Title:
PRODUCTION METHOD OF EXTRACELLULAR VESICLE POPULATION, AND EXTRACELLULAR VESICLE POPULATION
Document Type and Number:
WIPO Patent Application WO/2023/200002
Kind Code:
A1
Abstract:
The present invention provides a production method of an extracellular vesicle population containing extracellular vesicles on the membrane surface of which phosphatidylserine is low expressed, said method comprising: (1) a step for culturing mesenchymal stem cells in a medium containing one or more sulfuric acid compounds selected from the group consisting of a sulfated glycolipid, cholesterol sulfate and pharmaceutically acceptable salts thereof; and (2) a step for collecting an extracellular vesicle population contained in the medium after carrying out the above step (1). The present invention also provides an extracellular vesicle population containing extracellular vesicles on the membrane surface of which phosphatidylserine is low expressed.
Inventors:
YAMASHITA TATSUYUKI (JP)
HONKE KOICHI (JP)
HONKE KOICHI (JP)
Application Number:
PCT/JP2023/015157
Publication Date:
October 19, 2023
Filing Date:
April 14, 2023
Export Citation:
Assignee:
UNIV NAT CORP KOCHI UNIV (JP)
International Classes:
C12N5/0775; A61K35/12; A61K35/28; A61P9/10; A61P25/00; C12N5/071
Foreign References:
| JP2020514400A | 2020-05-21 |
Other References:
YAMASHITA, TATSUYUKI : "P-01-023: Effect of sulfated glycolipid sulfatide on exosomal secretion of mesenchymal stem cells. ", REGENERATIVE MEDICINE, JAPANESE SOCIETY FOR REGENERATIVE MEDICINE, JP, vol. 16, no. suppl., 1 January 2017 (2017-01-01), JP , pages 367, XP009549508, ISSN: 1347-7919
YAMASHITA, TATSUYUKI: "Sulfated glycolipid seminolipids promote exosome secretion of Sertoli cells.", PROCEEDINGS OF THE 35TH ANNUAL MEETING OF THE JAPANESE SOCIETY OF CARBOHYDRATE RESEARCH, 1 January 2016 (2016-01-01), pages 189 (P - 091), XP009549507
MOT YEE YIK, MOSES EMMANUEL JAIRAJ, MOHD YUSOFF NARAZAH, LING KING-HWA, YONG YOKE KEONG, TAN JUN JIE: "Mesenchymal Stromal Cells-Derived Exosome and the Roles in the Treatment of Traumatic Brain Injury", CELLULAR AND MOLECULAR NEUROBIOLOGY, SPRINGER NEW YORK, US, vol. 43, no. 2, 1 March 2023 (2023-03-01), US , pages 469 - 489, XP093098753, ISSN: 0272-4340, DOI: 10.1007/s10571-022-01201-y
YAMASHITA, TATSUYUKI: "Sulfated glycolipid seminolipids promote exosome secretion of Sertoli cells.", PROCEEDINGS OF THE 35TH ANNUAL MEETING OF THE JAPANESE SOCIETY OF CARBOHYDRATE RESEARCH, 1 January 2016 (2016-01-01), pages 189 (P - 091), XP009549507
MOT YEE YIK, MOSES EMMANUEL JAIRAJ, MOHD YUSOFF NARAZAH, LING KING-HWA, YONG YOKE KEONG, TAN JUN JIE: "Mesenchymal Stromal Cells-Derived Exosome and the Roles in the Treatment of Traumatic Brain Injury", CELLULAR AND MOLECULAR NEUROBIOLOGY, SPRINGER NEW YORK, US, vol. 43, no. 2, 1 March 2023 (2023-03-01), US , pages 469 - 489, XP093098753, ISSN: 0272-4340, DOI: 10.1007/s10571-022-01201-y
Attorney, Agent or Firm:
AOKI, Atsushi et al. (JP)
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