Title:
PRODUCTION METHOD FOR PARASYMPATHETIC NERVE CELLS
Document Type and Number:
WIPO Patent Application WO/2020/040286
Kind Code:
A1
Abstract:
Provided is a method for producing parasympathetic nerve cells from neural crest cells or autonomic nerve precursor cells derived from neural crest cells, the method comprising a step for culturing neural crest cells or autonomic nerve precursor cells derived therefrom in the presence of a cAMP production accelerator, a BDNF signaling pathway activator, a GDNF signaling pathway activator, an NGF signaling pathway activator, an NT-3 signaling pathway activator, vitamin C, a protein kinase C activator, and a retinoic acid receptor agonist.
Inventors:
TAKAYAMA YUZO (JP)
KIDA YASUYUKI (JP)
KIDA YASUYUKI (JP)
Application Number:
PCT/JP2019/033019
Publication Date:
February 27, 2020
Filing Date:
August 23, 2019
Export Citation:
Assignee:
AIST (JP)
International Classes:
C12N5/0793; C12N5/0797
Domestic Patent References:
| WO2016194522A1 | 2016-12-08 | |||
| WO2018218193A1 | 2018-11-29 | |||
| WO2016194522A1 | 2016-12-08 |
Other References:
TAKAYAMA, YUZO ET AL.: "TC6-10 Induction of autonomic nerve cell from human pluripotent stem cells and its application", LECTURE PROCEEDINGS OF THE 2017 ANNUAL CONFERENCE OF ELECTRONICS, vol. 29, 2017, pages 223 - 228, XP009525966
BERRARD S; BIGUET N FAUCON; HOUHOU L; LAMOUROUX A; MALLET J: "Retinoic acid induces cholinergic differentiation of cultured newborn rat sympathetic neurons.", JOURNAL OF NEUROSCIENCE RESEARCH, vol. 35, no. 4, 1 July 1993 (1993-07-01), pages 382 - 389, XP055687457, ISSN: 0360-4012, DOI: 10.1002/jnr.490350405
YUZO TAKAYAMA; WAKABAYASHI TAMAMI; KUSHIGE HIROKO; SAITO YUTAKA; SHIBUYA YOICHIRO; SHIBATA SHINSUKE; AKAMATSU WADO; OKANO HIDEYUKI: "Brief exposure to small molecules allows induction of mouse embryonic fibroblasts into neural crest-like precursors", FEBS LETTERS, vol. 591, no. 4, 9 February 2017 (2017-02-09), pages 590 - 602, XP055687460, ISSN: 0014-5793, DOI: 10.1002/1873-3468.12572
MIZUNO, KENICHI ET AL.: "Intracellular factors involved in cholinergic neuronal differentiation", BULLETIN OF THE JAPANESE NEUROCHEMICAL SOCIETY, vol. 28, no. 1, 1989, pages 86 - 87, XP009525940
M. YAMASHITA ET AL.: "Identification of a conserved GATA3 response element upstream proximal from the Interleukin-13 gene locus", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 277, 2002, pages 42399 - 42408, XP002971059, DOI: 10.1074/jbc.M205876200
D. NONAKA ET AL.: "A study of Gata3 and Phox2b expression in tumors of the autonomic nervous system", THE AMERICAN JOURNAL OF SURGICAL PATHOLOGY, vol. 37, 2013, pages 1236 - 1241
M. KOBAYASHI ET AL.: "Cholinergic differentiation of cultured sympathetic neurons induced by retinoic acid. Induction of choline acetyltransferase-mRNA and suppression of tyrosine hydroxylase-mRNA levels", FEBS LETTERS, vol. 337, 1994, pages 259 - 264
OH ET AL., CELL STEM CELL, vol. 19, 2016, pages 95 - 106
FUKUTA ET AL., PLOS ONE, vol. 9, no. 12, 2014, pages el 12291
MIZUSEKI ET AL., PROC. NATL. ACAD. SCI. USA, vol. 100, 2003, pages 5828 - 5833
See also references of EP 3825397A4
BERRARD S; BIGUET N FAUCON; HOUHOU L; LAMOUROUX A; MALLET J: "Retinoic acid induces cholinergic differentiation of cultured newborn rat sympathetic neurons.", JOURNAL OF NEUROSCIENCE RESEARCH, vol. 35, no. 4, 1 July 1993 (1993-07-01), pages 382 - 389, XP055687457, ISSN: 0360-4012, DOI: 10.1002/jnr.490350405
YUZO TAKAYAMA; WAKABAYASHI TAMAMI; KUSHIGE HIROKO; SAITO YUTAKA; SHIBUYA YOICHIRO; SHIBATA SHINSUKE; AKAMATSU WADO; OKANO HIDEYUKI: "Brief exposure to small molecules allows induction of mouse embryonic fibroblasts into neural crest-like precursors", FEBS LETTERS, vol. 591, no. 4, 9 February 2017 (2017-02-09), pages 590 - 602, XP055687460, ISSN: 0014-5793, DOI: 10.1002/1873-3468.12572
MIZUNO, KENICHI ET AL.: "Intracellular factors involved in cholinergic neuronal differentiation", BULLETIN OF THE JAPANESE NEUROCHEMICAL SOCIETY, vol. 28, no. 1, 1989, pages 86 - 87, XP009525940
M. YAMASHITA ET AL.: "Identification of a conserved GATA3 response element upstream proximal from the Interleukin-13 gene locus", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 277, 2002, pages 42399 - 42408, XP002971059, DOI: 10.1074/jbc.M205876200
D. NONAKA ET AL.: "A study of Gata3 and Phox2b expression in tumors of the autonomic nervous system", THE AMERICAN JOURNAL OF SURGICAL PATHOLOGY, vol. 37, 2013, pages 1236 - 1241
M. KOBAYASHI ET AL.: "Cholinergic differentiation of cultured sympathetic neurons induced by retinoic acid. Induction of choline acetyltransferase-mRNA and suppression of tyrosine hydroxylase-mRNA levels", FEBS LETTERS, vol. 337, 1994, pages 259 - 264
OH ET AL., CELL STEM CELL, vol. 19, 2016, pages 95 - 106
FUKUTA ET AL., PLOS ONE, vol. 9, no. 12, 2014, pages el 12291
MIZUSEKI ET AL., PROC. NATL. ACAD. SCI. USA, vol. 100, 2003, pages 5828 - 5833
See also references of EP 3825397A4
Attorney, Agent or Firm:
OKUYAMA, Shoichi et al. (JP)
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