Title:
PROTEINASE K VARIANTS HAVING DECREASED ADSORPTION PROTEINASE K VARIANTS HAVING DECREASED ADSORPTION AND INCREASED HYDROLYSIS AND INCREASED HYDROLYSIS
Document Type and Number:
WIPO Patent Application WO1996028556
Kind Code:
A3
Abstract:
The present invention relates to Proteinase K variants having a modified amino acid sequence of wild-type Proteinase K amino acid sequence, the wild-type amino acid sequence comprising a first loop region, a second loop region, a third loop region, a fourth loop region, a fifth loop region and a sixth loop region; wherein the modified amino acid sequence comprises different amino acids than that occurring in wild-type Proteinase K (i.e., substitution) at specifically identified positions in one or more of the loop regions whereby the Proteinase K variant has decreased adsorption to, and increased hydrolysis of, an insoluble substrate as compared to the wild-type Proteinase K. The present invention also relates to DNA sequences encoding such Proteinase K variants. The present invention also relates to compositions comprising such Proteinase K variants for cleaning a variety of surfaces.
Inventors:
BRODE PHILIP FREDERICK III
BARNETT BOBBY LEE
RUBINGH DONN NELTON
BARNETT BOBBY LEE
RUBINGH DONN NELTON
Application Number:
PCT/US1996/003005
Publication Date:
July 17, 1997
Filing Date:
March 06, 1996
Export Citation:
Assignee:
PROCTER & GAMBLE (US)
International Classes:
A61K8/66; A61Q11/00; C11D3/00; C11D3/386; C12N15/09; C11D17/00; C12N9/54; C12N9/58; C12N15/57; (IPC1-7): C12N15/57; C12N9/64; C12N9/54; C11D9/40
Domestic Patent References:
| WO1989009819A1 | 1989-10-19 | |||
| WO1995030011A2 | 1995-11-09 | |||
| WO1995030010A1 | 1995-11-09 |
Foreign References:
| EP0405901A1 | 1991-01-02 |
Other References:
P. BRODE ET AL: "Surface active variants of subtilisin BPN': interfacial hydrolysis", JOURNAL OF CELLULAR BIOCHEMISTRY, vol. 18D, 1994, pages 151, XP000651912
SIEZEN R J ET AL: "HOMOLOGY MODELLING AND PROTEIN ENGINEERING STRATEGY OF SUBTILASES, THE FAMILY OF SUBTILISIN-LIKE SERINE PROTEINASES", PROTEIN ENGINEERING, vol. 4, no. 7, 1 January 1991 (1991-01-01), pages 719 - 737, XP002008733
CHEMICAL ABSTRACTS, vol. 116, no. 23, 8 June 1992, Columbus, Ohio, US; abstract no. 230623, BRODE, P. F., III ET AL: "Subtilisin BPN': activity on an immobilized substrate" XP002027110
SIEZEN R J ET AL: "HOMOLOGY MODELLING AND PROTEIN ENGINEERING STRATEGY OF SUBTILASES, THE FAMILY OF SUBTILISIN-LIKE SERINE PROTEINASES", PROTEIN ENGINEERING, vol. 4, no. 7, 1 January 1991 (1991-01-01), pages 719 - 737, XP002008733
CHEMICAL ABSTRACTS, vol. 116, no. 23, 8 June 1992, Columbus, Ohio, US; abstract no. 230623, BRODE, P. F., III ET AL: "Subtilisin BPN': activity on an immobilized substrate" XP002027110
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