated", "treating" and "treatment" are to be broadly understood as referring to any response to, or anticipation of, a medical condition in a mammal, particularly a human, and includes but is not limited to:

(i) preventing the medical condition from occurring in a subject, which may or may not be predisposed to the condition, but has not yet been diagnosed with the condition and, accordingly, the treatment constitutes prophylactic treatment for the medical condition;

(ii) inhibiting the medical condition, i.e., arresting, slowing or delaying the onset, development or progression of the medical condition; or

(iii) relieving the medical condition, i.e., causing regression of the medical condition.

[0080] In one embodiment, a therapeutically effective amount of a cannabidiol prodrug is administered to treat a medical condition selected from the group consisting of: nausea, emesis, pain, wasting syndrome, HIV-wasting, chemotherapy induced nausea and vomiting, alcohol use disorders, dystonia, multiple sclerosis, inflammatory bowel disorders, arthritis, dermatitis, Rheumatoid arthritis, systemic lupus erythematosus, anti-inflammatory, anti-convulsant, antipsychotic, anti-oxidant, neuroprotective, anti-cancer, immunomodulatory effects, peripheral neuropathic pain, neuropathic pain associated with post-herpetic neuralgia, diabetic neuropathy, shingles, burns, actinic keratosis, oral cavity sores and ulcers, post-episiotomy pain, psoriasis,

pruritis, contact dermatitis, eczema, bullous dermatitis herpetiformis, exfoliative dermatitis, mycosis fungoides, pemphigus, severe erythema multiforme (e.g., Stevens-Johnson syndrome), seborrheic dermatitis, ankylosing spondylitis, psoriatic arthritis, Reiter's syndrome, gout, chondrocalcinosis, joint pain secondary to dysmenorrhea, fibromyalgia, musculoskeletal pain, neuropathic-postoperative complications, polymyositis, acute nonspecific tenosynovitis, bursitis, epicondylitis, post-traumatic osteoarthritis, synovitis, juvenile rheumatoid arthritis and inhibition of hair growth.

[0081] Pharmaceutical Dosage Forms

[0082] In one embodiment, a single dosage unit of any formulation comprises a therapeutically effective amount or a therapeutically and/or prophylactically effective amount of a cannabidiol prodrug.

[0083] In one embodiment, compositions described herein are suitable for transdermal administration. In another embodiment, transdermally administrable compositions are adapted for administration in and/or around the abdomen, back, chest, legs, arms, scalp or other suitable skin surface and may include formulations in which the cannabidiol prodrug is administered in patches, ointments, creams, suspensions, lotions, pastes, gels, sprays, foams or oils.

[0084] In another embodiment, compositions described herein which are transdermally administrable include formulations in which the cannabidiol prodrug is placed in a glycol or gel formulation.

[0085] In one embodiment, compositions described herein are suitable for topical administration. In another embodiment, topical administrable compositions are adapted for administration in and/or around the abdomen, back, chest, legs, arms, scalp or other suitable skin surface and may include formulations in which the cannabidiol prodrug is administered in patches, ointments, creams, suspensions, lotions, pastes, gels, sprays, foams or oils.

[0086] In another embodiment, the compositions described herein are suitable for oral administration. In another embodiment, compositions described herein that are orally administrable include formulations in which the cannabidiol prodrug is administered in tablets, capsules, suspensions, syrups or liquids. In an additional embodiment, the composition maybe formulated as extended release or long acting tablet or capsule, hi a further embodiment, the oral

dosage form may be enteric coated using compositions and techniques known to a person of ordinary skill in the art.

[0087] In one embodiment, compositions described herein are suitable for buccal administration. In another embodiment, compositions described herein that are bucally administrable may include formulations in which the cannabidiol prodrug is administered in lozenges, sprays, gels, pastes, dissolvable tablets or dissolvable strips.

[0088] In one embodiment, compositions described herein are suitable for sublingual administration. In another embodiment, compositions described herein that are sublingually administrable may include formulations in which the cannabidiol prodrug is administered in lozenges, sprays, gels, pastes, dissolvable tablets or dissolvable strips.

[0089] In one embodiment, compositions described herein are suitable for injectable administration. In another embodiment, compositions described herein that are injectably administrable may include formulations in which the cannabidiol prodrug is administered as an intravenous, intrathecal, subcutaneous or depot injection.

[0090] In one embodiment, compositions described herein are suitable for rectal administration. In another embodiment, compositions described herein that are rectally administrable may include formulations in which the cannabidiol prodrug is placed in suppositories, ointments, creams, suspensions, solutions, lotions, pastes, gels, sprays, foams or oils.

[0091] In one embodiment, compositions described herein are suitable for vaginal administration. In another embodiment, compositions described herein that are vaginally administrable may include formulations in which the cannabidiol prodrug is placed in suppositories, ointments, creams, suspensions, solutions, lotions, pastes, gels, sprays, foams or oils.

[0092] In one embodiment, compositions described herein are suitable for ocular administration, hi another embodiment, compositions described herein that are ocularly administrable may include formulations in which the cannabidiol prodrug is placed in ointments, suspensions, solutions, gels or sprays.

[0093] In one embodiment, compositions described herein are suitable for nasal administration. In another embodiment, compositions described herein that are nasally administrable may include formulations in which the cannabidiol prodrug is placed in ointments, suspensions, solutions, lotions, pastes, gels, sprays or mists.

EXAMPLES [0094] EXAMPLE 1 [0095] SECTION I. SUMMARY

[0096] The objective was to synthesize cannabidiol and cannabidiol prodrugs and assess the permeation of cannabidiol and its prodrugs through human abdominal skin in vitro. Cannabidiol and five cannabidiol prodrugs were synthesized and tested. Flow through diffusion cells were used for the permeation studies. HEPES -buffered Hanks' balanced salts containing 40% (polyethylene glycol) PEG 400 with gentamicin was used for the receiver compartment. Donor solution was comprised of either 100% propylene glycol (PG) solution or a rubbed in gel formulation. The flux and lag time values of cannabidiol and cannabidiol prodrugs were obtained from the permeation profiles. Drug accumulation in the skin after a 24 or 36 h diffusion experiment was determined as μmol/g wet tissue weight.

[0097] These prodrugs also have improved physiochemical properties that would make them suitable candidates for improved delivery via other routes of administration, including oral, buccal, sublingual, injectable, topical, follicular, nasal, ocular, rectal or vaginal.

[0098] SECTION IL METHODOLOGY

[0099] 1.0 Purpose: Synthesize cannabidiol prodrugs and assess the human skin permeation of cannabidiol and cannabidiol prodrugs in vitro.

Formula: C _3I H ₄₈ N ₂ O ₆ Formula: C ₃₃ H ₅₂ N ₂ O ₆ Formula: C ₃₁ H ₄₆ O ₆ MW: 512 MW: 572 MW: 514 ALL00101 ALL00102 ALL00103

Formula: C ₂₇ H ₄₀ N ₂ O ₄ Formula: C ₂₉ H ₄₄ N ₂ O ₄ Formula: C ₂₁ H ₃₀ N ₂ MW: 456 MW: 484 MW: 314 ALL00104 ALLOO 105 cannabidiol

[00100] 2.0 Skin details

[00101] The skin samples used in the following experiments were obtained from abdominal reduction surgery and dermatomed to a thickness of approximately 200 μm. The skin samples used herein were frozen at -20 ⁰ C for less than six months.

[00102] 3.0 Chemicals

[00103] The chemicals used in the experiment included: acetonitrile (HPLC grade), trifluoroacetic acid, triethylamine, 4-(2-hydroxy ethyl)-piperzine ethane sulfonic acid (HEPES), gentamicin sulfate, p-toluene sulfonic acid, 4-dimethylaminopyridine, isopropyl myristate (IPM), sodium hydroxide, and sodium bicarbonate were purchased through Fisher Scientific (Fairlawn, NJ). Methanol (HPLC grade), acetonitrile (HPLC grade), 3-O-ethoxy-propionic acid, diethyl ether, N^N'-dicyclohexyl carbodiimide, N,N-dimethylgylcine, polyethylene glycol 400 (PEG 400), and sodium sulfate anhydrous were purchased through VWR (West Chester, PA). Propylene glycol (PG), olivetol, chloromethyl chloroformate, ethyl isocyanate, absolute ethanol,

and Hanks' balanced salts modified powder were purchased from Sigma- Aldrich (St. Louis, MO). Petroleum ether, ethyl acetate, hexane, chloroform, and dichloromethane were obtained from the University of Kentucky Chemical Stores (Lexington, KY). (+)-(lS,4R)-p-Mentha-2,8- dien-1-ol was purchased from Norac, Inc.(Azusa, CA). Carbopol ^® 980 was obtained from Noveon, Inc. (Cleveland, OH). Nanopure water was obtained from a Barnstead filtration system (Dubuque, IA).

[00104] 4.0 Synthesis of cannabidiol and cannabidiol prodrugs [00105] 4.1 Synthesis of cannabidiol

[00106] Olivetol (920 mg, 0.00511 mol) and p-toluene sulfonic acid (PTSA) ( 110 mg, 0.000578 mol) were dissolved in benzene; the resulting reaction mixture azeotroped for 2.5 h. The reaction mixture was cooled to room temperature and under argon (+)-(lS,4R)-p-Mentha- 2,8-dien-l-ol (586 mg, 0.00385 mol) was added and stirred at room temperature for 30 min. After completion of the reaction, the reaction mixture was diluted with diethyl ether and washed with saturated sodium bicarbonate solution. The organic layer separated and was dried on anhydrous sodium sulfate. The ether layer evaporated and purified on silica column by using petroleum ether and diethyl ether as eluent (98:2). Finally 300 mg of cannabidiol (300 mg) was collected (24%).

[00107] ¹ HNMR (CDCl ₃ ): 6.23-6.41 (m,2H), 5.99 (s,lH,D ₂ O exchangeable), 5.66 (s,lH), 4.56 (s,lH), 4.49 (bs,lH,D ₂ O exchangeable) 4.46 (s,lH), 3.96 (m,lH), 2.00-2.47 (m,5H),1.87 (m,5H), 1.46-1.83 (m,6H), 1.23-1.48 (m,3H), 0.96-1.00 (m,3H).

[00108] 4.2 Synthesis of ALLOOlOl

[00109] β -alanine,N,N-dimethyl (0.600 mg, 0.00512 mol), N,N'-dicyclohexyl carbodiimide (DCC) (1.05 g, 0.00512 mol), 4-dimethylaminopyridine (DMAP) (85 mg) were dissolved in dichloromethane (DCM) and stirred under nitrogen for 2 h; later cannabidiol (806 mg, 0.00264 mol) in DCM was added. The resulting reaction mixture stirred overnight and the solid was filtered on celite. The crude product was purified on silica column producing 300 mg (26.48%) of final compound (ALLOOlOl).

[00110] 4.3 Synthesis of ALLOO 102

[00111] Cannabidiol (216 mg, 0.0006878 mol) was dissolved in dichloroniethane and reaction mixture cooled to 0 ⁰ C on ice bath and chloromethyl chloroformate (177 mg, 0.001375 mol) was added drop wise up to 30 min and the reaction mixture stirred overnight at room temperature. After completion of the reaction, the reaction mixture was washed with water, the combined organic layer dried, evaporated, and purified on silica column by using petroleum ether and diethyl ether (9:1) as eluent. The title compound was as an oil compound (130 mg, 37%). This oily compound (130 mg, 0.000260 mol) was treated with excess of diethylamine to give the target compound (31.5 mg, 21%).

[00112] ¹ HNMR (CDCl ₃ ): 6.90 (s,2H), 5.83-5.84 (dd, 4H), 5.19 (S,1H, cyclic ethylenic), 4.54-4.55 (d,lH), 4.44-4.44 (d, IH), 3.50-3.55 (m,lH) 2.57-2.62 (m,3H),1.90-2.12 (m,2H),1.34- 1.59, 1.62-1.64 (m,l IH), 1.30-1.33 (m,5H), 0.90-0.92 (m,3H) ¹³ C-NMR (CDCl ₃ ): 13.89, 19.27, 22.30, 23.31, 28.48, 30.02, 30.22, 31.23, 35.05, 37.87, 45.63, 72.19, 72.23, 72.28, 111.05, 122.12, 122.15, 122.18, 134.28, 142.53, 147.13, 149.10, 151.34 ¹ HNMR (CDCl ₃ ): 6.81 (s,2H), 5.42 (s,lH), 4.68 (dd,2H), 3.69 (m,lH), 3.24-3.47 (m,10H), 2.49-2.57 (m,3H), 2.01 (m,2H), 1.69-1.95 (m,12 H), 1.15-1.46 (m,17H), 0.93-0.95 (m,3H) ¹³ C-NMR (CDCl ₃ ): 13.71, 14.35, 14.62, 20.53, 22.76, 23.65, 29.50, 30.82, 30.99, 31.98, 35.62, 38.80, 41.61, 42.20, 46.08, 110.90 125.63, 126.36, 131.61, 141.50, 148.04, 150.30, 153.92.

[00113] 4.4 Synthesis of ALL00103

[00114] 3-O-ethoxy-propionic acid (0.507 mg, 0.0042 mol), DCC (889 mg, 0.00429 mol), and DMAP (138 mg) were dissolved in DCM under nitrogen, stirring for 2 h. Cannabidiol (450 mg, 0.00143 mol) was added in DCM for 30 min and resulting reaction mixture stirred overnight. The solid was filtered through celite, filtrate purified by using (75:25) petroleum ether, and diethyl ether was used as eluent giving 210 mg (51.4%) an oily final compound.

[00115] ¹ HNMR (CDCl ₃ ): 6.78 (s,2H), 5.20 (s,lH), 4.45-4.53 (dd,2H), 3.77-3.80 (m,4H), 3.58 (m,lH), 3.50-3.57 (t,4H), 2.57-2.90 (m,4H), 2.51-2.56 (t,2H), 2.10 (m,lH), 2.00 (m,lH), 1.61-1.90 (m,2H), 1.57-1.65 (m,9H), 1.30-1.32 (m,4H), 1.21-1.29 (t,6H), 0.85-0.90 (m,3H) ¹³ C- NMR (CDCl ₃ ): 14.25, 15.35,19.84, 22.68, 23.59, 28.97, 30.56, 30.70, 31.67, 35.39, 38.46, 45.82, 65.78, 66.68, 111.13, 124.69, 126.02, 141.94, 147.80, 149.58, 169.78.

[00116] 4.5 Synthesis of ALLOO 104

[00117] Cannabidiol (284 mg, 0.000904 mol) and triethylamine (0.482 mL, 0.00226 mol) were dissolved in DCM and stirred at room temperature for 1 h. Ethyl isocyanate (0.1419 mL, 0.00180 mol) was added drop wise for 10 min to give the reaction mixture which stirred overnight. The solvents were evaporated and the crude product purified on silica column by using (8:2) hexane:ethyl acetate as eluent. The final compound (260 mg, 63%) collected was a semi solid.

[00118] ¹ HNMR (CDCl ₃ ): 6.77 (s,2H), 5.30 (m,lH), 4.11 (bs,2H), 4.42 (s,lH), 4.85 (s,lH), 3.15 (m,4H), 2.46-2.64 (m,3H), 2.10 (m,2H), 1.88 (2H), 1.25-1.66 (m,10H), 1.30 (m,5H), 1.17- 1.22 (m,5H), 0.88 (m,3H) ¹³ C-NMR (CDCl ₃ ): 14.36, 14.53, 15.53, 19.87, 22.80, 23.71, 25.02, 29.17, 30.75, 30.90, 31.87, 35.61, 36.44, 36.97, 38.26, 46.24, 111.14, 124.60, 127.12, 132.37, 141.76, 147.97, 149.87, 154.26.

[00119] 4.6 Synthesis of ALL00105

[00120] Cannabidiol (280 mg, 0.000890 mol) was added to activate DCC (550 mg, 0.00267 mol), DMAP (429 mg, 0.00133 mol), and N,N-dimethylglycine (275 mg, 0.002678 mol) in DCM at room temperature. After overnight stirring under nitrogen, the solid in the reaction mixture was filtered though celite and the reaction mixture purified on silica column by using chloroform and methanol (97:3) as eluent gave 70 mg of final compound (10.07%).

[00121] ¹ HNMR (CDCl ₃ ): 6.42 (s,2H), 5.12 (s,lH), 4.20-4.40 (dd,2H), 2.21 (s,4H), 2.34- 2.40 (m,lH), 2.20-2.34 (m,13H), 2.00-2.10 (m,3H), 1.29-1.42 (m,10H), 1.13-1.26 (m,4H), 0.91- 0.92 (m,3H) ¹³ C-NMR (CDCl ₃ ): 14.36, 20.16, 22.80, 23.90, 29.12, 30.67, 30.85, 31.75, 35.53, 38.86, 45.57, 45.86, 60.16, 111.23, 124.69, 125.94, 132.71, 142.17, 147.86, 149.38, 168.73.

[00122] 5.0 In vitro skin permeation studies

[00123] 5.1 Preparation of receiver fluid

[00124] 1 L of receiver fluid was prepared by measuring 1 L of nanopure water into a graduated cylinder. 90% of the water was added to an Erlenmeyer flask. Hanks' salts (1 bottle) were added to the water along with 5.96 g of HEPES and 0.35 g of sodium bicarbonate. The pH of the solution was adjusted with 1 N sodium hydroxide solution to pH 7.4. The remaining water was added and the receiver fluid was filtered through a 0.2 μ filter (Millipore, Billerica, MA). 50

mg of gentamicin was added to the filtered receiver fluid and 400 mL of the receiver fluid was removed and replaced with 400 mL of PEG 400.

[00125] 5.2 Preparation of drug formulations

[00126] Two different formulations were used for charging the donor compartment. Drugs were made up in either 100% PG or a gel formulation. For the PG solution, approximately 50- 120 mg of the appropriate drug was weighed into a glass silanized culture tube. The gel formulation resulted from the mixing of absolute ethanol, nanopure water, IPM, Carbopol ^® 980, 0.1 N aqueous sodium hydroxide solution and the respective drug.

[00127] 5.3 Permeation experiments

[00128] Dermatomed skin harvested from abdominoplasty, stored at -20 ⁰ C, was used for the experiments. A PermeGear flow-through (In-Line, Riegelsville, PA) diffusion cell system was used for the skin permeation studies.

[00129] Diffusion cells were kept at 32°C with a circulating water bath. Human epidermal skin was arranged in the diffusion cell with stratum corneum (upper layer of skin) facing the donor compartment. Permeation area of the skin was 0.95 cm ² . Data was collected from a human skin donor with three to four diffusion cells per treatment.

[00130] Receiver solution was HEPES -buffered Hanks' balanced salts with gentamicin containing 40% PEG 400 at a pH of 7.4 and flow rate was adjusted to 0.8 mlVh. Each cell was charged with 0.050 mL of the respective drug formulation (donor solution) or with 0.035 mL of gel formulation which was rubbed into the skin for 15 sec with a Teflon coated rod. The formulation was applied to ensure complete coverage. Diffusion cells were covered with a cap for the duration of the study.

[00131] Samples were collected into scintillation vials in 3 h increments for either 24 h or 36 h. All the samples were stored at 4°C until extracted. An aliquot (0.5 mL) of the diffusion sample was placed into a silanized HPLC vial and 0.5 mL of acetonitrile was added to the sample, capped and vortexed.

[00132] At the end of the experiment, the skin tissue was removed from the diffusion cell, rinsed with nanopure water, and blotted dry with a paper towel. The skin was tape stripped twice using book tape (Scotch™, 3M, St. Paul, MN) to remove drug formulation adhering to the tissue

surface. The area of skin in contact with the drug was cut out, chopped up and placed in a pre- weighed scintillation vial. Ten rnL of acetonitrile was added to the vial and drug was extracted from the skin by shaking at room temperature overnight. The following day a 0.1 niL aliquot was removed and diluted with an additional 0.9 mL of acetonitrile. The diluted sample was added to the silanized HPLC vial for analysis.

[00133] At the end of the experiment, a 0.01 mL aliquot of the PG donor solution was removed and added to a scintillation vial containing 10 mL of acetonitrile. The vials were vortexed and then sonicated for 15 min. An aliquot of 1 mL was removed and transferred into a silanized HPLC vial for analysis.

[00134] 6.0 Analytical method

[00135]

[00136] 7.0 Data analysis

[00137] Cumulative quantity of drug collected in the receiver compartment was plotted as a function of time. The flux value for a given experiment was obtained from the slope of a steady

state portion of the cumulative amount of drug permeated vs. time plot. Lag time was obtained from the x-intercept of the steady state portion of the cumulative amount of drug permeated vs. time plot. In Tables 11-14, the combined results of the delivered prodrug and cannabidiol from the prodrug are listed as "total cannabidiol." These values represent the data as total cannabidiol equivalents delivered in the form of cannabidiol and/or prodrug.

[00138] SECTION III. RESULTS

[00139] No melting points were reported for cannabidiol or the cannabidiol prodrugs due to all compounds being in oil form. ALLOOlOl and ALLOO 102 permeated through the human skin as cannabidiol. No intact prodrug was found in the diffusion samples (receiver samples) for either ALLOOlOl or ALL00102. Both cannabidiol and intact prodrug were found in the skin disposition (tissue) samples. ALLOOlOl and ALLOO 102 did have higher total cannabidiol in the skin compared to cannabidiol. No flux enhancement was seen with ALLOOlOl or ALL00102 prodrugs. ALLOO 103 and ALLOO 104 did not permeate (or were below LOD) through the skin with both the PG donor solution and gel formulation. Some intact prodrug was found in the skin samples but very little cannabidiol was detected for ALLOO 103 and ALLOO 104. The mean flux enhancement between the two ALLOO 1005 gel formulation experiments was 2.5. ALLOO 105 permeated primarily as intact prodrug. The cumulative amount of total cannabidiol equivalents (nmol) delivered from ALL00105 was 3.1-5.9 fold higher when compared to cannabidiol. The same results were not seen with the propylene glycol donor solution which did not show any enhancement. Besides the enhanced flux with ALL00105, the lag time (gel formulation studies) of total cannabidiol was decreased 7-8 h compared to the parent drug. Decreased lag time benefits patients by delivering the drug more quickly which is beneficial for pain management and nausea. The other cannabidiol prodrugs may be successful if drug solutions were formulated with enhancers or may be useful in targeting topical treatment administration instead of transdermal administration (for systemic blood levels of drug).

[00140] Table 1. Cannabidiol and cannabidiol prodrugs

[00141] Table 2. Permeation data of cannabidiol (n=3) in propylene glycol

24 h skin cone, FlUX Lag time

24 h cumulative

Compound amt (nmol) (μmol/g) (nmol/cm ² /h) (h) cannabidiol (CBD) 12.2 ± 1.8

190.8 ± 10.7 222.7 ± 74.0 10.4 ± 2.0

Wherein "n" is the number of skin samples tested.

[00142] Table 3. Permeation data of CBD-low (n=2), ALLOOlOl (n=2), ALL00102 (n=2), and CBD-high (n=3) in propylene glycol

Wherein "n" is the number of skin samples tested.

[00143] Table 4. Permeation data of CBD-low (n=2), ALL00102 (n=3), and CBD-high (n=2) in propylene glycol

Wherein "n" is the number of skin samples tested.

[00144] Table 5. Permeation data of CBD-low (n=4), ALL00102 (n=2), and CBD-high (n=2) in propylene glycol

Wherein "n" is the number of skin samples tested.

[00145] Table 6. Permeation data of CBD (n=4) in propylene glycol

24 h skin cone, FlUX Lag time

24 h cumulative

Compound amt (nmol) (μmol/g) (nmol/cm ² /h) (h)

CBD 49.2 ± 15.2 15.1 ± 3.4

27.5 ± 32.5 4.3 ± 2.4

Wherein "n" is the number of skin samples tested.

[00146] Table 7. Permeation data of CBD (n=3) in propylene glycol

36 h skin cone, FlUX Lag time

36 h cumulative

Compound amt (nmol) (μmol/g) (nmol/cm ² /h) (h)

CBD 13.4 ± 9.3

44.1 ± 15.5 15.7 ± 5.4 1.0 ± 0.7

Wherein "n" is the number of skin samples tested.

[00147] Table 8. Permeation data of CBD (n=3) in gel formulation

36 h skin cone, Flux Lag time

36 h cumulative

Compound amt (nmol) (μmol/g) (nmol/cm ² /h) (h)

CBD 1.4 ± 1.7

28.4 ± 8.8 52.0 ± 42.7 2.0 ± 2.3

Wherein "n" is the number of skin samples tested.

[00148] Table 9. Permeation data of CBD (n=3) and ALL00102 (n=3) in gel formulation

Wherein "n" is the number of skin samples tested.

[00149] Table 10. Permeation data of CBD (n=4) and ALL00102 (n=4) in propylene glycol

Wherein "n" is the number of skin samples tested.

[00150] Table 11. Permeation data of CBD (n=3) and ALL00105 (n=3) in gel formulation

* total CBD= total cannabidiol equivalents delivered in the form of cannabidiol and/or prodrug

Wherein "n" is the number of skin samples tested.

[00151] Table 12. Permeation data of CBD (n=2) and ALL00105 (n=2) in gel formulation

Wherein "n" is the number of skin samples tested.

[00152] Table 13. Permeation data of CBD (n=l) and ALL00105 (n=2) in propylene glycol

Wherein "n" is the number of skin samples tested.

[00153] Table 14. Permeation data of CBD (n=3) and ALL00103 (n=4) in propylene glycol

Wherein "n" is the number of skin samples tested. [00154] EXAMPLES 2, 2A AND 2B

[00155] Except as where indicated below, the methodology used in Examples 2A and 2B was the same as the methodology used in Example 2.

[00156] SECTION I. SUMMARY

[00157] The objective was to synthesize cannabidiol prodrugs and assess the permeation of cannabidiol and various prodrugs of cannabidiol through human abdominal skin in vitro. Cannabidiol and numerous cannabidiol prodrugs were synthesized and several were tested. Flow through diffusion cells were used for the permeation studies. HEPES -buffered Hanks' balanced

salts containing 40% PEG 400 with gentamicin or 40% aqueous PEG 400 with gentamicin were used for the receiver compartment. Donor solution was comprised of 90:8:2 PG:H ₂ θ:IPM solution or gel formulation. The flux and lag time values of cannabidiol and cannabidiol prodrugs were obtained from the permeation profiles. Drug accumulation in the skin after a 24, 30 or 42 h diffusion experiment was determined as μmol/g wet tissue weight.

[00158] These prodrugs also have improved physicochemical properties that would make them suitable candidates for improved delivery via other routes of administration, including oral, buccal, sublingual, injectable, topical, follicular, nasal, ocular, rectal or vaginal.

[00159] SECTION II. METHODOLOGY

[00160] 1.0 Purpose: The purpose of the example was to synthesize cannabidiol prodrugs and assess the human skin permeation of cannabidiol and cannabidiol prodrugs in vitro.

Formula: C _3O H ₄₆ O ₇ Formula: C^H ₆₂ Oi ₂ MW: 518.68 MW: 722.90 ALL00131 ALLOOl 32

Formula: C ₂₆ H ₄ QN ₂ O ₃ Formula: C ₃₁ H _5O N ₄ O ₄ Formula: C ₂₄ H ₃₄ O ₅ MW: 428.61 MW: 542.75 MW: 402.52 ALLOO135 ALLOO 136 ALLOO 137

Formula: C ₂₇ H ₃₈ Og Formula: C ₂₈ H ₄₂ O ₆ MW: 490.59 MW: 474.63 ALLOO 139 ALLOO 140

Formula: C ₃₅ H ₅₄ OiO Formula: C ₂₅ H ₃₄ O ₄ MW: 634.80 MW: 398.54 ALL00141 ALL00142

Formula: C ₂₅ H ₃₄ O ₆ Formula: C ₂₃ H ₃ o0 ₄ Formula: C ₂₂ H ₃ o0 ₃ MW: 430.53 MW: 370.48 MW: 342.47 ALL00143 ALL00145 ALLOO 146

Formula: C ₂₅ H ₃₄ O ₆ Formula: C ₂₅ H ₃₇ NO ₃ Formula: C ₂₃ H ₃₂ O ₄ MW: 430.53 MW: 399.57 MW: 372.50

ALL00147 ALL00148 ALL00149

Formula: C ₂₆ H ₃₉ NO ₃ Formula: C ₃ ]H _4S N ₂ O ₆ Formula: C ₂₆ H ₄₄ N ₂ O ₄ MW: 413.59 MW: 512.72 MW: 484.67 ALLOO 150 ALLOOlOl ALLOO 105

Formula: C ₂₄ H ₃₄ O ₄ MW: 386.52 ALL00151

[00161] 2.0 Skin details

[00162] The skin samples used in the following experiments were obtained from abdominal reduction surgery and dermatomed to a thickness of approximately 200 μm. The skin samples used herein were frozen at -20 ⁰ C for less than six months.

[00163] 3.0 Chemicals

[00164] Chemicals used in the experiment included: trifluoroacetic acid, triethylamine (TEA), 4-(2-hydroxy ethyl)-piperzine ethane sulfonic acid (HEPES), gentamicin sulfate, isopropyl myristate (IPM), sodium hydroxide, octanethiol, 4-dimethylaminopyridine (DMAP), methyl chloroformate, and sodium bicarbonate (NaHCO ₃ ) were purchased through Fisher Scientific (Fairlawn, NJ). Methanol (HPLC grade), acetonitrile (HPLC grade), N ₅ N ¹ - dicyclohexyl carbodiimide (DCC), N,N-dimethylgylcine, polyethylene glycol 400 (PEG 400), formic acid, acetyl chloride, 3-dimethylaminopropionic acid hydrochloride, 1,8- diazabicyclo[5.4.0]undec-7-ene (DBU), and sodium sulfate (NaSO ₄ ) anhydrous were purchased

through VWR (West Chester, PA). Propylene glycol (PG), olivetol, absolute ethanol, triethylamine trihydrofluoride, 2-[2-92-methoxyethoxy)ethoxy] acetic acid, methyl (R)-(+)-2,2- dimethyl-l,3-dioxolane-4-carboxylate, tetrahydrofuran (THF), triphosgene, L-(t- butyldimethylsilyloxy)lactic acid and Hanks' balanced salts modified powder were purchased from Sigma- Aldrich (St. Louis, MO). mono-Fmoc-l,4-butanediamine hydrochloride was purchased from Novabiochem (San Diego, CA). Ethyl acetate, hexane, and dichloromethane (DCM) were obtained from the University of Kentucky Chemical Stores (Lexington, KY). (+)- (lS,4R)-p-Mentha-2,8-dien-l-ol and olivetol were purchased from Norac, Inc.(Azusa, CA). Carbopol ^® 980 was obtained from Noveon, Inc. (Cleveland, OH). Cannabidiol was purchased from Cayman Chemical Company (Ann Arbor, MI). Nanopure water was obtained from a Barnstead NANOpure ^® Diamond™ Ultrapure water filtration system (Dubuque, IA). Argon and pre-purified nitrogen were purchased from Scott Gross Company (Lexington, KY). The compound 3,6,9,12-tetraoxatridecanoic acid was synthesized according to the procedures found in Macromolecules, 39 (12), 3978 -3979, 2006.

[00165] 4.0 Synthesis of cannabidiol prodrugs

[00166] 4.1 Synthesis of ALLOOlOl (CBD bis(3-(dimethylamino)propionate)) and ALL00150 (CBD 3-(dimethylamino)propionate)

[00167] Cannabidiol (100 mg, 0.00032 mol), 3-dimethylaminopropionic acid hydrochloride (123 mg, 0.00080 mol), and DMAP (117 mg, 0.00096 mol) were combined in 1 mL dry dichloromethane. The solution was stirred for 5 min at ambient temperature. DCC (198 mg, 0.00096 mol) was added to the mixture. The mixture was allowed to stir for 5 h at ambient temperature. Hexane was added and the precipitate removed by filtration. The solution was reduced to a small volume under nitrogen. ALLOOlOl and ALLOO 150 were separated and isolated using a semi-preparatory Cg column with ACN:water (70:30). ACN was removed from the eluent fraction for each fraction by rotary evaporation. The remaining aqueous layer was partitioned with DCM and the DCM dried over sodium sulfate. DCM was removed under a nitrogen stream and vacuum. The purified products appeared as transparent, viscous oil with light amber color.

[00168] ALLOOlOl was analyzed by LC/MS (Waters; Milford, MA) in electrospray positive mode. Masses were observed at 513.42 (M+l, 70%), 257.34 (100%) and 123.06 (15%).

[00169] For ALL00150, the ¹ H NMR (400 MHz, CDCl ₃ ) was as follows: δ= 6.55(1H, br s, ArH); 6.4O(1H, d, J=1.8, ArH); 5.98(1H, br s, OH); 5.53(1H, br s, H-2); 4.59-4.62(1H, m); 4.45(1H, br s); 3.45-3.59(1H, m, H-3); 2.60-2.83(4H, m); 2.41-2.55(3H, m); 2.30(6H, s, NMe ₂ ); 2.16-2.27(1H, m); 2.03-2.12(1H, m); 1.70-1.84(2H, m); 1.77(3H, br s, 7-Me); 1.52-1.62(2H, m); 1.61(3H, s, 10-Me); 1.24-1.38(4H, m); 0.88(3H, t, J=7.0, CH ₂ CH ₃ )

[00170] 4.2 Synthesis of ALL00105 (CBD bis(N,N-dimethylglycinate)) and ALL00148 (CBD N,N-dimethylglycinate)

[00171] Cannabidiol (200 mg, 0.00064 mol), N,N-dimethylglycine (196.8 mg, 0.00191 mol), and DMAP (38.9 mg, 0.00032 mol) were combined in 1 mL dry dichloromethane. The solution was stirred for 5 min at ambient temperature. DCC (459.3 mg, 0.00223 mol) was added to mixture. The mixture was allowed to stir overnight at ambient temperature. Hexane was added and precipitate removed by filtration. The solution was reduced to a small volume under nitrogen. ALLOO 105 and ALLOO 148 were separated and isolated using a semi-preparatory Cg column with ACNrwater (75:25). ACN was removed from the eluent fraction for each fraction by rotary evaporation. The remaining aqueous layer was partitioned with DCM and DCM dried over sodium sulfate. DCM was removed under a nitrogen stream and vacuum. The purified products appeared as transparent, viscous oil with light amber color.

[00172] ALLOO 105 was analyzed by LC/MS in electrospray positive mode. Masses were observed at 485.31 (M+l).

[00173] ALLOO 148 was analyzed by LC/MS in electrospray positive mode. Masses were observed at 417.15 (M+18, 30%), 315.23 (CBD+1, 100%).

[00174] 4.3 Synthesis of ALL00131 (CBD 3,6,9, 12-tetraoxatridecanoate) and ALL00132 (CBD di(3, 6,9, 12-tetraoxatridecanoate))

[00175] Cannabidiol (100 mg, 0.00033 mol), 3,6,9,12-Tetraoxatridecanoic acid (98.9 mg, 0.00045 mol) prepared according to Macromolecules, 39 (12), 3978 -3979, 2006, and DMAP (11.7 mg, 0.00010 mol) were combined in 1 mL dry dichloromethane. The solution was stirred for 5 min at ambient temperature. DCC (111.4 mg, 0.00046 mol) was added to the mixture. The mixture was allowed to stir for 2 h at ambient temperature. Hexane was added and precipitate removed by filtration. The solution was reduced to a small volume under nitrogen. ALLOOl 31

and ALLOO 132 were separated and isolated using a semi-preparatory silica column with hexane:ethyl acetate gradient. Solvent from each collected fraction was removed under vacuum. The purified products appeared as transparent, viscous oil with light amber color.

[00176] For ALL00131, the ¹ H NMR (400 MHz, CDCl ₃ ) was as follows: δ= 6.56QH, br s, ArH); 6.43(1H, d, J=1.8, ArH); 6.01(1H, br s, OH); 5.53(1H, br s, H-2); 4.58-4.62(1H, m); 4.43QH, br s); 4.35(2H, br s, OCH ₂ CO ₂ ); 3.76-3.86(2H, m, PEG); 3.63-3.76(8H, m, PEG); 3.54-3.57(2H, m, PEG); 3.47QH, br s, H-3); 3.38(s, 3H, CH ₂ OCH ₃ ); 2.47-2.53(2H, m, benzylic CH ₂ ); 2.4O-2.47(1H, m); 2.14-2.25(1H, m); 2.02-2.14(1H, m); 1.65-1.86(2H, m); 1.77(3H, br s, 7-Me); 1.52-1.64(2H, m); 1.58(3H, s, 10-Me); 1.23-1.37(4H, m); 0.88(3H, t, J=7.0, CH ₂ CH ₃ ).

[00177] For ALL00132, the ¹ H NMR (400 MHz, CDCl ₃ ) was as follows: δ= 6.75(2H, s, ArH); 5.18(1H, br s, H-2); 4.52-4.55(1H, m); 4.42-4.45(1H, m); 4.37,4.34 and 4.22(4H, br singlets, OCH ₂ CO ₂ ); 3.76-3.86(4H, m, PEG); 3.63-3.76(16H, m, PEG); 3.53-3.57(4H, m, PEG); 3.43-3.51(1H, m, H-3); 3.38(s, 6H, CH ₂ OCH ₃ ); 2.51-2.62(3H, m); 2.07-2.20(1H, m); 1.97- 2.06(1H, m); 1.67-1.82(2H, m); 1.66(3H, br s, 7-Me); 1.52-1.63(2H, m); 1.54(3H, s, 10-Me); 1.23-1.38(4H, m); 0.88(3H, t, J=7.0, CH ₂ CH ₃ )

[00178] 4.4 Synthesis of ALL00135 (CBD 4-aminobutyl carbamate) and ALL00136 (CBD bis(4-aminobutyl carbamate))

[00179] To a stirred solution of mono-Fmoc-l,4-butanediamine hydrochloride (461 mg, 1.33 mmol) in saturated NaHCO ₃ aqueous solution (33.3 mL) and dichloromethane (22.2 mL) was added to triphosgene (592 mg, 2.0 mmol) in dichloromethane (5 mL) at ambient temperature. After stirring for 1 h, the product was extracted with dichloromethane (40 mL), and dichloromethane layer was dried over anhydrous Na ₂ SO ₄ and concentrated. The residue was dissolved in ethyl acetate and product was precipitated with addition of hexane. Fmoc-4- aminobutyl isocyanate was collected by filtration as a white solid (305 mg, 68%).

[00180] Cannabidiol (44 mg, 0.00014 mol), Fmoc-4-aminobutyl isocyanate (68.0 mg, 0.00020 mol), and TEA (20 mg, 0.00038 mol) were combined in 2 mL dry dichloromethane under argon. The solution was allowed to stir overnight. The reaction mix was filtered. The protected compounds were separated and isolated using a semi-preparatory silica column with hexane: ethyl acetate gradient. The solvent from each collected fraction was taken to dryness under a nitrogen stream and reconstituted in THF.

[00181] Solutions of DBU (diluted 100-fold in THF) and octanethiol (diluted 10-fold in THF) were prepared. DBU solution (0.2 mL) and ocatanethiol solution (0.1 mL) were added to each solution containing a protected compound and stirred for 0.5-1 h. ALLOO 135 and ALL00136 were isolated using silica column chromatography and eluted with DCM:methanol (80:20). The purified products appeared as transparent, viscous oil with light amber color.

[00182] ALLOO 135 was analyzed by LC/MS in electrospray positive mode. Masses were observed at 429.26 (M+l, 100%), 315.16 (CBD+1, 65%), and 153.13 (22%).

[00183] ALLOO136 was analyzed by LC/MS in electrospray positive mode. Masses were observed at 543.32 (M+l, 30%), 315.16 (CBD+1, 70%), 292.76 (100%) and 272.24 (45%).

[00184] 4.5 Synthesis of ALL00137 (CBD (S)-2,3-dihydroxypropanoate) and ALL00139 (CBD bis((S)-2,3-dihydroxypropanoate))

[00185] Cannabidiol (100 mg, 0.00032 mol), (S)-2,2-dimethyl-l,3-dioxalane-4-carboxylate (151.4 mg, 0.00035 mol), and DMAP (11.7 mg, 0.00010 mol) were combined in 1 mL dry dichloromethane. The solution was stirred for 5 min at ambient temperature. DCC (92.3 mg, 0.00045 mol) was added to the mixture. The mixture was allowed to stir overnight at ambient temperature. Hexane was added and precipitate removed by filtration. The solution was reduced to a small volume under nitrogen. Acetonides of ALLOO 137 and ALLOO 139 were separated and isolated using a semi-preparatory silica column with hexane: ethyl acetate gradient. The solvent from each collected fraction was removed under vacuum. The purified products appeared as transparent, viscous oil with light amber color. Acetonides of ALLOO 137 and ALLOO 139 were deprotected using 1-octanethiol/zinc triflate and purified using silica column chromatography (HPLC) with hexane:ethyl acetate (7:3).

[00186] ALLOO 137 was analyzed by LC/MS in electrospray positive mode. Masses were observed at 420.25 (M+l 8, 100%), 403.18 (M+l, 65%), 315.23 (CBD+1, 15%).

[00187] ALLOO 139 was analyzed by LC/MS in electrospray positive mode. Masses were observed at 508.23 (M+ 18, 100%), 491.15 (M+l, 18%), 315.16 (CBD+1, 5%).

[00188] 4.6 Synthesis of ALL00140 (CBD 2-[2-(2-methoxyethoxy)ethoxy]acetate) and ALL00141 (CBD bis(2-[2-(2-methoxyethoxy)ethoxy]acetate))

[00189] Cannabidiol (105 mg, O.OOO33 mol), 2-[2-(2-methoxyethoxy)ethoxy] acetic acid (83.3 mg, 0.00047 mol), and DMAP (12.3 mg, 0.00011 mol) were combined in 1 niL dry dichloromethane. The solution was stirred for 5 min at ambient temperature. DCC (117 mg, 0.00056 mol) was added to the mixture. The mixture was allowed to stir for 5 h at ambient temperature. Hexane was added and precipitate removed by filtration. The solution was reduced to a small volume under nitrogen. ALL00140 and ALL00141 were separated and isolated using a semi-preparatory silica column with hexane: ethyl acetate gradient. The solvent from each collected fraction was removed under vacuum. The purified products appeared as transparent, viscous oil with light amber color.

[00190] ALLOO 140 was analyzed by LC/MS in electrospray positive mode. Masses were observed at 492.37 (M+18, 100%) and 475.37 (M+l, 35%).

[00191] ALL00141 was analyzed by LC/MS in electrospray positive mode. Masses were observed at 652.51 (M+18, 100%) and 635.52 (M+l, 10%).

[00192] 4.7 Synthesis of ALLOO 142 (CBD diacetate)

[00193] Cannabidiol (42 mg, 0.00013 mol), acetyl chloride (26.3 mg, 0.00033 mol), and TEA (35 mg, 0.00039 mol) were combined in 0.5 mL dry dichloromethane. The solution was allowed to stir overnight. Hexane was added and reaction mix filtered. ALLOO 142 was isolated using silica column chromatography and hexane:ethyl acetate (9:1). The purified product appeared as transparent, viscous oil with light amber color.

[00194] ALL00142 was analyzed by LC/MS in electrospray positive mode. Masses were observed at 416.22 (M+18, 100%), 399.14 (M+l, 10%).

[00195] 4.8 Synthesis of ALL00143 (CBD bis(methyl carbonate))

[00196] Cannabidiol (44 mg, 0.00013 mol), methyl chloroformate (31.7 mg, 0.00034 mol), and TEA (18.4 mg, O.OOO35 mol) were combined in 2 mL dry dichloromethane. The solution was allowed to stir overnight. Hexane was added and the reaction mix filtered. ALLOO 143 was isolated using silica column chromatography and hexane:ethyl acetate (9:1). The purified product appeared as transparent, viscous oil with light amber color.

[00197] For ALL00143, the ¹ H NMR (400 MHz, CDCl ₃ ) was as follows: δ= 6.82(2H, s, ArH); 5.17-5.21(1H, m, H-2); 4.51-4.55(1H, m); 4.42-4.46(1H, m); 3.86(6H, s, OCO ₂ CH ₃ );

3.60-3.68(1H, m, H-3); 2.65-2.73(1H, m); 2.53-2.59(2H, m, benzylic CH ₂ ); 2.16-2.28(1H, m); 1.93-2.O1(1H, m); 1.66-1.81(2H, m); 1.65(3H, br s, 7-Me); 1.54-1.63(2H, m); 1.58-1.60(3H, m, 10-Me); 1.24-1.37(4H, m); 0.88(3H, t, J=7.0, CH ₂ CH ₃ ).

[00198] 4.9 Synthesis of ALL00145 (CBD diformate) and ALLOO 146 (CBD formate)

[00199] Cannabidiol (150 mg, 0.00043 mol), formic acid (59.25 mg, 0.00129 mol), and DMAP (36.6 mg, 0.00030 mol) were combined in 1 mL dry dichloromethane. The solution was stirred for 5 min at ambient temperature. DCC (150 mg, 0.00142 mol) was added to the mixture. The mixture was allowed to stir for 4 h at ambient temperature. Hexane was added and precipitate removed by filtration. The solution was reduced to a small volume under nitrogen. ALLOO 145 and ALLOO 146 were separated and isolated using a semi-preparatory silica column with hexane:ethyl acetate (97:3). The solvent from each collected fraction was removed under vacuum. The purified products appeared as transparent, viscous oil with light amber color.

[00200] For ALL00145, the ¹ H NMR (400 MHz, CDCl ₃ ) was as follows: δ= 8.16(2H, br s, CHO); 6.78(2H, s, ArH); 5.09-5.13(1H, m, H-2); 4.52-4.56 (IH, m); 4.41-4.45(1H, m); 3.60- 3.69(1H, m, H-3); 2.64-2.72(1H, m); 2.54-2.62(2H, m, benzylic CH ₂ ); 2.08-2.20(1H, m); 1.96- 2.05(1H, m); 1.75-1.82(1H, m); 1.66-1.75(1H, m); 1.654(3H, br s, 7-Me); 1.65-1.54(2H, m); 1.58-1.60(3H, m, 10-Me); 1.24-1.39(4H, m); 0.89(3H, t, J=7.0, CH ₂ CH ₃ ).

[00201] For ALL00146, the ¹ H NMR (400 MHz, CDCl ₃ ) was as follows: δ= 8.18(1H, s, CHO); 6.78(1H, br s, ArH); 6.37(1H, d, J=1.5, ArH); 6.1O(1H, br s, OH); 5.53(1H, br s, H-2); 4.55-4.58(1H, m); 4.36(1H, br s); 3.62-3.72(1H, m, H-3); 2.48-2.54(2H, m, benzylic CH ₂ ); 2.41- 2.46(1H, m); 2.17-2.30(1H, m); 2.05-2.15(1H, m); 1.68-1.86(2H, m); 1.79(3H, br s, 7-Me); 1.53-1.63(2H, m); 1.60(3H, br s, 10-Me); 1.24-1.38(4H, m); 0.88(3H, t, J=7.0, CH ₂ CH ₃ ).

[00202] 4.10 Synthesis ALL00147 (CBD diglycolate)

[00203] To a stirred solution of cannabidiol (31.4 mg, 0.1 mmol) and (t- butyldimethylsilyloxy)acetic acid (57.5 mg, 0.26 mmol) in dichloromethane (0.4 mL), 4- dimethylaminopyridine (10.3 mg, 0.05 mmol) was added followed by N,N'- dicyclohexylcarbodiimide (61.9 mg, 0.3 mmol). The mixture was stirred at ambient temperature for 4 h. The mixture was diluted with hexane (0.4 mL), filtered, concentrated under a reduced

pressure, and chromatographed on silica gel with hexane-ethyl acetate (gradient 40:1, 20:1, 10:1) to afford CBD bis((t-butyldimethylsilyloxy)acetate) (44.4 mg, 67.4%) as an oil.

[00204] Cannabidiol bis((t-butyldimethylsilyloxy)acetate) was dissolved in dichloromethane (0.2 mL), cooled to -15°C and treated with 0.2 mL of cold 2 M solution of triethylamine trihydrofluoride. The reaction mixture was left at 5°C for 65 h. The mixture was poured into an excess of aqueous saturated sodium bicarbonate/ethyl acetate cooled to 0 ⁰ C with vigorous stirring. The aqueous layer was extracted twice with ethyl acetate, combined organic extracts were dried over anhydrous sodium sulfate and concentrated. Residue was chromatographed on silica gel with hexane-ethyl acetate (gradient 3:1, 2:1, 1:1) to afford 18.9 mg (65%) of cannabidiol diglycolate (ALLOO 147) as an oil.

[00205] For ALL00147, the ¹ H NMR (400 MHz, CDCl ₃ ) was as follows: δ= 6.79(2H, s, ArH); 5.17(1H, br s, H-2); 4.52-4.57(1H, m); 4.41-4.47(1H, m); 4.39, 4.36 and 4.22(4H, br singlets, OCH ₂ CO ₂ ); 3.42-3.50(1H, m, H-3); 2.48-2.62(3H, m); 2.36(2H, t, J=5.6, OH); 2.08- 2.2O(1H, m); 1.99-2.08(1H, m); 1.68-1.83(2H, m); 1.66(3H, br s, 7-Me); 1.57-1.65(2H, m); 1.52-1.54(3H, s, 10-Me); 1.26-1.38(4H, m); 0.89 (3H, t, J=7.0, CH ₂ CH ₃ ).

[00206] 4.11 Synthesis ALL00149 (CBD glycolate)

[00207] To a stirred solution of cannabidiol (125.8 mg, 0.4 mmol) and (t- butyldimethylsilyloxy)acetic acid (112.9 mg, 0.51 mmol) in dry dichloromethane (1 mL) was added of 4-dimethylaminopyridine (6.2 mg, 0.03 mmol) followed by N,N'- dicyclohexylcarbodiimide (123.8 mg, 0.6 mmol). The mixture was stirred at ambient temperature for 1 h. Additional amounts of the acid (26.6 mg) and N,N'- dicyclohexylcarbodiimide (31 mg) were added and stirring was continued for 1 h. Mixture was diluted with hexane (2 mL), filtered, and concentrated. The crude product was purified by preparative reverse phase HPLC (C8 column) with acetonitrile-water to afford cannabidiol (t- butyldimethylsilyloxy)acetate as an oil.

[00208] Cannabidiol (t-butyldimethylsilyloxy)acetate (80 mg) was dissolved in dry dichloromethane (0.25 mL), cooled to -15°C and treated with 0.25 mL of cold 2 M solution of triethylamine trihydrofluoride in dichloromethane. The reaction mixture was left at 5°C for 65 h. The mixture was poured to an excess of aqueous saturated sodium bicarbonate/ethyl acetate cooled to O ⁰ C with vigorous stirring. Aqueous layer was extracted twice with ethyl acetate, the

combined organic extracts were washed with brine, dried over anhydrous sodium sulfate and concentrated. The residue was chromatographed on silica gel with hexane-ethyl acetate (gradient 10:1, 4:1, 3: 1) to afford 58 mg (95%) of cannabidiol glycolate (ALL00149) as an oil.

[00209] For ALLOO 149, the ¹ H NMR (400 MHz, CDCl ₃ ) was as follows: δ= 6.59(1H, br s, ArH); 6.43(1H, d, J=1.8, ArH); 6.03(1H, br s, OH); 5.53(1H, br s, H-2); 4.59-4.62(1H, m); 4.44QH, br s); 4.30-4.40(2H, m, OCH ₂ CO ₂ ); 3.46(1 H, br s, H-3); 2.48-2.54(2H, m, benzylic CH ₂ ); 2.4O-2.47(1H, m); 2.39(2H, t, J=5.6, OH); 2.15-2.29(1H, m); 2.04-2.14(1H, m); 1.67- 1.86(2H, m); 1.78(3H, br s, 7-Me); 1.53-1.63(2H, m); 1.57(3H, br s, 10-Me); 1.23-1.37(4H, m); 0.88(3H, t, J=7.0, CH ₂ CH ₃ ).

[00210] 4.12 Synthesis ALLOOl 51 (CBD L-lactate)

[00211] To a stirred solution of cannabidiol (220.1 mg, 0.7 mmol) and L-(t- butyldimethylsilyl)lactic acid (299.4 mg, 1.26 mmol) in dry dichloromethane (2 mL) was added 4-dimethylaminopyridine (14.4 mg, 0.07 mmol) followed by N,N'-dicyclohexylcarbodiimide (310.5 mg, 1.505 mmol). The mixture was stirred at ambient temperature for 1 h. Additional amounts of the acid (45 mg) and N,N'-dicyclohexylcarbodiimide (60 mg) were added and stirring was continued for 1.5 h. The mixture was diluted with hexane (3 mL), filtered, and concentrated. The crude product was purified by preparative reverse phase HPLC (C8 column) with acetonitrile-water to afford cannabidiol L-(t-butyldimethylsilyl)lactate as an oil and cannabidiol bis(L-(t-butyldimethylsilyl))lactate (an intermediate for cannabidiol di(L-lactate)).

[00212] Cannabidiol L-(t-butyldimethylsilyl)lactate (15.1 mg) was dissolved in dry dichloromethane (0.1 mL), cooled to -15°C and treated with 0.1 mL of cold 2M solution of triethylamine trihydrofluoride in dichloromethane. The reaction mixture was left at 5 ⁰ C for 65 h. The mixture was poured to an excess of aqueous saturated sodium bicarbonate/ethyl acetate cooled to 0 ⁰ C with vigorous stirring. The aqueous layer was extracted twice with ethyl acetate. The combined organic extracts were washed with brine, dried over anhydrous sodium sulfate and concentrated. The residue was chromatographed on silica gel with hexane-ethyl acetate (gradient 10:1, 6:1, 4: 1) to afford 9.8 mg (82 %) of CBD L-lactate (ALL00151) as an oil.

[00213] For ALL00151, the ¹ H NMR (400 MHz, CDCl ₃ ) was as follows: δ=6.59(lH, br s, ArH); 6.37(1H, d, J=1.8, ArH); 6.02(1H, br s, OH); 5.52(1H, br s, H-2); 4.6O-4.63(1H, m); 4.44- 4.52(1H, m, OCHCO ₂ ); 4.44(1H, br s); 3.41(1H, br s, H-3); 2.77(1H, d, J=5.4, OH); 2.47-

2.53(2H, m, benzylic CH ₂ ); 2.4O-2.48(1H, m); 2.15-2.29(1H, m); 2.03-2.14(1H, m); 1.67- 1.86(2H, m); 1.78(3H, br s, 7-Me); 1.53-1.64(5H, m, aliphatic H and OCH(CH ₃ )CO ₂ ); 1.59(3H, br s, 10-Me); 1.23-1.38(4H, m); 0.88(3H, t, J=7.0, CH ₂ CH ₃ ).

[00214] 4.13 Proposed CBD-Hyaluronic acid

[00215] One or both of the hydroxyl groups on the cannabidiol molecule can be functionalized with various substituents (e.g., hyaluronic acid, lactic acid and glycolic acid) and optionally combined with dermatologically acceptable vehicles to form a cosmetic composition for application to the skin of mammal, such as a human. The scheme below represents a possible method for attachment of cannabidiol to hyaluronic acid using carbonate linkage and the TBDMS ether as a protective group. It may be anticipated that most of the primary hydroxyl group of the polymer would be involved in the formation of the linkage to the drug. Additional and/or different sets of protective group/deprotection methods could be used.

[00216] Also, glycolic acid and lactic acid could be attached to either or cannabidiol hydroxyl groups through an ester linkage or with the carbonate linkage by use of the 2-hydroxy group of acids. Cannabidiol could be also attached in a similar manner to a polymeric compound, such as hyaluronic acid. Using an additional spacer (e.g. 2-aminoethanol or glycine), the attachment in all cases could also be achieved through a carbamate linkage.

[00217] One of skill in the art would also recognize that, for example, cannabidiol may be attached to carboxylic groups of + acid through the ester linkage or through a carbamate linkage through the use of an additional spacer (e.g. 2-aminoethanol). In a further embodiment, an additional spacer may also be used when attaching cannabidiol to hydroxyl groups of hyaluronic acid.

[00218] 5.0 Plasma stability studies

[00219] An approximated 1 mg/mL stock solution of each prodrug was prepared in 100 μL of ethanol and 900 μL of acetonitrile. Ten (10) μ L of stock was spiked into 1 niL of plasma and vortexed. The samples were kept sealed in an amber vial and samples were obtained to analyze for bioconversion to parent drug.

[00220] 6.0 In vitro skin permeation studies

[00221] 6.1 Preparation of receiver fluid 1

[00222] One ( 1) L of receiver fluid was prepared by measuring 1 L of nanopure water into a graduated cylinder. 90% of the water was added to an Erlenmeyer flask. Hanks' salts (1 bottle) were added to the water along with 5.96 g of HEPES and 0.35 g of sodium bicarbonate. The pH of the solution was adjusted with 1 N sodium hydroxide solution to pH 7.4. The remaining water was added and the receiver fluid was filtered through a 0.2 μ filter (Millipore, Billerica, MA). Fifty (50) mg of gentamicin was added to the filtered receiver fluid and 400 mL of the receiver fluid was removed and replaced with 400 mL of PEG 400.

[00223] 6.2 Preparation of receiver fluid 2

[00224] The receiver fluid was prepared by measuring 600 mL of nanopure H ₂ O into a graduated cylinder. The H ₂ O was filtered through a 0.2 μ filter (Millipore, Billerica, MA). Fifty (50) mg of gentamicin was added to the filtered H ₂ O and 400 mL of PEG 400 was added.

[00225] 6.3.1 Preparation of drug formulation (Example 2)

[00226] The prodrugs were made up in a solution of 45:4: 1 PG:H ₂ O:IPM. For this solution, the appropriate amount of drug was weighed into a glass silanized culture tube and IPM was added, the 50 μL of PG to coat the drug, then an additional 247 μL PG was added and the donor solution was vortexed again. Finally 26 μ L of water was added. The gel formulation resulted from the mixing of absolute ethanol, nanopure water, IPM, Carbopol ^® 980, 0.1 N sodium hydroxide solution and the respective drug.

[00227] 6.3.2 Preparation of drug formulation (Example 2A)

[00228] The gel formulation was comprised of absolute ethanol, nanopure water, IPM, Carbopol® 980, 0.1 sodium hydroxide solution and respective drug. The anhydrous gel was

comprised of absolute ethanol, PEG monoethyl ether 550, Klucel® hydroxypropylcellulose, and respective drug.

[00229] 6.4 Permeation experiments

[00230] Dermatomed skin harvested from abdominoplasty, stored at -20 ⁰ C, was used for the experiments. A PermeGear flow-through (In-Line, Hellertown, PA) diffusion cell system was used for the skin permeation studies.

[00231] Diffusion cells were kept at 32°C with a circulating water bath. Human epidermal skin was arranged in the diffusion cell with stratum corneum (upper layer of skin) facing the donor compartment. The permeation area of the skin was 0.95 cm ² . The data was collected from a human skin donor with three diffusion cells per treatment.

[00232] The receiver solution was HEPES -buffered Hanks' balanced salts with gentamicin containing 40% PEG 400 at a pH of 7.4 or 40% aqueous PEG 400 and flow rate was adjusted to 0.8 mL/h. Each cell was charged with 0.10 mL of the respective drug formulation (donor solution) or 50 μ L of gel formulation which was rubbed into the skin for 15 sec with a Teflon coated rod. The formulation was applied to ensure complete coverage. The formulation was applied to ensure complete coverage. Diffusion cells were covered with a stopper or cap for the duration of the study.

[00233] Samples were collected into scintillation vials in 3 h increments for either 24, 30, or 42 h. All the samples were stored at 4°C until extracted. An aliquot (0.5 mL) of the diffusion sample was placed into a silanized HPLC vial and 0.5 mL of acetonitrile was added to the sample, capped and vortexed.

[00234] At the end of the experiment, the skin tissue was removed from the diffusion cell, rinsed with nanopure water, and blotted dry with a paper towel. The skin was tape stripped twice using book tape (Scotch™, 3M, St. Paul, MN) to remove drug formulation adhering to the tissue surface. The area of skin in contact with the drug was cut out, chopped up and placed in a pre- weighed scintillation vial. Ten (10) mL of acetonitrile was added to the vial and drug was extracted from the skin by shaking at room temperature overnight. The following day a 1 mL aliquot was removed transferred into a silanized HPLC vial for analysis. At the end of the experiments dosed with a gel, the skin tissue was rinsed 3 times with nanopure water for 10 s

each and wiped off with an alcohol pad. The entire piece of skin was blotted dry and tape stripped twice using book tape 845 (Scotch , 3M, St. Paul, MN) to remove any drug formulation adhering to the surface. The skin was rinsed an additional time with nanopure water and blotted dry again. The area of skin in contact with the drug was removed, minced with a scalpel, and placed in a pre-weighed scintillation vial. Drug was extracted from the area of skin in contact with the drug by equilibrating with 10 mL of ACN while shaking in a water bath overnight.

[00235] At the end of the experiment, a 0.01 mL aliquot of the donor solution was removed and added to a scintillation vial containing 10 mL of acetonitrile. The vials were vortexed and an aliquot of 1 mL was removed and transferred into a silanized HPLC vial for analysis. At the end of the experiments dosed with a gel, the donor compartment was rinsed 5 times with a known amount of nanopure water or ACN and then an aliquot of the nanopure water or ACN were diluted with ACN. The vials were vortexed and an aliquot of 1 mL was removed and transferred into a silanized HPLC vial for analysis.

[00236] 7.0.1 Analytical method (Example 2)

[00237] 7.0.2 Analytical method (Example 2A)

[00238] 7.0.3 Analytical method (Example 2B)

[00239] 8.0 Data analysis

[00240] The cumulative quantity of drug collected in the receiver compartment was plotted as a function of time. The flux value for a given experiment was obtained from the slope of a steady-state portion of the cumulative amount of drug permeated vs. time plot. Lag time was obtained from the x-intercept of the steady state portion of the cumulative amount of drug permeated vs. time plot. In Tables 16-19 and 21-23, the combined results of the delivered prodrug and cannabidiol from the prodrug are listed as "total cannabidiol." These values represent the data as total cannabidiol equivalents delivered in the form of cannabidiol and/or prodrug.

[00241] SECTION III. RESULTS

[00242] The cannabidiol prodrugs synthesized in Example 2 were all oily compounds.

[00243] In gel formulation, ALLOOlOl permeated through the skin as mono-substituted prodrug (ALL00150) only. ALLOOlOl did not increase the flux compared to cannabidiol. ALLOOlOl was detected in the skin as trace small amounts of intact prodrug but mostly as mono- substituted prodrug, ALLOO 150, and cannabidiol. Total skin concentrations were higher compared to cannabidiol. Lag time was decreased compared to cannabidiol.

[00244] Li donor solution, ALLOO 105 permeated through the skin as mono-substituted prodrug (ALL00148) and cannabidiol. ALL00105 did not increase the flux compared to cannabidiol. ALL00105 was detected in the skin as mono-substituted prodrug, ALL00148, and

cannabidiol. Total skin concentrations were higher compared to cannabidiol. Lag time was not decreased compared to cannabidiol.

[00245] In donor solution, ALLOOl 31 permeated through the skin as intact prodrug and cannabidiol. ALL00131 did not increase the flux compared to cannabidiol. ALL00131 was detected in the skin only as prodrug. Total skin concentrations were not higher compared to cannabidiol. Lag time was similar to cannabidiol.

[00246] In donor solution, ALLOO 132 permeated through the skin as intact prodrug, mono- substituted prodrug (ALL00131), and cannabidiol. ALL00132 did not increase the flux compared to cannabidiol. ALLOO 132 was detected in the skin as prodrug and small amounts as mono-substituted prodrug, ALL00131. Total skin concentrations were not higher compared to cannabidiol. Lag time wasn't decreased compared to cannabidiol.

[00247] In donor solution, ALLOOl 37 did not permeate through the skin or was below detection in the receiver samples. ALLOO 137 was detected in the skin as prodrug and trace amounts of cannabidiol. Total skin concentrations were not higher compared to cannabidiol.

[00248] In donor solution, ALLOO 140 permeated through the skin as intact prodrug and cannabidiol. ALL00140 did not increase the flux compared to cannabidiol. ALL00140 was detected mostly as prodrug in the skin with small amounts of cannabidiol. Total skin concentrations were not higher compared to cannabidiol. Lag time was slightly decreased compared to cannabidiol.

[00249] In donor solution, ALLOO 142 did not permeate through the skin or was below detection in the receiver samples. ALLOO 142 was detected in the skin only as intact prodrug. Total skin concentrations were not higher compared to cannabidiol.

[00250] In donor solution, ALLOO 143 did not permeate through the skin or was below detection in the receiver samples. ALL00143 was detected in the skin as prodrug and trace amounts of cannabidiol. Total skin concentrations were not higher compared to cannabidiol.

[00251] In donor solution, ALLOO 145 permeated through the skin as approximately 50% mono-substituted prodrug (ALL00146) and approximately 50% cannabidiol. ALL00145 did not increase the flux compared to cannabidiol. ALLOO 145 was detected in the skin as prodrug,

mono-substituted prodrug, ALLOO 146, and cannabidiol. Total skin concentrations were higher compared to cannabidiol. Lag time wasn't decreased compared to cannabidiol.

[00252] In gel formulation, ALLOO 146 permeated through the skin through the skin as intact prodrug and cannabidiol. ALLOO 146 did slightly increase the flux compared to cannabidiol. ALL00146 was detected in the skin as intact prodrug and cannabidiol. Total skin concentrations were not higher compared to cannabidiol. Lag time was similar to cannabidiol.

[00253] In donor solution, ALLOO 147 permeated through the skin as intact prodrug, mono- substituted prodrug (ALL00149), and cannabidiol. ALL00147 did not increase the flux compared to cannabidiol. ALLOO 147 was detected in the skin as prodrug, mono-substituted prodrug, ALL00149, and cannabidiol. Total skin concentrations were not higher compared to cannabidiol. Lag time was not decreased compared to cannabidiol.

[00254] In gel formulation, ALLOO 148 permeated through the skin through the skin as intact prodrug only. ALL00148 did not increase the flux compared to cannabidiol. ALL00148 was detected in the skin as mostly intact prodrug and small amounts of cannabidiol. Total skin concentrations were higher compared to cannabidiol. Lag time was similar to cannabidiol.

[00255] Decreased lag time may benefit patients by delivering the drug more quickly which is beneficial for pain management and nausea. Long lag times are more useful for topical and follicular therapies where drug absorption is attenuated. Cannabidiol prodrugs may increase permeation more if drug solution solvent systems were optimized or if they were formulated with enhancers. Many of these prodrugs may be useful in targeting topical/follicular applications instead of transdermal applications. By targeting topical administration, drug delivery could focus on a localized delivery compared to a systemic delivery. Also, follicular delivery may be targeted for administration with or without microparticle formulations.

[00256] In gel formulation, ALLOO 146 permeated through the skin as cannabidiol only, no prodrug was detected in the receiver fluid. ALL00146 didn't increase the flux compared to cannabidiol. ALLOO 146 was detected in the skin as small amount of cannabidiol with the remaining being prodrug. Total skin concentrations were slightly higher compared to cannabidiol. Lag time was higher compared to cannabidiol.

[00257] In gel formulation, ALLOO 150 did not permeate through the skin. ALLOO 150 was detected in the skin as primarily ALLOO 150 with a small amount of cannabidiol. Total skin concentrations were higher compared to cannabidiol.

[00258] In an anhydrous gel formulation, ALLOO 150 did not permeate through the skin. ALLOO 150 was detected in the skin as ALLOO 150 and cannabidiol. Total skin concentrations were higher compared to cannabidiol.

[00259] Overall, concentrations of cannabidiol and ALLOO 150 in the skin treated with the anhydrous gel formulation were much lower compared to the ethanol based gel formulation.

[00260] In gel formulation for cannabidiol, ALLOO 147, and ALLOO 149 only one cell of each drug had detectable levels in the receiver fluid of the respective drug. However all three cells of each drug had detectable skin concentrations. Therefore the diffusion data reported here reflects the data collected.

[00261] ALL00147 permeated through the skin as cannabidiol (47.2%) and mono-prodrug, ALL00149 (45.6%) with a trace amount of ALL00147 (7.2%). ALL00147 did increase the flux compared to cannabidiol by 2 fold. ALLOO 147 was detected in the skin primarily as mono- prodrug, ALL00149 with a trace amount of ALL00147. Total skin concentrations weren't higher compared to cannabidiol.

[00262] ALLOO 149 permeated through the skin as primarily ALLOO 149 with a trace amount of cannabidiol. ALL00149 did increase the flux compared to cannabidiol by 1.4. ALL00149 was detected in the skin as primarily ALL00149 with a trace amount of cannabidiol. Total skin concentrations were higher compared to cannabidiol. Lag time of ALLOO 149 was lower compared to cannabidiol.

[00263] SECTION IV. TABLES

[00264] Table 15. Cannabidiol and cannabidiol prodrugs

^* LogP calculated by ChemSketch 10.02 (Advanced Chemistry Development, Inc; Canada)

[00265] Table 16. Permeation data of CBD (n=3), ALL00131 (n=3), ALL00132 (n=2), and ALL00140 (n=3) in 90:8:2 PG:H2O:IPM donor solution with 60/40 Hanks'/PEG 400 receiver fluid

* total CBD= total cannabidiol equivalents delivered in the form of cannabidiol and/or prodrug

Wherein "n" equals the number of skin samples tested.

[00266] Table 17. Permeation data of CBD (n=3), ALL00137 (n=3), ALL00142 (n=3), and ALL00143 (n=3) in 90:8:2 PG:H2O:IPM donor solution with 40% aqueous PEG 400 receiver fluid

* total CBD= total cannabidiol equivalents delivered in the form of cannabidiol and/or prodrug Wherein "n" equals the number of skin samples tested.

[00267] Table 18. Permeation data of CBD (n=3), ALL00105 (n=l) ALL00145 (n=3), and ALL00147 (n=2) in 90:8:2 PG:H2O:IPM donor solution with 40% aqueous PEG 400 receiver fluid

^* total CBD= total cannabidiol equivalents delivered in the form of cannabidiol and/or prodrug Wherein "n" equals the number of skin samples tested.

[00268] Table 19. Permeation data of CBD (n=2), ALLOOlOl (n=2) ALL00146 (n=2), and ALL00148 (n=3) in gel formulation with 40% aqueous PEG 400 receiver fluid

^* total CBD= total cannabidiol equivalents delivered in the form of cannabidiol and/or prodrug Wherein "n" equals the number of skin samples tested.

[00269] Table 20. Plasma stability of cannabidiol prodrugs

[00270] Table 21. Permeation data of CBD (n=2), ALL00146 (n=2), and ALL00150 (n=3) in gel formulation with 40% aqueous PEG 400 receiver fluid.

total CBD= total cannabidiol equivalents delivered in the form of cannabidiol and/or prodrug Wherein "n" equals the number of skin samples tested.

[00271] Table 22. Permeation data of CBD (n=2) and ALL00150 (n=3) in an anhydrous gel formulation with 40% aqueous PEG 400 receiver fluid.

^* total CBD= total cannabidiol equivalents delivered in the form of cannabidiol and/or prodrug Wherein "n" equals the number of skin samples tested.

[00272] Table 23. Permeation data of CBD (n=l), ALL00146 (n=l), and ALL00150 (n=l) in gel formulation with 40% aqueous PEG 400 receiver fluid.

^* total CBD= total cannabidiol equivalents delivered in the form of cannabidiol and/or prodrug [00273] Table 24. Plasma stability of cannabidiol prodrugs

[00274] All references, including publications, patent applications, and patents, cited herein are hereby incorporated by reference to the same extent as if each reference were individually and specifically indicated to be incorporated by reference and were set forth in its entirety herein.

[00275] The use of the terms "a," "an" and "the" and similar references in the context of this disclosure (especially in the context of the following claims) are to be construed to cover both

the singular and the plural, unless otherwise indicated herein or clearly contradicted by context. All methods described herein can be performed in any suitable order unless otherwise indicated herein or otherwise clearly contradicted by context. The use of any and all examples, or exemplary language (e.g., such as, preferred, preferably) provided herein, is intended merely to further illustrate the content of the disclosure and does not pose a limitation on the scope of the claims. No language in the specification should be construed as indicating any non-claimed element as essential to the practice of the present disclosure.

[00276] Alternative embodiments of the claimed disclosure are described herein, including the best mode known to the inventors for practicing the claimed invention. Of these, variations of the disclosed embodiments will become apparent to those of ordinary skill in the art upon reading the foregoing disclosure. The inventors expect skilled artisans to employ such variations as appropriate (e.g., altering or combining features or embodiments), and the inventors intend for the invention to be practiced otherwise than as specifically described herein.

[00277] Accordingly, this invention includes all modifications and equivalents of the subject matter recited in the claims appended hereto as permitted by applicable law. Moreover, any combination of the above described elements in all possible variations thereof is encompassed by the invention unless otherwise indicated herein or otherwise clearly contradicted by context.

[00278] The use of individual numerical values are stated as approximations as though the values were preceded by the word "about" or "approximately." Similarly, the numerical values in the various ranges specified in this application, unless expressly indicated otherwise, are stated as approximations as though the minimum and maximum values within the stated ranges were both preceded by the word "about" or "approximately." In this manner, variations above and below the stated ranges can be used to achieve substantially the same results as values within the ranges. As used herein, the terms "about" and "approximately" when referring to a numerical value shall have their plain and ordinary meanings to a person of ordinary skill in the art to which the disclosed subject matter is most closely related or the art relevant to the range or element at issue. The amount of broadening from the strict numerical boundary depends upon many factors. For example, some of the factors which may be considered include the criticality of the element and/or the effect a given amount of variation will have on the performance of the claimed subject matter, as well as other considerations known to those of skill in the art. As used

herein, the use of differing amounts of significant digits for different numerical values is not meant to limit how the use of the words "about" or "approximately" will serve to broaden a particular numerical value or range. Thus, as a general matter, "about" or "approximately" broaden the numerical value. Also, the disclosure of ranges is intended as a continuous range including every value between the minimum and maximum values plus the broadening of the range afforded by the use of the term "about" or "approximately." Thus, recitation of ranges of values herein are merely intended to serve as a shorthand method of referring individually to each separate value falling within the range, unless otherwise indicated herein, and each separate value is incorporated into the specification as if it were individually recited herein.

[00279] It is to be understood that any ranges, ratios and ranges of ratios that can be formed by, or derived from, any of the data disclosed herein represent further embodiments of the present disclosure and are included as part of the disclosure as though they were explicitly set forth. This includes ranges that can be formed that do or do not include a finite upper and/or lower boundary. Accordingly, a person of ordinary skill in the art most closely related to a particular range, ratio or range of ratios will appreciate that such values are unambiguously derivable from the data presented herein.