Title:
PROTEIN-MAGNETIC PARTICLE COMPLEX, AND METHOD FOR PRODUCING SAME
Document Type and Number:
WIPO Patent Application WO/2015/087848
Kind Code:
A1
Abstract:
Method for producing a complex of magnetic particles and a disulfide-binding protein in a gram-negative magnetotactic bacterium, the method involving: a coexpression step (i) in which a first fusion protein (a) containing a disulfide-binding protein fused to a binding protein, and a second fusion protein (b) containing a binding partner protein fused to a protein that can bind or integrate with bacterial magnetic particles are coexpressed in a gram-negative magnetotactic bacterium so that the expression of the first fusion protein is induced to the periplasm of said bacterium and the expression of the second fusion protein is induced to the cytoplasm of the aforementioned bacterium; a culture step (ii) in which the bacterium of step (i) is cultured under conditions sufficient for the production of the first and second fusion proteins; and a cell membrane breakdown step (iii) in which the cell membrane of the bacterium of step (ii) is broken down to form a complex of the disulfide-binding protein and bacterial magnetic particles.
Inventors:
YOSHINO TOMOKO (JP)
TANAKA TSUYOSHI (JP)
MATSUNAGA TADASHI (JP)
SUGAMATA YASUHIRO (JP)
HONDA TORU (JP)
TANAKA TSUYOSHI (JP)
MATSUNAGA TADASHI (JP)
SUGAMATA YASUHIRO (JP)
HONDA TORU (JP)
Application Number:
PCT/JP2014/082469
Publication Date:
June 18, 2015
Filing Date:
December 08, 2014
Export Citation:
Assignee:
UNIV TOKYO NAT UNIV CORP (JP)
International Classes:
C12N11/02; C12N15/09
Foreign References:
| JP2012526130A | 2012-10-25 | |||
| JP2002543761A | 2002-12-24 | |||
| JP2002535004A | 2002-10-22 |
Other References:
TOMOKO YOSHINO ET AL.: "Design and engineering of protein-magnetic nanoparticle complexes by bacteria", BIOSCIENCE & INDUSTRY, vol. 71, no. 6, 1 November 2013 (2013-11-01), pages 508 - 511
TOMOKO YOSHINO ET AL.: "Biosynthesis of Functional Protein-Magnetic Nanoparticle Complexes with Microorganisms", OLEOSCIENCE, vol. 14, no. 10, 1 October 2014 (2014-10-01), pages 433 - 438
SUGAMATA, Y. ET AL.: "Functional expression of an scFv on bacterial magnetic particles by in vitro docking", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 445, 25 January 2014 (2014-01-25), pages 1 - 5
TOMOKO YOSHINO ET AL.: "Biosynthesis of Functional Protein-Magnetic Nanoparticle Complexes with Microorganisms", OLEOSCIENCE, vol. 14, no. 10, 1 October 2014 (2014-10-01), pages 433 - 438
SUGAMATA, Y. ET AL.: "Functional expression of an scFv on bacterial magnetic particles by in vitro docking", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 445, 25 January 2014 (2014-01-25), pages 1 - 5
Attorney, Agent or Firm:
ONO Makoto et al. (JP)
Makoto Ono (JP)
Makoto Ono (JP)
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