Title:
PROTEIN-IMMOBILIZING SOLID PHASE, POLYNUCLEOTIDE-IMMOBILIZING SOLID PHASE, AND NUCLEIC ACID RECOVERY METHOD
Document Type and Number:
WIPO Patent Application WO/2013/065782
Kind Code:
A1
Abstract:
The invention provides a protein-immobilizing solid phase and a polynucleotide-immobilizing solid phase that make it possible to recover nucleic acid efficiently from the solid phase, and an efficient nucleic acid recovery method. In one embodiment of the invention, the protein-immobilizing solid phase is obtained by immobilizing, on a solid phase, an mRNA(23)-nucleic acid linker (2)-protein (33) complex obtained by linking mRNA (23) and a protein (33) encoded by this mRNA (23) via a nucleic acid linker (2), the protein-immobilizing solid phase being characterized in that the nucleic acid linker (2) has a photocleavage site (2c) and a solid phase-binding site (2b).
Inventors:
UENO SHINGO (JP)
NEMOTO NAOTO (JP)
ICHIKI TAKANORI (JP)
SHIONO HIROFUMI (JP)
OSAWA HISAO (JP)
NEMOTO NAOTO (JP)
ICHIKI TAKANORI (JP)
SHIONO HIROFUMI (JP)
OSAWA HISAO (JP)
Application Number:
PCT/JP2012/078321
Publication Date:
May 10, 2013
Filing Date:
November 01, 2012
Export Citation:
Assignee:
UNIV TOKYO (JP)
NIKON CORP (JP)
NIKON CORP (JP)
International Classes:
C12N15/09; C07K17/14; C12M1/00
Foreign References:
| JP2011087573A | 2011-05-06 |
Other References:
YAMAGUCHI J. ET AL.: "cDNA display: a novel screening method for functional disulfide-rich peptides by solid-phase synthesis and stabilization of mRNA-protein fusions. art e108", NUCLEIC ACIDS RES., vol. 37, no. 16, 2009, pages 1 - 13, XP008154704
Attorney, Agent or Firm:
SHIGA Masatake et al. (JP)
Masatake Shiga (JP)
Masatake Shiga (JP)
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Claims: