Background of the Invention Many disease-causing microorganisms possess a neuraminidase (also known as sialidase) which is involved in the replication process of the microorganism. In particular, viruses of the orthomyxovirus and paramyxovirus groups possess a neuraminidase. Diseases associated with paramyxoviruses include RSV (respiratory syncytial virus-related diseases), pneumonia and bronchiolitis (associated with paramyxovirus type 3) and laryngotracheobronchitis (associated with paramyxovirus type 1). Some of the more important disease- causing microorganisms in man and/or animals which possess a neuraminidase include Vibrio cholerae, Clostridium perfringens, Streptococcus pneumoniae, Arthrobacter sialophilus, influenza virus, parainfluenza virus, mumps virus, Newcastle disease virus, fowl plague virus, equine influenza virus and Sendai virus.

Mortality due to influenza is a serious problem throughout the world. The disease is devastating to man, lower mammals and some birds. Although vaccines containing attenuated influenza virus are available, those vaccines only provide immunological protection toward a few influenza strains and are less effective in otherwise immunologically compromised populations such as the elderly, young children, and in those who suffer from chronic respiratory illness.

The productivity loss from absence due to sickness from influenza virus infection has been estimated to be more than $1 billion per year.

There are two major strains of influenza virus (designated A and B).

Currently, there are only a few pharmaceutical products approved for treating influenza. These include amantadine and rimantadine, which are active only against the A strain of influenza viruses, and ribavirin, which suffers from dose-limiting toxicity. Mutant virus which is resistant to amantadine and rimantadine emerges quickly during treatment with these agents.

Very recently the first influenza neuraminidase inhibitor, zanamivir, was approved. However, it can only be administered by inhalation. Therefore, there is a continuing need for improved agents for treatment and/or prevention of influenza infection.

Neuraminidase is one of two major viral proteins which protrude from the envelope of influenza virus. During the release of progeny virus from infected cells, neuraminidase cleaves terminal sialic acid residues from glycoproteins, glycolipids and oligosaccharides on the cell surface. Inhibition of neuraminidase enzymatic activity leads to aggregation of progeny virus at the surface. Such virus is incapable of infecting new cells, and viral replication is therefore retarded or blocked. X-ray crystallographic studies and sequence alignments have shown that the residues which directly contact the sialic acid portion of the substrate are strictly conserved in the neuraminidase from all A and B influenza strains. Thus, a compound which binds to the sialic acid binding region of the neuraminidase active site will block the replication of both the A and B strains of influenza virus.

Compounds which are influenza neuraminidase inhibitors will be useful for the prevention of influenza infection and will be useful for the treatment of influenza infection.

The following references disclose neuraminic acid derivatives with the disclosed utility listed after each reference: L. Von Itzstein, et al., European Patent Application No. EP539204, published April 28,1993 (antiviral agent); T. Honda, et al., European Patent Application No. EP823428, published February 11, 1998 (sialidase inhibitor ; influenza treatment); T. Honda, et al., International Patent Application No. W098/06712, published February 19, 1998 (sialidase inhibitor; influenza remedy); L. Von Itzstein, et al., International Patent Application No. W095/20583, published August 3, 1995 (viral neuraminidase inhibitor; influenza treatment); P. Smith, International Patent Application No. W095/18800, published July 13, 1995 (viral neuraminidase inhibitor); P. Colman, et al., International Patent Application No. W092/06691, published April 30,1992 (viral neuraminidase inhibitor); L. Von Itzstein, et al., U. S. Patent No. 5, 648, 379, issued July 15,1997 (influenza treatment) ; P. Reece, et al., International Patent Application No. W097/32214, published September 4,1997 (bind to influenza virus neuraminidase active site); and P. Reece, et al., International Patent Application No. W098/21243, published May 23, 1998 (anti-influenza agent).

The following references disclose sialic acid derivatives with the disclosed utility listed after each reference: Y. Ohira, et al., International Patent Application No. W098/11083, published March 19, 1998 (antiviral agent); Y. Ohira, European Patent Application No. EP882721, published December 9, 1998 (antiviral agent); and B. Glaner, et al., Helvetica Chimica Acta 74 343-369 (1991) (Vibrio cholerae neuraminidase inhibitor).

The following references disclose benzene derivatives, cyclohexane derivatives or cyclohexene derivatives with the disclosed utility listed after each reference: Y. Babu, et al., U. S. Patent No. 5, 602, 277, issued February 11,1997 (neuraminidase inhibitors); M. Luo, et al., U. S. Patent No. 5, 453,533, issued September 26, 1995 (influenza neuraminidase inhibitor; influenza treatment); Y. Babu, et al., International Patent Application No. W096/30329, published October 3,1996 (neuraminidase inhibitor; viral infection treatment); N. Bischofberger, et al., U. S. Patent No. 5, 763,483, issued June 9,1998 (neuraminidase inhibitor); C. Kim, et al., International Patent Application No. W099/31047, published June 24,1999 (neuraminidase inhibitor; influenza treatment); V. Atigadda, et al., J. Med. Chem. 42 2332-2343 (1999) (influenza neuraminidase inhibitor); and K. Kent, et al., International Patent Application No. 98/07685, published February 26,1998 (intermediates for the preparation of neuraminidase inhibitors).

C. Kim, et al., International Patent Application No. W098/17647, published April 30,1998 discloses piperidine derivatives that are useful as neuraminidase inhibitors.

N. Bischofberger, et al., International Patent Application No. W096/26933, published September 6,1996 and N. Bischofberger, et al., International Patent Application No. W099/14185, published March 25, 1999 disclose various substituted 6-membered ring compounds that are useful as neuraminidase inhibitors.

The following references disclose dihydropyran derivatives that are useful as viral neuraminidase inhibitors: D. Andrews, et al., International Patent Application No. W097/06157, published February 20,1997 and U. S. Patent No. 5, 919, 819, issued July 6,1999; and P. Cherry, et al., International Patent Application No. W096/36628, published November 21, 1996.

C. Kim, et al., U. S. Patent No. 5,512,596, issued April 30,1996 discloses 6-membered aromatic ring derivatives that are useful as neuraminidase inhibitors.

G. Diana, et al., International Patent Application No. W098/03487, published January 29,1998 discloses substituted pyridazines that are useful for treatment of influenza.

B. Horenstein, et al., International Patent Application No. W099/06369, published February 11,1999 discloses piperazine derivatives that are useful as neuraminidase inhibitors.

The following references disclose substituted cyclopentanes that are useful as neuraminidase inhibitors and treatments for influenza : Y. Babu, et al., International Patent Application No. W097/47194, published December 18,1997; and Y. Babu, et al., International Patent Application No. W099/33781, published July 8,1999.

L. Czollner, et al., Helvetica Chimica Acta 73 1338-1358 (1990) discloses pyrrolidine analogs of neuraminic acid that are useful as Vibrio cholerae sialidase inhibitors.

W. Brouillette, et al., International Patent Application No. W099/14191, published March 25, 1999, discloses substituted pyrrolidin-2-one compounds that are useful as neuraminidase inhibitors and treatments for influenza.

The following references disclose siastatin B analogs that are useful as neuraminidase inhibitors: Y. Nishimura, et al., Natural Product Letters 1 39-44 (1992); and Y. Nishimura, et al., Natural Product Letters 1 33-38 (1992).

C. Penn, UK Patent Application No. GB2292081, published February 14, 1996 discloses the use of a neuraminidase inhibitor in combination with an influenza vaccine.

An object of the invention is to provide compounds that inhibit neuraminidase of disease-causing microorganisms; especially, viral neuraminidase; and, most especially, influenza neuraminidase.

An object of the invention is also to provide compounds that inhibit neuraminidase from both A and B strains of influenza.

Another object of the invention is to provide prophylaxis of influenza infection in humans and other mammals.

Another object of the invention is to provide treatment of influenza infection in humans and other mammals.

Another object of the invention is to provide compounds that exhibit activity against influenza A virus and and influenza B virus by virtue of inhibiting influenza neuraminidase when such compounds are administered orally.

Another object of the invention is to provide a compound that can be effectively transported from the plasma into the lung bronchoaveolar fluid of humans and other mammals in order to block the replication of influenza virus in that tissue.

Disclosure of the Invention The present invention discloses compounds having Formula t : or a pharmaceutically acceptable salt, ester or prodrug thereof, wherein R'is selected from the group consisting of (a)-CO2H, (b)-CH2CO2H, (c)-S03H, (d)-CH2SO3H, (e)-SO2H, (f) -CH2SO2H, (g)-P03H2, (h)-CH2PO3H2, (i)-P02H, (j)-CH2PO2H, (k) tetrazolyl, (I)-CH2-tetrazolyl, (m)-C (=O)-NH-S (O) 2-R11, (n)-CH2C (=O)-NH-S (O) 2-R11, (o)-S02N (T-R") R12 and (p)-CH2SO2N (T-R") R'2 wherein T is selected from the group consisting of (i) a bond, (ii)-C (=O)-, (iii)-C (=O) O-, (iv)-C (=O) S-, (v)-C (=O) NR36-, (vi)-C (=S) O-, (vii)-C (=S) S-, and (viii)-C (=S) NR36-, R"is selected from the group consisting of (i) C1-C12alkyl, (ii) C2-Ci2 alkenyl, (iii) cycloalkyl, (iv) (cyclo- alkyl) alkyl, (v) (cycloalkyl) alkenyl, (vi) cycloalkenyl, (vii) (cycloalkenyl) alkyl, (viii) (cycloalkenyl) alkenyl, (ix) aryl, (x) (aryl) alkyl, (xi) (aryl) alkenyl, (xii) heterocyclic, (xiii) (heterocyclic) alkyl and (xiii) (xiv) (heterocyclic) alkenyl ; and R12 and R36 are independently selected from the group consisting of (i) hydrogen, (ii) C1-C12 alkyl, (iii) C2-C12 alkenyl, (iv) cycloalkyl, (v) (cycloalkyl) alkyl, (vi) (cycloalkyl) alkenyl, (vii) cycloalkenyl, (viii) (cycloalkenyl) alkyl, (ix) (cycloalkenyl) alkenyl, (x) aryl, (xi) (aryl) alkyl, (xii) (aryl) alkenyl, (xiii) heterocyclic, (xiv) (heterocyclic) alkyl and (xv) (heterocyclic) alkenyl ; X is selected from the group consisting of (a)-C (=O)-N (R*)-, (b)-N (R*)-C (=O)-, (c)-C (=S)-N (R*)-, (d)-N (R*)-C (=S)-, (e)-N (R*)-S02-, and (f)-SO2-N (R*)-wherein R* is hydrogen, C1-C3 loweralkyl or cyclopropyl ; R2 is selected from the group consisting of (a) hydrogen, (b) Ci-Ce alkyl, (c) C2-C6 alkenyl, (d) C3-C6 cycloalkyl, (e) C5-C6 cycloalkenyl, (f) halo C1-C6 alkyl and (g) halo C2-C6 alkenyl ; wherein Y'is-CH2-,-O-,-S-or-NH-and Y2 is-C (=O)-or-C (Raa) (Rbb)-wherein Raa and Rbb are indepedently selected from the group consisting of hydrogen, Cl-C3 loweralkyl, hydroxymethyl, 1-hydroxyethyl, 2-hydroxyethyl, aminomethyl, 1-aminoethyl, 2-aminoethyl, thiolmethyl, 1-thiolethyl, 2-thiolethyl, methoxymethyl, N-methylaminomethyl and methylthiomethyl ; R3 and R4 are independently selected from the group consisting of (a) hydrogen, (b) cycloalkyl, (c) cycloalkenyl, (d) heterocyclic, (e) aryl and (f)-Z-R14 wherein Z is (i) -C(R37a)(R37b)-, (ii)-C(R47)=C(R48)-, (iii)-C=C-, (iv) -C(=O)-, (v) -C(=S)-, (vi) -C(=NR15)-, (vii) -C(R37a)(OR37c)-, (viii)-C (R37a)(SR37c)-, (ix) -C(R37a)(N(R37b)(R37c))-, (x) -C(R37a)(R37b)-O-, (xi)-C -C(R37a)(R37b)-N(R37c)-, (xii) -C(R37a)(R37b)-N(O)(R37c)-, (xiii)-C(R)(R)-N(OH)-.(xiv)-C(R)(R)-S-.

(xv) -C(R37a)(R37b)-S(O)-, (xvi) -C(R37a)(R37b)-S(O)2-, (xvii) -C(R37a)(R37b)-C(=O)-, (xviii) -C(R37a)(R37B)-C(=S)-, (xix) C (R37a)(R37b)-C(=NR15)-, (xx) -C(R37a)(OR37c)-C(=O)-, (xxi) -C(R37a)(SR37c)-C(=O)-, (xxii) -C(R37a)(OR37c)-C(=S)-, (xxiii) -C(R37a)(SR37c)-C(=S)-, (xxiv) -C(=O)-C(R37a)(OR37c)-, (xxv) -C(=O)-C(R37a)(SR37c)-, (xxvi) -C(=S)-C(R37a)(OR37c)-, (xxvii) -C(=S)-C(R37a)(SR37c)-, (xxviii) -C(R37a)(OR37c)-C(R37a)(OR37c)-, (xxix) -C(R37a)(SR37c)-C(R37a)(OR37c)-, (xxx) -C(R37a)(OR37c)-C(R37a)(SR37c)-, (xxxi) -C(R37a)(SR37c)-C(R37a)(SR37c)-, (xxxii) -C(=O)-C(=O)-, (xxxiii) -C(=S)-C(=S)-, (xxxiv) -C(=O)-O-, (xxxv) -C(=O)-S-, (xxxvi) -C(=S)-O-, (xxxvii) -C(=S)-S-, (xxxviii) -C(=O)-N(R37a)-, (xxxix) -C(=S)-N(R37a)-, (xl) -C(R37a)(R37b)-C(=O)-N(R37a)-, <BR> <BR> <BR> <BR> <BR> (xli) -C(R37a)(R37b)-C(=S)-N(R37a)-, (xlii) -C(R37a)(R37b)-C(=O)-O-,<BR> (xliii) -C(R37a)(R37b)-C(=O)-S-, (sliv) -C(R37a)(R37b)-C(=S)-O-, (xlv) -C(R37a)(R37b)-C(=S)-S-, (xlvi) -C(R37a)(R37b)-N(R37b)-C(=O)-, (xlvii) -C(R37a)(R37b)-N(R37b)-C(=S)-, (xlviii) -C(R37a)(R37b)-O-C(=O)-, (xlix) -C(R37a)(R37b)-S-C(=O)-, (l) -C(R37a)(R37b)-O-C(=S)-, (li)-C(R37a)(R37b)-S-C(=S)-, (lii) -C(R37a)(R37b)-N(R37b)-C(=O)-N(R37a)-, (liii) -C(R37a)(R37b)-N(R37b)-C(=S)-N(R37a)-, <BR> <BR> <BR> <BR> <BR> <BR> (liv) -C(R37a)(R37b)-N(R37b)-C(=O)-O-,<BR> (lv) -C(R37a)(R37b)-N(R37b)-C(=O)-S-, (lvi) -C(R37a)(R37b)-N(R37b)-C(=S)-O-, (lvii) -C(R37a)(R37b)-N(R37b)-C(=S)-S-, (lviii) -C(R37a)(R37b)-O-C(=O)-N(R37a)-, (lix) -C(R37a)(R37b)-S-C(=O)-N(R37a)-, (lx) -C(R37a)(R37b)-O-C(=S)-N(R37a)-, (lxi) -C(R37a)(R37b)-S-C(=S)-N(R37a)-, (lxii) -C(R37a)(R37b)-O-C(=O)-O-, (txiii)-C(R)(R)-S-C(=0)-0-,()xiv)-C(R)(R)-0-C(=0)-S-.

(lxv) -C(R37a)(R37b)-S-C(=O)-S-, (lxvi) -C(R37a)(R37b)-O-C(=S)-O-, (lxvii) -C(R37a)(R37b)-S-C(=S)-O-, (lxviii) -C(R37a)(R37b)-O-C(=S)-S-, (lxix) -C(R37a)(R37b)-S-C(=S)-S- or (lxx) -C(R37a)(R37b)-C(R37a)(OR37c)-; R14 is (i) hydrogen, (ii) Ci-Ci2 alkyl, (iii) haloalkyl, (iv) hydroxyalkyl, (v) thiol-substituted alkyl, (vi) R37CO-substituted alkyl, (vii) R37CS-substituted alkyl, (viii) aminoalkyl, (ix) (R37C) NH-substituted alkyl, (x) (R37a) (R37C) N-susbstituted alkyl (xi) R37aO-(O=) C-substituted alkyl, (xii) R37aS-(O=) C-substituted alkyl, (xiii) R37aO-(S=) C-substituted alkyl, (xiv) R37aS-(S=) C-substituted alkyl, (xv) (R37aO)2-P(=O)-substituted alkyl, (xvi) cyanoalkyl, (xvi) C2-C12 alkenyl, (xviii) haloalkenyl, (xix) C2-C2 alkynyl, (xx) cycloalkyl, (xxi) (cycloalkyl) alkyl, (xxii) (cycloalkyl) alkenyl, (xxiii) (cycloalkyl) alkynyl, (xxiv) cycloalkenyl, (xxv) (cycloalkenyl) alkyl, (xxvi) (cycloalkenyl) alkenyl, (xxvii) (cycloalkenyl) alkynyl, (xxviii)aryl, (xxix) (aryl) alkyl, (xxx) (aryl) alkenyl, (xxxi) (aryl) alkynyl, (xxxii) heterocyclic, (xxxiii) (heterocyclic) alkyl, (xxxiv) (heterocyclic) alkenyl or (xxxv) (heterocyclic) alkynyl, with the proviso that R'4 is other than hydrogen when Z is -C(R37a)(R37b)-N(R37b)-C(=O)-O-, -C(R37a)(R37b)-N(R37b)-C(=S)-O-, -C(R37a)(R37b)-N(R37b)-C(=O)-S-, -C(R37a)(R37b)-N(R37b)-C(=S)-S-, -C(R37a)(R37b)-O-C(=O)-O-, -C(R37a)(R37b)-O-C(=S)-O-, -C(R37a)(R37b)-S-C(=O)-O-, -C(R37a)(R37b)-S-C(=S)-O-, -C(R37a)(R37b)-O-C(=O)-S-, -C(R37a)(R37b)-O-C(=S)-S-, -C(R37a)(R37b)-S-C(=O)-S- or -C(R37a)(R37b)-S-C(=S)-S-; p37ap37bp47gp48g occurrence are independently selected from the group consisting of (i) hydrogen, (ii) C,-C, 2 alkyl, (iii) haloalkyl, (iv) hydroxyalkyl, (v) alkoxyalkyl, (vi) C2-C, 2 alkenyl, (vii) haloalkenyl, (viii) C2-Ci2 alkynyl, (ix) cycloalkyl, (x) (cycloalkyl) alkyl, (xi) (cycloalkyl) alkenyl, (xii) (cycloalkyl) alkynyl, (xiii) cycloalkenyl, (xiv) (cycloalkenyl) alkyl, (xv) (cycloalkenyl)- alkenyl, (xvi) (cycloalkenyl) alkynyl, (xvii) aryl, (xviii) (aryl) alkyl, (xix) (aryl) alkenyl, (xx) (aryl) alkynyl, (xxi) heterocyclic, (xxii) (heterocyclic) alkyl, (xxiii) (heterocyclic) alkenyl and (xxiv) (heterocyclic) alkynyl ; R37c at each occurrence is independently selected from the group consisting of (i) hydrogen, (ii) Ci-Ci2 alkyl, (iii) haloalkyl, (iv) C2-C, 2 alkenyl, (v) haloalkenyl, (vi) C2-C, 2 alkynyl, (vii) cycloalkyl, (viii) (cycloalkyl) alkyl, (ix) (cycloalkyl) alkenyl, (x) (cycloalkyl) alkynyl, (xi) cycloalkenyl, (xii) (cycloalkenyl) alkyl, (xiii) (cycloalkenyl) alkenyl, (xiv) (cycloalkenyl) alkynyl, (xv) aryl, (xvi) (aryl) alkyl, (xvii) (aryl) alkenyl, (xviii) (aryl) alkynyl, (xix) heterocyclic, (xx) (heterocyclic) alkyl, (xxi) (heterocyclic) alkenyl, (xxii) (heterocyclic) alkynyl, (xxiii)-C (=O)-R'4, (xxiv)-C (=S)-R'4, (xxv)-S (O) 2-R'4 and (xxvi) hydroxyalkyl ; or when Z is-C (R37a)(R37b)-N(R37c)-, then N (R37C) and Rl4 when taken together are an azido group; or when Z is -C(R37a)(R37b)-N(O)(R37c)-, then N (O) (R37C) and R when taken together are an N-oxidized 3-7 membered heterocyclic ring having at least one N-oxidized ring nitrogen atom ; or when Z is-C (R37a)(R37b)-, -C(R37a)(OR37c)-, -C(R37a)(SR37c)- or -C (R37a) (N (R37b)(R37c))-, then R37a, R14 and the carbon atom to which they are bonded when taken together form a cyclopentyl, cyclopentenyl, cyclohexyl or cyclohexenyl ring or then OR 37c or SR37C or N (R37c) and R14 and the carbon atom to which they are bonded when taken together form a heterocyclic ring containing an O, S or N atom, respectively, and having from 4 to 8 ring atoms; R'5 is selected from the group consisting of (i) hydrogen, (ii) hydroxy, (iii) amino, (iv) Cl-Cl2 alkyl, (v) haloalkyl, (vi) C2-Ci2 alkenyl, (vii) haloalkenyl, (viii) cycloalkyl, (ix) (cycloalkyl) alkyl, (x) (cycloalkyl) alkenyl, (xi) cycloalkenyl, (xii) (cycloalkenyl) alkyl, (xiii) (cycloalkenyl) alkenyl, (xiv) aryl, (xv) (aryl) alkyl, (xvi) (aryl) alkenyl, (xvii) heterocyclic, (xviii) (heterocyclic) alkyl and (xix) (heterocyclic) alkenyl ; or R3 and R4 taken together, with the atom to which they are attached, form a carbocyclic or heterocyclic ring having from 3 to 8 ring atoms; R5 is selected from the group consisting of (a) hydrogen, (b)-CH (R3a) 2, (c)-0-R4°, (d) C2-C4 alkynyl, (e) cyclopropyl, (f) cyclobutyl, (g)-C (=Q1)-R17, and (h) -N(R19)2 wherein Q'is O, S, or N (R18) ; R17 and R18 are independently selected, at each occurrence, from the group consisting of hydrogen, methyl, and ethyl ; R19, R38, and R40 are independently selected, at each occurrence, from the group consisting of (i) hydrogen, (ii) Ci-Ci2 alkyl, (iii) haloalkyl, (iv) C2-C12 alkenyl, (v) haloalkenyl, (vi) cycloalkyl, (vii) (cycloalkyl) alkyl, (viii) (cycloalkyl) alkenyl, (ix) cycloalkenyl, (x) (cycloalkenyl) alkyl, (xi) (cycloalkenyl) alkenyl, (xii) aryl, (xiii) (aryl) alkyl, (xiv) (aryl) alkenyl, (xv) heterocyclic, (xvi) (heterocyclic) alkyl and (xvii) (heterocyclic) alkenyl ; Y is selected from the group consisting of (a) hydrogen, (b) Cr-C5 alkyl, (c) Cr-C5 haloalkyl, (d) C2-C5 alkenyl, (e) C2-C5 haloalkenyl, (f) C2-C5 alkynyl, (g) C3-C5 cycloalkyl, (h) C3-C5 cycloalkyl-C,-to-C3-alkyl, (i) C5 cycloalkenyl, (j) C5 cycloalkenyl-C,-to- C3-alkyl, (k) C5 cycloalkenyl-C2-to-C3-alkenyl, (I)-(CHR39) nOR20, (m)-CH (OR20)- CH2 (OR20), (n) -(CHR39)nSR21, (o) -(CHR39)nCN, (p) -(CHR39)nN3, (q) phenyl, (r) halo-substituted phenyl, (s)-(CHR39) nC (=Q2) R22, (t)-(CHR39) nN (=Q3), (u)-N (O) =CHCH3, (v) -(CHR39)nNR23R24, (w) halo and (x) a heterocyclic ring having from 3 to 6 ring atoms; wherein n is 0, 1, or 2; Q2 is O, S, NR25, or CHR26 ; and Q3 is NR41, or CHR 42 ; R20 at each occurrence is independently (i) hydrogen, (ii) methyl, (iii) ethyl, (iv) n-propyl, (v) isopropyl, (vi) C1-C3 haloalkyl, (vii) vinyl, (viii) propenyl, (ix) isopropenyl, (x) allyl, (xi) C2-C3 haloalkenyl, (xii) amino, (xiii)-NHCH3, (xiv)-N (CH3) 2, (xv)-NHCH2CH3, (xvi)-N (CH3) (CH2CH3), (xvii)-N (CH2CH3) 2 or (xviii)-N (=CH2); R21 is hydrogen, (ii) methyl, (iii) ethyl, (iv) n-propyl, (v) isopropyl, (vi) Cl-C3 haloalkyl, (vii) vinyl, (viii) propenyl, (ix) isopropenyl, (x) allyl or (xi) C2-C3 haloalkenyl ; R22 is (i) hydrogen, (ii) methyl, (iii) ethyl, (iv) n-propyl, (v) isopropyl, (vi) hydroxy, (vii) thiol, (viii) methoxy, (ix) ethoxy, (x) n-propoxy, (xi) isopropoxy, (xii) cyclopropyloxy, (xiii) methylthio, (xiv) ethylthio, (xv) n-propylthio, (xvi) isopropylthio, (xvii) cyclopropylthio, (xviii) vinyl, (xix) propenyl, (xx) isopropenyl, (xxi) allyl, (xxii)-N (R25a) (R28b), (xxiii) -CH2R29, (xxiv) aminomethyl, (xxv) hydroxymethyl, (xxvi) thiolmethyl, (xxvii)-NHNH2, (xxviii)-N (CH3) NH2 or (xxix)-NHNH (CH3); R23 and R39 are independently hydrogen or methyl ; R41 and R42 are independently hydrogen, methyl, or ethyl ; R24 is selected from the group consisting of (i) hydrogen, (ii) Ci-C4 alkyl, (iii) C2-C4 alkenyl, (iv) C2-C4 alkynyl, (v) cyclopropyl, (vi)-C (=Q4)-R3°, (v)-OR3', and (vi)-N (R32) 2, wherein Q4 is O, S, or N (R33) ; R25 is hydrogen, hydroxy, methyl, ethyl, amino,-CN, or-N02 ; R26 group is hydrogen, methyl or ethyl ; R28a hydrogen, hydroxy, methyl, ethyl, amino,-NHCH3,-N (CH3) 2, methoxy, ethoxy, or-CN; R28b is hydrogen, methyl or ethyl ; or R28a, R28b and the nitrogen to which they are bonded taken together represent azetidinyl ; R29 group is hydrogen, hydroxy, thiol, methyl, ethyl, amino, methoxy, ethoxy, methylthio, ethylthio, methylamino or ethylamino ; R30 group is hydrogen, methyl, ethyl,-oR34-SR34,-N (R35) 2,-NHOH, -NHNH2,-N (CH3) NH2, or-N (CH2CH3) NH2; R31 and R32 substituents, at each occurrence, are independently hydrogen, methyl or ethyl ; R33 group is hydrogen, hydroxy, methyl, ethyl, amino,-CN, or-N02 ; R34 group is methyl or ethyl ; R35 group is independently hydrogen, methyl or ethyl ; with the proviso that when Q2is CHR26 then R22 is selected from the group consisting of hydrogen,-CH3,-C2H5,-C3H7,-OCH3,-SCH3,-O-C2H5, and -S-C2H5, and with the proviso that when R3 and R4 are each hydrogen, then Y is other than hydrogen; R6 and R7 are independently selected from the group consisting of (a) hydrogen, (b) C1-C12 alkyl, (c) C2-C12 alkenyl, (d) cycloalkyl, (e) (cycloalkyl)alkyl, (f) (cycloalkyl)alkenyl, (g) cycloalkenyl, (h) (cycloalkenyl) alkyl, (i) (cycloalkenyl) alkenyl, (j) aryl, (k) (aryl) alkyl, (I) (aryl) alkenyl, (m) heterocyclic, (n) (heterocyclic) alkyl and (o) (heterocyclic) alkenyl ; and R8, R9, and R° are independently selected from the group consisting of (a) hydrogen, (b) C,-C6 alkyl, (c) C2-C6 alkenyl, (d) C3-C6 cycloalkyl, (e) C3-C6 cycloalkenyl, and (f) fluorine, with the proviso that the total number of atoms, other than hydrogen, in each of R8, R9, and R10, is 6 atoms or less.

Preferred compounds of the invention are compounds having the relative sterochemistry depicted by Formula IIA : or a pharmaceutically acceptable salt, ester or prodrug thereof, wherein R', R2, R3, R4, R5, R6, R7, R8, R9, R°, X and Y are as defined above and wherein R3 and R4 are not both the same.

More preferred compounds of the invention are enantiomerically enriched compounds having the absolute sterochemistry depicted by Formula IIB : IIB or a pharmaceutically acceptable salt, ester or prodrug thereof, wherein R1, R2, R3, R4, R5, R6, R7, R8, R9, R°, X and Y are as defined above and wherein R3 and R4 are not both the same.

Other preferred compounds of the invention are compounds having the relative sterochemistry depicted by Formula IIIA : or a pharmaceutically acceptable salt, ester or prodrug thereof, wherein R', R2, R3, R4, R5, R6, R7, R8, R9, R10, X and Y are as defined above and wherein R3 and R4 are not both the same.

Other more preferred compounds of the invention are enantiomerically enriched compounds having the absolute sterochemistry depicted by Formula IIIB or a pharmaceutically acceptable salt, ester or prodrug thereof, wherein R', R2, R3, R4, R5, R6, R7, R8, R9, R10, X and Y are as defined above and wherein R3 and R4 are not both the same.

Other preferred compounds of the invention are compounds having Formula I, IIA, IIB, IIIA or IIIB or a salt, ester or prodrug thereof wherein R1 is defined as above ; -X-R is R2-C (=O)-NH-, R2-NH-C (=O)-, R2-NH-S02-or R2-SO2-NH- wherein R is Cr-C3 loweralkyl, halo C1-C3 loweralkyl, C2-C3 alkenyl or halo C2-C3 alkenyl or-X-R2 is wherein Y'is-CH2-,-O-,-S-or-NH-and y2 is-C (=O)-or-C (Raa) (Rbb)-wherein Raa and Rbb are independently selected from the group consisting of hydrogen, Cl-C3 loweralkyl, hydroxymethyl, 1-hydroxyethyl, 2-hydroxyethyl, aminomethyl, 1-aminoethyl, 2-aminoethyl, thiolmethyl, 1-thiolethyl, 2-thiolethyl, methoxymethyl, N-methylaminomethyl and methylthiomethyl ; R3 and R4 are independently selected from hydrogen, heterocyclic and -Z-R'4 wherein Z and R'4 are defined as above and wherein one of R3 and R4 is other than hydrogen; R5 is hydrogen or loweralkyl ; R6 and R'are independently hydrogen or loweralkyl ; R8 and R9 are independently hydrogen, fluoro or loweralkyl ; R10 is hydrogen, fluoro or loweralkyl ; and Y is C2-C5 alkenyl, C2-C5 haloalkenyl,-C (=Q2) R22, -N (=Q3),-N (O) =CHCH3,-NR23R24 or a heterocyclic ring having from 3 to 6 ring atoms, wherein R22, R23, R24, Q2 and Q3 are defined as above.

More preferred compounds of the invention are compounds having Formula I, IIA, IIB, IIIA or IIIB or a salt, ester or prodrug thereof wherein R1 is defined as above; -X-R2 is R2-C(=O)-NH-, R2-NH-C(=O)-, R2-NH-SO2- or R2-SO2-NH- wherein R is Cl-C3 loweralkyl, halo C1-C3 loweralkyl, C2-C3 alkenyl or halo C2-C3 alkenyl or-X-R2 is wherein Y1 is -CH2- and Y2 is-C (=O)- or -C(Raa)(Rbb)- wherein Raa and Rbb are independently selected from the group consisting of hydrogen, Cr-C3 loweralkyl, hydroxymethyl, 1-hydroxyethyl and 2-hydroxyethyl ; R3 and R4 are independently selected from hydrogen, heterocyclic and -Z-R'4 wherein Z and R'4 are defined as above and wherein one of R3 and R4 is other than hydrogen; R5 is hydrogen or loweralkyl ; R6 and R 7are independently hydrogen or loweralkyl ; R and R9 are independently hydrogen or loweralkyl ; R'° is hydrogen or loweralkyl ; and Y is C2-C5 alkenyl, C2@C5 haloalkenyl, -C(=Q2)R22, -N (=Q3),-N (O) =CHCH3,-NR23R24 or a heterocyclic ring having 5 ring atoms and also containing one or two double bonds, wherein R22, R23, R24, Q2 and Q3 are defined as above.

Even more preferred compounds of the invention are compounds having Formula l, IIA, IIB, IIIA or IIIB or a salt, ester or prodrug thereof wherein R1 is defined as above; -X-R2 is R2-C (=O)-NH-, R2-NH-C (=O)-, R2-NH-SO2- or RS-SO2-NH- wherein R2 is Cl-C3 loweralkyl, halo C1-C3 loweralkyl, C2-C3 alkenyl or halo C1-C3 alkenyl or-X-R2 is wherein Y'is-CH2-and Y2 is-C (=O)- or -C(Raa)(Rbb)- wherein Raa and Rbb are independently selected from the group consisting of hydrogen, C,-C3 loweralkyl, hydroxymethyl, 1-hydroxyethyl and 2-hydroxyethyl ; R3 and R4 are independently selected from hydrogen, heterocyclic and -Z-R14 wherein Z and R'4 are defined as above and wherein one of R3 and R4 is other than hydrogen; R5 is hydrogen or loweralkyl ; R6 and R7 are independently hydrogen or loweralkyl ; R8 and R9 are independently hydrogen or loweralkyl ; R'° is hydrogen or loweralkyl ; and Y is C2-C5 alkenyl, C2-C5 haloalkenyl, NH2,-NHC (=NH) NH2 or a heterocyclic ring having 5 ring atoms and also containing one or two double bonds.

More highly preferred compounds of the invention are compounds having Formula I, IIA, IIB, IIIA or IIIB or a salt, ester or prodrug thereof wherein R'is -C02H ; -X-R2 is R2-C(=O)-NH-, R2-NH-C(=O)-, R2-NH-SO2- or R2-SO2-NH- wherein R2 is Ci-C3) loweralkyl or halo-C1-C3 loweralkyl ; R3 and R4 are independently selected from hydrogen, heterocyclic and -Z-R14 wherein Z and R14 are defined as above and wherein one of R3 and R 4 is other than hydrogen; R5 is hydrogen or loweralkyl; R6 and R7 are hydrogen independently hydrogen or loweralkyl ; R8 and R9 are hydrogen independently hydrogen or loweralkyl ; Rlo is hydrogen or loweralkyl ; and Y is C2-C5 alkenyl, C2-C5 haloalkenyl, NH2,-NHC (=NH) NH2 or a heterocyclic ring having 5 ring atoms and also containing one or two double bonds.

Even more highly preferred compounds of the invention are compounds having Formula 1, IIA, IIB, IIIA or IIIB or a salt, ester or prodrug thereof wherein R'is-C02H ; -X-R2 is R2-C (=O)-NH-, R2-NH-C (=O)-, RZ-NH-S02-or R2-SO2-NH-wherein R2 is Ci-C3) loweralkyl or halo- C1-C3 loweralkyl ; R4 is hydrogen or loweralkyl and R3is heterocyclic or-Z-R14 wherein Z and R14 are defined as above; R5 is hydrogen ; R6 and R7 are hydrogen; R8 and R9 are hydrogen; Rlo is hydrogen; and Y is C2-C5 alkenyl, C2-C5 haloalkenyl, NH2,-NHC (=NH) NH2 or a heterocyclic ring having 5 ring atoms and also containing one or two double bonds.

Other even more highly preferred compounds of the invention are compounds having Formula I, IIA, IIB, IIIA or IIIB or a salt, ester or prodrug thereof wherein R'is-C02H ; -X-R2 is R2-C (=O)-NH-, R2-NH-C (=O)-, R2-NH-S02-or R2-SO2-NH- wherein R2 is Cl-C3 loweralkyl or halo C-C3 loweralkyl ; R4 is hydrogen or loweralkyl and R3 is (a) heterocyclic, (b) alkyl, (b) cycloalkyl, (d) cycloalkylalkyl, (e) alkenyl, (f) alkynyl, (g)-C (=O)-R14, (h) -C(R37a)(OR37c)-R14 or (i) -C(R37a)(R37b)-N(O)(R37c)R14 wherein R14 is (i) alkyl, (ii) cycloalkyl, (iii) cycloalkylalkyl, (iv) alkenyl, (v) haloalkyl, (vi) haloalkenyl, (vii) aryl, (viii) arylalkyl, (ix) heterocyclic, (x) (heterocyclic) alkyl, (xi) hydroxyalkyl, (xii) alkoxyalkyl, (xiii) cyanoalkyl, (xiv) (R37aO)-(O=) C-substituted alkyl or (xv) (R37aO) 2-P (=O)-substituted alkyl ; R37a and R37b are independently selected from the group consisting of (i) hydrogen, (ii) loweralkyl and (iii) loweralkenyl ; and R is hydrogen, (ii) loweralkyl or (iii) loweralkenyl ; R is hydrogen; R and R7 are hydrogen; R8 and R9 are hydrogen ; R'° is hydrogen; and Y is C2-C5 alkenyl, C2_C5 haloalkenyl, NH2,-NHC (=NH) NH2 or a heterocyclic ring having 5 ring atoms and also containing one or two double bonds.

Most highly preferred compounds of the invention are compounds having Formula I, IIA, IIB, IIIA or IIIB or a salt, ester or prodrug thereof wherein R1 is -C02H ; -X-R2 is R2-C (=O)-NH-, R2-NH-C (=O)-, R2-NH-S02-or R2-SO2-NH- wherein R2 is Cl-C3 loweralkyl or halo Cr-C3 loweralkyl ; R4 is hydrogen and R3 is (a) heterocyclic, (b) alkyl or (c)-C (R37a)(OR37c)- R'4 wherein R'4 is (i) alkyl, (ii) cycloalkyl, (iii) cycloalkylalkyl, (iv) alkenyl, (v) haloalkyl, (vi) haloalkenyl, (vii) aryl, (viii) arylalkyl, (ix) heterocyclic, (x) (heterocyclic) alkyl, (xi) hydroxyalkyl, (xii) alkoxyalkyl, (xiii) cyanoalkyl, (xiv) (R37aO)-(O=) C-substituted alkyl or (xv) (R37aO) 2-P (=O)-substituted alkyl ; R37a and R37b are independently selected from the group consisting of (i) hydrogen, (ii) loweralkyl and (iii) loweralkenyl ; and R is hydrogen, (ii) Ci-C3 loweralkyl or (iii) allyl; R5 is hydrogen; R and R7 are hydrogen; R8 and R9 are ; R'° is hydrogen; and Y is C2-C5 alkenyl, C2_C5 haloalkenyl, NH2, -NHC (=NH) NH2 or a heterocyclic ring having 5 ring atoms and also containing one or two double bonds.

Other most highly preferred compounds of the invention are compounds having Formula I, IIA, IIB, IIIA or IIIB or a salt, ester or prodrug thereof wherein R'is-C02H ; -X-R2 is R2-C (=O)-NH- or R2-SO2-NH- wherein R2 is Cl-C3 loweralkyl or halo Cl- C3 loweralkyl ; R4 is hydrogen and R3 is (a) heterocyclic, (b) alkyl or (c)-C (R37a)(OR37c)- R'4 wherein R'4 is (i) loweralkyl, (ii) loweralkenyl, (iii) hydroxy-substituted loweralkyl or (iv) alkoxy-substituted loweralkyl ; R37a is (i) hydrogen, (ii) loweralkyl or (iii) loweralkenyl ; and R37c is (i) hydrogen, (ii) C1-C3 loweralkyl or (iii) allyl ; R5 is hydrogen; R and R 7are hydrogen ; R8 and R9 are hydrogen ; R'° is hydrogen ; and Y is C2-C5 alkenyl, C2 C5 haloalkenyl, NH2,-NHC (=NH) NH2 or a heterocyclic ring having 5 ring atoms and also containing one or two double bonds.

Other most highly preferred compounds of the invention are compounds having Formula I, IIA, IIB, IIIA or IIIB or a salt, ester or prodrug thereof wherein R'is-C02H ; -X-R2 is R2-C (=O)-NH- or R2-SO2-NH- wherein R2 is Cl-C3 loweralkyl or halo Cl- C3 loweralkyl ; R4 is hydrogen and R3 is -C(R37a)(OR37c)-R14 wherein R14 is loweralkyl, loweralkenyl or alkoxy-substituted loweralkyl ; R37a is loweralkyl or loweralkenyl ; and R37C is hydrogen, Cl-C3 loweralkyl or allyl ; R5 is hydrogen; R6 and R7 are hydrogen ; R8 and R9 are hydrogen; R'° is hydrogen; and Y is C2-C5 alkenyl, C2 C5 haloalkenyl, NH2,-NHC (=NH) NH2 or a heterocyclic ring having 5 ring atoms and also containing one or two double bonds.

Other most highly preferred compounds of the invention are compounds having Formula I, IIA, IIB, IIIA or IIIB or a salt, ester or prodrug thereof wherein R'is-C02H ; -X-R2 is R2-C (=O)-NH-or R2-SO2-NH-wherein R2 is C1-C3 loweralkyl or halo Cl- C3 loweralkyl ; R4 is hydrogen and R3 is -C(R37a)(OR37c)-R14 wherein R14 is loweralkyl, loweralkenyl or alkoxy-substituted loweralkyl ; R37a is loweralkyl or loweralkenyl ; and R is hydrogen, Cl-C3 loweralkyl or allyl ; R5 is hydrogen; R6 and R7 are hydrogen; R8 and R9 are hydrogen; R'° is hydrogen; and Y is C2-C5 alkenyl.

Preferred substituents R'include-C02H or esters or prodrugs thereof.

Preferred esters include Ci-Ce loweralkyl esters, cycloalkyl esters (for example, cyclopropyl ester, cyclohexyl ester and the like), cycloalkylalkyl esters, aryl esters (for example, phenyl ester, 2-methylphenyl ester and the like), arylalkyl esters (for example, benzyl ester, phenylethyl ester and the like), haloalkyl esters (for example, 2,2,2-trichloroethyl ester and the like), heterocyclic esters (for example, N-methylpiperazin-4-yl ester and the like), (heterocyclic) alkyl esters (for example, pyridyl methyl ester, pyridylethyl ester, N-methylpiperazin-4-ylmethyl ester, piperidin-1-ylmethyl ester, morpholin-4-ylmethyl ester, 2- (piperidin-1-yl) ethyl ester, 2-(morpholin-4-yl)ethyl ester, 2 (-N-methylpiperazin-4-yl) ethyl ester, 1, 1- dimethyl-2- (piperidin-1-yl) ethyl ester, 1,1-dimethyl-2- (morpholin-4-yl) ethyl ester, 1, 1-dimethyl-2-(N-methylpiperazin-4-yl)ethyl ester, phthalidylmethyl ester and the like), di-loweralkylaminoalkyl esters (for example, 2-N, N-dimethylaminoethyl. ester, 2-N, N-diethylaminoethyl ester and the like), acyloxyalkyl esters (for example, t-butylcarbonyloxymethyl ester and the like), alkoxycarbonyloxyalkyl esters (for example, t-butyloxycarbonyloxymethyl ester and the like), di- loweralkylaminocarbonylalkyl esters (for example, N, N-dimethylamino- carbonylmethyl ester, N, N-diethylaminocarbonylmethyl ester and the like), acylalkyl esters (for example, t-butylcarbonylmethyl ester and the like), (heterocyclic) carbonylalkyl esters (for ester, morpholin-4-ylcarbonylmethyl ester, N-methylpiperazin-4-ylcarbonylmethyl ester and the like), di-loweralkylaminocarbonyloxyalkyl esters (for example, N, N- dimthylaminocarbonyloxymethyl ester, N, N-diethylaminocarbonyloxymethyl ester, N-t-butyl-N-methyl-aminocarbonyloxymethyl ester and the like), alkoxycarbonylalkyl esters (for example, ethoxycarbonylmethyl ester, isopropoxycarbonylmethyl ester and the like), (heterocyclic) carbonyloxyalkyl esters (for example, pyridylcarbonyloxymethyl ester and the like) and the like.

Preferred substituents R1 also include -S(O)2NHC(=O)R11 wherein R11 is defined as above.

Most highly preferred substituents R'include-C02H or esters or prodrugs thereof. Most highty preferred esters inctude Ci-Ce) owera ! ky) esters, cycloalkyl esters, cycloalkylalkyl esters or substituted or unsubstituted benzyl esters.

Preferred substituents-X-R2 include R2-C (=O)-NH-, R2-NH-C (=O)-, R2-NH-SO2- or R2-SO2-NH- wherein R2 is C1-C3 loweralkyl, halo C1-C3 loweralkyl, C2-C3 alkenyl or halo C2-C3 alkenyl or-X-R2 is wherein Y1 is -CH2-, -O-, -S- or -NH- and Y2 is -C(=O)- or -C(Raa)(Rbb)- wherein Raa and Rbb are independently selected from the group consisting of hydrogen, Cl-C3 loweralkyl, hydroxymethyl, 1-hydroxyethyl, 2-hydroxyethyl, aminomethyl, 1-aminoethyl, 2-aminoethyl, thiolmethyl, 1-thiolethyl, 2-thiolethyl, methoxymethyl, N-methylaminomethyl and methylthiomethyl.

More preferred substituents-X-R2 include R2-C (=O)-NH-, R2-NH-C(=O)-, R2-NH-SO2- or R2-SO2-NH- wherein R2 is C1-C3 loweralkyl, halo C1-C3 loweralkyl, C2-C3 alkenyl or halo C2-C3 alkenyl or-X-R2 is wherein Y1 is -CH2- and Y2 is -C(=O)- or -C(Raa)(Rbb)- wherein Raa and Rbb are independently selected from the group consisting of hydrogen, Cl-C3 loweralkyl, hydroxymethyl, 1-hydroxyethyl and 2-hydroxyethyl.

Even more preferred substituents-X-R2 include R2-C (=O)-NH-, R2-NH-C (=O)-, R2-NH-S02-or R2-SO2-NH-wherein RZ is Cl-C3 loweralkyl, halo Cr-C3 loweralkyl, C2-C3 alkenyl or halo C2-C3 alkenyl.

More highly preferred substituents-X-R2 include R2-C (=O)-NH-, R2-NH-C(=O)-, R2-NH-SO2- or R2-SO2-NH- wherein R2 is Cl-C3 loweralkyl or halo-Ci-C3 loweralkyl.

Even more highly preferred substituents-X-R2 include R2-C (=O)-NH-, R2-NH-C(=O)-, R2-NH-SO2- or R2-SO2-NH- wherein R2 is Cl-C2 loweralkyl or halo Ci-C2) loweralkyl, and especially, CH3-C (=O)-NH-, CF3-C (=O)-NH-, CH3-SO2-NH- or CF3-SO2-NH-.

Preferred substituents R3 and R4 are independently selected from the group consisting of hydrogen, heterocyclic and-Z-R'4 wherein Z and R14 are defined as most broadly defined previously herein and wherein one of R3 and R4 is other than hydrogen.

More highly preferred, substituent R4 is hydrogen or loweralkyl and R3 includes heterocyclic or -Z-R14 wherein Z and R'4 are defined as most broadly defined previously herein.

Even more highly preferred, substituent R4 is hydrogen or loweralkyl and R3 includes (a) heterocyclic, (b) alkyl, (c) cycloalkyl, (d) cycloalkylalkyl, (e) alkenyl, (f) alkynyl, (g) -C(=O)-R14, (h) -C(R37a)(OR37c)-R14 or (i) -C(R37a)(R37b)-N(O)(R37c)R14 wherein R'4 is (i) alkyl, (ii) cycloalkyl, (iii) cycloalkylalkyl, (iv) alkenyl, (v) haloalkyl, (vi) haloalkenyl, (vii) aryl, (viii) arylalkyl, (ix) heterocyclic, (x) (heterocyclic) alkyl, (xi) hydroxyalkyl,- (xii) alkoxyalkyl, (xiii) cyanoalkyl, (xiv) (R37aO)-(O=) C-substituted alkyl or (xv) (R37aO) 2-P (=O)-substituted alkyl; R37a and R37b are independently selected from the group consisting of (i) hydrogen, (ii) loweralkyl and (iii) loweralkenyl ; and R37c is (i) hydrogen, (ii) loweralkyl or (iii) loweralkenyl.

Most highly preferred, substituent R4 is hydrogen and R3 inclues (a) heterocyclic, (b) alkyl or (c)-C (R37a)(OR37c)-R14 wherein R'4 is (i) alkyl, (ii) cycloalkyl, (iii) cycloalkylalkyl, (iv) alkenyl, (v) haloalkyl, (vi) haloalkenyl, (vii) aryl, (viii) arylalkyl, (ix) heterocyclic, (x) (heterocyclic) alkyl, (xi) hydroxyalkyl, (xii) alkoxyalkyl, (xiii) cyanoalkyl, (xiv) (R37aO)-(O=) C-substituted alkyl or (xv) (R37aO) 2-P (=O)-substituted alkyl ; R37a and R37b are independently selected from the group consisting of (i) hydrogen, (ii) loweralkyl and (iii) loweralkenyl ; and R37c is (i) hydrogen, (ii) Cl-C3 loweralkyl or (iii) allyl.

Also most highly preferred, substituent R4 is hydrogen and R3 includes (a) heterocyclic, (b) alkyl or (c)-C (R37a)(OR37c)-R14 wherein R14 is (i) loweralkyl, (ii) loweralkenyl, (iii) hydroxy-substituted loweralkyl or (iv) alkoxy-substituted loweralkyl ; R37a is (i) hydrogen, (ii) loweralkyl or (iii) loweralkenyl ; and R37c is (i) hydrogen, (ii) Cl-C3 loweralkyl or (iii) allyl.

Also most highly preferred, substituent R4 is hydrogen and R3 includes -C (R37a)(OR37c)-R14 wherein R'4 is loweralkyl, loweralkenyl or alkoxy-substituted loweralkyi ; R37a is loweralkyl or loweralkenyl ; and R37c is hydrogen, Ci-C3) loweralkyl or allyl, and especially, wherein R37C is hydrogen or methyl.

Preferred substituents R5 include hydrogen or loweralkyl. Most highly preferred, R5 is hydrogen.

Preferred substituents R6 and R7 include independently hydrogen and loweralkyl. Most highly preferred, R6 and R7 are hydrogen.

Preferred substituents R8, R9 and R ° incude independently hydrogen, fluoro and loweralkyl. Most highly preferred, R8, R9 and R10 are hydrogen.

Preferred substituent Y includes C2-C5 alkenyl, C2-C5 haloalkenyl, -C (=Q2) R22,-N (=Q3),-N (O) =CHCH3,-NR23R24 or a heterocyclic ring having from 3 to 6 ring atoms, wherein R22, R23, R24, Q2 and Q3 are defined as above.

More preferred substituent Y includes C2-C5 alkenyl, C2-C5 haloalkenyl, -C(=Q2)R22, -N(=Q3), -N(O)=CHCH3, -NR23R24 or a heterocyclic ring having 5 ring atoms and also containing one or two double bonds, wherein R22 R23 R24 Q2and Q3aredefined as above.

Even more preferred substituent Y includes C2-C5 alkenyl, C2-C5 haloalkenyl, NH2,-NHC (=NH) NH2 or a heterocyclic ring having 5 ring atoms and also containing one or two double bonds. Representative alkenyl and haloalkenyl substituents Y include : -CH=CH2,-CH=CHF,-CH=CH-CH3,-CH=CH-CF3,--CH=CHCI,-CH=CHBr, -CH=CF2,-CH=CF (CH3),-CH=CF (CF3),-CH=CFCI,-CH=CFBr,-CH=C (CH3) 2, -CH=C (CH3) (CF3),-CH=CCI (CH3),-CH=CBr (CH3),-CH=C (CF3) 2,-CH=CCI (CF3), -CH=CBr (CF3),-CH=CC12,-CH=CCIBr,-CF=CH2,-CF=CHF,-CF=CH-CH3, -CF=CH-CF3,-CF=CHCI,-CF=CHBr,-CF=CF2,-CF=CF (CH3),-CF=CF (CF3), -CF=CFCI,-CF=CFBr,-CF=C (CH3) 2,-CF=C (CH3) (CF3),-CF=CCI (CH3), -CF=CBr (CH3),-CF=C (CF3) 2,-CF=CCI (CF3),-CF=CBr (CF3),-CF=CC12, -CF=CCIBr,-C (CH3) =CH2,-C (CH3) =CHF,-C (CH3) =CH-CH3,-C (CH3) =CH-CF3, -C (CH3) =CHCI,-C (CH3) =CHBr,-C (CH3) =CF2,-C (CH3) =CF (CH3), -C (CH3) =CF (CF3),-C (CH3) =CFCI,-C (CH3) =CFBr,-C (CH3) =C (CH3) 2, -C (CH3) =C (CH3) (CF3),-C (CH3) =CCI (CH3), -C (CH3) =CBr (CH3),-C (CH3) =C (CF3) 2,-C (CH3) =CCI (CF3),-C (CH3) =CBr (CF3), -C (CH3) =CC12,-C (CH3) =CCIBr,-C (CF3) =CH2,-C (CF3) =CHF,-C (CF3) =CH-CH3, -C (CF3) =CH-CF3,-C (CF3) =CHCI,-C (CF3) =CHBr,-C (CF3) =CF2, -C (CF3) =CF (CH3),-C (CF3) =CF (CF3),-C (CF3) =CFCI,-C (CF3) =CFBr, -C (CF3) =C (CH3) 2,-C (CF3) =C (CH3) (CF3),-C (CF3) =CCI (CH3), -C (CF3) =CBr (CH3),-C (CF3) =C (CF3) 2,-C (CF3) =CCI (CF3),-C (CF3) =CBr (CF3), -C (CF3) =CCI2,-C (CF3) =CCIBr,-CCI=CH2,-CCI=CHF,-CCI=CH-CH3,-CCI=CH- CF3,-CCI=CHCI,-CCI=CHBr,-CCI=CF2,-CCI=CF (CH3),-CCI=CF (CF3), -CCI=CFCI,-CCI=CFBr,-CCI=C (CH3) 2, -CCI=C (CH3) (CF3),-CCI=CCI (CH3),-CCI=CBr (CH3),-CCI=C (CF3) 2, <BR> <BR> <BR> <BR> <BR> -CCI=CCI (CF3),-CCI=CBr (CF3),-CCI=CCI2,-CCI=CCIBr,<BR> <BR> <BR> <BR> <BR> <BR> <BR> -CH=CH-CH2CH3,-CH=CF-CH2CH3,-CF=CH-CH2CH3,-CF=CF-CH2CH3, -CH=C (CH3) (CH2CH3),-CF=C (CH3) (CH2CH3),-CH=CCI (CH2CH3), -CF=CCI (CH2CH3),-C (CH3) =CH-CH2CH3,-C (CH3) =CF-CH2CH3, -CCI=CH-CH2CH3,-CCI=CF-CH2CH3,-C (CH2CH3) =CH2,-C (CH2CH3) =CHF, -C (CH2CH3) =CF2,-C (CH2CH3) =CH-CH3,-C (CH2CH3) =CF-CH3, -C (CH2CH3) =CH-CI,-C (CH2CH3) =CFCI.

Representative Y substituents which are heterocyclic rings having 5 ring atoms and also containing one or two double bonds include : furanyl, dihydrofuranyl, didehydrodioxolanyl, dithiolyl, imidazolyl, imidazolinyl, isothiazolyl, isothiazolinyl, isoxazolyl, isoxazolinyl, oxadiazolyl, oxadiazolinyl, oxathiolyl, oxazolyl, oxazolinyl, pyrazolyl, pyrazolinyl, pyrrolyl, dihydropyrrolyl, tetrazolyl, tetrazolinyl, thiadiazolyl, thiadiazolinyl, thiazolyl, thiazolinyl, thienyl, dihydrothienyl, triazolyl, triazolinyl.

More highly preferred substituents Y include cis-propenyl, trans-propenyl, isobutenyl, cis-2-chlorovinyl, vinyl, 2,2-difluorovinyl, imidazolyl, pyrazolyl, oxazolyl, isoxazolyl, thiazolyl, isoxazolyl, NH2,-NHC (=NH) NH2.

Most highly preferred substituents Y include cis-propenyl, cis-2-chlorovinyl, vinyl, imidazolyl, pyrazolyl, oxazolyl, isoxazolyl, thiazolyl, isoxazolyl, NH2, -NHC (=NH) NH2, especially, cis-propenyl.

Preferred definitions for the substituents in the compounds of the invention also apply to the intermediates disclosed herein that are useful in the preparation of the compounds of the invention.

Preferred compounds of the invention include compounds having the indicated relative stereochemistry selected from the group consisting of: (t)-(2R, 3S, 5R, 1'R)-2-(1-Acetamido-2-ethyl-2-hyd roxy) butyl-3-(cis-propen-1-yl)- pyrrolidine-5-carboxylic Acid; (t)- (2R, 3S, 5R, 1'R, 2'S)-2- (1-Acetamido-2-hydroxy-2-methyl) pentyl-3- (cis-propen- 1-yl)-pyrrolidine-5-carboxylic Acid; ()- (2R, 3S, 5R, 1'R, 2'S)-2-(1-Acetamido-2-ethyl-2-hydroxy)pentyl-3-(cis-propen-1 - yl)-pyrrolidine-5-carboxylic Acid; (~)-(2R, 3S, 5R, 1'R, 2'S)-2- (1-Acetamido-2-hydroxy) pentyl-3- (cis-propen-1-yl)- pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt ; ()- (2R, 3R, 5R, 1'R, 2'R)-2- (1-Acetamido-2, 3-dihydroxy) propyl-3- (cis-propen-1-yl)- pyrrolidine-5-carboxylic Acid; ()- (2R, 3S, 5R, 1'R, 2'S)-2- (1-Acetamido-2-hydroxy-4-vinyl) butyl-3- (cis-propen-1- yl)-pyrrolidine-5-carboxylic Acid;.

(-)- (2R, 3S, 5R, 1'S)-2-(1-Acetamido-2-hydroxy)butyl-3-(cis-propen-1-yl)- pyrrolidine-5-carboxylate Ammonium Salt ; ()- (2R, 3S, 5R, 1'R, 2'R)-2- (1-Acetamido-2, 3-dimethoxy) propyl-3- (cis-propen-1-yl)- pyrrolidine-5-carboxylic Acid ; ()- (2R, 3S, 5R, 1'R, 2'S)-2-(1-Acetamido-2-methoxy-2-vinyl)ethyl-3-(cis-propen-1- yl)-pyrrolidine-5-carboxylic Acid; ()- (2R, 3S, 5R, 1'S)-2-(1-Acetamido-2ethyl)butyl-3-(cis-propen-1-yl)-pyrroli dine-5- carboxylic ; ()- (2R, 3S, 5R, 1'S, 3'S)-2-(1-Acetamido-2-(N-isopropyl-N-methylamino-N- oxide)) ethyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic Acid ; (~)-(2R,3S,5R,1'S,3'S)-2-(1-Acetamido-2-(N-ethyl-N-methylami no-N-oxide))ethyl- 3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic Acid ; (~)-(2R, 3S, 5R, 1'R, 2'S)-2- (1-Acetamido-2-methoxy) butyl-3-(cis-propen-1-yl)- pyrrolidine-5-carboxylic Acid; ()- (2R, 3S, 5R, 1'R, 2'S)-2-(1-Acetamido-2-methoxy)pentyl-3-(cis-propen-1-yl)- pyrrolidine-5-carboxylic Acid; ()- (2R, 3R, 5R, 1'R, 2'S)-2-(1-Acetamido-2-hydroxy)butyl-3-(pyrazol-3-yl)- pyrrolidine-5-carboxylic Acid; ()- (2R, 3S, 5R, 1'R, 2'S)-2-(1-Acetamido-2-hydroxy)butyl-3-(cis-propen-1-yl)- pyrrolidine-5-carboxylic Acid; ()- (2R, 3S, 5R, 1'R, 2'R)-2- (1-Acetamido-1- (3, 6-dihydro-2-H-pyran-2-yl)) propyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic Acid; ()- (2R, 3S, 5R, 1'R, 2'S)-2-(1-Acetamido-2-methoxy-2-allyl)ethyl-3-(cis-propen-1- yl)-pyrrolidine-5-carboxylic Acid; ()- (2R, 3S, 5R, 1'R, 2'S, 3'S)-2-(1-Acetamido-2-hydroxy-3-methyl)pentyl-3-(cis- propen-1-yl)-pyrrolidine-5-carboxylic Acid ; (~)-(2R, 3S, 5R, 1'R, 2'S)-2-(1-Acetamido-2-methoxy-4-vinyl)butyl-3-(cis-propen-1- yl)-pyrrolidine-5-carboxylic Acid;.

()- (2R, 3S, 5R, 1'R, 2'S)-2- (1-Acetamido-2-hydroxy-3-cyano) propyl-3- (cis-propen- 1-yl)-pyrrolidine-5-carboxylic Acid; (~)-(2R, 3S, 5R, 1'R, 2'S)-2-(1-Acetamido-1-(3, 6-dihydro-2-H-pyran-2-yl)) methyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic Acid; ()- (2R, 3S, 5R, 1'R, 2'S)-2-(1-Acetamido-2, 3-dimethoxy) propyl-3-(cis-propen-1-yl)- pyrrolidine-5-carboxylic Acid; ()- (2R, 3S, 5R, 1'R, 2'S)-2- (1-Acetamido-2-hydroxymethyl-2-hydroxy) pentyl-3- (cis- propen-1-yl)-pyrrolidine-5-carboxylic Acid ; ()- (2R, 3S, 5R, 1'R, 2'S)-2-(1-Acetamido-2-ethoxy)pentyl-3-(cis-propen-1-yl)- pyrrolidine-5-carboxylic Acid; ()- (2R, 3S, 5R, 1'R, 2'S)-2- (1-Acetamido-2-hydroxy-3-dimethyl) butyl-3- (cis-propen- 1-yl)-pyrrolidine-5-carboxylic Acid; ()- (2R, 3S, 5R, 1'R, 2'S)-2-(1-Acetamido-2-ethoxy-3-vinyl)propyl-3-(cis-propen-1- yl)-pyrrolidine-5-carboxylic Acid; ()- (2R, 3S, 5R, 1'R, 2'S)-2- (1-Acetamido-2-hydroxy-2- (propeny-2-yl)) ethyl-3- (cis- propen-1-yl)-pyrrolidine-5-carboxylic Acid ; ()- (2R, 3S, 5R, 1'R, 2'S)-2- (1-Acetamido-2-hydroxy) hexyl-3- (cis-propen-1-yl)- pyrrolidine-5-carboxylic Acid; ()- (2R, 3S, 5R, 1'S)-2- (1-acetamido-3-methyl) butyl-3- (cis-propen-1-yl)-pyrrolidine- 5-carboxylic Acid; ()- (2R, 3S, 5R, 1'R, 2'S)-2- (1-Acetamido-2-hydroxy) butyl-3-vinyl-pyrrolidine-5- carboxylic Acid; ()- (2R, 3S, 5R, 1'R, 2'S)-2- (1-Acetamido-2-hydroxy) pentyl-3-vinyl-pyrrolidine-5- carboxylic Acid; (~)-(2R, 3S, 5R, 1'R,2'R)-2-(1-Acetamido-2-hydroxyethyl-2-hydroxy)pentyl-3-(c is- propen-1-yl)-pyrrolidine-5-carboxylic Acid ; ()- (2R, 3S, 5R, 1'R, 2'R)-2-(1-Acetamido-2-hydroxy)butyl-3-vinyl-pyrrolidine-5- carboxylic Acid; ()- (2R, 3S, 5R, 1'R, 2'R)-2-(1-Acetamido-2-methoxy)pentyl-3-(cis-propen-1-yl)- pyrrolidine-5-carboxylic ; ()- (2R, 3S, 5R, 1'R, 2'R)-2-(1-Acetamido-2-hydroxy)pentyl-3-vinyl-pyrrolidine-5- carboxylic Acid; ()- (2R, 3S, 5R, 1'R)-2-(1-Acetamido-2-hydroxy) ethyl-3-(cis-propen-1-yl)- pyrrolidine-5-carboxylic Acid; ()- (2R, 3S, 5R, 1'S)-2- (1-acetamido-3-methyl) butyl-3- (cis-2-chloro-vin-1-yl)- pyrrolidine-5-carboxylic Acid; ()- (2R, 3R, 5R, 1'S)-2- (1-acetamido-3-methyl) butyl-3- (pyrazol-3-yl)-pyrrolidine-5- carboxylic Acid; ()- (2R, 3S, 5R, 1'S, 3'R)-2- (1-Acetamido-3-hydroxy) pentyl-3- (cis-propen-1-yl)- pyrrolidine-5-carboxylic Acid ; ()- (2R, 3R, 5R, 1'S)-2- (1-Acetamido-3-methyl) butyl-3- (thiazol-4-yl)-pyrrolidine-5- carboxylic Acid; ()- (2R, 3R, 5R, 1'S)-1-t-Butoxycarbonyl-2-(1-acetamido-3-methyl)butyl-3-(thi azol- 2-yl)-pyrrolidine-5-carboxylic ; ()- (2R, 3S, 5R, 1'S)-2- (1-Acetamido-3-methyl) butyl-3-vinyl-pyrrolidine-5-carboxylic Acid; (t)- (2R, 3S, 5R, 1'S)-2- (1-acetamido-3-methyl) butyl-3- (2, 2-difluoro-vin-1-yl)- pyrrolidine-5-carboxylic Acid; (t)- (2R, 3R, 5R, 1'S)-2- (1-acetamido-3-methyl) butyl-3- (pyrazol-3-yl)-pyrrolidine-5- carboxylic Acid ; ()- (2R, 3R, 5R, 1'S)-2- (1-acetamido-3-methyl) butyl-3- (isoxazol-3-yl)-pyrrolidine-5- carboxylic Acid ; ()- (2R, 3R, 5R, 1'S)-2- (1-acetamido-3-methyl) butyl-3- (isoxazol-5-yl)-pyrrolidine-5- carboxylic ; ()- (2R, 3R, 5R, 1'S)-2- (1-Acetamido-3-methyl) butyl-3- (imidazol-2-yl)-pyrrolidine-5- Carboxylic Acid; ()- (2R, 3R, 5R, 1'S)-2- (1-Acetamido-3-methyl) butyl-3- (imidazol-4-yl)-pyrrolidine-5- carboxylic Acid; ()- (2S, 3R, 5R, 1'S)-2-(1-acetamido-3-methyl)butyl-3-amino-pyrrolidine-5- carboxylic Acid; ()- (2R, 3S, 5R, 1'R, 2'S)-2-(1-Acetamido-2-methyl-2-methoxy)pentyl-3-(cis-propen- 1-yl)-pyrrolidine-5-carboxylic Acid; ()- (2R, 3S, 5R, 1'R, 2'S)-2-(1-Acetamido-2-methyl-2-methoxy)pentyl-3-(cis-propen- 1-yl)-pyrrolidine-5-carboxylic Acid Ethyl Ester; ()- (2R, 3S, 5R, 1'R, 2'S)-2-(1-Acetamido-2-vinyl2-methoxy)pentyl-3-(cis-propen-1- yl)-pyrrolidine-5-carboxylic ; ()- (2R, 3S, 5R, 1'R)-2-(1-Acetamido-2-ethyl-2-methoxy) butyl-3-(cis-propen-1-yl)- pyrrolidine-5-carboxylic ; ()- (2R, 3S, 5R, 1'R, 2'S)-2-(1-Acetamido-2-methyl-2, 3-dimethoxy) propyl-3- (cis- propen-1-yl)-pyrrolidine-5-carboxylic Acid ; and ()- (2R, 3S, 5R, 1'R, 2'S)-2- (1-Acetamido-2-methyl-2-methoxy) butyl-3- (cis-propen- 1-yl)-pyrrolidine-5-carboxylic Acid; or a pharmaceutically acceptable salt, ester or prodrug thereof.

Other preferred compounds of-the-invention include enantiomerically enriched compounds having the indicated absolute stereochemistry selected from the group consisting of: (2R, 3S, 5R, 1'R)-2- (1-Acetamido-2-ethyl-2-hydroxy) butyl-3- (cis-propen-1-yl)- pyrrolidine-5-carboxylic Acid; (2R, 3S, 5R, 1'R, 2'S)-2- (1-Acetamido-2-hydroxy-2-methyl) pentyl-3- (cis-propen-1- yl)-pyrrolidine-5-carboxylic Acid; (2R, 3S, 5R, 1'R, 2'S)-2- (1-Acetamido-2-ethyl-2-hydroxy) pentyl-3- (cis-propen-1-yl)- pyrrolidine-5-carboxylic Acid ; (2R, 3S, 5R, 1'R, 2'S)-2-(1-Acetamido-2-hyd roxy) pentyl-3-(cis-propen-1-yl)- pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt; (2R, 3R, 5R, 1'R, 2'R)-2- (1-Acetamido-2, 3-dihydroxy) propyl-3- (cis-propen-1-yl)- pyrrolidine-5-carboxylic Acid ; (2R, 3S, 5R, 1'R, 2'S)-2- (1-Acetamido-2-hydroxy-4-vinyl) butyl-3- (cis-propen-1-yl)- pyrrolidine-5-carboxylic Acid;.

(2R, 3S, 5R, 1'S)-2-(1-Acetamido-2-hyd roxy) butyl-3-(cis-propen-1-yl)-pyrrolidine-5- carboxylate Ammonium Salt ; (2R, 3S, 5R, 1'R, 2'R)-2- (1-Acetamido-2, 3-dimethoxy) propyl-3- (cis-propen-1-yl)- pyrrolidine-5-carboxylic ; (2R,3S,5R, 1'R, 2'S)-2- (1-Acetamido-2-methoxy-2-vinyl) ethyl-3- (cis-propen-1-yl)- pyrrolidine-5-carboxylic Acid; (2R, 3S, 5R, 1'S)-2- (1-Acetamido-2-ethyl) butyl-3- (cis-propen-1-yl)-pyrrolidine-5- carboxylic Acid ; <BR> <BR> <BR> <BR> <BR> (2R, 3S, 5R, 1'S, 3'S)-2- (1-Acetamido-2- (N-isopropyl-N-methylamino-N-<BR> <BR> <BR> <BR> <BR> oxide)) ethyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic Acid ; (2R, 3S, 5R, 1'S, 3'S)-2-(1-Acetamido-2-(N-ethyl-N-methylamino-N-oxide))ethyl- 3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic Acid; (2R, 3S, 5R, 1'R, 2'S)-2- (1-Acetamido-2-methoxy) butyl-3- (cis-propen-1-yl)- pyrrolidine-5-carboxylic Acid; (2R, 3S, 5R, 1'R, 2'S)-2- (1-Acetamido-2-methoxy) pentyl-3- (cis-propen-1-yl)- pyrrolidine-5-carboxylic Acid; (2R, 3R, 5R, 1'R, 2'S)-2- (1-Acetamido-2-hydroxy) butyl-3- (pyrazol-3-yl)-pyrrolidine-5- carboxylic Acid; (2R, 3S, 5R, 1'R, 2'S)-2- (1-Acetamido-2-hydroxy) butyl-3- (cis-propen-1-yl)- pyrrolidine-5-carboxylic Acid ; (2R, 3S, 5R, 1'R, 2'R)-2- (1-Acetamido-1- (3, 6-dihydro-2-H-pyran-2-yl)) propyl-3- (cis- propen-1-yl)-pyrrolidine-5-carboxylic Acid ; (2R, 3S, 5R, 1'R, 2'S)-2- (1-Acetamido-2-methoxy-2-allyl) ethyl-3- (cis-propen-1-yl)- pyrrolidine-5-carboxylic Acid; (2R, 3S, 5R, 1'R, 2'S, 3'S)-2-(1-Acetamido-2-hydroxy-3-methyl)pentyl-3-(cis-propen- 1-yl)-pyrrolidine-5-carboxylic Acid; (2R, 3S, 5R, 1'R, 2'S)-2- (1-Acetamido-2-methoxy-4-vinyl) butyl-3- (cis-propen-1-yl)- pyrrolidine-5-carboxylic Acid ;.

(2R, 3S, 5R, 1'R, 2'S)-2- (1-Acetamido-2-hydroxy-3-cyano) propyl-3- (cis-propen-1- yl)-pyrrolidine-5-carboxylic ; (2R, 3S, 5R, 1'R, 2'S)-2- (1-Acetamido-1- (3, 6-dihydro-2-H-pyran-2-yl)) methyl-3- (cis- propen-1-yl)-pyrrolidine-5-carboxylic Acid ; (2R, 3S, 5R, 1'R, 2'S)-2- (1-Acetamido-2, 3-dimethoxy) propyl-3- (cis-propen-1-yl)- pyrrolidine-5-carboxylic Acid; (2R, 3S, 5R, 1'R, 2'S)-2-(1-Acetamido-2-hydroxymethyl-2-hydroxy)pentyl-3-(cis- propen-1-yl)-pyrrolidine-5-carboxylic Acid ; (2R, 3S, 5R, 1'R, 2'S)-2- (l-Acetamido-2-ethoxy) pentyl-3- (cis-propen-1-yl)- pyrrolidine-5-carboxylic Acid; (2R, 3S, 5R, 1'R, 2'S)-2- (1-Acetamido-2-hydroxy-3-dimethyl) butyl-3- (cis-propen-1- yl)-pyrrolidine-5-carboxylic Acid; (2R, 3S, 5R, 1'R, 2'S)-2- (1-Acetamido-2-ethoxy-3-vinyl) propyl-3- (cis-propen-1-yl)- pyrrolidine-5-carboxylic Acid ; (2R, 3S, 5R, 1'R, 2'S)-2- (1-Acetamido-2-hydroxy-2- (propeny-2-yl)) ethyl-3- (cis- propen-1-yl)-pyrrolidine-5-carboxylic Acid ; (2R, 3S, 5R, 1'R, 2'S)-2- (1-Acetamido-2-hydroxy) hexyl-3- (cis-propen-1-yl)- pyrrolidine-5-carboxylic Acid; (2R, 3S, 5R, 1'S)-2-(1-acetamido-3-methyl)butyl-3-(cis-propen-1-yl)-pyrro lidine-5- carboxylic ; (2R, 3S, 5R, 1'R, 2'S)-2-(1-Acetamido-2-hydroxy)butyl-3-vinyl-pyrrolidine-5- carboxylic ; (2R, 3S, 5R, 1'R, 2'S)-2-(1-Acetamido-2-hydroxy)pentyl-3-vinyl-pyrrolidine-5- carboxylic Acid; (2R, 3S, 5R, 1'R, 2'R)-2-(1-Acetamido-2-hyd roxyethyl-2-hyd roxy) pentyl-3-(cis- propen-1-yl)-pyrrolidine-5-carboxylic Acid ; (2R, 3S, 5R, 1'R, 2'R)-2- (1-Acetamido-2-hydroxy) butyl-3-vinyl-pyrrolidine-5- carboxylic Acid ; (2R, 3S, 5R, 1'R, 2'R)-2- (1-Acetamido-2-methoxy) pentyl-3- (cis-propen-1-yl)- pyrrolidine-5-carboxylic Acid ; (2R, 3S, 5R, 1'R, 2'R)-2- (1-Acetamido-2-hydroxy) pentyl-3-vinyl-pyrrolidine-5- carboxylic Acid; (2R, 3S, 5R, 1'R)-2- (1-Acetamido-2-hydroxy) ethyl-3- (cis-propen-1-yl)-pyrrolidine-5- carboxylic Acid; (2R, 3S, 5R, 1'S)-2-(1-acetamido-3-methyl)butyl-3-(cis-2-chloro-vin-1-yl) - pyrrolidine-5-carboxylic Acid; (2R, 3R, 5R, 1'S)-2- (1-acetamido-3-methyl) butyl-3- (pyrazol-3-yl)-pyrrolidine-5- carboxylic Acid; (2R, 3S, 5R, 1'S, 3'R)-2- (1-Acetamido-3-hydroxy) pentyl-3- (cis-propen-1-yl)- pyrrolidine-5-carboxylic Acid; (2R, 3R, 5R, 1'S)-2- (1-Acetamido-3-methyl) butyl-3- (thiazol-4-yl)-pyrrolidine-5- carboxylic Acid; (2R, 3R, 5R, 1'S)-1- t-Butoxycarbonyl-2- (1-acetamido-3-methyl) butyl-3- (thiazol-2- yl)-pyrrolidine-5-carboxylic Acid ; (2R, 3S, 5R, 1'S)-2-(1-Acetamido-3-methyl)butyl-3-vinyl-pyrrolidine-5-car boxylic Acid; (2R, 3S, 5R, 1'S)-2-(1-acetamido-3-methyl)butyl-3-(2,2-difluoro-vin-1-yl) -pyrrolidine- 5-carboxylic Acid; (2R, 3R, 5R, 1'S)-2- (1-acetamido-3-methyl) butyl-3- (pyrazol-3-yl)-pyrrolidine-5- carboxylic ; (2R, 3R, 5R, 1'S)-2- (1-acetamido-3-methyl) butyl-3- (isoxazol-3-yl)-pyrrolidine-5- carboxylic Acid; (2R, 3R, 5R, 1'S)-2- (1-acetamido-3-methyl) butyl-3- (isoxazol-5-yl)-pyrrolidine-5- carboxylic Acid; (2R, 3R, 5R, 1'S)-2- (1-Acetamido-3-methyl) butyl-3- (imidazol-2-yl)-pyrrolidine-5- Carboxylic Acid; (2R, 3R, 5R, 1'S)-2- (1-Acetamido-3-methyl) butyl-3- (imidazol-4-yl)-pyrrolidine-5- carboxylic Acid; (2S, 3R, 5R, 1'S)-2- (1-acetamido-3-methyl) butyl-3-amino-pyrrolidine-5-carboxylic Acid; (2R, 3S, 5R, 1'R, 2'S)-2- (1-Acetamido-2-methyl-2-methoxy) pentyl-3- (cis-propen-1- yl)-pyrrolidine-5-carboxylic Acid; (2R, 3S, 5R, 1'R, 2'S)-2- (1-Acetamido-2-methyl-2-methoxy) pentyl-3- (cis-propen-1- yl)-pyrrolidine-5-carboxylic Acid Ethyl Ester ; (2R, 3S, 5R, 1'R, 2'S)-2- (1-Acetamido-2-vinyl-2-methoxy) pentyl-3- (cis-propen-1-yl)- pyrrolidine-5-carboxylic Acid; (2R, 3S, 5R, 1'R)-2-(1-Acetamido-2-ethyl-2-methoxy)butyl-3-(cis-propen-1- yl)- pyrrolidine-5-carboxylic Acid ; (2R, 3S, 5R, 1'R, 2'S)-2-(1-Acetamido-2-methyl-2,3-dimethoxy)propyl-3-(cis-pro pen- 1-yl)-pyrrolidine-5-carboxylic Acid ; and (2R, 3S, 5R, 1'R, 2'S)-2- (1-Acetamido-2-methyl-2-methoxy) butyl-3- (cis-propen-1-yl)- pyrrolidine-5-carboxylic Acid; or a pharmaceutically acceptable salt, ester or prodrug thereof.

More preferred compounds of the invention include compounds having the indicated relative stereochemistry selected from the group consisting of: ()- (2R, 3S, 5R, 1'R)-2- (1-Acetamido-2-ethyl-2-hydroxy) butyl-3- (cis-propen-1-yl)- pyrrolidine-5-carboxylic Acid; (~)-(2R, 3S, 5R, 1'R, 2'S)-2-(1-Acetamido-2-hydroxy-2-methyl)pentyl-3-(cis-propen- 1-yl)-pyrrolidine-5-carboxylic Acid; ()- (2R, 3S, 5R, 1'R, 2'S)-2- (l-Acetamido-2-ethyl-2-hydroxy) pentyl-3- (cis-propen-l- yl)-pyrrolidine-5-carboxylic Acid; ()- (2R, 3S, 5R, 1'R, 2'S)-2-(1-Acetamido-2-hydroxy)pentyl-3-(cis-propen-1-yl)- pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt ; ()- (2R, 3R, 5R, 1'R, 2'R)-2- (1-Acetamido-2, 3-dihydroxy) propyl-3-(cis-propen-1-yl)- pyrrolidine-5-carboxylic Acid; (i)-(2R, 3S, 5R, 1'R, 2'S)-2-(1-Acetamido-2-hydroxy4-vinyl) butyl-3-(cis-propen-1- yl)-pyrrolidine-5-carboxylic Acid;. <BR> <BR> <BR> <BR> <BR> <P> (-)- (2R, 3S, 5R, 1'S)-2- (1-Acetamido-2-ethyl) butyl-3- (cis-propen-1-yl)-pyrrolidine-5- carboxylate Ammonium Salt ; ()- (2R, 3S, 5R, 1'R, 2'R)-2-(1-Acetamido-2, 3-d imethoxy) propyl-3-(cis-propen-1-yl)- pyrrolidine-5-carboxylic Acid ; (i)-(2R, 3S, 5R, 1'R, 2'S)-2-(1-Acetamido-2-methoxy-2-vinyl)ethyl-3-(cis-propen-1- yl)-pyrrolidine-5-carboxylic Acid; (~)- (2R, 3S, 5R, 1'S)-2- (1-Acetamido-2-ethyl) butyl-3- (cis-propen-1-yl)-pyrrolidine-5- carboxylic Acid; ()- (2R, 3S, 5R, 1'S, 3'S)-2-(1-Acetamido-2-(N-isopropyl-N-methylamino-N- oxide)) ethyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic Acid; ()- (2R, 3S, 5R, 1'S, 3'S)-2-(1-Acetamido-2-(N-ethyl-N-methylamino-N-oxide))ethyl- 3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic ; ()- (2R, 3S, 5R, 1'R, 2'S)-2- (1-Acetamido-2-methoxy) butyl-3- (cis-propen-1-yl)- pyrrolidine-5-carboxylic Acid ; ()- (2R, 3S, 5R, 1'R, 2'S)-2- (1-Acetamido-2-methoxy) pentyl-3- (cis-propen-1-yl)- pyrrolidine-5-carboxylic Acid; ()- (2R, 3R, 5R, 1'R, 2'S)-2- (1-Acetamido-2-hydroxy) butyl-3- (pyrazol-3-yl)- pyrrolidine-5-carboxylic ; ()- (2R, 3S, 5R, 1'R, 2'S)-2- (1-Acetamido-2-hydroxy) butyl-3- (cis-propen-1-yl)- pyrrolidine-5-carboxylic Acid; ()- (2R, 3S, 5R, 1'R, 2'R)-2-(1-Acetamido-1-(3, 6-dihyd ro-2-H-pyran-2-yl)) propyl-3- (cis-propen-1-yl)-pyrrolid ine-5-carboxylic Acid; (~)-(2R, 3S, 5R, 1'R, 2'S)-2- (1-Acetamido-2-methoxy-2-allyl) ethyl-3- (cis-propen-1- yl)-pyrrolidine-5-carboxylic Acid; ()- (2R, 3S, 5R, 1'R, 2'S, 3'S)-2-(1-Acetamido-2-hydroxy-3-methyl)pentyl-3-(cis- propen-1-yl)-pyrrolidine-5-carboxylic Acid ; ()- (2R, 3S, 5R, 1'R, 2'S)-2-(1-Acetamido-2-methoxy-4-vinyl)butyl-3-(cis-propen-1- yl)-pyrrolidine-5-carboxylic Acid ; ()- (2R, 3S, 5R, 1'R, 2'S)-2-(1-Acetamido-2-hydroxy-3-cyano)propyl-3-(cis-propen- 1-yl)-pyrrolidine-5-carboxylic Acid ; ()- (2R, 3S, 5R, 1'R, 2'S)-2- (1-Acetamido-1- (3, 6-dihydro-2-H-pyran-2-yl)) methyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic Acid; ()- (2R, 3S, 5R, 1'R, 2'S)-2- (1-Acetamido-2, 3-dimethoxy) propyl-3- (cis-propen-1-yl)- pyrrolidine-5-carboxylic Acid; ()- (2R, 3S, 5R, 1'R, 2'S)-2- (1-Acetamido-2-hydroxymethyl-2-hydroxy) pentyl-3- (cis- propen-1-yl)-pyrrol idine-5-carboxylic Acid ; ()- (2R, 3S, 5R, 1'R, 2'S)-2- (1-Acetamido-2-ethoxy) pentyl-3- (cis-propen-1-yl)- pyrrolidine-5-carboxylic Acid; ()- (2R, 3S, 5R, 1'R, 2'S)-2-(1-Acetamido-2-hydroxy-3-dimethyl)butyl-3-(cis-propen - 1-yl)-pyrrolidine-5-carboxylic Acid; (~)- (2R, 3S, 5R, 1'R, 2'S)-2-(1-Acetamido-2-ethoxy-3-vinyl)propyl-3-(cis-propen-1- yl)-pyrrolidine-5-carboxylic Acid; ()- (2R, 3S, 5R, 1'R, 2'S)-2- (1-Acetamido-2-hydroxy-2- (propeny-2-yl)) ethyl-3- (cis- propen-1-yl)-pyrrolidine-5-carboxylic Acid ; ()- (2R, 3S, 5R, 1'R, 2'S)-2-(1-Acetamido-2-hydroxy) hexyl-3-(cis-propen-1-yl)- pyrrolidine-5-carboxylic Acid; ()- (2R, 3S, 5R, 1'R, 2'S)-2-(1-Acetamido-2-methyl-2-methoxy)pentyl-3-(cis-propen- 1-yl)-pyrrolidine-5-carboxylic Acid; ()- (2R, 3S, 5R, 1'R, 2'S)-2-(1-Acetamido-2-methyl-2-methoxy)pentyl-3-(cis-propen- 1-yl)-pyrrolidine-5-carboxylic Acid Ethyl Ester; ()- (2R, 3S, 5R, 1'R, 2'S)-2-(1-Acetamido-2-vinyl-2-methoxy)pentyl-3-(cis-propen-1 - yl)-pyrrolidine-5-carboxylic Acid; (~)-(2R, 3S, 5R, 1'R)-2- (1-Acetamido-2-ethyl-2-methoxy) butyl-3- (cis-propen-1-yl)- pyrrolidine-5-carboxylic Acid; ()- (2R, 3S, 5R, 1'R, 2'S)-2-(1-Acetamido-2-methyl-2, 3-dimethoxy) propyl-3-(cis- propen-1-yl)-pyrrolidine-5-carboxylic Acid ; and ()- (2R, 3S, 5R, 1'R, 2'S)-2-(1-Acetamido-2-methyl-2-methoxy)butyl-3-(cis-propen- 1-yl)-pyrrolidine-5-carboxylic Acid; or a pharmaceutically acceptable salt, ester or prodrug thereof.

Other more preferred compounds of the invention include enantiomerically enriched compounds having the indicated absolute stereochemistry selected from the group consisting of: (2R, 3S, 5R, 1'R)-2- (1-Acetamido-2-ethyl-2-hydroxy) butyl-3- (cis-propen-1-yl)- pyrrolidine-5-carboxylic Acid; (2R, 3S, 5R, 1'R, 2'S)-2-(1-Acetamido-2-hydroxy-2-methyl) pentyl-3-(cis-propen-1- yl)-pyrrolidine-5-carboxylic Acid; (2R, 3S, 5R, 1'R,2'S)-2-(1-Acetamido-2-ethyl-2-hydroxy)pentyl-3-(cis-prop en-1-yl)- pyrrolidine-5-carboxylic Acid; (2R, 3S, 5R, 1'R, 2'S)-2- (1-Acetamido-2-hydroxy) pentyl-3- (cis-propen-1-yl)- pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt ; (2R, 3R, 5R, 1'R, 2'R)-2-(1-Acetamido-2,3-dihydroxy)propyl-3-(cis-propen-1-yl) - pyrrolidine-5-carboxylic ; (2R, 3S, 5R, 1,R,2'S)-2-(1-Acetamido-2-hydroxy-4-vinyl)butyl-3-(cis-prope n-1-yl)- pyrrolidine-5-carboxylic Acid;.

(-)- (2R, 3S, 5R, 1'S)-2- (1-Acetamido-2-ethyl) butyl-3- (cis-propen-1-yl)-pyrrolidine-5- carboxylate Ammonium Salt ; (2R, 3S, 5R, 1'R, 2'R)-2- (1-Acetamido-2, 3-dimethoxy) propyl-3-(cis-propen-1-yl)- pyrrolidine-5-carboxylic Acid; (2R, 3S, 5R, 1'R, 2'S)-2- (1-Acetamido-2-methoxy-2-vinyl) ethyl-3- (cis-propen-1-yl)- pyrrolidine-5-carboxylic Acid; (2R, 3S, 5R, 1'S)-2- (1-Acetamido-2-ethyl) butyl-3- (cis-propen-1-yl)-pyrrolidine-5- carboxylic Acid; (2R, 3S, 5R, 1'S, 3'S)-2-(1-Acetamido-2-(N-isopropyl-N-methylamino-N- oxide)) ethyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylicAcid; (2R, 3S, 5R, 1'S, 3'S)-2-(1-Acetamido-2-(N-ethyl-N-methylamino-N-oxide))ethyl- 3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic Acid; (2R, 3S, 5R, 1'R, 2'S)-2- (1-Acetamido-2-methoxy) butyl-3- (cis-propen-1-yl)- pyrrolidine-5-carboxylic Acid; (2R, 3S, 5R, 1'R, 2'S)-2-(1-Acetamido-2-methoxy) pentyl-3-(cis-propen-1-yl)- pyrrolidine-5-carboxylic Acid; (2R, 3R, 5R, 1'R, 2'S)-2-(1-Acetamido-2-hydroxy) butyl-3-(pyrazol-3-yl)-pyrrolidine-5- carboxylic Acid; (2 R, 3S, 5R, 1'R,2'S)-2-(1-Acetamido-2-hydroxy)butyl-3-(cis-propen-1-yl)- pyrrolidine-5-carboxylic ; (2R, 3S, 5R, 1'R, 2'R)-2- (1-Acetamido-1- (3, 6-dihydro-2-H-pyran-2-yl)) propyl-3- (cis- propen-1-yi)-pyrrolidine-5-carboxylic Acid (2R, 3S, 5R, 1'R, 2'S)-2- (1-Acetamido-2-methoxy-2-allyl) ethyl-3- (cis-propen-1-yl)- pyrrolidine-5-carboxylic Acid ; (2R, 3S, 5R, 1'R, 2'S, 3'S)-2-(1-Acetamido-2-hydroxy-3-methyl)pentyl-3-(cis-propen- 1-yl)-pyrrolidine-5-carboxylic ; (2R, 3S, 5R, 1'R, 2'S)-2- (1-Acetamido-2-methoxy-4-vinyl) butyl-3- (cis-propen-1-yl)- pyrrolidine-5-carboxylic Acid ; (2R, 3S, 5R, 1'R, 2'S)-2-(1-Acetamido-2-hydroxy-3-cyano)propyl-3-(cis-propen-1 - yl)-pyrrolidine-5-carboxylic Acid; (2R, 3S, 5R, 1'R, 2'S)-2- (1-Acetamido-1- (3, 6-dihydro-2-H-pyran-2-yl)) methyl-3- (cis- propen-1-y !)-pyrro) idine-5-carboxy) ic Acid ; (2R, 3S, 5R, 1'R, 2'S)-2-(1-Acetamido-2,3-dimethoxy)propyl-3-(cis-propen-1-yl) - pyrrolidine-5-carboxylic Acid; (2R, 3S, 5R, 1'R, 2'S)-2- (1-Acetamido-2-hydroxymethyl-2-hydroxy) pentyl-3- (cis- propen-1-y))-pyrro ! idine-5-carboxy ! ic Acid ; (2R, 3S, 5R, 1'R, 2'S)-2-(1-Acetamido-2-ethoxy)pentyl-3-(cis-propen-1-yl)- pyrrolidine-5-carboxylic Acid; (2R, 3S, 5R, 1'R, 2'S)-2- (1-Acetamido-2-hydroxy-3-dimethyl) butyl-3- (cis-propen-1- yl)-pyrrolidine-5-carboxylic Acid ; (2R, 3S, 5R, 1'R, 2'S)-2-(1-Acetamido-2-ethoxy-3-vinyl)propyl-3-(cis-propen-1- yl)- pyrrolidine-5-carboxylic ; (2R, 3S, 5R, 1'R, 2'S)-2-(1-Acetamido-2-hydroxy-2-(propeny-2-yl))ethyl-3-(cis- propen-1-yl)-pyrrolidine-5-carboxylic Acid ; (2R, 3S, 5R, 1'R, 2'S)-2- (1-Acetamido-2-hydroxy) hexyl-3- (cis-propen-1-yl)- pyrrolidine-5-carboxylic Acid; (2R, 3S, 5R, 1'R, 2'S)-2-(1-Acetamido-2-methyl-2-methoxy) pentyl-3-(cis-propen-1- yl)-pyrrolidine-5-carboxylic Acid ; (2R, 3S, 5R, 1'R, 2'S)-2- (1-Acetamido-2-methyl-2-methoxy) pentyl-3- (cis-propen-1- yl)-pyrrolidine-5-carboxylic Acid Ethyl Ester; (2R, 3S, 5R, 1'R, 2'S)-2- (1-Acetamido-2-vinyl-2-methoxy) pentyl-3- (cis-propen-1-yl)- pyrrolidine-5-carboxylic Acid ; (2R, 3S, 5R, 1'R)-2- (1-Acetamido-2-ethyl-2-methoxy) butyl-3- (cis-propen-1-yl)- pyrrolidine-5-carboxylic Acid; (2R, 3S, 5R, 1'R, 2'S)-2-(1-Acetamido-2-methyl-2, 3-dimethoxy) propyl-3- (cis-propen- 1-yl)-pyrrolidine-5-carboxylic Acid ; and (2R, 3S, 5R, 1'R, 2'S)-2-(1-Acetamido-2-methyl-2-methoxy)butyl-3-(cis-propen-1 -yl)- pyrrolidine-5-carboxylic ; or a pharmaceutically acceptable salt, ester or prodrug thereof.

Even more preferred compounds of the invention include compounds having the indicated relative stereochemistry selected from the group consisting of : ()- (2R, 3S, 5R, 1'R,2'S)-2-(1-Acetamido-2-methyl-2-methoxy)pentyl-3-(cis-pro pen- 1-yl)-pyrrolidine-5-carboxylic Acid; ()- (2R, 3S, 5R, 1'R, 2'S)-2-(1-Acetamido-2-methyl-2-methoxy)pentyl-3-(cis-propen- 1-yl)-pyrrolidine-5-carboxylic Acid Ethyl Ester ; ()- (2R, 3S, 5R, 1'R, 2'S)-2-(1-Acetamido-2-vinyl-2-methoxy)pentyl-3-(cis-propen-1 - yl)-pyrrolidine-5-carboxylic Acid; <BR> <BR> <BR> <BR> <BR> (ffi)-(2R, 3S, 5R, 1'R)-2-(1-Acetamido-2-ethyl-2-methoxy) butyl-3-(cis-propen-1-yl)- pyrrolidine-5-carboxylic Acid ; ()- (2R, 3S, 5R, 1'R, 2'S)-2-(1-Acetamido-2-methyl-2,3-dimethoxy)propyl-3-(cis- propen-1-yl)-pyrrolidine-5-carboxylic Acid ; and ()- (2R, 3S, 5R, 1'R, 2'S)-2-(1-Acetamido-2-methyl-2-methoxy) butyl-3-(cis-propen- 1-yl)-pyrrolidine-5-carboxylic Acid; or a pharmaceutically acceptable salt, ester or prodrug thereof.

Other even more preferred compounds of the invention include enantiomerically enriched compounds having the indicated absolute stereochemistry selected from the group consisting of: (-)- (2R, 3S, 5R, 1'R, 2'S)-2- (1-Acetamido-2-methyl-2-methoxy) pentyl-3- (cis-propen- 1-yl)-pyrrolidine-5-carboxylic Acid; (-)- (2R, 3S, 5R, 1'R, 2'S)-2- (1-Acetamido-2-methyl-2-methoxy) pentyl-3- (cis-propen- 1-yl)-pyrrolidine-5-carboxylic Acid Ethyl Ester; (2R, 3S, 5R, 1'R, 2'S)-2-(1-Acetamido-2-vinyl-2-methoxy)pentyl-3-(cis-propen-1 -yl)- pyrrolidine-5-carboxylic Acid ; (2R, 3S, 5R, 1'R)-2-(1-Acetamido-2-ethyl-2-methoxy)butyl-3-(cis-propen-1- yl)- pyrrolidine-5-carboxylic Acid; (2R, 3S, 5R, 1'R, 2'S)-2-(1-Acetamido-2-methyl-2,3-dimethoxy)propyl-3-(cis-pro pen- 1-yl)-pyrrolidine-5-carboxylic Acid ; and (2R, 3S, 5R, 1'R, 2'S)-2-(1-Acetamido-2-methyl-2-methoxy)butyl-3-(cis-propen-1 -yl)- pyrrolidine-5-carboxylic Acid; or a pharmaceutically acceptable salt, ester or prodrug thereof.

Most highly preferred compounds of the invention include enantiomerically enriched esters or prodrugs of compounds having the indicated absolute stereochemistry selected from the group consisting of: (-)-(2R, 3S, 5R, 1'R, 2'S)-2- (1-Acetamido-2-methyl-2-methoxy) pentyl-3- (cis-propen- 1-yl)-pyrrolidine-5-carboxylic ; (2R, 3S, 5R, 1'R, 2'S)-2- (1-Acetamido-2-vinyl-2-methoxy) pentyl-3- (cis-propen-1-yl)- pyrrolidine-5-carboxylic Acid; (2R, 3S, 5R, 1'R)-2- (1-Acetamido-2-ethyl-2-methoxy) butyl-3- (cis-propen-1-yl)- pyrrolidine-5-carboxylic Acid; (2R, 3S, 5R, 1'R, 2'S)-2- (1-Acetamido-2-methyl-2, 3-dimethoxy) propyl-3- (cis-propen- 1-yl)-pyrrolidine-5-carboxylic Acid ; and (2R, 3S, 5R, 1'R, 2'S)-2-(1-Acetamido-2-methyl-2-methoxy) butyl-3-(cis-propen-1-yl)- pyrrolidine-5-carboxylic Acid; or a pharmaceutically acceptable salt thereof.

Most highly preferred compounds of the invention also include enantiomerically enriched esters or prodrugs of the compound having the indicated absolute stereochemistry: (-)- (2R, 3S, 5R, 1'R, 2'S)-2-(1-Acetamido-2-methyl-2-methoxy) pentyl-3-(cis-propen- 1-yl)-pyrrolidine-5-carboxylic Acid; or a pharmaceutically acceptable salt thereof.

The term"acid protecting groupas used herein refers to groups used to protect acid groups (for example,-C02H,-S03H,-S02H,-P03H2,-PO2H groups and the like) against undesirable reactions during synthetic procedures.

Commonly used acid protecting groups are disclosed in T. H. Greene and P. G. M.

Wuts, Protective Groups in Organic Svnthesis. 2nd edition, John Wiley & Sons, New York (1991) which is incorporated herein by reference. Most frequently, such acid protecting groups are esters.

Such esters include : alkyl esters, especially loweralkyl esters, including, but not limited to, ethyl, n-propyl, isopropyl, n-butyl, sec-butyl, isobutyl, t-butyl, n-pentyl esters and the like ; arylalkyl esters including, but not limited to, benzyl, phenethyl, 3- phenylpropyl, naphthylmethyl esters and the like, wherein the aryl part of the arylalkyl group is unsubstituted or substituted as previously defined herein; silylesters, especially, (tri-loweralkyl) silyl esters, (di-loweralkyl) (aryl) silyl esters and (loweralkyl) (di-aryl) silyl esters, including, but not limited to, <BR> <BR> <BR> trimethylsilyl, triethylsilyl, isopropyidimethylsilyl, t-butyidimethylsilyl,<BR> <BR> <BR> <BR> methyldiisopropylsilyl, methyldi-t-butylsilyl, triisopropylsilyl, methyidiphenylsilyl, isopropyldiphenylsilyl, butyidiphenylsilyl, phenyidiisopropylsilyl esters and the like ; and the like.

Preferred acid protecting groups are loweralkyl esters.

The term"activated carboxylic acid group"as used herein refers to acid halides such as acid chlorides and also refers to activated ester derivatives including, but not limited to, formic and acetic acid derived anhydrides, anhydrides derived from alkoxycarbonyl halides such as isobutyloxycarbonylchloride and the like, anhydrides derived from reaction of the carboxylic acid with N, N'-carbonyldiimidazole and the like, N-hydroxysuccinimide derived esters, N-hydroxyphthalimide derived esters, N-hydroxybenzotriazole derived esters, N-hydroxy-5-norbornene-2, 3-dicarboximide derived esters, 2,4,5- trichlorophenol derived esters, p-nitrophenol derived esters, phenol derived esters, pentachlorophenol derived esters, 8-hydroxyquinoline derived esters and the like.

The term"acyl"as used herein, refers to groups having the formula -C (=O)-R95 wherein R95 is hydrogen or an alkyl group. Preferred alkyl groups as R95 are loweralkyl groups. Representative examples of acyl groups include groups such as, for example, formyl, acetyl, propionyl, and the like.

The term"acylalkyl"as used herein refers to an acyl group appended to an alkyl radical. Representative examples of acylalkyl groups include acetylmethyl, acetylethyl, propionylmethyl, propionylethyl and the like.

The term"acylamino"as used herein, refers to groups having the formula -NHR89 wherein R89 is an acyl group. Representative examples of acylamino include acetylamino, propionylamino, and the like.

The term"acyloxyalkyl"as used herein refers to an acyloxy group (i. e., R95-C (O)-O- wherein R95 is hydrogen or an alkyl group) which is appended to an alkyl radical. Representative examples of acyloxyalkyl include acetyloxymethyl, acetyloxyethyl, propioyloxymethyl, propionyloxyethyl and the like.

The term"alkenyl"as used herein, refers to a straight or branched chain hydrocarbon radical containing from 2 to 15 carbon atoms and also containing at least one carbon-carbon double bond. The term"lower alkenyl"refers to straight or branched chain alkenyl radicals containing from 2 to 6 carbon atoms.

Representative examples of alkenyl groups include groups such as, for example, vinyl, 2-propenyl, 2-methyl-1-propenyl, 3-butenyl, 4-pentenyl, 5-hexenyl and the like.

The term"alkenylene"as used herein, refers to a divalent group derived from a straight or branched chain hydrocarbon containing from 2 to 15 carbon atoms and also containing at least one carbon-carbon double bond. The term "lower alkenylene"refers to a divalent group derived from a straight or branched chain alkene group having from 2 to 6 carbon atoms. Representative examples of alkenylene groups include groups such as, for example,-CH=CH-, -CH2CH=CH-,-C (CH3) =CH-,-CH2CH=CHCH2-, and the like.

The term"alkenyloxy"as used herein, refers to groups having the formula -oR8'where R81 is an alkenyl group.

The term"alkoxy"as used herein, refers to groups having the formula -OR99 wherein R99 is an alkyl group. Preferred R99 groups are loweralkyl groups.

Representative examples of alkoxy groups include groups such as, for example, methoxy, ethoxy, tert-butoxy, and the like.

The term"alkoxyalkoxy"as used herein, refers to groups having the formula-o-R96-o-R97 wherein R97 is loweralkyl, as defined herein, and R96 is a lower alkylene group. Representative examples of alkoxyalkoxy groups include groups such as, for example, methoxymethoxy, ethoxymethoxy, t-butoxymethoxy and the like.

The term"alkoxyalkyl"as used herein refers to an alkyl radical to which is appended an alkoxy group, for example, methoxymethyl, methoxylpropyl and the like.

The term"alkoxycarbonyl"as used herein, refers to groups having the formula,-C (=O)- R80, where R80 is an alkoxy group.

The term"alkoxycarbonylalkyl"as used herein, refers to groups having the formula (=O)- R79, appended to the parent molecular moiety through an alkylene linkage, where R79 is an alkoxy group.

The term"alkoxycarbonyloxyalkyl"as used herein refers to an alkoxycarbonyloxy group (i. e., R80-C (O)-O wherein R80 is an alkoxy group) appended to an alkyl radical. Representative examples of alkoxycarbonyloxyalkyl include methoxycarbonyloxymethyl, ethoxycarbonyloxymethyl, methoxycarbonyloxyethyl and the like.

As used herein, the term"alkyl"refers to straight or branched chain hydrocarbon radicals containing from 1 to 12 carbon atoms. The term "loweralkyl"refers to straight or branched chain alkyl radicals containing from 1 to 6 carbon atoms. Representative examples of alkyl groups include groups such as, for example, methyl, ethyl, n-propyl, iso-propyl, n-butyl, iso-butyl, sec-butyl, t-butyl n-pentyl, 1-methylbutyl, 2,2-dimethylbutyl, 2-methylpentyl, 2,2-dimethyl- propyl, n-hexyl, and the like. The hydrocarbon chains in alkyl groups or the alkyl portion of an alkyl-containing substituent can be optionally interrupted by one or two heteroatoms or heterogroups independently selected from the group consisting of oxygen,-N (R27)-and sulfur wherein R27 at each occurrence is independently hydrogen, loweralkyl, cylcoalkyl, cycloalkylalkyl or arylalkyl and wherein two such heteroatoms or heterogroups are separated by at least one carbon atom.

The term"alkylamino"as used herein, refers to groups having the formula -NHR9'wherein R9'is an alkyl group. Preferred R9 groups are loweralkyl groups. Representative examples of alkylamino include methylamino, ethylamino, and the like.

The term"alkylene"as used herein, refers to a divalent group derived from a straight or branched chain saturated hydrocarbon group having from 1 to 15 carbon. The term"lower alkylene"refers to a divalent group derived from a straight or branched chain saturated hydrocarbon group having from 1 to 6 carbon atoms. Representative examples of alkylene groups include groups such as, for example, methylene (-CH2-), 1,2-ethylene (-CH2CH2-), 1,1-ethylene (-CH (CH3)-), 1, 3-propylene (-CH2CH2CH2-), 2,2-dimethylpropylene (-CH2C (CH3) 2CH2-), and the like. The hydrocarbon chains in alkylene groups or the alkylene portion of an alkylene-containing substituent can be optionally interrupted by one or two heteroatoms or heterogroups independently selected from the group consisting of oxygen,-N (R27)-and sulfur wherein R27 at each occurrence is independently hydrogen, loweralkyl, cylcoalkyl, cycloalkylalkyl or arylalkyl and wherein two such heteroatoms or heterogroups are separated by at least one carbon atom.

The term"alkylsulfonyl"as used herein refers to the group having the formula,-S02-R, where R is an alkyl group. Preferred groups R are loweralkyl groups.

The term"alkylsulfonylamino"as used herein refers to the group having the formula,-S02-R, appended to the parent molecular moiety through an amino linkage (-NH-), where R is an alkyl group. Preferred groups R77 are loweralkyl groups.

The term"alkynyl"as used herein, refers to a straight or branched chain hydrocarbon radical containing from 2 to 15 carbon atoms and also containing at least one carbon-carbon triple bond. The term"lower alkynyl"refers to straight or branched chain alkynyl radicals containing from 2 to 6 carbon atoms.

Representative examples of alkynyl groups include groups such as, for example, acetylenyl, 1-propynyl, 2-propynyl, 3-butynyl, 2-pentynyl, 1-butynyl and the like.

The term"alkynylene"as used herein, refers to a divalent group derived from a straight or branched chain hydrocarbon containing from 2 to 15 carbon atoms and also containing at least one carbon-carbon triple bond. The term "lower alkynylene"refers to a divalent group derived from a straight or branched chain alkynylene group from 2 to 6 carbon atoms. Representative examples of alkynylene groups include groups such as, for example, -C#C-, -CH2-C#C-, -C=-C-CH2-,-CH (CH3)-C=C-, and the like.

The term"aminoalkyl"as used herein refers to an alkyl radical to which is appended an amino (-NH2) group.

The term"aryl"as used herein refers to a carbocyclic ring system having 6-10 ring atoms and one or two aromatic rings. Representative examples of aryl groups include groups such as, for example, phenyl, naphthyl, tetrahydronaphthyl, indanyl, indenyl and the like.

The aryl groups can be unsubstituted or substituted with one, two or three substituents, each independently selected from loweralkyl, halo, haloalkyl, haloalkoxy, hydroxy, oxo (=O), hydroxyalkyl, alkenyloxy, alkoxy, alkoxyalkoxy, alkoxycarbonyl, alkoxycarbonylalkyl, thioalkoxy, amino, alkylamino, alkylsulfonyl, dialkylamino, acylamino, unsubstituted aryl, unsubstituted arylalkyl, unsubstituted arylalkoxy, unsubstituted aryloxy, mercapto, cyano, nitro, carboxy, carboxaldehyde, NH2C (=O)-, cycloalkyl, carboxyalkyl, alkylsulfonylamino, unsubstituted heterocyclic, unsubstituted (heterocyclic) alkyl, unsubstituted (heterocyclic) alkoxy, unsubstituted (heterocyclic) oxy and-SO3H. Preferred aryi substituents are each independently selected from the group consisting of loweralkyl, halo, haloalkyl, hydroxy, hydroxyalkyl, alkenyloxy, alkoxy, alkoxyalkoxy, thioalkoxy, amino, alkylamino, dialkylamino, alkylsulfonyl, acylamino, cyano and nitro. Examples of substituted aryl include 3-chlorophenyl, 3-fluorophenyl, 4-chlorophenyl, 4-fluorophenyl, 3,4-dichlorophenyl, 3-chloro-4-fluoro-phenyl, 4-methylsulfonylphenyl, and the like.

The term" (aryl) alkenyl" refers to a lower alkenyl group having appended thereto an aryl group. Representative examples of (aryl) alkenyl groups include groups such as, for example phenylethylenyl,-phenylpropenyl, and the like.

The term" (aryl) alkyl" refers to a loweralkyl group having appended thereto an aryl group. Representative examples of (aryl) alkyl groups include groups such as, for example benzyl and phenylethyl.

The term"arylalkoxy"as used herein refers to the group having the formula, o-R76 where R76 is an arylalkyl group.

The term" (aryl) alkynyl" refers to an alkynylene group having appended thereto an aryl group. Representative examples of (aryl) alkynyl groups include groups such as, for example phenylacetylenyl, phenylpropynyl, and the like.

The term"aryloxy"as used herein refers to the group having the formula, -0-R, where R is an aryl group.

The term"carbamoyl"as used herein refers to the group having the formula (=O)-NH2.

The term"carboxyalkyl"as used herein, refers to the group having the formula,-R64-COOH, where R64 is a lower alkylene group.

The term"cyanoalkyl"as used herein refers to an alkyl radical to which is appended a cyano group (-CN).

The term"cycloalkenyl"as used herein refers to an aliphatic ring system having 5 to 10 carbon atoms and 1 or 2 rings containing at least one double bond in the ring structure. Representative examples of cycloalkenyl groups include groups such as, for example, cyclohexene, cyclopentene, norbornene and the like.

Cycloalkenyl groups can be unsubstituted or substituted with one, two or three substituents independently selected hydroxy, halo, amino, alkylamino, dialkylamino, alkoxy, alkoxyalkoxy, thioalkoxy, haloalkyl, mercapto, loweralkenyl and loweralkyl. Preferred substitutents are independently selected from loweralkyl, loweralkenyl, haloalkyl, halo, hydroxy and alkoxy.

The term" (cycloalkenyl) alkenyl" as used herein refers to a cycloalkenyl group appended to a lower alkenyl radical. Representative examples of (cycloalkenyl) alkenyl groups include groups such as, for example, cyclohexenylethylene, cyclopentenylethylene, and the like.

The term" (cycloalkenyl) alkyl" as used herein refers to a cycloalkenyl group appended to a lower alkyl radical. Representative examples of (cycloalkenyl) alkyl groups include groups such as, for example, cyclohexenylmethyl, cyclopentenylmethyl, cyclohexenylethyl, cyclopentenylethyl, and the like.

The term" (cycloalkenyl) alkynyl" as used herein refers to a cycloalkenyl group appended to a lower alkynyl radical. Representative examples of (cycloalkenyl) alkynyl groups include groups such as, for example, cyclohexenylacetylenyl, cyclopentenylpropynyl, and the like.

The term"cycloalkyl"as used herein refers to an aliphatic ring system having 3 to 10 carbon atoms and 1 or 2 rings. Representative cylcoalkyl groups include, for example, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, norbornane, bicyclo [2.2.2] octane and the like.

Cycloalkyl groups can be unsubstituted or substituted with one, two or three substituents independently selected hydroxy, halo, amino, alkylamino, dialkylamino, alkoxy, alkoxyalkoxy, thioalkoxy, haloalkyl, mercapto, loweralkenyl and loweralkyl. Preferred substitutents are independently selected from loweralkyl, loweralkenyl, haloalkyl, halo, hydroxy and alkoxy.

The term" (cycloalkyl) alkyl" as used herein refers to a cycloalkyl group appended to a loweralkyl radical. Representative examples of (cycloalkyl) alkyl groups include groups such as, for example, cyclohexylmethyl, cyclopentylmethyl, cyclohexylethyl, cyclopentylethyl, and the like.

The term" (cycloalkyl) alkenyl" as used herein refers to a cycloalkyl group appended to a lower alkenyl radical. Representative examples of (cycloalkyl)- alkenyl groups include groups such as, for example, cyclohexylethylene, cyclopentylethylene, and the like.

The term" (cycloalkyl) alkynyl" as used herein refers to a cycloalkyl group appended to a lower alkynyl radical. Representative examples of (cycloalkyl)- alkynyl groups include groups such as, for example, cyclohexylacetylenyl, cyclopentylpropynyl, and the like.

The term"dialkylamino"as used herein, refers to groups having the formula-N (R9°) 2 wherein each R90 is independently a lower alkyl group.

Representative examples of dialkylamino include dimethylamino, diethylamino, N- methyl-N-isopropylamino and the like.

The term"dialkylaminoalkyl"as used herein refers to a dialkylamino group appended to an alkyl radical. Representative examples of dialkylaminoalkyl include dimethylaminomethyl, dimethylaminoethyl, N-methyl-N-ethylaminoethyl and the like.

The term"dialkylaminocarbonylalkyl"as used herein refers to a -C (O)-N (R9°) 2 group (wherein each R90 is independently a lower alkyl group) appended to an alkyl radical. Representative examples of dialkylaminocarbonylalkyl include dimethylaminocarbonylmethyl, diethylaminocarbonylmethyl, N-methyl-N-ethylaminocarbonylethyl and the like.

The term"dialkylaminocarbonyloxyalkyl"as used herein refers to a -O-C (O)-N (R9°) 2 group (wherein each R90 is independently a lower alkyl group) appended to an alkyl radical. Representative examples of dialkylaminocarbonyloxyalkyl include dimethylaminocarbonyloxymethyl, diethylaminocarbonyloxymethyl, N-methyl-N-ethylaminocarbonyloxyethyl and the like.

The term"enantiomerically enriched"as used herein refers to a compound which comprises unequal amounts of the enantiomers of an enantiomeric pair. In other words, an enantiomerically enriched compound comprises more than 50% of one enantiomer of an enantiomeric pair and less than 50% of the other enantiomer of the enantiomeric pair. Preferably, a compound that is enantiomerically enriched comprises predominantly one enantiomer of an enantiomeric pair. Preferably, an enantiomerically enriched compound comprises greater than 80% of one enantiomer of an enantiomeric pair and less than 20% of the other enantiomer of the enantiomeric pair. More preferably, an enantiomerically enriched compound comprises greater than 90% of one enantiomer of an enantiomeric pair and less than 10% of the other enantiomer of the enantiomeric pair. Even more preferably, an enantiomerically enriched compound comprises greater than 95% of one enantiomer of an enantiomeric pair and less than 5% of the other enantiomer of the enantiomeric pair. Even more highly preferably, an enantiomerically enriched compound comprises greater than 97% of one enantiomer of an enantiomeric pair and less than 3% of the other enantiomer of the enantiomeric pair. Yet even more highly preferably, an enantiomerically enriched compound comprises greater than 98% of one enantiomer of an enantiomeric pair and less than 2% of the other enantiomer of the enantiomeric pair. Most preferably, an enantiomerically enriched compound comprises greater than 99% of one enantiomer of an enantiomeric pair and less than 1 % of the other enantiomer of the enantiomeric pair.

The term"halo"or"halide"as used herein refers to F, Cl, Br or 1.

The term"haloalkenyl"as used herein refers to a loweralkenyl group in which one or more hydrogen atoms is replaced with a halogen. Examples of haloalkenyl groups include 2-fluoroethylene, 1-chloroethylene, 1,2- difluoroethylene, trifluoroethylene, 1,1,1-trifluoro-2-propylene and the like.

The term"haloalkoxy"as used herein refers to the group having the formula,-OR, where R69 is a haloalkyl group as defined herein. Examples of haloalkoxy include chloromethoxy, fluoromethoxy, dichloromethoxy, trifluoromethoxy and the like.

The term"haloalkyl"as used herein, refers to a loweralkyl group in which one or more hydrogen atoms has been replaced with a halogen including, but not limited to, trifluoromethyl, trichloromethyl, difluoromethyl, dichloromethyl, fluoromethyl, chloromethyl, chloroethyl, 2,2-dichloroethyl, 2,2,2-trichloroethyl, pentafluoroethyl and the like.

The term"heterocyclic ring"or"heterocyclic"or"heterocycle"as used herein, refers to any 3-or 4-membered ring containing a heteroatom selected from oxygen, nitrogen and sulfur ; or a 5-, 6-or 7-membered ring containing one, two, three, or four nitrogen atoms; one oxygen atom; one sulfur atom; one nitrogen atom and one sulfur atom; two nitrogen atoms and one sulfur atom; one nitrogen atom and one oxygen atom; two nitrogen atoms and one oxygen atom; two oxygen atoms in non-adjacent positions; one oxygen atom and one sulfur atom in non-adjacent positions; or two sulfur atoms in non-adjacent positions.

The 5-membered ring has 0-2 double bonds and the 6-and 7-membered rings have 0-3 double bonds. The nitrogen heteroatoms can be optionally quaternized.

The term"heterocyclic"also includes bicyclic groups in which any of the above heterocyclic rings is fused to a benzene ring or a cyclohexane ring or another heterocyclic ring, such as, for example, indolyl, dihydroindolyl, quinolyl, isoquinolyl, tetrahydroquinolyl, tetrahydroisoquinolyl, decahydroquinolyl, decahydroisoquinolyl, benzofuryl, dihydrobenzofuryl or benzothienyl and the like.

Heterocyclic groups include, but are not limited to groups such as, for example, aziridinyl, azetidinyl, epoxide, oxetanyl, thietanyl, pyrrolyl, pyrrolinyl, pyrrolidinyl, pyrazolyl, pyrazolinyl, pyrazolidinyl, imidazolyl, imidazolinyl, <BR> <BR> <BR> imidazolidinyl, pyridyl, tetrahydropyridyl, piperidinyl, homopiperidinyl, pyrazinyl, piperazinyl, pyrimidinyl, pyridazinyl, oxazolyl, oxazolinyl, oxazolidinyl, isoxazolyl, isoxazolidinyl, morpholinyl, thiomorpholinyl, thiazolyl, thiazolinyl, thiazolidinyl, isothiazolyl, isothiazolidinyl, indolyl, quinolinyl, isoquinolinyl, benzimidazolyl, benzothiazolyl, benzoxazolyl, oxetanyl, dihydrofuranyl, tetrahydrofuranyl, dihydropyranyl, tetrahydropyranyl, thienyl, dihydrothienyl, tetrahydrothienyl, triazolyl, triazolinyl, tetrazolyl, tetrazolinyl, isoxazolyl, 1,2,3-oxadiazolyl, 1,2,4- oxadiazolyl, 1,3,4-oxadiazolyl, 1,2,5-oxadiazolyl, oxadiazolinyl,, 1,2,3- thiadiazolyl, 1,2,4-thiadiazolyl, 1,3,4-thiadiazolyl, 1,2,5-thiadiazolyl, thiadiazolinyl, 1,3-dithiolinyl, 1,2-dithiolyl, 1,3-dithiolyl, 1,3-dioxolinyl, didehydrodioxolanyl, 1,3-oxathiolinyl, oxathiolyl, pyrimidyl, benzothienyl and the like. Heterocyclic groups also include compounds of the formula where X* is-CH2 or-O-and Y* is-C (O)- or [-C (R92) 2-] v where R92 is hydrogen or Ci-C4 alkyl where v is 1,2, or 3 such as 1,3-benzodioxolyl, 1,4-benzodioxanyl and the like. Heterocyclic groups also include bicyclic rings such as quinuclidinyl and the like.

Heterocyclic groups can be unsubstituted or substituted with from one to three substituents, each independently selected from loweralkyl, hydroxy, alkoxy, thioalkoxy, amino, alkylamino, dialkylamino and halogen. In addition, nitrogen containing heterocyclic rings can be N-protected.

The term"(heterocyclic) alkenyl"as used herein refers to a heterocyclic group appended to a lower alkenyl radical including, but not limited to, pyrrolidinylethenyl, morpholinylethenyl and the like.

The term" (heterocyclic) alkoxy" as used herein refers to the group having the formula,-OR, where R is a (heterocyclic) alkyl group.

The term" (heterocyclic) alkyl" as used herein refers to a heterocyclic group appended to a loweralkyl radical including, but not limited to, pyrrolidinylmethyl, morpholinylmethyl and the like.

The term" (heterocyclic) alkynyl" as used herein refers to a heterocyclic group appended to a lower alkynyl radical including, but not limited to, pyrrolidinylacetylenyl, morpholinylpropynyl and the like.

The term" (heterocyclic) carbonylalkyl" as used herein refers to a heterocyclic group appended to an alkyl radical via a carbonyl group.

Representative examples of (heterocyclic) carbonylalkyl include pyridylcarbonylmethyl, morpholinocarbonylethyl, piperazinylcarbonylmethyl and the like.

The term" (heterocyclic) carbonyloxyalkyl" as used herein refers to a heterocyclic group appended to an alkyl radical via a carbonyloxy group (i. e., -C (O)-O-). Representative examples of (heterocyclic) carbonylalkyl include pyridylcarbonylmethyl, morpholinocarbonylethyl, piperazinylcarbonylmethyl and the like.

The term" (heterocyclic) oxy" as used herein refers to a heterocyclic group appended to the parent molecular moiety through an oxygen (-O-).

The term"hydroxy protecting group","hydroxyl protecting group"or"-OH protecting group"as used herein refers to refers to groups used to hydroxy groups against undesirable reactions during synthetic procedures. Commonly used hydroxy protecting groups are disclosed in T. H. Greene and P. G. M. Wuts, Protective Groups in Organic Synthesis. 2nd edition, John Wiley & Sons, New York (1991) which is incorporated by reference herein. Such hydroxy protecting groups include : methyl ether; substituted methyl ethers, including, but not limited to, methoxymethyl, methylthiomethyl, t-butylthiomethyl, (phenyldimethylsilyl) methoxymethyl, benzyloxymethyl, p-methoxybenzyloxymethyl, (4-methoxyphenoxy) methyl, t- butoxymethyl, 2-methoxyethoxymethyl, 2,2,2-trichloroethoxymethyl, 2- (trimethylsilyl) ethoxymethyl, tetrahydropyranyl, tetrahydrothiopyranyl, tetrahydrofuranyl, tetrahydrothiofuranyl ether and the like ; substituted ethyl ethers, including, but not limited to, 1-ethoxyethyl, 1- methyl-1-methoxyethyl, 1-methyl-1-benzyloxyethyl,2,2,2-trichloroethyl, trimethylsilylethyl, t-butyl ether and the like ; benzyl ether; substituted benzyl ethers, including, but not limited to, p-methoxybenzyl, 3,4-dimethoxybenzyl, o-nitorbenzyl, p-halobenzyl, p-cyanobenzyl, diphenylmethyl, triphenylmethyl ether and the like ; silyl ethers, including, but not limited to, trimethylsilyl, triethylsilyl, triisopropylsilyl, dimethylisopropylsilyl, diethylisopropylsilyl, dimethylthexylsilyl, t- butyldimethylsilyl,t-butyldiphenylsilyl, tribenzylsilyl, triphenylsilyl, diphenylmethylsilyl ether and the like ; esters, including, but not limited to, formate, acetate, chloroacetate, dichloroacetate, trichloroacetate, trifluoroacetate, methoxyacetate, phenoxyacetate, pivaloate, benzoate ester and the like ; and the like.

Preferred hydroxy protecting groups include substituted methyl ethers, benzyl ether, substituted benzyl ethers, silyl ethers and esters.

The term"hydroxyalkyl"as used herein refers to the group having the formula,-R65-OH, where R65 is an alkylene group The term"leaving group"as used herein refers to a group which is easily displaced from the compound by a nucleophile. Examples of leaving groups include a halide (for example, Cl, Br or 1) or a sulfonate (for example, mesylate, tosylate, triflate and the like) and the like.

The term"N-protecting group"or"N-protected"as used herein refers to those groups intended to protect the N-terminus of an amino acid or peptide or to protect an amino group against undesirable reactions during synthetic procedures. Commonly used N-protecting groups are disclosed in T. H. Greene and P. G. M. Wuts, Protective Groups in Orqanic Synthesis. 2nd edition, John Wiley & Sons, New York (1991). N-protecting groups comprise acyl groups such as formyl, acetyl, propionyl, pivaloyl, t-butylacetyl, 2-chloroacetyl, 2-bromoacetyl, trifluoroacetyl, trichloroacetyl, phthalyl, o-nitrophenoxyacetyl, a-chlorobutyryl, benzol, 4-chlorobenzoyl, 4-bromobenzoyl, 4-nitrobenzoyl, and the like ; sulfonyl groups such as benzenesulfonyl, p-toluenesulfonyl and the like ; sulfenyl groups such as phenylsulfenyl (phenyl-S-), triphenylmethylsulfenyl (trityl-S-) and the like ; sulfinyl groups such as p-methylphenylsulfinyl (p-methylphenyl-S (O)-), t-butylsulfinyl (t-Bu-S (O)-) and the like ; carbamate forming groups such as benzyloxycarbonyl, p-chlorobenzyloxycarbonyl, p-methoxybenzyloxycarbonyl, p-nitrobenzyloxycarbonyl, 2-nitrobenzyloxycarbonyl, p-bromobenzyloxycarbonyl, 3,4-dimethoxybenzyloxycarbonyl, 3,5-dimethoxybenzyloxycarbonyl, 2,4-dimethoxybenzyloxycarbonyl, 4-methoxybenzyloxycarbonyl, 2-nitro-4,5-dimethoxybenzyloxycarbonyl, 3,4,5-trimethoxybenzyloxycarbonyl, <BR> <BR> <BR> <BR> 1- (p-biphenylyl)-1-methylethoxycarbonyl, a, a-dimethyl-3, 5-dimethoxybenzyloxy- carbonyl, benzhydryloxycarbonyl, t-butyloxycarbonyl, diisopropylmethoxy- carbonyl, isopropyloxycarbonyl, ethoxycarbonyl, methoxycarbonyl, allyloxycar- bonyl, 2,2,2-trichloroethoxycarbonyl, phenoxycarbonyl, 4-nitro-phenoxycarbonyl, fluorenyl-9-methoxycarbonyl, cyclopentyloxycarbonyl, adamantyloxycarbonyl, cyclohexyloxycarbonyl, phenylthiocarbonyl and the like ; alkyl groups such as benzyl, p-methoxybenzyl, triphenytmethyl, benzyloxymethyl and the like ; p-methoxyphenyl and the like ; and silyl groups such as trimethylsilyl and the like.

Preferred N-protecting groups include formyl, acetyl, benzol, pivaloyl, t-butylacetyl, phenylsulfonyl, benzyl, t-butyloxycarbonyl (Boc) and benzyloxycar- bonyl (Cbz).

The term"thioalkoxy"as used herein refers to groups having the formula -SR98 wherein R98 is an alkyl group. Preferred groups R98 are loweralkyl groups.

The term"thio-substituted alkyl"as used herein refers to an alkyl radical to which is appended a thiol group (-SH).

As used herein, the terms"S"and"R"configuration are as defined by the IUPAC 1974 Recommendations for Section E, Fundamental Stereochemistry, Pure Appl. Chem. (1976) 45,13-30.

The compounds of the invention can comprise asymmetrically substituted carbon atoms. As a result, all stereoisomers of the compounds of the invention are meant to be included in the invention, including racemic mixtures, mixtures of diastereomers, as well as individual optical isomers, including, enantiomers and single diastereomers of the compounds of the invention substantially free from their enantiomers or other diastereomers. By"substantially free"is meant greater than about 80% free of other enantiomers or diastereomers of the compound, more preferably greater than about 90% free of other enantiomers or diastereomers of the compound, even more preferably greater than about 95% free of other enantiomers or diastereomers of the compound, even more highly preferably greater than about 98% free of other enantiomers or diastereomers of the compound and most preferably greater than about 99% free of other enantiomers or diastereomers of the compound.

In addition, compounds comprising the possible geometric isomers of carbon-carbon double bonds and carbon-nitrogen double are also meant to be included in this invention.

Individual stereoisomers of the compounds of this invention can be prepared by any one of a number of methods which are within the knowledge of one of ordinary skill in the art. These methods include stereospecific synthesis, chromatographic separation of diastereomers, chromatographic solution of enantiomers, conversion of enantiomers in an enantiomeric mixture to diastereomers and then chromatographically separating the diastereomers and regeneration of the individual enantiomers, enzymatic solution and the like.

Stereospecific synthesis involves the use of appropriate chiral starting materials and synthetic reactions which do not cause racemization or inversion of stereochemistry at the chiral centers.

Diastereomeric mixtures of compounds resulting from a synthetic reaction can often be separated by chromatographic techniques which are well-known to those of ordinary skill in the art.

Chromatographic solution of enantiomers can be accomplished on chiral chromatography resins. Chromatography columns containing chiral resins are commercially available. In practice, the racemate is placed in solution and loaded onto the column containing the chiral stationary phase. The enantiomers are then separated by HPLC.

Resolution of enantiomers can also be accomplished by converting the enantiomers in the mixture to diastereomers by reaction with chiral auxiliaries.

The resulting diastereomers can then be separated by column chromatography.

This technique is especially useful when the compounds to be separated contain a carboxyl, amino or hydroxyl group that will form a salt or covalent bond with the chiral auxiliary. Chirally pure amino acids, organic carboxylic acids or organosulfonic acids are especially useful as chiral auxiliaries. Once the diastereomers have been separated by chromatography, the individual enantiomers can be regenerated. Frequently, the chiral auxiliary can be recovered and used again.

Enzymes, such as esterases, phosphatases and lipases, can be useful for solution of derivatives of the enantiomers in an enantiomeric mixture. For example, an ester derivative of a carboxyl group in the compounds to be separated can be prepared. Certain enzymes will selectively hydrolyze only one of the enantiomers in the mixture. Then the resulting ehantiomerically pure acid can be separated from the unhydrolyzed ester.

In addition, solvates and hydrates of the compounds of Formula I, IIA, IIB, IIIA or IIIB are meant to be included in this invention.

When any variable (for example R', R2, R3, m, n, etc.) occurs more than one time in any substituent or in the compound of Formula I, IIA, IIB, IIIA or IIIB or any other formula herein, its definition on each occurrence is independent of its definition at every other occurrence. In addition, combinations of substituents are permissible only if such combinations result in stable compounds. Stable compounds are compounds which can be isolated in a useful degree of purity from a reaction mixture.

This invention is intended to encompass compounds having Formula I, IIA, IIB, IIIA or IIIB when prepared by synthetic processes or by metabolic processes.

Preparation of the compounds of the invention by metabolic processes include those occurring in the human or animal body (in vivo) or processes occurring in vitro.

Compounds of the invention can be prepared according to the methods described in Schemes 1-10 as shown below.

Throughout the schemes, methods will be illustrated wherein R'is a carboxylic acid or carboxylic acid ester substituent. It will be understood by those skilled in the art that other R'substituents can (a) be obtained either from the carboxylic acid or carboxylic acid ester group, (b) can be introduced by similar methods to those used to introduce the carboxylic acid or carboxylic acid ester group or (c) can be introduced by other methods generally known in the art.

In addition, throughout the schemes, methods will be illustrated wherein R4, R6, R7, R8, R9 and R10 are hydrogen. It will be understood by those skilled in the art that compounds wherein one or more of these substituents is other than hydrogen can be prepared by methods analogous to those disclosed in the schemes or by other methods generally known in the art.

In addition, unless otherwise noted, methods will be illustrated for obtaining compounds of the invention having the preferred relative stereochemistry. It will be understood by those skilled in the art that compounds of the invention having other relative stereochemistry can be prepared by methods analogous to those disclosed in the schemes or by other methods generally known in the art.

In addition, throughout the schemes, methods will be illustrated wherein X is-C (=O)-NH-. It will be understood by those skilled in the art that other X groups can be prepared by methods analogous to those disclosed in the schemes or by other methods generally known in the art.

As shown in Scheme 1, reaction of acrolein with an N-protected a-amino acid ester 1 (P'is an N-protecting group, preferably a benzyl group or the like and p2 is a carboxylic acid protecting group, preferably a t-butyl group or the like) in an inert solvent (for example, toluene and the like) in the presence of an acid catalyst (for example, acetic acid and the like), followed by equilibration with a base (for example, with triethylamine or the like) and separation of the isomers by chromatography, provides substituted pyrrolidine 2. Reduction of the aldehyde group to an alcohol with an aldehyde to alcohol reducing agent (for example, sodium borohydride or the like) in an inert solvent (for example, methanol or the like), followed by chromatographic separation of the isomers provides alcohol 3.

Alcohol 3 can be protected with an hydroxy protecting group P3 (preferably with a silyl protecting group, for example, t-butyldimethylsilyl or the like) using standard alcohol protection methods to provide 4. Oxidation of the vinyl group of compound 4 to an aldehyde is accomplished by reacting compound 4 with Os04 and N-methylmorpholine N-oxide to give the corresponding diol. The diol is then treated with sodium periodate to provide aldehyde 5. Substituents R3 can be introduced via reaction of aldehyde 5 with a Grignard reagent (for example, R3MgBr or the like) to give alcohol 6. Oxidation of alcohol 6 (for example, Swern oxidation or the like) provides ketone 7. Reductive amination of ketone 7 (for example, by reaction with ammonium acetate and sodium cyanoborohydride in methanol or the like) gives amine 8. Amine 8 can be further functionalized to complete the introduction of the R2-X-substituent (for example, by reaction of the amine with an acylating agent such as acetic anhydride or the like or by other acylation methods), followed by chromatographic separation of the diastereomers to give 9a. The other diastereomeric amine (9b) can also be isolated and further transformed according to Scheme 1.

Removal of hydroxy protecting group P3 (for example, by reaction with a fluoride ion source, such as tetrabutylammonium fluoride or the like, when P3 is a silyl protecting group) provides alcohol 10. Transformation of the hydroxy group of alcohol 10 allows introduction of various substituents Y.

For example, alkylation of the hydroxy group provides ethers 11. N- deprotection (for example, where P'is a benzyl group, by hydrogenation) gives 12', followed by ester hydrolysis (for example, with acid such as HCI), provides compound 12"of the invention.

Oxidation of the hydroxy group of 10 (for example, Swern oxidation or the like) provides aldehyde 13. Oxidation of aldehyde 13 (for example, with Na102 or the like) provides carboxylic acid 14. The carboxylic acid substituent of 14 can be used to introduce a variety of other functional groups in substituent Y. For example, the carboxylic acid can be esterified (for example, by reaction with diazomethane or with ethanol and DCC or the like) or the carboxylic acid or an activated derivative thereof can be reacted with amines to provide 15 (wherein -C (=O)-R22 represents an ester or an amide). N-deprotection (for example, where P'is a benzyl group, by hydrogenation) gives 16', followed by ester hydrolysis (for example, with acid such as HCI), provides compound 16"of the invention.

Derivatives of the aldehyde group of 13 or the carboxylic acid group of 14 can be used to introduce substituents Y which are-CN or various heterocycles, according to methods known to those skilled in the art and according to the specific methods exemplified herein.

Reaction of aldehyde 13 with loweralkyl-or loweralkenyl-Grignard reagents, followed by oxidation (for example, Swern oxidation or the like), provides ketones 17 wherein R22 is loweralkyl or loweralkenyl. N-deprotection (for example, where P'is a benzyl group, by hydrogenation) gives 18', followed by ester hydrolysis (for example, with acid such as HCI), provides compound 18"of the invention.

Compounds wherein substituent Y is an amino group or a derivative of an amino group can be prepared as shown in Scheme 2. Oxidation of aldehyde 2 (for example, with AgO or NaCIO2 or the like) provides carboxylic acid 19.

Curtius rearrangement of carboxylic acid 19 (for example, reaction with DPPA, Et3N and benzyl alcohol or the like), followed by chromatographic separation of the diastereomers, provides amide 20 wherein P4 is an N-protecting group (for example, benzyloxycarbonyl or the like). Transformations analogous to those which converted compound 4 to compound 9a and 9b in Scheme 1, enable the conversion of 20 to 21a and 21b, which can be separated by chromatography.

Removal of protecting group P4 (for example, by selective hydrogenation) provides 22. Further derivatization of the amino group allows for introduction of substituents Y which are amine derivatives. N-deprotection (for example, where P'is a benzyl group, by hydrogenation), followed by ester hydrolysis (for example, with acid such as HCI), provides compounds of the invention wherein Y is amino or an amine derivative.

Olefination of aldehyde 13 (for example, with Ph3PCH2 or the like), followed by hydrogenation (causing N-deprotection (for example, where P'is a benzyl group) and olefin saturation, followed by ester hydrolysis (for example, with acid such as HCI), provides compounds of the invention wherein Y is loweralkyl.

As shown in Scheme 3, oxidation of the vinyl group of compound 4 to a diol (for example, with Os04 and N-methylmorpholine N-oxide or the like) gives diol 23. Removal of N-protecting group Pr (for example, where P is a benzyl group, by hydrogenation) provides pyrrolidine 24. Reprotection with an acid- labile N-protecting group P5 (for example, t-butoxycarbonyl or the like) provides 25. Transformation of compound 25 to aldehyde 26a and 26b can be accomplished in a manner analogous to conversion of compound 4a to compound 10 and compound 10 to compound 13 as shown in Scheme 1.26a and 26b can be separated by chromatography.

Olefination of 26a (for example, with Ph3PCH2, or triphenylphosine/methylene chloride/n-BuLi, or I-Ph3P+CH2CH3/KOtBu, or the like) provides 27 wherein Y is an olefinic substituent. N-deprotection of the P5 protecting group and ester hydrolysis, under acidic conditions, provides compounds of the invention 28 wherein Y is an olefinic substituent.

In yet another alternative method shown in Scheme 4, the hydroxy group of alcohol 3 is protected with a base-labile hydroxy protecting group p6 (for example, acetyl or the like) to give compound 29. Oxidation of the vinyl group of 29 with Os04 and N-methylmorpholine N-oxide provides diol 30. Removal of the P'protecting group (for example, by hydrogenation or the like) provides pyrrolidine 31. Reprotection with an acid-labile N-protecting group P5 (for example, t-butoxycarbonyl or the like) provides 32. Selective protection of the primary alcohol of 32 with a hydroxy protecting group P7 (for example, a silyl protecting group such as triisopropylsilyl or the like) provides compound 33.

Oxidation of 33 (for example, Swern oxidation or the like) provides ketone 34.

Reductive amination of ketone 34 (for example, by reaction with ammonium acetate and sodium cyanoborohydride in methanol or the like) gives amine 35.

Amine 35 can be further functionalized to complete the introduction of the R 2_X_ substituent (for example, by reaction of the amine with an acylating agent such as acetic anhydride or the like or by other acylation methods), followed by chromatographic separation of the diastereomers to give 36a. The other diastereomeric amine (36b) can also be isolated and further transformed according this scheme.

Selective removal of the p6 hydroxy protecting group in 36a (for example, with K2CO3 in methanol or the like) provides alcohol 37. Oxidation of the alcohol to an aldehyde (for example, Swern oxidation or the like) provides 38. The aldehyde can serve as a precursor for various substituents Y in the compounds of the invention. For example, olefination of 38 (for example, with Ph3PCH2, or triphenylphosine/methylene chloride/n-BuLi, or I-Ph3P+CH2CH3/KOtBu, or the like) provides 39 wherein Y is an olefinic substituent. Removal of the P7 hydroxy protecting group (for example, with a fluoride ion source such as tetrabutylammonium fluoride or the like) gives alcohol 40.

The alcohol can serve as a precursor for a variety of R3 substituents in the compounds of the invention. For example, the alcohol of 40 can be oxidized to an aldehyde (for example, by Dess-Martin oxidation or the like) to give 41.

Aldehyde 41 can be reacted with Grignard reagents (R'4MgBr or the like) or other organometallic reagents (for example, organolithium reagents such as R14Li or the like) to provide 42 as a mixture of alcohol diastereomers which can be separated chromatographically to provide the major isomer 42a and the other isomer 42b. Isomer 42a or the mixture of isomers 42 can be oxidized (for example, by Dess-Martin oxidation or the like) to give ketone 43. Reduction of ketone 43 (for example, with sodium borohydride in ethanol or the like) provides alcohol 42b as the major isomer, which can be isolated by chromatography. N- deprotection of the P5 protecting group and ester hydrolysis, under acidic conditions, provides compounds of the invention 44a or 44b, respectively, wherein Y is an olefinic substituent.

Alkylation of alcohol 42a or 42b provides ethers 45a or 45b, respectively.

N-deprotection of the P5 protecting group and ester hydrolysis, under acidic conditions, provides compounds of the invention 48a or 48b, respectively, wherein Y is an olefinic substituent.

As shown in Scheme 5, reaction of ketone 43 with with Grignard reagents (R37aMgBr or the like) or other organometallic reagents (for example, organolithium reagents such as R37aLi or the like) provides alcools 46a and 46b as a mixture of alcohol diastereomers which can be separated chromatographically. N-deprotection of the P5 protecting group and ester hydrolysis, under acidic conditions, provides compounds of the invention 47a or 47b, respectively, wherein Y is an olefinic substituent.

Alkylation of alcohol 46a or 46b provides ethers 49a or 49b, respectively.

N-deprotection of the P5 protecting group and ester hydrolysis, under acidic conditions, provides-compounds of the invention 50a or 50b, respectively, wherein Y is an olefinic substituent.

Esters or prodrugs of the compounds of the invention can be prepared by methods known in the art.

Scheme 6 illustrates a method for preparing enantiomerically enriched compounds of the invention having the preferred absolute stereochemistry.

Protected pyrrole 51 (wherein P 8 is an N-protecting group, for example, t-butyloxycarbonyl or the like, and P is a hydroxy protecting group, for example, t-butyldimethylsilyl or the like ; J. Org. Chem. 57 3760-3763 (1992)) is reacted with imine 52 (wherein P is an N-protecting group, for example, p-toluenesulfinyl (-S (O) Tol), t-butylsulfinyl (-S (O)-t-Bu), tritylsulfenyl ( (Ph) 3C-S-), phenylsulfenyl (Ph-S-), p-methoxyphenyl, p-methoxybenzyl or the like and wherein any functional groups within group R3 that require protection are appropriately protected) in the presence of a Lewis acid, for example, trimethylsilyltriflate, borontrifluoride etherate or the like, in an inert solvent, for example, dichloromethane or the like, to provide unsaturated lactam 53 Preferably, N-protecting groups P8 and P10 can be selectively deprotected/removed in the presence of each other. Reaction of 53 with an organometallic reagent Y-M (wherein M is a metal), for example, a cuprate reagent or the like, in an inert solvent, for example, THF or the like, provides substituted lactam 54. Lactam 54 is converted to cyano-substituted pyrrolidine 55, for example, by (i) reduction with a lactam reducing agent, for example, diisobutylaluminum hydride or the like, in an inert solvent, for example, THF or the like, followed by (ii) treatment with methanol and a catalytic amount of an acid, for example, pyridinium p-toluenesulfonic acid or the like, followed by (iii) reaction with a cyanide source, for example, trimethylsilylcyanide or the like, in an inert solvent, for example, dichloromethane or the like. Alternatively, lactam 54 is converted to cyano-substituted pyrrolidine 55, for example, by (i) reduction with a lactam reducing agent, for example, diisobutylaluminum hydride or the like, in an inert solvent, for example, THF or the like, followed by (ii) reaction with a cyanide source, for example, trimethylsilylcyanide or the like, in an inert solvent, for example, dichloromethane or the like in the presence of a Lewis acid such as trimethylsilyl triflate or the like. Removal of protecting group P (for example, with an acid such as trifluoroacetic acid, pyridinium p-toluenesulfonic acid or the like in a suitable solvent), followed by reaction of the amine with an acylating agent such as acetic anhydride or the like or by other acylation methods gives 56. Hydrolysis of the nitrile of 56 and removal of protecting group p8, for example, with hydrochloric acid or the like, and deprotection of any protected functional groups within group R3 provides carboxylic acid 57. Esters or prodrugs of 57 can be prepared by methods known in the art.

Among the preferred compounds of the invention are compounds such as 58 or esters or prodrugs thereof wherein R, R and R are as defined most broadly herein. Especially preferred are compounds 58 or esters or prodrugs thereof wherein R37a is loweralkyl or loweralkenyl, R37c is hydrogen, C1-C3 loweralkyl or allyl and R14 is loweralkyl, loweralkenyl or alkoxy- substituted loweralkyl. In those cases where R37c is hydrogen, the hydroxy group will be protected throughout the process of Scheme 6. Compound 58 can be prepared according to the process described in Scheme 6 by first reacting 51 with imine 59.

The N-protected imine 52 is prepared by reaction of the corresponding aldehyde with P 10 NH2.

Scheme 7 illustrates a method for preparing preferred imines 59.

Allylic alcohol 60 (wherein R14a and the carbon to which it is bonded, when taken together, will become substituent R14) is asymmetrically epoxidized, for example, by Sharpless epoxidation with t-butyl hydroperoxide, (-)-dimethyl D- tartrate and titanium tetraisopropoxide or the like in an inert solvent such as dichloromethane and the like and the alcohol is protected (for example, P is benzoate or the like) to give 61. Epoxide 61 is reduced, for example, with lithium aluminum hydride or the like in an inert solvent such as THF or the like, followed by protection of the primary alcohol of 62 (for example, P12 is benzyl or the like) to give 63. Where R 37c is other than hydrogen, 63 is reacted with a non-nucleophilic strong base, for example, sodium bis (trimethylsilyl) amide or the like, and R37C-X wherein X is a halide or other leaving group in an inert solvent such as THF or the like to provide 64. Where R37c is hydrogen, the hydroxy group is protected. Protecting group pl2 is removed, preferably, selectively if any other hydroxy protecting groups are present in the compound, for example, by hydrogenation when it is a benzyl group, and the resulting alcohol is oxidized to an aldehyde, for example, with pyridinium chlorochromate or the like to give aldehyde 65. Reaction of 65 with P1°NH2 gives 59.

An alternative method for preparation of enantiomerically enriched compounds of the invention having the preferred absolute stereochemistry is shown in Scheme 8. Unsaturated lactam 66 (Tetrahedron Asymmetry 1167- 1180 (1996)) wherein p13 iS an N-protecting group, for example, t-butyloxycarbonyl or the like, and P14 is a hydroxy protecting group, for example, t-butyldimethylsilyl or the like, is reacted with an organometallic reagent Y-M (wherein M is a metal), for example, a cuprate reagent or the like, in an inert solvent, for example, THF or the like, to provide 67. Lactam 67 is converted to cyano-substituted pyrrolidine 68, for example, by (i) reduction with a lactam reducing agent, for example, diisobutylaluminum hydride or the like, in an inert solvent, for example, THF or the like, followed by (ii) treatment with methanol and a catalytic amount of an acid, for example, pyridinium p-toluenesulfonic acid or the like, followed by (iii) reaction with a cyanide source, for example, trimethylsilylcyanide or the like, in an inert solvent, for example, dichl. oromethane or the like. Deprotection of the alcohol (for example, with a fluoride ion source such as tetrabutylammonium fluoride or the like when P14 is a silyl based hydroxy protecting group), followed by conversion of the alcohol to an azide group (for example, by reaction with triphenylphosphine, diethyl diazodicarboxylate and diphenylphosphory azide or the like in an inert solvent such as THF or the like) provides 69. The azide is reduced (for example, with triphenylphosphine in THF/water or the like), the resulting amine is acylated (for example, with an acylating agent such as acetic anhydride or the like or by other acylation methods) and the diol is deprotected (for example, with acetic acid or the like) to give 70. Diol 70 is oxidized to the aldehyde 71, for example, with sodium metaperiodate or the like. The aldehyde 71 can be converted to 72 by methods described in Schemes 4 and 5. Compound 72 can be converted to 57 according to the method described in Scheme 6 for converting 56 to 57.

More particularly, compounds 58 wherein R37a is loweralkyl or loweralkenyl, R 37c is hydrogen, Cl-C3 loweralkyl or allyl and R14 is loweralkyl, loweralkenyl or alkoxy-substituted loweralkyl are prepared from 71 according to the methods outlined in Schemes 4 and 5 to give 73, which is converted to 58. In those cases where R 37c is hydrogen, the hydroxy group will be protected throughout the process of Scheme 8.

Another alternative method for preparation of enantiomerically enriched compounds of the invention having the preferred absolute stereochemistry is shown in Scheme 9. Aldehyde 74 (wherein P 15 is an N-protecting group, for example, t-butyloxycarbonyl or the like and, preferably, wherein R37a is loweralkyl or loweralkenyl, R 37c is hydrogen, Cl-C3 loweralkyl or allyl and R14 is loweralkyl, loweralkenyl or alkoxy-substituted loweralkyl) is reacted with N- protected hydroxylamine P16-NHOH wherein P16is an N-protecting group, for example, p-methoxybenzyl or the like, to provide nitrone 75. Preferably, N-protecting groups P15 and p16 can be selectively deprotected/removed in the presence of each other. The carbanion of a carboxy protected propiolate is prepared by reacting the carboxy protected propiolate (P is an acid protecting group, for example, methyl or t-butyl or the like) with a non- nucleophilic strong base, for example, n-BuLi or the like in an inert solvent, for example, THF or the like. The propiolate carbanion is then reacted with nitrone 75 to give 76. Reaction of 76 with zinc dust in acetic acid/methanol provides unsaturated lactam 77. Unsaturated lactam 77 is reacted with an organometallic reagent Y-M (wherein M is a metal), for example, a cuprate reagent or the like, in an inert solvent, for example, THF or the like, to provide 78. Removal of protecting group P15 (for example, with an acid such as trifluoroacetic acid, pyridinium p-toluenesulfonic acid or the like in a suitable solvent), followed by reaction of the amine with an acylating agent such as acetic anhydride or the like or by other acylation methods gives 79.

Optionally, N-protecting group P16 can be replaced by another N-protecting group before completing the process (for example, where P16is p- methoxybenzyl or the like it can be removed and replaced with t-butyloxycarbonyl or the like). Lactam 79 is converted to cyano-substituted pyrrolidine 80, for example, by (i) reduction with a lactam reducing agent, for example, diisobutylaluminum hydride or the like, in an inert solvent, for example, THF or the like, followed by (ii) treatment with methanol and a catalytic amount of an acid, for example, pyridinium p-toluenesulfonic acid or the like, followed by (iii) reaction with a cyanide source, for example, trimethylsilylcyanide or the like, in an inert solvent, for example, dichloromethane or the like. Hydrolysis of the nitrile of 80 and removal of protecting group p16, for example, with hydrochloric acid or the like, provides carboxylic acid 58. In those cases where R 37c is hydrogen, the hydroxy group will be protected throughout the process of Scheme 9. Esters or prodrugs of 58 can be prepared by methods known in the art.

Compound 74 can be prepared according to the process shown in Scheme 10. In Scheme 10, P15is exemplified by t-butyloxycarbonyl (Boc), but can be other N-protecting groups. Compound 81 is prepared from D-serine according to Campbell, et al., Synthesis 1707 (1998). Reaction of 81 with an organometallic reagent R14-M wherein M is a metal (for example, a Grignard reagent (R14-MgCI or R14-MgBr or the like) in an inert solvent, for example, THF or the like, provides 82. Ketone 82 is reacted with an organometallic reagent R37a-M wherein M is a metal (for example, a Grignard reagent, R37a-MgCl or R37a -MgBr or the like) in an inert solvent, for example, THF or the like, to provide 83. Where R37c is other than hydrogen, 83 is reacted with a non-nucleophilic strong base (for example, sodium hydride or the like) in an inert solvent such as THF or the like, followed by reaction with R37c-X where X is a leaving group such as a halide or the like to provide 84.

Where R37c is hydrogen, the hydroxy group is suitably protected.

Deprotection of 84, for example, with p-toluenesulfonic acid in methanol or the like, provides 85. Oxidation of 85, for example, with pyridine sulfur trioxide complex, DMSO and pyridine or the like, gives aldehyde 86, which corresponds to 74 wherein p15 iS t-butyloxycarbonyl.

SCHEME 1 SCHEME 1 (cont'd.) R22C(O)- is an ester or amide SCHEME 1 (cont'd.) R22 is loweralkyl or loweralkenyl SCHEME 2 SCHEME 3 27 28 Y is an alkene SCHEME 4 31 32 SCHEME 4 (cont'd.) 39 Y is an alkene 40 or haloalkene SCHEME 4 (cont'd.) 48a 48b SCHEME 4 (cont'd.) SCHEME 5 SCHEME 5 (cont'd.) SCHEME 6 SCHEME 7 SCHEME 8 SCHEME 8 (cont'd.) SCHEME 9 SCHEME 10 The other compounds of the invention can be readily prepared from the compounds described herein using techniques known in the chemical literature.

The methods required are known and can be readily practiced by those having ordinary skill in the art.

Key intermediates for the preparation of compounds of the invention include the following : (1) wherein P'is an N-protecting group (preferably, a benzyl group or a substituted benzyl group) and p2 is a carboxylic acid protecting group (preferrably, a loweralkyl group, especially t-butyl) ; preferrably, Pr and p2 can be selectively deprotected/removed; or a salt thereof; (2) wherein P'is an N-protecting group (preferably, a benzyl group or a substituted benzyl group) and p2 is a carboxylic acid protecting group (preferably, a loweralkyl group, especially t-butyl) ; and P3 is hydrogen or a hydroxy protecting group (preferably, an acyl protecting group, for example, acetyl and the like, or a silyl protecting group, for example, t-butyldimethylsilyl and the like) ; preferrably, p1, p2 and P3 can be selectively deprotected/removed ; or a salt thereof; (3) wherein P1 is an N-protecting group (preferably, a benzyl group or a substituted benzyl group) and p2 is a carboxylic acid protecting group (preferably, a loweralkyl group, especially t-butyl) ; and P4 is hydrogen or an N-protecting group (preferably, a carbamate N-protecting group, for example, benzyloxycarbonyl and the like) ; preferrably, P', p2 and P4 can be selectively deprotected/removed; or a salt thereof; (4) or wherein p5 is an N-protecting group (preferably, an acid labile N-protecting group, such as t-butyloxycarbonyl and the like) and p2 is a carboxylic acid protecting group (preferably, a loweralkyl group, especially t-butyl) ; and P6 is hydrogen or a hydroxy protecting group (preferably, a base labile hydroxy protecting group, such as acetyl and the like) ; and P7 is hydroxy protecting group (preferably, a silyl protecting group, such as triisopropylsilyl and the like) ; preferrably, p2, p5, p6 and P7 can be selectively deprotected/removed ; or a salt thereof; and (5) or wherein P5 is an N-protecting group (preferably, an acid labile N-protecting group, such as t-butyloxycarbonyl and the like) and p2 is a carboxylic acid protecting group (preferably, a loweralkyl group, especially t-butyl) ; and p6 is hydrogen or a hydroxy protecting group (preferably, a base labile hydroxy protecting group, such as acetyl and the like) ; and P7 iS hydroxy protecting group (preferably, a silyl protecting group, such as triisopropylsilyl and the like) ; and R2 is defined as herein (preferably, loweralkyl or haloloweralkyl ; most preferably, methyl or trifluoromethyl) ; preferrably, p2, p5, p6 and P7 can be selectively deprotected/removed; or a salt thereof.

Other key intermediates for the preparation of compounds of the invention include compounds, including mixtures of compounds having the indicated relative stereochemistry or enantiomerically enriched compounds having the indicated absolute stereochemistry, of the formula (6)- (17).

(6) PLOHN \ "nez ,-X 8 p/8 ° wherein P8is an N-protecting group (including, t-butyloxycarbonyl or the like), p10 iS an N-protecting group (including, p-toluenesulfinyl (-S (O) Tol), t-butylsulfinyl (-S (O)-t-Bu), tritylsulfenyl ((Ph) 3C-S-), phenylsulfenyl (Ph-S-), p-methoxyphenyl, p-methoxybenzyl or the like) and R3 is defined as above (both in terms of its broadest. definition and in-terms of each of the preferred embodiments) ; or a salt thereof. In addition, any functional groups in substituent R3 can be suitably protected. Most highly preferred is substituent R3 of the formula : \ R14 1-'-== R R37a wherein R37a, R37C and R'4 are as defined above (both in terms of their broadest definitions and in terms of each of their preferred embodiments). Especially preferred are compounds wherein R3'a is loweralkyl or loweralkenyl, R"c is hydrogen, C,-C3 loweralkyl or allyl and R'4 is loweralkyl, loweralkenyl or alkoxy- substituted loweralkyl. In those cases where R37C is hydrogen, the hydroxy group can be protected with a hydroxy protecting group. Preferably, p8 and P10 can be selectively deprotected/removed. wherein P8, Plo and R3 are defined as in (6) above and wherein Y is defined as above (both in terms of its broadest definition and in terms of each of the preferred embodiments); or a salt thereof. wherein p8, p10, R3 and Y are defined as in (7) above; or a salt thereof.

(9) wherein p8, R3 and Y are defined as in (7) above and R2 is defined as above (both in terms of its broadest definition and in terms of each of the preferred embodiments); or a salt thereof.

(10) p10 N OR R R14 R37a wherein P°, R14, R37a and R37C are as defined in (6) above; or a salt thereof.

(11) wherein R14, R37a and R37c are as defined in (6) above; or a salt thereof.

(12) y R (0) CHN s 37c/'C N OR P13 P wherein R14 R37a and R37C are as defined in (6) above, R 2 is as defined in (9) above and p13 is an N-protecting group (including, t-butyloxycarbonyl or the like) ; or a salt thereof.

(13) wherein R14, R37a and R37c are as defined in (6) above and p15 is an N-protecting group (including, t-butyloxycarbonyl or the like) ; or a salt thereof.

(14) wherein R14, R37a, R37C and p15 are as defined in (13) above and p16 is an N-protecting group (including, p-methoxybenzyl or the like) ; or a salt thereof.

Preferably, pl5 and p16 can be selectively deprotected/removed.

(15) wherein R14, R37a, R37c, P15 and p16 are as defined in (14) above and p17 is an acid protecting group (including, methyl or t-butyl or the like) ; or a salt thereof.

(16) wherein R14, R37a, R37c and P16 are as defined in (14) above and R2 is as defined in (9) above ; or a salt thereof.

(17) wherein R14, R37a, R37c, P16 and R2 are as defined in (16) above; or a salt thereof.

The reagents required for the synthesis of the compounds of the invention are readily available from a number of commercial sources such as Aldrich Chemical Co. (Milwaukee, WI, USA); Sigma Chemical Co. (St. Louis, MO, USA); and Fluka Chemical Corp. (Ronkonkoma, NY, USA); Alfa Aesar (Ward Hill, MA 01835-9953); Eastman Chemical Company (Rochester, New York 14652-3512); Lancaster Synthesis Inc. (Windham, NH 03087-9977); Spectrum Chemical Manufacturing Corp. (Janssen Chemical) (New Brunswick, NJ 08901); Pfaltz and Bauer (Waterbury, CT. 06708). Compounds which are not commercially available can be prepared by employing known methods from the chemical literature.

The following examples will serve to further illustrate the preparation of the compounds of the invention, without limitation.

Example1 <BR> <BR> <BR> ()- (2R. 3R, 5R. 1'S)-(1-Acetamido-3-ethyl) pentvl-3- (methoxymethyl)-pyrrolidine- 5-carboxylic Acid Hydrochloride.

1A. ()- (2S. 3R. 5R)- and ft)- (2S. 3S. 5R)-1-Benzyl-2-vinyl-3-formyl-pyrrolidine- 5-carboxylic Acid t-Butyl Ester (8 : 1 ratio).

Acrolein (8 mL, 120 mmole) was added to a solution of t-butyl N-benzyl- glycinate (4.34 g, 19.6 mmole) and acetic acid (5 drops) in toluene (100 mL). The solution was heated at reflux. After 1 hour, the reaction was cooled to about 50 °C and an additional 3 mL of acrolein were added. The reaction was heated at reflux for an additional 2 hours and concentrated in vacuo. The residue was purified by chromatography on silica gel using 5% ethyl acetate/hexanes to provide a mixture of ()- (2S, 3R, 5R)- and ()- (2S, 3S, 5R)-1-benzyl-2-vinyl-3- formyl-pyrrolidine-5-carboxylic acid t-butyl esters as an oil (yield : 2.78 g, 45%).

The mixture of aldehydes was equilibrated to an 8: 1 ratio by stirring the crude product with triethylamine (0.5 mL) in ethyl acetate at room temperature followed by evaporation of the solvents.

1 H NMR (CDC13) (major isomer only) : 51. 45 (s, 9H), 2.26 (m, 1 H), 2.69 (m, 1 H), 3.49 (dd, J=7.8,3.0 Hz, 1H), 3.61 (d, J=13. 5 Hz, 1H), 3.93 (m, 1H), 3.94 (d, J=13. 5 Hz, 1H), 5.22-5.33 (two dd, 2H), 5.7 (ddd, J=17. 7, 10.2,7.8 Hz, 1H), 7.21-7.35 (m, 5H), 9.71 (d, J=1.2 Hz, 1H).

MS (M+H) + = 316.

1 B. (i !-(2S. 3R5R)-1-Benzvl-2-vinvl-3-(hydroxymethvl)-pyrrolidine-5-carbo xylic<BR> <BR> <BR> <BR> Acid t-Butyl Ester.

A solution of the 8: 1 mixture of ()- (2S, 3R, 5R)- and ()- (2S, 3S, 5R)-1- benzyl-2-vinyl-3-formylpyrrolidine-5-carboxylic acid t-butyl ester (6.0 g, 19.0 mmole), prepared according to the method described in Example 1A, in 100 mL of methanol was cooled to 0 °C and treated with sodium borohydride (0.72 g, 19.0 mmole). The mixture was stirred for 0.5 hour, warmed to room temperature, and stirred for an additional 1 hour. The reaction was quenched with aqueous ammonium chloride, and the solvent was evaporated. The residue was parti- tioned between ethyl acetate and water. The organic layer was dried over MgS04, filtered and concentrated in vacuo. The residual oil was purified by chromatography on silica gel using a gradient of 20-30% ethyl acetate/hexanes to furnish the title compound as a colorless oil (yield : 4.0 g, 66%).

1 H NMR (CDC13) : 51. 46 (s, 9H), 1.80 (m, 1H), 2.16 (m, 1H), 2.39 (m, 1H), 2.54 (m, 1H), 3.48-3.53 (m, 2H), 3.08 (d, 2H), 3.91 (d, 2H), 5.17-5.22 (m, 2H), 5.70 (m, 1 H), 7.23-7.34 (m, 5H).

MS (M+H) + = 318.

1C. (~)-(2S, 3R. 5R)-1-Benzvl-2-vinyl-3-(t-butYldimethylsilyloxYmethyl)- nyrrolidine-5-carboxylic Acid t-Butyl Ester.

A solution of ()- (2S, 3R, 5R)-1-benzyl-2-vinyl-3-(hydroxymethyl)- pyrrolidine-5-carboxylic acid t-butyl ester (3.6 g, 11.4 mmole), tert-butyidimethylsilyl chloride (3.7 g, 24.5 mmole) and imidazole (2.8 g, 41.2 mmole) in 80 mL of DMF was stirred at room temperature for 1.5 hours. The reaction was diluted with ethyl acetate, washed with water and brine, dried over MgS04, and concentrated in vacuo. The residue was purified by chromatography on silica gel using 5% ethyl acetate/hexanes to provide the title compound, as a colorless oil (yield : 3.5 g, 71%).

1H NMR (CDC13) : 80. 02 (d, 6H), 0.86 (s, 9H), 1.43 (s, 9H), 1.67 (ddd, 1H), 2.11 (m, 1H), 2.28 (m, 1H), 3.40-3.70 (m, 6H), 3.90 (d, 2H), 5.11-5.19 (m, 2H), 5.69 (ddd, 1H), 7.20-7.30 (m, 5H).

MS (M+H) + = 432.

1 D. ()- (2R, 3R, 5R)-1-Benzyl-2-formvl-3- (t-butyldimethvlsilyloxymethyl pyrrolidine-5-carboxylic Acid t-Butyl Ester.

Osmium tetroxide (20 mg) was added to a room temperature solution of ()- (2S, 3R, 5R)-1-benzyl-2-vinyl-3- (t-butyldimethylsilyloxymethyl)-pyrrolidine-5- carboxylic acid t-butyl ester (3.5 g, 8.12 mmole) in 60 mL of 8: 1 acetone/water and N-methylmorpholine N-oxide (3.0 g, 25.6 mmote). The reaction mixture was stirred at room temperature for 6 hours and quenched with saturated aqueous Na2S203. The mixture was stirred for an additional 10 minutes and the solvent removed. The brownish residue was partitioned between dichloromethane and water. The organic layer was dried over MgS04 and concentrated in vacuo to provide the intermediate diol as an oil (-3. 8g) which was used without additional purification.

MS (crude): (M+H) + = 466 The crude diol was dissolved in 6: 1 tetrahydrofuran (THF)/water (50 mL) and treated with sodium periodate (3.0 g, 14.0 mmole). The mixture was stirred at room temperature for 1 hour and diluted with ethyl acetate, washed with water, dried over MgS04, filtered, and concentrated in vacuo. The crude aldehyde was purified by chromatography on silica gel using 3% ethyl acetate/hexanes to provide the title compound as a colorless oil (yield : 1.6 g, 46%).

1 H NMR (CDC13) : 80. 03 (d, 6H), 0.86 (s, 9H), 1.46 (s, 9H), 1.72 (m, 1 H), 2.26-2.45 (m, 2H), 3.53-3.71 (m, 5H), 3.84 (d, 1H), 3.93 (d, 1 H), 7.27-7.31 (m, 5H), 9.32 (d, 1 H).

MS (M+H) + = 434.

1 E. t)-f2R, 3R. 5R)-1-Benzyl-2- (1-oxo-3-ethvl) pentyl-3- (t-butyldimethvlsilyloxv-<BR> <BR> <BR> <BR> <BR> <BR> methypvrrolidine-5-carboxilic Acid t-Butyl Ester.

A dry flask containing magnesium (0.14 g, 5.83 mmole), under argon, was charged with 10 mL of dry THF and 3 drops of dibromoethane. This was followed by addition of 1-bromo-2-ethylbutane (0.95 g, 5.83 mmole). The reaction mixture was heated at reflux for 45 minutes, until most of the magnesium had reacted.

The reaction mixture was cooled to-30 °C and ()- (2R, 3R, 5R)-1-benzyl-2-formyl- 3- (t-butyldimethylsilyloxymethyl)-pyrrolidine-5-carboxylic acid t-butyl ester (0.5 g, 1.15 mmole) in of THF (6 mL) was added, dropwise. The reaction was slowly warmed to-10 °C, over a period of about 2 hours,. and quenched with aqueous ammonium chloride. The resultant slurry was diluted with ethyl acetate and washed with water, brine, and dried over MgS04 and concentrated. The crude alcohol product, an oil (-0. 85 g), was used without further purification.

MS (M+H) + = 520.

A solution of oxalyl chloride (2.5 mL, 2M in CH2CI2) in 10 mL of anhydrous dichloromethane was prepared and maintained under a nitrogen atmosphere, at -78 °C. DMSO (0.77 mL, 9.83 mmole) was added slowly to the solution. The mixture was stirred for 15 minutes and treated with the crude alcohol prepared above, about 0.85 g,-in-5 mL of anhydrous dichloromethane. The solution was stirred for 1 hour and triethylamine (2.3 mL, 16.4 mmole) was added slowly to the reaction mixture. The solution was then allowed to slowly warmed to room temperature and diluted with dichloromethane. The organic layer was washed with water, dried over MgS04, and concentrated. The residue was purified by chromatography on silica gel using 3% ethyl acetate/hexanes to provide the title compound, as an oil (yield : 0.35 g, 66%).

MS (M+H) + = 518.

1F. (~)-(2R,3R,5R,1'R)- and (~)-(2R,3R,5R,1'S)-1-Benzyl-2-(1-amino-3-ethyl)- <BR> <BR> <BR> pentyt-butyldimethylsilyloxymethyl)-pyrrolidine-5-carboxylic Acid t-Butyl Ester.

A solution of (+)-(2R, 3R, 5R)-1-benzyl-2-(1-oxo-3-ethyl) pentyl-3-(t-butyl- dimethylsilyloxymethyl)-pyrrolidine-5-carboxylic acid t-butyl ester (0.20 g, 0.39 mmole), ammonium acetate (30 equiv.) and sodium cyanoborohydride (10 equiv.) in 5 mL of methanol was heated at reflux for 24 hours with occasional addition of an additional 60 equivalents of ammonium acetate and 20 equivalents of sodium cyanoborohydride. The solvent was evaporated. The resultant residue was partitioned between dichloromethane and water. The organic layer was dried over MgS04, filtered, and concentrated. The product was purified by chromatography on silica gel using 30-50% ethyl acetate/hexanes to provide _ title compound as a colorless oil (yield : 130 mg, 64%).

'H NMR (CDC13) 5 7.30 (m, 5H), 4.91 (s, 1H), 3.53 (m, 2H), 3.08 (m, 1H), 2.88 (m, 1 H), 2.35 (m, 1 H), 1.85 (m, 1 H), 1.44 (s, 9H), 1.20-1.40 (m, 7H), 0.88 (s, 9H), 0. 85 (m, 6H), 0.03 (s, 6H) MS (M+H) + = 519.

1G.(iW2R.3R.5R.1'S)-1-Benzvt-2-(1-acetamido-3-ethv))Dentv)-3 --butv!- dimethvisilyloxymethyl)-pyrrolidine-5-carboxylic Acid t-Butvl Ester.

A solution of ()- (2R, 3R, 5R, 1'R)- and ()- (2R, 3R, 5R, 1'S)-1-benzyl-2- (1- amino-3-ethyl) pentyl-3- (t-butyldimethylsilyloxymethyl)-pyrrolidine-5-carboxylic acid t-butyl ester (110 mg, 0.21 mmole) and acetic anhydride (214 mg, 2.1 mmole) in 10 mL of dichloromethane was stirred for 1 hour. The solvent and excess acetic anhydride were removed in vacuo. The residue was purified by chromatography on silica gel using 30% ethyl acetate/hexanes to provide the title compound as a white solid (yield : 85 mg, 72%).

'H NMR (CDC13) â 7.28 (m, 5H), 5.14 (d, J=14Hz, 1H), 4.36 (m, 1H), 3.95 (m, 2H), 3.62 (m, 1 H), 3.52 (m, 1 H), 3.45 (m, 1 H), 2.98 (m, 1 H), 1.98 (s, 3H), 1.60 (m, 2H), 1.43 (s, 9H), 1.20-1.40 (m, 7H), 0.88 (s, 9H), 0.80 (m, 6H), 0.04 (s, 6H) MS (M+H) + = 561.

1H.()-(2R.3R.5R.1'S)-1-Benzvt-2-f1-acetamido-3-ethvt)Dentv!- 3-hvdroxv- methyl)-nyrrolidine-5-carboxylic Acid t-Butyl Ester.

A solution of ()- (2R, 3R, 5R, 1'S)-1-benzyl-2- (1-amino-3-ethyl) pentyl-3- (t- butyldimethylsilyloxymethyl)-pyrrolidine-5-carboxylic acid t-butyl ester (85 mg, 0.15 mmole) in dry THF (5 mL) was prepared and maintained at room tempera- ture under a nitrogen atmosphere. Tetrabutylammonium fluoride (1 M in THF, 0.23 mL) was added slowly to the solution. The reaction mixture was stirred for 1 hour. The solvent was removed in vacuo and the residue purified by chromatography on silica gel using 30-50% ethyl acetate/hexanes to provide the title compound as a white foam (yield : 41 mg, 61 %).

'H NMR (CDCl3) 8 7.20-7.35 (m, 5H), 5.20 (d, J=14Hz, 1H), 4.28 (m 1H), 4.93 (m, 2H), 3.65 (m, 2H), 3.50 (m, 1 H), 3.23 (m, 2H), 2.22 (m, 2H), 1.98 (s, 3H), 1.62 (m, 1H), 1.43 (s, 9H), 1.15-1.40 (m, 7H), 0.80 (m, 6H) MS (M+H) + = 447.

11. (i)-(2R, 3 R. 5R. 1'S)-1-Benzyl-2-(1-acetamido-3-ethYl) pentvl-3-(methoxy- methvl)-nyrrolidine-5-carboxylic Acid t-Butyl Ester.

A mixture of ()- (2R, 3R, 5R, 1'S)-1-benzyl-2- (1-acetamido-3-ethyl) pentyl-3- (hydroxymethyl)-pyrrolidine-5-carboxylic acid t-butyl ester (40 mg, 0.09 mmole) and silver oxide (200 mg, 0.90 mmole) in 3 mL of iodomethane was heated at reflux for three hours. The reaction was cooled, filtered, and the solvent was removed in vacuo, to provide the title product as a crude oil.

MS (M+H) + = 461.

1J. ()- (2R, 3R. 5R, 1'S)-2- (1-Acetamido-3-ethyl) pentyl-3- (methoxymethyl-<BR> <BR> <BR> pyrrolidine-5-carboxylic Acid t-Butyl Ester.

A mixture of the crude ()- (2R, 3R, 5R, 1'S)-1-benzyl-2-(1-acetamido-3- ethyl) pentyl-3- (methoxymethyl-pyrrolidine-5-carboxylic acid t-butyl ester (32 mg, 0.07 mmole), prepared according to the method described in Example 11, and ammonium formate (130 mg, 2.1 mmole) in ethanol (5 mL) was heated at reflux in the presence of a catalytic amount of 10% palladium, on activated carbon, for 1.5 hours. The reaction was filtered and concentrated in vacuo. The residue was purified by chromatography on silica gel using 50% ethyl acetate/hexanes followed by 10% methanol/dichloromethane to provide the title compound as a colorless oil (yield : 16 mg, 47%).

MS (M+H) + = 371.

1 K. (i)-(2R. 3R, 5R.-1'S)-2-(i-Acetamido-3-ethyl) pentyl-3-(methoxymethyl)- pyrrolidine-5-carboxylic Acid Hydrochloride.

A solution of the ()- (2R, 3R, 5R, 1'S)-2-(1-acetamido-3-ethyl)pentyl-3- (methoxymethyl)-pyrrolidine-5-carboxylic acid t-butyl ester (15 mg) was dissolved in 6 N HCI in water (1 mL) and stirred at room temperature for 3 hours. The solvent was removed under high vacuum to provide the title compound as a white solid.

'H NMR (d6-DMSO) 5 8.10 (d, J=14Hz, 1H), 4.28 (m, 1H), 4.18 (m, 1H), 3.45 (m, 1 H), 3.22 (s, 3H), 2.47 (m, 1 H), 2.38 (m, 1 H), 1.90 (m, 1 H), 1.88 (s, 3H), 1.15-1.42 (m, 7H), 0.82 (t, J=12.5Hz, 3H), 0.79 (t, J=12.5Hz, 3H) MS (M+H) + = 315, (M-H)-= 313.

Example 2 <BR> <BR> <BR> (i)-(2 R. 3R. 5R, 1'S)-2-(1-Acetamido-3-ethyl) pentyl-3-methoxycarbonyl-pyrrolidine- 5-carboxylic Acid Hydrochloride.

2A. ()- (2R, 3R, 5R, 1'S)-1-Benzyl-2- (1-acetamido-3-ethy ) pentvl-3-formyl- pyrrolidine-5-carboxylic Acid t-Butyl Ester.

A solution of oxalyl chloride (0.11 mL, 2M in CH2CI2) in 5 mL of anhydrous dichloromethane was prepared and maintained, under a nitrogen atmosphere, at -78 °C. DMSO (32 mg, 0.42 mmole) was added slowly to the solution. The mixture was stirred for 15 minutes and treated with ()- (2R, 3R, 5R, 1'S)-1-benzyl- <BR> <BR> 2- (1-acetamido-3-ethyl) pentyl-3-hydroxymethyl-pyrrolidine-5-carboxylic acid t- butyl ester (38 mg, 0.085 mmole) in 5 mL of dichloromethane. The solution was stirred for 1 hour and triethylamine (86 mg, 0.85 mmole) was added slowly to the reaction mixture. The solution was allowed to warm to room temperature and diluted with dichloromethane. The organic layer was washed with water and brine, dried over MgS04, filtered and concentrated. The residue was purified by chromatography on silica gel using 3% ethyl acetate/hexanes to provide the title compound as a colorless oil (yield : 39 mg, 97%).

'H NMR (CDC13) 8 9.68 (d, J=1. OHz, 1H), 7.28 (m, 5H), 5.06 (d, J=14Hz, 1H), 4.38 (m, 1H), 4.10 (m, 1H), 3.75 (m, 2H), 3.45 (m, 1H), 2.62 (m, 1H), 2.20 (m, 2H), 1.98 (s, 3H), 1.42 (s, 9H), 1.25-1.40 (m, 7H), 0.82 (m, 6H) MS (M+H) + = 445.

2B. (i)-(2R, 3R. 5R, 1'S)-1-Benzyl-2-(1-acetamido-3-ethyl)pentyl-3-carboxyl- pyrrolidine-5-carboxylic Acid t-Butyl Ester.

A solution of Na102 (0.16 g) and NaH2PO4. H20 (0.17g) in water (1 mL) was added to a solution of ()- (2R, 3R, 5R, 1'S)-1-benzyl-2-(1-acetamido-3- ethyl) pentyl-3-formyl-pyrrolidine-5-carboxylic acid t-butyl ester (35 mg, 0.079 mmole) and 2-methyl-2-butene (0.5 mL) dissolved in t-BuOH (1.5 mL) and acetonitrile (1.5 mL) at 0 °C. After 1 hour the reaction was quenched with 10% aqueous Na2S203 and extracted with dichloromethane. The organic layer was washed with water and brine, dried (MgS04) and concentrated to provide the title product (yield : ~30 mg).

MS (M+H) + = 461.

2C. (i)-(2R, 3R. 5R. 1'S)-1-Benzyl-2-(1-acetamido-3-ethyl) pentyl-3- methoxycarbonyl-pyrrolidine-5-carboxylic Acid t-Butyl Ester.

A solution of Diazaid Tll (0.5 g, 2.33 mmole) in 5 mL of ether was added slowly to a solution of aqueous KOH (0.5 g in 1 mL of water) and 1 mL of ethanol maintained at 65 °C. Diazomethane was distilled into a receiving flask charged with a solution of ()- (2R, 3R, 5R, 1'S)-1-benzyl-2-(1-acetamido-3-ethyl)pentyl-3- carboxyl-pyrrolidine-5-carboxylic acid t-butyl ester (30 mg, 0.065 mmole) in 3 mL of THF. The receiving flask was cooled to 0 °C in an ice/water bath. The condenser was cooled with dry ice/acetone and 3 mL of ether was added the distilling flask until the distillate was colorless. The reaction was stirred for an additional 0.5 hours at 0 °C. The yellowish reaction mixture was quenched with acetic acid (0.1 mL) and diluted with ethyl acetate. The organic layer was washed with 10% NaHCO3 and brine, dried with MgS04 and concentrated in vacuo. The residue was purified by chromatography on silica gel using 50 % ethyl acetate/hexanes to provide the title compound as a colorless oil (yield : 20 mg, 65%).

1H NMR (CDC13) 8 7.25 (m, 5H), 5.10 (d, J=14Hz, 1H), 4.23 (m, 1H), 4.08 (m, 1 H), 3.85 (m, 1H), 3.72 (m, 1 H), 3.69 (s, 3H), 3.40 (m, 1H), 2.75 (m, 1H), 2.33 (m, 1H), 2.15 (m, 1 H), 1. 98 (s, 3H), 1.42 (s, 9H), 1.20-1.40 (m, 7H), 0.83 (m, 6H) MS (M+H) + = 475.

2D. (i)-(2R. 3R. 5R. 1'S)-2-(1-Acetamido-3-ethyl) pentyl-3-methoxycarbonvl- pvrrolidine-5-carboxylic Acid t-Butyl Ester.

A mixture of ()- (2R, 3R, 5R, 1'S)-1-benzyl-2-(1-acetamido-3-ehtyl)pentyl-3- methoxycarbonyl-pyrrolidine-5-carboxylic acid t-butyl ester (14 mg, 0.03 mmole) and ammonium formate (0.3 g) in ethanol (1.5 mL) with a catalytic amount of 10% palladium on activated carbon was heated at about 75 °C, for 1 hour. After filtration to remove the catalyst, the solvent was removed in vacuo. The residue was purified by chromatography on silica gel to provide the title compound as a colorless oil (yield : 8.5 mg, 73%).

MS (M+H) + = 385.

2E. (t)- (2R. 3R, 5R. 1'S)-2- (1-Acetamido-3-ethyl) pentrl-3-methoxycarbonyl- pyrrolidine-5-carboxylic Acid Hydrochloride.

A solution of ()- (2R, 3R, 5R, 1'S)-2-(1-acetamido-3-ethyl)pentyl-3-methoxy- carbonylpyrrolidine-5-carboxylic acid t-butyl ester (8.5 mg, 0.022 mmole) in 4 N HCI in dioxane (1 mL) was stirred at room temperature for 24 hours. The solvent was removed in vacuo to provide the title compound as an off-white solid (yield 8 mg, 100%).

1H NMR (d6-DMSO) 8 8.02 (d, J=14Hz, 1H), 4.40 (m, 1H), 4.22 (m, 1H), 3.85 (t, J=13Hz, 1H), 3.70 (m, 1 H), 3.65 (s, 3H), 3.15 (m, 1H), 2.55 (m, 1H), 2.20 (m, 1H), 1.84 (s, 3H), 1.12-1.42 (m, 7H), 0.82 (t, J=12.5Hz, 3H), 0.68 (t, 3H) MS (M+H) + = 329, (M-H)-= 327.

Example 3 (t)- (2R. 3R. 5R, 1'S)-2- 1-Acetamido-3-ethyl) Penyl-3-cyano-pyrrolidine-5- carboxylic Acid Hydrochloride.

3A. ()- (2R, 3R, 5R, l'S)-l-Benzyl-2- (l-acetamido-3-ethvl) penlyl-3- (hydroxyiminformyl)-pyrrolidine-5-carboxylic Acid t-Butyl Ester.

The title compound is prepared by reacting a solution of ()- (2R, 3R, 5R, 1'S)-1-benzyl-2-(1-acetamido-3-ethyl)pentyl-3-formyl-pyrroli dine-5- carboxylic acid t-butyl ester with hydroxylamine hydrochloride and 10% aqueous potassium carbonate in methanol according to the procedure described by Chelucci et a/., Tetrahedron : Asymmetry 5 : 1973 (1994).

3B. (~)-(2R,3R,5R,1'S)-1-Benzyl-2-(1-acetamido-3-ethyl)pentyl-3- cyano- pyrrolidine-5-carboxylic Acid t-Butyl Ester.

The title compound is prepared by reacting a solution of ()- (2R, 3R, 5R, 1'S)-1-benzyl-2-(1-acetamido-3-ethyl)pentyl-3-(hydroxyiminof ormyl)- pyrrolidine-5-carboxylic acid t-butyl ester with 1, 1'-acrbonyldiimidazole in dichloromethane according to the procedure described by Chelucci et a/., Tetrahedron : Asymmetry 5: 1973 (1994). carboxylic Acid t-Butyl Ester.

The title compound is prepared according to the method described in Example 1J, substituting (i)-(2R, 3R, 5R, 1'S)-1-benzyl-2-(1-acetamido-3- ethyl) pentyl-3-cyano-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3R, 5R, 1'S)-1-benzyl-2-(1-acetamido-3-ethyl)pentyl-3-methoxymethyl- pyrrolidine-5-carboxylic acid t-butyl ester.

3D. (~)-(2R,3R,5R,1'S)-2-(1-Acetamido-3-ethyl)pentyl-3-cyano-pyr rolidine-5- carboxylic Acid Hydrochloride.

The title compound is prepared according to the method described in Example 1K, substituting ()- (2R, 3R, 5R, 1'S)-2-(1-acetamido-3-ethyl)pentyl-3- cyano-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3R, 5R, 1'S)-2- (1-acetamido-3-ethyl) pentyl-3- (methoxymethyl)-pyrrolidine-5-carboxylic acid t- butyl ester.

Example 4 (~)- (2R. 3R. 5R, 1'S.)-2-(1-Acetamido-3-ethv!)Dentv)-3-DroDionv)-Dvrro!idine- 5- carboxylic Acid Hydrochloride.

4A. (iM2R. 3R, 5R, 1'S, 1"R)-and (t)-(2R, 3R, 5R, 1'S, 1"S)-1-Benzyl-2-(1- <BR> <BR> <BR> acetamido-3-ethy!)pentv)-3-(1-hvdroxv)propy)-pyrro!idine-5-c arboxvt)cAc)d-Buty! Ester.

Ethyl magnesium bromide (0. 070mL, 3M in ether) was added to a solution of (i)-(2R, 3R, 5R, 1'S)-1-benzyl-2-(1-acetamido-3-ethyl)pentyl-3-formyl- pyrrolidine-5-carboxylic acid t-butyl ester (18 mg, 0.041 mmole) in 3 mL of tetrahydrofuran. The reaction mixture was maintained at 0 °C, and stirred for 1 hour. The reaction was quenched with aqueous ammonium chloride and partitioned between ethyl acetate and water. The organic layer was dried over MgS04, filtered and concentrated to provide the title product (crude yield : 20mg, 100%).

MS (M+H) + = 475.

4B. (t)- (2R. 3R, 5R, 1'S.)-1-Benzyl-2- (1-acetamido-3-ethyl) penyl-3-propionyl- pyrrolidine-5-carboxylic Acid t-Butyl Ester.

The title compound was prepared according to the method described in Example 2A, substituting ()- (2R, 3R, 5R, 1'S, 1"R)- and ()- (2R, 3R, 5R, 1'S, 1"S)-1- <BR> <BR> <BR> benzyl-2- (1-acetamido-3-ethyl) pentyl-3- (1-hydroxy) propyl-pyrrolidine-5-carboxylic acid t-butyl ester, 20 mg 0.041 mmole), in place of ()- (2R, 3R, 5R, 1'S)-1-benzyl-2- (1-acetamido-3-ethyl) pentyl-3-hydroxymethyl-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 11 mg, 56%).

MS (M+H) + = 473.

4C. ()- (2R, 3R, 5R, 1'S,)-2- (1-Acetamido-3-ethy pentvl-3-propionyl-pvrrolidine- 5-carboxylic Acid t-Butyl Ester.

A mixture of ()- (2R, 3R, 5R, 1'S,)-1-benzyl-2- (1-acetamido-3-ethyl) pentyl-3- propionyl-pyrrolidine-5-carboxylic acid t-butyl ester (11 mg, 0.023 mmole), ammonium formate (250 mg) and palladium (15 mg, 10% on carbon) in ethanol (1.5 mL) was heated at 70 °C for 20 minutes. The reaction was filtered, to remove the catalyst and concentrated. The residue was purified by chromatography on silica gel using 5% methanol/chloroform to provide the title compound (yield : 8.5 mg, 95%).

MS (M+H) + = 383.

4D. ()- (2R, 3R, 5R, 1'S,)-2- (1-Acetamido-3-ethyl) pentyl-3-propionyl-pyrrolidine- 5-carboxylic Acid Hydrochloride.

A solution of ()- (2R, 3R, 5R, 1'S,)-2- (1-acetamido-3-ethyl) pentyl-3- propionyl-pyrrolidine-5-carboxylic acid t-butyl ester (8 mg) was dissolved in 4 N HCI in dioxane (1 mL) and stirred at room temperature for 24 hours. The reaction was concentrated in vacuo to provide the title compound as an off white solid (yield : 8 mg, 100%).

'H NMR (DMSO-d6) 8 8.03 (d, J=14Hz, 1H), 4.41 (m, 1H), 4.20 (m, 1H), 3. 92 (m, 1H), 3.68 (m, 1H), 3.46 (m, 1 H), 2.65 (m, 2H), 2.00 (m, 1H) 1.84 (s, 3H), 1.10-1.35 (m, 9H), 0.95 (t, J=Hz, 3H), 0.81 (t, J=12. 5Hz, 3H), 0.75 (t, J=12.5Hz, 3H) MS: (M-H) = 325, (M+35) + = 361, (2M-H)'= 651; (M+H) + = 327, (2M+1) + = 653, (2M+Na) + = 675.

Example 5 (~)-(2R,3R,5R,1'S)-2-(1-Acetamido-3-ethyl)pentyl-3-(N-methyl carbamoyl)- pyrrolidine-5-carboxylic Acid Hydrochloride.

5A. (~)-(2R,3R,5R,1'S)-1-Benzyl-2-(1-acetamido-3-ethyl)pentyl-3- (N-methyl- carbamov!)pvrro!idine-5-carboxv!ic Acid t-Butvl Ester.

A solution of ()- (2R, 3R, 5R, 1'S)-1-benzyl-2-(1-acetamido-3-ethyl)pentyl-3- carboxyl-pyrrolidine-5-carboxylic acid t-butyl ester (0.175 mmole) and triethylamine (18 mg, 0.175 mmole) in 10 mL THF was cooled in an ice-bath.

Isobutylchloroformate (24 mg, 0.175 mmole) was added and stirred for 30 min.

Then methylamine (2.0 M in THF, 0.35 mL, 0.70 mmole) was added. The mixture was stirred while allowed to warm up to room temperature overnight. The reaction was then diluted with ethyl acetate. The organic layer was washed with water, and brine, dried over MgS04, filtered and concentrated in vacuo. The residue was purified by chromatography on silica gel using 5% methanol/ methylene chloride to provide the title compound, as an oil (yield : 17.2 mg, 21 %).

MS: (M+H) += 474 5B. (i)-(2R. 3R. 5R. 1'S)-2-(1-Acetamido-3-ethyl) pentyl-3-(N-methylcarbamoyl) pyrrolidine-5-carboxylic Acid t-Butyl Ester.

The title compound was prepared according to the method described in Example 1J, substituting ()- (2R, 3R, 5R, 1'S)-1-benzyl-2- (1-acetamido-3- ethyl) pentyl-3-(N-methylcarbamoyl) pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3R, 5R, 1'S)-1-benzyl-2- (1-acetamido-3-ethyl) pentyl-3-methoxy- methyl-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 13 mg, 94%).

MS: (M+H) += 384 5C. (~)-(2R,3R,5R,1'S)-2-(1-Acetamido-3-ethyl)pentyl-3-(N-methyl carbamoyl)- pyrrolidine-5-carboxylic Acid Hydrochloride.

The title compound was prepared according to the method described in Example 1K, substituting ()- (2R, 3R, 5R, 1'S)-2- (1-acetamido-3- ethyl) pentyl-3- (N-methylcarbamoyl) pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3R, 5R, 1'S)-2- (1-acetamido-3-ethyl) pentyl-3- (methoxymethyl)- pyrrolidine-5-carboxylic acid t-butyl ester.

'H NMR (D20): # 4. 43 (t, J= OHz, 1 H), 4.36 (m, 1H), 4.09 (dd, 1 H), 3.08 (q, J=1OHz, 1H), 2.75 (m, 4H), 2.25 (m, 4H), 2.02 (s, 3H), 1.5-1.15 (br, 7H), 0.80 (m, 6H).

MS: (M+H)+ = 328.

Example 6 (~)-(2R,3R,5R,1'S)-2-(1-Acetamido-3-ethyl)pentyl-3-(N-aminoc arbamoyl)- pyrrolidine-5-carboxylic Acid Hydrochloride.

6A. (~)-(2R,3R,5R,1'S)-1-benzyl-2-(1-acetamido-3-ethyl)pentyl-3- (N-(t- butoxycarbonyl)aminocarbamoyl)-pyrrolidine-5-carboxylic Acid t-But I Ester.

A solution of ()- (2R, 3R, 5R, 1'S)-1-benzyl-2-(1-acetamido-3-ethyl)pentyl-3- carboxyl-pyrrolidine-5-carboxylic acid t-butyl ester (60 mg, 0.13 mmole), t-butyl carbazate (21 mg, 0.16 mmole), 1- (3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC, 31mg, 0.16 mmole) and 1-hydroxybenzotriazole (9 mg, 0.065 mmole) in 3 mL anhydrous THF was stirred at room temperature for 6 hours. The reaction was then diluted with ethyl acetate. The organic layer was washed with water and brine, dried over MgS04, filtered and concentrated in vacuo. The residue was purified by chromatography on silica gel using 2% methanol/methylene chloride to provide the title compound, as an oil (yield : 45.6 mg, 61%).

MS: (M+H) += 575 6B. (~)-(2R,3R, 5R. 1'S)-2-(1-Acetamido-3-ethyl) pentyl-3-(N-(t- butoxvcarbonyl) aminocarbamovl)pprrrolidine-5-carboxylic Acid t-Butvl Ester.

The title compound was prepared according to the method described in Example 1J, substituting ()- (2R, 3R, 5R, 1'S)-1-benzyl-2- (1- acetamido-3-ethyl) pentyl-3- (N- (t-butoxycarbonyl) aminocarbamoyl) pyrrolidine-5- carboxylic acid t-butyl ester in place of ()- (2R, 3R, 5R, 1'S))-1-benzyl-2-(1- <BR> <BR> <BR> acetamido-3-ethyl) pentyl-3-methoxymethyl-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 28 mg, 75%).

MS: (M+H) += 484 6C. ()- (2R. 3R. 5R. 1'S)-2-(1-Acetamido-3-ethyl)pentyl-3-(N-aminocarbamoyl)- pyrrolidine-5-carboxylic Acid Hydrochloride.

The title compound was prepared according to the method described in Example 1K, substituting ()- (2R, 3R, 5R, 1'S)-2-(1-acetamido-3-ethyl)pentyl-3-(N- (t-butoxycarbonyl) aminocarbamoyl)-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3R, 5R, 1'S)-2-(1-acetamido-3-ethyl)pentyl-3-(methoxymethyl)- pyrrolidine-5-carboxylic acid t-butyl ester.

1H NMR (D20): 8 4.32 (m, 2H), 4.18 (dd, 1H), 3.14 (q, J=8.4Hz, 1H), 2.75 (m, 1H), 2.26 (m, 1H), 2.01 (s, 3H), 1.50-1.15 (m, 7H), 0.80 (q, J=7.5Hz, 6H) MS: (M+H)+ = 329 Example 7 <BR> <BR> <BR> ()- (2R, 3R. 5R, 1'S)-2-1-Acetamido-3-ethvl) pentyl-3-ethoxvcarbonyl-pyrrolidine-5- carboxylic Acid Hydrochloride.

7A. $(~)-(2R,3R,5R,1'S)-1-Benzyl-2-(1-acetamido-3-ethyl)pentyl-3 - ethoxycarbonyl-pyrrolidine-5-carboxylic Acid t-Butyl Ester.

A solution of (~)-(2R, 3R, 5R, 1'S)-1-benzyl-2-(1-acetamido-3-ethyl)pentyl-3- carboxyl-pyrrolidine-5-carboxylic acid t-butyl ester (42 mg, 0.091 mmole), ethanol (0.5 mL), 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC, 36 mg, 0.188 mmole) and 1-hydroxybenzotriazole (7 mg, 0.05 mmole) in 2 mL anhydrous THF was stirred at room temperature overnight. The reaction was then diluted with ethyl acetate. The organic layer was washed with water, and brine, dried over MgS04, filtered and concentrated in vacuo. The residue was purified by chromatography on silica gel using 2% methanol/methylene chloride to provide the title compound, as an oil (yield: 36 mg, 33%). aH NMR (CDCI3) : 8 7.50-7.20 (br, 5H), 5.12 (d, J=9Hz, 1H), 4.60-4.30 (br, 2H), 4.14 (q, J=6Hz, 2H), 4.08 (m, 1 H), 3.85 (br, 1H), 3.72 (m, 1 H), 3.40 (m, 1 H), 2.75 (m, 1 H), 2.32 (m, 1H), 1.97 (s, 3H), 1.40 (s, 9H), 1.37 (t, J=6Hz, 3H), 1.20- 1.50 (m, 7H), 0.83 (m, 6H).

Mass spectrum: (M+H) + = 489 7B. (~)- (2R. 3R. 5R. 1'S)-2- (1-Acetamido-3-ethyl) pentyl-3-ethoxycarbonyl- pyrrolidine-5-carboxylic Acid t-Butyl Ester.

The title compound was prepared according to the method described in Example 1J, substituting ()- (2R, 3R, 5R, 1'S)-1-benzyl-2-(1-acetamido-3-ethyl)- pentyl-3-ethoxycarbonyl-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3R, 5R, 1'S)-1-benzyl-2-(1-acetamido-3-ethyl)pentyl-3-methoxymethyl- pyrrolidine-5-carboxylic acid t-butyl ester.

Mass spectrum: (M+H) + = 399.

7C. (~)-(2R,3R,5R,1'S)-2-(1-Acetamido-3-ethyl)pentyl-3-ethoxycar bonyl- pyrrolidine-5-carboxylic Acid Hydrochloride.

The tit!ecompoundwaspreparedaccording to the method described in Example 2E, substituting ()- (2R, 3R, 5R, 1'S)-2- (1-acetamido-3- ethyl) pentyl-3-ethoxycarbonyl-pyrrolidine-5-carboxylic acid t-butyl ester in place of (~)- (2R, 3R, 5R, 1'S)-2-(1-acetamido-3-ethyl)pentyl-3-methoxycarbonyl-pyrroli dine- 5-carboxylic acid t-butyl ester.

1H NMR (D20) : # 4.35 (m, 1H), 4.20 (q, J=7.5Hz, 2H), 3.87-3.55 (m, 2H), 3.20 (q, J=7.5Hz, 1H), 2.67 (m, 1H), 2.42 (m, 1H), 2.02 (s, 3H), 1.24 (t, J=7.5Hz, 3H), 1.54-1. 15 (m, 7H), 0.82 (m, 6 H).

Mass spectrum: (M+H) + = 343, (M-H)'= 341.

Example 8 (i)-(2R. 3R. 5R. 1'S)-2-(1-Acetamido-3-ethyl) pentyl-3-acetyl-pyrrolidine-5- carboxvtic Acid Hydrochloride.

8A. (~)-(2R,3R,5R,1'S,1"R)-and (~)-(2R,3R,5R,1'S,1"S)-1-Beznyl-2-(1- <BR> <BR> <BR> acetamido-3-ethyl)pentyl-3-(1-hydroxy)ethyl-pyrrolidine-5-ca rboxylic Acid t-Butyl Ester.

The title compound was prepared according to the method described in Example 4A substituting methyl magnesium bromide in place of ethyl magnesium bromide.

8B (i)-(2R. 3R. 5R. 1'S)-1-Benzyl-2-(1-acetamido-3-ethyl)pentyl-3-acetyl- pyrrolidine-5-carboxylic Acid t-Butyl Ester.

The title compound was prepared according to the method described in Example 2A, substituting ()- (2R, 3R, 5R, 1'S, 1"R)- and ()- (2R, 3R, 5R, 1'S, 1"S)-1- <BR> <BR> <BR> <BR> benzyl-2- (l-acetamido-3-ethyl) pentyl-3- (l-hydroxy) ethyl-pyrrolidine-5-carboxylic acid t-butyl ester, prepared according to the procedure described in Example 8A, in place of ()- (2R, 3R, 5R, 1'S)-1-benzyl-2-(1-acetamido-3-ethyl)pentyl-3-hydroxy- methyl-pyrrolidine-5-carboxylic acid t-butyl ester.

1H NMR (CDCl3) # 5.00 (d, J=9.7Hz, 1H), 3.94 (m, 2H), 3.68 (m, 1H), 3.55 (m, 1H), 2.64 (m, 1H), 2.32 (m, 1H), 2.29 (s, 3H), 2.20 (m, 1H), 1.94 (s, 3H), 1.43 (s, 9H), 1.15- 11.35 (m, 7H), 0.80 (m, 6H).

MS : (M+H)+=459 8C. )- (2R, 3R, 5R. 1'S)-2- (1-Acetamido-3-ethyl) pentyl-3-acetyl-pyrrolidine-5- carboxylic Acid t-Butyl Ester.

The title compound is prepared according to the method described in Example 4C, substituting ()- (2R, 3R, 5R, 1'S)-1-benzyl-2-(1-acetamido-3- ethyl) pentyl-3-acetyl-pyrrolidine-5-carboxylic Acid t-butyl ester., prepared according to the procedure described in Example 8B, in place of ()- (2R, 3R, 5R, 1'S)-1-benzyl-2-(1-acetamido-3-ethyl)pentyl-3-propionyl-pyrr olidine-5- carboxylic acid t-butyl ester.

MS: (M+H)+=369 8D. ()- (2R. 3R, 5R, 1'S)-2- (l-Acetamido-3-ethvl) pentyl-3-acetyl-pyrrolidine-5- carboxylic Acid Hydrochloride.

The title compound is prepared according to the method described in Example 1K, substituting (~)-(2R,3R,5R,1'S)-2-(1-acetamido-3-ethyl)pentyl-3- acetyl-5-carboxylic acid t-butyl ester in place of ()- (2R, 3R, 5R, 1'S)-2- (1- acetamido-3-ethyl) pentyl-3- (methoxymethyl)-pyrrolidine-5-carboxylic acid t-butyl ester.

'H NMR (DMSO-d6) 8 8.20 (m, 1H), 4.35 (m, 1H), 4.15 (m, 1H), 4.03 (m, 1H), 2.43 (m, 1H), 2.03 (m, 1H), 1.91 (s, 3H), 1.77 (s, 3H), 1.55 (m, 1H), 1.46 (m, 1H), 1.35 (m, 2H), 1.12 (m, 4H), 0.84 (m, 3H), 0.79 (m, 3H) MS: (M+H) +=314, (M-H)'=312 Example 9 ()- (2S. 3R. 5R. 1'S)-2- (1-Acetamido-3-ethvl) penyl-3-amino-pyrrolidine-5- carboxylic Acid Dihydrochloride.

9A. (~)-(2S,3R,5R)- and (~)-(2S,3S,5R)-1-Benzyl-2-vinyl-3-carboxyl- yrrrolidine-5-carboxylic Acid t Butyl Ester.

A solution of (~)-(2S,3R,5R)- and (~)-(2S, 3S, 5R)-1-benzyl-2-vinyl-3-formyl- pyrrolidine-5-carboxylic acid t-butyl ester (10 g, 31.7 mmole) (8: 1 ratio), in 39 mL of ethanol was prepared. The solution was treated with a suspension of silver oxide (8.83 g, 38.1 mmole) and potassium hydroxide (10.86 g, 194 mmole) in 65 mL of water. The reaction was stirred at room temperature for 1 hour and filtered through a pad of Celte@. The ethanol was removed in vacuo. The aqueous solution was acidified with acetic acid to about pH 4. The acidic solution was extracted with ethyl acetate. The organic layer was washed with brine, dried over Na2SO4, filtered and concentrated to provide the title compound as a brownish oil (crude yield : 8.2 g, 77%). The crude acid was used for the next step without further purification.

MS (M+H) + = 332, (M-H)-= 330.

9B. ()- (2S, 3R. 5R)-1-Benzyl-2-vinyl-3-benzyloxycarbonylamino-pyrrolidine-5- carboxylic Acid t-Butyl Ester.

A mixture of ()- (2S, 3R, 5R)- and ()- (2S, 3S, 5R)-1-benzyl-2-vinyl-3- carboxyl-pyrrolidine-5-carboxylic acid t-butyl ester (1.0 g, 3.02 mmole), diphenylphosphoryl azide (0.83 g, 3.32 mmole), benzyl alcohol (0.36 g, 4.53 mmole) and triethylamine (0.32 g, 3.32 mmole) in 30 mL of toluene was heated at reflux for 16 hours. The solvent was evaporated and the residue was purified by chromatography on silica gel using 10% ethyl acetate/hexanes to provide the title compound as a colorless oil (yield : 0.86 g, 65%).

'H NMR (CDC13) 8 7.20-7.40 (m, 10H), 5.70 (m, 2H), 5.10-5.23 (m, 3H), 4.10 (m, 1 H), 3.85 (m, 1 H), 3.62 (m, 1 H), 3.45 (m, 2H), 2.50 (m, 1 H), 1.70 (m, 1 H), 1.41 (s, 9H).

MS (M+H) + = 437.

9C. (t)- (2R, 3R. 5R)-1-Benzvl-2-formyl-3-benzyloxvcarbonylamino-pyrrolidine-5 - carboxylic Acid t-Butyl Ester.

Osmium tetroxide (3 crystals) was added to a stirred solution of (i)- (2S, 3R, 5R)-1-benzyl-2-vinyl-3-benzyloxycarbonylamino-pyrrolidine-5- carboxylic acid t-butyl ester (1.10 g, 2.52 mmole), N-methylmorpholine N-oxide (0.95 g, 8.07 mmole), in 27 mL of acetone/water (8: 1), maintained at room temperature. After 6 hours, 10% aqueous Na2S203 was added and stirring continued for an additional 15 minutes. The reaction was extracted with dichloromethane and the organic layer was concentrated to provide the crude diol intermediate. The diol product was used in the next step without additional purification.

MS (M+H) + = 471.

Sodium periodate (1.0 g, 4.52 mmole) was added in portions to a stirred solution of the crude diol (-1. 25 g, 2.66 mmole) in 21 mL of THF/water (6 : 1). The reaction was stirred for 1 hour then diluted with ethyl acetate. The organic layer was washed with water and brine, dried over MgS04, filtered and concentrated.

The residue was purified by chromatography on silica gel using 15% ethyl acetate/hexanes to provide the title compound as a colorless oil (yield : 0.66 g, 60%).

'H NMR (CDC13) 8 9.44 (d, J=1.2Hz, 1H), 7.20-7.40 (m, 10H), 5.98 (d, J=14Hz, 1H), 5.10 (m, 2H), 4.45 (m, 1H), 3.90 (m, 2H), 3.70 (m, 1H), 3.60 (m, 1H), 2.43 (m, 1H), 1.70 (m, 1H), 1.45 (s, 9H), MS (M+H) + = 439.

9D. (i)-(2R, 3R. 5R, 1'R)-and (i)-(2R. 3Rw5R. 1'S)-1-Benzvl-2-(1-hydroxy-3- <BR> <BR> <BR> ethvl) pentyl-3-benzvloxycarbonylamino-pyrrolidine-5-carboxylic Acid t-Buyl Ester.

1-Bromo-2-ethylbutane (1.7 g, 10.3 mmole) was added a solution of dibromoethane (3 drops) in 15 mL of dry THF, under argon, in a flask charged with magnesium (0.25 g, 10.3 mmole). The reaction mixture was heated at reflux for 45 minutes, until most of the magnesium reacted. The solution was allowed to cool to room temperature and transferred via cannula to a suspension of CuBr-SMe2 (2.12 g, 10.3 mmole) in 15 mL of dry THF, maintained under argon, at-10 °C. The mixture was stirred for 0.5 hours until the solution turned dark. A solution of ()- (2R, 3R, 5R)-1-benzyl-2-formyl-3-benzyloxycarbonylamino- pyrrolidine-5-carboxylic acid t-butyl ester (0.45 g, 1.03 mmole) in 10 mL of THF was added dropwise and stirred for 1.5 hours, while maintaining the temperature at 0 °C. The reaction was quenched with aqueous ammonium chloride, diluted with ethyl acetate, washed with water and brine, dried over MgS04, filtered and concentrated. The residue was purified by chromatography on silica gel using 10% ethyl acetate/hexanes to provide alcohol adducts as a pale yellow oil (yield: 160 mg, 30%).

'H NMR (CDC13) b 7.20-7.40 (m, 10H), 6.10 (d, J=14Hz, 1H), 5.10 (m, 2H), 4.22 (m, 1 H), 4.01 (m, 1 H), 3.71 (m, 1 H), 3.65 (m, 2H), 3.55 (m, 1 H), 3.20 (m, 1 H), 2.00-2.30 (m, 2H), 1.45 (s, 9H), 1.15-1.40 (m, 7H), 0.84 (m, 6H) MS (M+H) + = 525.

9E. (i)-(2 R ; 3R. 5R)-1-Benzyl-2-(1-oxo-3-ethvl) pentyl-3- benzyloxvcarbonylamino-pyrrolidine-5-carboxylic Acid t-Butyl Ester.

A solution of oxalyl chloride (0.29 ml, 2 M in CH2CI2) in 5 mL of dry dichloromethane was prepared and maintained under a nitrogen atmosphere at- 78 °C. DMSO (90 mg, 1.14 mmole) was added to the solution. The mixture was stirred for 15 minutes. The alcohol adduct, prepared above, (150 mg, 0.286 mmole), in 5 mL of dichloromethane, was added dropwise to the cold (-78 °C) reaction mixture. The solution was stirred, at-78 °C, for 1 hour. Triethylamine (250 mg, 2.29 mmole) was added slowly. The reaction was allowed to slowly warm to room temperature and then diluted with dichloromethane. The organic layer was washed with water and brine, dried over MgS04, filtered and concentrated. The residue was purified by chromatography on silica gel using 5% ethyl acetate/hexanes to provide the title compound (yield : 100 mg, 67%). aH NMR (CDCl3) 87. 35 (m, 10H), 5.10 (m, 2H), 4.28 (m, 1H), 3.95 (m, 2H), 2.60 (m, 1H), 2.40 (m, 1H), 2.03 (m, 1H), 1.70 (m, 2H), 1.45 (s, 9H), 1.10-1.30 (m, 7H), 0.70 (m, 6H) MS (M+H) + = 523.

9F. (i)-(2S, 3R. 5R. 1'R)-and (i)-(2S. 3R. 5R, 1'S)-1-Benzyl-2-(1-amino-3- ethVl ! pentyi-3-benzvloxycarbonylamino-pyrrolidine-5-carboxylic Acid t-Butvl Ester.

A mixture of ()- (2R, 3R, 5R)-1-benzyl-2- (1-oxo-3-ethyl) pentyl-3-benzyloxy- carbonylamino-pyrrolidine-5-carboxylic acid t-butyl ester (90 mg, 0.172 mmole), ammonium acetate (400 mg, 5.17 mmole) and sodium cyanoborohydride (65 mg, 1.03 mmole) in 5 mL in methanol was heated at reflux for 18 hours. Additional portions of ammonium acetate and sodium cyanoborohydride were added and heating continued for an additional 2 hours. The reaction was quenched with 1 N sodium hydroxide, and diluted with dichloromethane. The organic layer was washed with water and brine, dried over MgS04, filtered and concentrated. The residue was purified by chromatography on silica gel using 1: 1 ethyl acetate/hexanes followed by 5% methanol/dichloromethane to provide the title compounds. (yield : 58 mg, 64%) MS (M+H) + = 524.

9G. (~)-(2S,3R,5R,1'R)- and (~)-(2S,3R,5R,1'S)-1-Benzyl-2-(1-acetamido-3- <BR> <BR> <BR> <BR> ethyl)pentyl-3-benzyloxycarbonylamino-pyrrolidine-5-carboxyl ic Acid t-Butyl Ester.

A solution of ()- (2S, 3R, 5R, 1'R)- and ()- (2S, 3R, 5R, 1'S)-1-benzyl-2-(1- amino-3-ethyl)pentyl-3-benzyloxycarbonylamino-pyrrolidine-5- carboxylic acid t- butyl ester (50 mg, 0.096 mmole) and acetic anhydride (117 mg, 1.15 mmole) in 5 mL of dichloromethane was stirred for 1 hour at room temperature. The solvent was evaporated in vacuo and the residue purified by chromatography on silica gel using 30-50% ethyl acetate/hexanes to provide the title compound as a colorless oil (yield : 51 mg, 97%).

'H NMR (CDC13) 8 7.72-7.35 (m, 10H), 5.82 (d, J=14Hz, 1H), 5.10 (m, 2H), 4.38 (m, 1H), 4.15 (m, 2H), 3.63 (m, 1H), 3.38 (m, 1H), 3.10 (m, 1H), 2.15 (m, 1H), 2.00 (s, 3H), 1.65 (m, 1 H), 1.42 (s, 9H), 1. 20-1.35 (m, 7H), 0.80 (m, 6H) MS (M+H) + = 567.

9H. ()- (2S. 3R, 5R, 1'S)-2- 1-Acetamido-3-ethyl) pentyl-3-amino-pvrrolidine-5- carboxylic Acid t-Butyl Ester.

A solution of ()- (2S, 3R, 5R, 1'R)- and ()- (2S, 3R, 5R, 1'S)-1-benzyl-2-(1- acetamido-3-ethyl) pentyl-3-benzyloxycarbonylamino-pyrrolidine-5-carboxylic acid t-butyl ester (49 mg, 0.087 mmole), ammonium formate (150 mg, 0.22 mmole) and 10% palladium on activated carbon in ethanol (5 mL) was heated at 80 °C for 45 minutes. After filtration to remove the catalyst, the solvent was removed. The residue was purified by chromatography on silica gel using 5-10% methanol/dichloromethane to furnish the diastereomers, ()- (2S, 3R, 5R, 1'S) (19 mg) and (~)-(2S, 3R, 5R, 1'R) (8.6 mg) of 2- (1-acetamido-3-ethyl) pentyl-3-amino- pyrrolidine-5-carboxylic acid t-butyl ester.

'H NMR (CDC13) 8 6.00 (d, J=14Hz, 1H), 3.90 (m, 1H), 3.73 (m, 1H), 3.49 (m, 1H), 3.10 (m, 1 H), 2.48 (m, 1H), 2.03 (s, 3H), 1.82 (m, 1H), 1.48 (s, 9H), 1.15- 1.42 (m, 7H), 0.85 (m, 6H) MS (M+H) + = 342.

91. (~)-(2S,3R,5R,1'S)-2-(1-Acetamido-3-ethyl)pentyl-3-amino-pyr rolidine-5- carboxylic Acid Dihydrochloride.

A solution of ()- (2S, 3R, 5R, 1'S)-2-(1-acetamido-3-ethyl)pentyl-3-amino- pyrrolidine-5-carboxylic acid t-butyl ester (17 mg, 0.050 mmole) in 1 mL of 6 N HCI was stirred at room temperature for 3 hours. The solvent was removed under high vacuum to provide the title compound as a white solid (yield: 15 mg, 100%) 'H NMR (d6-DMSO) 8 8.28 (bs, 1H), 7.90 (d, J=Hz, 1H), 4.71 (d, J=14Hz, 1 H), 4.39 (m, 1 H), 4. 10(m, 1H), 3.92 (m, 1H), 3.08 (m, 1H), 2.64 (m, 1H), 2.31 (m, 1H), 1.95 (m, 1H), 1.88 (s, 3H), 1.50 (m, 1H), 1.10-1.40 (m, 7H), 0.72-0.90 (m, 6H).

MS (M+H) + = 286.

Example 10 (i)-(2S. 3R. 5R. 1'S)-2-(1-Acetamido-3-ethyl) pentvl-3-acetamido-nyrrolidine-5- carboxylic Acid Hydrochloride.

10A. ()-(2S.3R.5R.1'S1-Benzv)-2-(1-acetamido-3-ethvnDentv)-3-amin o- pyrrolidine-5-carboxylic Acid t-Butyl Ester.

A solution of ()- (2S, 3R, 5R, 1'S)-1-benzyl-2-(1-acetamido-3-ethyl)pentyl-3- benzyloxycarbonylamino-pyrrolidine-5-carboxylic acid t-butyl ester (50 mg, 0.88 mmole) was stirred with 10% palladium on carbon (5 mg) in 50 mL of ethyl acetate under 1 atmosphere of hydrogen for 45 minutes. The reaction was filtered and concentrated to provide the title compound as an oil (crude yield : 35 mg, 92%).

MS (M+H) + = 431.

10B. ()- (2S, 3R, 5R, 1'S)-l-Benzyl-2-Cl-acetamido-3-ethyl) pentyl-3-acetamido- pyrrolidine-5-carboxylic Acid t-Butyl Ester.

A solution of ()- (2S, 3R, 5R, 1'S)-1-benzyl-2-(1-acetmaido-3-ethyl)pentyl-3- amino-pyrrolidine-5-carboxylic acid t-butyl ester (35 mg, 0.080 mmole) was reacted with acetic anhydride (0.05 mL) in 8 mL of dichloromethane for 1 hour.

The reaction was concentrated and the residue purified by chromatography on silica gel using 50% ethyl acetate/hexanes followed by 3% methanol/dichloromethane to provide the title compound (yield : 30 mg, 80%).

1H NMR (CDCl3) 87. 20-7.35 (m, 5H), 6.62 (d, J=14Hz, 1H), 5.34 (d, J=14Hz, 1H), 4.42 (m, 2H), 4.20 (m, 1H), 3.68 (m, 1H), 3.42 (m, 1H), 3.10 (m, 1 H), 2.18 (m, 2H), 2.02 (s, 3H), 1.96 (s, 3H), 1.45 (s, 9H), 1.25-1.42 (m, 7H), 0.85 (m, 6H).

MS (M + H) + = 474.

10C. ()- (2S. 3R. 5R, 1'S)-2- (1-Acetamido-3-ethyl) penyl-3-acetamido-pvrrolidine- 5-carboxylic Acid t-Butyl Ester.

The title compound was prepared according to the method described in Example 1J, substituting ()- (2S, 3R, 5R, 1'S)-1-benzyl-2-(1-acetamido-3- ethyl) pentyl-3-acetamido-pyrrolidine-5-carboxylic acid t-butyl ester in place (~)- (2R, 3R, 5R, 1'S)-1-benzyl-2- (1-acetamido-3-ethyl) pentyl-3-methoxymethyl- pyrrolidine-5-carboxylic acid t-butyl ester. The residue was purified by chromatography on silica gel using 5% methanol/dichloromethane to provide the title compound (yield : 11.5 mg, 50%).

1H NMR (CDC13) 5 6.20 (d, J=14Hz, 1H), 5.94 (d, J=14Hz, 1H), 4.24 (m, 1H), 4.08 (m, 1 H), 3.95 (m, 1H), 3.75 (m, 1 H), 3.18 (m, 1H), 2.45 (m, 1H), 2.02 (s, 3H), 1.96 (s, 3H), 1.82 (m, 1H), 1.49 (s, 9H), 1.20-1.42 (m, 7H), 0.85 (m, 6H).

MS (M + H) + = 384.

10D (M2S.3R.5R.1'S)-2-n-Acetamido-3-ethv!)Dentvt-3-acetam)do-Dvr ro!idine- 5-carboxylic Acid Hydrochloride.

The title compound was prepared according to the method described in Example 1K, substituting ()- (2S, 3R, 5R, 1'S)-2- (1-acetamido-3-ethyl) pentyl-3- acetamido-pyrrolidine-5-carboxylic acid t-butyl ester (11.0 mg, 0.029 mmole) in place of ()- (2R, 3R, 5R, 1'S)-2-(1-acetamido-3-ethyl)pentyl-3-(methoxymethyl)- pyrrolidine-5-carboxylic acid t-butyl ester (yield : 11.0 mg, 100%).

'H NMR (d6-DMSO) 8 8.15 (d, J=14Hz, 1H), 8.05 (d, J=14Hz, 1H), 4.35 (m, 1H), 4.28 (m, 1H), 4.19 (m, 1H), 3.59 (m, 1H), 1.90 (s, 3H), 1.81 (s, 3H), 1.15- 1.40 (m, 7H), 0.80 (m, 6H).

MS: (M-H)-= 326, (M+35) + = 362; (M+H) + = 328, (M+23) + = 350.

Example 11 (~)-(2S,3R,5R,1'S)-2-(1-Acetamido-3-ethyl)pentyl-3-methoxyca rbonylamino- pyrrolidine-5-carboxylic Acid Hydrochloride.

11A.f)-(2S.3R.5R.1'S)-1-Benzv!-2-M-acetamido-3-ethv!)Dentv)- 3-methoxv-<BR> <BR> carbonylamino-pyrrolidine-5-carboxylic Acid t-Butyl Ester.

A solution of ()- (2S, 3R, 5R, 1'S)-1-benzyl-2- (1-acetamido-3-ethyl) pentyl-3- amino-pyrrolidine-5-carboxylic acid t-butyl ester is reacted with methyl chloroformate and triethylamine in dichloromethane. The reaction is partitioned between dichloromethane and water. The organic layer is concentrated to provide the title compound.

11B. ()- (2S, 3R, 5R, 1'S)-21-Acetamido-3-ethyl) pentyl-3- methoxycarbonylamino-pvrrolidine-5-carboxylic Acid t-Butyl Ester.

The title compound is prepared according to the method described in Example 1J, substituting ()- (2S, 3R, 5R, 1'S)-1-benzyl-2-(1-acetamido-3-ethyl)- pentyl-3-methoxycarbonylaminopyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3R, 5R, 1'S)-1-benzyl-2- (1-acetamido-3-ethyl) pentyl-3-methoxymethyl- pyrrolidine-5-carboxylic acid t-butyl ester.

11C. (~)-(2S,3R,5R,1'S)-2-(1-Acetamido-3-ethyl)pentyl-3- methoxvcarbonvlamino-pyrrolidine-5-carboxylicAcid Hydrochloride.

The title compound is prepared according to the method described in Example 1K, substituting ()- (2S, 3R, 5R, 1'S)-2-(1-acetamido-3-ethyl)pentyl-3- methoxycarbonylamino-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3R, 5R, 1'S)-2- (1-acetamido-3-ethyl) pentyl-3- (methoxymethyl)-pyrrolidine-5- carboxylic acid t-butyl ester.

Example 12 (~)-(2R,3R,5R,1'S)-2-(1-Acetamido-3-ethyl)pentyl-3-(imidazol -4-yl)-5-carboxylic AcidDihydrochloride.

12A. (i)-(2R, 3R, 5R, 1'S)-1-Benzyl-2-(1-acetamido-3-ethyl) pentyl-3-diazoacetyl- 5-carboxylic Acid t-Butyl Ester.

A solution of ()- (2R, 3R, 5R, 1'S)-1-benzyl-2- (1-acetamido-3-ethyl) pentyl-3- carboxyl-pyrrolidine-5-carboxylic acid t-butyl ester (405.3 mg, 0.88 mmol) and N- methylmorpholine (106 pI, 0.96 mmol) in THF (20 ml) was reacted with isobutyl chloroformate (96 µl, 0.93 mmole) at-10 °C for 30 minutes. To the reaction flask was cannulated a distille diazomethane solution in ether prepared from the reaction of diazald (2.4 g) in ether (60 ml) with a solution of potassium hydroxide (2.4 g) in ethanol (15 ml) and water (15 ml). The reaction was stirred for 3 hours at room temperature then diluted with ether. The organic layer was washed with brine, dried (Na2SO4) and concentrated to give the title compound as a thick oil (430.4 mg).

12B. (i)-(2R. 3R. 5R. 1'S)-1-Benzvl-2-(1-Acetamido-3-ethyl) pentyl-3-bromoacetyl- 5-carboxviic Acid t-Butyl Ester.

A solution of ()- (2R, 3R, 5R, 1'S)-l-benzyl-2- (l-acetamido-3-ethyl) pentyl-3- diazoacetyl-5-carboxylic acid t-butyl ester (427.4 mg, 0.88 mmol) in dioxane (50 ml) was reacted with hydrobromic acid (0.25 ml, 2.2 mmol) at 0 °C for 0.5 hours.

The reaction was quenched with saturated aqueous sodium bicarbonate (25 ml) and concentrated in vacuo. The residual aqueous layer was extracted with dichloromethane (3x50 ml). The combined organic layers were washed with brine, dried over sodium sulfate, filtered and concentrated in vacuo. The residue was purified by chromatography on silica gel using 5% methanol in dichloromethane to provide the title compound as a white foamy solid (379.3 mg, 80.2 %).

MS: (M+H) += 539.

12C. (~)-(2R,3R,5R,1'S)-1-Benzyl-2-(1-acetamido-3-ethyl)pentyl-3- (imidazol-4- yl)-5-carboxylic Acid t-Butyl Ester.

()- (2R, 3R, 5R, 1'S)-1-benzyl-2- (1-acetamido-3-ethyl) pentyl-3-bromoacetyl- 5-carboxylic acid t-butyl ester (60 mg, 0.112 mmol) was treated with formamidine acetate (120 mg, 1.15 mmol) in liquid ammonia and heated at 45 °C in a sealed tube for 20 h. The reaction was concentrated in vacuo. The residue was treated with aqueous NaHCO3 and extracted with dichloromethane (5x20 ml). The combined organic layers were washed with brine, dried over sodium sulfate, filtered and concentrated in vacuo. The residue was purified by chromatography on silica gel using 5% methanol in dichloromethane to provide the title compound as a white solid (21.2 mg, 39.4 %).

MS: (M+H) += 483.

12D. (~)-(2R,3R,5R,1'S)-2-(1-Acetamido-3-ethyl)pentyl-3-(imidazol -4-yl)-5- carboxylic Acid t-Butyl Ester.

The title compound was prepared according to the method described in Example 1J, substituting ()- (2R, 3R, 5R, 1'S)-1-benzyl-2-(1-acetamido-3-ethyl) pentyl-3-(imidazol4-yl)-5-carboxylic acid t-butyl ester in place of (t)- (2R, 3R, 5R, 1'S)-1-benzyl-2- (1-acetamido-3-ethyl) pentyl-3-methoxy-methyl- pyrrolidine-5-carboxylic acid t-butyl ester (yield : 12.9 mg, 66.2%).

'H NMR (CDC13) : # 0. 75-0.81 (m, 6H), 1.17-1.42 (m, 7H), 1.47 (s, 9H), 2.03 (s, 3H), 2.66 (m, 1 H), 3.50 (m, 1 H), 3.73 (m, 1 H), 3.86 (m, 1 H), 4.06 (m, 1 H), 7.04 (br s, 1 H), 7.86 (br s, 1 H).

MS: (M+H)+= 393.

12E. (i)-(2R. 3R. 5R, 1'S)-2-(1-Acetamido-3-ethyl) pentyl-3-(imidazol4-yl)-5- carboxylic Acid Dihydrochloride.

The title compound was prepared according to the method described in Example 1K, substituting ()- (2R, 3R, 5R, 1'S)-2-(1-acetamido-3-ethyl)pentyl-3- (imidazol-4-yl)-5-carboxylic acid t-butyl ester in place of ()- (2R, 3R, 5R, 1'S)-2- (1- <BR> <BR> <BR> acetamido-3-ethyl) pentyl-3- (methoxymethyl)-pyrrolidine-5-carboxylic acid t-butyl ester to provide the title compound solid (yield : 12.0 mg, 96.0%).

1H NMR (DMSO-d6) : 5 0.67 (t, J = 7 Hz, 3H), 0.75 (t, J = 7 Hz, 3H), 1.11 (m, 3H), 1.23 (m, 4H), 1.78 (s, 3H), 2.33 (m, 1 H), 2.70 (m, 1H), 3.69 (dt, 1H), 3.95 (dd, 1 H), 4.29 (m, 1 H), 4.48 (dd, 1 H), 7.63 (s, 1 H), 8.28 (d, J = 9 Hz, 1 H), 9.06 (s, 1H).

MS : (M+H) += 337.

Example13 (i)-(2R. 3R. 5R. 1'S)-2- (1-Acetamido-3-ethvl) pentyl-3- (oxazol-2-yl)-pyrrolidine-5- carboxylic Acid Dihvdrochloride.

13A. (~)-(2R,3R,5R,1'S)-1-Benzyl-2-(1-acetamido-3-ethyl)pentyl-3- (N-(2- hydroxyethyl) carbamoyl)-pyrrolidine-5-carboxylic Acid t-Butyl Ester.

The title compound is prepared according to the method described in Example 5A, substituting ethanolamine for N-methylamine hydrochloride.

13B. (~)-(2R,3R,5R,1'S)-1-Benzyl-2-(1-acetamido-3-ethyl)pentyl-3- (oxazolin-2- yl)-pyrrolidine-5-carboxylic Acid t-Butyl Ester.

A solution of ()- (2R, 3R, 5R, 1'S)-1-benzyl-2- (1-acetamido-3-ethyl) pentyl-3- (N- (2-hydroxyethyI) carbamoyl)-pyrrolidine-5-carboxylic acid t-butyl ester, triethylamine (4 eq.), carbon tetrachloride (3.5 eq.) in acetonitrile is reacted with triphenylphosphine (3.15 eq.) for 16h at room temperature. The reaction is concentrated in vacuo. The residue is partitioned between ethyl acetate and water. The organic layer is washed with water, and brine, dried over MgS04, filtered and concentrated in vacuo. The residue is purified by chromatography on silica gel using ethyl acetate/hexanes to provide the title compound. 13C. (i)-(2R. 3R. 5R. 1'S)-1-Benzyl-2-(1-acetamido-3-ethvl) nentyl-3-(oxazol-2-yl)- pyrrolidine-5-carboxylic Acid t-Butvl Ester.

A solution of ()- (2R, 3R, 5R, 1'S)-1-benzyl-2- (1-acetamido-3-ethyl) pentyl-3- (oxazolin-2-yl)-pyrrolidine-5-carboxylic acid t-butyl ester is reacted with nickel peroxide in cyclohexane according to the method described by Meyer in J. Org.

Chem. 1979,497-501 to provide the title compound.

13D. (t)- (2R. 3R. 5R, 1'S)-2- (1-Acetamido-3-ethyl) pentyl-3- (oxazol-2-yl)- pyrrolidine-5-carboxylic Acid t-Butyl Ester.

The title compound is prepared according to the method described in Example 1J, substituting ()- (2R, 3R, 5R, 1'S)-1-benzyl-2- (1-acetamido-3-ethyl)- pentyl-3- (oxazol-2-yl)-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3R, 5R, 1'S)-1-benzyl-2-(1-acetamido-3-ethyl)pentyl-3-methoxymethyl- pyrrolidine-5-carboxylic acid t-butyl ester.

13E. (~)-(2R,3R,5R,1'S)-2-(1-Acetamido-3-ethyl)pentyl-3-(oxazol-2 -yl)- nvrrolid ine-5-carboxylic Acid Dihvdrochloride.

The title compound is prepared according to the method described in Example 1K, substituting ()- (2R, 3R, 5R, 1'S)-2-(1-acetamido-3-ethyl)pentyl-3- (oxazol-2-yl)-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3R, 5R, 1'S)-2- (1-acetamido-3-ethyl) pentyl-3-methoxymethyl-pyrrolidine-5- carboxylic acid t-butyl ester.

Example14 <BR> <BR> <BR> (i)-(2S. 3R. 5R. 1'S)-2-(1-Acetamido-3-methvl) butvl-3-(N-methylamino) nvrrolidine- 5-carboxylic Acid Dihydrochloride.

14A. (i)-(2S, 3R. 5R)-1-Benzyl-2-vinvl-3-(N-methvl-N-benzyloxvcarbonVlamino)- pyrrolidine-5-carboxylic Acid t-Butyl Ester.

A solution of ()- (2S, 3R, 5R)-1-benzyl-2-vinyl-3-benzyloxycarbonylamino- pyrrolidine-5-carboxylic acid t-butyl ester (2.08 g, 4.77 mmole) was dissolved in 50 mL of anhydrous DMF and maintained under a nitrogen atmosphere. The solution was treated with sodium hydride (0.32 g, 8 mmole), and stirred at room temperature for 30 minutes. The solution was treated with iodomethane (0.8 ml, 12.85 mmole) and stirred for an additional 1 hour. The reaction was quenched with water and extracted with ethyl acetate. The combined organic layers were concentrated to provide the crude product which was purified by chromatography on silica gel to provide the title compound as an oil (yield : 1.75 g, 81 %).

'H NMR (CDC13) 6 7.36-7.20 (m, 10H), 5.75-5.50 (br, 1H), 5.25-5.07 (m, 4H), 4.75-4.50 (br, 1 H), 3.97 (d, J=13.5Hz, 1H), 3.75 (m, 1H), 3.61 (d, J=13. 5Hz, 1 H), 3.50 (m, 1 H), 2.93 (s, 3H), 2.45 (m, 1 H), 1.75 (m, 1 H), 1.46 (s, 9H).

MS (M+H) + = 451.

14B. (~)-(2R,3R,5R)-1-Benzyl-2-formyl-3-(N-methyl-N-benzyloxycarb onyl- amino) pvrrolidine-5-carboxylic Acid t-Butvl Ester.

The title compound was prepared according to the method described in Example 9C, substituting ()- (2S, 3R, 5R)-1-benzyl-2-vinyl-3-(N-methyl-N-benzyl- oxycarbonylamino) pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2S, 3R, 5R)-1-benzyl-2-vinyl-3-benzyloxycarbonylamino-pyrrolidine-5- carboxylic acid t-butyl ester. (Yield : 747 mg, 42%.) MS (M+H) + = 453.

14C. (t)-(2R, 3R. 5R)-1-Benzyl-2-(1-oxo-3-methyl) butyl-3-(N-methyl-N- benzyloxycarbonylamino) pyrrolidine-5-carboxylic Acid t-Butyl Ester.

Isobutyl magnesium chloride (2.0 M in ether, 0.68 ml) was added dropwise over about 12 minutes to a solution of ()- (2R, 3R, 5R)-1-benzyl-2-formyl-3-(N- methyl-N-benzyloxycarbonylamino) pyrrolidine-5-carboxylic acid t-butyl ester (196 mg, 0.43 mmole) in 5 mL of anhydrous THF, maintained at-78 °C. The resulting yellow solution was stirred at-78 °C for 1 hour. The solution was quenched with saturated aqueous ammonium chloride, and extracted with ethyl acetate. The organic layer was concentrated and the crude product was oxidized according to the procedure described in Example 9D. Purification by column chromatography on silica gel, with 10-25% ethyl acetate/hexanes, provided the title compound (yield : 78 mg, 36%).

1H NMR (CDC13) 8 7.46-7.25 (m, 10H), 5.09 (br, 2H), 4.90-4.60 (m, 1H), 3.97-3.65 (m, 4H), 3.00 (s, 3H), 2.60 (br, 1H), 2.20-1.80 (m, 3H), 1.46 (s, 9H), 0.80-0.67 (m, 7H).

MS (M+H) + = 509.

14D. (~)-(2S,3R,5R,1'R)- and (~)-(2S,3R,5R,1'S)-1-Benzyl-2-(1-amino-3- <BR> <BR> <BR> methyl) butN-methyl-N-benzoxycarbonvlamino) pyrrolidine-5-carboxylic Acid t-Butyl Ester.

The title compound was prepared according to the method described in Example 9F, substituting ()- (2R, 3R, 5R)-1-benzyl-2-(1-oxo-3-methyl)butyl-3-(N- methyl-N-benzyloxycarbonylamino) pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3R, 5R)-1-benzyl-2-formyl-3-benzyloxycarbonylamino-pyrrolidine- 5-carboxylic acid t-butyl ester. (Yield : 97 mg, 65%.) MS (M+H) + = 510.

14E. (i)-(2S, 3R. 5R. 1'R)- and (~)-(2S,3R,5R,1'S)-1-Benzyl-2-(1-acetamido-3- <BR> <BR> <BR> methyl)buyl-3- (N-methyl-N-benzyloxycarbonvlamino) pyrrolidine-5-carboxylic Acid t-Butyl Ester.

A solution of ()- (2S, 3R, 5R, 1'R)- and ()- (2S, 3R, 5R, 1'S)-1-benzyl-2- (1- amino-3-methyl) butyl-3- (N-methyl-N-benzyloxycarbonylamino) pyrrolidine-5- carboxylic acid t-butyl ester (47 mg, 0.094 mmole) was reacted with acetic anhydride (0.15 mL) in 4 mL of dichloromethane at room temperature for 2 hours.

The reaction was concentrated in vacuo to provide the title compound.

MS (M+H) + = 552.

14F. ()- (2S, 3R, 5R, 1'S)-2- (l-Acetamido-3-methyl) bulyl-3- (N-methylamino)- pyrrolidine-5-carboxylic Acid t-Butyl Ester.

A solution of (~)-(2S, 3R, 5R, 1'R)-and (i)-(2S, 3R, 5R, 1'S)-1-benzyl-2-(1- <BR> <BR> acetamido-3-methyl) butyl-3- (N-methyl-N-benzyloxycarbonylamino) pyrrolidine-5- carboxylic acid t-butyl ester (0.094 mmole), palladium (40 mg, 10% on carbon) and ammonium formate (160 mg) in 3 mL of ethanol was heated at reflux for 30 minutes. Additional palladium on carbon (15 mg) and ammonium formate (50 mg) were added. The solution was stirred for an additional 15 minutes and the mixture was then filtered to remove the solids and catalyst. The filtrate was evaporated and the residue purified by chromatography on silica gel 5% methanol/dichloromethane and 1% NH40H to provide ()- (2S, 3R, 5R, 1'S) (15.4 mg, lower Rfl and ()- (2S, 3R, 5R, 1'R) (5.4 mg, higher Rf)-2- (1-acetamido-3- methyl) butyl-3- (N-methylamino) pyrrolidine-5-carboxylic acid t-butyl esters (yield : 20.8 mg, 68%).

14G. (~)-(2S,3R,5R,1'S)-2-(1-Acetamido-3-methyl)butyl-3-(N-methyl amino)- pyrrolidine-5-carboxylic Acid Dihydrochloride.

A solution of ()- (2S, 3R, 5R, 1'S)-2-(1-Acetamido-3-methyl)butyl-3-(N- methylamino) pyrrolidine-5-carboxylic acid t-butyl ester (9.4 mg) was stirred with 4 N aqueous HC ! (-1. 5 mL) for 2 hours. The reaction was concentrated in vacuo to provide the title compound (yield : 10 mg, 100%).

1H NMR (major peaks) (DMSO-d6) 82. 57 (s, 3H), 1.90 (s, 3H), 1.47 (m, 3H), 0.91 (d, J=7.5Hz, 3H), 0.83 (d, J=7.5Hz, 3H) MS: (M+H) + = 272.

Example15 <BR> <BR> <BR> (i)-(2R. 3R. 5R. 1'S)-2-(1-Acetamido-3-ethyl) pentyl-3-(imidazol-2-yl)-pyrrolidine-5- Carboxylic Acid Dihydrochloride.

15A. (~)-(2R, 3R. 5R, 1'S)-1-Benzyl-2-(1-acetamido-3-ethyl) nentyl-3-(imidazol-2- vl)-pyrrolidine-5-carboxylic Acid t-Butyl Ester.

Ammonia gas was bubbled slowly through a solution (~)- (2R, 3R, 5R, 1'S)-1-benzyl-2-(1-acetamido-3-ethyl)pentyl-3-formyl-pyrroli dine-5- carboxylic acid t-butyl ester (20 mg, 0.045 mmole) and glyoxal (6.2 uL, 0.054 mmole, 1.2 equiv.) in 5 mL of methanol, maintained at 0 °C, for 5 minutes. After 7 hours at 0 °C, additional glyoxal (10 uL) was added and ammonia was bubbled through the solution for 5 minutes. The reaction was allowed to stir at room temperature for 16 hours. A final addition of glyoxal (10 uL) and ammonia as, described above, followed by reaction at room temperature for an additional 4 hours effected a complete reaction. The reaction was concentrated in vacuo and purified by chromatography on silica gel using 50% ethyl acetate/hexanes, followed by 10% methanol/chloroform to provide the title compound as a solid (yield : 19. 9 mg, 91%).

1 H NMR (CDC13) : d 0.67 (t, J=7.2 Hz, 3H), 0. 73 (t, J=7.2 Hz, 3H), 1.09- 1.32 (m, 7H), 1.41 (s, 9H), 2.00 (m, 1H), 2.09 (s, 3H), 2.79 (m, 1H), 3.29 (m, 1H), 3.66 (dd, J=9.6,2.7 Hz, 1H), 3.77 (m, 1H), 3.92 (d, J=13. 4 Hz, 1H), 4.04 (d, J=13.4 Hz, 1 H), 4.22 (dd, 1 H), 4.49 (m, 1 H), 6.08 (br s, 1 H), 7.00 (s, 2H), 7.21- 7.34 (m, 5H).

MS (M+H) + = 483.

15B. (i)-(2R, 3R, 5R, 1'S)-2-(1-Acetamido-3-ethyl) pentyl-3-(imidazol-2-yl)- pyrrolidine-5-carboxylic Acid t-Butyl Ester A mixture of ()- (2R, 3R, 5R, 1'S)-1-benzyl-2-(1-acetamido-3-ethyl)pentyl-3- (imidazol-2-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (17 mg, 0.035 mmole), ammonium formate (250 mg) and 10% palladium on carbon (20 mg), in 5 mL of ethanol, was heated at reflux for 15 minutes The reaction was concentrated in vacuo and the residue was purified by chromatography on silica gel using 5% methanol/dichlormethane and 0.25% ammonium hydroxide to provide the title compound as a white solid (yield : 11.3mg, 81.9%).

MS (M+H) 393.

15C. (~)-(2R,3R,5R,1'S)-2-(1-Acetamido-3-ethyl)pentyl-3-(imidazol -2-yl)- nvrrolidine-5-Carboxylic Acid Dihydrochloride.

()- (2R, 3R, 5R, 1'S)-2- (1-Acetamido-3-ethyl) pentyl-3- (imidazol-2-yl)- pyrrolidine-5-carboxylic acid t-butyl ester (11 mg, 0.028 mmole) was dissolved in 2 mL of 6N HCI and stirred at room temperature for 2 hours. The reaction was concentrated in vacuo to provide the title compound, as an off white solid (yield : 11.3 mg, 100%).

1 H NMR (DMSO-d6) : d 0.71 (t, J=7 Hz, 3H), 0.75 (t, J=7 Hz, 3H), 1.09- 1.28 (m, 7H), 1.74 (s, 3H), 2.43 (m, 1H), 2.80 (m, 1H), 3.85 (m, 1 H), 4.04 (m, 1 H), 4.29 (m, 1 H), 4.52 (m, 1 H), 7.64 (s, 2H), 8.07 (br d, J=9 Hz, 1 H).

MS (M+H) + = 337 and (M-H)-= 335.

Example 16 <BR> <BR> <BR> ()- (2R. 3R, 5R. 1'S)-2- (1-Acetamido-3-ethvl) pentyl-3- (N, N-dimethylcarbamov pyrrolidine-5-carboxvlic Acid Hydrochloride.

16A. (~)-(2R,3R,5R,1'S)-1-Benzyl-2-(1-acetamido-3-ethyl)pentyl-3- (N,N- dimethvicarbamoyl) pyrrolidine-5-carboxylic Acid t-Butyl Ester.

The title compound was prepared according to the method described in Example 5A substituting N, N-dimethylamine in place of N-methylamine (yield : 10 mg, 23%).

Mass spectrum: (M+H) + = 488. 16B. (~)-(2R,3R,5R,1'S)-2-(1-Acetamido-3-ethyl)pentyl-3-(N-methyl carbamoyl)- pyrrolidine-5-carboxylic-Acid t-Butvl Ester.

The title compound was prepared according to the method described in Example 1J, substituting ()- (2R, 3R, 5R, 1'S)-1-benzyl-2-(1-acetamido-3- ethyl) pentyl-3- (N, N-dimethylcarbamoyl) pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3R, 5R, 1'S)-1-benzyl-2-(1-acetamido-3-ethyl)pentyl-3-methoxy- methyl-pyrrolidine-5-carboxylic acid t-butyl ester to provide the title compound (yield : 5.5 mg, 67%).

Mass spectrum: (M+H) + = 398.

16C. (i)-(2R, 3R, 5R. 1'S)-2-(1-Acetamido-3-ethvl ! nentyl-3-(N, N-dimethvl- carbamov))pvrro)idine-5-carboxv)ic Acid Hydrochloride.

The title compound was prepared according to the method described in Example 1K, substituting ()- (2R, 3R, 5R, 1'S)-2-(1-acetamido-3-ethyl)pentyl-3- (N, N-dimethylcarbamoyl) pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3R, 5R, 1'S)-2-(1-acetamido-3-ethyl)pentyl-3-(methoxymethyl)-pyrroli dine- 5-carboxylic acid t-butyl ester.

'H NMR (major peaks) (D20) d 3.15 (s, 3H), 2.94 (s, 3H), 1.98 (s, 3H), 0.80 (m, 6H) MS (M+H) + = 342, (M-H)'= 340.

Example 17 <BR> <BR> <BR> (s)-(2R, 3R. 5R. 1'S)-2-(1-Acetamido-3-Ethyl) nentyl-3-cvano-pvrrolidine-5- carboxylic Acid Hydrochloride.

17A. () (2S. 3R, 5R)-1-Benzyl-2-vinyl-3-cyano-pvrrolidine-5-carboylic Acid t-Butv Ester.

A solution of ()- (2S, 3S, 5R)-1-benzyl-2-vinyl-3-formyl-pyrrolidine-5- carboxylic acid t-butyl ester (8: 1 ratio) (5g, 15.9 mmole) with hydroxylamine hydrochloride (1.28g, 18.5 mmole) and 10% aqueous potassium carbonate (8 mL) in 20 mL of methanol, according to the procedure described by Chelucci et al., Tetrahedron : Asymmetry 5: 1973 (1994) provided an the intermediate oxime product.

The crude oxime, prepared above, was reacted with 1,1'- carbonyldiimidazole (3.9,23.9 mmole) in 50 mL of dichloromethane for 3 hours, at room temperature. The reaction was concentrated in vacuo and chromatographed on silica gel with 2-10% ethyl acetate/hexanes to provide the title compound (yield : 2.5g, 50%).

MS (M+H) += 313 17B. ()- (2R, 3R. 5R)-1-Benzyl-2-formyl-3-cyano-pyrrolidine-5-carboxylic Acid t- Butyl Ester.

The title compound is prepared according to the method described in Example 1D, substituting () (2S, 3R, 5R)-1-benzyl-2-vinyl-3-cyano-pyrrolidine-5- carboxylic acid t-butyl ester in place of ()- (2S, 3R, 5R)-1-benzyl-2-vinyl-3-(t- butyidimethylsilyloxymethyl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 2.2 g, 80%).

MS (M+H) += 315 17C. (M2R.3R.5R)-1-Benzvt-2-(1-oxo-3-ethv!)Dentv!-3-cvano-Dvrro)i dine-5- carboxylic Acid t-Butyl Ester.

The title compound was prepared according to the method described in Example 1E, substituting () (2S, 3R, 5R)-1-benzyl-2-formyl-3-cyano-pyrrolidine-5- carboxylic acid t-butyl ester in place of (t)- (2R, 3R, 5R)-1-benzyl-2-formyl-3- (t butyidimethylsilyloxymethyl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield 0.4 g , 27%).

MS (M+H) += 399 17D. (~)-(2R 3R, 5R. 1'S)-1-Benzyl-2-(1-amino-3-ethyl)pentyl-3-cyano-pyrrolidine- 5-carboxylic Acid t-Butyl Ester.

The title compound was prepared according to the method described in Example 1F, substituting ()- (2R, 3R, 5R)-1-benzyl-2-(1-oxo-3-ethyl)pentyl-3- cyano-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3R, 5R)-1- benzyl-2-(1-oxo-3-ethyl)pentyl-3-(t-butyldimethylsilyloxymet hyl)-pyrrolidine-5- carboxylic acid t-butyl ester (yield 0.215 g, 50%).

MS (M+H) += 400 17E. (~)-(2R, 3R, 5R, 1'S)-1-Benzv!-2-f1-acetam)do-3-ethv))Dentvt-3-cvano- pyrrolidine-5-carboxylic Acid t-Butyl Ester.

The title compound is prepared according to the method described in Example 1G, substituting ()- (2R, 3R, 5R, 1'S)-1-benzyl-2-(1-amino-3-ethyl)pentyl- 3-cyano-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3R, 5R, 1'R)- and ()- (2R, 3R, 5R, 1'S)-1-benzyl-2- (1-amino-3-ethyl) pentyl-3- (t butyldimethylsilyloxymethyl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield 0.210 g, 90%).

'H NMR (CDC13) 8 7.25 (m, 5H), 5.08 (m, 1H), 4.40 (m, 1H), 4.15 (m, 1H), 3.78 (m, 1H), 3.48 (m, 1H), 2.93 (m, 1H), 2.32 (m, 1H), 2.12 (m, 1H), 2.02 (s, 3H), 1.52 (s, 9H), 1.35 (m, 7H), 0.85 (m, 6H) MS: (M+H)+ = 442.

17F. (~)-(2R,3R,5R,1'S)-2-(1-Acetamido-3-ethyl)pentyl-3-cyano-pyr rolidine-t- carboxy) Acid t-Butyl Ester.

The title compound was prepared according to the method described in Example 1J, substituting ()- (2R, 3R, 5R, 1'S)-1-benzyl-2-(1-acetamido-3- ethyl) pentyl-3-cyano-pyrrolidine-5-carboxylic acid t-butyl ester in place (~)- (2R, 3R, 5R, 1'S)-1-benzyl-2-(1-acetamido-3-ethyl) pentyl-3-methoxymethyl- pyrrolidine-5-carboxylic acid t-butyl ester.

'H NMR (CDC13) 8 5.35 (bs, 1H), 4.00 (m, 1H), 3.83 (m, 1H), 3.39 (m, 1H), 3.08 (m, 1H), 2.63 (m, 1H), 2.15 (m, 1H), 2.05 (s, 3H), 1.48 (s, 9H), 1.20-1.45 (m, 7H), 0.85 (m, 6H) MS : (M+H)+=352 17G. (i)-(2R, 3R, 5R. 1'S)-2-(1-Acetamido-3-ethyl) pentyl-3-cyano-pvrrolidine-5- carboxylic Acid Hydrochloride.

The title compound was prepared according to the method described in Example 1K, substituting ()- (2R, 3R, 5R, 1'S)-2-(1-acetamido-3-ethyl)pentyl-3- cyano-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3R, 5R, 1'S)-2- (1-acetamido-3-ethyl) pentyl-3- (methoxymethyl)-pyrrolidine-5-carboxylic acid t- butyl ester.

'H NMR (d6-DMSO) 8 9.12 (bs, 1H), 8.05 (m, 1H), 4.38 (m, 1H), 4.23 (m, 1 H), 3.88 (m, 1 H), 3.68 (m, 1 H), 3.00 (m, 1 H), 2.55 (m, 1 H), 2.05 (m, 1 H), 1.88 (s, 3H), 1.10-1.40 (m, 7H), 0.80 (m, 6H) MS: (M+H)+= 296, (M-H)-= 294, Example 18 <BR> <BR> <BR> ()- (2R. 3S. 5R. 1'S)-2- (l-Acetamido-3-ethyl) pentyl-3-ethyl-pyrrolidine-5-carboxylic AcidHydrochloride.

18A. (t)- (2R. 3S, 5R. 1'S)-1-Benzvl-2- (1-acetamido-3-ethyl) pentyl-3-vinyl- pyrrolidine-5-carboxylic Acid t-Butyl Ester.

To an ice-cold suspension of methyl triphenylphosphonium bromide (240 mg, 0.67 mmol) in 5 mL THF was added potassium t-butoxide (60 mg, 0.54 mmol) under nitrogen. The color changed immediately to bright yellow. After stirring at room temperature for 1 h, (~)-(2R, 3R, 5R, 1'S)-1-benzyl-2- (1-acetamido- 3-ethyl) pentyl-3-formyl-pyrrolidine-5-carboxylic acid t-butyl ester (100 mg, 0.225 mmol) in 5 mL THF was added and stirred at room temperature overnight.

Reaction was then quenched with saturated ammonium chloride and extracted with ethyl acetate to give the crude product which was purified by chromatography on silica gel using 30% ethyl acetate/hexanes to provide the title compound, as an oil (yield: 55 mg, 55%). aH NMR (CDC13) : # 7.45-7.20 (m, 5H), 5.94 (ddd, 1H), 5.24 (d, J=12Hz, 1 H), 4.98 (d, J=18Hz, 1H), 4.93 (d, J=10.5HZ, 1H), 4.37 (m, 1H), 4.06 (d, J=13. 5Hz, 1H), 3.80 (d, J=13.5Hz, 1H), 3.41 (dd, J=9 Hz, J=3Hz, 1H), 3.31 (q, J=13. 5Hz, 1H), 2.60 (m, 1H), 2.26 (m, 1H), 2.00 (s, 3H), 1.45 (s, 9H), 1.40-1.25 (m, 7H), 0.82 (m, 6H).

MS: (M+H) += 443 18B. ()- (2R. 3S. 5R. 1'S)-2-(1-acetamido-3-ethyl)pentyl-3-ethyl-pyrrolidine-5- carboxylic Acid t-but Ester.

The title compound was prepared according to the method described in Example 1J, substituting ()- (2R, 3S, 5R, 1'S)-1-benzyl-2-(1-acetamido-3- ethyl) pentyl-3-vinyl-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3R, 5R, 1'S)-1-benzyl-2-(1-acetamido-3-ethyl)pentyl-3-methoxymethyl- pyrrolidine-5-carboxylic acid t-butyl ester. rH NMR (CDC13) : 8 5.71 (br, 1H), 4.00 (br, 1 H), 3.68 (t, J=8Hz, 1H), 3.10 (m, 1 H), 2.38 (m, 1 H), 1.98 (s, 3H), 1.87 (m, 1 H), 1.47 (s, 9H), 1.55-1.20 (m, 10H), 0.93 (t, J=7.5Hz, 3H), 0.83 (m, 6H).

MS: (M+H) += 355 18C. (~)- (2R, 3S, 5R. 1'S)-2-(1-acetamido-3-ethyl) pentyl-3-ethyl-pyrrolidine-5- carboxylic Acid-Hydrochloride The title compound was prepared according to the method described in Example 1 K, substituting (~)-(2R,3S,5R,1'S)-2-(1-acetamido-3-ethyl)pentyl-3- ethyl-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3R, 5R, 1'S)-2- (1-acetamido-3-ethyl) pentyl-3- (methoxymethyl)-pyrrolidine-5-carboxylic acid t- butyl ester.

'H NMR (D20): 5 4.30 (br, 1 H), 4.25 (t, J=7.5Hz, 2H), 3.58 (br, 1 H), 2.61 (m, 1 H), 2.23 (br, 1H), 2.05 (s, 3H), 1.90 (m, 1 H), 1.70-1. 20 (m, 9H), 0.92 (t, J=7.5Hz, 3H), 0.81 (m, 6H).

MS: (M+H)+= 299 Example 19 -2R.3S.5R.1'S)-2-n-Acetamido-3-ethv))Dentvt-3-DroDv!-Dvrro)i dine-5- carboxylic Acid Hydrochloride.

19A. ()- (2R,3S,5R,1'S)-1-Benzyl-2-(1-acetamido-3-ethyl)pentyl-3-(cis -propen- 1-yl)-pyrrolidine-5-carboxvlic Acid t-Butyl Ester and (ffi)-(2R, 3S, 5R. 1'S)-1-Benzyl- <BR> <BR> <BR> 2- (1-acetamido-3-ethyl) pentyl-3- (trans-propen-1-yl)-pyrrolidine-5-carboxylic Acid<BR> <BR> <BR> <BR> t-Butyl Ester.

The title compound was prepared according the method described in Example 18A substituting ethyl triphenylphosphonium bromide for methyl triphenylphosphonium bromide.

1H NMR (CDCl3) # 7. 24 (m, 5H), 5.59 (m, 1H), 5.36 (dd, J= 11, 7Hz, 1H), 5.28 (bs, 1H), 4.32 (m, 1H), 4.06 (d, J= 12.9Hz, 1H), 3.80 (d, J= 12.9Hz, 1H), 3.42 (dd, J= 8.5,2. OHz, 1H), 3.30 (dd, J= 6.1,3. 1Hz, 1H), 2.88 (m, 1H), 2.29 (m, 2H), 2.01 (s, 3H), 1.64 (dd, J= 6.8,1.7Hz, 3H), 1.44 (s, 9H), 1.30 (m, 7H), 0.81 (m, 6H).

MS: (M+H) += 457, (M+Na) += 479, (M-H)-= 455.

19B. (iM2R.3S.5R.1'S-2-(1-acetamido-3-ethvnDentv)-3-DroDv)-Dvrro) idine-5- carboxylic Acid t-Butvl Ester.

The title compound was prepared according to the method described in Example 1J, substituting ()- (2R, 3S, 5R, 1'S)-1-benzyl-2-(1-acetamido-3- ethyl) pentyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (~)- (2R, 3S, 5R,1'S)-1-benzyl-2-(1-acetmaido-3-ethyl)pentyl-3-(trans-prop en-1-yl)- pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3R, 5R, 1'S)-1-benzyl- <BR> <BR> <BR> 2- (1-acetamido-3-ethyl) pentyl-3-methoxymethyl-pyrrolidine-5-carboxylic acid t- butyl ester (yield : 3.5 mg, 54%).

MS: (M+H) += 369, (M+Na)+= 391, (M-H)-= 367.

19C. ()- (2R. 3S. 5R. 1'S)-2- 1-acetamido-3-ethvl) pentvl-3-propvl-pvrrolidine-5- carboxylic Acid Hydrochloride The title compound was prepared according to the method described in Example 1K, substituting ()- (2R, 3S, 5R, 1'S)-2-(1-acetamido-3-ethyl)pentyl-3- ethyl-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3R, 5R, 1'S)-2- (1-acetamido-3-ethyl) pentyl-3- (methoxymethyl)-pyrrolidine-5-carboxylic acid t- butyl ester (yield : 3.5 mg, 100%).

'H NMR (DMSO-d6) 5 8.10 (d, J= 8.3Hz, 1H), 4.24 (m, 1H), 4.17 (m, 1H), 2.43 (m, 1H), 2.19 (m, 1H), 1.89 (s, 3H), 1.70 (m, 1H), 1.50-1.20 (m, 12H), 0.87 (t, J= 6.8Hz, 3H), 0.84 (t, J= 7. OHz, 3H), 0.79 (t, J= 7.3Hz, 3H).

MS: (M+H)+ = 313, (M+Na) + = 335, (M-H)-= 311.

Example 20 <BR> <BR> <BR> ft(2R, 3S, 5R, 1'S)-2- (1-Acetamido-3-methyl) butyl-3-vinvl-pyrrolidine-5-carboxylic Acid Hydrochloride.

20A. (~)-(2R, 3R, 5R. 1'RS)-1-Benzyl-2-(1,2-dihydroxy)ethyl-3-(t- butyldimethylsilyloxymethyl)-pyrrolidine-5-carboxylic Acid t-Bu ! Yl Ester.

Osmium tetroxide was added to a room temperature solution of ()- (2S, 3R, 5R)-1-benzyl-2-vinyl-3- (t-butyidimethylsilyloxymethyl)-pyrrolidine-5- carboxylic acid t-butyl ester (3.5 g, 8.12 mmol) in 60 mL of 8: 1 acetone/water and N-methylmorpholine N-oxide (3.0 g, 25.6 mmol). The reaction mixture was stirred at room temperature for 6 hours and quenched with saturated aqueous Na2S203.

The mixture was stirred for an additional 10 minutes and the solvent removed.

The brownish residue was partitioned between dichloromethane and water. The organic layer was dried over MgS04 and concentrated in vacuo to provide the intermediate diol as an oil (-3. 8g) which was used without additional purification.

MS: (M+H) + = 466.

20B. (~)-(2R,3R,5R,1'RS)-2-(1,2-Dihydroxy)ethyl-3-(t- butyldimethylsiloxymethvl)-pvrrolidine-5-carboxylic Acid t-Butyl Ester.

The title compound was prepared according to the method described in Example 1J, substituting ()- (2R, 3R, 5R, 1'RS)-1-benzyl-2-(1, 2-dihydroxy) ethyl-3- (t-butyldimethylsilyloxymethyl)-pyrrolidine-5-carboxylic acid t-butyl ester (21.5g, 46.2 mmol). in place of ()- (2R, 3R, 5R, 1'S)-1-benzyl-2-(1-acetamido-3- ethyl) pentyl-3-methoxymethyl-pyrrolidine-5-carboxylic acid t-butyl ester.

MS: (M+H) += 367.

20C. (~)-(2R,3R,5R,1'RS)-1-t-Butoxycarbonyl-2-(1,2-dihydroxy)-eth yl-3-(t- butyldimethylsilyloxymethyl)-pyrrolidine-5-carboxylic Acid t-Butyl Ester.

(~)-(2R,3R,5R,1'RS)-2-(1,2-Dihydroxy)ethyl-3-(t- butyldimethylsilyloxymethyl)-pyrrolidine-5-carboxylic acid t-butyl ester (crude from previous step) was dissolved in 160 mL of 3: 1 methanol/water and di-tert-butyl- dicarbonate (14.0 g, 64 mmol) was added. The mixture was stirred at room temperature for 72 h. Then solvent was removed and the residue was purified by chromatography on silica gel using 50% ethyl acetate/hexanes to provide the title compound as light yellow solid (yield : 15.4 g, 70 %). aH NMR (CDC13) : 8 0.03 (s, 3H), 0.05 (s, 3H), 1.37 (s, 9H),. 42 (s, 9H), 1.47 (s, 9H), 1.93 (d, 1 H), 2.30-2.50 (m, 2H), 3.28 (d, 1 H), 3.66-3.43 (m, 4H), 3.85 (dd, 1H), 4.02-4.52 (m, 1H).

MS: (M+H) += 476.

20D. (t)- (2R, 3R, 5R)-1-t Butoxycarbonvl-2-formyl-3- (t- butyldimethylsilyloxymethyl)-pyrrolidine-5-carboxylic Acid t-Butyl Ester.

A solution of ()- (2R, 3R, 5R, 1'RS)-1-t-butoxycarbonyl-2- (1, 2- dihydroxy) ethyl-3- (t-butyldimethylsilyloxymethyl)-pyrrolidine-5-carboxylic acid t- butyl ester (6.0 g, 12.6 mmol) was dissolved in 6: 1 tetrahydrofuran (THF)/water (110 mL) and treated with sodium periodate (4.4 g, 20.6 mmol). The mixture was stirred at room temperature for 3 hour and diluted with ethyl acetate, washed with water, dried over MgS04, filtered, and concentrated in vacuo. The residue was purified by chromatography on silica gel using 20% ethyl acetate/hexanes to provide the title compound as a white waxy solid (yield : 4.4 g, 78.6%).

'H NMR (CDC13) (mixture of two rotamers) : 8 0.05 and 0.06 (two s, 6H), 0.88 and 0.90 (two s, 9H), 1.42 and 1. 44 (two s,-9H), 1.47 and 1.48 (two s, 9H), 1.89-1.99 (m, 1 H), 2.37-2.43 (m, 2H), 3.54-3.67 (m, 2H), 4.02-4.34 (m, 2H), 9.43 and 9.53 (two d, 1H).

MS: (M+H) += 444.

20E. (~)-(2R,3R,5R,1'RS)-1-t-Butoxycarbonyl-2-(1-hydroxy-3-methyl )butyl-3-(t- butyldimethylsilyloxymethyl)-pyrrolidine-5-carboxylic Acid t-Buyl Ester.

A solution of ()- (2R, 3R, 5R)-1-t-butoxycarbonyl-2-formyl-3-(t- butyidimethylsilyloxymethyl)-pyrrolidine-5-carboxylic acid t-butyl ester (7.1 g, 16.03 mmol) in diethyl ether (75 mL) was reacted with isobutyl magnesium chloride (24 mL, 2.0 M in ether, 48 mmol) at 0°C for 2.5 hours. The reaction was quenched with saturated ammonium chloride and diluted with ethyl acetate. The organic layer was washed with water, and brine, dried over MgS04, filtered and concentrated in vacuo. The residue was used in next step without further purification.

MS: (M+H) += 502 20F. ()- (2R, 3R, 5R) 1-t-Butoxycarbonyl-2- butyl-3-(t- butidimethylsilvloxymethvl)-pyrrolidine-5-carboxylic Acid t-Butyl Ester.

A solution of oxalyl chloride (16 mL, 2M in CH2CIz) in 100 mL of anhydrous dichloromethane was prepared and maintained under a nitrogen atmosphere, at -78 °C. DMSO (4.26 mL, 64.1 mmol) was added slowly to the solution. The mixture was stirred for 15 minutes and reacted with ()- (2R, 3R, 5R, 1'RS)-1-t- butoxycarbonyl-2- (1-hydroxy-3-methyl) butyl-3- (t-butyldimethylsilyloxymethyl)- pyrrolidine-5-carboxylic acid t-butyl in 30 mL of anhydrous dichloromethane. The solution was stirred for 1 hour and triethylamine (17 mL, 128 mmol) was added slowly to the reaction mixture. The solution was allowed to warm slowly to room temperature, quenched with saturated sodium bicarbonate and diluted with dichloromethane. The organic layer was washed with water and brine, dried over MgS04, filtered and concentrated in vacuo. The residue was purified by chromatography on silica gel using 5-10% ethyl acetate/hexanes to provide the title compound (yield : 6.3 g, 78.8 %).

'H NMR (CDC13) : 6 0. 07 (m, 6H), 0.81-0.96 (m, 15H), 1.40 and 1.42 (two s, 9H), 1.46 and 1.47 (two s, 9H), 1.72-1.82 (m, 1H), 2.15-2.45 (m, 4H), 3.47- 3.69 (m, 1 H), 4.28-4.46 (m, 2H).

MS: (M+H) += 500 20G. (i)-(2 R. 3R. 5R, 1'RS)-1-t-Butoxycarbonyl-2-(1-amino-3-methy) butvi-3-(t- butyldimethylsilyloxymethyl)-pyrrolidine-5-carboxylic Acid t-Butyl Ester.

The title compound was prepared according to the method described in Example 1F, substituting ()- (2R, 3R, 5R) 1-t-butoxycarbonyl-2- (1-oxo-3- methyl) butyl-3- (t-butyldimethylsilyloxymethyl)-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3R, 5R)-1-benzyl-2-(1-oxo-3-ethyl)pentyl-3-(t-butyl- dimethylsilyloxymethyl)-pyrrolidine-5-carboxylic.. acid t-butyl ester (yield : 0.54 g, 34.1%).

MS : (M+H) += 501.

20H. (~)-(2R,3R,5R,1'S)-t-Butoxycarbonyl-2-(1-acetamido-3-methyl) butyl-3-(t- butyldimethylsilyloxymethyl)-pvrrolidine-5-carboxylic Acid t-Buyl Ester.

The title compound was prepared according to the method described in Example 1G, substituting ()- (2R, 3R, 5R, 1'RS)-1-t butoxycarbonyl-2- (1-amino-3- methy) butyl-3- (t-butyldimethylsilyloxymethyl)-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3R, 5R, 1'R)- and ()- (2R, 3R, 5R, 1'S)-1-benzyl-2-(1- amino-3-ethyl)pentyl-3-(t-butyldimethylsilyloxymethyl)-pyrro lidine-5-carboxylic acid t-butyl ester (yield : 462 mg, 79.0 %).

()- (2R, 3R, 5R, 1'S) 1H NMR (CDCl3) : 8 0.03 and 0.04 (two s, 6H), 0.86 (s, 9H), 0.89 and 0.95 (two d, 6H), 1.04 (m, 1H), 1.17-1.25 (m, 2H), 1.44 (s, 9H), 1.46 (s, 9H), 1.86 (m, 1 H), 1.99 (s, 3H), 2.07 (m, 1 H), 2.30 (m, 1 H), 3.48 (m, 1 H), 3.61 (m, 1H), 3.67 (m, 1H), 4.16 (m, 1H), 4.27 (m, 1 H), 7.35 (br d, 1 H).

MS: (M+H)+= 543.

20l. (#)-(2R,3R,5R,1'S)-1-t-Butoxycarbonyl-2-(1-acetamido-3-methy )butyl-3- (hydroxymethyl)-pyrrolidine-5-carboxylic Acid t-Butyl Ester.

The title compound was prepared according to the method described in Example 1H, substituting ()- (2R, 3R, 5R, 1'S)-1-t butoxycarbonyl-2- (1-acetamido- 3-methy) butyl-3- (t-butyldimethylsilyloxymethyl)-pyrrolidine-5-carboxylic acid t- butyl ester in place ()- (2R, 3R, 5R, 1'S)-1-benzyl-2- (1-acetamido-3-ethyl) butyl-3-t- butyldimethylsilyloxymethyl-pyrrolidine-5-carboxylic acid t-butyl ester.

MS: (M+H) += 429.

20J. (~)-(2R,3R,5R,1'S)-1-t-Butoxycarbonyl-2-(1-acetamido-3-methy )butyl-3- formyl-pyrrolidine-5-carboxylic Acid t-Butyl Ester.

The title compound was prepared according to the method described in Example 2A, substituting ()- (2R, 3R, 5R, 1'S)-1-t-butoxycarbonyl-2- (1-acetamido- 3-methy) butyl-3-hydroxymethyl-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3R, 5R, 1'S)-1-benzyl-2-(1-acetamido-3-ethyl)pentyl-3-hydroxymethyl- pyrrolidine-5-carboxylic acid t-butyl ester (yield : 1.5 g, 91 %).

1H NMR (CDC13) : 8 0.92 and 0.94 (two d, 6H), 1.07 (m, 1H), 1.23-1.33 (m, 2H), 1.43 (s, 9H), 1.44 (s, 9H), 1.64 (m, 1 H), 2.03 (s, 3H), 2.39 (m, 1H), 2.46 (m, 1H), 3.18 (m, 1H), 4. 19 (m, 1H), 4.32 (m, 1H), 4.39 (m, 1H), 7.12 (br d, 1H).

MS: (M+H) += 427 20K. (~)-(2R,3S,5R,1'S)-1-t-Butoxycarbonyl-2-(1-acetamido-3-methy )butyl-3- vinyl-pyrrolidine-5-carboxylic Acid t-Butyl Ester.

To a suspension of methyl triphenylphosphonium bromide (125.6 mg, 0.35 mmol) in 3 ml of anhydrous toluene was added potassium t-butoxide (1.0 M in THF, 0.31 mmol) dropwise at room temperature. After stirring for 16 hours, (i)- (2R, 3R, 5R, 1'S)-1-t-butoxycarbonyl-2- (1-acetamido-3-methyl) butyl-3-formyl- pyrrolidine-5-carboxylic acid t-butyl ester (30 mg, 0.070 mmol) in 3 ml of toluene was added dropwise and stirred for 0.5 hour. The reaction was quenched with saturated ammonium chloride and diluted with methylene chloride. The organic layer was washed with water and brine, dried over MgS04, filtered and concentrated in vacuo. The residue was purified by chromatography on silica gel using ethyl acetate to provide the title compound, as an white foamy solid (yield : 23.7 mg, 79.4%).

'H NMR (CDC13) : 5 0.92 (m, 6H), 1.26 (m, 2H), 1.44 (s, 9H), 1.47 (s, 9H), 1.65 (m, 1H), 1.97 (s, 3H), 2.43 (m, 2H), 3.56 (m, 1 H), 4.15 (m, 2H), 4.32 (m, 1H), 5.11 (m, 1 H), 5.15 (m, 1 H), 5.75 (m, 1H), 7.35 (br, 1H).

MS : (M+H) += 425.

20L. (~)- (2R, 3S. 5R. 1'S)-2- (1-Acetamido-3-methyl)butyl-3-vinyl-pyrrolidine-5- carboxylic Acid Hydrochloride.

The title compound was prepared according to the method described in Example 1K, substituting ()- (2R, 3S, 5R, 1'S)-1-t butoxycarbonyl-2- (1-acetamido- 3-methy) butyl-3-vinyl-pyrrolidine-5-carboxylic acid t-butyl ester in place of (~)- (2R, 3R, 5R, 1'S)-2-(1-acetamido-3-ethyl)pentyl-3-(methoxymethyl)-pyrroli dine-5- carboxylic acid t-butyl ester (yield : 16.0 mg, 99.1%).

'H NMR (DMSO-d6) : 8 0.82 (d, 3H), 0.88 (d, 3H), 1.29 (m, 1H), 1.42 (m, 1 H), 1.57 (m, 1 H), 1.87 (s, 3H), 1.91 (m, 1 H), 2.40 (m, 1 H), 2.90 (m, 1 H), 4.20 (m, 1H), 4.32 (m, 1H), 5.08 (dd, 1H), 5.17 (dd, 1H), 5.72 (ddd, 1H), 8.09 (d, 1H), 9.16 (br s, 1 H), 9.28 (br s, 1 H).

MS: (M+H)+= 269.

Example 21 (~)-(2R,3R,5R,1'S)-2-(1-Acetamido-3-ethyl)pentyl-3-hydroxyme thyl-pyrrolidine-5- carboxylic Acid Hydrochloride.

21A. (~)-(2R, 3R, 5R. 1'S)-2-(1-Acetamido-3-ethyl)pentyl-3-hydroxymethyl- pyrrolidine-5-carboxylic Acid t-Butyl Ester.

The title compound was prepared according to the method described in Example 1J, substituting ()- (2R, 3R, 5R, 1'S)-1-benzyl-2- (1-acetamido-3- ethyl) pentyl-3-hydroxymethyl-pyrrolidine-5-carboxylic acid t-butyl ester in place of (~)- (2R, 3R, 5R, 1'S)-1-benzyl-2- (1-acetamido-3-ethyl) pentyl-3-methoxymethyl- pyrrolidine-5-carboxylic acid t-butyl ester.

MS: (M+H) += 471 21B. (~)-(2R,3R,5R,1'S)-2-(1-Acetamido-3-ethyl)pentyl-3-hydroxyme thyl- pyrrolidine-5-carboxylic Acid Hydrochloride.

The title compound was prepared according to the method described in Example 1K, substituting ()- (2R, 3R, 5R, 1'S)-2-(1-acetamido-3-ethyl)pentyl-3- hydroxymethyl-pyrrolidine-5-carboxylic acid t-butyl ester in place of (t)- (2R, 3R, 5R, l'S)-2- (l-acetamido-3-ethyl) pentyl-3-methoxymethyl-pyrrolidine-5- carboxylic acid t-butyl ester 'H NMR (DMSO-d6) : 8 8.15 (d, J=9Hz, 1H), 4.28-4.15 (m, 2H), 3.95-3.45 (m, 4H), 2.35 (m, 1H), 1.98 (m, 1H), 1.89 (s, 3H), 1.50-1.45 (m, 7H), 0.81 (t, J=7.4Hz, 3H), 0.77 (t, J=7.5Hz, 3H).

MS: (M+H)+= 301, (M-H)-= 299 Example 22 <BR> <BR> <BR> <BR> <BR> (i)-(2R, 3R. 5R. 1'S)-2-(1-Acetamido-3-ethyl) pentvI-3-(pyrrol-1-yl)-pyrrolidine-5- carboxylic Acid Hvdrochloride.

22A. (~)-(2S,3R,5R,1'S)-1-Benzyl-2-(1-Acetamido-3-ethyl)pentyl-3- (2- trimethylsilylethoxycarbonylamino)-pyrrolidine-5-carboxylic Acid t-Butyl Ester.

()- (2R, 3R, 5R, 1'S)-1-Benzyl-2-(1-acetamido-3-ethyl)pentyl-3-carboxyl- pyrrolidine-5-carboxylic acid t-butyl ester (80 mg, 0.18 mmol) prepared according to the procedure of Example 2B was reacted with diphenylphosphoryl azide (0.047 mL, 0.216 mmol), 2-trimethylsilylethanol (0.034 mL, 0.234 mmol), and triethylamine (0.030 mL, 0.216 mmol) in toluene (2 mL) at 75°C for 15 hours. The reaction was concentrated in vacuo and the resulting residue purified by chromatography on silica gel using 25% ethyl acetate/hexanes to provide the title compound, as a light yellow oil (yield : 46 mg, 45%).

MS: (M+H) += 576, (M+Na) + = 598, (M-H)-= 574.

22B. ()- (2S. 3R, 5R. 1'S)-1-Benzyl-2- (1-acetamido-3-ethyl pentyl-3-amino- pyrrolidine-5-carboxvlic Acid t-Butyl Ester.

The title compound is prepared according to the method described in Example 1H, substituting ()- (2S, 3R, 5R, 1'S)-1-benzyl-2-(1-acetamido-3- ethyl) pentyl-3-(2-trimethylsilylethoxycarbonylamino)-pyrrolidine-5 -carboxylic acid t-butyl ester in place of ()- (2R, 3R, 5R, 1'S)-2-(1-acetamido-3-ethyl)pentyl-3-(t- butyldimethylsilyloxmethyl)-pyrrolidine-5-carboxylic acid t-butyl ester.

MS: (M+H) += 432, (M-H)-= 430. 22C. (~)-(2S,3R,5R,1'S)-1-Benzyl-2-(1-Acetamido-3-ethyl)pentyl-3- (pyrrol-1-yl)- pyrrolidine-5-carboxylic Acid t-Butyl Ester.

()- (2S, 3R, 5R, 1'S)-1-Benzyl-2-(1-acetamido-3-ethyl)pentyl-3-amino-. pyrrolidine-5-carboxylic-acid t-butyl ester (34 mg, 0. 078 mmol) was reacted with 40% succinic dialdehyde in water (50 mg, 0.234 mmol), acetic acid (0.00044 mL, 0.0078 mmol), and 4A molecular sieves (200 mg) in toluene (2 mL) at RT for 3 hours. The reaction was concentrated in vacuo and the resulting residue purified by chromatography on silica gel using 50% ethyl acetate/hexanes to provide the title compound, as an oil (yield : 7.1 mg, 19%).

MS: (M+H) += 482, (M+Na) + = 504, (M-H)-= 480.

22D. (~)-(2S,3R,5R,1'S)-2-(1-Acetamido-3-ethyl)pentyl-3-(pyrrol-1 -yl)- nyrrolidine-5-carboxylic Acid t-Butvl ester.

The title compound is prepared according to the method described in Example 1J, substituting ()- (2S, 3R, 5R, 1'S)-1-benzyl-2-(1-acetamido-3- ethyl) pentyl-3-(pyrrol-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3R, 5R, 1'S)-1-benzyl-2-(1-acetamido-3-ethyl) pentyl-3-methoxymethyl- pyrrolidine-5-carboxylic acid t-butyl ester (yield : 3.5 mg, 61%).

MS: (M+H) += 392, (M-H)-= 390.

22E. ()- (2S, 3R, 5R, 1'S)-2- (1-Acetamido-3-ethyl pentypvrrol-1-yl<BR> <BR> <BR> <BR> <BR> pyrrolidine-5-carboxylic Acid Hydrochloride.

The title compound was prepared according to the method described in Example 1K, substituting ()- (2S, 3R, 5R, 1'S)-2-(1-acetamido-3-ethyl)pentyl-3- (pyrrol-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester in place of (t)- (2R, 3R, 5R, 1'S)-2- (1-acetamido-3-ethyl) pentyl-3-methoxymethyl-pyrrolidine-5- carboxylic acid t-butyl ester (yield : 3.5 mg, 100%).

1H NMR (D20) 8 7.48 (bs, 1H), 6.77 (bs, 2H), 5.97 (bs, 2H), 4.33 (m, 1H), 3.70 (m, 1H), 3.07 (m, 1H), 2.43 (m, 1H), 1.92 (m, 1H), 1.75 (s, 3H), 1.55 (m, 1H), 1.35-1.10 (m, 7H), 0.81 (m, 3H), 0.75 (m, 3H).

MS: (M+H) += 336, (M-H)-= 334.

Example 23 <BR> <BR> <BR> ()- (2R, 3R, 5R. 1'S)-2- 1-Acetamido-3-ethvl) pentyl-3-i ; 1-cis-N-hvd roxyimino) eth pyrrolidine-5-carboxvlic Acid Hydrochloride.

23A. (~)-(2R,3R,5R,1'S)-1-Benzyl-2-(1-acetamido-3-ethyl)pentyl-3- (1-cis-N- hyd roxyimino) ethyl-pyrrolidine-5-carboxylic Acid t-Butyl Ester.

()- (2R, 3R, 5R, 1'S) 1-Benzyl-2- (1-acetamido-3-ethyl) pentyl-3-acetyl- pyrrolidine-5-carboxylic acid t-butyl ester (45 mg, 0.1 mmol) prepared according to the method of Example 8B in methanol/methylene chloride (3/1) was reacted with a solution of hydroxylamine hydrochloride (21 mg, 0.3 mmol) and sodium hydroxide (12 mg, 0.3 mmol) in methanol (2 mL) for 2h. The reaction was diluted with ethyl acetate. The organic layer was washed with water, and brine, dried over MgS04, filtered and concentrated in vacuo. The residue was purified by chromatography on silica gel using 40% ethyl acetate/hexanes to provide the cis- oxime title compound (lower Rf spot on TLC), as an oil (yield : 35 mg, 75%), as well as the trans-oxime title compound (higher Rf spot on TLC), as an oil (yield : 13 mg, 25%).

MS : (M+H) += 474 23B (~)-(2R,3R,5R,1'S)-2-(1-acetamido-3-ethyl)pentyl-3-(1-cis-N- hydroxyimino)ethyl pyrrolidine-5-carboxylic Acid t-Butyl Ester.

The title compound is prepared according to the method described in Example 1J, substituting ()- (2R, 3R, 5R, 1'S)-1-benzyl-2-(1-acetamido-3- ethyl) pentyl-3- (1-cis-N-hydroxyimino) ethyl-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3R, 5R, 1'S)-1-benzyl-2-(1-acetamido-3-ethyl)pentyl-3- methoxymethyl-pyrrolidine-5-carboxylic acid t-butyl ester.

H NMR (CDC13) : 8 3.92 (br, 1H), 3.70 (m, 2H), 2.82 (m, 1H), 2.38 (m, 1H), 1.88 (s, 3H), 1.78 (s, 3H), 1.39 (s, 9H), 1.40-1.20 (m, 7H), 0.76 (m, 6H).

MS: (M+H)+= 384 23C. (~)-(2R,3R,5R,1'S)-2-(1-acetamido-3-ethyl)pentyl-3-(1-cis-N- hydroxvimino) ethvl-pyrrolidine-5-carboxylic Acid Hydrochloride The title compound is prepared according to the method described in Example 1K, substituting ()- (2R, 3R, 5R, 1'S)-2-(1-acetamido-3-ethyl)pentyl-3-(1- cis-N-hydroxyimino) ethyl-5-carboxylic acid t-butyl ester in place of (~)- (2R, 3R, 5R, 1'S)-2- (1-acetamido-3-ethyl) pentyl-3- (methoxymethyl)-pyrrolidine-5- carboxylic acid t-butyl ester.

1H NMR (D20): 54. 35 (m, 1H), 4.00 (m, 1H), 3.80 (m, 1H), 3.71 (m, 1H), 3.63 (m, 1H), 3.13 (q, J=8. 4Hz,, 1H), 2.64 (m, 1H), 2.18 (m, 1H), 1.97 (s, 3H), 1.85 (s, 3H), 1.50-1.10 (m, 7H), 0.77 (m, 6H).

MS: (M+H) += 328 Example 24 <BR> <BR> <BR> <BR> (t)- (2R, 3R. 5R, 1'S)-2- 1-Acetamido-3-ethyl) pentvl-3- (N-hydroxvimino) methyl- pyrrolidine-5-carboxylic Acid Hydrochloride.

24A. (~)-(2R,3R,5R,1'S)-2-(1-Acetamido-3-ethyl)pentyl-3-(N- hvdroxvimino) yrrolidine-5-carboxylic Acid t-Butyl Ester.

(~)-(2R,3R,5R,1'S)-1-Benzyl-2-(1-acetamido-3-ethyl)pentyl-3- formyl- pyrrolidine-5-carboxylic acid t-butyl ester (18 mg, 0.051 mmol) prepared according to the method of Example 2A was reacted with hydroxylamine hydrochloride (7 mg, 0.11 mmol) in 1 N NaOH in methanol (3 mL) at 25°C for 1.5 hours. The reaction was quenched with aqueous ammonium chlororide (3ml) and water (3ml) and taken by dichloromethane (2x 10 ml). The organic layer was washed with water, and brine, dried over MgSO4, filtered and concentrated in vacuo. The residue was purified by chromatography on silica gel using 5% methanol in dichloromethane to provide the title compound, as an oil (yield : 6 mg, 32%).

MS: (M+H)+=370 24B (~)-(2R,3R,5R,1'S)-2-(1-Acetamido-3-ethyl)pentyl-3-(N- hydroxvimino) methyl-pyrrolidine-5-carboxylic Acid Hydrochloride The title compound is prepared according to the method described in Example 1K, substituting ()- (2R, 3R, 5R, 1'S)-2-(1-acetamido-3-ethyl)pentyl-3-(N- hydroxyimino) methyl-5-carboxylic acid t-butyl ester in place of ()- (2R, 3R, 5R, 1'S)-2- (1-acetamido-3-ethyl) pentyl-3- (methoxymethyl)-pyrrolidine-5- carboxylic acid t-butyl ester.

Example 25 (~)-(2R,3R,5R,1'S)-2-(1-Acetamido-3-ethyl)pentyl-3-(methoxyi mino)methyl- pyrrolidine-5-carboxylic Acid.

25A. () - (2R. 3R. 5R. 1'S) 1-Benzyl-2-(1-acetamido-3-ethyl)pentyl-3- (methoxyimino) methyl-pyrrolidine-5-carboxylic Acid ()- (2R, 3R, 5R, 1'S) 1-Benzyl-2- (1-acetamido-3-ethyl) pentyl-3-cyano- pyrrolidine-5-carboxylic acid t-butyl ester (20 mg, 0.045 mmol) prepared according to the method of Example 17E was reacted with hydrogen chloride (0.45 mmol) in ether (2 mL) and methanol (0.1 mL) at 0°C for 5 hours. The reaction was neutralized with aqueous ammonium hydroxide and purified on silica gel with 3% methanol in dichloromethane to provide the title compound, as a white solid (yield : 5 mg, 26%).

MS: (M+H) +=418 25B. (i)-(2R. 3R. 5R. 1'S)-2-(1-Acetamido-3-ethyl) pentVl-3- (methoxyimino) methyl-pyrrolidine-5-carboxylic Acid.

The title compound is prepared according to the method described in Example 1J, substituting ()- (2R, 3R, 5R, 1'S) 1-Benzyl-2- (1-acetamido-3- ethyl) pentyl-3- (imino-methoxymethyl)-pyrrolidine-5-carboxylic acid t-butyl ester, in place of ()- (2R, 3R, 5R, 1'S)-1-benzyl-2- (1-acetamido-3-ethyl) pentyl-3- methoxymethyl-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 3.9 mg, 96%).

'H NMR (DMSO-d6) 6 7.52 (d, J=8.7 HZ, 1H), 7.15 (s, 1H), 6.77 (s, 1H), 3.68 (m, 1 H), 3.61 (s, 3H), 3.22 (m, 1 H), 2.51 (m, 1 H), 2.23 (m, 1 H), 1.82 (m, 1 H), 1.78 (s, 3H), 1.40 (m, 1H), 1.26 (m, 3H), 1.13 (m, 3H), 0.78 (t, J=6.5HZ, 3H), 0.72 (t, J=6.5HZ, 3H) MS: (M+H)+=328 Example 26 (~)-(2R, 3R. 5R, 1'S)-2-(1-Acetamido-3-methyl)butyl-3-(hydroxyacethyl)-pyrrol idine- 5-carboxylic Acid Hydrochloride.

26A. (~)-(2R,3R,5R, 1'S. 1"RS)-1-t-Butoxycarbonyl-2-(1-acetamido-3- methy) butyl-3- (1. 2-dihydroxy ethyl-pyrrolidine-5-carboxylic Acid t-Butyl Ester.

The title compound is prepared according to the method described in Example 20A substituting ()- (2R, 3R, 5R, 1'S)-1-t-butoxycarbonyl-2-(1-acetamido- 3-methy) butyl-3-vinyl-pyrrolidine-5-carboxylic acid t-butyl ester for ()- (2S, 3R, 5R)- 1-benzyl-2-vinyl-3- (t-butyldimethylsilyloxymethyl)-pyrrolidine-5-carboxylic acid t-butyl ester.

26B. (~)-(2R,3R,5R,1'S)-1-t-Butoxycarbonyl-2-(1-acetamido-3-methy l)butyl-3- (hydroxyacethyl)-pyrrolidine-5-carboxylic Acid t-Butyl Ester.

()- (2R, 3R, 5R, 1'S, 1"RS)-l-t-Butoxycarbonyl-2- (l-acetamido-3- methy) butyl-3-(-1, 2-dihydroxy) ethyl-pyrrolidine-5-carboxylic acid t-butyl ester is reacted with dibutyltin oxide in methanol according to the procedure of Kong in J.

Carbohydrate Chem. 1993, p. 557. The reaction is concentrated and the residue is redissolved in dichloromethane and reacted with bromine as described in the above reference to give the title compound.

26C. (~)-(2R,3R,5R,1'S)-2-(1-Acetamido-3-methy)butyl-3-(hydroxyac etyl)- pyrrolidine-5-carboxylic Acid Hydrochloride.

The title compound is prepared according to the method described in Example 1K, substituting ()- (2R, 3R, 5R, 1'S)-1-t-butoxycarbonyl-2-(1-acetamido- 3-methy) butyl-3-hydroxyacetyl-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3R, 5R, 1'S)-2-(1-acetamido-3-ethyl)pentyl-3-(methoxymethyl)- pyrrolidine-5-carboxylic acid t-butyl ester.

Example 27 (~)-(2S,3R,5R,1'S)-2-(1-Acetamido-3-methyl)butyl-3-amino-pyr rolidine-5- carboxylic Acid Dihydrochloride.

27A. (~)-(2S, 3R. 5R. 1'RS)-1-Benzyl-2-(1-hydroxy-3-methyl)butyl-3- benzyloxycarbonylamino-pyrrolidine-5-carboxylic Acid t-Butyl Ester.

The title compound was prepared according to the method described in Example 9D, substituting isobutylmagnesium bromide in place of 3- pentylmagnesium bromide.

27B. ()- (2S. 3R. 5R, 1'S)-1-Benzyl-2- (1-acetamido-3-methvl) butyl-3- benzyloxycarbonylamino-pyrrolidine-5-carboxylic Acid t-Butyl Ester.

The title compound was prepared according to the method described in Examples 9E-H substituting ()- (2R, 3R, 5R, 1'RS)-1-benzyl-2- (1-hydroxy-3- methyl) butyl-3-benzyloxycarbonylamino-pyrrolidine-5-carboxylic acid t-butyl ester for ()- (2R, 3R, 5R, 1'RS)-1-benzyl-2-(1-hydroxy-3-ethyl)pentyl-3- benzyloxycarbonylamino-pyrrolidine-5-carboxylic acid t-butyl ester as the starting material of the sequence in Example 9E.

27C. (~)-(2S, 3R. 5R. 1'S)-2-(1-acetamido-3-methyl)butyl-3-amino-pyrrolidine-5- carboxylic Acid Dihydrochloride The title compound was prepared according to the method described in Example 1K, substituting ()- (2R, 3R, 5R, 1'S)-2-(1-acetamido-3-methyl)butyl-3- amino-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3R, 5R, 1'S)-2- <BR> <BR> <BR> (1-acetamido-3-ethyl) pentyl-3- (methoxymethyl)-pyrrolidine-5-carboxylic acid t- butyl ester.

1H NMR (d6-DMSO) # 8.64 (bs, 1H), 8.32 (bs, 1H), 8.23 (bs, 1H), 8. 18 (d, J=6Hz, 1 H), 4.79 (d, J=7Hz, 1H), 4.42 (m, 1H), 4.33 (m, 1H, 4.21 (m, 1H), 4.07 (m, 1 H), 3.76 (m, 2H), 2.73 (m, 2H), 1.92 (m, 3H), 0.80-0.97 (m, 7H).

MS (M+H) + = 258 Example 28 (~)-(2R,3R,5R,1'S)-2-(1-Acetamido-3-methyl)butyl-3-methoxyca rbonyl- pyrrolidine-5-carboxylic Acid Hydrochloride.

28A. (~)-(2R,3R,5R,1'S)-1-t-Butoxycarbonyl-2-(1-acetamido-3-methy l)butyl-3- carboxyl-pyrrolidine-5-carboxvlic Acid t-Buyl Ester.

The title compound was prepared according to the method described in Example 2B, substituting ()- (2R, 3R, 5R, 1'S)-1-t-butoxycarbonyl-2-(1-acetamido- 3-methyl) butyl-3-formyl-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3R, 5R, 1'S)-1-benzyl-2-(1-acetamido-3-ethyl) pentyl-3-formyl-pyrrolidine-5- carboxylic acid t-butyl ester.

28B. ()- (2R. 3R, 5R, 1'S)-1-t-Butoxycarbonyl-2-(1-acetamido-3-methyl)butyl-3- methoxycarbonyl-pyrrolidine-5-carboxylic Acid t-Butyl Ester The title compound was prepared according to the method described in Example 2C, substituting ()- (2R, 3R, 5R, 1'S)-1-t-butoxycarbonyl-2-(1-acetamido- 3-methyl) butyl-3-carboxyl-pyrrolidine-5-carboxylic acid t-butyl ester in place of (~)- (2R, 3R, 5R, 1'S)-1-benzyl-2- (1-acetamido-3-ethyl) pentyl-3-carboxyl-pyrrolidine-5- carboxylic acid t-butyl ester.

28C. (~)-(2R,3R,5R,1'S)-2-(1-Acetamido-3-methyl)butyl-3-methoxyca rbonyl- pyrrolidine-5-carboxylic Acid Hvdrochloride.

The title compound was prepared according to the method described in Example 2E, substituting ()- (2R, 3R, 5R, 1'S)-1-t-butoxycarbonyl-2-(1-acetamido- 3-methyl) butyl-3-methoxycarbonyl-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3R, 5R, 1'S)-2-(1-acetamido-3-ethyl)pentyl-3-methoxycarbonyl- pyrrolidine-5-carboxylic acid t-butyl ester.

'H NMR (DMSO-d6) : 8 8.24,8.08 (d, J=9Hz, 1H), 4.44,4.36 (m, 1H), 4.25, 4.15 (m, 1H), 3.98,3.88 (m, 1H), 3.65,3.64 (s, 3H), 3.18,3.10 (m, 1H), 2.57,2.20 (m, 2H), 1.87,1.83 (s, 3H), 1.57 (m, 2H), 1.36 (m, 1H), 0.88 (d, J=7.5Hz, 3H), 0.82 (d, J=7.5Hz, 3H).

MS: (M+H) += 301 Example 29 (i)-(2R. 3R. 5R. 1'S)-2-(1-Acetamido-3-methvl) butvl-3-(imidazol-2-vl)-pyrrolidine-5- Carboxylic Acid Dihydrochloride. 29A. (~)-(2R,3R,5R,1'S)-1-Benzyl-2-(1-acetamido-3-methyl)butyl-3- (imidazol-2- yl)-pyrrolidine-5-carboxylic Acid t-Butyl Ester.

The title compound was prepared according to the method described in Example 15A, substituting ()- (2R, 3R, 5R, 1'S)-1-benzyl-2-(1-acetamido-3- methyl) butyl-3-formyl-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3R, 5R, 1'S)-1-benzyl-2- (1-acetamido-3-ethyl) pentyl-3-formyl-pyrrolidine-5- carboxylic acid t-butyl ester (yield : 27.4 mg, 83. %).

MS : (M+H)+=455.

29B. (~)-(2R,3R,5R,1'S)-2-(1-Acetamido-3-methyl)butyl-3-(imidazol -2-yl)- pyrrolidine-5-carboxylic Acid t-Butyl Ester.

The title compound was prepared according to the method described in Example 15B, substituting ()- (2R, 3R, 5R, 1'S)-1-benzyl-2-(1-acetamido-3- methyl) butyl-3- (imidazol-2-yl)-pyrrolidine-5-carboxylic acid t-butyl ester in place of (~)-(2R, 3R, 5R, 1'S)-1-benzyl-2- (1-acetamido-3-ethyl) pentyl-3- (imidazol-2-yl)- pyrrolidine-5-carboxylic acid t-butyl ester (yield : 19.1 mg, 95.5%).

MS: (M+H) += 365.

29C (i)-(2R, 3R. 5R. 1'S)-2-(1-Acetamido-3-methyl)butyl-3-(imidazol-2-yl)- pvrrolidine-5-Carboxylic Acid Dihydrochloride.

The title compound was prepared according to the method described in Example 15B, substituting ()- (2R, 3R, 5R, 1'S)-1-benzyl-2-(1-acetamido-3- methyl) butyl-3-(imidazol-2-yl)-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3R, 5R, 1'S)-1-benzyl-2-(1-acetamido-3-ethyl)pentyl-3-(imidazol-2-yl )- pyrrolidine-5-carboxylic acid t-butyl ester.

'H NMR (DMSO-d6) : 8 0.76 (d, J = 6.6 Hz, 3H), 0.82 (d, J = 6.6 Hz, 3H), 1.18 (t, 2H), 1.44 (m, 1H), 1.71 (s, 3H), 2.43-2.47 (m, 1H), 2.80 (m, 1H), 3.83 (m, 1 H), 4.05 (m, 1 H), 4.28 (m, 1 H), 4.55 (t, 1 H), 7.65 (s, 2H), 8.03 (d, J = 8.4 Hz, 1H).

MS: (M+H) += 326.

Example 30 <BR> <BR> <BR> ((2R, 3R. 5R, 1'S)-2- 1-Acetamido-3-methyl) butyl-3-(imidazol-4-yl)-pvrrolidine-5- carboxylic Acid Dihydrochloride.

30A. (~)-(2R,3R,5R,1'S)-1-t-Butoxycarbonyl-2-(1-acetamido-3-methy l)butyl-3- carboxyl-yvrrolidine-5-carboxylic Acid t-Butyl Ester.

The title compound was prepared according to the method described in Example 2B, substituting ()- (2R, 3R, 5R, 1'S)-1-t-butoxycarbonyl-2-(1-acetamido- 3-methy) butyl-3-formyl-pyrrolidine-5-carboxylic acid t-butyl ester prepared according to the method described in Example 20J in place ()- (2R, 3R, 5R, 1'S)-1- benzyl-2-(1-acetamido-3-ethyl)pentyl-3-formyl-pyrrolidine-5- carboxylic acid t-butyl ester (yield : 129.5 mg, >100 %).

MS : (M+H) += 443. 30B. (i)-(2R, 3R. 5R. 1'S)-1-Benzvl-2- (1-acetamido-3-methyl) butyl-3-diazoacetyl- pyrrolidine-5-carboxylic Acid t-Butyl Ester.

The title compound was prepared according to the method described in Example 12A, substituting ()- (2R, 3R, 5R, 1'S)-1-benzyl-2-(1-acetamido-3- methyl) butyl-3-carboxyl-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3R, 5R, 1'S)-1-benzyl-2-(1-acetamido-3-ethyl) pentyl-3-carboxyl-pyrrolidine-5- carboxylic acid t-butyl ester (yield : 218.8 mg, 100%).

MS: (M+H)+= 458.

30C. (~)-(2R, 3R. 5R. 1'S)-1-Benzvl-2- (1-acetamido-3-methvl) butyl-3- bromoacetyl-pyrrolidine-5-carboxylic Acid t-Butyl Ester.

The title compound was prepared according to the method described in Example 12B, substituting ()- (2R, 3R, 5R, 1'S)-1-benzyl-2-(1-acetamido-3- methyl) butyl-3-diazoacetyl-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3R, 5R, 1'S)-1-benzyl-2-(1-acetamido-3-ethyl)pentyl-3-diazoacetyl- pyrrolidine-5-carboxylic acid t-butyl ester (yield : 107.2 mg, %).

'H NMR (CDC13) : 6 0.90 (d, 6H), 1.26-1.35 (m, 3H), 1.42 (s, 9H), 1.95 (s, 3H), 2.25 (m, 2H), 3.11 (m, 1 H), 3.54 (dd, 1 H), 3.69 (m, 1 H), 3.93 (dd, 2H), 4.11 (d, 1 H), 4.27 (m, 1H), 4.35 (d, 1 H), 5.05 (br d, 1 H), 7.25-7.32 (m, 5H).

MS: (M+H)+=509. 30D. (i)-(2R. 3R. 5R. 1'S)-1-Benzvl-2-(1-acetamido-3-methyl) butyl-3-(imidazol4- yl)-pyrrolidine-5-carboxylic Acid t-Butyl-Ester.

The title compound was prepared according to the method described in Example 12C, substituting ()- (2R, 3R, 5R, 1'S)-1-benzyl-2-(1-acetamido-3- methyl) butyl-3-bromoacetyl-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3R, 5R, 1'S)-1-benzyl-2-(1-acetamido-3-ethyl)pentyl-3-bromoacetyl- pyrrolidine-5-carboxylic acid t-butyl ester (yield : 32.3 mg, 60.4%).

MS: (M+H)+= 455.

30E. (~)-(2R,3R,5R,1'S)-2-(1-Acetamido-3-methyl)butyl-3-(imidazol -4-yl)- pyrrolidine-5-carboxylic Acid t-Butyl Ester.

The title compound was prepared according to the method described in Example 1J, substituting (t)- (2R, 3R, 5R, 1'S)-1-benzyl-2-(1-acetamido-3- methyl) butyl-3- (imidazol-4-yl)-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3R, 5R, 1'S)-1-benzyl-2-(1-acetamido-3-ethyl)pentyl-3-methoxymethyl- pyrrolidine-5-carboxylic acid t-butyl ester (yield : 23.9 mg, 96.2%).

'H NMR (CDCl3) : 8 0. 87 (d, 3H), 0.89 (d, 3H), 1.26 (m, 1H), 1.41 (m, 2H), 1.46 (s, 9H), 1.59 (m, 1H), 1.93 (s, 3H), 2.62 (m, 1H), 3.30 (m, 1H), 3.54 (m, 1H), 3.79 (m, 1H), 4.01 (m, 1H), 6.11 (br d, 1H), 6.89 (s, 1H), 7.63 (s, 1H).

MS: (M+H)+= 365.

30F. (~)- (2R. 3R. 5R. 1'S)-2-(1-Acetamido-3-methyl)butyl-3-(imidazol-4-yl)- pyrrolidine-5-carboxylic Acid Dihydrochloride.

The title compound was prepared according to the method described in Example 1K, substituting ()- (2R, 3R, 5R, 1'S)-2-(1-acetamido-3-methyl)butyl-3- (imidazol-4-yl)-pyrrolidine-5-carboxylic acid t-butyl ester in place of (~)- (2R, 3R, 5R, 1'S)-2-(1-acetamido-3-ethyl)pentyl-3-(methoxymethyl)-pyrroli dine-5- carboxylic acid t-butyl ester to provide the title compound solid (yield : 24.4mg, 100%).

1H NMR (DMSO-d6) : # 0.76 (d, J = 3.6 Hz, 3H), 0.88 (d, J = 3.6 Hz, 3H), 1.22 (m, 1H), 1.28 (m, 1H), 1.48 (m, 1H), 1.79 (s, 3H), 2.32 (dt, 1H), 2.71 (dt, 1H), 3.68 (m, 1H), 3.96 (m, 1H), 4.28 (m, 1H), 4.51 (t, 1H), 7.63 (s, 1H), 8.23 (d, J = 5.1 Hz, 1H), 9.10 (s, 1H), 9.67 (br s, 1H), 14.51 (br s, 1H).

MS: (M+H) += 309.

Example 31 <BR> <BR> <BR> (i)-(2R, 3R. 5R. 1'S)-2-(1-Acetamido-3-methyl) butyl-3-(thiazol4-yl)-pyrrolidine-5- carboxylic Acid Dihydrochloride.

31 A. (i) -(2R. 3R. 5R. 1'S)-1-t-Butoxycarbonyl-2-(1-acetamido-3-methyl)butyl-3- (thiazol-4-yl)-pyrrolidine-5-carboxylic Acid t-Butyl Ester.

()- (2R, 3R, 5R, 1'S)-1-t-Butoxycarbonyl-2- (1-acetamido-3-methyl) butyl-3- bromoacetyl-pyrrolidine-5-carboxylic acid t-butyl ester (36.5 mg, 0.07 mmol) was reacted with thioformamide (21.4 mg, 0.35 mmol) in ethanol (5 ml) at reflux for 4 hours. The reaction was concentrated in vacuo. The residue was treated with 5 ml of aqueous NaHCO3 and extracted with dichloromethane (4 x 5 ml). The organic layers were washed with brine, dried over Na2CO4, filtered and concentrated in vacuo. The residue was purified by chromatography on silica gel using ethyl acetate to provide the title compound, as a white solid (yield : 23.8 mg, 70.4%).

MS: (M+H) += 482.

31B) (~)-(2R,3R,5R,1'S)-2-(1-Acetamido-3-methyl)butyl-3-(thiazol- 4-yl)- pyrrolidine-5-carboxylic Acid Dihydrochloride.

The title compound was prepared according to the method described in Example 1 K, substituting ()- (2R, 3R, 5R, 1'S)-1-t-butoxycarbonyl-2-(1-acetamido- 3-methyl) butyl-3-(thiazol-4-yl)-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3R, 5R, 1'S)-2- (l-acetamido-3-ethyl) pentyl-3-methoxymethyl-pyrrolidine-5- carboxylic acid t-butyl ester (yield : 18.5 mg, 100%).

'H NMR (DMSO-d6) : 5 0.62 (d, J = 4.2 Hz, 3H), 0.72 (d, J = 4.2 Hz, 3H), 1.05 (m, 1H), 1.12 (m, 1H), 1.30 (m, 1H), 1.72 (s, 3H), 2.14 (dt, 1H), 2.59 (dt, 1H), 3.69 (m, 1 H), 3.92 (br m, 1 H), 4.21 (m, 1 H), 4.38 (br m, 1 H), 7.46 (d, J = 1.2 Hz, 1 H), 8.02 (d, J = 5.1 Hz, 1H), 9.04 (d, J = 1.2 Hz, 1H), 9.39 (brs, 1H), 9.48 (br s, 1H).

. MS : (M+H)+= 326.

Example 32 (~)-(2R,3R,5R,1'S)-2-(1-Acetamido-3-methyl)butyl-3-(thiazol- 2-yl)-pyrrolidine-5- carboxylic Acid Dihydrochloride.

32A. (~)-(2R,3R,5R,1'S)-1-t-Butoxycarbonyl-2-(1-acetamido-3-methy l)butyl-3- carbamoyl-pvrrolidine-5-carboxylic Acid t-Butyl Ester.

()- (2R, 3R, 5R, 1'S)-1-t-Butoxycarbonyl-2- (1-acetamido-3-methyl) butyl-3- carboxyl-pyrrolidine-5-carboxylic acid t-butyl ester (0.258g, 0.584 mmol) was reacted with isobutyl chloroformate (80 mg, 0.84 mmol) and N-methylmorpholine (59 mg, 0.584 mmol) in THF (10 mL) at 0°C for 0.25 hours. Aqueous ammonium hydroxide (. 39 mL) was added and the reaction was stirred at 0°C for 0.5 hours.

The reaction was diluted with ethyl acetate. The organic layer was washed with water, and brine, dried over MgS04, filtered and concentrated in vacuo. The residue was purified by chromatography on silica gel using 100% ethyl acetate to 5 % methanol-ethyl acetate to provide the title compound, as a glass (yield : 182 mg, 70.7 %).

'H NMR (CD30D) õ 4. 70 (m, 1 H), 4.36 (q, J=3 Hz, 1 H), 4.05 (m, 1H), 2.87 (q of t, J=9 and 3 Hz, 1 H), 2.52 (m, 1 H), 2.36 (m, 1 H) 1.94 (d, 3H), 1.63 (m, 1 H), 1.41-1.53 (m, 18H), 1.3 (m, 2H), 0.9-0.18 (m, 6H) MS: (M+H) += 442 32B. (i)-(2R. 3R. 5R, 1'S)-1-t-Butoxycarbonyl-2-(1-acetamido-3-methyl) butvl-3- thiocarbamoyl-pyrrolidine-5-carboxylic Acid t-Butyl Ester.

()- (2R, 3R, 5R, 1'S)-1-t-Butoxycarbonyl-2-(1-acetamido-3-methy) butyl-3- carbamoyl-pyrrolidine-5-carboxylic acid t-butyl ester (70 mg, 0.159 mmol) was reacted with P2S10 (8.5 mg, 0.019 mmol) in 4 ml tetrahydrofuran and 1 ml of methylene chloride at room temperature. After 1.25 hrs, 9.6 mg of P2S, was added. The starting material had been consumed after 2 hrs. The mixture was diluted with ethyl acetate, washed with water and brine, dried over Mgs04, filtered and concentrated. Tlc analysis showed two spots and the mass spectrum indicated it was a mixture of mono-thio and di-thio compounds. The material was used in the next reaction without further purification.

MS: (M+H)+=458, 474 32C. ()- (2R, 3R, 5R. 1'S)-1-t-Butoxvcarbonyl-2- (1-acetamido-3-methyl) butyl-3- (thiazol-2-vl)-pyrrolidine-5-carboxylic Acid t-Butyl Ester.

()- (2R, 3R, 5R, 1'S)-1-t-Butoxycarbonyl-2-(1-acetamido-3-methy)butyl-3- thiocarbamoyl-pyrrolidine-5-carboxylic acid t-butyl ester (73 mg, o. 16 mmol) was reacted with chloroacetaldehyde (50% in water) (0.02 ml, 0.16 mmol) in 5 ml of acetone at 75°C. Magnesium sulfate (0.9 g) and additional chloroacetaldehyde was added at intervals over the next 5 hr when till complete conversion of starting material. The reaction was diluted with ethyl acetate, washed with water, and brine, dried over MgSO4. filtered and concentrated in vacuo. The residue was purified by chromatography on silica gel using 100% ethyl acetate to provide the title compound, as a glass (yield : 12.6 mg, 16.3%).

1H NMR (CDC13) 6 7.69 (m, 1 H), 7.45 (m, 1 H), 4.44 (m, 1 H), 4.28 (m, 2H), 3.52 (m,! H), 2.7 (m, 1 H), 2.5 (m, 1 H), 1.99 (s, 3H), 1.44 (s, 9H), 1.37 (s, 9H), 1.27 (m, 3 H), 0.95 (m, 6 H).

MS: (M+H)+= 482 32D. (s)-(2R. 3R. 5R. 1'S)-1-t-Butoxvcarbonyl-2-(1-acetamido-3-methyl ! butyl-3- (thiazol-2-yl)-pyrrolidine-5-carboxylic Acid Dihydrochloride The title compound was prepared according to the method described in Example 1K, substituting ()- (2R, 3R, 5R, 1'S)-1-t butoxycarbonyl-2- (1-acetamido- 3-methyl) butyl-3- (thiazol-2-yl)-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3R, 5R, 1'S)-2-(1-acetamido-3-ethyl)pentyl-3-methoxymethyl-pyrrolidi ne-5- carboxylic acid t-butyl ester (yield : 10.1 mg, 82%).

1H NMR (DMSO-d6) # 8.1 (d, J=10Hz, 1H), 7.79 (d, J=4Hz, 1H), 7.69 (d=4 Hz, 1 H), 4.49 (t, J=7.5,1 H), 4.22 (m, 1 H), 4.14 (t, J=9Hz, 1H), 4.01 (q, J=10Hz, 1 H), 2.80 (m, 1 H), 2.25 (m, 1 H), 1.78 (s, 3H), 1.47 (m, 1 H), 1.25 (m, 2H), 0.83 (d, J=6.2Hz, 3H), 0.75 (d, J=6.2Hz, 3H) MS: (M-H)-= 324, (2M-1)-= 649, (M+35)+ = 360 Example 33 (~)-(2R, 3S. 5R. 1'S)-2- (1-acetamido-3-methyl)butyl-3-(cis-2-chloro-vin-1-yl)- pvrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 33A.()- (2R. 3S, 5R, 1'S)-1-t-Butoxycarbonyl-2- (1-acetamido-3-methyl) butvl-3- (cis-2-chloro-vin-1-yl-pyrrolidine-5-carboxylic Acid t-Butyl Ester and f)- (2R, 3S. 5R. 1'S)-1-t-Butoxycarbonyl-2-(1-acetamido-3-methyl)butyl-3-(tra ns-2- chloro-vin-1-yl)-pyrrolidine-5-carboxylic Acid t-ButylEster.

The title compound was prepared according to the method described in Example 20K substituting (chloromethyl) triphenylphosphonium chloride in place of methyltriphenylphosphonium bromide. The higher Rf 0. 73 (ethyl acetate) new spot was identified to be the cis-isomer (yield : 38.4 mg, 40 %) and the lower Rf 0.57 (ethyl acetate) spot trans-isomer (yield : 42 mg, 43 %). cis-isomer'H NMR (CDC13) : 57. 44 (br, 1H), 6.13 (d, J=7.5Hz, 1H), 5.32 (dd, J=9Hz, J=7.5Hz, 1H), 4.31-4.16 (m, 2H), 3.65 (m, 1 H), 3.12 (m, 1H), 2.50 (m, 1H), 1.98 (s, 3H), 1.62 (m, 1H), 1.47 (s, 9H), 1.45 (s, 9H), 1.30-1.07 (m, 2H), 0.82 (m, 6H) MS: (M+H) += 459 trans-isomer'H NMR (CDCl3) : 8 6. 12-5.90 (m, 2H), 4.30-4.07 (m, 2H), 3.64 (m, 1H), 2.62-2.37 (m, 2H), 1.98 (s, 3H), 1.69 (m, 1H), 1.48 (s, 9H), 1.45 (s, 9H), 1.26 (m, 2H), 0.91 (m, 6H).

MS: (M+H) += 459 33B. ()- (2R, 3S. 5R. 1'S)-2-(1-acetamido-3-methyl)butyl-3-(cis-2-chloro-vin-1-yl) - pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt.

()- (2R, 3S, 5R, 1'S)-1-t-Butoxycarbonyl-2-(1-acetamido-3-methyl)butyl-3- (cis-2-chloro-vin-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (10 mg, 0.022 mmol) was reacted with trifluoroacetic acid (1.8 mL) in dichloromethane (0.4 mL) at room temperature for 7 hours. The reaction was concentrated in vacuo. The residue was dried on high vacuum to provide the title compound.

1H NMR (DMSO-d6) : 5 8.015 (d, J=7.63Hz, 1H), 6.42 (d, J=7.02Hz, 1H), 5.89 (dd, J=7.02Hz, J=8.7Hz, 1H), 4.42 (m, 1H), 4.17 (m, 1H), 3.59 (m, 1H), 3.31 (m, 1H), 2.47 (m, 1H), 1.88 (s, 3H), 1.84 (m, 1H), 1.58 (m, 1H), 1.39 (m, 1H), 1.29 (m. 2H), 0.885 (d, J=6. 71Hz, 3H), 0. 83 (d, J=6.41,3H).

MS : (M+H) += 303 Example 34 (i)-(2R 3S. 5R. 1'S)-2-(1-acetamido-3-methyl) butyl-3-(trans-2-chloro-vin-1-yl)-<BR> nyrrolidine-5-carboxviic Acid Trifluoroacetic Acid Salt 34B. (~)-(2R,3S,5R,1'S)-2-(1-acetamido-3-methyl)butyl-3-(trans-2- chloro-vinyl)- pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt.

The title compound was prepared according to the method described in Example 33B, substituting ()- (2R, 3S, 5R, 1'S)-1-t-butoxycarbonyl-2-(1-acetamido- 3-methyl) butyl-3-(trans-2-chloro-vin-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'S)-1-t-butoxycarbonyl-2- (1-acetamido-3-methyl) butyl- 3- (cis-2-chloro-vin-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester.

1H NMR (DMSO-d6) : 8 8.04 (d, J=7.93Hz, 1H), 6.355 (d, J=13. 1 Hz, 1H), 5.93 (dd, J=13. 1 Hz, J=9.32 Hz, 1H), 4.33 (m, 1H), 4.19 (m, 1H), 2.95 (m, 1H), 2.40 (m, 1H), 1.94 (m, 1H), 1.88 (s, 3H), 1.58 (m, 1H), 1.39 (m, 1H), 1.29 (m, 1H), 0.89 (d, J=6.7 Hz, 3H), 0.825 (d, J=6.7 Hz, 3H).

MS: (M+H) += 303 -Example 35 ()- 3S,5R,1'S)-2-(1-acetamido-3-methyl)butyl-3-(bis-propen-1-yl) -pyrrolidine- 5-carboxylic Acid Trifluoroacetic Acid Salt 35A. (~)-(2R,3R,5R,1'S)-1-t-Butoxycarbonyl-2-(1-acetamido-3-methy l)butyl-3-( cis-propen-1-yl !-pyrrolidine-5-carboxylic Acid t-Butyl Ester.

To a suspension of ethyl triphenylphosphonium bromide (479 mg, 1.29 mmol) in 3 mL anhydrous toluene was added potassium t-butoxide (1.0 M in THF, 0.94 mmol) dropwise at room temperature. After stirring for 2.5 (~)- (2R, 3R, 5R, 1'S)-1-t-Butoxycarbonyl-2- (1-acetamido-3-methyl) butyl-3-formyl- pyrrolidine-5-carboxylic acid t-butyl ester (90 mg, 0.211 mmol) in 5 mL toluene was added dropwise and stirred for 1 hour. The reaction was quenched with saturated aqueous ammonium chloride and diluted with ethyl acetate. The organic layer was washed with water, and brine, dried over MgS04, filtered and concentrated in vacuo. The residue was purified by chromatography on silica gel using 50% ethyl acetate/hexanes to provide the title compound, as an oil (yield : 70.6 mg, 76%).

MS: (M+H) += 439 35B. (~)-(2R,3S,5R,1'S)-2-(1-acetamido-3-methyl)butyl-3-(cis-prop en-1-yl)- pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt.

The title compound was prepared according to the method described in Example 33B, substituting ()- (2R, 3S, 5R, 1'S)-1-t-butoxycarbonyl-2-(1-acetamido- 3-methyl) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'S)-1-t-butoxycarbonyl-2- (1-acetamido-3-methyl) butyl-3- (cis-2-chloro-vinyl)-pyrrolidine-5-carboxylic acid t-butyl ester.

'H NMR (DMSO-d6) : 8 8.04 (d, J=7.5Hz, 1H), 5.51 (m, 1H), 5.26 (m, 1H), 4.32 (m, 1H), 4.18 (m, 1H), 3.45 (m, 1H), 3.18 (m, 1H), 2.39 (m, 1H), 1.88 (s, 3H), 1.73 (m, 1 H), 1.63 (dd, 3H), 1.58 (m, 1 H), 1.38 (m, 1 H), 1.28 (m, 1 H), 0.88 (d, J=6Hz, 3H), 0.81 (dd, J=6Hz, 3H).

MS: (M+H) += 283 Example 36 (~)-(2R,3S,5R,1'S)-2-(1-acetamido-3-methyl)butyl-3-(2,2-dime thyl-vin-1-yl)- pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 36A. ()- (2R, 3S, 5R, 1'S)-1-t-Butoxvcarbonyl-2- (1-acetamido-3-methyltyl-3- (2,2-dimethyl-vin-1-yl)-pyrrolidine-5-carboxylic Acid t-Butyl Ester.

The title compound was prepared according to the method described in Example 20K substituting isopropyl triphenylphosphonium iodide in place of methyltriphenylphosphonium bromide (yield : 22.6 mg, 33 %).

1H NMR (CDC13) : 8 7.77 (d, 1H), 5.06 (d, J=10Hz, 1H), 4.18 (m, 2H), 3.50 (m, 1 H), 2.69 (m, 1 H), 2.32 (m, 1 H), 1.97 (s, 3H), 1.70 (s, 3H), 1.64 (s, 3H), 1.65 (m, 1 H), 1.47 (s, 9H), 1.44 (s, 9H), 1.30-1.00 (m, 3H), 0.93 (d, J=6Hz, 3H), 0.88 (d, J=6Hz, 3H).

MS: (M+H)+= 453 36B. (~)-(2R,3S,5R,1'S)-2-(1-acetamido-3-methyl)butyl-3-(2,2-dime thyl-vin-1- yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt.

The title compound was prepared according to the method described in Example 33B, substituting ()- (2R, 3S, 5R, 1'S)-1-t-butoxycarbonyl-2- (1-acetamido- 3-methyl) butyl-3- (2, 2-dimethyl-vin-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'S)-1-t-butoxycarbonyl-2- (1-acetamido-3-methyl) butyl- 3- (cis-2-chloro-vin-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester.

1 H NMR (DMSO-d6) : # 8.01 (d, J=7.5HZ, 1H), 4.99 (d, J=10Hz, 1H), 4.30 (m, 1H), 4.14 (m, 1 H), 3.40 (m, 1H), 3.06 (m, 1H), 2.36 (m, 1H), 1.86 (s, 3H), 1.66 (s, 3H), 1.63 (s, 3H), 1.57 (m, 1H), 1.39-1.20 (m, 3H), 0.88 (d, J=6Hz, 3H) ; 0.81 (d, J=6Hz, 3H).

MS: (M+H)+= 297 Example37 (~)-(2R,3S,5R,1'S)-2-(1-acetamido-3-methyl)butyl-3-(2,2-difl uoro-vin-1-yl)- pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 37A.( (2R. 3S. 5R, 1'S)-1-t-Butoxycarbonyl-2-1-acetamido-3-methvl) butyl-3-<BR> <BR> <BR> <BR> <BR> <BR> (2. 2-difluoro-vin-1-vl)-pyrrolidine-5-carboxylic Acid t-Butvl Ester. n-Butyllithium (1.6M in hexanes, 0.61 mL, 0.97 mmol) was added to diisopropylamine (136 pL, 0.97 mmol) in 4 mL THF at-78 °C and stirred for 30 min. diethyl difluoromethylphosphonate (182 mg, 0.97 mmol) was added, the colorless solution changed slowly to yellow after stirring at-78 °C for 2 hours.

()- (2R, 3R, 5R, 1'S)-1-t-Butoxywarbonyl-2-(1-acetamido-3-methyl) butyl-3-formyl- pyrrolidine-5-carboxylic acid t-butyl ester (59 mg, 0.138 mmol) in 3 mL THF was added, stirred at-78 °C for 30 min, then warm up to room temperature. The mixture was then heated at reflux for 1.5 hour, and stirred at room temperature overnight. The reaction was quenched with saturated aqueous ammonium chloride, and diluted with ethyl acetate. The organic layer was washed with water, and brine, dried over MgS04, filtered and concentrated in vacuo. The residue was purified by chromatography on silica gel using 50% ethyl acetate/hexanes to provide the title compound, as a light yellow oil (23.4 mg, 37%).

'H NMR (CDC13) : 8 7.44 (d, 1H), 5.92 (ddd, 1H), 4.30-4.00 (m, 2H), 3.55 (m, 1 H), 2.69 (m, 1 H), 2.45 (m, 1 H), 2.00 (s, 3H), 1.47 (s, 9H), 1.43 (s, 9H), 1.45- 1.00 (m, 4H), 0.91 (m, 6H).

MS: (M+H) += 461 37B. (~)-(2R,3S,5R,1'S)-2-(1-acetamido-3-methyl)butyl-3-(2,2-difl uoro-vin-1-yl)- pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt.

The title compound was prepared according to the method described in Example 33B, substituting ()- (2R, 3S, 5R, 1'S)-1-t butoxycarbonyl-2- (1-acetamido- 3-methyl) butyl-3-(2, 2-difluoro-vin-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'S)-1-t-butoxycarbonyl-2- (1-acetamido-3-methyl) butyl-3- (cis-2-chloro-vin-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester.

'H NMR (DMSO-d6) : 5 8.04 (d, J=7.5Hz, 1 H), 4.59 (ddd, 1H), 4.23 (m, 1H), 4.14 (m, 1H), 3.48 (m, 1H), 3.39 (m, 1H), 2.91 (m, 1H), 2.43 (m, 1H), 1.85 (s, 3H), 1.58 (m, 1H), 1.40 (m, 1H), 1.31 (m, 1H), 1.22 (m, 1H), 0.89 (d, J=7.5Hz, 3H), 0.83 (d, J=7. 5Hz, 3H).

MS : (M+H) += 305 Example 38 (~)-(2R,3R,5R,1'S)-2-(1-acetamido-3-methyl)butyl-3-(pyrazol- 3-yl)-pyrrolidine-5- carboxylic Acid Trifluoroacetic Acid Salt 38A. (i)-(2R, 3R, 5R, 1'S, 1"RS)-1-t-Butoxycarbonvl-2-(1-acetamido-3- methyl)butyl-3-(1-hydroxy-2-propyn-1-yl)-pyrrolidine-5-carbo xylic Acid t-ButYl Ester.

The title compound was prepared according to the method described in Example 4A substituting 2-propynyl magnesium bromide in place of ethyl magnesium bromide and substituting ()- (2R, 3R, 5R, 1'S)-1-t-Butoxywarbonyl-2-(1- acetamido-3-methyl) butyl-3-formyl-pyrrolidine-5-carboxylic acid t-butyl ester (250 mg, 0.587 mmol) in place of ()- (2R, 3R, 5R, 1'S)-1-benzyl-2-(1-acetamido-3- ethyl) pentyl-3-formyl-pyrrolidine-5-carboxylic acid t-butyl ester the crude product was used directly in the next reaction.

MS: (M+H) +=453 38B. (~)-(2R,3R,5R,1'S)-1-t-Butoxycarbonyl-2-(1-acetamido-3-methy l)butyl-3-(1- oxo-2-propyn-1-yl-prrolidine-5-carboxylic Acid t-Butyl Ester.

()- (2R, 3R, 5R, 1'S)-1-t-Butoxycarbonyl-2-(1-acetamido-3-methyl)butyl-3-(1- hydroxy-2-propyn-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester was reacted with Jones reagent (3.0 M in acetone, 0.33 mL) in acetone (90 mL) at 0 °C to room temperature for 1 hour. The reaction was diluted with ethyl acetate. The organic layer was washed with water, and brine, dried over MgS04, filtered and concentrated in vacuo. The residue was purified by chromatography on silica gel using 50% ethyl acetate/hexanes to provide the title compound, as a white solid (yield : 143 mg, 54%).

MS: (M+H)+= 451 38C.(i)-(2R, 3R, 5R, 1'S !-1-t-Butoxvcarbonyl-2-(1-acetamido-3-methyl) butyl-3- (pyrazol-3-yl)-pyrrolidine-5-carboxylic Acid t-Butyl Ester.

(~)-(2R,3R, 5R, 1'S)-1-t-Butoxycarbonyl-2- (1-acetamido-3-methyl) butyl-3- (1- oxo-1-ethynyl) methyi-pyrrolidine-5-carboxylic acid t-butyl ester (140 mg, 0.311 mmol) was reacted with hydrazine monohydrate (0.24 mL, 4.944 mmol) in ethanol (12 mL) at room temperature for 4 hours. The reaction was concentrated in vacuo. The residue was purified by chromatography on silica gel using ethyl acetate to provide the title compound, as a white solid (yield : 131 mg, 91%).

MS : (M+H) += 465 38D. (~)-(2R,3R,5R,1'S)-2-(1-acetamido-3-methyl)butyl-3-(pyrazol- 3-yl)- pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt.

The title compound was prepared according to the method described in Example 33B, substituting ()- (2R, 3R, 5R, 1'S)-1-t-butoxycarbonyl-2-(1-acetamido- 3-methyl)butyl-3-(pyrazol-3-yl)-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3R, 5R, 1'S)-1-t-butoxycarbonyl-2-(1-acetamido-3-methyl)butyl-3-(cis -2- chloro-vinyl)-pyrrolidine-5-carboxylic acid t-butyl ester.

'H NMR (DMSO-d6) : 88. 13 (d, J=7.5Hz, 1H), 7.65 (d, J=2.2Hz, 1H), 6.20 (d, J=2.2Hz, 1H), 4.39 (m, 1H), 4.25 (m, 1H), 3.94 (m, 1H, 3.56 (q, J=7.5Hz, 1H), 2.62 (m, 1H), 2.17 (m, 1H), 1.87 (s, 3H), 1.42 (m, 1H), 1.21 (m, 1H), 1.11 (m, 1H), 0.80 (d, J=6.6Hz, 3H), 0.71 (d, J=6.6Hz, 3H).

MS: (M+H)+=309 Example 39 <BR> <BR> <BR> <BR> t)- (2R, 3R. 5R, 1'S)-2- 1-acetamido-3-methyl) buyl-3- (isoxazol-3-yl)-pyrrolidine-5- carboxylic Acid Trifluoroacetic Acid Salt and (~)-(2R,3R,5R,1'S)-2-(1-acetamido- 3-methyl) butyl-3- (isoxazol-5-yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt.

39A. (~)-(2R,3R,5R,1'S)-1-t-Butoxycarbonyl-2-(1-acetamido-3-methy l)butyl-3- (isoxazol-3-vl)-pyrrolidine-5-carboxylic Acid t-Butyl Ester and (~)-(2R,3R,5R 1'S)- 1-t-Butoxycarbonyl-2- (1-acetamido-3-methyl)butyl-3-(isoxazol-5-yl)-pyrrolidine-5- carboxylic Acid t-Butyl Ester ()- (2R, 3R, 5R, 1'S)-1-t-Butoxycarbonyl-2-(1-acetamido-3-methyl)butyl-3-(1- oxo-1-ethynyl) methyl-pyrrolidine-5-carboxylic acid t-butyl ester (31 mg, 0.07 mmol) was reacted with hydroxyamine hydrochloride (4.9 mg, 0.07 mmol) and sodium carbonate (3.7 mg, 0.035 mmol) in ethanol (3 mL) at reflux for 30 hours.

The reaction was concentrated in vacuo. The residue was purified by chromatography on silica gel using 3% methanol/dichloromethane to provide the title compound, as an oil (yield : 11.5 mg, 36%).

MS: (M+H) += 466 39B.(t)- (2R. 3R. 5R. 1'S)-21-acetamido-3-methyl) butyl-3- (isoxazol-3-yl)- pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt and (~)-(2R,3R,5R,1'S)-2- (1-acetamido-3-methyl) butvl-3-(isoxazol-5-yl)-pVrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt.

The title compound was prepared according to the method described in Example 33B, substituting ()- (2R, 3R, 5R, 1'S)-1-t-butoxycarbonyl-2-(1-acetamido- 3-methyl)butyl-3-(isoxazol-3-yl)-pyrrolidine-5-carboxylic acid t-butyl ester and ()- (2R, 3R, 5R, 1'S)-1-t-butoxycarbonyl-2-(1-acetamido-3-methyl)butyl-3-(iso xazol-5- yl)-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3R, 5R, 1'S)-1-t- butoxycarbonyl-2- (1-acetamido-3-methyl) butyl-3- (cis-2-chloro-vinyl)-pyrrolidine-5- carboxylic acid t-butyl ester.

'H NMR (DMSO-d6) : 8 8.91,8.54 (d, 1H), 8.12,8.05 (d, J=7.5Hz, 1H), 6.64,6.43 (d, 1 H), 4.48,4.51 (m, 1 H), 4.28 (m, 1 H), 3.97,3.89 (m, 1 H), 3.70,3.81 (m, 1H), 2.72 (m, 1H), 2.20,2.25 (m, 1H), 1.83,1.80 (s, 3H), 1.48 (m, 1H), 1.34- 1.10 (m, 2H), 0.83,0.84 (d, J=6Hz, 3H), 0.77,0.78 (d, J=6Hz, 3H).

MS : (M+H) +=310 Example 40 (~)-(2R,3S,5R,1'R)-2-(1-Acetamido-2-hydroxy)ethyl-3-(cis-pro pen-1-y pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 40A. (~)-(2R,3R,5R)-1-Benzyl-2-vinyl-3-(acetoxymethyl)-pyrrolidin e-5-carboxylic Acid t-Butyl Ester.

()- (2R, 3R, 5R)-1-Benzyl-2-vinyl-2-(hydroxymethyl)-pyrrolidine-5-carboxy lic acid t-butyl ester (54.2 g, 0.17 mol) and 4- (dimethylamino) pyridine (0.5 g, 4.1 mmol), in anhydrous pyridine (400 mL) was reacted with acetic anhydride (30 mL, 0.32 mol) at 0°C for 1 hour then allowed to warm to room temperature. The reaction was stirred an additional 16 hours. The pyridine was removed in vacuo at 30°C. The residue was partitioned between ethyl acetate (100 mL) and of water (400 mL). The aqueous layer was extracted with ethyl acetate (3 x 100 mL) and the combined ethyl acetate layers were washed with brine, dried with MgSO4, filtered, and concentrated. The crude product was purified by chromatography on silica gel using 10% ethyl acetate/hexanes to provide the title compound as a colorless oil (yield : 49.6 g, 81%).

'H NMR (CDC13) 6 7.28 (m, 4H), 7.21 (m, 1H), 5.68 (m, 1H), 5.21 (m, 2H), 4.16 (dd, J=6.3,10.7 Hz, 1 H), 4.10 (dd, J=7.3,10.7 Hz, 1H), 3.92 (d, J=13. 7 Hz, 1H), 3.64 (d, J=13. 7 Hz, 1H), 3.52 (m, 1H), 3.50 (m, 1H), 2.33 (m, 1H), 2.26 (m, 1H), 2.02 (s, 3H), 1.62 (m, 1H), 1.45 (s, 9H).

MS (M+H)+= 360.

40B. (~)-(2R,3R,5R,1'R)-and (~)-(2R,3R,5R,1'S)-1-Benzyl-2-(1,2- dihydroxy ethyl-3- (acetoxymethyl)-pyrrolidine-5-carboxylic Acid t-Buyl Ester.

()- (2R, 3R, 5R)-1-benzyl-2-vinyl-3-(acetoxymethyl)-pyrrolidine-5-carboxy lic acid t-butyl ester. (52.5 g, 0.15 mol) and 4-methylmorpholine N-oxide (54.7 g, 0.47 mol) in acetone (540 mL) and water (60 mL) was reacted with osmium tetroxide (200 mg, 0.8 mmol). After 24 hours, the reaction was quenched with 10% sodium thiosulfate (250 mL) concentrated in vacuo. The aqueous layer was extracted with ethyl acetate (3 x 300 mL) and the combined organic layers were washed with brine, dried over MgS04, filtered and concentrated. The residue was purified by chromatography on silica gel using a gradient elution of ethyl acetate and dichloromethane to provide the title compound as a viscous oil (yield : 41.2 g, 72%).

'H NMR (DMSO) 8 7.32 (m, 3H), 7.30 (m, 1H), 7.22 (m, 1H), 4.48 (t, J=5.4 Hz, 1 H), 4.42 (d, J=5.4 Hz, 1 H), 4.04 (m, 1 H), 4.01 (m, 1 H), 3.97 (m, 1 H), 3.80 (d, J=13. 2 Hz, 1 H), 3.78 (m, 1 H), 3.43 (m, 1 H), 3.39 (m, 1 H), 3.32 (m, 1 H), 3.07 (t, J=4.9 Hz, 1H), 2.48 (m, 1 H), 2.19 (m, 1H), 1.99 (s, 3H), 1.57 (dt, J=13. 7, 2.0 Hz, 1 H), 1.38 (s, 9H).

MS (M+H) + = 394.

40C. (~)-(2R,3R,5R,1'R) and (i)-(2R, 3R, 5R, 1'S)-2-(1, 2-Dihydroxv ! ethvl-3- (acetoxymethyl)-pvrrolidine-5-carboxvlic Acid t-Butyl Ester.

(~)-(2R, 3R, 5R, 1'R) and ()- (2R, 3R, 5R, 1'S)-1-Benzyl-2-(1, 2- dihydroxy) ethyl-3- (acetoxymethyl)-pyrrolidine-5-carboxylic acid t-butyl ester (24 g, 61 mmol) in ethanol (300 mL) was reacted with ammonium formate (38.5 g, 0.61 mol) and 10% Pd/C (2g) for 2 hours at reflux. The reaction was cooled and the catalyst removed by filtration through Celite. The filtrate was concentrated in vacuo to provide the title compound (yield : 16.7 g, 90%).

'H NMR (DMSO) 8 4.56 (m, 1H), 4.30 (m, 1H), 4.06 (dd, J=5.8,10.9 Hz, 2H), 3.79 (dd, J=8.8,10.5 Hz, 2H), 3.49 (m, 4H), 3.00 (m, 1H), 2.35 (m, 1H), 2.16 (dt, J=12.6,8.5 Hz, 1H), 2.01 (s, 3H), 1.52 (m, 1H), 1.40 (s, 9H).

MS (M+H) + = 304.

40D. (~)-(2R,3R,5R, 1'R) and (~)-(2R,3R,5R,1'S)-1-t-Butoxycarbonyl 2-(1 2- dihydroxv) ethyl-3- (acetoxymethyl)-pyrrolidine-5-carboxylic Acid t-Butyl Ester.

()- (2R, 3R, 5R, 1'R) and ()- (2R, 3R, 5R, 1'S)-2- (1, 2-Dihydroxy) ethyl-3- (acetoxymethyl)-pyrrolidine-5-carboxylic-acid t-butyl ester (33.4 g, 0.11 mol) in methanol (250 mL) and water (50 mL) was reacted with di-t-butyl dicarbonate (33.6 g, 0.15 mol) for 48 hours at room temperature. The methanol was removed in vacuo and the residue diluted with water (500 mL), and extracted with ethyl acetate (3 x 200 mL). The combined ethyl acetate layers were washed with brine, dried with MgS04, filtered and concentrated. The residue was chromatographed on silica gel using methanol/dichloromethane to provide the title compound as a white solid (yield : 32.8 g, 78%) 'H NMR (DMSO) 8 4.80 (m, 1 H), 4.45 (m, 1 H), 4.08 (m, 1 H), 3.91 (m, 2H), 3.82 (m, 1H), 3.71 (m, 1H), 3.28 (m, 2H), 2.48 (m, 1 H), 2.07 (m, 2H), 2.01 (m, 3H), 1.39 (m, 18H).

MS (M+H) + = 404.

40E (i)-(2R, 3R, 5R, 1'R) and (s)-(2R, 3R, 5R, 1'S)-1-t-Butoxvcarbonyl 2-(i- hydroxy-2-triisopropylsilyloxy)ethyl-3-(acetoxymethyl)-pyrro lidine-5-carboxylic Acid t-Butyl Ester.

()- (2R, 3R, 5R, 1'R) and ()- (2R, 3R, 5R, 1'S)-1-t-Butoxycarbonyl 2- (1, 2- dihydroxy) ethyl-3- (acetoxymethyl)-pyrrolidine-5-carboxylic acid t-butyl ester. (26.5 g, 66 mmol) in anhydrous dimethylformamide (200 mL) was reacted with imidazole (8.9 g, 0.13 mol) and triisopropylsilyl chloride (19.0 g, 99 mmol) for 4 hours at room temperature. The solvent was removed under vacuum and the residue partitioned between 300 mL of water and 150 mL of ethyl acetate. The aqueous layer was extracted with ethyl acetate (2 x 100 mL), and the combined ethyl acetate layers extracted with brine, dried with MgS04, filtered and concentrated. The residue was purified by chromatography on silica gel using 10% ethyl acetate/hexanes to provide the title compound as a colorless oil (yield : 28.9 g, 79%).

'H NMR (CDC13) 8 4. 22 (m, 1H), 4.04 (m, 3H), 3.87 (t, J=2.0 Hz, 1H), 3.74 (dd, J=4.9,9.8 Hz, 1 H), 3.58 (dd, J=7.8,10.2 Hz, 1H), 3.39 (bs, 1H), 2.61 (m, 2H). 2.03 (s, 3H), 1.75 (m, 1H), 1.46 (m, 18H), 1.07 (m, 18H).

MS (M+H) + = 560.

40F (i)- (2R. 3R. 5R)-1-t-Butoxycarbonyl-2- (1-oxo-2-triisopropylsilvloxy ethyl-3- (acetoxymethyl)-pyrrolidine-5-carboxylic Acid t-Butyl Ester.

Dimethylsulfoxide (6 mL, 85 mmol) was added slowly to a solution of oxalyl chloride (2 M) (19.3 mL, 38.6 mmol) in dry dichloromethane (70 mL) at-78°C.

After 10 minutes, a solution of ()- (2R, 3R, 5R, 1'R) and ()- (2R, 3R, 5R, 1'S)-1-t- butoxycarbonyl 2- (1-hydroxy-2-triisopropylsilyloxy) ethyl-3- (acetoxymethyl)- pyrrolidine-5-carboxylic acid t-butyl ester (14.4 g, 26 mmol) in dry dichloromethane (75 mL) was added at a rate such that the temperature did not exceed-70°C. After 1.5 hours, triethylamine (18 mL, 0.13 mol) was added and the temperature allowed to rise to 0°C. The reaction was quenched with a solution of ammonium chloride, diluted with water, and extracted with dichloromethane (3 x 100 mL). The combined dichloromethane layers were extracted with brine, dried with MgS04, filtered and concentrated. The residue was purified by chromatography on silica gel using 10% ethyl acetate/hexanes to provide the title compound as a colorless oil : (yield : 11 g, 77%).

'H NMR (CDC13) 8 4.32 (m, 6H), 2.43 (m, 2H), 2.04 (s. 3H), 1.78 (m, 1H), 1.48 (s, 9H), 1.41 (s, 9H), 1.1 (m, 21H).

MS (M+H) + = 558. 40G (i)-(2R, 3R, 5R. 1'R) and (i)-(2R, 3R, 5R. 1'S)-1-t-Butoxvcarbonvl 2-(1-amino- 2-triisopropylsilyloxy)ethyl-3-(acetoxymethyl)pyrrolidine-5- carboxylic Acid t-Butyl Ester.

()- (2R, 3R, 5R)-1-t-Butoxycarbonyl 2- (1-oxo-2-triisopropylsilyloxy) ethyl-3- (acetoxymethyl)-pyrrolidine-5-carboxylic acid t-butyl ester. (22 g, 39 mmol) in methanol (1 L) was reacted with ammonium acetate (77 g, 1.0 mol) and sodium cyanoborohydride (24.8 g, 0.39 mol) at reflux for 2 hours. The solvent was removed under in vacuo, and the residue was partitioned between water (300 mL) and dichloromethane (300 mL). The aqueous layer was extracted with dichloromethane (2 x 100 mL) and the combined organic layers were washed with brine, dried with MgS04, filtered and concentrated to provide the title compound (crude yield : 22.0g, 100%).

40H (i)-(2R, 3R, 5R, 1'R) and ()- (2R, 3R. 5R. 1'S)-1-t-Butoxycarbony1- acetamido-2-triisopropylsilyloxy)ethyl-3-(acetoxymethyl)-pyr rolidine-5-carboxylic Acid t-Butyl Ester.

()- (2R, 3R, 5R, 1'R) and ()- (2R, 3R, 5R, 1'S)-1-t-Butoxycarbonyl-2- (1-amino- <BR> <BR> 2-triisopropylsilyloxy) ethyl-3- (acetoxymethyl)-pyrrolidine-5-carboxylic acid t-butyl ester (approx 39 mmol) in dichloromethane (500 mL) was reacted with acetic anhydride (18 mL, 0.19 mol), triethylamine (27.5 mL, 0.20 mol) and dimethylaminopyridine (50 mg, 0.39 mmol) for 18 hours at room temperature.

The reaction was quenched with a solution of ammonium chloride. The aqueous layer was extracted with dichloromethane (3 x 100 mL) and the combined organic layers extracted with brine, dried with MgS04, filtered, and concentrated. The residue was chromatographed on silica gel using ethyl acetate/hexanes to provide the title compound (~)-(2R, 3R, 5R, 1'R)-1-t-butoxycarbonyl 2-(1- <BR> <BR> <BR> <BR> acetamido-2-triisopropylsilyloxy) ethyl-3- (acetoxymethyl)-pyrrolidine-5-carboxylic acid t-butyl ester (9.14 g, 39%) and ()- (2R, 3R, 5R, 1'S)-1-t-butoxycarbonyl 2-(1- <BR> <BR> <BR> acetamido-2-triisopropylsilyloxy) ethyl-3- (acetoxymethyl)-pyrrolidine-5-carboxylic acid t-butyl ester (9.75 g, 41 %) as white solids.

()- (2R, 3R, 5R, 1'R)'H NMR (CDC13) 8 7.38 (d, J=8.3 Hz, 1H), 4.34 (m, 1H), 4.20 (dd, J=2.4,10.3 Hz, 1H), 4.09 (dd, J=8.8,10.2 Hz. 1H), 4.02 (dd, J=7.3, 10.1 Hz, 1H), 3.88 (m, 1H), 3.71 (dd, J=4.4,10.3 Hz, 1H), 3.65 (dd, J=7.9,10,3 Hz, 1H), 2.74 (m, 1H), 2.60 (m, 1H), 2.04 (s, 3H), 1.98 (s, 3H), 1.69 (dt, J=14.1, 2.5 Hz, 1H), 1.46 (s, 9H), 1.42 (s, 9H), 1.07 (m, 21 H).

MS (M+H) + = 601 (i)-(2R, 3R, 5R, 1'S)'H NMR (CDC13) 8 6.82 (d, 1H), 4.10 (m, 4H), 3.81 (m, 3H), 2.55 (m, 2H), 1.98 (m, 7H), 1.46 (s, 9H), 1.42 (s, 9H), 1.07 (m, 21 H).

MS (M+H) + = 601.

401 (t)-(2R. 3R. 5R. 1'R)-1-t-Butoxycarbonyl 2-(1-acetamido-2- <BR> <BR> <BR> triisopropylsilyloxy) ethyl-3-hvdroxymethyl-pyrrolidine-5-carboxylic Acid t-Butvl Ester.

()- (2R, 3R, 5R, 1'R)-1-t-Butoxywarbonyl 2-(1-acetamido-2- <BR> <BR> <BR> triisopropylsilyloxy) ethyl-3-(acetoxymethyl)-pyrrolidine-5-carboxylic acid t-butyl ester. (8.2 g, 13.66 mmol) in methanol (200 mL) and water (50 mL) was reacted with potassium carbonate (19 g, 136 mmol) at room temperature for 2 hr. The solvent was then removed in vacuo and the residue was partitioned between water (100 mL) and dichloromethane (3 x 100 mL). The organic extracts were dried over magnesium sulfate, filtered and concentrated in vacuo to provide the title compound as a colorless oil.

40J (~)-(2R,3R,5R,1'R)-1-t-Butoxycarbonyl 2-(1-acetamido-2- triisopropvlsilyloxy) ethyl-3-formyl-pyrrolidine-5-carboxylic Acid t-Butvl Ester.

The title compound was prepared according to the method described in Example 2A substituting ()- (2R, 3R, 5R, 1'R)-1-t-butoxycarbonyl 2- (1-acetamido- <BR> <BR> (2-triisopropylsilyloxy) ethyl-3-hydroxymethyl-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3R, 5R, 1'S)-1-benzyl-2- (1-acetamido-3-ethyl) pentyl-3- hydroxymethyl-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 5.9 g, 78%).

'H NMR (CDCl3) 8 1.04-1.07 (m, 21 H), 1.42 (s, 9H), 1.43 (s, 9H), 1.99 (s, 3H), 2.42 (m, 1 H), 2.62 (m, 1 H), 3.04 (m, 1 H), 3.69 (m, 1 H), 3.82 (m, 1 H), 4.08 (m, 1 H), 4.38 (m, 1 H), 4.57 (t, 1 H), 7.33 (br d, 1 H), 9.65 (s, 1 H).

MS: (M+H) += 557.

40K )- (2R. 3S. 5R, 1'R)-1-t-Butoxycarbonyl 2- 1-acetamido-2- triisopropylsilvioxy) ethyl-3-Lcis-propen-1-yi)-pyrrolidine-5-carboxylic Acid t-Butyl Ester.

The title compound was prepared according to the method described in Example 35A substituting ()- (2R, 3R, 5R, 1'R)-1-t-butoxycarbonyl 2- (1-acetamido- 2-triisopropylsilyloxy-) ethyl-3-formyl-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3R, 5R, 1'S)-1-t-butoxycarbonyl-2- (1-acetamido-3-methyl) butyl-3- formyl-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 5.9 g, 78%).

'H NMR (CDC13) 6 1.03-1.10 (m, 21H), 1.44 (s, 9H), 1.47 (s, 9H), 1.55 (m, 1H), 1.64 (dd, 3H), 1.96 (s, 3H), 2.55 (m, 1H), 3.42 (m, 1H), 3.62-3.71 (m, 3H), 4.20 (dd, 1 H), 4.30 (m, 1 H), 5.39 (m, 1 H), 5.48 (m, 1 H), 7.73 (br d, 1 H).

MS : (M+H) += 569 40L ~)-(2R,3S,5R,1'R)-1-t-Butoxycarbonyl-2-(1-acetamido-2-hydrox y-)ethyl-3- {cis-pronen-1-yl)-nvrrolidine-5-carboxvlic Acid t-Butvl Ester.

(t)- (2R, 3S, 5R, 1'R)-1-t-Butoxycarbonyl 2- (1-acetamido-2- <BR> <BR> <BR> triisopropylsilyloxy) ethyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (4.85 g, 8.54 mmol) in THF (100 mL) was reacted with tetrabutyl ammonium fluoride (1 M in THF) (12.8 mL, 12.8 mmol) for 30 minutes at room temperature.

Water (100 mL) was added followed by extraction using dichloromethane (2 x 100 mL). This organic layers were dried over magnesium sulfate, filtered and concentrated in vacuo. The residue was purified by column chromatography on silica gel using 2/1: ethyl acetate/hexane to provide the title compound as a colorless solid (yield : 3.1 g, 89%).

'H NMR (CDC13) : d 1.44 (s, 9H), 1.47 (s, 9H), 1.56 (dd, 3H), 1.80 (m, 1H), 2.02 (s, 3H), 2.67 (m, 1 H), 3.11 (t, 3H), 3.44 (dd, 1H), 3.59 (dd, 1H), 3.74-3.84 (m, 2H), 4.15 (dd, 1 H) 5.39 (m, 1 H), 5.58 (m, 1 H), 6.42 (br d, 1 H).

MS : (M+H) += 413.

40M ()- (2R,3S,5R,1'R)-2-(1-Acetamido-2-hydroxy)ethyl-3-(cis-propen- 1-yl)- pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 33B, substituting ()- (2R, 3S, 5R, 1'R)-1-t-butoxycarbonyl-2- (1-acetamido- 2-hydroxy)ethyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'S)-1-t-butoxycarbonyl-2- (1-acetamido-3-methyl) butyl-3- (trans-2-chloro-vinyl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 18.0 mg, 100%).

'H NMR (DMSO-d6) : d 1.66 (dd, 3H), 1.71 (dt, 1H), 1.87 (s, 3H), 2.41 (dt, 1H), 3.18 (m, 1H), 3.43 (dd, 1 H), 3.61 (m, 1H), 4.13 (m, 1H), 4.35 (m, 1H), 5.25 (m, 1 H), 5.51 (m, 1H), 8.05 (d, 1 H), 9.16 (br s, 2H).

MS: (M+H) += 257.

Example 41 (~)-(2R,3S,5R,1'R,2'S)-2-(1-Acetamido-2-hydroxy)butyl-3-(cis -propen-1-yl)- pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 41A (~)-(2R,3S,5R,1'R)-1-t-Butoxycarbonyl 2-(1-acetamido-1-formyl)methyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic Acid t-Butyl Ester.

()- (2R, 3S, 5R, 1'R)-1-t-Butoxycarbonyl 2- (1-acetamido-2-hydroxy) ethyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester. (600 mg, 1.46 mmol) in dichloromethane (50 mL) was reacted with Dess-Martin Periodinane (928 mg, 2.18 mmol) for 1 hour at room temperature. The reaction was quenched with 1 M aqueous sodium thiosulfate (50 mL), stirred for 20 minutes then extracted with dichloromethane (3 x 100 mL). The organic layer was dried over magnesium sulfate, concentrated in vacuo. The residue was purified by column chromatography on silica gel using 2/1: ethyl acetate/hexane to provide the title compound (yield : 547 mg, 92%).

'H NMR (CDC13) d 9.40 (d, J= 1 Hz, 1 H), 7.88 (bd), 5.69 (m, 1 H), 5.27 (m, 1H), 4.78 (dd, J= 9.5,1. Hz, 1H), 4.21 (t, J= 8. Hz, 1H), 3.45 (m, 2H), 2.41 (m, 1 H), 2.09 (s, 3H), 1.69 (dd, J= 7.0,1. Hz, 3H), 1.55 (m, 1 H), 1.46 (s, 9H), 1.40 (s, 9H).

MS: (M+H) += 411, (M-H)-= 409.

41B (~)-(2R,3S,5R,1'R,2'R) and (~)-(2R,3S,5R,1'R,2'S)-1-t-Butoxycarbonyl-2- <BR> <BR> <BR> (1-acetamido-2-hyddroxy bu,3- (cispropen-1-vr)-pyrrolidine-5-carboxylic Acid t- Butyl Ester.

()- (2R, 3S, 5R, 1'R)-1-t-Butoxycarbonyl 2- (1-acetamido-1-formyl) methyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (780 mg, 1.90 mmol) in THF (20 mL) was added dropwise to a solution of ethylmagnesium bromide (3M in ether) (3.17 mL, 9.51 mmol) in THF (15 mL) at room temperature and reacted for 40 minutes. The reaction was quenched with water (20 mL) and saturated aqueous ammonium chloride (20 mL) followed by extraction using dichloromethane (3 x 50 mL). The organic layer was dried over magnesium sulfate, filtered and concentrated in vacuo. The residue was purified by column chromatography on silica gel using 2/1: ethyl acetate/hexane to provide the title compounds ()- (2R, 3S, 5R, 1'R, 2'R)-1-t-butoxycarbonyl 2- (1-acetamido-2- hydroxy)butyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 472 mg, 56%) and (i)-(2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl 2- (1-acetamido-2- hydroxy) butyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 82 mg, 10%) as a colorless oils.

()- (2R, 3S, 5R, 1'R, 2'R) = MS: (M+H) += 441, (M+Na) += 463, (M-H)-= 439.

()- (2R, 3S, 5R, 1'R, 2'S) = MS: (M+H) += 441, (M+Na) += 463, (M-H)-= 439.

41C ()- (2R. 3S, 5R, 1'R. 2'S)-2- 1-Acetamido-2-hvdroxy)yl-3- (cis-propen-1- yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-Butoxycarbonyl-2- (1-acetamido-2- hydroxy) butyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (300 mg, 0.68 mmol) was reacted with trifluoroacetic acid (8 mL) in dichloromethane (2 mL) at room temperature for 6 hrs. The reaction was concentrated in vacuo overnight to provide the title compound (yield : 311 mg) as a colorless solid.

'H NMR (500 MHz, DMSO-d6) ã : 7. 89 (d, J = 8. 7 Hz, 1 H), 5.48 (m, 1 H), 5.29 (m, 1H), 4.30 (m, 1H), 4.02 (m, 1H), 3.73 (m, 1H), 3.43 (m, 1H), 3.15 (m, 1H), 2.41 (m, 1H), 1.82 (s, 3H), 1.63 (m, 1H), 1.59 (dd, J= 6.8,1.9 Hz, 3H), 1.55 (m, 1 H), 1.27 (m, 1 H), 0.85 (t, J = 7.3 Hz, 3H).

MS : (M+H) += 285, (M+Na) += 307, (M-H)-= 283.

Example 42 (f)-(2R, 3S. 5R. 1'R. 2'S)-2-(1-Acetamido-2-hydroxy) butvl-3-(cis-nropen-1-vl)- pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt.

42A (~)-(2R,3S,5R,1'R)-1-t-Butoxycarbonyl-2-(1-acetamido-2-oxo)b utyl-3-(cis- propen-1-yl)-pyrrolidine-5-carboxylic Acid t-Butyl Ester.

()- (2R, 3S, 5R, 1'R, 2'R)-1-t-Butoxycarbonyl-2- (1-acetamido-2- hydroxy) butyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester. (460 mg, 1.05 mmol) was reacted with Dess-Martin Periodinane (666 mg, 1.57 mmol) in dichloromethane (30 mL) at room temperature for 17 hours. The reaction was quenched with 1 M aqueous sodium thiosulfate (50 mL and stirred for 20 minutes.

The reaction was was extracted with dichloromethane (3 x 100 mL). The organic layer was dried over magnesium sulfate, filtered, and concentrated in vacuo. The residue was purified by column chromatography on silica gel using 2: 1: ethyl acetate/hexane to provide the title compound as a colorless semi-solid (yield : 440 mg, 96%).

MS: (M+H) += 439, (M+Na) += 461, (M-H)-= 437.

42B (i)-(2R. 3S. 5R. 1'R, 2'R) and (~)-(2R,3S,5R,1'R,2'S)-1-t-Butoxycarbonyl-2-<BR> <BR> <BR> (1-acetamido-2-hvdroxY) bil-3- (cis-propen-1-vl)-pyrrolidine-5-carboxylic Acid t-<BR> <BR> Butyl Ester.

()- (2R, 3S, 5R, 1'R)-1-t-Butoxycarbonyl-2- (1-acetamido-2-oxo) butyl-3- (cis- propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (435 mg, 0.99 mmol) in methanol (30 mL) was reacted with sodium borohydride (188 mg, 4.97 mmol) at room temperature for 0.5 hours. The solvent was removed in vacuo and water (30 mL) was added. The aqueous layer was extracted with dichloromethane (3 x 50 mL). This organic layer was dried over magnesium sulfate, filtered and concentrated in vacuo. The residue was purified by column chromatography on silica gel using 2: 1 ethyl acetate/hexane to provide the title compounds ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2- (1-acetamido-2-hydroxy) butyl-3- (cis- propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester. (yield : 305 mg, 70%) and compounds ()- (2R, 3S, 5R, 1'R, 2'R)-1-t-butoxycarbonyl-2- (1-acetamido-2- hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 17 mg, 4%).

42C (~)-(2R,3S,5R,1'R,2'S)-2-(1-Acetamido-2-hydroxy)butyl-3-(cis -propen-1- yl-pyrrolidine-5-carbocylic Acid Trifluoroacetic Acid Salt ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-Butoxycarbonyl-2-(1-acetamido-2- hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (300 mg, 0.68 mmol) was reacted with trifluoroacetic acid (8 mL) in dichloromethane (2 mL) at room temperature for 6 hrs. The reaction was concentrated in vacuo overnight and triturated with acetonitrile (2 x 5 mL) to provide the title compound (yield: 311 mg) as a colorless solid.

'H NMR (500 MHz, DMSO-d6) 8 : 7.89 (d, J = 8.7 Hz, 1 H), 5.48 (m, 1 H), 5.29 (m, 1H), 4.30 (m, 1H), 4.02 (m, 1H), 3.73 (m, 1H), 3.43 (m, 1H), 3.15 (m, 1 H), 2.41 (m, 1 H), 1.82 (s, 3H), 1.63 (m, 1 H), 1.59 (dd, J = 6.8,1.9 Hz, 3H), 1.55 (m, 1 H), 1.27 (m, 1 H), 0.85 (t, J = 7.3 Hz, 3H).

MS: (M+H) += 285, (M+Na) += 307, (M-H)-= 283.

Example 43 (t)-(2R, 3S, 5R, 1'R. 2'R)-2- (1-Acetamido-2-hydroxy butvl-3- (cis-propen-1-yl-<BR> <BR> nyrrolidine-5-carboxvlic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41C, substituting ()- (2R, 3S, 5R, 1'R, 2'R)-1-t-butoxycarbonyl-2-(1- acetamido-2-hydroxy) butyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2- (1-acetamido-2- hydroxy)butyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : . 0065 g, 100%).

'H NMR (DMSO-d6) 8 7.90 (d, J=8.8Hz, 1H), 5.47 (m, 1H), 5.29 (t, J=9.8 Hz, 1H), 4.29 (t, J=8.8Hz, 1H), 4.02 (q, J=6.8Hz, 1H), 3.71 (bt, J=8Hz, 1H), 3.43 (m, 1H), 3.15 (quint., J=8.8Hz, 1H), 2.41 (dt, J=12.7,7.8Hz, 1H), 1.82 (s, 3H), 1.64 (m, 1H), 1.58 (dd, J=6.8,1.5Hz, 3H), 1.53 (m, 1H), 0.85 (t, J=7.3Hz, 3H).

MS: (M+H)'= 285, (M+Na) + = 307, (M-H)-=283, (M+CF3COOH)- = 397, (2M-1)-= 563 Example 44 (~)-(2R,3S,5R,1'R,2'S)-2-(1-Acetamido-2-hydroxy-3-cyano)prop yl-3-(cis-propen- 1-yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 44A(~)-(2R,3S,5R,1'R,2'R) and (~)-(2R,3S,5R,1'R,2'S)-1-t-Butoxycarbonyl-2- (1-acetamido-2-hydroxy-3-cyano) propyl-3- (cis-propen-1-yl)-pyrrolidine-5- carboxylic Acid t-Butyl Ester.

()- (2R, 3S, 5R, 1'R)-1-t-Butoxycarbonyl 2-(1-acetamido-1-formyl)methyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (150 mg, 0.37 mmol) in THF (10 mL) was added dropwise to a solution of the lithium enolate of acetonitrile (1.83 mmol, 5 equivalents) in THF (15 mL) at-78 °C and reacted for 15 minutes. The reaction was quenched with saturated aqueous ammonium chloride (10 mL) and water (10mL) followed by extraction using dichloromethane (2 X 50 mL). The organic layer was dried over magnesium sulfate, filtered and concentrated in vacuo. The residue was purified by column chromatography on silica gel using 2/1: ethyl acetate/hexane to provide the title compounds (~)- (2R, 3S, 5R, 1'R,2'R)-1-t-butoxycarbonyl 2-(1-acetamido-2-hydroxy-3-cyano)propyl- 3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 95mg, 58%) and ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl 2-(1-acetamido-2-hydroxy-3- cyano) propyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 30 mg, 18%) as a colorless oils.

()- (2R, 3S, 5R, 1'R, 2'R) = MS: (M+H)+=452, (M-H)-=450 ()- (2R, 3S, 5R, 1'R, 2'S) ='H NMR (CDC13) 8 8.14 (d, J=8.9Hz, 1H), 5.51 (m, 1 H), 5.38 (m, 1 H), 4.25 (m, 1H), 4.19 (m, 1H), 3.94 (m, 1H), 3.74 (m, 1H), 3.22 (m, 1H), 2.54 (m, 1H), 2.47 (m, 2H), 2.04 (s, 3H), 1.69 (m, 1H), 1.65 (dd, J=6.5, 1.8Hz, 3H), 1.47 (s, 9H), 1.45 (s, 9H) MS: (M+H)+=452, (M-H)-=450 44 ()- (2R. 3S, 5R, 1'R. 2'S)-21-Acetamido-2-hydroxy-3-cyano) propyl-3- (cis- propen-1-yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41 C, substituting ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- acetamido-2-hydroxy-3-cyano)propyl-3-(cis-propen-1-yl)-pyrro lidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- acetamido-2-hydroxy) butyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 4.5 mg, 95% %).

1H NMR (DMSO-d6) 5 7.98 (d, J=10. 0 Hz, 1H), 5.49 (m, 1H), 5.27 (m, 1H), 4.30 (m, 1H), 4.15 (m, 1H), 3.75 (m, 1H), 3.18 (m, 1H), 2.72-2.58 (m, 2H), 2.41 (m, 1H), 1.85 (s, 3H), 1.65 (m, 1H), 1.61 (dd, J=6.70,1.80 Hz, 3H) MS: (M+H)+=296, (M-H)-=294 Example 45 (~)-(2R,3S,5R,1'R,2'R)-2-(1-Acetamido-2-hydroxy-3-cyano)prop yl-3-(cis-propen- 1-yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41 C, substituting ()- (2R, 3S, 5R, 1'R, 2'R)-1-t-butoxycarbonyl-2-(1- <BR> <BR> <BR> acetamido-2-hydroxy-3-cyano) propyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- acetamido-2-hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 8 mg, 95%).

'H NMR (DMSO-d6) 8 7.75 (d, J=9.0 Hz, 1H), 5.47 (m, 1H), 5.25 (m, 1H), 4.46 (m, 1H), 4.20 (m, 1H), 4.13 (m, 1H), 3.56 (m, 1H), 3.15 (m, 1H), 2.55 (m, 2H), 2.42 (m, 1H), 1.82 (s, 3H), 1.72 (m, 1H), 1.55 (dd, J=6.71,1.83,3H) MS: (M+H) + =296, (M+23) + =318, (M-H)-=294 Example 46 -f2R.3S.5R.1'R.2'R)-2-1-Acetamido-2-hvdroxv-3-ethoxvcarbonv) )DroDv!-3-c/s- propen-1-vl)-nyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 46A ()- (2R. 3S. 5R. 1'R. 2'R) and ()- (2R, 3S. 5R, 1'R, 2'S)-1-t-Butoxycarbonyl-2- (1-acetamido-2-hydroxy-3-ethoxycarbonyl)propyl-3-(cis-propen -1-yl)-pyrrolidine- 5-carboxylic Acid t-Butyl Ester.

()- (2R, 3S, 5R, 1'R)-1-f-Butoxycarbonyl-2- (1-acetamido-1-formyl) methyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (900 mg, 2.187 mmol) in THF (40 mL) was added dropwise to a solution of the lithium enolate of ethyl acetate (8.75 mmol, 4 equivalents) in THF (40 mL) at-78 °C and reacted for 15 minutes. The reaction was quenched with saturated aqueous ammonium chloride followed by extraction using dichloromethane (3 X). The organic layer was dried over magnesium sulfate, filtered and concentrated in vacuo. The residue was purified by column chromatography on silica gel using 1: 1 ethyl acetate/hexane to provide the title compounds ()- (2R, 3S, 5R, 1'R, 2'R)-1-t- butoxycarbonyl-2- (1-acetamido-2-hydroxy-3-ethoxycarbonyl) propyl-3- (cis-propen- 1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 690 mg, 63%) (~)- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1-acetamido-2-hydroxy-3- ethoxycarbonyl) propyl-3-. (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 246 mg, 22.5%).

()- (2R, 3S, 5R, 1'R, 2'R)'H NMR (CDCI3) : # 5.99 (d, 1H), 5.60 (m, 1H), 5.36 (m, 1H), 4.81 (m, 1H), 4.15 (m, 4H), 3.74 (m, 1H), 3.07 (m, 1H), 2.68 (m, 1H), 2.48 (m 1H), 2.33 (m, 1H), 2.03 (s, 3H), 1.54 (dd, 3H), 1.47 (s, 9H), 1.46 (s, 9H), 1.24 (t, J=7.5Hz, 3H).

MS: (M+H) +=499 ()- (2R, 3S, 5R, 1'R, 2'S)'H NMR (CDCl3) : # 7. 93 (d, 1H), 5.44 (m, 2H), 4.19 (m, 4H), 4.03 (m, 1H), 3.72 (m, 1H), 3.37 (m, 1H), 2.63 (m. 1H), 2.48 (m, 2H), 2.01 (s, 3H), 1.65 (dd, 3H), 1.48 (s, 9H), 1.46 (s, 9H), 1.26 (t, J=7.5Hz, 3H).

MS: (M+H) +=499 46B (~)-(2R,3S,5R,1'R,2'R)-2-(1-Acetamido-2-hydroxy-3- ethoxycarbonyl) propyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41C, substituting ()- (2R, 3S, 5R, 1'R, 2'R)-1-t-butoxycarbonyl-2-(1- <BR> <BR> <BR> <BR> acetamido-2-hydroxy-3-ethoxycarbonyl) propyl-3- (cis-propen-1-yl)-pyrrolidine-5- carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl- 2- (1-acetamido-2-hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t- butyl ester.

'H NMR (DMSO-d6) : # 7.74 (d, J=9Hz, 1H), 5.48 (m, 1H), 5.25 (m, 1H), 4.43 (m, 1H), 4.24 (m, 1H), 4.14 (m, 1H), 4.06 (q, J=7.5Hz, 2H), 3.54 (m, 1H), 3.16 (m, 1H), 2.41 (m, 1H), 2.36 (m, 2H), 1.82 (s, 3H), 1.77 (m, 1H), 1.56 (dd, 3H), 1.18 (t, J=7.5Hz, 3H).

MS: (M+H) += 343 Example 47 (~)-(2R,3S, 5R. 1'R. 2'S)-2-(1-Acetamido-2-hydroxy-3-ethoxycarbonyl)propyl-3-(cis - propen-1-yl)-pvrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound is prepared according to the method described in Example 41C, substituting ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- acetamido-2-hydroxy-3-ethoxycarbonyl) propyl-3-(cis-propen-1-yl)-pyrrolidine-5- carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl- <BR> <BR> <BR> <BR> 2- (1-acetamido-2-hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t- butyl ester.

1H NMR (DMSO-d6) : 8 7.93 (d, J=9Hz, 1H), 5.48 (m, 1H), 5.30 (m, 1H), 4.19 (m, 1H), 4.09 (m, 1H), 4.06 (q, J=7.5Hz, 2H), 3.94 (m, 1H), 3.73 (m, 1H), 3.18 (m, 1H), 2.54 (dd, 1H), 2.40 (m, 1H), 2.27 (m, 1H), 1.82 (s, 3H), 1.65 (m, 1H), 1.60 (dd, 3H), 1.19 (t, J=7.5Hz, 3H).

MS: (M+H) +=343 Example 48 (~)-(2R,$S,5R,1'R,2'S)-2-(1-Acetamido-2-hydroxy)propyl-3-(ci s-propen-1-yl)- pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41 C, substituting ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxywarbonyl-2-(1- acetamido-2-hydroxy)propyl-3-(cis-propen-1-yl)-pyrrolidine-5 -carboxylic acid t- butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2- (1-acetamido- 2-hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 0.0030 g, 100%).

1H NMR (DMSO-d6) d 8.97 (bs, 1H), 7.88 (d, J=8.5 Hz, 1H), 5.45 (m, 1H), 5.28 (t, J = 9. 1Hz, 1H), 4.30 (t, J=8.6Hz, 1H0, 3.94 (q, J=7.3Hz, 1H), 3.71 (t, J=8. OHz, 1H), 3.62 (m, 1H), 3.15 (quint., J=9. OHz, 1H), 2.40 (dt, J=12. 8, 7.6Hz, 1H), 1.83 (s, 3H), 1.65 (m, 1H), 1.59 (dd, J=7.0,1.5Hz, 3H), 1.08 (d, J=5.5Hz, 3H).

MS: (M+H)+=271, (M+Na) + = 293, (M-H)-= 269.

Example 49 (~)-(2R,3S,5R, 1'R. 2'R)-2-(1-Acetamido-2-hydroxy)propyl-3-(cis-propen-1-yl)- pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41 C, substituting ()- (2R, 3S, 5R, 1'R, 2'R)-1-t-butoxycarbonyl-2-(1- acetamido-2-hydroxy)ethyl-3-)-cis-propen-1-yl)-pyrrolidine-5 -caboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1-acetamido-2- hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 0.0143 g, 100%).

'H NMR (DMSO-d6)# 7.70 (d, J=9.1 Hz, 1H), 5.49 (m, 1H), 5.25 (t, = 9. 1Hz, 1H), 4.43 (t, J=8.6Hz, 1H), 4.03 (m, 1H), 3.92 (m, 1H), 3.55 (t, J=8.5Hz, 1H), 3.17 (quint., J=8.5Hz, 1H), 2.42 (dt, J=12. 8,7.3Hz, 1H), 1.85 (s, 3H), 1.72 (dt, J=12.8,10. 0Hz, 1H), 1.57 (dd, J=6.7,1.8Hz, 3H), 1.04 (d, J=6. 1Hz, 3H).

MS: (M+H) +=271, (M+Na)+=293, (M-H)-= 269 Example 50 <BR> <BR> (i)-(2R. 3S. 5R. 1'R, 2'S)-2-(1-Acetamido-2-hydroxy-2-vinvl) ethvl-3-(cis-propen-1-<BR> <BR> <BR> vl)-Dyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt.

50A(~)-(2R,3S,5R,1'R,2'S) and (~)-(2R,3S,5R,1'R,2'R)-1-t-Butoxycarbonyl-2- <BR> <BR> <BR> (1-acetamido-2-hydroxv-2-vinyl ethvl-3- (cis-propen-1-yi)-pyrrolidine-5-carboxylic Acidt-Butyl Ester.

The title compounds were prepared according to the method described in Example 41 B, substituting vinyl magnesium bromide for ethyl magnesium bromide to provide ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2- (1-acetamido-2- hydroxy-2-vinyl) ethyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 6.5 mg, 18%) and ()- (2R, 3S, 5R, 1'R, 2'R)-1-t-butoxycarbonyl-2-(1- acetamido-2-hydroxy) ethyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 22 mg, 59%).

()- (2R, 3S, 5R, 1'R, 2'S) MS: (M+H) +=439, (M-H)-= 437 ()- (2R, 3S, 5R, 1'R, 2'R) MS: (M+H) +=439, (M-H)-= 437 50B (~)-(2R,3S,5R,1'R,2'S)-2-(1-Acetamido-2-hydroxy-2-vinyl)ethy l-3-(cis- propen-1-vl)-pyrrolidine-5-carboxvlic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41 C, substituting ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- acetamido-2-hydroxy-2-vinyl) ethyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- acetamido-2-hydroxy) butyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 5 mg, 96%).

1H NMR (DMSO-d6) #7. 85 (d, J=9.1Hz, 1H), 5.76 (m, 1H), 5.47 (m, 1H), 5.25 (m, 2H), 5.14 (m, 1H), 4.29 (m, 1H), 4.05 (m, 1H), 3.96 (m, 1H), 3.71 (m, 1H), 3.18 (m, 1H), 2.41 (m, 1H), 1.78 (s, 3H), 1.64 (m, 1H), 1.59 (dd, J=6.71,1. 21Hz, 3H) MS : (M+H) + =283, (M+23) + =305, (M-H)-=281 Example 51 ()- (2R. 3S. 5R, 1'R. 2'R)-2- (1-Acetamido-2-hydroxv-2-vinyl) ethvl-3- (cis-propen-1- yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41C, substituting ()- (2R, 3S, 5R, 1'R, 2'R)-1-t-butoxycarbonyl-2-(1- acetamido-2-hydroxy-2-vinyl) ethyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- acetamido-2-hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-bytyl ester (yield : 6 mg, 95%).

1H NMR (DMSO-d6) # 7.84 (d, J=9.7Hz, 1H), 5.78 (m, 1H), 5.48 (m, 1H), 5.23 (m, 34.43 (m, 1H), 4.26 (m, 1H), 4.20 (m, 1H), 3.55 (m, 1H), 3.18 (m, 1H), 2.43 (m, 1H), 1.81 (s, 3H), 1.73 (m, 1H), 1.57 (dd, J=6.72,1.83 HZ, 3H) MS : (M+H) + =283, (M+23)-=305, (M-H)-=281, (2M-H)'=563 Example 52 (~)-(2R,3S, 5R. 1'R. 2'S)-2- (1-Acetamido-2-hydroxy-2-vinyl) propy(cis-propen-1- yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt.

52A (i)-(2R. 3S. 5R. 1'R. 2'S) and (~)-2R,3S,5R,1'R,2'R)-1-t-Butoxycarbonyl-2- <BR> <BR> <BR> (1-acetamido-2-hydroxy-3-vinyl) propyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic<BR> <BR> <BR> <BR> Acid t-Butyl Ester.

The title compounds were prepared according to the method described in Example 41 B, substituting allyl magnesium bromide for ethyl magnesium bromide to provide ()- (2R, 3S, 5R, 1'R, 2'S)-t-butoxycarbonyl-2-(1-acetamido-2-hydroxy- 2-vinyl)propyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 2.0 mg, 5%) and ()- (2R, 3S, 5R, 1'R, 2'R)-1-t-butoxycarbonyl-2- (1-acetamido-2- hydroxy) propyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 9.0 mg, 22%).

()- (2R, 3S, 5R, 1'R, 2'S) MS : (M+H) +=453 ; (M-H)-= 451.

()- (2R, 3S, 5R, 1'R, 2'R)'H NMR (DMSO-d6) 8 7.70 (d, J=9.3Hz, 1H), 5.80 (m, 1 H), 5.51 (m, 1 H), 5.30 (m, 1 H), 5.00 (m, 2H), 4.58 (br d, 1 H), 3.93 (m, 2H), 3.50 (m, 1H), 3.22 (br t, 1H), 2.02 (m, 3H), 1.88 (s, 3H), 1.56 (m, 4H), 1.41 (s, 9H), 1.36 (s, 9H) MS : (M-H)-= 451; (M+H) + = 452, (M+Na) + = 475.

52B (~)-(2R,3S,5R,1'R,2'S)-2-(1-Acetamido-2-hydroxy-3-vinyl)prop yl-3-(cis- propen-1-yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41C, substituting ()- (2R, 3S, 5R, 1'R, 2'S)-1-t butoxycarbonyl-2- (1- acetamido-2-hydroxy-2-vinyl) propyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t butoxycarbonyl-2- (1- acetamido-2-hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 2 mg, 100%).

'H NMR (DMSO-d6) 8 7.85 (d, J=9.3Hz, 1H), 5.81 (m, 1H), 5.42 (m, 1H), 5.28 (t, J=7.3Hz, 1 H), 5.01 (br d, 2H), 3.99 (m, 2H), 3.57 (m, 2H), 3.08 (m, 1 H), 2.33 (m, 1 H), 2.26 (m, 1 H), 2.07 (m, 1 H), 1.81 (s, 3H), 1.57 (dd, J=1.4,5.4Hz, 4H) MS: (M-H)-= 295 ; (M+H)+ 297, (M+Na)+= 319.

Example 53 (t)- (2R, 3S. 5R. 1'R. 2'R)-2- (1-Acetamido-2-hvdroxy-3-vinyl) propyl-3- (cis-propen-1-<BR> <BR> <BR> <BR> <BR> yl)-nvrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41C, substituting ()- (2R, 3S, 5R, 1'R, 2'R)-1-tbutoxycarbonyl-2-(1- acetamido-2-hydroxy-3-vinyl)propyl-3-(cis-propen-1-yl)-pyrro lidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2(1- acetamido-2-hydroxy)butyl-3-(cis-propen-1-yl)-pyrrolidine-5- carboxylic acid t-butyl ester (yield: 6 mg, 100%).

'H NMR (DMSO-d6) 5 7.68 (d, J=9.2Hz, 1H), 5.78 (m, 1H), 5.48 (m, 1H), 5.24 (t, J=7.8Hz, 1H), 5.04 (m, 2H), 4.38 (t, J=7.0,1H), 4.09 (t, J=7.0, 1H), 3.81 (t, J=4.7, 1H), 3.53 (t, J=8.5, 1H), 3.16 (m, 1H), 2.40 (m, 1H), 2.11 (m, 2H), 1.83 (s, 3H), 1.70 (m, 1H), 1.55 (dd, J=5.4,1.4Hz, 3H) MS: (M-H)-= 295 ; (M+H) + = 297, (M+Na) + = 319.

Example 54 (~)-(2R,3S,5R, 1'R. 2'S)-2-(1-Acetamido-2-hydroxy)pentyl-3-(cispropen-1-yl)- pyrrolidine-5-carboxvlic Acid Trifluoroacetic Acid Salt.

54A (i)-(2R. 3S, 5R. 1'R, 2'S) and ~)-2R,3S,5R, 1'R. 2'R)-1-t-Butoxycarbonvl-2- <BR> <BR> <BR> <BR> (1-acetamido-2-hydroxv) penyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxlic Acid t- Butyl Ester.

The title compounds were prepared according to the method described in Example 41 B, substituting propyl magnesium bromide for ethyl magnesium bromide to provide ()- (2R, 3S, 5R, 1'R, 2'S)-t-t-butoxycarbonyl-2-(1-acetamido-2- hydroxy) pentyl-3-(cix-propen-1-yl)-pyrrolidne-5-carboxylic acid t-butyl ester (yield : 1 mg, 1%) and ()- (2R, 3S, 5R, 1'R, 2'R)-1-t-butoxycarbonyl-2-(1-acetamido- 2-hydroxy)pentyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxyli c acid t-butyl ester (yield : 32 mg, 39%).

()- (2R, 3S, 5R, 1'R, 2'S)'HNMR (CDC13) 8 7.51 (d, J=8.2Hz, 1H), 5.46 (m, 2H), 4.17 (dd, J=3.1,6.8Hz, 1 H), 4.05 (m, 1H) 3.81 (t, J=3.4Hz, 1H), 3.54 (m, 1H), 3.21 (m, 1H), 2.60 (m, 1H), 2.02 (s, 3H), 1.70 (dt, J=3.0,7.4Hz, 1H), 1.61 (d, J=5.4Hz, 3H), 1.54 (m, 1H), 1.47 (s, 9H), 1.44 (s, 9H), 1.32 (m, 4H), 0.90 (t, J=7.1 Hz, 3H) MS: (M+H) + = 455, (M+Na) + = 477; (M-H)-= 453.

()- (2R, 3S, 5R, 1'R, 2'R)'H NMR (CDC13) 8 5.98 (d, J=9.5Hz, 1H), 5.60 (t, J=9.8Hz, 1H), 5.36 (m, 1H), 4.16 (m, 1H), 3.75 (d, J=10. 1Hz, 1H), 3.64 (m, 1H), 3.51 (m, 1H), 3.09 (brt, 1H), 2.68 (m, 1H), 2.02 (s, 3H), 1.81 (d, J=13. 9Hz, 1 H), 1.57 (m, 4H), 1.54 (dd, J=1. 7,5.1 Hz, 3H), 1.46 (s, 9H), 1.45 (s, 9H), 0.88 (t, J=6.8Hz, 3H) MS: (M-H)-= 453; (M+H) + = 455.

54B (~)-(2R,3S,5R,1'R,2'S)-2-(1-Acetamido-2-hydrox)pentyl-3-(cis -propen-1- vl)-pvrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41C, substituting (~)-(2R,3S,5R,1'R,2'S)-1-t-butoxycarbonyl-2-(1- acetamido-2-hydroxy) pentyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t- butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2- (1-acetamido- 2-hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 1 mg, 100%).

'H NMR (DMSO-d6) 8 7.83 (d, J=9.2Hz, 1 H), 5.43 (m, 1 H), 5.23 (m, 1H), 3.98 (m, 1 H), 3.56 (br t, 1 H), 3.46 (m, 1 H), 3.08 (m, 2H), 2.32 (m, 1 H), 1.80 (s, 3H), 1.57 (dd, J=1.4,5.4Hz, 4H), 1.43 (m, 2H), 1.23 (m, 2H), 0.85 (br t, 3H) MS: (M+H) + = 299, (M+Na) + = 321.

Example 55 (~)-(2R,3S,5R,1'R,2'S)-2-(1-Acetamido-2-hydroxy)pentyl-3-(ci s-propen1-yl)- pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41C, substituting ()- (2R, 3S, 5R, 1'R, 2'R)-1-t-butoxywarbonyl-2-(1- acetamido-2-hydroxy) pentyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t- butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1-acetamido- 2-hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 0. 0190 g, 100%).

1H NMR (DMSO-d6) 8 7.64 (d, J=9.3Hz, 1H), 5.48 (m, 1H), 5.24 (m, 1H), 4.38 (t, J=8.8Hz, 1H), 4.06 (m, 1H), 3.75 (m, 1H), 3.53 (t, J=8.5Hz, 1H), 3.16 (quint., J=8.5Hz, 1H), 2.41 (dt, J=12.8,7.3Hz, 1H), 1.82 (s, 3H), 1.70 (dt, 12.8,9.9Hz, 1H), 1.55 (dd, J=7.0,1.6Hz, 3H), 1.35 (m, 2H), 1.26 (m 2H), 0.86 (t, J=6.7Hz, 3H).

MS: (M+H) += 299, (M+Na)+=321, (M-H)-= 297.

Example 56 (i)-(2R. 3S. 5R. 1'R. 2'S)-2- (1-Acetamido-2-hydroxy-3-methyl) butyl-3- (cis-propen-1- yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt.

56A (i)-(2R, 3S, 5R, 1'R, 2'S) and (~)-(2R,3S,5R,1'R,2'S)-1-t-Butoxycarbonyl-2 (1-acetamido-2-hydroxy-3-methyl)butyl-3-(cis-propen-1-yl)-py rrolidine-5- carboxylic Acid t-Butyl Ester.

The title compounds were prepared according to the method described in Example 41 B, substituting isopropyl magnesium bromide for ethyl magnesium bromide to provide ()- (2R, 3S, 5R, 1'R, 2'S)-1-t butoxycarbonyl-2- (1-acetamido-2- hyd roxy-3-methyl) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 0.0092 g, 10%) and ()- (2R, 3S, 5R, 1'R, 2'R)-1-t-butoxycarbonyl-2-(1- acetamido-2-hyeroxy-3-methyl)butyl-3-(cis-propen-1-yl)-pyrro lidine-5-carboxylic acid t-butyl ester (yield : 0.0385 g, 40%).

()- (2R, 3S, 5R, 1'R, 2'S) MS: (M+H) += 455, (M+Na) += 477, (2M+Na) +=931, (M-H)-=453.

(f)- (2R, 3S, 5R, 1'R, 2'R) MS: (M+H) += 455, (M+Na) += 477, (2M+Na) += 931, (M-H)-= 453.

56B (~)-(2R,3S,5R,1'R,2'S)-2-(1-Acetamido-2-hydroxy-3-methyl)but yl-3-(cis- propen-1-yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41C, substituting ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- <BR> <BR> <BR> <BR> acetamido-2-hydroxy-3-methyl) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- acetamido-2-hydroxy) butyl-3-(cix-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 0.010 g, 100%).

1H NMR (DMSO-d6) d 7.63 (d, J=9.2Hz, 1H), 5.48 (m, 1H), 5.23 (m, 1H), 4.44 (m, 1H), 4.24 (m, 1H), 3.57 (t, J=8.7Hz, 1H), 3.33 (dd, J=8.5,2.5Hz, 1H), 3.21 (quint., J=9. 1Hz, 1H), 2.43 (dt, J=12. 8, 7.6Hz, 1H), 1.81 (s, 3H), 1.73 (dt, J=12.8,10.4Hz, 1H), 1.56 (dd, J=6.7,1.9Hz, 3H), 1.55 (m, 1H), 0. 94 (d, J=6.7Hz, 3H), 0.78 (d, J=6.7Hz, 3H).

MS: (M+H)+=299, (M+Na)+=321, (M-H)-= 297, (M+CF3COOH)-=411, (2M-H)-= 595.

Example 57 (~)-(2R,3S,5R,1'R,2'S)-2-(1-Acetamido-2-hydroxy-3-methyl)but yl-3-(cis-propen-1- yl)-yrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41C, substituting ()- (2R, 3S, 5R, 1'R, 2'R)-1-t-butoxycarbonyl-2-(1- acetamido-2-hydroxy-3-methyl)butyl-3-(cis-propen-1-yl)-pyrro lidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butyoxycarbonyl-2-(1- acetamido-2-hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 0.0433 g, 100%).

'H NMR (DMSO-d6) d 7.88 (d, J=9.2Hz, 1 H), 5.46 (m, 1H), 5.29 (m, 1H), 4.26 (t, J=8.5Hz, 1H), 4.11 (m, 1H), 3.67 (m, 1H), 3.39 (dd, J=9.8,1.8Hz, 1H), 3.15 (quint., J=9. 1Hz, 1H), 2.42 (dt, J=12.8,7.9Hz, 1H), 1.81 (s, 3H), 1.73 (m, 1H), 1.62 (m, 1H), 1.57 (dd, J=7.0,1.6Hz, 3H), 0.88 (d, J=6.7Hz, 3H), 0.75 (d, J=6.7Hz, 3H).

MS: (M+H)+= 299, (M+Na)+=321, (M-H2O)+= 281, (M-H)-= 297, (M+CF3COOH)-= 411, (2M-H)-= 595.

Example 58 (t)-i (2R. 3S. 5R, 1'R. 2'S)-2- (1-Acetamido-2-hydroxy) hexyl-3- (cis-propen-1-yl pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt.

58A (~)-(2R,3S,5R,1'R, 2'S) and (i)-(2R, 3S, 5R, 1'R, 2'R)-1-t-Butoxycarbonyl-2- <BR> <BR> <BR> (1-acetamido-2-hydroxy hexyl-3- (cis-propen-1-vl)-pyrrolidine-5-carboxylic Acid t- Butyl Ester.

The title compounds were prepared according to the method described in Example 41 B, substituting butyl magnesium bromide for ethyl magnesium bromide to provide ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butyoxycarbonyl-2-(1-acetamido-2- hydroxy) hexyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 2.0 mg, 8%) and ()- (2R, 3S, 5R, 1'R, 2'R)-1-t-butoxycarbonyl-2- (1-acetamido-2- hydroxy) hexyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 6.0 mg, 24%). 58B (~)-(2R,3S,5R,1'R,2'S)-2-(1-Acetamido-2-hydroxy)hexyl-3-(cis -propen-1- yl)-pyrrolidine-5-carboxylic-Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41C, substituting ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butyoxycarbonyl-2-(1- acetamido-2-hydroxy)hexyl-3-(cis-propen-1-yl)-pyrrolidine-5- carboxylic acid t- butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2- (1-acetamido- 2-hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 2.0 mg, 100%).

'H NMR (DMSO-d6) 8 8.34 (d, J=9.3Hz, 1H), 5.24 (m, 1H), 5.12 (m, 1H), 3.90 (m, 1H), 3.78 (m, 1H), 3.23 (m, 1H), 2.90 (m, 1H), 2.14 (m, 1H), 1.80 (m, 1 H), 1.75 (s, 3H), 1.52 (m, 3H), 1.45 (m, 1 H), 1.08 (br s, 6H), 0.83 (br t, 3H).

MS: (M-H)-= 311 ; (M+H) + = 313.

Example 59 ()-2R, 3S, 5R, 1'R, 2'R)-2- (1-Acetamido-2-hvdroxy) hexyl-3- (cis-propen-1-y)- pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41C, substituting ()- (2R, 3S, 5R, 1'R, 2'R)-1-t-butoxycarbonyl-2-(1- acetamido-2-hydroxy) hexyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t- butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'R)-1-t-butoxycarbonyl-2-(1-acetamido- 2-hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 6. 0 mg, 100%).

'H NMR (DMSO-d6) # 7.60 (d, J=9. 3 Hz, 1H), 5.46(m,1H), 5.24 (t, J=9.2 Hz, 1H), 4.21 (t, J=8.3 Hz, 1H), 4.02 (t, J=7.9Hz, 1H), 3.74 (m, 1H), 3.47 (t, J=8.8, 1H), 3.12 (m, 1H), 2.37 (m, 1H), 1.81 (s, 3H), 1.64 (m, 1H), 1.55 (dd, J=1.5,5.4 Hz, 3H), 1.29 (m, 6H), 0.86 (t, J=6.9,3H) MS: (M-H)-= 311 ; (M+H) + = 313, (M+Na) + = 335.

Example 60 (t)- (2R, 3S, 5R. 1'R, 2'S)-2-1-Acetamido-2-hydroxv-4-methyl) penyl-3- (cis-propen-<BR> <BR> 1-vl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt.

60A (~)-(2R,3S,5R,1'R,2'R)-2-(1-acetamido-2-hydroxy-4- methv!)pentv)-3-(c/s-propen-1-v))-pvrro)idine-5-carboxv)ic Acid t-Butyl Ester.

The title compounds were prepared according to the method described in Example 41 B, substituting isobutyl magnesium bromide for ethyl magnesium bromide to provide ()- (2R, 3S, 5R, 1'R, 2'R)-1-t-butoxycarbonyl-2-(1-acetamido-2 hydroxy-4-methyl)pentyl-3-(cis-propen-1-yl)-pyrrolidine-5-ca rboxylic acid t-butyl ester (yield : 31 mg, 51%).

()- (2R, 3S, 5R, 1'R, 2'R)'H NMR (CDC13) 8 5.98 (d, J=9.5Hz, 1H), 5.61 (t, J=8.2Hz, 1 H), 5.35 (m, 1H), 4.51 (dd, J=1.3,3.1Hz, 1H), 4.15 (m, 1H), 3.74 (d, J=10. 5Hz, 1H), 3.61 (m, 2H), 3.09 (t, J=7.5Hz, 1H), 2.71 (m, 1H), 2.02 (s, 3H), 1.81 (d, J=13. 9Hz, 1H), 1.58 (br s, 1H), 1.54 (dd, J=1.7,5. 1Hz, 3H), 1.47 (s, 9H), 1.45 (s, 9H), 1.42 (m, 1 H), 0.87 (dd, J=2.4,6.7Hz, 6H) MS: (M-H)-= 467; (M+H) + = 469, (M+Na) + = 491.

60B ()- (2R. 3S. 5R, 1'R)-1-t-Butoxvcarbonyl-2- (1-acetamido-4-methvl-2-<BR> <BR> <BR> <BR> <BR> oxo) pentyl-3- (cis-propen-1-yl)-pvrrolidine-5-carboxylic Acid t-Butyl Ester.

()- (2R, 3S, 5R, 1'R, 2'R)-1-t-Butoxycarbonyl-2- (1-acetamido-2-hydroxy-4- methyl) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (8.0 mg, 0.02 mmol) was reacted with Dess-Martin Periodinane (10 mg, 0.03 mmol) in dichloromethane (0.1 mL) at room temperature for 1 hour. The reaction was quenched with 1 M aqueous sodium thiosulfate 1 mL and stirred for 20 minutes.

The reaction was extracted with dichloromethane (3 x 1 mL). The organic layer was dried over magnesium sulfate, filtered, and concentrated in vacuo. The residue was purified by column chromatography on silica gel using 1: 1: ethyl acetate/hexane to provide the title compound as a colorless semi-solid (yield : 4.8 mg, 61%).

60C (~)-(2R,3S,5R,1'R,2'S)-1-t-Butoxycarbonyl-2-(1-acetamido-2-h ydroxy-4- methyl)pentyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic Acid t-ButylEster.

()- (2R, 3S, 5R, 1'R)-1-t-Butoxycarbonyl-2-(1-acetamido-4-methyl-2- oxo) pentyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (4.8 mg, 0.01 mmol) in methanol (0.1 mL) was reacted with sodium borohydride (2.0 mg, 0.05 mmol) at room temperature for 0.5 hours. The solvent was removed in vacuo and water (1 mL) was added. The aqueous layer was extracted with dichloromethane (3 x 1 mL). This organic layer was dried over magnesium sulfate, filtered and concentrated in vacuo. The residue was purified by column chromatography on silica gel using 1: 1 ethyl acetate/hexane to provide the title compound ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2- (1-acetamido-2-hydroxy- 4-methyl) pentyl-3-(cis-propen-1-yl)-pyrroldine-5-carboxylic acid t-butyl ester (yield : 2. 4 mg, 51%).

60B (i)-(2R. 3S. 5R. 1'R, 2'S)-2-(1-Acetamido-2-hydroxy4-methyl) pentyl-3-(cis- propen-1-yl)-pvrrolidine-5-carboxvlic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41C, substituting ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- <BR> <BR> <BR> acetamido-2-hydroxy4-methyl) pentyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t butoxycarbonyl-2- (1- acetamido-2-hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 4.4 mg, 100%).

'H NMR (D20) 8 5.45 (m, 1 H), 5.15 (t, J=11.0Hz, 1H), 3.88 (m, 1 H), 3.62 (t, J=8. 0Hz, 1 H), 3.43 (br t, 1 H), 2.98 (m, 1 H), 2.36 (m, 1 H), 1.81 (s, 3H), 1.60 (m, 1H), 1.51 (m, 1H), 1.45 (dd, J=1.3,5.4Hz, 3H), 1.17 (m, 3H), 0.74 (dd, J=6.7, 14Hz, 6H) MS: (M-H)-= 311 ; (M+H) + = 313, (M+Na) + = 335.

Example 61 ()- (2R. 3S, 5R. 1'R, 2'R)-2- 1-Acetamido-2-hydroxy-4-methyl) pentyl-3- (cis-propen-<BR> <BR> <BR> <BR> <BR> <BR> 1-yl'i-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41C, substituting ()- (2R, 3S, 5R, 1'R, 2'R)-1-t-butoxycarbonyl-2-(1- acetamido-2-hyeroxy-4-methyl-pentyl-3-(cis-propen-1-yl)-pyrr olidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- acetamido-2-hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 1.7 mg, 85%).

'H NMR (DMSO-d6) 8 7.61 (d, J=9.8Hz, 1H), 5.45 (m, 1H), 5.24 (t, J=7.4Hz, 1 H), 4.29 (br t, 1 H), 4.0 (br t, 1 H), 3.83 (m, 1 H), 3.49 (t, J=8.8Hz, 1 H), 3.13 (m, 1 H), 2.39 (m, 1H), 1.82 (s, 3H), 1.68 (m, 2H), 1.55 (dd, J=1.4,5.4Hz, 3H), 1.31 (m, 1 H), 1.04 (m, 1 H), 0.86 (dd, J=6.4,8.3Hz, 6H) MS : (M-H)-= 311 ; (M+H) + = 313, (M+Na) + = 335.

Example 62 (t)- (2R. 3S. 5R. 1'R. 2'S)-2- (1-Acetamido-2-hydroxv) pent-3-ynvl)-3- (cis-propen-1- yl)yrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt. 62A ()- (2R, 3S. 5R. 1'R. 2'S) and (~)-(2R,3S,5R,1'R,2'R)-1-t-Butoxycarbonyl-2-(1- Acetamido-2-hydroxy)pent-3-ynyl)-3-(cis-prrolidine-5-carboxy lic Acid t-Butyl Ester.

The title compounds were prepared according to the method described in Example 41 B, substituting propyn-1-yl zinc for ethyl magnesium bromide to provide ()- (2R, 3S, 5R, 1'R, 2'S)-1-t butoxycarbonyl-2- (1-acetamido-2- hydroxy)pent-3-ynyl-3-(cis-propen-1-yl)-pyrrolidine-5-carbox ylic acid t-butyl ester (yield : 0.0073 g, 16%) and ()- (2R, 3S, 5R, 1'R, 2'R)-1-t-butoxycarbonyl-2-(1- <BR> <BR> <BR> <BR> acetamido-2-hydroxy) pent-3-ynyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 0.0349 g, 77%).

()- (2R, 3S, 5R, 1'R, 2'S) MS: (M+H) +=451, (M+Na) +=473, (2M+Na) +=923,. (M-H)-=449.

(~)-(2R,3S, 5R, 1'R, 2'R) MS: (M+H) += 451, (M+Na) +=473, (2M+Na) +=923, (M-H)=449.

62B (~)-(2R, 3S. 5R. 1'R. 2'S)-2-(1-Acetamido-2-hydroxy)pent-3ynyl)-3-(cis- propen-1-yl)-pyrrolidine-5-carboxvlic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41C, substituting ()- (2R, 3S, 5R, 1'R, 2'R)-1-t-butoxycarbonyl-2-(1- <BR> <BR> <BR> <BR> acetamido-2-hydroxy) pent-3-ynyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2- (1- acetamido-2-hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 0.0052 g, 100%).

1H NMR (DMSO-d6) d 7.97 (d, J=8.3 Hz, 1H), 5.48 (m, 1H), 5.25 (m, 1H), 4.35-4.20 (m, 3H), 3.67 (m, 1H), 3.18 (quint., 8.8Hz, 1H), 2.41 (dt, J=12.7, 7.8Hz, 1H), 1.84 (s, 3H), 1.81 (d, J=1.9Hz, 3H), 1.63 (m, 1H), 1.59 (dd, J=6.9,2. 0Hz, 3H).

MS : (M=H)+= 295, (M+Na)+= 317, (M-H)-= 293, (M+CF3COO-)-=407, (2M- H)-=587.

Example63 ()- (2R. 3S. 5R. 1'R, 2'R)-2-f1-Acetamido-2-hvdroxv)Dent-3-vnv))-3-fc/s-DroDen-1- vl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41C, substituting ()- (2R, 3S, 5R, 1'R, 2'R)-1-t-butoxycarbonyl-2-(1- <BR> <BR> <BR> acetamido-2-hydroxy) pent-3-ynyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- acetamido-2-hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 0.0540 g, 100%).

1H NMR (DMSO-d6) d 7.90 (d, J=8.8 Hz, 1H), 5.50 (m, 1H), 5.25 (m, 1H), 4.40-4.35 (m, 2H), 4.28 (m, 1H), 3.71 (t, J=8.0 Hz, 1H), 3.18 (quint., 8.3Hz, 1H), 2.42 (dt, J=13.2,7.4Hz, 1H), 1.87 (s, 3H), 1.82 (d, J=1.9Hz, 3H), 1.71 (dt, J=12.7,10. 0Hz, 1H), 1.57 (dd, J=6.9,1.5Hz, 3H).

MS: (M+H)+=295, (M+Na) + = 317, (M-H)-= 293, (M+CF3COO-)-=407.

Example 64 (f)-(2R, 3S. 5R. 1'R. 2'R)-2-(1-Acetamido-2-hvdroxy-2-hentafluoronronvl) ethvl-3- (cis-propen-1-yl)-pyyrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 64A (t2R. 3S, 5R, 1'R, 2'R)-1-t-Butoxycarbonyl-2-1-acetamido-2-hydroxv-2-<BR> <BR> <BR> <BR> <BR> <BR> heptafluoropropvl) ethy(cis-propen-1-yl-pvrrolidine-5-carboxylic Acid t-Butyl Ester.

()- (2R, 3S, 5R, 1'R)-1-t-Butoxycarbonyl 2-(1-acetamido-1-formyl) methyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (41 mg, 0.10 mmol) and heptafluoropropyl iodide (0.144 mL, 1.0 mmol, 10 equivalents) in THF (2 mL) were reacted with 1M phenylmagnesium bromide (0.90 mL, 0.90 mmol, 9 equivalents) at-78 °C for 5 minutes. The reaction mixture was allowed to warm to room temperature over 1 h. The reaction was quenched with saturated aqueous ammonium chloride (10 mL) and water (10 mL) followed by extraction using ethyl acetate (3 X 25 mL). The organic layer was dried over magnesium sulfate, filtered and concentrated in vacuo. The residue was purified by column chromatography on silica gel using 1/2 : ethyl acetate/hexane to provide the title compound (~)- (2R, 3S, 5R, 1'R, 2'R)-1-t-butoxycarbonyl-2-(1-acetamido-2-hydroxy- <BR> <BR> <BR> <BR> 2-heptafluoropropyl) ethyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 12.6 mg, 22%).

(t)- (2R, 3S, 5R, 1'R, 2'R) MS: (M+H) +=581, (M+Na) +=603, (2M+Na) +=1183, (M-H)-=579.

64B (~)-(2R,3S,5R,1'R,2'S)-2-(1-Acetamido-2- heptafluoropropyl) ethvl-3- (cis-propen-1-vl)-prrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41C, substituting ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- acetamido-2-hydroxy-2-heptafluoropropyl) ethyl-3-(cis-propen-1-yl)-pyrrolidine-5- carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'R)-1-t-butoxycarbonyl-2-(1-acetamido- 2- (1-acetamido-2-hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t- butyl ester (yield : 0.003 g, 100%).

1H NMR (DMSO-d6) d 7. 84 (d, J=9.3 Hz, 1H), 5.45 (m, 1H), 5.26 (m, 1H), 4.71 (t, J=9.7 Hz, 1 H), 4.63 (d, J=22.0 Hz, 1 H), 4.51 (m, 1 H), 3.59 (t, J=9.3 Hz, 1H), 3.19 (quint., 8.3Hz, 1H), 2.43 (dt, J=12. 7, 7.3Hz, 1H), 1.76 (s, 3H), 1.74 (m, 1H), 1.53 (dd, J=6.8,1.4Hz, 3H).

MS: (M+H)+= 425, (M+Na) + = 447, (M-H)-= 423, (2M-1)'= 847.

Example 65 (~)-(2R, 3S. 5R. 1'R. 2'S)-2-(1-Acetamido-2, 4-dihydroxy) butyl-3-(cis-propen-1-vl)- pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 65A (i)-(2R. 3S. 5R. 1'R. 2'S)-1-t-Butoxycarbonyl-2-(1-acetamido-2, 4- dihydroxv) butvl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxvlic Acid t-Butyl Ester.

()- (2R, 3S, 5R, 1'R, 2'S)-1-t-Butoxycarbonyl-2- (1-acetamido-2-hydroxy-3- ethoxycarbonyl) ethyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (35 mg, 0.07 mmol) was reacted with lithium borohydride (8 mg, 0.35 mmol) in THF (5 mL) at 25°C and reacted for 3 hours. The reaction was quenched with saturated aqueous ammonium chloride (2 mL) and water (2 mL) followed by extraction using dichloromethane (2 X 10 mL). The organic layer was dried over magnesium sulfate, filtered and concentrated in vacuo. The residue was purified by column chromatography on silica gel using 5% methanol in dichloromethane to provide the title compound ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- acetamido-2,4-dihydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t- butyl ester (yield : 14 mg, 44%).

()- (2R, 3S, 5R, 1'R, 2'S) =MS: (M+H) +=457, (M-H)-=455 65B (~)-(2R,3S,5R,1'R,2'S)-2-(1-Acetamido-2,4-hydroxy)butyl-3-(c is-propen- 1-v!)-pvrro)idine-5-carboxv)ic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41C, substituting ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- acetamido-2,4-dihydroxy) butyl 3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t- butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2- (1-acetamido- 2-hydroxy)butyl-3-9cis-propen-1-yl)-pyrrolidine-5-carboxylc acid t-butyl ester.

1H NMR (DMSO-d6) 8 7.93 (d, J=9. OHz, 1H), 5.56 (m, 1H), 5.31 (m, 1 H), 4.43 (m, 1H), 4.14 (m, 1H), 3.69 (m, 1H), 3.63 (m, 1H), 3.23 (m, 2H), 3.07 (m, 1 H), 2.43 (m, 1 H), 2.06 (s, 3H), 1.83 (m, 2H), 1.79 (m, 1 H), 1.62 (dd, J=6.71,1.22 Hz, 3H) MS: (M+H)+=301, (M-H)-=299.

Example 66 (~)-(2R,3S,5R,1'R,2'S)-2-(1-Acetamido-2,4-hydroxy)butyl-3-(c is-propen-1-yl)- eyrrolidine-5-carboxvlic Acid Trifluoroacetic Acid Salt 66A A (2R, 3S. 5R. 1'R. 2'R)-1-t-Butoxycarbonyl-2- 1-acetamido-2. 4- dihydroxy) butyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic Acid t-Butyl Ester.

The title compound was prepared according to the method described in Example 65A substituting ()- (2R, 3S, 5R, 1'R, 2'R)-1-t-butoxywarbonyl-2-(1- acetamido-2-hydroxy-3-ethoxycarbonyl)ethyl-3-9cis-propen-1-y l)-pyrrolidine-5- carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'R)-1-t-butoxycarbonyl- 2- (1-acetamido-2-hyd roxy-3-ethoxycarbonyl) ethyl-3- (cis-propen-1-yl)-pyrrolidine- 5-carboxylic acid t-butyl ester. (yield : 11 mg, 70%).

'H NMR (CDCl3) # 5.58 (m, 1H), 5.38 (m, 1H), 4.16 (m, 1H), 4. 05(m, 1H), 3.97 (m, 1 H), 3.78 (m, 2H), 3.20 (m, 1 H), 2.66 (m, 1 H) 2.54 (m, 1 H), 2.04 (s, 3H), 1.80 (m, 1H), 1.55 (m, 2H), 1.47 (s, 9H), 1.44 (s, 9H) MS: (M+H) +=457, (M-H)-=455 66B (~)-(2R,3S,5R,1'R,2'S)-2-(1-Acetamido-2,4-hydroxy)butyl-3-(c is-propen- 1-yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41C, substituting ()- (2R, 3S, 5R, 1'R, 2'R)-1-t-butoxycarbonyl-2-(1- acetamido-2,4-dihydroxy) butyl 3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t- butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1-acetamido- 2-hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 8 mg, 96%).

'H NMR (DMSO-d6) 8 7.91 (d, J=9. 1Hz, 1H), 5.50 (m, 1H), 5.25 (m, 1H), 4.43 (m, 1 H), 4.30 (m, 1 H), 4.22 (m, 1 H), 3.94 (m, 1 H), 3.86 (m, 1 H), 3.62 (m, 1H), 3.18 (m, 1H), 2.43 (m, 1H), 1.85 (s, 3H), 1.75 (m, 1H), 1.65 (m, 2H), 1.58 (dd, J=6.70,1.81 Hz, 3H).

MS: (M+H)+=301, (M-H)-=299.

Example67<BR> <BR> <BR> (i)-(2R. 3S. 5R. 1'R. 2'S)-2-(1-Acetamido-2-hydroxy-2-(phenylacetvlen-1-vl)) ethyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxvlic Acid Trifluoroacetic Acid Salt 67A (~)-(2R,3S,5R, 1'R. 2'R) and (t) (2R, 3S. 5R, 1'R. 2'S)-1-t-Butoxycarbonyl-2- <BR> <BR> <BR> (1-acetamido-2-hvdroxy-2- (phenvlace len-1-yl)) ethyl-3- (cis-propen-1-yl)-<BR> <BR> <BR> pyrrolidine-5-carboxylic Acid t-Butyl Ester.

The title compounds were prepared according to the method described in Example 41 B, substituting lithium phenylacetylide for ethyl magnesium bromide to provide ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2- (1-acetamido-2-hydroxy- 2-(phenylacetylen-1-yl))ethyl-3-(cis-propen-1-yl)-pyrrolidie n-5-carboxylic acid t- butyl ester (yield : 0.0010 g, 4%) and ()- (2R, 3S, 5R, 1'R, 2'R)-1-t-butoxycarbonyl- 2- (1-acetamido-2-hydroxy-2- (phenylacetylen-1-yl)) ethyl-3- (cis-propen-1-yl)- pyrrolidine-5-carboxylic acid t-butyl ester (yield : 0.0050 g, 21%).

(~)-(2R,3S,5R,1'R, 2'S) MS: (M+H) +=513, (M+Na) +=535, (2M+Na) +=1047, (M-H)-=511.

()- (2R, 3S, 5R, 1'R, 2'R) MS: (M+H) +=513, (M+Na) +=535, (2M+Na) +=1047, (M-H)-=511.

67B (i)-(2R. 3S. 5R. 1'R. 2'S)-2- (1-Acetamido-2-hydroxy-2- (phenylaceylen-1- <BR> <BR> <BR> <BR> yl)) ethvl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound is prepared according to the method described in Example 41 C, substituting ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- acetamido-2-hydroxy-2-(phenylacetylen-1-yl)ethyl-3-(cis-prop en-1-yl)-pyrrolidine- 5-carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t- <BR> <BR> <BR> <BR> butoxycarbonyl-2- (1-acetamido-2-hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5- carboxylic acid t-butyl ester.

Example 68 <BR> <BR> <BR> <BR> <BR> <BR> ()- (2R. 3S, 5R, 1'R. 2'R)-2- 1-Acetamido-2-hydroxy-2- (phenylaceylen-1-yl)) ethyl- 3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 68A (W2R.3S.5R.1'R.2'R)-2-n-Acetamido-2-hvdroxv-2-(Dhenviacetv!e n-1- yl)) ethyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41C, substituting ()- (2R, 3S, 5R, 1'R, 2'R)-1-t-butoxycarbonyl-2-(1- acetamido-2-hydroxy-2- (phenylacetylen-1-yl)) ethyl-3- (cis-propen-1-yl)-pyrrolidine- 5-carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t- <BR> <BR> <BR> butoxycarbonyl-2- (1-acetamido-2-hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5- carboxylic acid t-butyl ester (yield : 0.0034 g, 100%).

'H NMR (DMSO-d6) 8 9.2 (bs, 1H), 8.04 (d, J=9.2 Hz, 1H), 7.45-7.35 (m, 5H), 5.50 (m, 1 H), 5.29 (m, 1 H), 4.64 (d, J=4.9,1 H), 4.5-4.4 (m, 2H), 3.81 (m, 1H), 3.22 (quint., J=8.5Hz, 1H), 2.45 (dt, J=12.8,7.3Hz, 1H), 1.89 (s, 3H), 1.74 (dt, J=12.7,10. 0Hz, 1H), 1.58 (dd, J=7.3,1.8Hz, 3H).

MS: (M+H) += 357, (M+Na) + = 379, (M-H)-= 355.

Example 69 <BR> <BR> <BR> <BR> <BR> <BR> ()- (2R. 3S. 5R, 1'R. 2'S)-2- (1-Acetamido-2-hydroxy-3-ethvl) penyl-3- (cis-propen-1- vl)-nvrrolidine-5-carboxviic Acid Trifluoroacetic Acid Salt 69A ()- 2R, 3S, 5R, 2'R)-1-t-butoxycarbonyl-2-(1-acetamido-2-oxo-3-<BR> <BR> ethy!)pentvt-3-(c/s-proDen-1-v))-pyrro!idine-5-carboxy!icAci d-Buty) Ester.

The title compound was prepared according to the method described in Example 42A, substituting ()- (2R, 3S, 5R, 1'R, 2', R)-1-t-butoxycarbonyl-2-(1- <BR> <BR> acetamido-2-hyd roxy-3-ethyl) pentyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester in place of (2R, 3S, 5R, 1'R, 2'R)-1-t-butoxycarbonyl-2-(1- <BR> <BR> acetamido-2-hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield: 8 mg, 81 %).

MS: (M+H) +=481, (M-H)'=479 69B (_)-(2R. 3S. 5R, 1'R, 2'S)-1-t-Butoxvcarbonyl-2-(1-Acetamido-2-hvdroxy-3- ethyl)pentyl-3-(cis-propen-1-yl)-pyrrolidine-5-caboxylic Acid t-Butyl Ester.

The title compound was prepared according to the method described in Example 42B, substituting ()- (2R, 3S, 5R, 1'R)-1-t-butoxycarbonyl-2- (1-acetamido- 2-oxo-3-ethyl) pentyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester in place of (2R, 3S, 5R, 1'R)-1-t-butoxycarbonyl 2- (1-acetamido-2-oxo) butyl-3- (cis- propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 5 mg, 63%).

MS: (M+H) +=483, (M-H)-=481 69C()-(2R.3S.5R.1'R.2'S)-2-n-Acetamido-2-hvdroxv-3-ethv!)Den tvt-3-fc/'s- propen-1-yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41C, substituting ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- <BR> <BR> acetamido-2-hyd roxy-3-ethyl) pentyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester in place of (~)-(2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- <BR> <BR> acetamido-2-hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 4 mg, 95%).

'H NMR (DMSO-d6) 8 7.67 (d, J=8.9Hz, 1H), 5.48 (m, 1H), 5.23 (m, 1H), 4.42 (m, 1H), 4.21 (m, 1H), 3.58 (m, 2H), 3.22 (m, 1 H), 2.43 (m, 1H), 1.82 (s, 3H), 1.74 (m, 1H), 1.58 (dd, J=6.71,1.23 Hz, 3H), 1.52 (m, 1H), 1.38 (m, 1H), 1.29 (m, 2Hz), 1.13 (m, 1H), 0.80 (m, 6H) MS: (M+H) +=327, (M-H)-=325 Example 70 (~)-(2R,3S,5R,1'R,2'S)-2-(1-Acetamido-2-hydroxy-3-ethyl)pent yl-3-(cis-propen-1- yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 70A (~)-(2R,3S,5R,1'R,2'S)-1-t-butoxycarbonyl-2-(1-Acetamido-2-h ydroxy-3- ethyl)pentyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic Acid t-Butyl Ester.

The title compound was prepared according to the method described in Example 41 B, substituting 3-pentyl magnesium bromide in place of ethyl magnesium bromide (yield: 13mg, 45%).

MS: (M+H) +=483, (M-H)-=481 70B (~)-(2R,3S,5R,1'R,2'S)-2-(1-Acetamido-2-hydroxy-3-ethyl)pent yl-3-(cis- propen-1-yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41 C, substituting (~)-(2R, 3S, 5R, 1'R, 2'R)-1-t-butoxycarbonyl-2-(1- <BR> <BR> <BR> acetamido-2-hydroxy-3-ethyl) pentyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- acetamido-2-hydroxy) butyl-3-(cis-propent-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 3 mg, 96%).

1H NMR (DMSO-d6) 8 7.85 (d, J=9.2 Hz, 1H), 5.47 (m, 1H), 5.30 (m, 1H), 4.28 (m, 1H), 4.19 (m, 1H), 3.67 (m, 1H), 3.58 (m, 1H), 3.17 (m, 1H), 2.43 (m, 1H), 1.81 (s, 3H), 1.63 (m, 1H), 1.58 (dd, J=6.71,1.82 Hz, 3H), 1.40 (m, 2H), 1.28 (m, 1H), 1.10 (m, 1H), 1.05 (m, 1H), 0.83 (m, 6H) MS: (M+H)+=327, (M-H)-=325 Example 71 (~)-(2R,3S,5R,1'R,2'S)-2-(1-Acetamido-2-hydroxy-2-phenyl)eth yl-3-(cis-propen-1- yl)-pvrrolidine-5-carboxvlic Acid Trifluoroacetic Acid Salt 71A (~)-(2R, 3S. 5R. 1'R. 2'R)-1-t-butoxycarbonyl-2-(1-Acetamido-2-hydroxy-2- phenvl) ethyl-3- (cis-propen-1-yl)-pvrrolidine-5-carboxylic Acid t-Butvl Ester.

The title compound was prepared according to the method described in Example 41 B, substituting phenyl magnesium bromide in place of ethyl magnesium bromide (yield : 36 mg, 60%).

MS: (M+H) += 489, (M+Na) += 511, (M-H)-= 487.

71B (~)-(2R,3S,5R,1'R,2'S)-2-(1-Acetamido-2-hydroxy-2-phenyl)eth yl-3-(cis- propen-1-vl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41 C, substituting ()- (2R, 3S, 5R, 1'R, 2'R)-1-t-butoxycarbonyl-2- (1- <BR> <BR> acetamido-2-hydroxy-2-phenyl) ethyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid. t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- acetamido-2-hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 5.5 mg, 100%).

1H NMR (DMSO-d6) d 7.79 (d, J= 9.2Hz, 1H), 7.36 (m, 2H), 7.31 (m, 2H), 7.22 (m, 1H), 5.49 (m, 1H), 5.22 (m, 1H), 4.94 (d, J= 3. OHz, 1 H), 4.52 (m, 1 H), 4.35 (m, 1 H), 3.62 (t, J= 8.5Hz, 1 H), 3.22 (m, 1 H), 2.46 (m, 1 H), 1.77 (m, 1 H), 1.65 (s, 3H), 1.57 (dd, J= 6.7,0.8Hz, 3H).

MS: (M+H) += 333, (M+Na) + = 355, (M-H)-= 331.

Example 72 <BR> <BR> <BR> <BR> <BR> <BR> <BR> ()- (2R, 3S, 5R, 1'R. 2'S)-2- (1-Acetamido-2-hydroxy-2-phenyl) ethyl-3- (cis-propen-1- yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 72A (i)-(2R, 3S. 5R. 1'R)-1-t-Butoxycarbonyl-2-(1-Acetamido-2-oxo-2- phenv yl)ethyl-3-(cis-propen-1-yl)-pyrrolidien-5-carboxylic Acid t-Butyl Ester.

The title compound was prepared according to the method described in Example 42A, substituting ()- (2R, 3S, 5R, 1'R, 2'R)-1-t-butoxycarbonyl-2-(1- acetamido-2-hydroxy-2-phenyl) ethyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester in place of (2R, 3S, 5R, 1'R, 2'R)-1-t-butoxycarbonyl-2-(1- acetamido-2-hydroxy)butyl-3-(cis-propen-1-yl)-pyrrolidine-5- carboxylic acid t-butyl ester (yield : 24 mg, 84%).

MS: (M+H)+=487, (M+Na)+= 509, (M-H)-= 485.

72B (~)-(2R, 3S. 5R. 1'R. 2'S)-1-t-Butoxycarbonyl-2-(1-Acetamido-2-hydroxy-2- phenyl) ethyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic Acid t-Butyl Ester.

The title compound was prepared according to the method described in Example 42B, substituting ()- (2R, 3S, 5R, 1'R)-1-t-butoxywarbonyl-2-(1-acetamido- 2-oxo-2-phenyl)ethyl-3-(cis-propen-1-yl)-pyyrolidine-5-carbo xylic acid t-butyl ester in place of (2R, 3S, 5R, 1'R)-1-t-butoxycarbonyl 2- (1-acetamido-2-oxo) butyl-3- (cis- propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 7.9 mg, 52%).

MS: (M+H)+= 489, (M+Na) += 520, (M-H)-= 487.

72C (i)-(2R, 3S. 5R, 1'R, 2'S)-2-(1-Acetamido-2-hydroxy-2-nhenyl) ethyl-3-(cis- propen-1-yl)-pyrrolidine-5-carboxrlic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41C, substituting ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- acetamido-2-hydroxy-2-phenyl)ethyl-3-cis-propen-1-yl)-pyrrol idine-5-carboxylic acid t-butyl ester in place of (~)-(2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- Acetamido-2-hydroxy)butyl-3-(cis-propen-1-yl)-pyrrolidine-5- carboxylic acid t-butyl ester (yield : 7.5 mg, 100%).

'H NMR (DMSO-d6) d 7.83 (d, J= 9.2Hz, 1H), 7.36 (m, 2H), 7.32 (m, 2H), 7.25 (m, 1 H), 5.47 (m, 1 H), 5.33 (m, 1 H), 4.54 (d, J= 9.8Hz, 1 H), 4.36 (m, 1 H), 4.23 (m, 1 H), 3.78 (m 1 H), 3.20 (m, 1 H), 2.43 (m, 1 H), 1.63 (m, 1 H), 1.56 (dd, J= 6.7,1.2Hz, 3H), 1.53 (s, 3H).

MS: (M+H) += 333, (M+Na) += 355, (M-H)-= 331.

Example 73 (i)-(2R. 3S. 5R, 1'R. 2'R)-21-Acetamido-2-hydroxv-2- (thiophen-2-yl)) et(cis- propen-1-yl-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 73A (s)-(2R, 3S. 5R. 1'R. 2'R !-1-t-Butoxycarbonyl-2-(1-acetamido-2-hydroxy-2- (thiophen-2-vl)) ethvl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic Acid t-Butyl Ester.

()- (2R, 3S, 5R, 1'R)-1-t-Butoxycarbonyl 2- (1-acetamido-2-formyl) ethyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (40 mg, 0.098 mmol) in THF (2 mL) was added dropwise to a solution of 2-thienyllithium (1 M in THF, 0.505 mmol, 5 equivalents) in THF (1 mL) at 25 °C and reacted for 20 minutes.

The reaction was quenched with saturated aqueous ammonium chloride (2 mL) and water (5 mL) followed by extraction using dichloromethane (2 X 10 mL). The organic layer was dried over magnesium sulfate, filtered and concentrated in vacuo. The residue was purified by column chromatography on silica gel using 1/1: ethyl acetate/hexane to provide the title compound (yield : 9.5 mg, 20%).

MS : (M+H) += 495, (M+Na) += 517, (M-H)-= 493.

73B (~)-(2R,3S,5R,1'R,2'S)-2-(1-Acetamido-2-hydroxy-2-(thiophen- 2-yl)ethyl- 3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41C, substituting ()- (2R, 3S, 5R, 1'R, 2'R)-1-t-butoxycarbonyl-2-(1- acetamido-2-hydroxy-2-(thiophen-2-yl)) ethyl-3-(cis-propen-1-yl)-pyrrolidine-5- carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl- 2- (1-acetamido-2-hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t- butyl ester (yield : 4.3 mg, 100%).

'H NMR (DMSO-d6) 8 7.86 (d, J= 9.8Hz, 1H), 7.63 (dd, J= 5.4,1.0 Hz, 1H), 7.07 (m, 1 H), 6.98 (m, 1 H), 5.58 (m, 1 H), 5.43 (m, 1 H), 4.55 (m, 1 H), 4.39 (m, 1 H), 3.72 (m, 1H), 3.11 (m, 2H), 2.43 (m, 1H), 2.04 (s, 3H), 1.80 (m, 1H), 1.57 (m, 3H).

MS: (M+H)+=339, (M+Na) + = 361, (M-H)-= 337.

Example 74 (~)-(2R,3S,5R,1'R,2'S)-2-(1-Acetamido-2-hydroxy-3-(4-methylt hiazol-2-yl))propyl- 3- (cis-propen-1-vI)-nyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 74A (2R,3S,5R,1'R, 2'S) and ftL (2R, 3S. 5R. 1'R, 2'R)-1-t-Butoxvcarbonyl-2- (1-Acetamido-2-hydroXv-3-(4-methylthiazol-2-yl)) proPyl-3-(cis-propen-1-yl)- pyrrolidine-5-carboxylic Acid t-Butyl Ester.

1.6 M n-Butyllithium (0.125 mL, 0.20 mmol, 4 equivalents) was added to a solution of 2, 4-dimethylthiazole (28.3 mg, 0. 25 mmol, 5 equivalents) in 1 mL of THF at-78 °C and reacted for 30 minutes. ( ()- (2R, 3S, 5R, 1'R)-1-t- <BR> <BR> <BR> <BR> butoxycarbonyl 2- (1-acetamido-2-formyl) ethyl-3- (cis-propen-1-yl)-pyrrolidine-5- carboxylic acid t-butyl ester (20.5 mg, 0.050 mmol) in THF (1 mL) was added dropwise to the above solution and reacted for 30 minutes at-78 °C and then for 30 minutes at room temperature. The reaction mixture was quenched with saturated aqueous ammonium chloride (5 mL) and water (5 mL) followed by extraction using dichloromethane (3 X 25 mL). The organic layer was dried over magnesium sulfate, filtered and concentrated in vacuo. The residue was purified by column chromatography on silica gel using 1/2: ethyl acetate/hexane to provide ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1-acetamido-2-hydroxy-3- (4-methylthiazol-2-yl)) propyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t- butyl ester (yield : 3.3 mg, 13%) and ()- (2R, 3S, 5R, 1'R, 2'R)-1-t-butoxycarbonyl- 2- (1-acetamido-2-hydroxy-3- (4-methylthiazol-2-yl)) propyl-3- (cis-propen-1-yl)- pyrrolidine-5-carboxylic acid t-butyl ester (yield : 7.5 mg, 29%).

(2R, 3S, 5R, 1'R, 2'S) MS: (M+H) +=524, (M+Na) +=546, (2M+Na) +=1069, (M- H)-=522.

(2R, 3S, 5R, 1'R, 2'R) MS: (M+H) +=524, (M+Na) +=546, (2M+Na) +=1069, (M- H)-=522.

74B ~)-(2R,3S,5R,1'R,2'S)-2-(1-Acetamido-2-hydroxy-3-(4-methylth iaxol-1- yl) propyycis-propen-1-yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41C, substituting ()- (2R, 3S, 5R, 1'R, 2'S)-1-f-butoxycarbonyl-2- (1- acetamido-2-hyd roxy-3- (4-methylthiazol-2-yl)) propyl-3- (cis-propen-1-yl)- pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t- butoxycarbonyl-2- (1-acetamido-2-hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5- carboxylic acid t-butyl ester (yield : 0.0030 g, 100%).

'H NMR (DMSO-d6) 8 9.0 (bs, 1H), 8.10 (d, J=8.3Hz, 1H), 7.11 (d, J=1.0 Hz, 1H), 5.48 (m, 1H), 5.30 (m, 1H), 4.30 (m, 1H), 4.10 (m, 1H), 3.88 (dt, J=9.4,2.6Hz, 1H), 3.78 (m, 1H), 3.25-3.15 (m, 2H), 2.93 (dd, J=15. 1,8.3Hz, 1H), 2.41 (dt, J=12.3,7.3Hz, 1H), 2.33 (d, J=1. 0Hz, 3H), 1.86 (s, 3H), 1.66 (dt, J=12.7, 10.3Hz, 1H), 1.61 (dd, J=6.8,1.5Hz, 3H).

MS: (M+H)+= 368, (M+Na) + = 390, (M-H)-= 366.

Example 75 (~)-(2R,3S, 5R. 1'R. 2R)-2-( 1-Acetamido-2-hydroxy-3- (4-methylthiazol-2-vl)) propyl- 3-(cis-propen-1-yl)-pyrroldine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41C, substituting ()- (2R, 3S, 5R, 1'R, 2'R)-1-t-butoxywarbonyl-2-(1- acetamido-2-hydroxy-3- (4-methylthiazol-2-yl)) propyl-3- (cis-propen-1-yl)- pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t- butoxycarbonyl-2- (1-acetamido-2-hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5- carboxylic acid t-butyl ester (yield : 0.0030 g, 100%).

1H NMR (DMSO-d6) d9.0 (bs, 1H), 7.77 (d, J=9.3Hz, 1H), 7.11 (s, 1H), 5.47 (m, 1H), 5.25 (m, 1H), 4.45 (m, 1H), 4.20 (m, 2H), 3.58 (t, J=9. 1Hz, 1H), 3.19 (m, 1H), 2.96 (m, 2H), 2.41 (m, 1H), 2.33 (d, J=1. 0Hz, 3H), 1.85 (s, 3H), 1.73 (dt, J=12. 7,10.3Hz, 1H), 1.54 (dd, J=6.9,1.5Hz, 3H).

MS: (M+H)+=368, (M+Na) + = 390, (M-H)-= 366, (M+CF3COOH)-=480, (2M-H)-=733.

Example 76 (t)- (2R. 3S. 5R. 1'R, 2'RS)-2-1-Acetamido-2-hydroxy-3- (thiazolin-2-vl)) propvl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 76A ()- (2R, 3S, 5R, 1'R, 2'RS)-1-t-Butoxycarbonyl-2- (1-Acetamido-2- hydroxy-3- (thiazolin-2-yl)) propyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic Acid t-Butyl Ester ()- (2R, 3S, 5R, 1'R)-1-t-Butoxycarbonyl 2- (1-acetamido-2-formyl) ethyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (20.5 mg, 0.05 mmol) in THF (1 mL) was added dropwise to a solution of the (thiazolin-2-yl) methyl lithium (0.20 mmol, 4 equivalents, prepared from 0.025 g of 2-methylthiazoline and 0.125 mL of 1.6 M n-BuLi at-78 °C) in THF (2 mL) at-78 °C and reacted for 30 minutes. The reaction was quenched with saturated aqueous ammonium chloride (5 mL) and water (5 mL) followed by extraction using dichloromethane (3 X 20 mL). The organic layer was dried over magnesium sulfate, filtered and concentrated in vacuo. The residue was purified by column chromatography on silica gel using 1/1 : ethyl acetate/hexane to provide the title compound as a mixture of isomers (yield : 10 mg, 40%).

MS: (M+H) += 512, (M+Na) +=534, (M-H)-=510.

76B (~)- (2R, 3S 5R. 1'R, 2'RS)-2-(1-Acetamido-2-hydroxy-3-(thiazolin-2- yl))l))propyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compounds were prepared according to the method described in Example 41C, substituting ()- (2R, 3S, 5R, 1'R, 2'RS)-1-t-butoxycarbonyl-2- (1- acetamido-2-hydroxy-3-(thiazolin-2-yl))propyl-3-(cis-propen- 1-yl)-pyrrolidine-5- carboxylic acid t-butyl ester n place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl- <BR> <BR> <BR> 2- (1-acetamido-2-hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t- butyl ester (yield : 0.003 g, 100%).

Major isomer 1H NMR (DMSO-d6) 8 8.88 (m, 1H), 7.76 (d, J=8.8Hz, 1H), 5.46 (m, 1H), 5.19 (m, 1 H), 4.69 (m, 1H), 3.90 (m, 1H), 3.85 (m, 1H), 3.49 (m, 2H), 3.35 (t, J=9. OHz, 1H), 3.29 (dd, J=17. 6,5.9Hz, 1H), 3.04 (t, J=8.9Hz, 1H), 2.78 (dd, J=17. 6,8. 1Hz, 1H), 2.7-2.55 (m, 2H), 1.75 (s, 3H), 1.70 (m, 1H), 1.56 (dd, J=6.8,1.5Hz, 3H).

MS: (M+H)+=356, (M+Na) +=378, (2M+Na) +=733, (M-H)-=354.

Example 77 (ffi)-(2R 3S 5R, 1'R 2'S)-2-(1-Acetamido-2-hvdroxy-3 3-difluoro-3-vinyl) pronvl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 77A (~)-(2R,3S,5R,1'R,2'S)-1-t-butoxycarbonyl-2-(1-acetamido-2-h ydroxy-3,3- difluoro-3-vinyl propyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic Acid t-Butvl Ester ()- (2R, 3S, 5R, 1'R)-1-t-Butoxycarbonyl 2- (1-acetamido-2-formyl) ethyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (41 mg, 0.10 mmol) and 1,1-difluoroallyl iodide (94 mg, 0.60 mmol, 6 equivalents) in THF (2 mL) was reacted with zinc dust (33 mg, 0.50 mmol, 5 equivalents) at 0°C for 5 minutes and then at room temperature for 4 hours. The reaction mixture was quenched with saturated aqueous ammonium chloride (15 mL) and water (15 mL) and extracted with 3 X 25 mL dichloromethane. The organic layer was dried over magnesium sulfate, filtered and concentrated in vacuo. The residue was purified by column chromatography on silica gel using 1/3 : ethyl acetate/hexane to provide the title compound (yield : 35 mg, 71%).

MS: (M+H) +=489, (M+Na) +=511, (2M+Na) +=999, (M-H)-=487.

77B (t- (2R, 3S, 5R, 1'R. 2'S)-2- 1-Acetamido-2-hvdroxy-3. 3-difluoro-3- vinv!)DroDvt-3-c/s-Dropen-1-v!)-Dvrro)idine-5-carboxv)ic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41 C, substituting (~)-(2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- acetamido-2-hydroxy-3,3-difluoro-3-vinyl) propyl-3- (cis-propen-1-yl)-pyrrolidine-5- carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl- 2-(1-acetamido-2-hydroxy)butyl-3-(cis-propen-1-yl)-pyrrolidi ne-5-carboxylic acid t- butyl ester (yield : 0.0026 g, 96%).

'H NMR (DMSO-d6) d7.68 (d, J=7.8Hz, 1 H), 5.97 (m, 1 H), 5.55-5.45 (m, 2H), 5.43 (m, 1H), 5.23 (m, 1H), 4.45 (m, 2H), 4.10 (m, 1H), 3.16 (quint. J=9. 1Hz, 1 H), 2.41 (dt, J=12. 8,7.3Hz, 1H), 1.72 (s, 3H), 1.70 (dt, J=12. 8,10.3Hz, 1H), 1.61 (dd, J=6.7,1.2Hz, 3H).

MS: (M+H) +=333, (M+Na) +=355, (M-H)-= 331, (2M-H)-=663.

Example 78 (~)-(2R,3S,5R,1'R,2'S)-2-(1-Acetamido-2-hydroxy-3,3-difluoro -3-vinyl)propyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 78A (iM2R. 3S, 5R. 1'R)-1-t-Butoxycarbonyl-2- (1-Acetamido-2-oxo-3, 3-difluoro- 3-vinyl) propyl-3- (cis-propen-1-vl)-pyrrolidine-5-carboxylic Acid t-Butvl Ester.

The title compound was prepared according to the method described in Example 42A, substituting (i)-(2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- <BR> <BR> <BR> acetamid o-2-hyd roxy-3, 3-d ifl uoro-3-vinyl) propyl-3- (cis-propen-1-yl)-pyrrolidine-5- carboxylic acid t-butyl ester in place of (2R, 3S, 5R, 1'R, 2'R)-2- (1-acetamido-2- hydroxy)butyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester to provide ()- (2R, 3S, 5R, 1'R)-1-t-butoxycarbonyl-2- (1-acetamido-2-oxo-3, 3-difluoro- 3, 3-difluoro-3-vinyl) propyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 0.0050g, 44%).

MS: (M+H) +=487, (M+Na) +=509, (M-2F) +=448, (M-H)-=485.

78B(t)- (2R, 3S. 5R, 1'R. 2'R)-1-t-Butoxvcarbonyl-2- 1-Acetamido-2-hydroxy-3. 3- difluoro-3-vinylZpropv,- (cis-propen-1-yl)-pvrrolidine-5-carboxylic Acid t-Butvl Ester.

The title compound is prepared according to the method described in Example 42B, substituting ()- (2R, 3S, 5R, 1'R)-1-t-butoxycarbonyl-2-(1-acetamido- 2-oxo-3-difluoro-3-vinyl) propyl-3-(cis-propen-1-yl)pyrrolidine-5-carboxylic acid t butyl ester in place of (2R, 3S, 5R, 1'R)-2- (1-acetamido-2-oxo) butyl-3- (cis-propen- 1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester.

78C (~)-(2R,3S,5R,1'R,2'R)-2-(1-Acetamido-2-hydroxy-3,3-difluoro -3- vinyl) propyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxvlic Acid Trifluoroacetic Acid The title compound is prepared according to the method described in Example 41C, substituting ()- (2R, 3S, 5R, 1'R, 2'R-1-t-butoxycarbonyl-2-(1- acetamido-2-hydroxy-3, 3-difluoro-3-vinyl) propyl-3-(cis-propen-1-yl)-pyrrolidine-5- carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl- <BR> <BR> <BR> <BR> 2- (1-acetamido-2-hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t- butyl ester.

Example 79 (~)-(2R,3S,5R,1'R,2'S)-2-(1-Acetamido-2-hydroxy-2-(cis-buten -2-yl)ethyl-3-(cis- pronen-1-yl)-nyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 79A (~)-(2R,3S,5R,1'R,2'S)-1-t-Butoxycarbonyl-2-(1-Acetamido-2-h ydroxy-2- (cis-buten-2-yl)ethyl-3-(cis-propen-1-yl)-pyrrolidine-5-carb oxylic Acid t-Butyl Ester.

()- (2R, 3S, 5R, 2'R)-1-t-Butoxycarbonyl 2- (1-acetamido-2-formyl) ethyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (30 mg, 0.073 mmol) in THF (5 mL) was reacted with cis-2-buten-2-yl lithium (0.75 mL (0.5M), 0.37 mmol) at 25°C for 45 min. The reaction was quenched with saturated aqueous ammonium chloride (5 mL) and water (5mL) followed by extraction using dichloromethane (2 X 10 mL). The organic layer was dried over magnesium sulfate, filtered and concentrated in vacuo. The residue was purified by column chromatography on silica gel using 1/1 : ethyl acetate/hexane to provide the title compound (yield : 20 mg, 59%).

'H NMR (CDC13) 8 6.19 (d, J=8.9 Hz, 1H), 5.61 (m, 1 H), 5.35 (m, 1H), 5.27 (m, 1 H), 4.48 (m, 1 H), 4.18 (m, 1 H), 4.77 (m, 2H), 3.10 (m, 1 H), 2.72 (m, 1 H), 1.99 (s, 3H), 1.82 (m, 1H), 1.73 (m, 3H), 1.55 (m, 6H), 1.47 (s, 9H), 1.44 (s, 9H) MS : (M+H) += 467, (M-H)-= 465 79BfiM2R.3S.5R.1'R.2'R)-2-n-Acetamido-2-hvdroxv-2-(c/s-buten -2-v!ethv!-<BR> <BR> <BR> <BR> <BR> 3- !-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41 C, substituting ()- (2R, 3S, 5R, 1'R, 2'R)-1-t-butoxycarbonyl-2-(1- acetamido-2-hydroxy-2-(cis-buten-2-yl)) ethyl-3-(cis-propen-1-yl)-pyrrolidine-5- carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl- 2-(1-acetamido-2-hydroxy)butyl-3-(cis-propen-1-yl)-pyrrolidi ne-5-carboxylic acid t- butyl ester (yield : 4 mg, 96%).

1H NMR (DMSO-d6) 8 8.09 (d, J=9.0 Hz, 1H), 5.50 (m, 1H), 5.32 (m, 1H), 5.16 (m, 1 H), 4.50 (m, 1H), 4.38 (m, 1H), 4.19 (m, 1H), 3.43 (m, 1H), 3.20 (m, 1H), 2.43 (m, 1H), 1.88 (s, 3H), 1.74 (m, 1H), 1.70 (s, 3H), 1.62 (m, 3H), 1.58 (m, 3H) MS: (M+H)+=311, (M-H)-=309 Example 80 (~)-(2R,3S,5R,1'R,2'R, 3'R) and (t)- (2R, 3S, 5R, 1'R, 2'R. 3'S)-2- 1-Acetamido-2- hydroxy-3-methyl)pentyl-3-(cis-propent-1-yl)-pyrrolidine-5-c arboxylic Acid Trifluoroacetic Acid Salt 80A (i)-(2R, 3S, 5R, 1'R, 2'R, 3'R) and (i)-(2R, 3S, 5R, 1'R, 2'R. 3'S)-1-t- <BR> <BR> <BR> Butoxycarbonvl-2-(1-acetamido-2-hvd roxy-3-methyl) nentyl-3-(cis-propen-1-yl !-<BR> <BR> <BR> <BR> <BR> pyrrolidine-5-carboxylic Acid t-Buyl Ester.

()- (2R, 3S, 5R, 1'R)-1-t-Butoxycarbonyl 2-(1-acetamido-1-formyl) methyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (60 mg, 0.15 mmol) in THF (1 mL) was added dropwise to a solution of 2-butylmagnesium bromide (3M in ether) (0.45 mL, 0.85 mmol) at room temperature and reacted for 40 minutes.

The reaction was quenched with saturated NH4CI (1 mL) followed by extraction using dichloromethane (3 x 1 mL). The organic layer was dried over magnesium sulfate, filtered and concentrated in vacuo. The residue was purified by column chromatography on silica gel using 1/4: ethyl acetate/hexane to provide the title compounds ()- (2R, 3S, 5R, 1'R, 2'R, 3'S)-t-butoxycarbonyl 2-(1-acetamido-2- hydroxy-3-methyl) pentyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester Rf= 0.65 (1: 1 ethyl acetate: hexanes) (yeld: 19 mg, 27%) and ()- (2R, 3S, 5R, 1'R, 2'R, 2'R)-1-t-butoxycarbonyl-2-(1-acetamido-2-hydrocy-3- methyl)pentyl-3-(cis-propen-1-yl)pyrrolidine-5-carboxylic acid t-butyl ester Rf= 0.5) (1 : 1 ethyl acetate : hexanes) (yield : 19 mg, 27%).

Rf= 0. 65 1H NMR (CDC13) 6 5.98 (d, J=8.8Hz, 1H), 5.62 (t, J=10. 5Hz, 1H), 5.35 (m, 1H), 4.66 (d, J=4.4Hz, 1H), 4.16 (d, J=9.5Hz, 1H), 3.78 (m, 3H), 3.12 (m, 2H), 2.73 (m, 1 H), 2.0 (s, 3H), 1.81 (d, J=13. 2Hz, 1 H), 1.54 (br s, 3H), 1.47 (s, 9H), 1.44 (s, 9H), 1.25 (m, 1H), 0.81 (m, 6H) MS: (M-H)-= 467; (M+H) + = 469.

Rf= 0. 5'H NMR (CDC13) 8 6. 00 (d, J=10. 2Hz, 1H), 5.61 (brt, 1H), 5.36 (m, 1H), 4.58 (d, J=4.7Hz, 1H), 4.14 (d, J=8.8Hz, 1 H), 3.82 (m, 3H), 3.13 (m, 2H), 2.73 (m, 1 H), 1.99 (s, 3H), 1.80 (d, J=13. 9Hz, 1H), 1.54 (br s, 3H), 1.46 (s, 9H), 1.44 (s, 9H), 1.43 (m, 1H), 0.97 (d, J=6.8Hz, 3H), 0.81 (t, J=7.2Hz, 3H) MS: (M-H)'= 467; (M+H) + = 469.

80B (~)-(2R,3S,5R,1'R,2'S)-2-(1-Acetamido-2-hydroxy-3-methyl)pen tyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt ()- (2R, 3S, 5R, 1'R, 2'R, 2'S)-1-t-Butoxycarbonyl-2-(1-acetamido-2-hydroxy- 3-methyl)pentyl-3-(cis -propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (2.5 mg, 0.005 mmol) was reacted with trifluoroacetic acid (0.8 mL) in dichloromethane (0.2 mL) at room temperature for 6 hrs. The reaction was concentrated in vacuo overnight and triturated with acetonitrile (2 x 1 mL) to provide the title compound (yield : 2.0 mg, 100%).

1 H NMR- (DMSO-d6) 8 7 : 68 (d, J=8. 8Hz, 1H), 5.45 (m, 1 H), 5.23 (t, J=7.3Hz, 1 H), 4.24 (br t, 1 H), 4.18 (m, 1 H), 3.52 (t, J=7.3Hz, 1 H), 3.45 (m, 1 H), 3.16 (m, 1H), 2.38 (m, 1 H), 1.83 (s, 3H), 1.68 (m, 1H). 1.58 (dd, J=2.0,4.8Hz, 3H), 1.37 (m, 2H), 0.99 (m, 1 H), 0.89 (d, J=6. 8Hz, 3H), 0.79 (t, J=7.4Hz, 3H) MS: (M-H)-= 311 ; (M+H) + = 313, (M+Na) + = 335.

Example 81 ()- (2R, 3S, 5R. 1'R. 2'R. 2'R)-2-(1Acetamido-2-hydroxy-3-methyl)pentyo-3-(cis- propen-1-yl)-pyrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41C, substituting ()- (2R, 3S, 5R, 1'R, 2'R, 3'R)-1-t-butoxycarbonyl-2-(1- acetamido-2-hydroxy-3-methyl)pentyl-3-(cis-propent-1-yl)-pyr rolidine-5-carboxylic acid t-butyl ester (Rf= 0.5,1: 1, ethyl acetate: hexanes) in place of ()- (2 R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1-acetamido-2-hydroxy) butyl-3-(cis- propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 1.6 mg, 76%).

1H NMR (DMSO-d6) 8 7.55 (d, J=9.3Hz, 1H), 5.45 (m, 1H), 5.23 (m, 1H), 4.31 (br t, 1 H), 4.20 (t, J=8.3Hz, 1 H), 3.51 (t, J=9.3Hz, 1 H), 3.43 (d, J=7.4Hz, 1H), 3.17 (m, 1H), 2.40 (m, 1H), 1.80 (s, 3H), 1.70 (m, 1H), 1.55 (dd, J=1.4, 5.4Hz, 3H), 1.36 (m, 2H), 1.14 (m, 1H), 0.84 (t, J=7.3Hz, 3H), 0.73 (d, J=6.9Hz, 3H) MS: (M-H)-= 311 ; (M+H)+=313, (M+Na)+= 335.

Example 82 <BR> <BR> <BR> ()-(2R.3S.5R.1'R.2'S.3'S)-2-n-Acetamido-2-hvdroxv-3-methv))D entv!-3-(c/s- propen-1-yl)-pvrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt.

82A (2R, 3S. 5R. 1'R. 3'RS)-1-t-Butoxycarbonyl-2- (1-acetamido-2-oxo-3- methyl)pentyl-3-(cis-propen-1-yl)-pyrrolidine-5-cabroxylic Acid t-Butyl Ester.

The title compound was prepared according to the method described in Example 42A, substituting ()- (2R, 3S, 5R, 1'R, 2'R, 3'RS)-1-t-butoxycarbonyl-2- (1- acetamido-2-hydroxy-3-methyl)pentyl-3-(cis-propent-1-yl)-pyr rolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'R) 1-t-butoxycarbonyl-2-(1- acetamido-2-hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl (yield : 12 mg, 63%).

82B (i)-(2R, 3S, 5R, 1'R, 2'S, 3'S) and (~)-(2R, 3S, 5R, 1'R, 2'S, 3'R)-1-t- Butoxvcarbonvl-2-(1-acetamido-2-hydroxy-3-methyl)pentyl-3-(c is-propen-1-yl)- pyrrolidine-5-carboxylic Acid t-Butyl Ester.

The title compounds were prepared according to the method described in Example 42B, substituting ()- (2R, 3S, 5R, 1'R, 3'Rs)-1-t-butoxycarbonyl-2-(1- acetamido-2-oxo-3-methyl) pentyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (Rf= 0.5 and 0.65,1: 1, ethyl acetate: hexanes) in place of (2R, 3S, 5R, 1'R)-2-(1-acetamido-2-oxo) butyl-3-(cis-propen-1-yl)-pyrrolidine-5- carboxylic acid t-butyl ester to give ()- (2R, 3S, 5R, 1'R, 2'S, 3'S)-1-t- <BR> <BR> <BR> butoxycarbonyl-2- (1-acetamido-2-hydroxy-3-methyl) pentyl-3- (cis-propen-1-yl)- pyrrolidine-5-carboxylic acid t-butyl ester (Rf= 0.15,1: 1, ethyl acetate: hexanes) (yield : 6.0 mg, 50%) and ()- (2R, 3S, 5R, 1'R, 2'S, 3'R)-1-t-butoxycarbonyl-2-(1- acetamido-2-hydroxy-3-methyl)pentyl-3-(cis-propen-1-yl)-pyrr olidine-5-carvoxylic acid t-butyl ester (Rf= 0.10,1: 1, ethyl acetate: hexanes) (yield : 2.5 mg, 63%).

82C ()- (2R. 3S. 5R. 1'R, 2'S. 3'S)-2- (1-Acetamido-2-hydroxv-3-methyl) pentyl-3- (cis-propent-1-yl)-pyrroldine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41C, substituting ()- (2R, 3S, 5R, 1'R, 2'S, 3'S)-1-t-butoxycarbonyl-2-(1- acetamido-2-hydroxy-3-methyl)pentyl-3-(cis-propen-1-yl)-pyrr olidine-5-carboxylic acid t-butyl ester (Rf= 0.15,1: 1, ethyl acetate: hexanes) in place of (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1-acetamido-2-hydroxy)butyl-3-(ci s- propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 6.0 mg, 100%).

'H NMR (DMSO-d6) 8 7.78 (d, J=9.2Hz, 1H), 5.42 (m, 1H), 5.29 (t, J=10.3Hz, 1H), 4.08 (m, 1H), 3.96 (br t, 1H), 3.51 (m, 2H), 3.08 (m, 1H), 2.33 (m, 1H), 1.78 (s, 3H), 1.56 (d, J=6.3Hz, 3H), 1.52 (m, 1 H), 1.40 (m, 1H), 1.29 (m, 1H), 1.21 (m, 1H), 0.84 (t, J=7.3Hz, 3H), 0.73 (d, J=6.9Hz, 3H) MS: (M-H)-= 311 ; (M+H) + = 313, (M+Na) + = 335.

Example 83 (~)-(2R,3S,5R,1'R,2'S,3'R)-2-(1-Acetamido-2-hydroxy-3-methyl )penytyl-3-(cis- propen-1-yl-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41C, substituting ()- (2R, 3S, 5R, 1'R, 2'S, 3'R)-1-t-butoxycarbonyl-2-(1- acetamido-2-hydroxy-3-methyl) pentyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (Rf= 0.10,1: 1 ethyl acetate: hexanes) in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2- (1-acetamido-2-hydroxy) butyl-3- (cis- propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 2.5 mg, 100%).

'H NMR (DMSO-d6) 8 7.85 (d, J=8.7Hz, 1 H), 5.45 (m, 1 H), 5.29 (t, J=9.3Hz, 1 H), 4.20 (m, 2H), 3.63 (t, J=8. 3Hz, 1 H), 3.42 (br d, 1 H), 3.14 (m, 1 H), 2.41 (m, 1H), 1.79 (s, 3H), 1.62 (m, 1H), 1.58 (d, J=5.4Hz, 3H), 1.43 (m, 2H), 1.0 (m, 1H), 0.88 (d, J=6.8Hz, 3H), 0.80 (t, J=7.3Hz, 3H) MS : (M-H)-= 311 ; (M+H) + = 313, (M+Na) + = 335.

Example 84 (~)-(2R,3S,5R 1'R. 2'S)-2-(1-Acetamido-2-methoxy) butyl-3-(cis-propen-1-vl)- pyrrolidine-5-carboxYlic Acid Trifluoroacetic Acid Salt 84A(i)-(2R, 3S, 5R, 1'R, 2'S)-1-t-Butoxycarbonyl-2-(1-acetamido-2-<BR> <BR> <BR> methoxy)butyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic Acid t-Butyl Ester ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-Butoxycarbonyl-2-(1-acetamido-2- hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid f-butyl ester (22 mg, 0.05 mmole) was reacted with methyl iodide (0.016 mL, 0.25 mmole), potassium hydroxide (14 mg, 0.25 mmole) and 18-crown-6 (0.7 mg, 0.0025 mmole) in N, N-dimethylformamide (2 mL) at room temperature for 23 hours.

Water (5 mL) was then added to the reaction mixture, followed by extraction with ether (2 x 10 mL). The organic layer was washed with water, and brine, dried over MgSO4, filtered and concentrated in vacuo. The residue was purified by chromatography on silica gel using 66% ethyl acetate/hexanes to provide the title compound, as a colorless oil (yield : 5.2 mg, 23%).

MS: (M+H) += 455, (M-H)-= 453.

84B ~)-(2R,3S,5R,1'R,2'S,3'R)-2-(1-Acetamido-2-hydroxy-3-methyl) penytyl-3-(cis- propen-1- yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41 C, substituting ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxywarbonyl-2-(1- acetamido-2-methoxy)butyl-3-(cis-propen-1-yl)-pyrrolidine-5- carboxylic acid t- butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1-acetamido- 2-hydroxy)-butyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxyli c acid t-butyl ester (yield : 4.7 mg, 98%).

1H NMR (DMSO-d6) 8 7.96 (d, J= 9.2Hz, 1H), 5.50 (m, 1H), 5.24 (m, 1H), 4.25 (m, 2H), 3.70 (m, 1H), 3.23 (s, 3H), 3.19 (m, 2H), 2.40 (m, 2H), 1.86 (s, 3H), 1.68 (m, 2H), 1.62 (dd, J= 7.0,1.8Hz, 3H), 1.39 (m, 1 H), 0.77 (t, J= 7.3Hz, 3H).

MS: (M+H) += 299, (M+Na) += 321, (M-H)-= 297 Example 85 (~)-(2R,3S, 5R. 1'R. 2'R)-2-(1-Acetamido-2-methoxy) butvl-3-(cis-nroPen-1-yl)- pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 85A (~)-(2R,3S,5R,1'R,2'S,2'R)-1-t-Butoxycarbonyl-2-(1-Acetamido -2- methoxy) butyl-3-(cis-propen-1-yl)-pYrrolidine-5-carboxylic Acid t-Butyl Ester ()- (2R, 3S, 5R, 1'R, 2'R)-1-t-Butoxycarbonyl-2- (1-acetamido-2- hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (17 mg, 0.04 mmole) was reacted with methyl iodide (28 mg, 0.19 mmole), potassium hydroxide (8 mg, 0.19 mmole) and 18-crown-6 (0.002 mmole) in N, N- dimethylformamide (1.5 mL) at room temperature for 6 hours. Water (5 mL) was then added to the reaction mixture, followed by extraction with ether (2 x 10 mL).

The organic layer was washed with water, and brine, dried over MgS04, filtered and concentrated in vacuo. The residue was purified by chromatography on silica gel using 50% ethyl acetate/hexanes to provide the title compound, (yield : 5 mg, 29%).

MS: (M+H) +=455, (M-H)-=453 85B (~)-(2R,3S,5R,1'R,2'S,2'R)-2-(1-Acetamido-2-methoxy)butyl-3- (cis-propen-1- yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41C, substituting ()- (2R, 3S, 5R, 1'R, 2'R)-1-t-butoxycarbonyl-2-(1- acetamido-2-methoxy)butyl-3-(cis-propen-1-yl)-pyrrolidine-5- carboxylic acid t- butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxywarbonyl-2-(1-acetamido- 2-hydroxy)butyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 4 mg, 95%).

1H NMR (DMSO-d6) d 8.00 (d, J=9.8HZ, 1H), 5.57 (m, 1H), 5.35 (m, 1H), 4.42 (m, 1H), 4.28 (m, 1H), 3.95 (m, 1H), 3.54 (m, 1H), 3.28 (s, 3H), 2.80 (m, 1H), 2.30 (m, 1H), 1.92 (s, 3H), 1.65 (m, 1H), 1.60 (m, 3H), 1.43 (m, 2H), 0.82 (t, J=7. 31Hz, 3H).

MS: (M+H) +=299, (M-H)-=297 Example 86 (~)-(2R,3S,5R,1'R,2'S,)-2-(1-Acetamido-2-methoxy-3-methyl)-b utyl-3-(cis-propen- 1-yl)-pyrrolidine-5-carboxvlic Acid Trifluoroacetic Acid Salt 86A (~)-(2R,3S,5R,1'R,2'S,)-1-t-Butoxycarbonyl-2-(1-Acetamido-2- methoxy-3- methyl) butyl-3- (cis-propen-1-yl)-pvrrolidine-5-carboxylic Acid t-Butyl Ester The title compound is prepared according to the method described in Example 84A, substituting ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- acetamido-2-hydroxy-3-methyl)butyl-3-(cis-propen-1-yl)-pyrro lidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- <BR> <BR> <BR> acetamido-2-hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester.

86B (~)-(2R,3S,5R,1'R,2'S,)-2-(1-Acetamido-2-methoxy-3-methyl)bu tyl-3-(cis- propen-1-yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound is prepared according to the method described in Example 41C, substituting ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- acetamido-2-methoxy-3-methyl) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2- (1- acetamido-2-hydroxy) butyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester.

Example 87 ()- (2R. 3S. 5R, 1'R. 2'R)-2- 1-Acetamido-2-methoxy-3-methvl) butyl-3- (cis-propen-<BR> <BR> 1-yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 86A (~)-(2R, 3S. 5R. 1'R. 2'R)-1-t- (1-acetamido-2-methoxv-3- methyl)butyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic Acid t-Butyl-cster The title compound was prepared according to the method described in Example 84A, substituting ()- (2R, 3S, 5R, 1'R, 2'R)-1-t-butoxycarbonyl-2-(1- acetamido-2-hydroxy-3-methyl)butyl-3-(cis-propen-1-yl)-pyrro lidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- acetamido-2-hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 6.8 mg, 33%).

MS : (M+H)+=469, (M+Na) += 491, (M-H)-= 467.

87B (~)-(2R,3S,5R, 1'R. 2'R)-2- (1-Acetamido-2-methoxy-3-methvl) butyl-3- (cis- propen-1-yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41 C, substituting ()- (2R, 3S, 5R, 1'R, 2'R)-1-t-butoxycarbonyl-2-1- acetamido-2-methoxy-3-methyl) buyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- acetamido-2-hyroxy)butyl-3-(cis-propen-1-yl)pyrrolidine-5-ca rboxylic acid t-butyl ester. ester (yield : 6.6 mg, 100%).

1H NMR (DMSO-d6) 8 7.65 (d, J= 9.2Hz, 1H), 5.43 (m, 1H), 5.23 (m, 1H), 4.42 (m, 1H), 4.37 (m, 1H), 3.56 (m, 1H), 3.46 (s, 3H), 3.17 (m, 2H), 2.44 (m, 1H), 1.80 s, 3H), 1.78 (m, 1 H), 1.70 (m, 1 H), 1.57 (dd, J= 6.7,1.2Hz, 3H), 0.94 (d, J= 6.7Hz, 3H), 0.82 (d, J= 6.7Hz, 3H).

MS: (M+H) += 313, (M+Na) + = 335, (M-H)-= 311.

Example 88 (~)-(2R,2S, 5R. 1'R. 2'S)-2-(1-Acetamido-2-methoxv)Dentv)-3-(c/s-DroDen-1-v))- pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 88A (i)-(2R. 3S. 5R. 1'R. 2'S)-1-t-Butoxecarbonyl-2-(1-acetamido-2- methyoxy)pentyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic Acid t-Butyl Ester The title compound was prepared according to the method described in Example 84A, substituting ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxywarbonyl-2-(1- acetamido-2-hydroxy) pentyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t- butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1-acetamido- 2-hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 11.9 mg, 36%).

MS: (M+H)+=469, (M+Na) += 491, (M-H)-= 467.

88B (~)-(2R,3S,5R,1'R,2'S,)-2-(1-Acetamido-2-methoxy)pentyl-3-(c is-propen-1- yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41C, substituting ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- acetamido-2-methyoxy)pentyl-3-(cis-propen-1-yl)-pyrroidine-5 carboxylic acid t- butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1-acetamido- 2-hydroxy)butyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester.

(yield : 11.5 mg, 100%).

'H NMR (DMSO-d6) 8 7.95 (d, J= 9.8Hz, 1H), 5.49 (m, 1H), 5.23 (m, 1H), 4.25 (m, 2H), 3.68 (m, 1 H), 3.24 (s, 3H), 3.22 (m, 1 H), 3.18 (m, 1 H), 2.40 (m, 1 H), 1.85 (s, 3H), 1.66 (m, 1H), 1.62 (m, 3H), 1.58 (m, 1H), 1.38 (m, 1H), 1.27 (m, 2H), 0.86 (t, J= 7.3Hz, 3H).

MS: (M+H)+= 313, (M+Na) +=335, (M-H)-= 311.

Example 89 (~)-(2R,3S,5R,1'R,2'S,)-2-(1-Acetamido-2-methoxy)pentyl-3-(c is-propen-1-yl)- pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 89A(i)-(2R. 3S, 5R, 1'R. 2'R)-1-t-Butoxycarbonyl-2-(1-acetamido-2- methoxv) pentyl-3- (cis-propen-1-yl-pvrrolidine-5-carboxylic Acid t-Butyl Ester The title compound was prepared according to the method described in Example 84A, substituting ()- (2R, 3S, 5R, 1'R, 2'R)-1-t-butoxycarbonyl-2-(1- acetamido-2-hyd roxy) pentyl-3-(cis-propen-1-yl)-pyrrolid ine-5-carboxylic acid t- butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1-acetamido- 2-hydroxy)butyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield: 4.3 mg, 21%).

MS: (M+H)+= 469, (M+Na) += 491, (M-H)-= 467.

89B (~)-(2R,3S,5R,1'R,2'S,)-2-(1-Acetamido-2-methoxy)pentyl-3-(c is-propen-1- yl)-pvrrolidine-5-carboxvlic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41 C, substituting ()- (2R, 3S, 5R, 1'R, 2'R)-1-t-butoxywarbonyl-2-(1- acetamido-2-methyoxy)pentyl-3-(cis-propen-1-yl)-pyrrolidine- 5-ca5rboxylic acid t- butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1-acetamido- 2-hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 4.8 mg, 100%).

'H NMR (DMSO-d6) # 7.70 (d, J= 9.8Hz, 1H), 5.45 (m, 1H), 5.24 (m, 1H), 4.40 (m, 1H), 4.25 (m, 1H), 3.57 (t, J= 8.5Hz, 1H), 3.40 (m, 1H), 3.35 (s, 3H), 3.17 (m, 1H), 2.42 (m, 1H), 1.82 (s, 3H), 1.69 (m, 1H), 1.56 (dd, J= 7.1,1.2Hz, 3H), 1.24 (m, 4H), 0.88 (t, J= 7. OHz, 3H).

MS : (M+H) += 313, (M+Na) += 335, (M-H)-= 311 Example 90 (~)-(2R,3S,5R,1'R,2'S,)-2-(1-Acetamido-2-methoxy-2-allyl)eth yl-3-(cis-propen-1- yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 90A (~)-(2R,3S,5R,1'R,2'S,)-1-t-Butoxycarbonyl-2-(1-Acetamido-2- methoxy-2- allvl) ethyl-3- (cis-propen-1-y-pvrrolidine-5-carboxvlic Acid t-Butyl Ester The title compound was prepared according to the method described in Example 84A, substituting ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- <BR> <BR> acetamido-2-hydroxy-2-allyl) ethyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- <BR> <BR> <BR> acetamido-2-hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 8 mg, 31 %).

MS: (M+H) +=467, (M-H)-=465 90B (~)-(2R,3S,5R,1'R,2'S,)-2-(1-Acetamido-2-methoxy-2-allyl)eth yl-3-(cis- propen-1-yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41C, substituting ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2- (1- acetamido-2-methoxy-2-allyl) ethyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- acetamido-2-hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester. ester (yield : 6 mg, 96%).

'H NMR (DMSO-d6) 8 8.02 (d, J=8.6HZ, 1H), 5.75 (m, 1H), 5.51 (m, 1H), 5.24 (m, 1 H), 5.05 (m, 2H), 4.27 (m, 1 H), 4.22 (m, 1 H), 3.74 (m, 2H), 3.26 (s, 3H), 3.18 (m, 1 H), 2.47 (m, 1 H), 2.39 (m, 1 H), 2.17 (m, 1 H), 1.87 (s, 3H), 1.67 (m, 1H), 1.63 (dd, J=6.71,1.23 HZ, 3H).

MS: (M+H) +=311, (M-H)-=309 Example 91 (~)-(2R,3S,5R, 1'R. 2')-2-(1-Acetamido-2-methoxy-2-allyl)ethyl-3-(cis-propen-1- yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 91A (~)-(2R, 3S. 5R. 1'R. 2'R)-1-t-Butoxycarbonyl-2-(1-acetamido-2-methoxy-2- all ) ethyl-3- (cis-propen-1-vl)-pyrrolidine-5-carboxylic Acid t-Bu l Ester The title compound was prepared according to the method described in Example 84A, substituting ()- (2R, 3S, 5R, 1'R, 2'R)-1-t-butoxycarbonyl-2-(1- acetamido-2-hydroxy-2-allyl) ethyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester in place of (i)-(2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- acetamido-2-hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 4.0 mg, 16%).

MS: (M+H)+=467, (M-H)-=465 91B (~)-(2R 3S. 5R, 1'R. 2'R)-2-(1-Acetamido-2-methoxy-2-allyl)ethyl-3-(cis- propen-1-yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41 C, substituting ()- (2R, 3S, 5R, 1'R, 2'R)-1-t-butoxycarbonyl-2-(1- acetamido-2-methyoxy-2-allyl)ethyl-3-(cis-propen-1-yl)-pyrro lidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- acetamido-2-hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 3 mg, 96%).

1H NMR (DMSO-d6) 8 7.75 (d, J=9.2 HZ, 1H), 5.75 (m, 1H), 5.47 (m, 1H), 5.24(m, 1 H), 5.06 (m, 2H), 4.42 (m, 1H), 4.25 (m, 1H), 3.58 (m, 1H), 3.50 (m, 1H), 3.37 (s, 3H), 3.17 (m, 1H), 2.42 (m, 1H), 2.36 (m, 1H), 1.83 (s, 3H), 1.71 (m, 1H), 1.55 (dd, J=6.73,1.83 HZ, 3H) MS : (M+H) +=311, (M-H)-=309 Example 92 (~)-(2R,3S,5R,1'R,2'S,)-2-(1-Acetamido-2-hydroxy-4-vinyl)but yl-3-(bis-propen-1- vl)-pvrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt.

92A (i)-(2R, 3S, 5R, 1'R, 2'S) and (~)-(2R,3S, 5R. 1'R, 2'R)-1-t-Butoxycarbonyl-2- (1-acetamido-2-hydroxy-4-vinyl)-butyl-3-(cis-propen-1-yl)-py rrolidine-5-carboxylic Acidt-Butyl Ester.

The title compounds were prepared according to the method described in Example 41 B, substituting 1-buten-4-yl magnesium bromide for ethyl magnesium bromide to provide (i)-(2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2- (1-acetamido-2- hydroxy-4-vinyl) butyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 0.0030 g, 6%) and ()- (2R, 3S, 5R, 1'R, 2'R)-1-t-butoxycarbonyl-2-(1- acetamido-2-hydroxy-4-vinyl) butyl-3-(cis-propen-1-yl)-pyrrolid ine-5-carboxylic acid t-butyl ester (yield : 0.0145 g, 28%).

()- (2R, 3S, 5R, 1'R, 2'S) MS: (M+H) +=467, (M+Na) +=489, (2M+Na) +=955, (M-H)-=465.

()- (2R, 3S, 5R, 1'R, 2'R)- MS : (M+H) +=467, (M+Na) +=489, (2M+Na) +=955, (M-H)-=465.

92B (~)-(2R,3S,5R,1'R,2'S,)-2-(1-Acetamido-2-hydroxy-4-vinyl)but yl-3-(cis- propen-1-yl)-pyrrolidine-5-carboxvlic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41C, substituting ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2- (1- acetamido-2-hydroxy4-vinyl) butyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- acetamido-2-hydroxy) butyl-3-(cix-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 0.0027 g, 100%).

'H NMR (DMSO-d6) 8 8.93 (bs, 1H), 7.90 (d, J=9.2 Hz, 1H), 5.80 (m, 1H), 5.48 (m, 1H), 5.28 (m, 1H), 5.00 (dd, J=17. 1,1.8Hz, 1H), 4.94 (dd, J=10. 4,1.8Hz, 1H), 4.29 (bt, J=8.3Hz, 1H), 4.03 (m, 1H), 3.71 (m, 1H), 3.49 (m, 1H), 3.15 (quint., J=8.5Hz, 1H), 2.41 (dt, J=12.8,7.3Hz, 1H), 2.16 (M, 1H), 2.05 (m, 1H), 1.83 (s, 3H), 1.79-1.75 (m, 1H), 1.64 (m, 1H), 1.58 (dd, J=6.7,1.8Hz, 3H), 1.34 (m, 2H).

MS: (M+H) +=311, (M+Na)+=333, (M-H)-=309, (M+CF3COO-)-=423 Example 93 (~)-(2R,3S,5R, 1'R. 2'R)-2-(1-Acetamido-2-hydroxv4-vinyl) butyl-3-(cis-propen-1- yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt.

93A (~)-(2R, 3S. 5R. 1'R. 2'R)-1-(1-Acetamido-2-hydroxy-4-vinyl)-butyl-3-(cis- propen-1-yl)-pyrroldine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41 C, substituting ()- (2R, 3S, 5R, 1'R, 2'R)-1-t-butoxycarbonyl-2-(1- acetamido-2-hydroxy-4-vinyl)butyl-3-(cis-propen-1-yl)-pyrrol idine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- acetamido-2-hydroxy)butyl-3-(cis-propen-1-yl)-pyrrolidine-5- carboxylic acid t-butyl ester (yield : 0.0027 g, 100%).

1H NMR (DMSO-d6) 8 7.68 (d, J=9.6 Hz, 1H), 5.81 (m, 1H), 5.48 (m, 1H), 5.25 (m, 1H), 5.01 (dd, J=17.1, 1.8Hz, 1H), 4.95 (dd, J=10. 3,1.7Hz, 1H), 4.43 (t, J=8.5Hz, 1H), 4.10 (m, 1H), 3.74 (m, 1H), 3.56 (t, J=8.9Hz, 1H), 3.16 (quint., J=8.9Hz, 1H), 2.42 (dt, J=12. 8, 7.3Hz, 1H), 2.11 (M, 1H), 2.07 (m, 1H), 1.83 (s, 3H), 1.72 (dt, J=12. 8, 9.8Hz, 1H), 1.55 (dd, J=6.7,1.8Hz, 3H), 1.5-1.35 (m, 2H).

MS: (M+H) +=311, (M+Na) +=333, (M-H)-=309, (M+CF3COO-)-=423.

(2M-H)-=619.

Example 94 (~)-(2R,3S,5R,1'R,2'S,)-2-(1-Acetamido-2-methoxy-3-methyl)-3 -(cis- propen-1-yl)pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 94A (~)-(2R,3S,5R,1'R,2'S,)-1-t-butoxycarbonyl-2-(1-acetamido-2- methoxy- 3-methrl) pentyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic Acid t-Butyl Ester The title compound is prepared according to the method described in Example 84A, substituting ()- (2R, 3S, 5R, 1'R, 2'S, 3'S)-1-t-butoxycarbonyl-2-(1- acetamido-2-hydroxy-3-methyl) pentyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester in place of (i)-(2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- <BR> <BR> <BR> acetamido-2-hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester.

94B (~)-(2R,3S,5R,1'R,2'S,)-2-(1-Acetamido-2-methoxy-3-methyl)pe ntyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxvlic Acid Trifluoroacetic Acid Salt The title compound is prepared according to the method described in Example 41C, substituting ()- (2R, 3S, 5R, 1'R, 2'S, 3'S)-1-t-butoxycarbonyl-2-(1- acetamido-2-methoxy-3-methyl) pentyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- acetamido-2-hydroxy) butyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester.

Example 95 (~)-(2R,3S,5R,1'RS)-2-(1-Acetamido-2-oxo-2-heptafluoropropyl )ethyl-3-(cis- propen-1-vl)-pyrrolidine-5-carboxvlic Acid Trifluoroacetic Acid Salt 95A (~)-(2R,3S,5R,1'RS)-1-t-Butoxycarbonyl-2-(1-Acetamido-2-oxo- 3- heptafluoropropyl)-ethyl-3-(cis-propen-1-yl)-pyrroldine-5-ca rboxylic Acid t-Butyl Ester.

The title compound was prepared according to the method described in Example 42A, substituting ()- (2R, 3S, 5R, 1'R, 2'RS)-1-t-butoxycarbonyl-2- (1- <BR> <BR> <BR> <BR> acetamido-2-hydroxy-3-heptafluoropropyl) ethyl-3- (cis-propen-1-yl)-pyrrolidine-5- carboxylic acid t-butyl ester for ()- (2R, 3S, 5R, 1'R, 2'R)-1-t-butoxycarbonyl-2-(1- acetamido-2-hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 6.8 mg, 88%).

MS: (M+H) +=579, (M-H)-=577.

95B (~)-(2R,3S, 5R. 1'RS)-2- 1-Acetamido-2-oxo-2-heptafluoropropyl) ethyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41 C, substituting ()- (2R, 3S, 5R, 1'RS)2'S)-1-t-butoxycarbonyl-2-(1- acetamido-2-oxo-3-heptafluoropropyl) ethyl-3-(cis-propen-1-yl)-pyrrolidine-5- carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- 2- (1-acetamido-2-hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t- butyl ester (yield : 0.0037 g, 100%).

MS : (M+H) +=423, (M-H)-=421.

Example 96 ()- (2R. 3S. 5R. 1'RS)-2- (1-Acetamido-2-oxo-2-heptafluoropropyl) ethyl-3- (cis- propen-1-yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 96A (~)-(2R,3S, 5R. 1'RS)-1-t-Butoxvcarbonyl-2-(1-Acetamido-2-oxo-3- <BR> <BR> <BR> <BR> heptafluoroprop I) ethyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic Acid t-Butyl Ester.

The title compound was prepared according to the method described in Example 42A, substituting ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- acetamido-2-hydroxy-3-heptafluoropropyl) ethyl-3-(cis-propen-1-yl)-pyrrolidine-5- carboxylic acid t-butyl ester for ()- (2R, 3S, 5R, 1'R, 2'R)-1-t-butoxycarbonyl-2-(1- acetamido-2-hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 6.8 mg, 88%).

MS: (M+H)+=579, (M-H)-=577.

96B (2R. 3S. 5R, 1'RS)-2-(1-Acetamido-2-oxo-2-heptafluoropropyl)ethyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41C, substituting ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- acetamido-2-hydroxy-3-heptafluoropyl)ethyl-3-(cis-propen-1-y l)-pyrroldine-5- carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl- 2- (1-acetamido-2-hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t- butyl ester (yield : 0.0037 g, 100%).

MS: (M+H) +=423, (M-H)-=421.

Example 97 <BR> <BR> <BR> <BR> (s)-(2R. 3S. 5R. 1'R)-2-(1-Acetamido-2-oxo) pentyl-3-(cis-propen-1-yl)-nyrrolidine- 5-carboylic Acid Trifluoroacetic Acid Salt.

97A (~)-(2R,3S,5R,1'R,)-1-t-Butoxycarbonyl-2-(1-Acetamido-2-oxo- 3-(cis- propen-1-yl)-pyrrolidine-5-carboxylic Acid t-Butyl Ester.

The title compound was prepared according to the method described in Example 42A, substituting ()- (2R, 3S, 5R, 1'R, 2'R)-1-t-butoxycarbonyl-2-(1- acetamido-2-hdroxy)pentyl-3-(cis-propen-1-yl)-pyrroldine-5-c arboxylic acid t-butyl ester in place of (2R, 3S, 5R, 1'R, 2'S)-l-t-butoxycarbonyl-2- (l-acetamido-2- hydroxy) butyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 14 mg, 58%).

MS: (M+H) + = 453, (M+Na) + = 475 ; (M-H)-= 451.

97B (~)-(2R,3S,5R,1'R)-2-(1-Acetamido-2-oxo-3-(cis-propen-1-yl)- pyrrolidine-5-carboxvlic Acid Trifluoroacetic Acid Salt.

The title compound was prepared according to the method described in Example 41 C, substituting ()- (2R, 3S, 5R, 1'R)-1-t-butoxywarbonyl-2-(1- acetamido-2-oxo) pentyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2- (1-acetamido-2- hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 1.4 mg, 28%). rH NMR (DMSO-d6) # 8.31 (d, J=8.3Hz, 1 H), 5.40 (m, 1H), 5.19 (br t, 1H), 4.26 (t, J=6.8Hz, 1H), 3.63 (t, J=8.3Hz, 1 H), 3.35 (m, 1 H), 2.97 (m, 1H), 2.45 (m, 1 H), 2.34 (dt, J=3.4,7.4Hz, 1H), 2.20 (m, 1H), 1.84 (s, 3H), 1.58 (dd, J=2, 4.3Hz, 3H), 1.43 (m, 3H), 0.82 (t, J=7.3Hz, 3H) MS: (M-H)-= 295 ; (M+H) + = 297, (M+Na) + = 319.

Example 98 (~)-(2R,3S,5R,1'R)-2-(1-Acetamido-2-oxo-3-(cis-propen-1-yl)- pyrrolidine-5- carboxylic Acid Trifluoroacetic Acid Salt.

The title compound was prepared according to the method described in Example 41C, substituting ()- (2R, 3S, 5R, 1'R)-1-t butoxycarbonyl-2- (1- acetamido-2-oxo) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester prepared in Example 42A in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t- butoxycarbonyl-2- (1-acetamido-2-hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5- carboxylic acid t-butyl ester (yield : 5.0 mg, 100%).

1H NMR (DMSO-d6) 8 8.52 (d, J= 8.6Hz, 1 H), 5.47 (m, 1 H), 5.15 (m, 1H), 4.54 (m, 1H), 4.39 (dd, J= 11.0,6.7Hz, 1 H), 3.84 (t, J= 9. 2Hz, 1H), 3.17 (m, 1 H), 2.50 (m, 1 H), 2.38 (m, 1 H), 2.33 (m, 1 H), 1.83 (s, 3H), 1.63 (m, 1 H), 1.58 (dd, J= 6.7,1.8Hz, 3H), 0.94 (t, J= 7.5Hz, 3H).

MS : (M+H) += 283, (M+Na) + = 305, (M-H)-= 281.

Examples 99-115 The title compounds were prepared according to the methods described in Examples 20 and 40-42 by substituting the respective reactants.

Example 99 (~)-(2R,3S,5R,1'R)-2-(1-Acetamido-2-oxo-2-allyl)ethyl-3-(cis -propen-1-yl)- pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 1H NMR (DMSO-d6) 8 8.38 (d, J= 8.5Hz, 1H), 5.73 (m, 1H), 5.37 (m, 1H), 5.05 (m, 3H), 4.32 (t, J= 7.9Hz, 1H), 3. 90 (m, 1H), 3.49 (m, 1H), 3.13 (m, 2H), 2.98 (m, 1H), 3.18 (m, 1H), 1.78 (s, 3H), 1.51 (dd, J= 5.5,1.2Hz, 3H), 1.44 (m, 1H).

MS: (M+H)+=295. (M-H)-= 293.

Example 100 (~)-(2R,3S,5R,1'R,)-2-(1-Acetamido-2-oxo-3-methyl)butyl-3-vi nyl-pyrrolidine-5- carboxylic Acid Trifluoroacetic Acid Salt 1H NMR (DMSO-d6) # 8.64 (d, J= 8.5Hz, 1H), 5.59 (m, 1H), 5.08 (d, J= 17.1Hz, 1H), 5.02 (d, J= 9.8Hz, 1H), 4.65 (t, J= 8.6Hz, 1H), 4.32 (m, 1H), 3.82 (t, J= 9.2Hz, 1 H), 2.82 (m 2H), 2.36 (m, 1 H), 1.83 (s, 3H), 1.80 (m, 1 H), 1.03 (d, J= 6.7Hz, 3H), 0.97 (d, J= 6.7Hz, 3H).

MS: (M+H)+= 283, (M+Na) + = 305, (M-H)-= 281.

Example 101 (~)-(2R,3S,5R,1'R,)-2-(1-Acetamido-2-oxo-3)propyl-3-vinyl-py rrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 'H NMR (DMSO-d6) 8 8.96 (d, J= 7.9Hz, 1H), 5.71 (m, 1H), 5.27 (d, J=17. 7Hz, 1H), 5.17 (d, J= 11.0Hz, 1H), 4.38 (m, 1H), 4.29 (m, 1H), 3.81 (m, 1H), 2.61 (m, 1H), 2.22 (m, 1H), 2.13 (s, 3H), 2.01 (s, 3H), 1.24 (m, 1H).

MS: (M+H) += 255, (M+Na) + = 277, (M-H)-= 253.

Example 102 (~)-(2R,3S,5R,1'R,)-2-(1-Acetamido-2-oxo0butyl-3-vinyl-pyrro lidine-5- carboxylic Acid Trifluoroacetic Acid Salt 1H NMR (DMSO-d6) # 8.61 (d, J= 8.5Hz, 1H), 5.60 (m, 1H), 5.10 (d, J= 17.7Hz, 1H), 5.03 (dd, J= 10.4,1.2Hz, 1H), 4.54 (t, J= 8.5Hz, 1H), 4.38 (dd, J= 11.0,6. 7Hz, 1H), 3.86 (m, 1 H), 2.84 (m, 1 H), 2.52 (m, 1 H), 2.37 (m, 2H), 1. 85(s, 3H), 1.82 (m, 1 H), 0.94 (t, J= 7. 0Hz, 3H).

MS : (M=H)+=269, (M+Na)+=291, (M-H)-= 267.

Example 103 t)- (2R, 3S. 5R. 1'R)-2- (1-Acetamido-2-oxo) penyl-3-vinyl-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 'H NMR (DMSO-d6) # 8.60 (d, J= 9.7Hz, 1H), 5.60 (m, 1H), 5.07 (m, 2H), 4.65 (m, 1 H), 4.54 (m, 1 H), 4.38 (m, 1 H), 3.86 (m, 1 H), 2.84 (m, 1 H), 2.45 (m, 1 H), 2.36 (m, 1H), 1.86 (s, 3H), 1.82 (m, 1 H), 1.47 (m, 2H), 0.87 (t, J= 5.8Hz, 3H).

MS: (M+H)+= 283, (M+Na) += 305, (M-H)-= 281.

Example 104 (~)-2R, 3S. 5R, 1'R)-2-(1-Acetamido-2-hydroxv) ethyl-3-vinvl-pvrrolidine-5- carboxylic Acid Trifluoroacetic Acid Salt 1H NMR (DMSO-d6) 8 8.00 (d, J= 9.9Hz, 1H), 5.63 (m, 1H), 5.08 (m, 1H), 4.98 (m, 1 H), 4.35 (m, 1 H), 4.25 (m, 1 H), 4.08 (m, 1 H), 3.55 (m, 1 H), 3.45 (m, 1 H), 3.38 (m, 1 H), 2.83 (m, 1 H), 2.33 (m, 1 H), 1.78 (s, 3H).

MS: (M+H) += 243, (M+Na) += 265, (M-H)-= 241.

Example 105 2R.3S.5R.1'R.2'S)-2-1-Acetamido-2-hvdroxv)DroDv!-3-vinv!-Dvr ro))dine-5- carboxylic Acid Trifluoroacetic Acid Salt 1H NMR (DMSO-d6) # 7.96 (d, J= 9.7Hz, 1H), 5.74 (m, 1H), 5.12 (m, 1H), 5.03 (m, 1 H), 4.27 (m, 1 H), 3.96 (m, 1 H), 3.77 (m 1 H), 3.65 (m, 1 H), 2.87 (m, 1 H), 2.38 (m, 1 H), 1.82 (s, 3H), 1.80 (m, 1 H), 1.08 (d, J= 6. OHz, 3H).

MS: (M+H)+= 257, (M+Na) += 279, (M-H)-= 255.

Example 106 (~)-(2R,3S,5R, 1'R. 2'S)-2-(1-Acetamido-2-hydroxy)butyl-3-vinyl-pyrrolidine-5 carboxylic Acid Trifluoroacetic Acid Salt 'H NMR (DMSO-d6) 87. 99 (d, J= 9. OHz, 1H), 5.75 (m, 1H), 5.13 (d, J= 17. 1Hz, 1H), 5.04 (d, J= 10.5Hz, 1H), 4.27 (t, J= 8.4Hz, 1H), 4.04 (m, 1H), 3.78 (m, 1H), 3.48 (m, 1H), 2.89 (m, 1H), 2.40 (m, 1H), 1.88 (m, 1H), 1.85 (s, 3H), 1.54 (m, 1 H), 1.28 (m, 1H), 0.86 (t, J= 7.2Hz, 3H).

MS: (M+H) += 271, (M+Na) + = 293, (M-H)-= 269.

Example 107 (~)-(2R,3S,5R,1'R,2'S)-2-(1-Acetamido-2-hydroxy)pentyl-3-vin yl-pyrrolidine-5- carboxylic Acid Trifluoroacetic Acid Salt 'H NMR (DMSO-d6) 8 7.99 (d, J= 9.9Hz, 1H), 5.75 (m, 1H), 5.08 (m, 2H), 4.28 (m, 1 H), 4.03 (m, 1 H), 3.77 (m, 1 H), 3.52 (m, 1 H), 2.88 (m, 1 H), 2.40 (m, 1 H), 1.86 (s, 3H), 1.75 (m, 1 H), 1.45 (m, 2H), 1.25 (m, 2H), 0.87 (t, J= 5.9Hz, 3H).

MS: (M+H) += 285, (M+Na) += 307, (M-H)-=283.

Example 108 (~)-(2R,3S,5R,1'R,2'S)-2-(1-Acetamido-2-hydroxy-3-methyl)but yl-3-vinyl- pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 'H NMR (DMSO-d6) 8 7.97 (d, J= 9.3Hz, 1H), 5.75 (m, 1H), 5.12 (d, J= 17.1Hz, 1H), 5.04 (d, J= 11.2Hz, 1H), 4.24 (m, 1H), 4.13 (m, 1H), 3.74 (dd, J= 9.8,6. 1Hz, 1H), 3.44 (dd, J= 10.3,2. 0Hz, 1H), 2.87 (m, 1H), 2.40 (m, 1H), 1.84 (m, 1 H), 1.83 (s, 3H), 1.75 (m, 1 H), 0.89 (d, J= 6.8,3H), 0.75 (d, J= 6.8Hz, 3H).

MS : (M+H) += 285, (M+Na) + = 307, (M-H)-= 283.

Example 109 (~)-(2R,3S,5R,1'R,2S)-2-(1-Acetamido-2-hydroxy-2-cyclopropyl )ethyl-3-vinyl- pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 1H NMR (DMSO-d6) # 7.81 (d, J= 10.0Hz, 1H), 5.73 (m, 1H), 5.05 (m, 2H), 4.39 (m, 1H), 4.20 (m 1H), 3.90 (m, 1H), 3.61 (m, 1 H), 3.08 (m, 1H), 2.86 (m, 1H), 2.42 (m, 1H), 1.85 (s, 3H), 0.88 (m, 1H), 0.45 (m, 1H), 0.35 (m, 2H), 0.11 (m, 1H).

MS: (M+H)+= 283, (M+Na) += 305, (M-H)-= 281.

Example 110 (~)-(2R,3S,5R, 1'R. 2'R)-2-91-Acetamido-2-hydroxy)propyl-3-vinyl-pyrrolidine-5- carboxylic Acid Trifluoroacetic Acid Salt 1H NMR (DMSO-d6) 6 7.77 (d, J= 9.7Hz, 1H), 5.72 (m, 1H), 5.07 (m, 2H), 4.40 (m, 1 H), 4.03 (m, 1 H), 3.95 (m 1 H), 3.57 (m, 1 H), 2.86 (m, 1 H), 2.43 (m, 1 H), 1.88 (m, 1 H), 1.84 (s, 3H), 1.04 (d, J= 6. 0Hz, 3H).

MS: (M+H) += 257, (M+Na) += 279, (M-H)-= 255.

Example 111 (~)-(2R,3S,5R, 1'R. 2'R)-2- (1-Acetamido-2-hydroxy) buyl-3-vinyl-pyrrolidine-5- carboxylic Acid Trifluoroacetic Acid Salt 'H NMR (DMSO-d6) 87. 72 (d, J= 9.8Hz, 1H), 5.73 (m, 1H), 5.08 (d, J= 17.1Hz, 1H), 5.03 (d, J= 10.4Hz, 1H), 4.41 (m, 1H), 4.13 (m, 1H), 3.68 (m, 1H), 3.63 (m, 1 H), 2.88 (m, 1 H), 2.44 (m, 1 H), 1.90 (m, 1 H), 1.83 (s, 3H), 1.38 (m, 2H), 0.84 (t, J= 7.3Hz, 3H).

MS: (M+H) += 271, (M+Na) + = 293, (M-H)-= 269.

Example 112 (~)-(2R,3S, 5R. 1'R. 2R)-2-(1-Acetamido-2-hydroxy)pentyl-3-vinyl-pyrrolidinet-5- carboxylic Acid Trifluoroacetic Acid Salt 'H NMR (DMSO-d6) 8 7.72 (d, J= 9. 9Hz, 1H), 5.72 (m, 1H), 5.06 (m, 2H), 4.42 (m, 1 H), 4.09 (m, 1 H), 3.77 (m, 1 H), 3.61 (m, 1H), 2.87 (m, 1H), 2.43 (m, 1 H), 1.90 (m, 1 H), 1.83 (s, 3H), 1.37 (m, 2H), 1.27 (m, 2H), 0.87 (t, J= 5.9Hz, 3H).

MS: (M+H)+=285. (M+Na) += 307, (M-H)-= 283.

Example 113 (~)-(2R,3S,5R,1'R,)-2-(1-Acetamido-2-hydroxymethyl)-butyl-3- vinyl- pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 'H NMR (DMSO-d6) # 7.71 (d, J= 9.3Hz, 1H), 5.70 (m, 1H), 5.08 (d, J= 17. 1Hz, 1H), 5.03 (d, J= 10.3Hz, 1H), 4.42 (m, 1H), 4.25 (m, 1H), 3.61 (m, 1H), 3.35 (dd, J= 8.3,2.5Hz, 1H), 2.90 (m, 1H), 2.44 (m, 1H), 1.92 (m, 1H), 1.82 (s, 3H), 1.58 (m, 1H), 0.95 (d, J= 6.8Hz, 3H), 0.79 (d, J= 6.4Hz, 3H).

MS: (M+H)+=285, (M+Na) + = 307, (M-H)-= 283.

Example 114 (~)-(2R,3S,5R,1'R,2'R)-2-(1-Acetamido-2-cyclopropyl)ethyl-3- vinyl- pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 'H NMR (DMSO-d6) 8 7.94 (d, J= 9.6Hz, 1H), 5.76 (m, 1H), 5.12 (m, 2H), 4.40 (m, 1H), 4.21 (m, 1H), 3.90 (m, 1H), 3.53 (m, 1H), 3.13 (m, 1H), 2.81 (m, 1H), 2.25 (m, 1H), 1.87 (s, 3H), 0. 90 (m, 1H), 0.47 (m, 1H), 0.37 (m, 2H), 0.15 (m, 1H).

MS: (M+H)+=283, (M+Na)+= 305, (M-H)-=281.

Example 115 (~)-(2R,3S,5R,1'R,2'R)-2-(1-Acetamido-2-hydroxy-4-methyl)pen tyl-3-vinyl- pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 'H NMR (DMSO-d6) 8 7.71 (d, J= 9.7Hz, 1H), 5.83 (m, 1H), 5.06 (d, J= 17.1Hz, 1H), 5.02 (d, J= 10.3Hz, 1H), 4.41 (m, 1H), 4.06 (m, 1H), 3.83 (m, 1H), 3.59 (t, J= 8.8Hz, 1 H), 2.84 (m, 1 H), 2.42 (m, 1 H), 1.90 (m, 1 H), 1.82 (s, 3H), 1.71 (m, 1 H), 1.34 (m, 1 H), 1.07 (m, 1 H), 0.89 (d, J= 6. 8Hz, 3H), 0.86 (d, J= 6.3Hz, 3H).

MS: (M+H)+= 299, (M+Na) + = 321, (M-H)-= 297.

Example 116 (~)-(2R,3S,5R,1'R)-2-(1-Acetamido-2-hydroxy-2-methyl)pentyl- 3-vinyl-pyrrolidine- 5-carboxylic Acid Trifluoroacetic Acid Salt 116A t- (2R, 3S, 5R. 1'R)-t-Butoxvcarbonyl-2- (1-Acetamido-2-hydroxv-2- methyl) propyl-3-vinyl-pyrrolidine-5-carboxylic Acid t-Butyl Ester.

()- (2R, 3S, 5R, 1'R)-t-Butoxycarbonyl 2- (1-acetamido-2-oxo) propyl-3-vinyl- pyrrolidine-5-carboxylic acid t-butyl ester (11 mg, 0.027 mmol) was reacted with methyl magnesium bromide (3 M) (0.05mL, 0.134 mmol) in THF (2 mL) at 25 °C for 2 hours. The reaction was quenched with saturated aqueous ammonium chloride (2 mL) and water (2 mL) followed by extraction using dichloromethane (2 X 5 mL). The organic layer was dried over magnesium sulfate, filtered and concentrated in vacuo. The residue was purified by column chromatography on silica gel using 2/1: ethyl acetate/hexane to provide the title compound (yield : 1.9 mg, 17%).

MS : (M+H) +=427, (M-H)'=425 116B (~)-(2R,3S,5R,1'R)-2-(1-Acetamido-2-hydroxy-2-methyl)pentyl- 3-vinyl- pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41C, substituting ()- (2R, 3S, 5R, 1'R)-1-t-butoxycarbonyl-2-(1- <BR> <BR> <BR> acetamido-2-hydroxy-2-methyl) propyl-3-vinyl-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxywarbonyl-2-(1-acetamido-2- hydroxhy)butyl-3-(cis-propen-1-yl)-pyrroldine-5-caboxylic acid t-butyl ester (yield : 1.6 mg, 99%).

'H NMR (DMSO-d6) 8 7.70 (d, J=9.9Hz, 1H), 5.75 (m, 1H), 5.02 (m, 2H), 4.37 (m, 1 H), 4.15 (m, 1 H), 3.61 (m, 1 H), 2.78 (m, 1 H), 2.41 (m, 1 H), 1.81 (s, 3H), 1.20 (s, 3H), 1.12 (s, 3H) MS: (M+H)+=271, (M+23)+=293, (M-H)-=269 Example 117 (i)-(2R, 3S, 5R. 1'R)-2-L-Acetamido-2-hvdroxy-2-ethyl) butyl-3-vinvyrrolidine-5- carboxylic Acid Trifluoroacetic Acid Salt 117A )- (2R. 3S. 5R. 1'R)-t-Butoxycarbonvl-2- 1-Acetamido-2-hydroxy-2- ethyl) l)-butyl-3-vinyl-pyrroldine-5-carboxylic Acid t-Butyl Ester.

()- (2R, 3S, 5R, 1'R)-t-Butoxycarbonyl-2- (1-acetamido-2-oxo) butyl-3-vinyl- pyrrolidine-5-carboxylic acid t-butyl ester (37mg, 0.087 mmol) was reacted with ethyl magnesium bromide (3 M) (0.15mL, 0.44mmol) in THF (5 mL) at 25 °C for 2 hours. The reaction was quenched with saturated aqueous ammonium chloride 5 mL) and water (5 mL) followed by extraction using dichloromethane (2 X 10 mL). The organic layer was dried over magnesium sulfate, filtered and concentrated in vacuo. The residue was purified by column chromatography on silica gel using 2/1 : ethyl acetate/hexane to provide the title compound (yield : 14 mg, 35%).

MS : (M+H) += 455, (M-H)-=453 116B %(~)-(2R,3S,5R,1'R)-2-(1-Acetamido-2-hydroxy-2ethyl)butyl-3- vinyl- nvrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41C, substituting ()- (2R, 3S, 5R, 1'R)-1-t-butoxywarbonyl-2-(1- acetamido-2-hydroxy-2-ethyl) butyl-3vinyl-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2- (1-acetamido-2- hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 5.8 mg, 98%).

'H NMR (DMSO-d6) # 7.62 (d, J=9.6HZ, 1H), 5.75 (m, 1 H), 5.03 (m, 2H), 4.39 (m, 1 H), 4.31 (m, 2H), 3.87 (m, 1 H), 3.38 (m, 1 H), 2.88 ( m, 1 H), 2.40 (m, 1 H), 1.83 (s, 3H), 1.55-1.30 (m, 4H), 0.86 (m, 6H) MS: (M+H) + =299, (M-H)-=297 Example 118 (~)-(2R, 3S. 5R. 1'S)-2- 1-Acetamido) allyl-3- (cis-propen-1-yl)-pyrrolidine-5- carboxylic Acid Trifluoroacetic Acid Salt 118A (~)-(2R,3S,5R,1'S)-2-(1-Acetamido-2-(1-acetamido)allyl-3-(ci s-propen- 1-vl)-pyrrolidine-5-carboxylic Acid t-Butyl Ester The title compound was prepared according to the method described in Example 20K, substituting ()- (2R, 3S, 5R, 1'S)-1-t-butoxycarbonyl-2-(1-acetamido- 2-formyl) methyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'S)-1-t-butoxycarbonyl-2- (1-acetamido-3-methyl) butyl-3- formyl-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 15.3 mg, 61.4%).

MS: (M+H) += 409. 118B (~)-(2R,3S,5R,1'S)-2-(1-Acetamido)allyl-3-(cis-propen-1-yl)- pyrrolidine-5- carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41C, substituting %(~)-(2R,3S,5R,1'S)-1-t-butyoxycarbonyl-2-(1- acetamido) allyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1-acetamido-2- hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 13.1 mg, 100%).

1H NMR (DMSO-d6) : 81. 58 (dd, 3H), 1.74 (dt, 1H), 1.88 (s, 3H), 2.41 (dt, 1H), 3.17 (m, 1H), 3.56 (dd, 1H), 4.35 (dd, 1H), 4.70 (dd, 1H), 5.22-5.30 (m, 3H), 5.51 (m, 1H), 5.82 (m, 1H), 8.15 (d, 1H), 9.18 (br s, 2H).

MS: (M+H) += 253.

Example 119 (~)-(2R,3S,5R,1'S)-2-(1-Acetamido-2-(cis and trans) buten-1-yl)-3-(cis-propen-1- vl)-pyrrolidine-5-carboxvlic Acid Trifluoroacetic Acid Salt 119A (i)-(2R. 3S. 5R. 1'S)-1-t-Butoxycarbonyl-2-(1-Acetamido-2-(cis and trans)buten-1-yl)-3-(cis-propen-1-yl)-pyrrolidine-5-carboxyl ic Acid t-Butyl Ester The title compound was prepared according to the method described in Example 20K, substituting ()- (2R, 3S, 5R, 1'S)-1-t-butoxycarbonyl-2-(1-acetamido- 2-formyl) methyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'S)-1-t-butoxycarbonyl-2-(1-acetamido-3-methyl)butyl-3- formyl-pyrrolidine-5-carboxylic acid t-butyl ester and ethyltriphenylphosphonium bromide for methyltriphenylphosphonium bromide (yield : 12.4 mg, 48.2%).

MS: (M+H) += 423 119B (~)-(2R,3S,5R,1'S)-2-(1-Acetamido-2-(cis and trans) buten-1-yl)-3-(cis- propen-1-yl)-pyrroldine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41 C, substituting ()- (2R, 3S, 5R, 1'S)-1-t-butoxycarbonyl-2-(1-acetamido- 2-(cis and trans) buten-1-yl)-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t- butyl ester in place of (~)-(2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1-acetamido- 2-hydroxy) butyl-3-(cis-propen-1-yl)-pyrroldine-5-carboxylic acid t-butyl ester (yield : 11.8 mg, 100%).

1H NMR (DMSO-d6) : 81. 63 (dd, 3H), 1.66 (dd, 3H), 1.74 (m, 1H), 1.88 (s, 3H), 2.41 (dt, 1H), 3.17 (m, 1 H), 3.50 (dd, 1H), 4.34 (dd, 1H), 4.95 (m, 1H), 5.23 (m, 1H), 5.39 (m, 1H), 5.53 (m, 1H), 5.68 (m, 1H), 8.21 (d, 1H), 9.18 (br s, 2H).

MS: (M+H)+= 267 Example 120 (i)-(2R. 3S. 5R. 1'S)-2-(1-Acetamido-3. 3-dimethvl) allyl-3-(cis-propen-1-vl)- pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 120A (t)- (2R, 3S. 5R, 1'S)-1-t-Butoxvcarbonyl-2- 1-acetamido-3. 3-dimethvl) allyl-3= (cis-propen-1-yl)-pyrrolidine-5-carboxylic Acid t-Buyl Ester The title compound was prepared according to the method described in Example 20K, substituting ()- (2R, 3S, 5R, 1'S)-1-t-butoxycarbonyl-2- (1-acetamido- 2-formyl) methyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3R, 5R, 1'S)-1-t-butoxycarbonyl-2- (1-acetamido-3-methyl) butyl-3- formyl-pyrrolidine-5-carboxylic acid t-butyl ester and isopropyltriphenylphosphonium bromide for methyltriphenylphosphonium bromide (yield : 8.2 mg, 25.9%).

MS: (M+H) += 437 120B (~)-(2R,3S,5R,1'S)-2-(1-Acetamido-3,3-dimethyl)allyl-3-(cis- propen-1-yl)- pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41C, substituting ()- (2R, 3S, 5R, 1'S)-1-t-butoxycarbonyl-2-(1-acetamido- 3,3-dimethyl) allyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester in place of (t)- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2- (1-acetamido-2- hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester. ester (yield : 7.5 mg, 100%).

'H NMR (DMSO-d6) : 81. 53 (dd, 3H), 1.57 (s, 3H), 1.61 (s, 3H), 1.66 (m, 1 H), 1.77 (s, 3H), 2.32 (dt, 1 H), 3.07 (m, 1 H), 3.39 (dd, 1 H), 4.26 (m, 1 H), 4.75 (m, 1H), 5.07 (d, 1H), 5.15 (m, 1H), 5.44 (m, 1H), 8.06 (d, 1H).

MS: (M+H) += 281.

Example 121 (~)-(2R,3S,5R,1'S)-2-(1-Acetamido-2-(cis and trans)penten-1-yl)-3-(cis-propen-1- vl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 121A (i)-(2R. 3S. 5R. 1'S)-1-t-Butoxycarbonyl-2-(1-Acetamido-2-(cis and<BR> <BR> <BR> <BR> <BR> <BR> <BR> trans) penten-1-yl)-3- (cis-propen-1-pyrrolidine-5-carboxylic Acid t-Butyl Ester The title compound was prepared according to the method described in Example substituting ()- (2R, 3S, 5R, 1'S)-1-t-butoxycarbonyl-2-(1-acetamido-2- formyl) methyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3R, 5R, 1'S)-1-t-butoxycarbonyl-2- (1-acetamido-3-methyl) butyl-3- formyl-pyrrolidine-5-carboxylic acid t-butyl ester and n-butyl- triphenylphosphonium bromide for methyltriphenylphosphonium bromide (yield : 21.0 mg, 66.2%).

MS: (M+H) += 437.

121B (~)-(2R,3S,5R,1'S)-2-(1-Acetamido-2-(cis and trans) penten-1-yl)-3-(cis- propen-1-vl)-pvrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41C, substituting ()- (2R, 3S, 5R, 1'S)-1-t-butoxycarbonyl-2-(1-acetamido- 2-(cis and trans) penten-1-yl)-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t- butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2- (1-acetamido- 2-jydroxy)butyl-3-9cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester. ester (yield: 16.0 mg, 98.1%).

1H NMR (DMSO-d6) : # 0.93 (t, 3H), 1.62 (dd, 3H), 1.75 (m, 1H), 1.87 (s, 3H), 2.07 (m, 2H). 2.40 (m, 1H), 3.17 (m, 1H), 3.50 (m, 1 H), 4.34 (m, 1H), 4.94 (m, 1H), 5.23 (m, 1H), 5.34 (m, 1H), 5.53 (m, 1H), 5.58 (m, 1H), 8.24 (d, 1H), 9.25 (br s, 2H).

MS: (M+H) +=281.

Example 122 (~)-(2R, 3S. 5R. 1'S)-2-(1Acetamido-4-hydroxy-2-(cis-and trans)buten-1-yl)-3-(cis- propen-1-yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 122A (~)-(2R,3S,5R,1'S)-1-t-Butoxycarbonyl-2-(1-Acetamido-4-(t- <BR> <BR> <BR> butyidimethylsilyloxy)-2- (cis and trans) buten-1-yl)-3- (cis-propen-1-yl)-pyrrolidine-<BR> <BR> <BR> 5-carboxylic Acid t-Butyl Ester The title compound was prepared according to the method described in Example substituting ()- (2R, 3S, 5R, 1'S)-1-t-butoxycarbonyl-2- (1-acetamido-2- formyl) methyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3R, 5R, 1'S)-1-t-butoxycarbonyl-2- (1-acetamido-3-methyl) butyl-3- formyl-pyrrolidine-5-carboxylic acid t-butyl ester and 4- (t-butyidimethylsilyloxy)- butyltriphenylphosphonium bromide for methyltriphenylphosphonium bromide (yield : 23. 1 mg, 66.9%).

MS : (M+H)'= 567.

122B(~)-(2R,3S,5R,1'S)-2-(1-Acetamido-4-hydroxy-2-(cis and trans)buten-1-yl)- 3- (cis-propen-1-vl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41 C, substituting ()- (2R, 3S, 5R, 1'S)-1-t-butoxycarbonyl-2-(1-acetamido- 2- (cis and trans) (cis-propen-1-yl)-pyrrolidine-5- carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl- 2-(1acetamido-2-hydroxy)butyl-3-(cis-propen-1-yl)-pyrroldine -5-carboxylic acid t- butyl ester (yield : 16.9 mg, >100%).

H NMR (DMSO-d6) : 81. 67 (dd, 3H), 1.78 (dt, 1H), 1.91 (s, 3H), 2.44 (m, 1 H), 2.50 (m, 1 H), 2.56 (m, 1 H), 2.65 (m, 1 H), 3.23 (m, 1 H), 3.54 (m, 1 H), 4.40 (m, 1 H), 4.47 (m, 2H), 5.01 (m, 1H), 5.26 (m, 1H), 5.54 (m, 2H), 5.63 (m, 1H), 8.32 (d, 1H), 9.27 (br s, 2H).

MS: (M+H)+= 297.

Example 123 (~)-(2R,3S,5R,1'S)-2-(1-Acetamido)-butyl-3-vinyl-pyrrolidine -5-carboxylic Acid Hydrochloride 123A (~)-(2R,3S,5R)-1-Benzyl-2-vinyl-3-t-butyldiphenylilyloxymeth yl- pyrrolidine-5-carboxylic Acid t-Butyl Ester.

()- (2R, 3R, 5R)-1-Benyzyl-2-vinyl-3-hydroxymethyl-pyrroldine-5carboxylic acid t-butyl ester (30.8 g, 97.1 mmol) was reacted with t-butyidiphenylsilyl chloride (49.5 mL, 190.4 mmol) and imidazole in dichloromethane (650 mL) at 0 °C for 1 hour. The reaction was quenched methanol followed by extraction with dichloromethane (600 mL). The organic layer was dried over magnesium sulfate, filtered and concentrated in vacuo. The residue was purified by column chromatography on silica gel using 2/1: chloroform/hexane to provide the title compound (yield : 52.9 g, 98%).

1H NMR (CDCl3) 7.62-7.67 (m, 4H), 7.32-7.44 (m, 6H), 7.25-7.30 (m, 5H), 5.58-5.72 (m, 1H), 5.06-5.14 (m, 2H), 3.90 (d, 1 H), 3.72-3.78 (m, 1H), 3.58-3.68 (m, 2H), 3.44-3.52 (m, 2H), 2.26-2.40 (m, 1 H), 2.10-2.23 (m, 1H), 1.68-1.78 (m, 1H), 1.38 (s, 9H), 1.03 (s, 9H).

MS: (M+H) +=556 123B(t)- (2R, 3R. 5R. 1'RS)-1-Benzyy (1. 2-dihydroxy) ethyl-3-t-<BR> <BR> <BR> butdiphenylsilyloxymethyl-pyrrolidine-5-carboxylic Acid t-Butyl Ester.

()- (2R, 3R, 5R)-1-Benzyl-2-vinyl-3-t-butyldiphenylsilyloxymethyl- pyrrolidine-5-carboxylic acid t-butyl ester (22.7 g, 41 mmol) was reacted with Os04 (4%) (2.5 mL, 0.7 mol. %) and N-methyl morpholine N-oxide (18.5 g, 2.77 eq.) in acetone (500 mL) and water (60 mL) for 48h at room temperature. The reaction was quenched with 10% aqueous Na2S203 (200 mL). The reaction was concentrated in vacuo and the residue was partitioned between ethyl acetate/water. The organic layer was dried over magnesium sulfate, filtered and concentrated in vacuo. The residue was purified by column chromatography on silica gel using 35% ethyl acetate/hexane to provide the title compound (yield : 11 g, 55%).

1H NMR (DMSO-d6) 8 7.58-7.63 (m, 5H), 7.40-7.48 (m, 7H), 7.20-7.35 (m, 3H), 4.41-4.45 (m, 2H), 3.98 (d, 1 H), 3.75-3.84 (m, 2H), 3.50-3.68 (m, 2H), 3.4- 3.46 (m, 1 H), 3.16-3.25 (m, 1H), 2.97-3.0 (m, 1H), 2.09-2.28 (m, 1H), 1.62-1.89 (m, 1H), 1.34-1.39 (m, 1H), 1.30 (s, 9H),. 98,. 96 (2s, 9H).

MS: (M+H) +=590 123C (~)-(2R,3S,5R,1'RS)-2-(1,2-dihydroxy)ethyl-3-t- butyidiphenylsilvioxymethyl-nvrrolidine-5-carboxylic Acid t-Butyl Ester.

(~)-(2R, 3R, 5R, 1'RS)-1-Benzyl-2- (1, 2-dihydroxy) ethyl-3-t- butyidiphenylsilyloxymethyl-pyrrolidine-5-carboxylic acid t-butyl ester (11 g, 18.7 mmol) was reacted under 1 atmosphere of hydrogen with 20% Pd (OH) 2/C (5 g) and in ethanol (40 mL) vigorously stirred for 2.5 days at room temperature. The reaction was filtered, and the catalyst was washed with methanol (3x30 mL). The filtrate was evaporated in vacuo to give the title compound as an oil (yield : 8 g, 94%) 123D(t)- (2R. 3R. 5R, 1'R)-1-f-Butoxycarbonyl-2- (1. 2-dihydroxy) ethyl-3-t- butyldiphenylsilyloxymethyl-pyrroldine-5-carboxylic Acid t-Butyl Ester.

The title compound was prepared according to the method described in Example 40D, substituting ()- (2R, 3R, 5R, 1'RS)-2- (1, 2-dihydroxy) ethyl-3-t- butyidiphenylsilyloxymethyl-pyrrolidine-5-carboxylic acid t-butyl ester in place of (2R, 3R, 5R, 1'RS)-2- (1, 2-dihydroxy) ethyl-3-acetoxymethyl-pyrrolidine-5-carboxylic acid t-butyl ester. The residue was purified by column chromatography on silica gel using 35% ethyl acetate/hexane to provide the title compound (yield : 20.5 g, 60%).

'H NMR (DMSO-d6) 7.57-7.60 (m, 4H), 7.38-7.48 (m, 6H), 4.85,4.77 (2d, 1H), 4.45-4.50 (m, 1 H), 4.02-4.10 (m, 1 H), 3.80-3.95 (m, 1 H), 3.73,3.68 (2s, 1 H), 3.45-3.67 (m, 2H), 3.18-3.28 (m, 2H), 2.36-2.46 (m, 2H), 1.86,1.70 (2d, 1H), 1.40,1.35 (2s, 9H), 1.32,1.26 (2s, 9H), 1.0,0.98 (2s, 9H).

MS: (M+H)'= 600 123 E (i)-(2R, 3R, 5 R, 1'R)-1-t-Butoxycarbonyl-2-(1-methanesulfonyloxy-2-<BR> <BR> <BR> <BR> <BR> <BR> acetoxv)ethyl-3-t-butyidiphenylsilymethyl-pvrrolidine-5-carb oxylic Acid t-Butyl Ester.

()- (2R, 3R, 5R, 1'R)-1-t-Butoxycarbonyl-2- (1, 2-dihydroxy) ethyl-3-t- butyldiphenylsilyloxymethyl-pyrrolidine-5-carboxylic acid t-butyl ester (20.5 g, 34.2 mmole) was reacted with acetic anhydride (16.1 mL, 171 mmole) and triethylamine (47.7 mL, 342 mmole) in dichloromethane (360 mL) at 0°C for 16h.

The reaction was treated with methanol (35 mL) for 10 minutes and diluted with dichloromethane (1300 mL). The organic layer was washed with water, and brine, dried over MgS04, filtered and concentrated in vacuo. The residue was reacted with methanesulfonyl chloride (4.0 mL, 51.3 mmole) and triethylamine (14.3 mL, 103 mmole) in dichloromethane (350 mL) at 0°C for 1.5 hours. The reaction was quenched with water (300 mL) and diluted with dichloromethane (1200 mL). The organic layer was washed with water, and brine, dried over MgS04, filtered and concentrated in vacuo. The residue was purified by chromatography on silica gel using 30% ethyl acetate/hexanes to provide the title compound (yield : 23.8 g, 97%).

'H NMR (DMSO-d6) 87. 58-7.62 (m, 4H), 7.38-7.50 (m, 6H), 5.12-5.26 (2m, 1H), 4.06-4.25 (m, 3H), 4.00 (d, 1 H), 3.46-3.68 (m, 2H), 3.20,3.18 (2s, 3H), 2.40- 2.48 (m, 1H), 2.02,1.99, (2s, 3H), 1.68-1.88 (m, 1H), 1.42,1.36 (2s, 9H), 1.31,1.25 (2s, 9H), 1.00,0.98 (2s, 9H).

MS: (M+H) += 720, (M+NH4) +=737 123F (t)- (2R. 3R. 5R. 1'S)-1-t-Butoxvcarbonyl-2-oxiranyl-3-t- butvidiphenylsilyloxymethyl-pyrrolidine-5-carboxylic Acid t-Butyl Ester.

()- (2R, 3R, 5R, 1'S)-1-t-Butoxywarbonyl-2-(1-methanesulfonyloxy-2- acetoxy) ethyl-3-t-butyidiphenylsilyloxymethyl-pyrrolidine-5-carboxyl ic acid t-butyl ester (23.8 g, 33.1 mmole) was reacted with potassium carbonate (10.1 g, 66.2 mmole) in methanol (160 mL) and THF (160 mL) at 25 °C for 18 hours. The reaction was concentrated in vacuo. The residue was dissolved in ethyl acetate and washed with water, and brine, dried over MgS04, filtered and concentrated in vacuo. The residue was purified by chromatography on silica gel using 25% ethyl acetate/hexanes to provide the title compound, as an oil (yield : 16.7 g, 87%).

'H NMR (CDC13) 6 7.60-7.68 (m, 4H), 7.32-7.45 (m, 6H), 4.02-4.28 (m, 2H), 3.67-3.78 (m, 1 H), 3.52-3.62 (m, 1 H), 3.0-3. 08 (m, 1 H), 2.68-2.75 (m, 1 H), 2.47-2.52 (m, 3H), 1.80-1.90 (m, 1 H), 1.48,1.42 (2s, 9H), 1.37,1.35 (2s, 9H), 1.07,1.03 (2s, 9H).

MS: (M+H) += 582 123G (i)-(2R, 3R. 5R. 1'S)-1-t-Butoxycarbonyl-2-oxiranyl-3-hvdroxvmethyl- pyrrolidine-5-carboxylic Acid t-Butyl Ester.

()- (2R, 3R, 5R, 1'S)-1-t-butoxycarbonyl-2-oxiranyl-3-t- butyldiphenylsilyloxymethyl-pyrrolidine-5-carboxylic acid t-butyl ester (4.17 g, 7.2 mmole) was reacted with tetrabutylammonium fluoride (1 M) (14 mL, 14.0 mmole) in THF (7 mL) for 20 minutes at 0°C then for 1.5 hours at 25°C. The reaction was concentrated in vacuo the residue was dissolved in ethyl acetate and washed with pH 7.0 buffer and brine, dried over MgS04, filtered and concentrated in vacuo. The residue was purified by chromatography on silica gel using 50% ethyl acetate/hexanes to provide the title compound, as an oil (yield : 2.4 g, 97%).

1H NMR (DMSO-d6) 8 4.72-4.78 (m, 1H), 3.94-4.05 (m, 2H), 3.35-3.47 (m, 1H), 3.18-3.28 (m, 1H), 3.03-3.08 (m, 1H), 2.63-2.73 (m, 1H), 2.37-2.44 (m, 1H), 2.30-2.36 (m, 1H), 2.08-2.20 (m, 1H), 1.58-1.75 (m, 1H), 1.40 (s, 9H), 1.37,1.34 (2s, 9H).

MS: (M+H) += 344, (M+Na)+= 366 123H ()- (2R, 3R. 5R. 1'S)-1-t-Butoxycarbon, rl-2-oxiranyl-3-formyl-pyrrolidine-5- carboxylic Acid t-Butyl Ester.

()- (2R, 3R, 5R, 2'S)-1-t-butoxycarbonyl-2-oxiranyl-3-hydroxymethyl-1 pyrrolidine-5-carboxylic acid t-butyl ester (2.4 g, 7.0 mmole) and triethylamine (3.9 mL 28.0 mmole) in dichloromethane (70 mL) at 0°C was reacted with sulfur trioxide pyridine complex (3.35 g, 21.0 mmole) in dimethylsulfoxide (21 mL) by dropwise addition followed by reaction for an additional 3 hours. The reaction was quenched with water (50 mL) and diluted with ethyl acetate (200 mL). The organic layer was washed with water, and brine, dried over MgS04, filtered and concentrated in vacuo to provide the title compound (yield : 2.2 g,).

1H NMR (DMSO-d6) (rotamers) 8 9.58 and 9.56 (2s, 1H), 4.70 and 4.53 (2m, 1 H), 3.96 (dd, J=1.4,9.2 Hz, 1 H), 3.25-3.20 (m, 1 H), 2. 91 (m, 1 H), 2.71 (m, 1H), 2.50-2.28 (m, 3H), 1.42,1.37,1.34, and 1.30 (4s, 18H) MS: (M-H)-= 340 1231 (~)-(2R,3S,5R,1'S)-1-t-Butoxycarbonyl-2-oxiranyl-3-vinyl-pyr rolidine-5- carboxylic Acid t-Butyl Ester.

Triphenylphosphoranylidenemethyl ylide (17.6 mmole) prepared by reacting methyltriphenylphosphonium bromide (12.63 g, 35.4 mmole) and potassium tert-butoxide (1 M). (17. 6 mL, 17.6 mmole) in THF (70 mL) for 1 hour at 25°C. ()- (2R, 3R, 5R, 1'S)-1-t-Butoxycarbonyl-2-oxiranyl-3-formyl-pyrrolidine-5- carboxylic acid t-butyl ester (2.2 g, 6.5 mmole) in THF (10 mL) was added to the above solution at 0°C and stirred for 0.5 hours. The reaction was quenched with saturated ammonium chloride (50 mL) and diluted with ethyl acetate (200 mL).

The organic layer was washed with water, and brine, dried over MgS04, filtered and concentrated in vacuo. The residue was purified by chromatography on silica gel using 10% ethyl acetate/hexanes to provide the title compound (yield : 2 g, 84%).

'H NMR (DMSO-d6) 8 5.80-5.95 (m, 1H), 5.08 (d, 1H), 4.94-5.04 (1 H), 4.00-4.07 (m, 1H), 3.59,3.90 (2t, 1H), 3.07-3.16 (m, 1H), 2.73-2.81 (m, 1H), 2.65- 2.72 (m, 1H), 2.35-2.48 (m, 1H), 1.59-1.76 (m, 1H), 1.42 (s, 9H), 1.38,1.35 (2s, 9H).

MS: (M+H)+= 340 123J ()- (2R. 3S. 5R, 1'R)-1-t-Butoxycarbonyl-2- (1-methanesulfonyloxy-3- azido) ethvl-3-vinil-pvrrolidine-5-carboxylic Acid t-Butyl Ester.

(t)-(2R, 3S, 5R, 2'S)-1-t-Butoxycarbonyl-2-oxiranyl-3-vinyl-pyrrolidine-5- carboxylic acid t-butyl ester (1.72 g, 5.1 mmole) and ammonium chloride (1.36 g,.

25.4 mmole) in ethanol (45 mL) and water (5 mL) was reacted with lithium azide (1.2 g, 24.5 mmole) for 7 hours at 50°C. The reaction was concentrated in vacuo and diluted with ethyl acetate (200 mL). The organic layer was washed with water, and brine, dried over MgS04, filtered and concentrated in vacuo,. The residue (2.15 g) was dissolved in dichloromethane (50 mL) and reacted with methanesulfonyl chloride (0.8 mL, 10.2 mmole) and triethylamine (2.8 mL, 20.4 mmole) for 0.5 hours at 0°C. The reaction was quenched with aqueous sodium bicarbonate (50 mL) and diluted with ethyl acetate (200 mL). The organic layer was washed with water, and brine, dried over MgS04, filtered and concentrated in vacuo. The residue was purified by chromatography on silica gel using 10% ethyl acetate/hexanes to provide the title compound (yield : 1.87 g, 80%).

'H NMR (DMSO-d6) 8 5.77-5.98 (m, 1H), 4.94-5.11 (m, 3H), 4.12-4.19 (m, 1H), 3.99-4.06 (m, 1H), 3.66,3.71 (2d, 1H), 3.25,3.22 (2s, 3H), 2.92-3.02 (m, 1H), 2.55-2.63 (m, 1H), 1.68-1.82 (m. 1H), 1.45,1.42 (2s, 9H), 1.38,1.36 (2s, 9H).

MS: (M+H) += 461 123K (~)-(2R,3S,5R,1'S)-1-t-Butoxycarbonyl-2-aciridinyl-3-vinyl-p yrrolidine-5- carboxylic Acid t-Butyl Ester.

()- (2R, 3S, 5R, 1'S)-1-t-Butoxycarbonyl-2- (1-methanesulfonyloxy-3- azido) ethyl-3-vinyl-pyrrolidine-5-carboxylic acid t-butyl ester (2.12 g, 4.6 mmole) was reacted with triphenylphosphine (1.81 g, 6.9 mmole) in THF (30 mL) and water (7.5 mL) at 65°C for 1 hour. The reaction was concentrated in vacuo and redissolved in ethyl acetate (200 mL). The organic layer was washed with water, and brine, dried over MgS04, filtered and concentrated in vacuo. The residue was purified by chromatography on silica gel using 4% methanol in dichloromethane to provide 2 g of the crude title compound containing approximately 60% product and 40% Ph3PO which was used directly for acylation.

'H NMR (DMSO-d6) 8 5.78-5.5.98 (m, 1 H), 4.12 (d, 1H), 3.42,3.19 (2d, 1 H), 2.53-2.73 (m, 2H), 2.00-2.15 (m, 1 H), 1.68-1.76 (m, 1 H), 1.62-1.68 (m, 1 H), 1. 41 (s, 9H), 1.37,1.36 (2s, 9H).

MS: (M+H)+= 339, (M+Na) + = 361 1 23L (i)-(2R, 3S, 5R, 1'S)-1-t-Butoxycarbonyl-2-(N-acetylaziridinyl)-3-vinyl-<B R> <BR> <BR> <BR> <BR> pyrrolidine-5-carboxylic Acid t-ButvI Ester.

()- (2R, 3S, 5R,1'S)-1-t-Butoxycarbonyl-2-aziridinyl-1-vinyl-pyrroldine-5 - carboxylic acid t-butyl ester (1.03 g, 3.1 mmole) was reacted with acetic anhydride (. 42 mL, 4.7 mmole) and triethylamine (1.3 mL, 9.3 mmole) in dichloromethane (30 mL) at 25°C for 1 hours. The reaction was quenched with water (50 mL) and diluted with ethyl acetate (200 mL). The organic layer was washed with water, and brine, dried over MgS04, filtered and concentrated in vacuo. The residue was purified by chromatography on silica gel using 20% ethyl acetate/hexanes to provide the title compound (yield :. 75 g, 64%).

'H NMR (DMSO-d6) 8 5.78-5.98 (m, 1H), 5.05 (d, 1H), 4.98,4.94 (2d, 1H), 4.12-4.20 (m, 1H), 3.54,3.42 (2dd, 1H), 2.54-2.98 (m, 3H), 2.40,2.49 (2d, 1H), 2.15,2.19 (2d, 1H), 2.02,2.04 (2s, 3H), 1.68-1.82 (m, 1H), 1.42 (s, 9H), 1.48.1.45 (2s, 9H).

MS: (2M+Na) += 783 123M(+)-(2R, 3S, 5R. 1'S)-1-t-Butoxycarbonyl-2-(1-acetamido) butyl-3-vinyl-<BR> <BR> <BR> <BR> <BR> <BR> <BR> <BR> pyrrolidine-5-carboxylic Acid t-Buyl Ester.

To a suspension of copper (l) bromide-dimethyl sulfide complex (0.051g, 0.248 mmol) in THF (1.0 ml) at 0°C was added ethylmagnesium bromide (1 M) (1.0 ml, 1.0 mmol) in THF. After stirring for 10 minutes at 0 °C, a portion of this solution (0.60 ml) was added dropwise to a solution of ()- (2R, 3S, 5R, 1'S)-1-t- Butoxycarbonyl-2- (N-acetylaziridinyl)-3-vinyl-pyrrolidine-5-carboxylic acid t-butyl ester (0.020 g, 0.053 mmole) in THF (0.40 ml) at-78°C. After stirring for 20 minutes at-78°C, the reaction was warmed to 0°C and stirred for 30 minutes.

The reaction was quenched with saturated ammonium chloride (1.0 mL) and diluted with ethyl acetate (10 mL). The organic layer was washed with water and brine, dried over MgS04, filtered and concentrated in vacuo. The residue was purified by chromatography on silica gel using a gradient of 0-75% ethyl acetate/hexanes to provide the title compound (yield : 0.004 g, 19%).

'H NMR (DMSO-d6) (rotamers) 5 7.48 (d, J=9.5Hz, 1H), 5.98-5.80 (m, 1H), 5.00-4.90 (m, 2H), 4.45-4.25 (m, 1 H), 3.96-3.91 (m, 1 H), 3.60-3.57 and 3.53-3.50 (2m, 1 H), 2.91-2.76 (m, 1H), 2.59-2.42 (m, 1H), 1.80 (s, 3H), 1.73-1.59 (m, 1 H), 1.42 and 1.41 (2s, 9H), 1.40-1.15 (m, 4H), 1.37 and 1. 34 (2s, 9H), 0.89-0.82 (m, 3H) MS: (M-H)-= 409, (M+H) + = 411 123N (t)- (2R, 3S, 5R. 1'S)-2- 1-Acetamido) butyl-3-vinyl-pyrrolidine-5-carboxylic Acid Hydrochloric Acid Salt The title compound was prepared according to the method described in Example 1K, substituting ()- (2R, 3S, 5R, 1'S)-1-t butoxycarbonyl-2- (1- acetamido) butyl-3-vinyl-pyrrolidine-5-carboxylic acid t-butyl ester in place of (2R, 3R, 5R, 1'S)-2-(acetamido-3-ethyl)pentyl-3-methoxymethyl-pyrroldine- 5- carboxylic acid t-butyl ester (yield : 3.1 mg, 99%).

'H NMR (DMSO-d6) 8 8.11 (d, J=7.3Hz, 1 H), 5.76-5.69 (m, 1H), 5.16 (d, J=17. 1Hz, 1H), 5.07 (dd, J=1.5,10.3Hz, 1H), 4.30 (dd, J=7.3,9.8Hz, 1H), 4.13 (m, 1 H), 3.50 (dd, J=5.9,9.8Hz, 1 H), 2.90 (m, 1 H), 2.39 (m, 1 H), 1.92-1.85 (m, 1H), 1.87 (s, 3H), 1.52-1.18 (m, 4H), 0.85 (t, J=7.3,3H) MS: (M-H)-= 253, (M+H) + = 255 Examples 124-130 1. Organocuprate OtBu AcHN. ., OH 2. 6N HCI N HOT ICI The following title compounds were prepared in two steps according to the methods described in Examples 123M and 123N, the denoted reagents and their respective methods of preparation are substituted in place of diethylcuprate and its preparation in Example 123M for step 1.

Example 124 (~)-(2R,3S,5R,1'S)-2-(1-Acetamido)hexyl-3-vinyl-pyrrolidine- 5-carboxylicAcid Hydrochloric Acid Salt The organocuprate reagent was prepared from a Grignard reagent and a catalytic quantity of copper (l) bromide-dimethyl sulfide complex according to the methods described in Example 123M, substituting 2M butylmagnesium chloride for 1M ethylmagnesium bromide.

1H NMR (MeOD-d3) 8 5.82-5.70 (m, 1H), 5.29 (d, J=17.0Hz, 1H), 5.17 (dd, J=1.3,10.2Hz, 1H), 4.35 (dd, J=7.5,10.2Hz, 1H), 4.19 (m, 1H), 3.65 (dd, J=3.4, 9.8Hz, 1H), 3.01 (m, 1H), 2.55 (m, 1H), 2.08-1.97 (m, 1H), 2.04 (s, 3H), 1.62-1.31 (m, 8H), 0.91 (t, J=6.4Hz, 3H) MS: (M-H)-=281, (M+H) + = 283 Example 125 (~)-(2R,3S,5R,1'S)-2-(1-Acetamido-4-methyl)pentyl-3-vinyl-py rroldine-5- carboxylic Acid Hydrochloric Acid Salt The organocuprate reagent was prepared from a Grignard reagent and a catalytic quantity of copper (l) bromide-dimethyl sulfide complex according to the methods described in Example 123M, substituting iso-butylmagnesium chloride for ethylmagnesium bromide..

'H NMR (MeOD-d3) 5 5.83-5.71 (m, 1H), 5.29 (dd, J=0.7,17. 0Hz, 1H), 5.17 (dd, J=0. 7, 10. 2Hz, 1H), 4.34 (dd, J=7. 5,10. 2Hz, 1H), 4.15 (m, 1H), 3.66 (dd, J=3.4,9.8Hz, 1H), 3.01 (m, 1H), 2.55 (m, 1H), 2.08-1.97 (m, 1H), 2.04 (s, 3H), 1.65-1.10 (m, 5H), 0.91 (d, J=6.4Hz, 3H), 0.91 (d, J=6.5Hz, 3H) (M+H)+=283 Example 126 (~)-(2R,3S,5R,1'S)-2-(1-Acetamido-3,3dimethyl)butyl-3-vinyl- pyrrolidine-5- carboxylic Acid Hydrochloric Acid Salt The organocuprate reagent was prepared from a Grignard reagent and a catalytic quantity of copper (l) bromide-dimethyl sulfide complex according to the methods described in Example 123M, substituting 1M tert-butylmagnesium chloride for 1M ethylmagnesium bromide.

1H NMR (MeOD-d3) # 5.84-5.71 (m, 1H), 5.31 (d, J=17.0Hz, 1H), 5.19 (d, J=10.2Hz, 1H), 4.39-4.33 (m, 2H), 3.66 (dd, J=3.4,9.8Hz, 1H), 3.02 (m, 1H), 2.57 (m, 1H), 2.08-1.97 (m, 1H), 2.02 (s, 3H), 1.55 (dd, J=9.5,14.6Hz, 1H), 1.42 (dd, J=1.4,14.6Hz, 1H), 0.95 (s, 9H) (M+H) + = 283 Example 127 (~)-(2R,3S,5R,1'S)-2-(1-Acetamido-2-phenyl)ethyl-3-vinyl-pyr rolidine-5-carboxylic Acid Hydrochloric Acid Salt Lithium diphenylcurpate was prepared according to the method described by Lipshutz, B. H. in Organometallics in Synthesis ; Schlosser, M., Ed.; Wiley and Sons: New York, 1994; p. 292. This cuprate was used according to the methods described in Example 123M, substituting lithium diphenylcuprate for the Grignard derived diethylcuprate complex.

'H NMR (MeOD-d3) 8 7.35-7.21 (m, 5H), 5.87-5.75 (m, 1H), 5.37 (d, J=16. 6Hz, 1H), 5.26 (dd, J=1.0,10.2Hz, 1 H), 4.53 (m, 1H), 4.37 (dd, J=7.5, 9.8Hz, 1H), 3.70 (dd, J=3.7,9.8Hz, 1H), 3.11 (m, 1H), 2.97 (dd, J=6.1,14.2Hz, 1H), 2.84 (dd, J=9.5,14.2Hz, 1H), 2.59 (m, 1H), 2.08-1.99 (m, 1H), 1.93 (s, 3H) (M-H)-= 301, (M+H) += 303 Example 128 (~)-(2R,3S,5R,1'S)-2-(1-Acetamido-4-phenyl)butyl-3-vinyl-pyr roldine-5-carboxylic Acid Hydrochloric Acid Salt The organocuprate reagent was prepared from a Grignard reagent and a catalytic quantity of copper (l) bromide-dimethyl sulfide complex according to the methods described in Example 123M, substituting 1 M phenethylmagnesium chloride for 1 M ethylmagnesium bromide.

1H NMR (MeOD-d3) 8 7.29-7. 13 (m, 5H), 5.77-5.65 (m, 1H), 5.24 (d, J=16.6Hz, 1H), 5.13 (dd, J=1.0,9.8Hz, 1H), 4.33 (dd, J=7.5,10.2Hz, 1H), 4.22 (m, 1 H), 3.62 (dd, J=3.4,9.8Hz, 1 H), 2.98 (m, 1H), 2.63 (m, 2H), 2.54 (m, 1H), 2.06-1.95 (m, 1 H), 2.03 (s, 3H), 1.79-1.55 (m, 4H) (M-H)-= 329, (M+H) + = 331 Example 129 (~)-(2R,3S,5R,1'S)-2-(1-Acetamido-3-phenyl)butyl-3-vinyl-pyr roldine-5-carboxylic Acid Hvdrochloric Acid Salt The organocuprate reagent was prepared from a Grignard reagent and a catalytic quantity of copper (l) bromide-dimethyl sulfide complex according to the methods described in Example 123M, substituting 2M benzylmagnesium chloride for 1 M ethylmagnesium bromide.

'H NMR (MeOD-d3) 57. 30-7.17 7 (m, 5H), 5.82-5.70 (m, 1H), 5.28 (d, J=17.0Hz, 1H0, 5.17 (d, J=11.2Hz, 1H), 4.33 (dd, J=7.5,10.2Hz, 1H), 4.18 (m, 1H), 3.64 (dd, J=3.4,9.8Hz, 1H), 3.01 (m, 1H), 2.78 (m, 1H), 2.66-2.50 (m, 2H), 2.07 (s, 3H), 2.07-1.85 (m, 3H) (M-H)-= 315, (M+H) += 317 Example 130 <BR> <BR> <BR> (t)- (2R, 3S, 5R, 1'SL (1-Acetamido-2-propen-2-yl) ethyl-3-vinvl-pyrrolidine-5- carboxvlic Acid Trifluoroacetic Acid Salt 130A ()- (2R, 3S. 5R, 1'S)-1-t-Butoxycarbonvl-2- (N-t-Butoxycarbonylaziridinyl)-3- vinvl-ovrrolidine-5-carboxylic Acid t-Butyl Ester.

()- (2R, 3S, 5R, 1'S)-1-t-Butoxycarbonyl-2-aziridinyl-3-vinyl-pyrrolidine-5- carboxylic acid t-butyl ester (0.058 g, 0.17 mmole) was reacted with di-t- butyldicarbonate (95 mg, 0.44 mmole) and triethylamine (0.12 mL, 0.86 mmole) in dichloromethane (2.0 mL) at room temperature for 1 hour. The reaction was quenched with saturated sodium bicarbonate (1.0 mL) and diluted with ethyl acetate (20 mL). The organic layer was washed with water, and brine, dried over MgS04, filtered and concentrated in vacuo. The residue was purified by chromatography on silica gel using a gradient of 0-15% ethyl acetate/dichloromethane to provide the title compound (yield : 0.060 g, 80%).

'H NMR (DMSO-d6) (rotamers) 8 5.97-5.78 (m, 1H), 5.06-4.93 (m, 2H), 4.15 (dd, J=2.0,9.8Hz, 1H), 3.40-3.28 (m, 1H), 2.94-2.49 (m, 3H), 2.39 and 2.33 (2d, J=6.1,6.4Hz, 1 H), 2.17 and 2.11 (2d, J=3.7,3.4,1 H), 1.81-1.69 (m, 1 H), 1.42-1.36 (m, 27H) MS: (M+Na) += 461 (weak) 130B ()- (2R. 3S. 5R, 1'S)-1-t-Butoxycarbonyl-2-1-N-t-butoxvcarbonvlamino-2- propen-2-yl ethyl-3-vinvl-pyrrolidine-5-carboxvlic Acid t-Butyl Ester.

To a suspension of copper (l) bromide-dimethyl sulfide complex (0.026g, 0.127 mmol) in THF (1.0 ml) at 0 °C was added isopropenylmagnesium bromide (0.5M) (1.0 ml, 0. 50 mmol) in THF. After stirring for 10 minutes at 0 °C, the mixture was cooled to-78°C and a solution of ()- (2R, 3S, 5R, 1'S)-1-t- <BR> <BR> <BR> <BR> butoxycarbonyl-2- (N-t-butoxycarbonylaziridinyl)-3-vinyl-pyrrolidine-5-carboxy lic acid t-butyl ester (0.030 g, 0.068 mmole) in THF (1.0 ml) was added dropwise.

After stirring for 10 minutes at-78°C, the reaction was warmed to 0 °C and stirred for 2 hours. The reaction was quenched with saturated ammonium chloride (1.0 mL) and diluted with ethyl acetate (10 mL). The organic layer was washed with water, and brine, dried over MgS04, filtered and concentrated in vacuo. The residue was purified by chromatography on silica gel using a gradient of 0-10% ethyl acetate/dichloromethane to provide the title compound (yield : 0.026 g, 79%).

'H NMR (DMSO-d6) (rotamers) 8 6.64 (m, 1H), 5.96-5.76 (m, 1H), 4.98- 4.89 (m, 2H), 4.76-4.68 (m, 2H), 4.40-4.25 (m, 1H), 3.94 (m, 1H), 3.60-3.53 (m, 1H), 3.02-2.86 (m, 1 H), 2.62-2.42 (m, 1 H), 2.10-1.99 (m, 2H), 1.72 and 1.70 (2s, 3H), 1.72-1.55 (m, 1H), 1.44-1.34 (m, 27H) MS : (M-H)-= 479, (M+H) + = 481 130C t- (2R. 3S. 5R. 1'S)-1-t-Butoxycarbonyl-2- (1-N-t-butoxycarbonylacetamido- 2-propen-2-yl) ethyl-3-vinyl-pvrrolidine-5-carboxvlic Acid t-Butyl Ester.

()- (2R, 3S, 5R, 1'S)-1-t-Butoxycarbonyl-2- (1-N-t-butoxycarbonylamino-2- propen-2-yl) ethyl-3-vinyl-pyrrolidine-5-carboxylic acid t-butyl ester (0.024 g, 0.050 mmole) was reacted with lithium hexamethyldisilazide (1 M) (0.60 mL, 0.60 mmole) in THF (2.0 mL) at-25°C for 1 hour. To the above reaction was then added acetyl chloride (0.085 mL, 1.20 mmole) at-25°C and the mixture was stirred for 30 minutes. The reaction was quenched with saturated sodium bicarbonate (2.0 mL) and stirred for 30 minutes at room temperature. The reaction was diluted with ethyl acetate (20 mL). The organic layer was washed with water, and brine, dried over MgSO4, filtered and concentrated in vacuo. The residue was purified by chromatography on silica gel using a gradient of 0-15% ethyl acetate/hexanes to provide the title compound (yield : 0.015 g, 58%) along with unreacted starting material.

'H NMR (DMSO-d6) (rotamers) 8 6.01-5.84 (m, 1H), 4.99-4.89 (m, 2H), 4.76-4.58 (m, 3H), 4.33 and 4.23 (2d, J=7.8,8.1Hz, 1H), 4.13-4.04 (m, 1H), 2.69 (m, 1H), 2.62-2.42 (m, 1H), 2.29 (br s, 3H), 2.35-2.14 (m, 2H), 1.76-1.55 (m, 1 H), 1.60 (s, 3H), 1.50-1.35 (m, 27H) MS : (M+H) += 523 130D ()- (2R, 3S, 5R, 1'S)-2- 1-Acetamido-2-propen-2-yl ethyl-3-vinyl-pyrrolidine- 5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41 C, substituting ()- (2R, 3S, 5R, 1'S)-1-t-butoxycarbonyl-2-(1-N-t- butoxycarbonylacetamido-2-propen-2-yl) ethyl-3-vinyl-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- acetamido-2-hydroxy)butyl-3-(cis-propen-1-yl)-pyrroldine-5-c arbocxylic acid t-butyl ester. (yield : 12 mg, 99%).

'H NMR (MeOD-d3) 8 5.83-5.70 (m, 1H), 5.30 (dd, J=0.7,17. 0Hz, 1H), 5.19 (d, J=10.2Hz, 1H), 4.79 (s, 1H), 4.71 (s, 1H), 4.46 (m, 1H), 4.30 (dd, J=7.8, 9.8Hz, 1H), 3.66 (dd, J=3.7,9.8Hz, 1H), 3.03 (m, 1H), 2.56 (m, 1H), 2.40-2.19 (m, 2H), 2.08-1.96 (m, 1H), 2.01 (s, 3H), 1.76 (s, 3H) (M-H)-= 265, (M+H) + = 267 Examples 131-135 - 1. Organocuprate ,., OtBu AcHN. ., OH Bo 2. Li N 3. Acc H 0 4 TFA/CH2CIz TFA The following title compounds were prepared in 4 steps according to the methods described in Example 130 the denoted reagents for step 1 and their respective methods of preparation are substituted in place of isopropenyl cuprate and its preparation in 130B Example 131 ()- (2R. 3S. 5R. 1'S)-2- (1-Acetamido-1- (c/s and transLpropen-1-vl) ethyl-3-vinyl- pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The organocuprate reagent was prepared from a Grignard reagent and a catalytic quantity of copper (l) bromide-dimethyl sulfide complex according to the methods described in Example 130B, substituting 0.5M 1-propenylmagnesium bromide (mixture of cis and trans isomers) for 0.5M isopropenylmagnesium bromide.

'H NMR (MeOD-d3) (2: 1 trans : cis ratio) 8 5.81-5.54 (m, 2H), 5.43-5.30 (m, 1 H), 5.33-5.27 (m, 0.33H, cis isomer), 5.31-5.25 (m, 0.66H, trans isomer), 5.20- 5.15 (m, 1 H), 4.26-4.17 (m, 2H), 3.65 (dd, J=3.4,9.8Hz, 1 H), 2.98 (m, 1H), 2.58- 2.48 (m, 1H), 2.45-2.19 (m, 2H), 2.08-1.94 (m, 1H), 2.02 (s, 3H), 1.68 (m, 2H, trans isomer), 1.63 (m, 1 H, cis isomer) (M-H)-=265, (M+H)+=267 Example 132 ()- (2R. 3S. 5R. 1'S)-2- 1-Acetamido-2-allyl) methyl-3-vinvl-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The organocuprate reagent was prepared from a Grignard reagent and a catalytic quantity of copper (l) bromide-dimethyl sulfide complex according to the methods described in Example 130B, substituting 1 M vinylmagnesium bromide for 0.5M isopropenylmagnesium bromide.

'H NMR (MeOD-d3) 8 5.83-5.70 (m, 2H), 5.28 (d, J=17. OHz, 1H), 5.19-5.13 (m, 3H), 4.28 (m, 1H), 4.19 (dd, J=8.5,9.1Hz, 1H), 3.66 (dd, J=3.4,9.5Hz, 1H), 2.99 (m, 1 H), 2. 57-2.48 (m, 1 H), 2.44-2.26 (m, 2H), 2.05-1.93 (m, 1H), 2.01 (s, 3H) (M+H) + = 253 Exampte133 (~)-(2R, 3S. 5R. 1'S)-2- 1-Acetamido)-2- (1-buten-2-yl) ethyl-3-vinyl-pyrrolidine-5- carboxylic Acid Trifluoroacetic Acid Salt The organocuprate reagent was prepared from a Grignard reagent and a catalytic quantity of copper (l) bromide-dimethyl sulfide complex according to the methods described in Example 130B, substituting 0.5M 1-buten-2-ylmagnesium bromide for 0.5M isopropenylmagnesium bromide.

1H NMR (MeOD-d3) 8 5.81-5.73 (m, 1H), 5.30 (d, J=17.1Hz, 1H), 5.19 (d, J=10.0Hz, 1H), 4.93 (s, 1H). 4.83 (s, 1H), 4.45 (m, 1H), 4.31 (dd, J=7.6,9.8Hz, 1H), 3.69 (dd, J=3.2,9.8Hz, 1 H), 3.03 (m, 1 H), 2.59-2.53 (m, 1H), 2.38 (dd, J=5.9,14.9Hz, 1H), 2.30 (dd, J=9.5,14.9Hz, 1H), 2.07 (q, J=7.6Hz, 2H), 2.05- 1.99 (m, 1 H), 2.01 (s, 3H), 1.05 (t, J=7.6Hz, 3H) (M+H) + = 281 Example 134 ()- (2R. 3S. 5R. 1'S)-2-(1-Acetamido-2-(trans-2-buten-2-yl)ethyl-3-vinyl-pyrr olidine- 5-carboxylic Acid Trifluoroacetic Acid Salt The organocuprate reagent was prepared from a Grignard reagent and a catalytic quantity of copper (l) bromide-dimethyl sulfide complex according to the methods described in Example 130B, substituting 0.5M 1-methyl-1- propenylmagnesium bromide for 0.5M isopropenylmagnesium bromide.

1H NMR (MeOD-d3) 8 5.83-5.71 (m, 1H), 5.41 (q, J=6.8Hz, 1H), 5.31 (d, J=17. 3Hz, 1H), 5.19 (d, J=10.2-Hz, 1H), 4.42 (m, 1H), 4.31 (dd, J=7.5,9.8Hz, 1H), 3.61 (dd, J=4.0,9.8Hz, 1H), 3.01 (m, 1H), 2.62-2.52 (m, 1H), 2.46 (dd, J=9.5, 13.9Hz, 1 H), 2.26 (dd, J=5.8,13.9Hz, 1 H), 2.09-1.99 (m, 1 H), 2.00 (s, 3H), 1.72 (s, 3H), 1.59 (d, J=6.8Hz, 3H) (M+H) + = 281 Example 135 (~)-(2R, 3S. 5R. 1'S. 3'RS)-2-(1-Acetamido-3-methyl) nentyl-3-vinyl-pyrrolidine-5- carboxylic Acid Trifluoroacetic Acid Salt The organocuprate reagent was prepared from a Grignard reagent and a catalytic quantity of copper (l) bromide-dimethyl sulfide complex according to the methods described in Example 130B, substituting 2M sec-butylmagnesium bromide for 0.5M isopropenylmagnesium bromide.

1H NMR (MeOD-d3) (1: 1 mixture of methyl isomers) 8 5.82-5.69 (m, 1H), 5.27 (d, J=17. OHz, 0.5H), 5.25 (d, J=17. 0Hz, 0.5H), 5.15 (d, J=10.2Hz, 1H), 4.33 (m, 1H), 4.18 (dd, J=2.7,7.5Hz, 0.5H), 4.15 (dd, J=3.0,7.8Hz, 0.5H), 3.62 (dd, J=3.1,9.8Hz, 0.5H), 3.57 (dd, J=4.07,9.8Hz, 0.5H), 2.97 (m, 1H), 2.57-2.47 (m, 1 H), 2.03-1.92 (m, 1 H), 2.03 (s, 1.5H), 2.02 (s, 1.5H), 1.72-1.06 (m, 5H), 0.95- 0.86 (m, 6H) (M+H)+=283 Example 136 (~)-(2R,3S,5R,1'RS)-2-(1-Acetamido-1-(N-methyl-N-benzylcarba moyl)methyl-3- vinyl-pvrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 136A (i)-(2R. 3S. 5R. 1'R)-1-t-butoxycarbonyl-2-(1-acetamido-1-carboxyl)methyl-< ;BR> <BR> 3-vinyl-pyrrolidine-5-carboxylic Acid t-Butyl Ester The title compound was prepared according to the method of Example 2B substituting ()- (2R, 3S, 5R, 1'R)-1-t-butoxycarbonyl-2-(1-acetamido-1- formyl) methyl-3-vinyl-pyrrolidine-5-carboxylic acid t-butyl ester for ()- (2R, 3R, 5R, 1'S)-1-benzyl-2-(1-acetamido-3-ethyl)pentyl-3-formyl-pyrrold ine-5- carboxylic acid t-butyl ester.

136B (~)-(2R,3S,5R,1'RS)-1-t-Butoxycarbonyl-2-(1-acetamido-2-(N-m ethyl-N- benzylcarbamoyl) methyl-3-vinvl-pvrrolidine-5-carboxylic Acid t-Butyl Ester ()- (2R, 3S, 5R, 1'R)-1-t-Butoxycarbonyl-2- (1-acetamido-1-carboxyl) methyl- 3-vinyl-pyrrolidine-5-carboxylic acid t-butyl ester (36 mg, 0.09 mmole) was reacted with N-methyl-N-benzylamine (32 mg, 0.26 mmole), dimethylaminopyridine- (1mg. 0.008 mmole) and 1- (3-dimethylaminopropyl)-3- ethylcarbodiimide hydrochloride (30mg, 0.16mmole) in DMF (3 mL) at 25°C for 16 hours. The reaction was quenched with water (3 mL) and diluted with ethyl acetate (20mL). The organic layer was washed with water, and brine, dried over MgS04, filtered and concentrated in vacuo. The residue was purified by chromatography on silica gel using 50% ethyl acetate/hexanes to provide the title compound.

MS: (M+H) +=516, (M-H)-=514 136C (i)-(2R. 3S. 5R. 1'RS)-2-(1-Acetamido-1-(N-methyl-N- benzylcarbamoyl)methyl-3vinyl-pyrroldine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41C, substituting ()- (2R, 3S, 5R, 1'RS)-1-t-butoxycarbonyl-2- (1- acetamido-2- (N-methyl-N-benzylcarbamoyl) methyl-3-vinyl-pyrrolidine-5- carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl- <BR> <BR> <BR> 2- (1-acetamido-2-hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t- butyl ester (yield : 7 mg, 95%).

'H NMR (DMSO-d6) 8 8.52 (d, J=9.7HZ, 1H), 7.30 (m, 5H), 5.65 (m, 1H), 5.12 (m, 4H), 4.62 (m, 1H), 4.40 (m, 2H), 3.70 (m, 1 H), 2.90 (s, 3H), 2.20 (m, 2H), 1.96 (s, 3H), MS: (M+H) +=360, (M+23) + =382 Example 138 (i)-(2R. 3S. 5R. 1'R)-2-(1-Acetamido-2-(N-phenyl-carbonyloxy)ethyl-3-vinyl- pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 138A (~)-(2R,3S,5R,1'S)-1-t-Butoxycarbonyl-2-(1-acetamido-2-N-phe nyl- carbonyloxy) ethvl-3-vinyl-pyrrolidine-5-carboxylic Acid t-Butyl Ester ()- (2R, 3S, 5R, 1'R)-1-t-Butoxycarbonyl-2- (1-acetamido-2-hydroxy) ethyl-3- vinyl-pyrrolidine-5-carboxylic acid t-butyl ester (18 mg, 0.045 mmole) was reacted with phenylisocyanate (16 mg, 0.14 mmole) and pyridine (0. 1 ml) in THF (3 mL) at 25°C for 16 hours. The reaction was quenched with water (2 mL) and diluted with ethyl acetate (10 mL). The organic layer was washed with water, and brine, dried over MgS04, filtered and concentrated in vacuo. The residue was purified by chromatography on silica gel using 50% ethyl acetate/hexanes to provide the title compound (yield : 7.5 mg, 33%).

MS : (M+H) +=518, (M-H)-=516 138B (~)-(2R,3S,5R,1'S)-2-(1-Acetamido-1-(N-phenylcarbonyloxy)eth yl-3-vinyl- pyrroldine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41C, substituting ()- (2R, 3S, 5R, 1'R)-1-t-butoxycarbonyl-2-(1- acetamido-2-N-phenyl-carbonyloxy) ethyl-3-vinyl-pyrrolidine-5-carboxylic acid t- butyl ester in place of ()- (2R, 3S, 5R, 1'R, 1'S)-1-t-butoxycarbonyl-2-(1-acetamido- 2-hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 4 mg, 95%).

1H NMR (DMSO-d6) d 8.36 ( d, J=9.7HZ, 1H), 7.30 (m, 5H), 5.78 (m, 1H), 5.22 (m, 1H), 5. 10(m, 1H), 4.58 (m, 1H), 4.45 (m, 1H), 4.14 (, 2H), 3.58 (m, 1H), 2.88 (m, 1H), 2.27 (m, 1H), 2.12 (m, 1H), 1.88 (s, 3H) MS: (M+H) +=362, (M+23) + =384, (M-H)-=360, (M+35)-=396 Example 139 (~)-(2R,3S,5R,1'S)-2-(1-Acetamido-1-isobutyryloxy)ethyl-3-vi nyl-pyrrolidine-5- carboxvlic Acid Trifluoroacetic Acid Salt 139A (~)-(2R,3S,5R,1'S)-2'S)-1-t-Butoxycarbonyl-2-(1-acetamido-2- isobutyrvloxy) ethyl-3-vinyl-pvrrolidine-5-carboxylic Acid t-Butvl Ester ()- (2R, 3S, 5R, 1'R)-1-t-Butoxycarbonyl-2- (1-acetamido-2-hydroxy) ethyl-3- vinyl-pyrrolidine-5-carboxylic acid t-butyl ester (15 mg, 0.04 mmole) was reacted with isobutyryl chloride (8 mg, 0.08 mmole) and triethylamine (8 mg, 0.08 mmole) in dichloromethane (4 mL) at 0°C for 2 hours. The reaction was quenched with water (3 mL) and diluted with ethyl acetate (20 mL). The organic layer was washed with water, and brine, dried over MgS04, filtered and concentrated in vacuo. The residue was purified by chromatography on silica gel using 30% ethyl acetate/hexanes to provide the title compound (yield : 11 mg, 63%).

MS: (M+H) +=469, (M-H)-=467 139B (~)-(2R,3S,5R,1'R)-2-(1-Acetamido-1-isobutyryloxy)ethyl-3-vi nyl- pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41 C, substituting (~)-(2R, 3S, 5R, 1'R)-1-t-butoxycarbonyl-2-(1- acetamido-2-isobutyryloxy)ethyl-3-vinyl-pyrrolidine-5-caqrbo xylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1-acetamido-2- hydroxy) butyl-3-(cis-propen-1-yl)-pyrroldine-5-carboxylic acid t-butyl ester (yield : 6.0 mg, 96%).

1H NMR (DMSO-d6) 5 8.00 (d, J=9.9HZ, 1H), 5.63 (m, 1H), 5.08 (m, 1H), 4.98 (m, 1H), 4.35 (m, 1H), 4.25 (m, 1H), 4.08 (m, 1H), 3.55 (m, 1H), 3.45 (m, 1 H), 3.38 (m, 1H), 2.83 (m, 1H), 2.33 (m, 1H), 1.78 (s, 3H) MS : (M+H) + =243, (M+23) + =265, (M-H)-=241 Example140 (~)-(2R,3S,5R,1'R)-2-(1-Acetamido-2-N-ethyl-thiocarbonyloxy) ethyl-3-vinyl- pyrrolidine-5-carboxvlic Acid Trifluoroacetic Acid Salt 140A ~)-(2R,3S,5R,1'R)-1-t-Butoxycarbonyl-2-(1-Acetamido-2-N-ethy l- thiocarbonyloxy) ethyl-3-vinyl-pyrrolidine-5-carboxylic Acid t-Bu l Ester ()- (2R, 3S, 5R, 1'R)-1-t-Butoxycarbonyl-2- (1-acetamido-2-hydroxy) ethyl-3- vinyl-pyrrolidine-5-carboxylic acid t-butyl ester (17 mg, 0.04 mmole) was reacted with ethylisothiocyanate (19 mg, 0.21 mmole) in pyridine (2 mL) at 70°C for 17 hours. The reaction was quenched with water (3 mL) and diluted with ethyl acetate (20 mL). The organic layer was washed with water, and brine, dried over MgS04, filtered and concentrated in vacuo. The residue was purified by chromatography on silica gel using 70% ethyl acetate/hexanes to provide the title compound (yield : 10 mg, 48%).

MS : (M+H) +=486, (M+23) + =508, (M-H)-= 485 140B (~)-(2R,3S,5R,1'R)-2-(1-Acetamido-2-N-ethyl-thiocarbonyloxy) ethyl-3- vinyl-pvrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41C, substituting ()- (2R, 3S, 5R, 1'R)-1-t-butoxycarbonyl-2-(1- <BR> <BR> <BR> acetamido-2-N-ethyl-thiocarbonyloxy) ethyl-3-vinyl-pyrrolidine-5-carboxylic acid t- butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2- (1-acetamido- 2-hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 7 mg, 94%).

'H NMR (DMSO-d6) 88. 30 (d, J=9.7HZ, 1H), 5.78 (m, 1H), 5.25 (m, 1H), 5.12 (m, 1H), 4.50 (m, 1H), 4.33 (m, 1H), 4.18 (m, 2H), 3.72 (m, 1H), 3.55 (m, 2H), 2.30 (m, 1 H), 2. 10(m, 1H), 1.82 (s, 3H), 1.17 (m, 3H) MS: (M+H) + =330, (M-H)-=328 Example 141 (~)-(2R,3S,5R,1'S)-2-(1-Acetamido-2-amino)ethyl-3-vinyl-pyrr oldine-5-carboxylic AcidHydrochlorideSalt 141A (i)-(2R. 3S. 5R. 1'S)-1-t-Butoxycarbonvl-2-(1-acetamido-2-t-<BR> <BR> <BR> <BR> <BR> butoxycarbonylamino) ethyl-3-vinvl-pyrrolidine-5-carboxylic Acid t-Butyl Ester.

()- (2R, 3S, 5R, 1'S)-1-t-Butoxycarbonyl-2-(1-acetamido-2-azido)ethyl-3-vinyl - pyrrolidine-5-carboxylic acid t-butyl ester (9.5 mg, 0.022 mmole) was reacted with triphenylphosphine (23.5 mg, 0.090 mmole) in ethanol (180 L) and water (45 µL) at 70°C for 30 minutes. The reaction mixture was concentrated in vacuo. The residue was dissolved in dichloromethane (220 µL) and to it was added di-tert- butyl dicarbonate (7.3 mg, 0.034 mmol) and N, N-diisopropylethylamine (11.7 mL, 0.067 mmol) at 25°C. After 1 hour the reaction mixture was diluted with water and extracted with ethyl acetate. The organic layers were combined, washed with brine, dried over MgSO4, and concentrated in vacuo. The residue was purified by chromatography on silica gel using 100% dichloromethane to 50% dichloromethane/ethyl acetate to provide the title compound (yield : 7.5 mg, 67%).

'H NMR (DMSO-d6) (rotamers) 6 7.51 (d, J=10. 5Hz, 1 H), 6.80-6.66 (m, 1 H), 5.90-5.76 (m, 1H), 5.02-4.90 (m, 2H), 4.38-4.19 (m, 1H), 3.98-3.94 (m, 1H), 3.68- 3.62 (m, 1H), 3.09-2.73 (m, 2H), 2.60-2.42 (m, 1H), 1.80 (s, 3H), 1.72-1.62 (m, 1H), 1.42-1.34 (m, 27H).

MS: (M+H) +=498, (M+Na) +=520, (M-H)-=496, (M+CI)-=532 141B (~)-(2R,3S, 5R. 1'S)-2-(1-Acetamido-2-amino)ethyl-3-vinyl-pyrrolidine-5- carboxylic Acid DiHvdrochloride The title compound was prepared according to the method described in Example 1K, substituting ()- (2R, 3S, 5R, 1'S)-1-t-butoxycarbonyl-2-(1-acetamido- 2-t-butoxycarbonylamino) ethyl-3-vinyl-pyrrolidine-5-carboxylic acid t-butyl ester in place of () (2R, 3R, 5R, 1'S)-2-(1-acetamido-3-ethyl)pentyl-3-methoxymethyl- pyrrolidine-5-carboxylic acid t-butyl ester (yield : 3.65 mg, 99%).

1H NMR (DMSO-d6) # 8. 24 (d, J=7.9Hz, 1H), 5.75-5.68 (m, 1H), 5.16 (d, J=17.1Hz, 1H), 5.06 (d, J=10. 4Hz, 1H), 4.37-4.27 (m, 2H), 3.60-3.16 (m, 2H), 3.00- 2.88 (m, 2H), 2.46-2.36 (m, 1H), 1.91-1.81 (m, 1H), 1.86 (s, 3H).

MS: (M+H) +=242, (M+Na) +=264, (M-H)-=240, (2M-H)-=481 Example 142 (~)-(2R,3S,5R,1'S)-2-(1-Acetamido-2-acetamido)ethyl-3-vinyl- pyrrolidine-5- carboxviic Acid Hydrochloride 142A ()- (2R. 3S. 5R. 1'S)-1-t-Butoxycarbonyl-2- (1-acetamido-2-acetamido) ethyl- 3-vinyl-pvrrolidine-5-carboxviic Acid t-Butyl Ester.

()- (2R, 3S, 5R, 1'S)-1-t-Butoxycarbonyl-2- (1-acetamido-2-amino) ethyl-3- vinyl-pyrrolidine-5-carboxylic acid t-butyl ester (9.4 mg, 0.024 mmole) was reacted with acetic anhydride (11.2 µL) and triethylamine (33.1 µL) in dichloromethane (0.23 mL) at 0°C for 1 hour. The reaction was diluted with water (3 mL), extracted with ethyl acetate (12 mL), washed with brine, dried over MgS04, and concentrated in vacuo. The residue was purified by chromatography on silica gel using 100% ethyl acetate to 90% ethyl acetate/methanol to provide the title compound (yield : 6.8mg, 66%).

'H NMR (DMSO-d6) (rotamers) 8 7.79-7.74 (m, 1H), 7.54 (d, J=9.8Hz, 1H), 5.97-5.81 (m, 1 H), 5.01-4.91 (m, 2H), 4.36-4.27 (m, 1 H), 3.97-3.90 (m, 1 H), 3.68- 3.63 (m, 1 H), 3.21-3.15 (m, 1H), 3.10-2.76 (m, 1H), 2.88-2.78 (m, 1H), 2.58-2.45 (m, 1H), 1.81 (s, 3H), 1.78 (s, 3H), 1.76-1.64 (m, 1H), 1.42-1.36 (m, 18H).

MS: (M+H) +=439, (M+Na) +=462, (M-H)-=438, (M+35)-=474 142B (~)-(2R,3S,5R,1'S)-2-(1-Acetamido-2-acetamido)ethyl-3-vinyl- pyrroldine-5- carboxylic Acid Hydrochloride The title compound was prepared according to the method described in Example 1K, substituting ()- (2R, 3S, 5R, 1'S)-2- (1, 2-di-acetamido) butyl-3-vinyl- pyrrolidine-5-carboxylic acid Hydrochloridesalt in place of () (2R, 3R, 5R, 1'S)-2- (1- acetamido-3-ethyl) pentyl-3-methoxymethyl-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 3.30 mg, 80%).

MS: (M+H)+=284, (M-H)-=282, (M+CI)-=318 Example143 ()- (2R, 3S, 5R. 1'S)-2-(1-Acetamido-2-azido)ethyl-3-vinyl-pyrroldine-5-carbo xylic Acid Hydrochloride 143A ()- (2R. 3S. 5R, 1'S)-1-t-Butoxycarbonvl-2-1-N-acetamido-2-azido) ethyl-3- vinyl-pyrrlidine-5-c arboxylic Acid t-Butyl Ester.

()- (2R, 3S, 5R, 1'S)-1-t-Butoxycarbonyl-2- (N-acetylaziridinyl)-3-vinyl-pyrrolidine-5- carboxylic acid t-butyl ester (21.6 mg, 0.064 mmole) was reacted with sodium azide (41.6 mg, 0.64 mmole) and ammoniun chloride (34.2 mg, 0.64 mmol) in ethanol (270 pL) and water (30 L) at 75°C for 1 hour. The ethanol was then removed in vacuo and the remaining aqueous was extracted with ethyl acetate.

The combined organics were washed with brine, dried over MgS04, and concentrated in vacuo (crude yield : 20mg, 82%). To the crude mixture was added acetic anhydride (31 pL, 0.33 mmol) and triethylamine (92 L, 0.66 mmol) in dichloromethane (330 pL) at 0°C for 30 minutes. The reaction mixture was then concentrated in vacuo. The residue was purified by chromatography on silica gel using 100% dichloromethane to 50% dichloromethane/ethyl acetate to provide the title compound (yield : 10 mg, 60%).

'H NMR (DMSO-d6) (rotamers) 8 7.85 and 7.81 (d, J=9.5Hz and 9.8Hz, 1 H), 5.94-5.80 (m, 1 H), 5.04-4.93 (m, 2H), 4.58-4.38 (m, 1 H), 4.04-3.96 (m, 1 H), 3.72-3.66 (m, 1H), 3.41-3.21 (m, 2H), 3.09-2.79 (m, 1 H), 2.59-2.46 (m, 1 H), 1.84- 1.82 (m, 3H), 1.79-1.53 (m, 1H), 1.43-1.35 (m, 18H).

MS: (M+H) +=424, (M+Na) +=446, (2M+Na) +=869, (M-H)-=422, (M+CI)-=458 143B ()- (2R. 3S. 5R, 1'S)-2- 1-Acetamido-2-azido) ethyl-3-vinyl-pyrrolidine-5- carboxylic Acid Hydrochloric Salt.

The title compound was prepared according to the method described in Example 1K, substituting (~)-(2R,3S,5R,1'S)-1-t-butoxycarbonyl-2-(1-acetamido- 2-azido) ethyl-3-vinyl-pyrrolidine-5-carboxylic acid t-butyl ester in place of () (2R, 3R, 5R, 1'S)-2-(1-acetamido-3-ethyl)pentyl-3-methoxymethyl-pyrrolidi ne-5- carboxylic acid t-butyl ester (yield : 2.94 mg, 93%).

'H NMR (DMSO-d6) 8 8.24 (d, J=8.55Hz, 1H), 5.74-5.67 (m, 1H), 5.14 (d, J=17.1Hz, 1H), 5.06 (d, J=10.4Hz, 1H0, 4.41-4.35 (m, 2H), 3.57-3.36 (m, 3H), 2.93- 2.90 (m, 1H), 2.44-2.38 (m, 1H), 1.96-1.84 (m, 1H), 1.84 (s, 3H).

MS: (M+H) +=268, (M-H)'=266, (M+CI)-=302 Example 144 <BR> <BR> <BR> <BR> <BR> <BR> (i)-(2R. 3S. 5R. 1'S)-2-(1-Acetamido-2-N-methylamino) ethyl-3-vinyl-nvrrolidine-5- carboxvlic Acid Dihvdrochloride 144A (~)-(2R,3S,5R,1'R)-1-t-Butoxycarbonyl-2-(1-N-acetamido-2-N- methylamino)ethyl-3-vinyl-pyrroldine-5-carboxylic Acid t-Butyl Ester.

Methylamine (. 016 g,. 53 mmole) was reacted with N, O-bis- trimethylsilylacetamide (. 079 g,. 39 mmole) in DMSO (0.8 mL) at 0°C for 1 hour.

()- (2R, 3S, 5R, 1'S)-1-t-Butoxycarbonyl-2- (N-acetylaziridinyl)-3-vinyl-pyrrolidine-5- carboxylic acid t-butyl ester (. 040 g,. 11 mmole) was then reacted with the above reagent N-trimethylsilylmethylamine at 75°C for 18 hours. The reaction was diluted with ethyl acetate (7 mL) washed with water and brine, dried over magnesium sulfate, filtered and concentrated in vacuo. The residue was purified by chromatography on silica gel using chloroform-methanol-ammonia to provide the title compound (yield :. 011 g, 25%).

'H NMR (CDCl3) 5 5.78-5.98 (m, 1H), 5.90-5.04 (2m, 2H), 4.40-4.55 (brm, 1 H), 3.90-4.02 (m, 1 H), 3.64-3.75 (2m, 1 H), 2.25-2.40 (brm 3H), 2.83,2.85 (2d, 3H), 1.42,1.44 (2s, 9H), 1.34,1.37 (2s, 9H).

MS: (M+H)+=412 144B (i)-(2R. 3S. 5R. 1'S)-2- (1-Acetamido-2-N-methvlamino) ethyl-3-vinyl- pyrrolidine-5-carboxylic Acid Dihydrochloride Salt The title compound was prepared according to the method described in Example 1K, substituting ()- (2R, 3S, 5R, 1'S)-1-t-butoxycarbonyl-2-(1-acetamido- 2-N-methylamino) ethyl-3-vinyl-pyrrolidine-5-carboxylic acid t-butyl ester in place of () (2R, 3R, 5R, 1'S)-2- (1-acetamido-3-ethyl) pentyl-3-methoxymethyl-pyrrolidine- 5-carboxylic acid t-butyl ester (yield : 7.2 mg, 99%).

1H NMR (DMSO-d6) 8 8.25 (d, 1H), 5.70 (m, 1H), 5.10 (m, 2H), 4.50 (m, 1H), 4.40 (m, 1H), 2.55 (s, 3H), 1.85 (s, 3H).

MS: (M+H)+= 256 Examples 145-164 \ 1. RR'NH . O'Bu ---ACHN OH N N AcN H goc , H H O R ru 2 HCI The following title compounds were prepared according to the methods described in Examples 141-144 where R'is equal to hydrogen. Where R or R' are not equal to hydrogen the corresponding amine is used directly without the intermediacy of trimethylsilylation.

Example 145 ()-2R. 3S, 5R. 1'S)-1-Acetamido-2-N-isopropylamino) ethyl-3-vinyl-pyrrolidine- 5-carboxylic Acid Dihydroxhloride Salt 'H NMR (DMSO-d6) 5 8.30 (d, 1H), 5.70 (m, 1H), 5.10 (m, 2H), 4.40 (br, 2H), 3.52-3.68 (br, 1 H), 3.10-3.20 (br, 1 H), 2.82-2.97 (br, 1 H), 2.37-2.47 (br, 1 H), 1.88 (s, 3H), 1.25 (d, 6H).

MS: (M+H) += 284 Example 146 ()- (2R, 3S, 5R, 1'S)-2- (1-Acetamido-2-N-butylamino) ethyl-3-vinyl- pyrrolidine-5-carboxylic Acid Dihydrochloride Salt 'H NMR (DMSO-d6) 8 8.25 (d, 1H), 5.70 (m, 1H), 5.10 (m, 2H), 4.50 (m, 1 H), 4.38 (m, 1 H), 3.60 (m, 1 H), 2.90 (m, 3H), 2.40 (m, 2H), 1.87 (s, 3H), 1.62 (m, 2H), 1.33 (m, 2H), 0.90 (t, 3H).

MS: (M+H) += 298 Example 147 (~)-(2R,3S,5R,1'S)-2-(1-Acetamido-2-N-benzylamino)ethyl-3-vi nyl-pyrroldine-5- carboxylic Acid HydrochlorideSalt 'H NMR (DMSO-d6) 5 7.56-7.43 (m, 5H), 5.74-5.67 (m, 1H), 5.15-4.99 (m, 2H), 4.56 (m, 1H), 4.27-3.93 (m, 3H), 3.66-3.15 (m, 3H), 2.91-2.88 (m, 1H), 2.64- 2.34 (m, 2H), 1.86 (s, 3H).

MS: (M+H)+=332, (M+Na) +=354, (M-H)-=330, (2M-H)-=661 Example 148 (~)-(2R,3S,5R,1'S)-2-(1-Acetamido-2-N-phenythylamino)ethyl-3 -vinyl-pyrroldine- 5-carboxylic Acid Dihydrochloride Salt 1H NMR (DMSO-d6) # 8.25 (d, 1H), 7.30 (m, 5H), 5.70 (m, 1H), 5.10 (m, 2H), 4.50 (br, 1H), 4.35 (br, 1H), 3.61 (m, 1H), 3.17 (m, 3H), 2.98 (m, 3H), 2.42 (m, 1 H), 1.88 (s, 3H).

MS: (M+H) += 346 Example 149 (~)-(2R, 3S. 5R. 1'S)-2(1-Acetamido-2-N,N-dimethylamino)ethyl-3-vinyl-pyrrold ine- 5-carboxylic Acid Dihydrochloride Salt 'H NMR (DMSO-d6) 5 8.34 (d, J=9.2Hz, 1H), 5.74-5.67 (m, 1H), 5.12 (d, J=17.1Hz, 1H), 5.04 (d, J=10.4Hz, 1H), 4.67-4.62 (m, 1H), 4.40 (dd, J=7.3,10.4Hz, 1H), 3.60-3.11 (m, 3H), 2.96-2.83 (m, 1H), 2.50 (s, 6H), 2.44-2.38 (m, 1H), 1.92- 1.84 (m, 1H), 1.84 (s, 3H). MS: (M+H) +=270, (M+Na) +=292, (M-H)-=268.

Example 150 (~)-(2R,3S,5R,1'S)-2-(1-Acetamido-2-N,N-diethylamino)ethyl-3 -vinyl-pyrrolidine- 5-carboxylic Acid Dihydrochloride Salt 1H NMR (DMSO-d6) 8 8.23 (d, 1H), 5.70 (m, 1H), 5.10 (m, 2H), 4.60 (br, 1H), 4.40 (br, 1H), 3.12 (m, 4H), 2.88 (m, 1H), 2.42 (m, 1H), 1.85 (s, 3H), 1.22 (t, 3H).

MS : (M+H) += 298 Example 151 (~)-(2R,3S,5R,1'S)-2-(1-Acetamido-2-N,N-dibutylamino)ethyl-3 -vinyl-pyrroldine- 5-carboxylic Acid Dihydrochloride Salt 1H NMR (DMSO-d6) 8 8.24 (d, 1H), 5.70 (m, 1 H), 5.08 (m, 2H), 4.48-4.62 (br, 1 H), 4.28-4.43 (1 H), 3.05 (m, 4H), 2.77-2.92 (br, 1 H), 2.34-2.46 (br, 2H), 1.84 (s, 3H), 1.64 (m, 4H), 1.30 (m, 4H), 0.93 (t, 6H).

MS: (M+H)+= 354 Example 152 (~)-(2R,3S,5R,1'S)-2-(1-Acetamido-2-(N-2-hydroxyethylamino)) ethyl-3-vinyl- pyrrolidine-5-carboxylic Acid Dihydroxhloride Salt 'H NMR (DMSO-d6) 8 8.20 (d, 1H), 5.70 (m, 1 H), 5.15 (d, 1H), 5.08 (d, 1 H), 4.50 (brm, 1 H), 4.38 (brm, 1 H), 3.68 (M, 1 H), 3.0 (brm, 2H), 2.90 (m, 1 H), 2.41 (m, 1H), 1. 85 (s, 3H).

MS: (M+H) += 286 Example 153 (i (2R. 3S. 5R. 1'S)-2-(1-Acetamido-2-(N-2-hydroxyethyl-N-ethylamino))ethyl- 3- vinvpvrrolidine-5-carboxylic Acid Dihydrochloride Salt 'H NMR (DMSO-d6) 8 5.81-5.74 (m, 1H), 5.38 (d, J=17.1Hz, 1H), 5.22 (d, J=10.0Hz, 1H), 4.92-4.88 (m, 1H), 4.48 (dd, J=7.6,9.8Hz, 1H), 3.91 (t, J=4.9Hz, 2H), 3.85 (dd, J=5.6, 10.0Hz, 1H), 3.63-3.53 (m, 2H), 3.46-3.39 (m, 4H), 3.16- 3.13 (m, 1H), 2.66-2.61 (m, 1H), 2.08 (s, 3H), 2.06-2.01 (m, 1H), 1.38 (t, J=7.33, 3H).

MS: (M+H)+=314, (M+Na)+=336, (M-H)-=312, (M+CI)-=348, (2M-H)-=625 Example 154 ~)-(2R 3S. 5R. 1'S)-2-(1-Acetamido-2-(N-2-hydroxyethyl-N-propylamino))ethyl -3- vinyl-pyrrolidine-5-carboxylic Acid Dihvdrochloride Salt 1H NMR (DMSO-d6) 8 8.36 (d, J=8.5Hz, 1H), 5.75-5.68 (m, 1H), 5.13 (d, J=17.1Hz, 1H), 5.04 (d, J=10.4Hz, 1H), 4.62 (m, 1H), 4.36 (m, 1H), 3.77 (t, J=4.9Hz, 2H), 3.63-3.59 (m, 1H), 3.50-3.23 (m, 3H), 3.22-3.19 (m, 2H), 3.08 (t, J=7.3Hz, 2H), 2.91-2.87 (m, 1H), 2.44-2.39 (m, 1H), 1.99-1.88 (m, 1H), 1.84 (s, 3H), 1.75-1.70 (m, 2H), 0.90 (t, J=6.7Hz, 3H).

MS: (M+H)+=328, (M+Na)+=350, (M-H)-=326, (M+CI)-=362, (2M-H)-=653 Example 155 (~)-(2R,3S,5R,1'S)-2-(1-Acetamido-2-(imidazol-1-yl)ethyl-3-v inyl-pyrroldinet-5- carboxylic Acid DiHydrochloride rH NMR (MeOD-d3) 5. 9.06 (s, 1H), 7.72 (s, 1H), 7.58 (s, 1H), 5.84-5.76 (m, 1 H), 5.39 (d, J=17.1Hz, 1H), 5.23 (d, J=10.25Hz, 1H), 4.70-4.66 (m, 1 H), 4.52- 4.43 (m, 2H), 3.92-3.89 (m, 1H), 3.20-3.17 (m, 1H), 2.67-2.62 (m, 1H), 2.11-2.04 (m, 1 H), 1.95-1.89 (m, 1 H), 1.91 (s, 3H).

MS: (M+H) +=293, (M-H)-=291, (M+35) +=327.

Example 156 (~)-(2R,3S,5R,1'S)-2-(1-Acetamido-2-(N,N-di-(2-hydroxyethyla mino))ethyl-3-vinyl- pyrrolidine-5-carboxylic Acid Dihydrochloride Salt MS: (M+H) +=330, (M+Na) +=352, (M-H)-=328, (M+CI)-=364 Example 157 (~)-(2R,3S, 5R. 1'S)-2-(1-Acetamido-2-(N-acetyl-N-methylamino)ethyl-3-vinyl- pyrrolidine-5-carboxylic Acid Hydrochloride Salt 1H NMR (DMSO-d6) # 8.01,7.95 (2d, 1H), 5.68-5.80 (m, 1H), 5.02-5.22 (m, 2H), 4.30-4.45 (brm, 2H), 3.26,3.21 (2d, 1 H), 2.82-2.95 (brm, 1 H), 2.38-2.48 (m, 1 H), 1.98,2.02 (2s, 3H), 1.79,1.82 (2s, 3H).

MS: (M+H) += 298 Example 158 (~)-(2R,3S,5R,1'S)-2-(1-Acetamido-2-(N-2-hydroxyethyl-N-meth ylamino))ethyl-3- Vinyl-pyrrolidine-5-carboxylic Acid Dihydrochloride Salt 1H NMR (DMSO-d6) 8 8.35 (d, J=9.15Hz, 1H), 5.74-5.67 (m, 1H), 5.12 (d, J=17.1Hz, 1H), 5.04 (d, J=10. 4Hz, 1H), 4.70 (m, 1H), 4.39 (dd, J=7.3,10.4Hz, 1H), 3.80-3.75 (m, 3H), 3.61-3.43 (m, 3H), 3.23-3.16 (m, 2H), 2.91-2.82 (m, 1H), 2.82 (s, 3H), 2.44-2.39 (m, 1H), 1.92-1.84 (m, 1H), 1.84 (s, 3H).

MS: (M+H)+300, (M+Na) +=322, (2M+H-H20) +=581 Example 159 (~)-(2R,3S,5R,1'S)-2-(1-Acetamido-2-(N-propyl-N-methylamino) ethyl-3-vinyl- pyrrolidine-5-carboxylic Acid Dihydrochloride Salt 1H NMR (DMSO-d6) (broad) 5 8.3 (1H), 5.7 (1H), 5.12-5.04 (2H), 4.6 (1 H), 4.35 (1 H), 2.61-2.35 (11H), 1.9 (3H), 1.78-1.63 (2H), 1.9 (3H).

MS: (M+H)+=298, (M+Na) +=320, (M-H)-=296, (M+CI)-332, (2M-H)-=593 Example 160 (~)-(2R,3S,5R,1'S)-2-(1-Acetamido-2-(N-cyclohexyl-N-methylam ino))ethyl-3-vinyl- pyrrolidine-5-carboxvlic Acid Hydrochloride Salt 'H NMR (DMSO-d6) # 8.26 (m, 1H), 5.75-5.65 (m, 1H), 5.08 (d, J=17. 1Hz, 1H), 5.02 (d, J=10.3Hz, 1H), 4.62 (m, 1H), 4.43-4.40 (m, 1H), 3.62-3.58 (m, 1H), 3.46-3.16 (m, 2H), 2.89-2.84 (m, 1H), 2.72 (s, 3H), 2.44-2.39 (m, 1H), 2.07-1.80 (m, 5H), 1.81 (s, 3H), 1.63 (m, 1H), 1.45-1.06 (m, 6H).

MS: (M+H)+=338, (M+Na) +=360, (M-H)-=336, (M+CI)-=372 Example 161 ()- (2R, 3S, 5R. 1'S)-2- (1-Acetamido-2- (N-benzyl-N-methylamino)) ethyl-3-vinyl- pyrrolidine-5-carboxylic Acid Dihydrochloride Salt 'H NMR (DMSO-d6) 8 8.36 (m, 1H), 7.61-7.46 (m, 5H), 5.69-5.64 (m, 1H), 5.07 (d, J=17.1Hz, 1H), 4.99 (d, J=10.1Hz, 1H), 4.77 (m, 1H), 4.44-4.39 (m, 2H), 4.25 (d, J=12.9, 1H), 3.61 (m, 1H), 3.43 (m, 1H), 3.22 (m, 1H), 2.93-2.85 (m, 1H), 2.73 (s, 3H), 2.44-2.38 (m, 1H), 1.92-1.85 (m, 1H), 1.85 (s, 3H).

MS: (M+H)+=346 Example 162 (~)-(2R,3S,5R,1'S)-2-(1-Acetamido-2-(N-phenethyl-N-methylami no))ethyl-3-vinyl- pyrrolidine-5-carboxylic Acid Dihvdrochloride Salt 1H NMR (DMSO-d6) 8 8.34 (d, J=8.55Hz, 1 H), 7.37-7.26 (m, 5H), 5.76- 5.69 (m, 1H), 5.14 (d, J=17.1Hz, 1H), 5.06 (d, J=10. 4Hz, 1H), 4.72 (m, 1H), 4.46- 4.42 (m, 1H), 3.83-3.20 (m, 6H), 3.13-2.99 (m, 2H), 2.86 (s, 3H), 2.95-2.83 (m, 1H), 2.46-2.40 (m, 1H), 1.95-1.81 (m, 1H), 1.86 (s, 3H).

MS: (M+H) +=360 Example 163 (~)-(2R,3S,5R,1'R)-2-(1-Acetamido-2-(N-naphthylmethyl-N-meth ylamino))ethyl-3- vinyl-pyrrolidine-5-carboxylic Acid Dihvdrochloride Salt 1H NMR (DMSO-d6) 8 8.41 (d, J=7.3Hz, 1H), 8.32-7.59 (m, 7H), 5.60 (m, 1H), 5.04 (d, J=17.1Hz, 1H), 4.91 (d, J=9.8Hz, 1H), 4.97-4.73 (m, 3H), 4.39 (m, 1H), 3.70-3.13 (m, 3H), 2.90 (m, 1H), 2.72 (s, 3H), 2.43-2.41 (m, 1H), 2.01-1.74 (m, 1H), 1.87 (s, 3H).

MS: (M+H) +=395, (M+Na) +=418, (M-H)-=394, (M+CI)-=430, (2M-H)-=789 Example 164 (~)-(2R,3S,5R,1'S)-2-(1-Acetamido-2-(N-morphoholinyl))ethyl- 3-vinyl-pyrrolidine-5- carboxylic Acid Dihydrochloride Salt 'H NMR (DMSO-d6) 8 8.28 (d, 1 H), 5.75-5.78 (m, 1H), 5.15 (d, 1H), 5.05 (d, 1 H), 4.65 (brm, 1 H), 4.42 (m, 1 H), 3.72-3.98 (brm, 3H), 3.62 (m, 1 H), 2.90 (m, 1 H), 2.38-2.48 (m, 1 H), 1.85 (s, 3H).

MS: (M+H) += 312 Example 165 <BR> <BR> (i)-(2R, 3S, 5R 1'S. 3'R)-2-(1-Acetamido-2-(N-methyl-N-t-butylamino-N- oxide)) ethyl-3-vinyl-pyrrolidine-5-carboxylic Acid Hydrochloride Salt 165A(~)-(2R,3S,5R,1'S,3R) and (~)-(2R,3S,5R,1'S,3'S)-1-t-Butoxycarbonyl-2- (1-acetamido-2- (N-methyl-N-t-butylamino-N-oxyde))ethyl-pyrroldine-5-carboxy lic Acid t-Butyl Ester.

()- (2R, 3S, 5R, 1'S)-1-t-Butoxycarbonyl-2- (1-acetamido-2- (N-methyl-N-t- butylamino)) ethyl-3-vinyl-pyrrolidine-5-carboxylic acid t-butyl ester (37 mg,. 08 mmole) was reacted with the m-chloroperoxybenzoic acid (20 mg,. 08 mmole) in CH2CI2 (0.9 mL) at 0°C for 1 hour. The reaction was chromatographed directly on silica gel eluting with a gradient of acetone to acetone/30% MeOH to provide the title compounds isomer (~)-(2R,3S,5R,1'S, 3'R) (yield :. 010 g, 27%) and isomer ()- (2R, 3S, 5R, 1'S, 3'S) (yield :. 011 g, 29%).

165B (~)-(2R,3S,5R,1'S,3'R)-2-(1-Acetamido-2-(N-methyl-N-t-butyla mino-N- oxide)) ethvl-3-vinyl-pyrrolidine-5-carboxylic Acid Hydrochloride Salt The title compound was prepared according to the method described in Example 15C substituting ()- (2R, 3S, 5R, 1'S, 3'R)-1-t-butoxycarbonyl-2-(1- acetamido-2- (N-methyl-N-t-butylamino-N-oxide)) ethyl-pyrrolidine-5-carboxylic acid t-butyl ester for (~)-(2R,3S 5R, 1'S)-2-(1-acetamido-3-ethyl)pentyl-3- (imidazol-2-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 6 mg, 80%).

1H NMR (CD30D) 8 5.72-5.87 (m, 1H), 5.40 (d, 1H), 5.20-5.28 (m, 2H), 4.44-4.53 (dd, 1H), 3.73-3.95 (m, 3H), 3.57 (s, 3H), 3.08-3.19 (m, 1H), 2.59-2.72 (m, 1H), 2.05-2.15 (m, 1H), 2.04 (s, 3H), 1.54 (s, 9H).

MS: (M+H) += 328 Examples 166-178 = 1. MCPBA = 2. chromatographic ACHN. 14--'K OH separation-ACHN.'K OH H H I I 3. 6 NHCI O H H II R'RN R'RN HCI The following title compounds were prepared according to the method described in Example 165.

Example 166 (~)-(2R,3S,5R,1'S,3'R)-2-(1-Acetamido-2-(N-methyl-N-ispropyl amino-N- oxide)) ethyl-3-vinyl-pyrrolidine-5-carboxylic Acid Hvdrochloride Salt 1 H NMR (MeOD-d3) 8 5.87-5.74 (m, 1 H), 5.46-5.40 (m, 1 H), 5.27-5.23 (m, 1H), 5.21-5.18 (m, 1H), 4.50 (dd, J=8.1,9.8Hz, 1H), 4.04-3.87 (m, 4H), 3.54 (s, 3H), 3.20-3.14 (m, 1H), 2.69-2.60 (m, 1H), 2.12-2.01 (m, 1H), 2.05 (s, 3H), 1.50 (d, J=6.4Hz, 3H), 1.48 (d, J=6.4Hz, 3H).

MS: (M+H) +=314, (M+Na) +=336, (2M+1) += 627, (2M+Na) +=649.

Example 167 ()- (2R. 3S, 5R. 1'S. 3'S)-2- (1-Acetamido-2- (N-methyl-N-propylamino-N-oxideethyl- 3-vinyl-pyrroldine-5-carboxylic Acid HydrochlorideSalt 1H NMR (MeOD-d3) # 5.87-5.74 (m, 1 H), 5.46-5.40 (m, 1 H), 5.27-5.23 (m, 1H), 5.21-5.18 (m, 1H), 4.50 (dd, J=8.1,9.8Hz, 1H), 4.04-3.87 (m, 4H), 3.54 (s, 3H), 3.20-3.14 (m, 1H), 2.69-2.60 (m, 1H), 2.12-2.01 (m, 1H), 2.05 (s, 3H), 1.50 (d, J=6.4Hz, 3H), 1.48 (d, J=6.4Hz, 3H).

MS:(M+H)+=314, (M+H-H2O)-=295 Example 168 (~)-(2R,3S, 5R. 1'S. 3'S)-2-(1-Acetamido-2-(N-methyl-N-ethylamino-N-oxide))ethyl- 3-vinvl-nvrrolidine-5-carboxylic Acid HydrochlorideSalt 1 H NMR (MeOD-d3) 8 5.82-5.75 (m, 1H), 5.44 (d, J=17.1 Hz, 1 H), 5.26 (d, J=10. 4Hz, 1H), 5.14-5.11 (m, 1H), 4.48-4.45 (m, 1H), 4.9 (d, J=4.9Hz, 2H), 3.87 (dd, J=4.9,10.4Hz, 1H), 3.76 (q, J=6.7Hz, 2H), 3.54 (s, 3H), 3.17-3.09 (m, 1H), 2.68-2.62 (m, 1 H), 2.06 (s, 3H), 2.09-2.03 (m, 1 H), 1.45 (t, J=7.3Hz, 3H).

MS: (M+H)+=300, (M+Na) +=322, (M+H-H20) +=282 Example 169 ()- (2R. 3S. 5R. 1'S)-2- (1-Acetamido-2- (N. N-dimethylamino-N-oxide) ethyl-3-vinyl- pyrroldine-5-carboxylic Acid Hydrochloride Salt 'H NMR (DMSO-d6) 8 8.58 (d, 1H), 5.67-5.78 (m, 1H), 5.20 (d, 1H), 5.08 (d, 1 H), 4.62-4.78 (brm, 1 H), 4.25-4.42 (brm, 1 H), 4.06 (d, 1 H), 3.85-3.95 (brm, 1 H), 3.88-3.98 (brm, 1 H), 3.35-3.50 (brs, 6H), 2.36-2.48 (m, 1 H), 1.92 (m, 1 H), 1.85 (s, 3H).

MS: (M+H) += 286 Example 170 (~)-(2R,3S,5R,1'S,3'S)-2-(1-Acetamido-2-(N-methyl-N-benzylam ino-N- oxide))ethyl-3-vinyl-pvrrolidine-5-carboxylic Acid HydrochlorideSalt 1H NMR (MeOD-d3) 8 7.60-7.47 (m, 5H), 5.75-5.65 (m, 1H), 5.39 (d, J=6.35Hz, 1H), 5.21 (d, J=8.8Hz, 1H), 5.18-5.11 (m, 1H), 5.00-4.70 (m, 2H), 4.35- 4.27 (m, 1H), 4.00-3.94 (m, 2H), 3.86-3.79 (m, 1H), 3.20 (s, 3H), 3.14-3.05 (m, 1H), 2.77-2.50 (m, 1 H), 2.08 (s, 3H), 2.10-2.94 (m, 1 H).

MS: (M+H)+=362, (M+Na) +=385, (M-H)-=360, (M+35)-=396 Example 171 (~)-(2R,3S,5R,1'S,3'S)-2-(1-Acetamido-2-(N-methyl-N-t-butyla mino-N-oxoide)ethyl- 3-vinyl-pyrrolidine-5-carboxylic Acid HydrochlorideSalt 1H NMR (CD30D) 8 5.80 (m, 1H), 5.44 (d, 1H), 5.27 (d, 1H), 5.08 (m, 1H), 4.34-4.44 (dd, 1H), 3.83-3.94 (m, 3H), 3.38 (s, 3H), 3.02-3.18 (m, 1H), 2.58-2.72 (m, 1 H), 2.08 (s, 3H), 1.97-2.08 (m, 1 H), 1.55 (s, 9H).

MS: (M+H) += 328 Example 172 (~)-(2R,3S,5R,1'S,3'S)-2-(1-Acetamido-2-(N-methyl-N-isopropy lamino-N- oxide)) ethvl-3-vinyl-pyrrolidine-5-carboxylic Acid Hydrochloride Salt 1 H NMR (MeOD-d3) 8 5.86-5.74 (m, 1 H), 5.53-5.47 (m, 1 H), 5.29-5.25 (m, 1H), 5.22-5.19 (m, 1H), 4.50 (dd, J=8.1,9.5Hz, 1H), 4.13-4.04 (m, 2H), 3.96 (dd, J=4.1,10.5Hz, 1H), 3.87-3.82 (m, 1H), 3.39 (s, 3H), 3.23-3.17 (m, 1H), 2.70-2.61 (m, 1 H), 2.11 (s, 3H), 2.08-2.00 (m, 1 H), 1.50 (d, J=6.4Hz, 3H), 1.49 (d, J=6.4Hz, 3H).

MS: (M+H) +=314, (M+Na) +=336, (2M+1) +=627, (2M+Na) +=649.

Example 173 (_)-(2R. 3S, 5R, 1'S. 3'R)-2-(1-Acetamido-2-(N-methyl-N-propylamino-N- oxide))ethyl-3-vinyl-pyrrolidine-5-carboxylic Acid Hydrochloride Salt 1H NMR (MeOD-d3) 8 5.82-5.75 (m, 1H), 5.45 (d, J=17.1Hz, 1H), 5.26 (d, J=10.4Hz, 1H), 5.07-5.13 (m, 1H), 4.48-4.42 (m, 1H), 3.98 (d, J=5.5Hz, 2H), 3.86 (dd, J=4.3,9.8Hz, 1H), 3.67-3.64 (m, 2H), 3.46 (s, 3H), 3.16-3.01 (m, 1H), 2.68-2.62 (m, 1 H), 2.09-2.02 (m, 1 H), 2.06 (s, 3H), 1.92-1.86 (m, 2H), 1.04 (t, J=7. 3Hz, 3H).

MS: (M+H)+=314, (M+H-H2O)-=295 Example 174 (_)-(2R, 3S. 5R. 1'S. 3'R)-2-(1-Acetamido-2-(N-methyl-N-ethylamino-N-oxide) ethvl- 3-vinyl-pvrrolidine-5-carboxylic Acid Hydrochloride Salt 1H NMR (MeOD-d3) # 5.82-5.75 (m, 1H), 5.45 (d, J=17. 1Hz, 1H), 5.26 (d, J=10.4Hz, 1H), 5.13-5. 10(m, 1H), 4.48-4.44 (m, 1H), 4.02-3.94 (m, 2H), 3.89 (dd, J=4.3,9.8Hz, 1H), 3.82 (q, J=7.3Hz, 2H), 3.46 (s, 3H), 3.18-3. 10(m, 1H), 2.68-2.62 (m, 1H), 2.09 (s, 3H), 2.07-2.02 (m, 1H), 1.46 (t, J=7.3Hz, 3H).

MS: (M+H)+=300, (M+Na) +=322, (M+H-H2O)+=282 Example 175 (~)-(2R,3S 5R. 1'S. 3'S)-2-(1-Acetamido-2-(N-methyl-N-benzylamino-N- oxide))ethyl-3-vinyl-pyrroldine-5-carboxylic Acid HydrochlorideSalt 'H NMR (MeOD-d3) 8 7.60-7.47 (m, 5H), 5.75-5.65 (m, 1H), 5.39 (d, J=6.35Hz, 1 H), 5.21 (d, J=8.8Hz, 1 H), 5.18-5.11 (m, 1 H), 5.00-4.70 (m, 2H), 4.35- 4.27 (m, 1H), 4.00-3.94 (m, 2H), 3.86-3.79 (m, 1H), 3.40 (s, 3H), 3.14-3.05 (m, 1H), 2.77-2.50 (m, 1H), 2.08 (s, 3H), 2.10-2.94 (m, 1H).

MS: (M+H)+=362, (M+Na) +=385, (M-H)-=360, (M+35)-=396 Example 176 (~)-(2R,3S,5R,1'S)-2-(1-Acetamido-2-(N,N-diethylamino-N-oxid e))ethyl-3-vinyl- pyrrolidine-5-carboxylic Acid Hydrochloride Salt 1 H NMR (MeOD-d3) 8 5.84-5.78 (m, 1H), 5.45 (d, J=16.85Hz, 1H), 5.26 (d, J=10.0Hz, 1H), 5.09-5.05 (m, 1H), 4.45-4.42 (m, 1H), 3. 96-3. 86 (m, 3H), 3.76 (q, J=6.6Hz, 2H), 3.70 (q, J=7.3Hz, 2H), 3.15-3.11 (m, 1H), 2.68-2.62 (m, 1H), 2.08- 2.02 (m, 1H), 2.08 (s, 3H), 1.44-1.38 (m, 6H).

MS: (M+H) +=314, (M+Na) +=336, (M+2Na) +=358 Example 177 (t)- (2R,3S,5R,1'S,3'R)-2-(1-Acetamido-2-(N-pyrrolidinyl-N-oxide) )ethyl-3-vinyl- pyrrolidine-5-carboxylic Acid Hydrochloride Salt 'H NMR (DMSO-d6) # 8.74 (d, 1H), 5.65-5.80 (m, 1H), 5.28 (d, 1H), 5.10 (d, 1H), 4.82 (m, 1H), 4.40-4.50 (dd, 1H), 4.30 (d, 1H), 3.60-4.12 (brm, 5H), 2.98- 3.15 (m, 1H), 2.38-2.48 (m, 1H), 2.05-2.20 (brm, 5H), 1.88-1.98 (m, 1H), 1.87 (s, 3H).

MS: (M+H) i= 312 Example 178 (i)-(2R. 3S. 5R, 1'S)-2-(1-Acetamido-2-(N-morpholinyl-N-oxide) ethyl-3-vinVl- pyrrolidine-5-carboxylic Acid Hydrochloride Salt 'H NMR (DMSO-d6) 5 8.65 (d, 1H), 5.66-5.80 (m, 1H), 5.22 (d, 1H), 5.09 (d, 1H), 4.78 (brs, 1H), 4.32-4.42 (dd, 1H), 4.10-4.17 (brm, 2H), 3.50-4.02 (brm, 9H), 2.92-3.04 (brm, 1 H), 2.37-2.48 (m, 1 H), 1.88-1.96 (m, 1 H), 1.87 (s, 3H).

MS: (M+H) += 328 Example 179 (~)-(2R,3S,5R,1'S,3'S)-2-(1-Acetamido-2-(N-ethyl-N-methylami no-N-oxide))ethyl- 3- (cis-propen-1-vl)-pyrrolidine-5-carboxylic Acid HydrochlorideSalt 179A(_)-(2R. 3S. 5R. 1'S)-1-t-Butoxvcarbonvl-2-oxiranvl-3-(cis-propen-1-yl)-<B R> <BR> pyrrolidine-5-carboxylic Acid t-Butyl Ester.

The title compound was prepared according to the method described in Example 1231, substituting ethyltriphenylphosphonium bromide in place of methyltriphenylphosphonium bromide (yield : 350 mg, 77%).

1H NMR (CDC13) (rotamers) 8 5.55-5.43 (m, 2H), 4.13-4.04 (m, 2H), 3.14- 3.11 (m, 2H), 2.76-2.50 (m, 3H), 1.75-1.70 (m, 1H), 1.64 (d, 3H), 1.48-1.43 (m, 18H).

MS: (M+H)+=354, (M+Na)+=376, (2M+Na) +=729 179B (~)-(2R,3S,5R,1'R)-1-t-Butoxycarbonyl-2-(1-methanexsulfonylo xy-3- azido) ethyl-3-(cis-propen-1-yl)-pyrrolid ine-5-carboxylic Acid t-Butyl Ester.

The title compound was prepared according to the method described in Example 123J, substituting ()- (2R, 3S, 5R, 1'S)-1-t-butoxycarbonyl-2-oxiranyl-3.

(cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'S)-1-t-butoxycarbonyl-2- (1-acetamido) butyl-3-vinyl-pyrrolidine-5- carboxylic acid t-butyl ester (yield : 1.08 g, 84%).

'H NMR (DMSO-d6) (rotamers) 8 5.53-5.33 (m, 2H), 5.05-4.93 (m, 1H), 4.20-3.90 (m, 2H), 3.76-3.62 (m, 2H), 3.24 (s, 3H), 2.59-2.49 (m, 1H), 1.64- 1.55 (m, 5H), 1.43-1.36 (m, 18H).

MS: (M+H)+=475, (M+Na) +=497, (2M+Na) +=971 179C (ffi)-(2R, 3S. 5R,1'S)-1-t-Butoxycarbonyl-2-aziridinyl-3-(cis-propen-1-yl)- pyrrolidine-5-carboxylic Acid t-Butvl Ester.

The title compound was prepared according to the method described in Example 123K, substituting (2R, 3S, 5R, 1'S)-1-t-butoxycarbonyl-2-(1- methanesulfonyloxy-3-azido) ethyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester in place of (2R, 3S, 5R, 1'S)-1-t-butoxycarbonyl-2-(1- methanesulfonyloxy-3-azido) ethyl-3-vinyl-pyrrolidine-5-carboxylic acid t-butyl ester (crude yield : 564 mg, 71 %).

1H NMR (DMSO-d6) (rotamers) 8 5.45-5.30 (m, 2H), 4.15-3.99 (m, 1H), 3.30-3.08 (m, 1H), 3.07-2.84 (m, 1H), 2.68-2.51 (m, 1H), 2.13-1.85 (m, 1H), 1.80- 1.05 (m, 3H), 1.57 (d, J=5.4Hz, 3H), 1.41-1.35 (m, 18H).

MS: (M+H) +=352, (M+23) +=375, (2M+H) +=705, (2M+23) +=727 179D (~)-(2R,3S,5R,1'S)-1-t-Butoxycarbonyl-2-(N-acedtylaziridinyl )-3-(cis-propen- 1-yl)-pyrrolidine-5-carboxylic Acid t-Butyl Ester.

The title compound was prepared according to the method described in Example 123L, substituting ()- (2R, 3S, 5R,1'S)-1-t-butoxycarbonyl-2-aziridinyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'S)-1-t-butoxycarbonyl-2-aziridinyl-3-vinyl-pyrrolidine-5-c arboxylic acid t-butyl ester (yield : 455 mg, 72%).

'H NMR (DMSO-d6) (rotamers) 5 5.74-5.34 (m, 2H), 4.17 (dd, J=2.4, 6.35Hz, 1 H), 3.41 (dd, J=1.95,6.35Hz, 1H), 3.14-2.99 (m, 1H), 2.73-2.58 (m, 2H), 2.40 (d, J=6.35Hz, 1H), 2.17-2.12 (m, 1H), 2.05-2.00 (m, 3H), 1.66-1.55 (m, 1H), 1.56 (d, J=6.8Hz, 3H), 1.41-1.31 (m, 18H).

MS: (M+H) +=395, (M+Na) +=417, (M+H+Na) +=418, 179E (~)-(2R,3S,5R,1'S)-1-t-Butoxycarbonyl-2-(1-acetamido-2-N-eth yl-N- methylamino) ethyl-3- (cis-propen-1-vl)-pyrrolidine-5-carboxylic Acid t-Butyl Ester.

The title compound was prepared according to the method described in Example 150, substituting N-ethyl-N-methyl-amine in place of diethylamine (yield : 30 mg, 87%).

. MS : (M+H) +=454, (M+Na) +=476, (M-H)-=452, (M+35)-=488 179E (i)-(2R. 3S. 5R, 1'S. 3'R) and ()- (2R. 3S. 5R. 1'S. Butoxycarbonyl-2- (1-acetamido-2- (N-ethyl-N-methylamino-N-oxide)) ethyl-3- (cis-propen-1-yi)- pyrrolidine-5-carboxylic Acid t-Butyl Ester The title compound was prepared according to the method described in Example 165A, substituting ()- (2R, 3S, 5R, 1'S)-1-t butoxycarbonyl-2- (1- acetamido-2-(N-ethyl-N-methylamino)) ethyl-3-(cis-propen-1-yl)-pyrrolidine-5- carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'S)-1-t-butoxycarbonyl-2- (1-acetamido-2-N-methyl-N-t-butylamino) ethyl-3-(cis-propen-1-yl)-pyrrolidine-5- carboxylic acid t-butyl ester (yield : 15.2 mg, 51%).

179F (~)-(2R,3S,5R,1'S,3'R)-2-(1-Acetamido-2-(N-ethyl-N-methylami no-N- oxide)) ethvl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic Acid Hydrochloride Salt The title compound was prepared according to the method described in Example 1K, substituting (i)-(2R, 3S, 5R, 1'S, 3'R)-1-t-butoxycarbonyl-2-(1- acetamido-2- (N-methyl-N-ethyl-N-oxide)) ethyl-pyrrolidine-5-carboxylic acid t-butyl ester for (~)-(2R 3R, 5R, 1'S)-2-(1-acetamido-3-ethyl)pentyl-3-methyoxymethl- pyrrolidine-5-carboxylic acid t-butyl ester (yield : 8.7 mg, 29%).

'H NMR (MeOD-d3) 8 5.75-5.69 (m, 1H), 5.37-5.30 (m, 1H), 5.07-5.04 (m, 1 H), 4.49 (dd, J=7.8,10.2Hz, 1 H), 4.05-3.74 (m, 4H), 3.61-3.32 (m, 1 H), 3.55 (s, 3H), 2.69-2.60 (m, 1H), 2.04 (s, 3H), 1.95-1.84 (m, 1 H), 1.75 (dd, J=2.0,7.1 Hz, 3H), 1.44 (t, J=7. 1Hz, 3H).

MS: (M+H)'=314, (M+35) +=348 Examples 179-184 1.MCPBA 2. chromatographic ACHN. OH separation ACHN-Hx fOH , H H I I 3. 6 N HCI, O H H lOl R'RN-R'RN- HCI The followina title compounds were prepared according to the method described in Example 179.

Example 180 (~)-(2R,3S,5R,1'S,3'S)-2-(1-Acetamido-2-(N-ethyl-N-methylami no-N-oxide))ethyl- 3- (cis-propen-1-yl)-prrolidine-5-carboxylic Acid Hydrochloride Salt 'H NMR (MeOD-d3) 8 5.75-5.69 (m, 1H), 5.38-5.30 (m, 1H), 5.02-4.98 (m, 1 H), 4.47 (dd, J=7.8,9.8Hz, 1 H), 4.02-3.77 (m, 4H), 3.56-3.39 (m, 1 H), 3.47 (s, 3H), 2.69-2.59 (m, 1H), 2.07 (s, 3H), 1.95-1.84 (m, 1H), 1.76 (dd, J=1.7,7. 1Hz, 3H), 1.46 (t, J=7. 1Hz, 3H).

MS: (M+H)+=314, (M+35) +=348 Example 181 )- (2R, 3S, 5R. 1'S, 3'R)-2- (1-Acetamido-2- (N-isopropvl-N-methylamino-N- oxide)) )ethyl-3-(cis-propen-1-yl)-pyrroldine-5-carboxylic Acid Hydrochloride Salt 'H NMR (MeOD-d3) 8 5.76-5.66 (m, 1 H), 5.39-5.31 (m, 1 H), 5.17-5.11 (m, 1H), 4.51 (dd, J=7.5,10.2Hz, 1H), 4.07-3.76 (m, 4H), 3.55 (S, 3H), 3.52-3.39 (m, 1H), 2.69-2.60 (m, 1H), 2.02 (S, 3H), 2.08-1.84 (m, 1H), 1.75 (dd, J=1.7,7. 1Hz, 3H), 1.50 (d, J=6. 1Hz, 3H), 1.48 (d, J=6.4Hz, 3H).

MS: (M+H)+=314, (M+35) +=348 Example 182 (~)-(2R, 3S. 5R. 1'S. 3'S)-2-(1-Acetamido-2-(N-isopropyl-N-methvlamino-N- oxide))ethyl-3-(cis-propen-1-yl)-pyrroldine-5-carboxylic Acid Hydrochloride Salt 'H NMR (MeOD-d3) 8 5.76-5.68 (m, 1H), 5.39-5.31 (m, 1H), 5.10-5.05 (m, 1H), 4.49 (dd, J=7.8,9.8Hz, 1H), 4.12-3.84 (m, 4H), 3.55-3.44 (m, 1H), 3.41 (S, 3H), 2. 69-2.60 (m, 1H), 2.08 (S, 3H), 2.07-1.84 (m, 1H), 1.76 (dd, J=1.7,6.8Hz, 3H), 1.51 (d, J=2.4Hz, 3H), 1.49 (d, J=2.4Hz, 3H).

MS: (M+H) +=314, (M+35) +=348 Example 183 (~)-(2R,3S, 5R. 1'S. 3'S)-2- (1-Acetamido-2- (N-isobuyl-N-methylamino-N- <BR> <BR> <BR> oxide)) ethyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic Acid Hydrochloride Salt 'H NMR (MeOD-d3) 8 5.75-5.69 (m, 1H), 5.38-5.31 (m, 1H), 5.18-5.12 (m, 1 H), 4.53 (dd, J=7.5,9.8Hz, 1H), 4.25-3.42 (m, 6H), 3.65 (s, 3H), 2.68-2.58 (m, 1H), 2.44-2.36 (m, 1H), 2.05 (s, 3H), 1.94-1.87 (m, 1H), 1.76 (d, J=2.7Hz, 3H), 1.14 (d, J=6.8Hz, 6H).

MS: (M+H) +=342, (M+Na) +=364, (M-H)-=340 Example184 ()- (2R. 3S. 5R, 1'S. 3'R)-2-y1-Acetamido-2- (N-isobuyl-N-methvlamino-N- oxide))ethyl-3-(cis-propen-1-yl)-pyrroldine-5-carboxylic Acid Hydrochloride Salt 'H NMR (MeOD-d3) 8 5.75-5.69 (m, 1 H), 5.38-5.31 (m, 1 H), 5.06-5.02 (m, 1 H), 4.48 (dd, J=7.5,9.8Hz, 1H), 4.08-3.85 (m, 3H), 3.70-3.57 (m, 2H), 3.52 (s, 3H), 3.48-3.41 (m, 1H0, 2.70-2.60 (m, 1H), 2.40-2.36 (M, 1H), 2.08 (s, 3H), 1.95- 1.84 (m, 1H), 1.75 (dd, J=1.7,7. 1Hz, 3H), 1.14 (d, J=6.8Hz, 6H).

MS: (M+H) +=342, (M+Na) +=364, (M-H)'=340 Example 185 <BR> <BR> <BR> () (2R,3S. 5R, 1'S)-2- 1-Acetamido-2- (N-isopropyl-N-hydroxyamino)) ethyl-3-vinrl- pyrrolidine-5-carboxylic Acid HydrochlorideSalt 165A (~)-(2R,3S,5R,1'S)-1-t-Butoxycarbonyl-2-(1-acetamido-2-(N-is opropyl-N- hydroxyamino)) ethyl-3-vinvl-pvrrolidine-5-carboxYlic Acid t-Butvl Ester.

(~)-(2R,3S,5R,1'S)-1-t-Butoxycarbonyl-2-(1-acetamido-2- isopropylamino) ethyl-3-vinyl-pyrrolidine-5-carboxylic acid t-butyl ester (21 mg, 0.048 mmole) was dissolved in 0.95 mL of acetone. It was then titrated with 0.14 mL of a solution of dimethyldioxirane (0.1 M) in acetone at-45°C for 0.5 hour.

The reaction was stopped by concentrating the mixture in vacuo. The residue was purified by chromatography on silica gel using 100% dichloromethane to 90% dichloromethane/methanol to provide the title compound (yield : 5.3 mg, 24%) and recovered starting material (yield 12 mg, 57%).

1 H NMR (MeOD-d3) 8 5.95-5.89 (m, 1 H), 5.08-4.94 (m, 2H), 4.75-4.68 (m, 1 H), 4.13-3.83 (m, 2H), 2.85-2.47 (m, 4H), 1.96 (s, 3H), 1.82-1.76 (m, 1H), 1.52- 1.44 (m, 18H), 1.45-1.29 (m, 1H), 1.07-1.04 (m, 6H).

MS: (M+H) +=456, (M+Na) +=478, (M-H)-=454, (M+35)-=490.

185B (~)-(2R,3S,5R,1'S)-2-(1-Acetamido-2-(N-isopropyl-N-hydroxyam ino))ethyl- 3-vinyl-pyrrolidine-5-carboxylic Acid Hydrochloride Salt The title compound was prepared according to the method described in Example 1K, substituting ()- (2R, 3R, 5R, 1'S)-1-t-butoxycarbonyl-2- (1-acetamido- <BR> <BR> <BR> <BR> 2- (N-isopropyl-N-hydroxyamino)) ethyl-3-vinyl-pyrrolidine-5-carboxylic acid t-butyl ester in place of () (2R, 3R, 5R, 1'S)-2-(1-acetamido-3-ethyl)pentyl-3- methoxymethyl-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 3.0 mg, 87%).

1H NMR (MeOD-d3) 8 5.83-5.71 (m, 1H), 5.40 (d, J=17. 3Hz, 1 H), 5.24 (d, J=10.2Hz, 1H), 4.48 (dd, J=7.8,10.2Hz, 1H), 3.88-3.59 (m, 4H), 3.17-3.10 (m, 1 H), 2.67-2.58 (m, 1H), 2.10-1.99 (m, 1H), 2.09 (s, 3H), 1.33-1.17 (m, 1H), 1.38 (d, J=6.4Hz, 6H).

MS: (M+H)+=300,(M-H)-=298, (2M-H)-=597 Example 186 (~)-(2R,3S,5R,1'R)-2-(1-Acetamido-2-oxo-2-pyhenyl_ethyl-3-(c is-propen-1-yl)- pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41C, substituting ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- <BR> <BR> <BR> acetamido-2-oxo-2-phenyl) ethyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- acetamido-2-hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 5.9 mg, 100%).

'H NMR (DMSO-d6) 8 8.62 (d, J= 9.8Hz, 1H), 7.93 (m, 2H), 7.68 (m, 1H), 7.55 (t, J= 7.9Hz, 2H), 5.61 (m, 1H), 5.48 (m, 1H), 5.19 (m, 1H), 4.50 (m, 1H), 3.98 (t, J= 9.8Hz, 1H), 3.30 (m, 1H), 2.38 (m, 1H), 1.73 (m, 1H), 1.71 (s, 3H), 1.59 (m, 3H).

MS : M+N)+= 331, (M+Na) += 353, (M-H)-= 329.

Example 187 (~)-(2R,3S,5R,1'R2'R)-2-(1-Acetamido-2-methyoxy-4-vinyl)buty l-3-(cis-propen-1- yl)pyrroldine-5-carboxylic Acid Trifluoroacetic Acid Salt.

187A (~)-(2R,3S,5R,1'R,2'R)-1-t-Butoxycarbonyl-2-(1-acetamido-2-m ethyoxy-4- vinyl) butyl-3- (cis-propen-1-yl)-pvrrolidine-5-carboxylic Acid t-Butyl Ester.

The title compound is prepared according to the method described in Example 84A, substituting ()- (2R, 3S, 5R, 1'R, 2'R)-1-t-butoxycarbonyl-2-(1- acetamido-2-hydroxy4-vinyl) butyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester for ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2- (1-acetamido-2- hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester.

187B (~)-(2R,3S,5R,1'R,2'R)-2-(1-Acetamido-2-methyoxhy-4-vinyl)bu tyl-3-(cis- propen-1-yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound is prepared according to the method described in Example 41C, substituting ()- (2R, 3S, 5R, 1'R, 2'R)-1-t-butoxycarbonyl-2-(1- acetamido-2-methoxy-4-vinyl) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2- (1- acetamido-2-hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester.

Example188 <BR> <BR> <BR> (t)- (2R. 3S, 5R, 1'R, 2'S)-2- 1-Acetamido-2-methoxy-4-vinyl) butyl-3- (cispropen-1- yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt.

188A (~)-(2R,3S,5R,1'R,2'S)-1-t-Butoxycarbonyl-2-(1-acetamido-2-m ethyoxy-4- vinyl) butyl-3-(cis-propen-1-yl)-ovrrolidine-5-carboxylic Acid t-Butvl Ester.

The title compound was prepared according to the method described in Example 84A, substituting ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- acetamido-2-hydroxy-4-vinyl) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl esterfor ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2- (1-acetamido-2- hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 0.0044 g, 22%).

MS: (M+H) +=481, (M-H)'=479.

188B(t)-(2R, 3S, 5R, 1'R, 2'S)-2-(1-Acetamido-2-methoxy4-vinvl) butyl-3-(cis- propen-1-vl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41C, substituting ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- acetamido-2-methoxy-4-vinyl) butyl-3-(cis-propen-1-yl)-pyrroldine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- acetamido-2-hydroxy)butyl-3-(cis-propen-1-yl)-pyrrolidine-5- carboylic acid t-butyl ester (yield : 0.0031 g, 100%). oh NMR (DMSO-d6) 8 7.93 (d, J=9.2Hz, 1H), 5.81 (m, 1H), 5.49 (m, 1H), 5.26 (m, 1H), 5.1-4.9 (m, 2H), 4.29 (m 1H), 4.03 (m, 2H), 3.68 (m, 1H), 3.26 (m, 1H), 3.25 (s, 3H), 3.18 (quint., J=8.5Hz, 1H), 2.40 (dt, J=12. 7,7.3Hz, 1H), 2.32 (M, 1H), 2.20 (m, 1H), 2.02 (m, 1H), 1.85 (s, 3H), 1.68 (m, 1H), 1.64 (m, 1H), 1.61 (dd, J=6.7,1.8Hz, 3H), 1.55-1.40 (m, 2H).

MS: M+H)+=325, (M+Na) +=347, (M-H)-=323.

Example 189 ()- (2R, 3 1H NMR (DMSO-d6) (rotamers) 8 7.62-7.58 (m, 4H), 7.49-7.38 (m 6H), 4.97-4.79 (m, 1H), 4.19-4.02 (m, 2H), 3.79-3.48 (m, 2H), 3.15 and 3.13 (2s, 3H), 2.49-2.39 (m, 2H), 1.98-1.74 (m, 1H), 1.43-1.25 (m, 18H), 1.02 and 1.00 (2s, 9H) MS: (M+H)'= 703, (M+Na) + = 725 189B ~)-(2R,3S,5R,1'S)-1-t-butoxycarbonyl-2-aziridinyl-3-t- butyldiphenylsilyloxymethyl-pyrroldine-5-carboxylic acid t-butyl ester The title compound was prepared according to the method described in Example 123K substituting ()- (2R, 3R, 5R, 1'S)-1-t-butoxycarbonyl-2- (1- methanesulfonyloxy-3-azido) ethyl-3-t-butyldiphenylsilyloxymethyl-pyrrolidine-5- carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'S)-1-t-butoxycarbonyl-2-(1- (1-methanesulfonyloxy-3-azido) ethyl-3-vinyl-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 5.9 g, 79%).

'H NMR (DMSO-d6) (rotamers) 8 7.60-7.56 (m, 4H), 7.49-7.39 (m 6H), 4.11-4.05 (m, 1 H), 3.67-3.48 (m, 2H), 3.42-3.30 (m, 1H), 2.49-2.39 (m, 1H), 2.25- 1.61 (m, 5H), 1.40,1.35,1.33, and 1.27 (4s, 18H), 0.99 and 0.98 (2s, 9H) MS: (M+H)+=581, (M+Na) + = 603 189C (t)- (2R. 3R, 5R. 1'S)-1-t-Butoxvcarbonvl-2-N-acetlaziridinyl-3-t- butvldiphenylsilyloxymethyl-pyrrolidine-5-carboxylic Acid t-Butyl Ester The title compound was prepared according to the method described in Example 123L substituting ()- (2R, 3R, 5R, 1'S)-1-t-butoxycarbonyl-2-aziridinyl-3-t- butyidiphenylsilyloxymethyl-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'S)-1-t-butoxycarbonyl-2-aziridinyl-3-vinyl-pyrrolidine-5-c arboxylic acid t-butyl ester (yield : 3.1 g, 96%).

1H NMR (DMSO-d6) (rotamers) 8 7.60-7.57 (m, 4H), 7.49-7.39 (m 6H), 4.18-4.11 (m, 1H), 3.71-3.51 (m, 3H), 2.76-2.68 (m, 1H), 2.58-2.45 (m, 1H), 2.46 and 2.39 (2d, J=6.1,6. 1Hz, 1H), 2.40 and 2.47 (2m, 1H), 2.08 and 2.05 (2d, J=3.1,3.1 Hz, 1H), 2.02 and 1.99 (2s, 3H), 1.94-1.79 (m, 1H), 1.41,1.36,1.35 and 1.29 (4s, 18H), 0.99 and 0.98 (2s, 9H) MS: (M+H)'= 623, (M+Na) + = 645 189D ()- (2R. 3R. 5R, 1'R)-1-t-Butoxvcarbonyl-2- (1-acetamido-2-acetoxy) ethyl-3-t<BR> <BR> butyldiphenylsilyloxymethyl_pyrroldine-5-carboxylic Acid t-Butyl Ester ()- (2R, 3R, 5R, 1'S)-1-t-butoxycarbonyl-2-N-acetylaziridinyl-3-t- butyldiphenylsilyloxymethyl-pyrrolidine-5-carboxylic acid t-butyl ester (2.75g, 4.40 mmole) was reacted with potassium acetate (2.49 g, 25.37 mmole) and acetic acid (1.45 mL, 25.37 mmole) in DMSO (45 mL) at 100°C for 16 hours. The reaction was quenched with 1 N NaHC03 (100 mL) and diluted with ethyl acetate (300 mL). The organic layer was washed with water, and brine, dried over MgS04, filtered and concentrated in vacuo. The residue was purified by chromatography on silica gel using 100% dichloromethane to 50% dichloromethane/ethyl acetate to provide the title compound (yield : 2.45g, 81 %).

MS: (M+H)+=683, (M+Na) +=705, (M-H)-=681, (M+CI)-=717 189E()- (2R, 3R, 5R. 1'R)-1-t-Butoxycarbony1-acetamido-2-hydroxy ethyl-3-t-<BR> <BR> <BR> butvldiphenylsilyloxvmethyl-pyrrolidine-5-carboxylic Acid t-Butyl Ester ()- (2R, 3R, 5R, 1'R)-1-t-Butoxycarbonyl-2- (1-acetamido-2-acetoxy) ethyl-3-t- butyidiphenylsilyloxymethyl-pyrrolidine-5-carboxylic acid t-butyl ester (2.45 g, 3.58 mmole) was reacted with potassium carbonate (1.48 g, 10.73 mmole) in methanol (18 mL) and THF (18mL) at 25°C for 45 minutes. The reaction was quenched with water (100 mL) and diluted with ethyl acetate (200 mL). The organic layer was washed with water, and brine, dried over MgS04, filtered and concentrated in vacuo. The residue was purified by chromatography on silica gel using 85% dichloromethane/ethyl acetate to 100% ethyl acetate to provide the title compound (yield : 2.05 g, 90%).

MS: (M+H) +=641, (M+Na) +=663, (2M+Na+H) +=1304, (M-H)-=639, (M+CI)- =675 189F (~)-(2R,3S,5R,1'R)-1-t-Butoxycarbonyl-2-(1-acetamido-2-formy l)ethyl-3-t- butyldiphenylsilyloxymethyl-pyrrolidine-5-carboxylic Acid t-Butyl Ester The title compound was prepared according to the method described in Example 41A substituting ()- (2R, 3R, 5R, 2'R)-1-t-butoxycarbonyl-2-(1-acetamido- 2-hydroxy)ethyl-3-t-butyldiphenylsilyoxymethl-pyrroldine-5-c arboxylic acid t- butyl ester in place of ()- (2R, 3S, 5R, 1'R)-1-t-butoxycarbonyl 2- (1-acetamido-2- hydroxy) ethyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester.

'H NMR (DMSO-d6) (rotamers) 9.49 (d, J=16.3,1H), 8.33 and 8.29 (2d, J=8.8 and 8.8Hz, 1 H), 7.58-7.38 (m, 10H), 4.94 and 4.84 (2dd, J=4.4,8.8Hz and 4.4,8.8Hz, 1H), 4.26-3.37 (m, 4H), 2.47-2.30 (m, 1 H), 1.97-1.83 (m, 1H), 1.92 (s, 3H), 1.42-1.18 (m, 18H), 1.42-1.18 (m, 1 H), 1.00-0.97 (m, 9H).

MS: (M+H) +=639, (M-H)-=637 189G (~)-(2R,3S,5R,1'S)-1-t-Butoxycarbonyl-2-(1-acetamio-1-vinyl) methyl-3-t- butrldiphenYlsilyloxymethyl-pyrrolidine-5-carboxylic Acid t-Butyl Ester The title compound was prepared according to the method described in Example 118A substituting ()- (2R, 3R, 5R, 1'R)-1-t-butoxycarbonyl-2-(1- acetamido-2-formyl) ethyl-3-t-butyldiphenylsilyloxymethyl-pyrrolidine-5-carboxyl ic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R)-1-t-butoxycarbonyl 2- (1- acetamido-2-formyl) ethyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester.

1H NMR (DMSO-d6) (rotamers) 7.99-7.74 (m, 1H), 7.59-7.39 (m, 10H), 5.80-5.68 (m, 1H), 5.21-5.01 (m, 3H), 3.97-3.31 (m, 1H), 3.78-3.74 (m, 1H), 3.60- 3.46 (m, 2H), 2.53-2.37 (m, 1H), 2.09-1.72 (m, 1H), 1.87 (s, 3H), 1.42-1.23 (m, 19H), 1.00-0.99 (m, 9H).

189H (~0-(2R,3R,5R,1'R,2'R) and (i)-(2R, 3R. 5R. 1'R, 2'S)-1-t-Butoxycarbonyl-2- (1-acetamido-2,3-dihydroxy)propyl-3-t-butyldiphenylsilyloxym ethyl-pyrrolidine-5- carboxylic Acid t-Butyl Ester The title compounds were prepared according to the method described in Example 20A substituting ()- (2R, 3R, 5R, 1'S)-1-t-butoxycarbonyl-2-(1-acetamido- <BR> <BR> <BR> 2-vinyl) ethyl-3-t-butyldiphenylsilyloxymethyl-pyrrolidine-5-carboxyl ic acid t-butyl ester in place of ()- (2R, 3S, 5R0-1-benzyl-2-vinyl-3-t-butyldimethylsilyloxymethyl- pyrrolidine-5-carboxylic acid t-butyl ester ()- (2R, 3R, 5R, 1'R, 2'S) isomer (yield : 311mg, 24%) ()- (2R, 3R, 5R, 1'R, 2'R) isomer (yield : 700 mg, 54%).

(i)-(2R, 3R, 5R, 1'R, 2'S)'H NMR (DMSO-d6) (rotamers) 7.62-7.39 (m, 11 H), 4.56 and 4.51 (d, J=4.8,1 H), 4.46-4.39 (m, 2H), 3.97-3.82 (m, 1 H), 3.74- 3.47 (m, 3H), 3.28-3.21 (m, 2H), 2.89-2.64 (m, 1H), 2.51-2.45 (m, 1H), 2.05-1.8 (m, 1H), 1.87-1.86 (m, 3H), 1.43-1.23 (m, 19H), 0.99-0.98 (m, 9H).

()- (2R, 3R, 5R, 1'R, 2'R)'H NMR (DMSO-d6) (rotamers) 7.63-7.40 (m, 11 H), 4.56-4.54 (d, J=4.8,1 H), 4.47-4.33 (m, 2H), 3.94-3.80 (m, 1 H), 3.85-3.80 (m, 1 H), 3.76-3.68 (m, 1H), 3.60-3.51 (m, 1H), 3.44-3.35 (m, 1H), 3.30-3.21 (m, 1H), 2.78-2.62 (m, 1 H), 2.46-2.31 (m, 1H), 2.07-1.98 (m 1 H), 1.83 (s, 3H), 1.39-1.29 (m, 19H), 1.00-0.99 (m, 9H).

1891 (~)-(2R,3R,5R,1'R,2'S)-1-t-Butoxycarbonyl-2-(1-acetamido-1-( 2, 2- dimethyl-1. 3-dioxolan-4-vl)) methyl-3-t-buyldiphenylsyloxymethyl-pvrrolidine-5- carboxylic Acid t-Butyl Ester ()- (2R, 3R, 5R, 1'R, 2'R)-1-t-Butoxycarbonyl-2-(1-acetamido-2, 3- <BR> <BR> <BR> <BR> d ihyd roxy) propyl-3-t-butyidiphenylsilyloxymethyl-pyrrolidine-5-carboxy lic acid t- butyl ester was reacted with 2,2-dimethoxypropane (1.1 ml, 9.09 mmole) and p- Toluenesulfonic acid (4.3 mg, 0.023 mmole) in tetrahydrofuran (4.5 mL) at 25°C for 45 minutes. The reaction was quenched with triethylamine (3 mL). Stirring was continued for an additional 10 minutes. The reaction was then diluted with 10% NaHC03 (15 mL) and extracted with ethyl acetate (45 ml). The organic layer was washed with water, and brine, dried over MgS04, filtered and concentrated in vacuo. The residue was carried over to the next step. purified by chromatography on silica gel using 100% dichlormethane to 94% dichloromethane/methanol to provide the title compound (yield : 194 mg, 91%).

189J (~)-(2R,3R, 5R. 1'R. 2'S)-1-t-B utoxvcarbonyl-2-(1-acetamido-1-(2, 2- dimethyl-1.3-dioxolan-4-yl))methyl-3-hydroxymethyl-pyrrolidi ne-5-carboxylic Acid t-Butyl Ester The title compound was prepared according to the method described in Example 123G substituting ()- (2R, 3R, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- acetamido-1-(2,2-dimethyl-1,3-dioxolan-4-yl))methyl-3-t- butyidiphenylsilyloxymethyl-pyrrolidine-5-carboxylic acid t-butyl ester (~)- (2R, 3R, 5R, 1'S)-1-t-butoxycarbonyl-2-oxiranyl-3-t-butyidiphenylsilyloxy methyl- pyrrolidine-5-carboxylic acid t-butyl ester. The resulting residue was purified by chromatography on silica gel using 100% dichlormethane to 94% dichloromethane/methanol to provide the title compound (yield : 194 mg, 91%).

189JJ(i)-(2R, 3R, 5R. 1'R. 2'S)-1-t-Butoxycarbonyl-2-(1-acetamido-1-(2, 2- dimethvl-1, 3-dioxolan-4-vl)) methyl-3-formyl-pyrrolidine-5-carboxic Acid t-Butyl Ester The title compound was prepared according to the method described in Example 123H substituting ()- (2R, 3R, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- acetamido-1-(2,2-dimethyl-1,3-dioxolan-4-yl)methyl-3-hydroxy methyl-pyrrolidine- 5-carboxylic acid t-butyl ester for (i)-(2R, 3R, 5R, 1'S)-1-t-butoxycarbonyl-2- oxiranyl-3-hydroxymethyl-pyrrolidine-5-carboxylic acid t-butyl ester.

189K (~)-(2R,3S,5R,1'R,2S)-1-t-Butoxycarbonyl-2-(1-acetamido-1-(2 ,2-dimethyl- 1,3-dioxolan-4-yl))methyl-3-(cis-propen-1-yl))-pyrrolidine-5 -carboxylic Acid t-Butyl Ester The title compound was prepared according to the method described in Example 35A substituting ()- (2R, 3R, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- acetamido-1-(2,2-dimethyl-1,3-dioxolan-4-yl))methyl-3-formyl -pyrrolidine-5- carboxylic acid t-butyl ester in place of ()- (2R, 3R, 5R, 1'S)-1-t-butoxycarbonyl-2- (1-acetamido-3-methyl) butyl-3-formyl-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 11.5 mg, 59%).

1H NMR (CDCl3) : 5 6.62 (d, 1H), 5.56 (m, 1H), 5.40 (m, 1H), 4.43 (m, 1 H), 4.25 (m, 1 H), 4.16 (m, 1H), 4.02 (m, 1H), 3.88 (m, 1H), 3.54 (m 1H), 3.14 (m, 1 H), 2.54 (m 1 H), 2.04 (s, 3H), 1.71 (m 1 H), 1.60 (dd, 3H), 1.46 (s, 9H), 1.45 (s, 9H), 1.40 (s, 3H), 1.32 (s, 3H).

MS: (M+H) +=483 189L (~)-(2R,3S,5R,1'R,2'S)-2-(1-Acetamido-2,k3-dihydroxy)propyl- 3-(cis- propen-1-yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41C, substituting (~)-(2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- acetamido-1- (2, 2-dimethyl-1, 3-dioxolan-4-yl)) methyl-3- (cis-propen-1-yl)- pyrrolidine-5-carboxylic acid t-butyl ester in place ()- (2R, 3S, 5R, 1'R, 2'S)-1-t- butoxycarbonyl-2- (1-acetamido-2-hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5- carboxylic acid t-butyl ester.

'H NMR (DMSO-d6) : # 7.84 (d, J=9Hz, 1H), 5.49 (m, 1H), 5.27 (m, 1H), 4.47 (m, 1H), 4.25 (m, 1H), 4.17 (m, 1H), 3.75 (m, 1H), 3.59 (m, 1H), 3.35 (m, 1 H), 3.18 (m, 1H), 2.43 (m, 1 H), 1.81 (s, 3H), 1.55 (dd, 3H).

MS: (M+H)+=287 Example 190 <BR> <BR> ()- (2R. 3R. 5R. 1'R. 2'R)-2- (1-Acetamido-2. 3-dihydroxy) propyl-3- (cis-propen-1-yl)- pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 190A (~)-(2R,3R,5R,1'R,2'R)-1-t-Butoxycarbonyl-2-(1-acetamido-1-( 2,2- dimethyl-1,3-dioxolan-4-yl)methyl-3-t-butyldiphenylsilyloxym ethyl-pyrrolidine-5- carboxylic Acid t-Buyl Ester The title compound was prepared according to the method described in Example 1891 substituting (~)-(2R, 3R, 5R, 1'R, 2'R)-1-t-butoxycarbonyl-2-(1- <BR> <BR> <BR> acetamido-2, 3-dihydroxy) propyl-3-t-butyldiphenylsilyloxymethyl-pyrrolidine-5- carboxylic acid t-butyl ester in place of ()- (2R, 3R, 5R, 1'R, 2'S)-1-t- Butoxycarbonyl-2- (1-acetamido-2, 3-dihydroxy) propyl-3-t- butyldiphenylsilyloxymethyl-pyrrolidine-5-carboxylic acid t-butyl ester.

190B (~)-(2R,3R,5R,1'R,2'R0-1-t-Butoxycarbonyl-2-(1-acetamido-1-( 2,- <BR> <BR> dimethVl-1. 3-dioxolan-4-yl)) methvl-3-hydroxvmethyl-pyrrolidine-5-carboxylic Acid t-Butyl Ester The title compound was prepared according to the method described in Example 123G substituting ()- (2R, 3R, 5R, 1'R, 2'R)-1-t-butoxycarbonyl-2-(1- acetamido-1- (2, 2-dimethyl-1, 3-dioxolan-4-yl)) methyl-3-t- butyldiphenylsilyloxymethyl-pyrrolidine-5-carboxylic acid t-butyl ester for ()- (2R, 3R, 5R, 1'S)-1-t-butoxycarbonyl-2-oxiranyl-3-t-butyidiphenylsilyloxy methyl- pyrrolidine-5-carboxylic acid t-butyl ester.

190C (~)-(2R,3R,5R,1'R,2'R0-1-t-Butoxycarbonyl-2-(1-acetamido-1-( 2,- dimethyl-1, 3-dioxolan-4-yl)) methyl-3-formpyrrolidine-5-carboxylic Acid t-Butyl Ester The title compound was prepared according to the method described in Example 123H substituting (~)-(2R,3R,5R,1'R,2'R)-1-t-butoxycarbonyl-2-(1- acetamido-1- (2, 2-dimethyl-1, 3-dioxolan-4-yl)) methyl-3-hydroxymethyl-pyrrolidine- 5-carboxylic acid t-butyl ester for ()- (2R, 3R, 5R, 1'S)-1-t-butoxycarbonyl-2- oxiranyl-3-hydroxymethyl-pyrrolidine-5-carboxylic acid t-butyl ester.

190D (~)-(2R,3R,5R,1'R,2'R)-1-t-Butoxycarbonyl-2-(1-acetamido-1-( 2,2- dimethyl-1,3-dioxolan-4-yl))methyl-3-(cis-propen-1-yl))-pyrr olidine-5-carboxylic Acidt-Butyl Ester The title compound was prepared according to the method described in Example 35A substituting ()- (2R, 3R, 5R, 1'R, 2'R)-1-t butoxycarbonyl-2- (1- acetamido-1- (2, 2-dimethyl-1, 3-dioxolan-4-yl)) methyl-3-formyl-pyrrolidine-5- carboxylic acid t-butyl ester in place of ()- (2R, 3R, 5R, 1'S)-1-t-butoxycarbonyl-2- (1-acetamido-3-methyl) butyl-3-formyl-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 42 mg, 61%).

1H NMR (CDCl3): # 7.88 (d, 1H), 5.52 (m, 1H), 5.34 (m, 1H), 4.33 (m, 1H), 4.21 (m, 1H), 3.96 (m, 2H), 3.83 (m, 1H), 3.60 (m, 1H), 3.40 (m, 1H), 2.53 (m, 1H), 1.98 (s, 3H), 1.66 (dd, 3H), 1.46 (s, 9H), 1.44 (s, 9H), 1.41 (s, 3H), 1.33 (s, 3H).

MS: (M+H) +=483 190E (~)-(2R, 3S. 5R. 1'R, 2'R)-2-(1-Acetamido-2,3-dihydroxy)propyl-3-(cis- propen-1-yl)-pvrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41C, substituting ()- (2R, 3S, 5R, 1'R, 2'R)-1-t butoxycarbonyl-2- (1- acetamido-1-(2, 2-dimethyl-1, 3-dioxolan4-yl)) methyl-3-(cis-propen-1-yl))- pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t- butoxycarbonyl-2- (1-acetamido-2-hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5- carboxylic acid t-butyl ester. rH NMR (DMSO-d6) : # 7.98 (d, J=9Hz, 1H), 5.48 (m, 1H), 5.29 (m, 1H), 4.60 (m, 1H), 4.30 (m, 1H), 4.12 (m, 1H), 3.76 (m, 1H), 3.52 (m, 1H), 3.46 (m, 1 H), 3.32 (m, 1 H), 3.18 (m, 1 H), 2.40 (m, 1 H), 1.84 (s, 3H), 1.60 (dd, 3H).

MS: (M+H) +=287 Example 193 (+)-(2R. 3S. 5R. 1'R, 2'S)-2-1-Acetamido-2-ethoxv)Dentv)-3-fc/s-Dropen-1-v))- pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 193A (~)-(2R,3R,5R,1'R,2'S)-1-t-Butoxycarbonyl-2-(1-acetamido-2- ethoxy)penty)-3-(c/s-proDen-1-v))-pvrro)idine-5-carboxv)ic Acid t-Buyl Ester The title compound was prepared according to the method described in Example 88A, substituting ethyl iodide for methyl iodide (yield : 3.6 mg, 28%).

MS: (M+H) += 483, (M+Na)+= 505, (M-H)-= 481.

193B (~)- (2R,3S,5R,1'R,2'S)-2-(1-Acetamido-2-ethoxy)pentyl-3-(cis-pro pen-1- Ipyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41C, substituting (~)-(2R,3S, 5R, 1'R, 2'S0-1-t-butoxycarbonyl-2-(1- acetamido-2-ethoxy) pentyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t butoxycarbonyl-2- (1-acetamido-2- hydroxy)butyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester. ester (yield : 3.2 mg, 100%).

H NMR (DMSO-d6) 8 7.92 (d, J= 9.2Hz, 1H), 5.47 (m, 1H), 5.25 (m, 1H), 4.25 (m, 2H), 3.70 (m, 1H), 3.52 (m, 1H), 3.33 (m, 2H), 3.18 (m, 1H), 2.39 (m, 1H), 1.85 (s, 3H), 1.66 (m, 1H), 1.61 (dd, J= 6.7,1.8Hz, 3H), 1.56 (m, 1H), 1.37 (m, 1H), 1.28 (m, 2H), 1.13 (m, 3H), 0.86 (t, J= 7.3Hz, 3H).

MS: (M+H)+= 327, (M+Na)"= 349, (M-H)-= 325.

Example 194 (~)-(2R,3S,5R 1'R. 2'R)-2-1-Acetamido-2-ethoxv)Dentvt-3-fc/s-DroDen-1-v))- nvrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 194A (~)-(2R,3R,5R,1'R,2')-1-t-Butoxycarbonyl-2-(1-acetamido-2- <BR> <BR> <BR> ethoxy)pentyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic Acid t-Butyl Ester The title compound is prepared according to the method described in Example 88A, substituting ethyl iodide for methyl iodide.

194B (~)-(2R,3R,5R,1'R,2'R)-2-(1-Acetamido-2-ethoxy)pentyl-3-(cis -propen-1- yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound is prepared according to the method described in Example 41 C, substituting ()- (2R, 3S, 5R, 1'R, 2'R)-1-t-butoxycarbonyl-2-(1- acetamido-2-ethoxy) pentyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2- (1-acetamido-2- hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester.

Example 195 (t)- (2R, 3S. 5R, 1'S)-1-Acetamido-2-hydroxv) ethyl-3-vinyl-pyrrolidine-5- carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41 C, substituting ()- (2R, 3S, 5R, 1'S)-1-t-butoxycarbonyl-2-(1-acetamido- 2-hydroxy) ethyl-3-vinyl-pyrrolidine-5-carboxylic. acid t-butyl ester in place of (~)- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1-acetamido-2-hydroxy)butyl-3-(ci s- propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield: 19.9 mg, 100%).

'H NMR (DMSO-d6) 8 7.80 (d, J= 8.8Hz, 1 H), 5.76 (m, 1H), 5.23 (d, J= 17. 1Hz, 1H), 5.15 (m, 1H), 4.31 (m, 1H), 4.03 (m, 1H), 3.62 (m, 1H), 3.53 (m, 2H), 2.79 (m, 1 H), 2.42 (m, 1 H), 1.90 (s, 3H), 1.85 (m, 1 H).

MS: (M+H)+= 243, (M+Na) += 265, (M-H)-= 241.

Example 196 (~)-(2R,3S, 5R. 1'R. 2'S)-2- (1-Acetamido-2-hvdroxy-3-dimethylphosphonyl) propvl- 3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 196A (i)-(2R. 3S. 5R. 1'R. 2'S) and (i)-(2R. 3S. 5R. 1'R, 2'R)-1-t-Butoxycarbonyl-2-<BR> <BR> <BR> (1-acetamido-2-hydroxy-3-dimethylphosphonvl) propyl-3-Lis-propen-1-yl)- pyrrolidine-5-carboxylic Acid t-Butyl Ester.

()- (2R, 3S, 5R, 1'R)-1-t-Butoxycarbonyl 2- (1-acetamido-1-formyl) methyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (78 mg, 0.19 mmol) in THF (5 mL) was added dropwise to a solution of dimethylphosphonylmethyl lithium (3M) (0.32 mL, 0.95 mmol) in THF (20 mL) at-78°C and reacted for 40 minutes. The reaction was quenched with water (10 mL) and saturated aqueous ammonium chloride (10 mL) followed by extraction using dichloromethane (2 x 50 mL). The organic layer was dried over magnesium sulfate, filtered and concentrated in vacuo. The residue was purified by column chromatography on silica gel using 5-10% methanol in dichloromethane to provide the title compounds ()- (2R, 3S, 5R, 1'R, 2'R) isomer (yield : 27 mg, 27%) and (~)- (2R, 3S, 5R, 1'R, 2'S) isomer (yield : 5.5 mg, 6%).

()- (2R, 3S, 5R, 1'R, 2'R) = 1H NMR (CDCl3) # 5. 98 (m, 1H), 5.58 (m, 1H), 5.35 (m, 1H), 4.94 (m, 1H), 4.14 (m, 2H), 3.74 (m, 8H), 3.06 (m, 1H), 2.64 (m, 1H), 2.03 (s, 3H), 1.95 (m, 1H), 1.83 (m, 3H), 1.53 (s, 9H), 1.46 (s, 9H) MS: (M+H)+=535, (M-H)- =533 ()- (2R, 3S, 5R, 1'R, 2'S) MS: (M+H) +=535, (M-H)-=533 196B (~)-(2R,3R,5R,1'R,2'S)-2-(1-Acetamido-2-hydroxy-3- dimethylphosphonyl)propyl-3-(cis-propen-1-yl)-pyrrolidine-5- carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41C, substituting ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2- (1- <BR> <BR> <BR> <BR> acetamido-2-hydroxy-dimethylphosphonyl) propyl-3- (cis-propen-1-yl)-pyrrolidine- 5-carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)- 1-t- <BR> <BR> <BR> <BR> butoxycarbonyl-2- (1-acetamido-2-hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5- carboxylic acid t-butyl ester (yield : 3 mg, 96%).

1H) NMR (DMSO-d6) 8 7.98 (d, J=9.2 HZ, 1H), 5.48 (M, 1H), 5.28 (m, 1H), 4.36 (m, 1H), 4.30 (m, 1H), 4.08 (m, 2H), 3.70 (m, 2H), 3.60 (m, 6H), 3.18 (m, 1H), 2.40 (m, 1H), 2.05 (m, 1H), 1.85 (s, 3H), 1.60 (dd, J=6.2,1.2 HZ, 3H) MS: (M+H) +=379, (M-H)'=377 Example 197 (~)-(2R,3R,5R,1'R,2'R)-2-(1-Acetamido-2-hydroxy-3-dimethylph osphonyl)propy- 3- (cis-propen-1-y-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41C, substituting (~)-(2R,3S, 5R, 1'R, 2'R)-1-t-butoxycarbonyl-2-(1- <BR> <BR> <BR> acetamido-2-hydroxy-dimethylphosphonyl) propyl-3- (cis-propen-1-yl)-pyrrolidine- 5-carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t- butoxycarbonyl-2-(1-acetamido-2-hydroxy)butyl-3-(cis-propen- 1-yl)-pyrrolidine-5- carboxylic acid t-butyl ester (yield : 13 mg, 96%).

'H NMR (DMSO-d6) 8 7.72 (d, J=9.2 HZ, 1H), 5.48 (m, 1 H), 5.24 (m, 1H), 4.44 (m, 1 H), 4.15 (m, 2H), 3.62 (m, 7H), 3.54 (m, 1 H), 3.15 (m, 1 H), 2.40 (m, 1 H), 1.95 (m, 1H), 1.82 (s, 3H), 1.72 (m, 1H), 1.54 (dd, J=6.7,1.2 HZ, 3H) MS: (M+H)+= 379, (M-H)- = 377 Example 198 (~)-(2R,3R,5R,1'R,2'S)-2-(1-Acetamido-3-hydroxy)propyl-3-(ci s-propen-1-yl)- nvrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 198A (i)-(2R. 3S. 5R. 1'S)-1-t-Butoxycarbonyl-2-(1-acetamido-1-(cis and tans-2- methoxyvinyl))methyl-3-(cis-propen-1-yl)-pyrrolidine-5-carbo xylic Acid t Butvl Ester ()- (2R, 3S, 5R, 1'R)-1-t-Butoxycarbonyl-2- (1-acetamido-1-formyl) methyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (113 mg, 0.28 mmol) was added to a solution of (methoxymethyl) triphenylphosphonium bromide (240 mg, 0.70 mmol) and potassium t-butoxide (0.56 mL, 0.56 mmol, 1M in THF) in toluene (3 mL) at 0°C for 15 minutes. The reaction was quenched with saturated aqueous ammonium chloride (3 mL) followed by extraction using dichloromethane (2 X 3 mL). The organic layer was dried over magnesium sulfate, filtered and concentrated in vacuo. The residue was purified by column chromatography on silica gel using 1/4 : ethyl acetate/hexane to provide the title compounds 'H NMR (CDC13) s 8.65 (br d, 1 H) 6.01 (d, J=5.7Hz, 1 H), 5.40 (m, 3H), 5.11 (br t, 1 H), 4.15 (m, 2H), 3.72 (m, 1H) 3.61 (s, 3H), 3.00 (m, 1 H), 2.42 (m, 1 H), 1.94 (s,-3H), 1.64 (dd, J=1.4, 5:0Hz, 3H), 1.45 (m, 9H), 1.25 (m, 9H) MS : (M+H)+ = 439.

198 B (~)-(2R,3R,5R,1'R,2'S)-1-t-Butoxycarbonyl-2-(1-acetamido-2-f ormyl)ethyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic Acid t-Butyl Ester ()- (2R, 3S, 5R, 1'S)-1-t-Butoxycarbonyl-2- (1-acetamido-2- (cis and trans-2- methoxyvinyl)) methyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (21 mg, 0.048 mmol) was reacted with LiBr (37 mg, 0.43 mmol) and AG50W-X2 ion exchange resin in CH3CN (2 mL) and water (0.1 mL) at room temperature for 45 minutes. The reaction was filtered and quenched with saturated aqueous sodium bicarbonate (1 mL) followed by extraction using dichloromethane (2 X 1 mL). The organic layer was dried over magnesium sulfate, filtered and concentrated in vacuo. The residue was purified by column chromatography on silica gel using 1/4: ethyl acetate/hexane to provide the title compound.

'H NMR (CDCI3) ä 9. 70 (dd, J=1. 3, 2.4Hz, 1H), 8.11 (d, J=7.8Hz, 1H), 5.54 (m, 1H), 5.41 (t, J=5.8Hz, 1 H), 4.52 (m, 1H), 4.13 (dd, J=4.4,4.8Hz, 1H), 3.75 (dd, J=2.7,3.1 Hz, 1 H), 2.86 (m, 1H), 2.47 (m, 3H), 1.99 (s, 3H), 1.63 (dd, J=1.6, 5. 1Hz, 3H), 1.46 (s, 9H), 1.45 (m, 1H), 1.44 (s, 9H) MS: (M+H)+ = 425 ; (M-H)-= 423.

198C (t)- (2R, 3S. 5R. 1'S)-1-t-Butoxycarbonyl-2- 1-acetamido-3-hydroxy) propyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic Acid t-Butyl Ester ()- (2R, 3S, 5R, 1'S)-1-t-Butoxycarbonyl-2- (1-acetamido-2-formyl) ethyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (9 mg, 0.02 mmol) was reacted with sodium borohydride (1 mg, 0.02 mmol) in methanol (0.1 mL) at room temperature for 20 minutes. The reaction was quenched with saturated aqueous ammonium chloride (1 mL) followed by extraction using dichloromethane (2 X 1 mL). The organic layer was dried over magnesium sulfate, filtered and concentrated in vacuo. The residue was purified by column chromatography on silica gel using 1/4: ethyl acetate/hexane to provide the title compound. rH NMR (CDCl3) 8 8.45 (d, J=7.5Hz, 1 H), 5.55 (m, 1 H), 5.34 (t, J=7.8Hz, 1H), 4.20 (dd, J=3.0,5.4Hz, 2H), 3.71 (d, J=6.1Hz, 1H), 3.62 (m, 1H), 3.50 (t, J=9. 1Hz, 1H), 2.92 (m, 1H), 2.41 (m, 1H), 2.04 (s, 3H), 1.66 (dd, J=2.0,5. 1Hz, 3H), 1.62 (m, 1H), 1.47 (s, 9H), 1.45 (m, 1H), 1.43 (s, 9H), 1.22 (m, 2H) MS: (M+H) + = 427 ; (M-H)-= 425.

198D ()- (2R, 3S. 5R. 1'S)-2-(1-Acetamido-3-hydroxy)propyl-3-(cis-propen-1-yl)- pyrrolidine-5-carboxvlic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41C, substituting ()- (2R, 3S, 5R, 1'S)-1-t-butoxycarbonyl-2-(1-acetamido- 2-hydroxy) propyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)- 1-t-butoxycarbonyl-2-(1-acetamido-2- hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester. ester (yield : 4.6 mg, 100%).

'H NMR (DMSO-d6) 8 9.25 (br s, 1H), 8.13 (d, J=7.3Hz, 1H), 5.52 (m, 1H), 5.28 (brt, 1H), 4.32 (brt, 1H), 4.22 (m, 1H), 3.49 (m, 4H), 3.18 (m, 1H), 2.40 (m, 1H), 1.90 (s, 3H), 1.73 (m, 1H), 1.63 (dd, J=1.8,5.5Hz, 3H), 1.57 (m, 1H) MS: (M-H)-= 269; (M+H) + = 271.

Example 199 (~)-(2R,3R,5R,1'R,2'S)-2-(1-Acetamido-3-hydroxy)pentyl-3-(ci s-propen-1-yl)- pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 199A(~)-(2R,3R,5R,1'R,2'S) and (~)-(2R,3R,5R,1'R,2'R)-1-t-Butoxycarbonyl-2- <BR> <BR> <BR> (1-acetamido-3-hydroxy) ethyl-3- (cis-propen-1-yl-pyrrolidine-5-carboxylic Acid t-<BR> <BR> <BR> <BR> <BR> Butyl Ester ()- (2R, 3S, 5R, 1'S)-1-t-Butoxycarbonyl-2- (1-acetamido-2-formyl) ethyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (26 mg, 0.061 mmol) was reacted with ethylmagnesium bromide (3.0 M) (0.122 mL, 0.367 mmol) in THF (4 mL) at room temperature for 30 minutes. The reaction was quenched with saturated aqueous ammonium chloride (10 mL) and water (10 mL) followed by extraction using ethyl acetate (3 X 25 mL). The organic layer was dried over magnesium sulfate, filtered and concentrated in vacuo. The residue was purified by column chromatography on silica gel using 1/1 ethyl acetate/hexane followed by 2/1 ethyl acetate/hexane to provide the title compounds ()- (2R, 3S, 5R, 1'S, 2'S) (yield : 6.7 mg, 24%) and (~)-(2R,3S, 5R, 1'S, 2'R) (yield: 6.8 mg, 24%).

()- (2R, 3S, 5R, 1'S, 2'S) MS : (M+H) +=455, (M+Na) +=477, (M-H)-=453.

()- (2R, 3S, 5R, 1'S, 2'R) MS: (M+H)+=455, (M+Na)+=477, (M-H)-=453.

199B (~)-(2R,3R,5R,1'R,2'S)-2-(1-Acetamido-3-hydroxy)pentyl-3-(ci s-propen-1- yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41C, substituting ()- (2R, 3S, 5R, 1'S, 3'S)-1-t-butoxycarbonyl-2-(1- acetamido-3-hydroxy) pentyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t- butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1-acetamido- 2-hydroxy) butyl-3-(cis-propen-1-yl)pyrrolidine-5-carboxylic acid t-butyl ester. ester (yield : 6.2 mg, 100%).

'H NMR (DMSO-d6) # 9.20 (bs, 1H), 8.18 (d, J=7.3 Hz, 1H), 5.51 (m, 1 H), 5.27 (m, 1H), 4.30 (m, 1H), 4.25 (m, 1H), 3.58 (m, 1H), 3.41 (m, 1H), 3.18 (m, 1H), 2.39 (m, 1H), 1.90 (s, 3H), 1.75 (m, 1H), 1.64 (dd, J=7.5,1.5Hz, 3H), 1.51 (M, 1H), 1.38 (m, 1H), 1.32 (m, 1H), 0.83 (t, J=7.3Hz, 3H).

MS : (M+H) + = 299, (M+Na) + = 321, (M-H)- = 297, (2M-H)-=595.

Example 200 (~)-(2R,3R,5R,1'R,2'R)-2-(1-Acetamido-3-hydroxy)pentyl-3-(ci s-propen-1-yl)- pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41C, substituting ()- (2R, 3S, 5R, 1'S, 3'R)-1-t-butoxycarbonyl-2-(1- acetamido-3-hydroxy)pentyl-3-(cis-propen-1-yl)-pyrrolidine-5 -carboxylic acid t- butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1-acetamido- 2-hydroxy)butyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester. ester (yield : 6.5 mg, 100%).

'H NMR (DMSO-d6) 8 9.25 (bs, 1H), 8.15 (d, J=7.3 Hz, 1H), 5.52 (m, 1H), 5.27 (m, 1H), 4.31 (m, 2H), 3.52 (m, 1H), 3.36 (m, 1H), 3.19 (quint., J=8.5Hz, 1H), 2.38 (m, 1H), 1.92 (s, 3H), 1.75 (m, 1H), 1.64 (dd, J=7.3,1.5Hz, 3H), 1.48 (m, 1H), 1.33 (m, 2H), 0.85 (t, J=7.3Hz, 3H).

MS : (M+H) + = 299, (M+Na)+ = 321, (M-H)- = 297, (2M-H)-=595.

Example 201 (~)-(2R,3R,5R,1'R,2'R)-2-(1-Acetamido-2-oxo-3,3-difluoro-3-v inyl)propyl-3-(cis- propen-1-yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41 C, substituting ()- (2R, 3S, 5R, 1'R)-1-t-butoxycarbonyl-2- (1- Acetamido-2-oxo-3,3-difluoro-3-vinyl)propyl-3-(cis-propen-1- yl)-pyrrolidine-5- carboxylic Acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t- <BR> <BR> <BR> butoxycarbonyl-2- (1-acetamido-2-hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5- carboxylic acid t-butyl ester (yield : 0.0050 g, 100%).

1H NMR (DMSO-d6) s 8.67 (d, J=8.5Hz, 1 H), 6.1-5.95 (m, 1 H), 5.78 (dd, J=17.1, 2.4Hz, 1H), 5.71 (d, 11.0Hz, 1H), 5.45 (m, 1H), 5.12 (m, 1H), 4.94 (t, J=9.2Hz, 1H), 4.51 (dd, J=12.2, 6.1Hz, 1H), 3.98 (m, 1H), 3.24 (m, 1H), 2.32 (m, 1H), 1.73 (s, 3H), 1.66 (q, J=11 9Hz. 1H), 1.57 (dd, J=6.7,1.8Hz, 3H).

MS: (M+H) + = 331, (M+H2O)+=349, (M+Na)+ = 353, (M-H)- = 329, (2M-H)- =659.

Example 202 202A (i)-(2R. 3R. 5R. 1'R, 2'S) and ()- (2R, 3R. 5R. 1'R, 2'R)-1-t-Butoxycarbonyl-2- (1-acetamido-2-hydroxy)butyl-3-t-butyldiphenylsilyloxymethyl -pyrrolidine-5- carboxylic Acid t-Butyl Ester The title compounds were prepared according to the method described in Example 41 B substituting (~)-(2R, 3R, 5R, 1'R)-1-t butoxycarbonyl-2- (1-acetamido- <BR> <BR> <BR> <BR> 2-formyl) ethyl-3-t-butyidiphenylsilyloxymethyl-pyrrolidine-5-carboxyl ic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R)-1-t-butoxycarbonyl-2- (1-acetamido-2- formyl) ethyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester to provide ()- (2R, 3R, 5R, 1'R, 2'S) isomer (yield : 370 mg, 17%) (~)- (2R, 3R, 5R, 1'R, 2'R) isomer (yield : 1.2 g, 55%).

()- (2R, 3R, 5R, 1'R, 2'S) 1 H NMR (d6-DMSO) 8 7. 4-7.65 (m, 10H), 4.47 (d, 1H), 4.32 (m, 1H), 3.87 (m, 2H), 3.68 (m, 1H), 3.55 (m, 1H), 3.25 (m, 1H), 2.7 (m, 1 H), 2.45 (m, 1 H), 2.0 (m, 1 H), 1.83 (d, 3H), 1.28-1.4 (m, 18H), 0.95 (d, 9H), 0.83 (dt, 3H) MS: (M-H)-= 667, (M+35) + = 703; (M+H) + = 669, (M+Na) + = 691 ()- (2R, 3R, 5R, 1'R, 2'R) 1H NMR(d6-DMSO) # 7.4-7.65 (m, 10H), 4.40 (dd, 1H), 4. 12-4. 32 (m, 1H), 3.82-3. 96. (m, IH),.-3. 66. (m,-2H), 3.52 (t, 1H), 2.6-2.8 (m, 1H), 2.45 (m, 1H), 1.76-2.0 (m, 1H), 1.87 (d, 3H), 1.25-1.4 (m, 18H), 0.95 (d, 9H), 0.83 (dt, 3H).

MS: (M-H)-= 667, (M+35) + = 703; (M+H) + = 669, (M+Na) + = 691 202B t)-j2R, 3R. 5R. 1'R. 2'S)-1-t-Butoxvcarbonvl-2- (1-acetamido-2- methoxymethyloxy)butyl-3-t-butyldiphenylsilyloxymethyl-pyrro lidine-5-carboxylic Acid t-Butyl Ester ()- (2R, 3R, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2- (1-acetamido-2- hydroxy) butyl-3-t-butyidiphenylsilyloxymethyl-pyrrolid ine-5-carboxylic acid t-butyl ester (0.58 g, 0.87 mmole) was reacted with methoxymethyl chloride (1.15 mL, 10.07 mmole) and diisopropylethylamine (3.5 mL, 20.1 mmole) in dichloromethane (1 mL) at room temperature for 5 hours. The reaction was quenched with saturated NH4CI (100 mL) and diluted with ethyl acetate (200 mL).

The organic layer was washed with water, and brine, dried over MgS04, filtered and concentrated in vacuo. The residue was purified by chromatography on silica gel using 5% methanol/methylene chloride to provide the title compound (yield : 0.64 g, 98%).

1H NMR(d6-DMSO) # 7. 4-7.65 (m, 10H), 4.70 (s, 1 H), 4.62 (s, 1 H), 4.35- 4.55 (m, 2H), 3.75-3.95 (m, 2H), 3.68 (m, 1 H), 3.55 (m, 1H), 3.25 (m, 1H), 3.24 (s, 3H), 2. 55 (m, 1H), 2. 45 (m, 1H), 2.0 (m, 1 H), 1.85 (s, 3H), 1.28-1.4 (m, 18H), 0.99 (d, 9H), 0.8 (dt,-3H) MS: (M-H)-= 755, (M+35) + = 791; (M+H) + = 757, (M+Na) + = 779 202C (~)-(2R,3R,5R,1'R,2'S)-1-t-Butoxycarbonyl-2-(1-acetamido-2- methoxymethyloxy)butyl-3-hydroxymethyl-pyrrolidine-5-carboxy lic Acid t-Butyl Ester The title compound was prepared according to the method described in Example 123G substituting ()- (2R, 3R, 5R, 1'R, 2'S)-1-t-butoxywarbonyl-2-(1- <BR> <BR> <BR> <BR> acetamido-2-methoxymethyloxy) butyl-3-t-butyidiphenylsilyloxymethyl-pyrrolidine- 5-carboxylic acid t-butyl ester in place of ()- (2R, 3R, 5R, 1'S)-1-t-butoxycarbonyl- 2-oxiranyl-3-t-butyidiphenylsilyloxymethyl-pyrrolidine-5-car boxylic acid t-butyl ester (yield : 0.416 g, 95%).

1 H NMR (d6-DMSO) 5 7.45 (t, 1 H), 4.62-4.74 (m, 3H), 4.48 (m, 1 H), 3.85 (m, 2H), 3.55-3.6 (m, 2H), 3.45 (t, 1H), 3.2-3.4 (m, 2H), 3.25 (d, 3H), 2.4 (m, 2H), 1.82 (d, 3H), 1.58 (m, 3H), 1.32-1.45 (m, 18H), 0.82 (dt, 3H).

MS: (M-H)-= 517, (M+35) + = 553; (M+H) + = 519, (M+Na) + = 541 202D (~)-(2R,3R,5R,1'R,2'S)-1-t-Butoxycarbonyl-2-(1-acetamido-2- methoxvmethv) butyl-3-formyl-pyrrolidine-5-carboxylic Acid t-Butyl Ester The title compound was prepared according to the method described in Example 123H substituting ()- (2R, 3R, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- <BR> <BR> <BR> acetamido-2-methoxymethyloxy) butyl-3-hyd roxymethyl-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3R, 5R, 1'S)-1-t-butoxycarbony !-2-oxiranyl-3- hydroxymethyl-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 0.335 g, 80.8%).

1 H NMR (d6-DMSO) 8 9.55 (d, 1 H), 7.48 (m, 1 H), 4.55-4.72 (m, 4H) 3.9 (d, 1H), 3.6 (m, 2H), 3.45 (m, 3H), 3.32 (s, 3H), 3.05 (t, 1H), 2.25-2.45 (m, 4H), 1.83 (s, 3H), 1.58 (m, 3H), 1.30-1.45 (m, 18H), 0.86 (dt, 3H).

MS: (M-H)-= 515, (M+35) += 551; (M+H) + = 517 202E (t)- (2R, 3R. 5R, 1'R. 2'S. 1"RS)-1-t-Butoxycarbonvl-2- (1-acetamido-2-<BR> <BR> <BR> methoxvmethv) buty1-hydroxv-2-propyn-1yl)-pyrrolidine-5-carboxylicAcid t-ButylEster The title compound was prepared according to the method described in Example 38A substituting ()- (2R, 3R, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- acetamido-2-methoxymethyloxy) butyl-3-formyl-pyrrolidine-5-carboxylic acid t- butyl ester in place of ()- (2R, 3R, 5R, 1'S)-1-t butoxycarbonyl-2- (1-acetamido-3- methyl) butyl-3-formyl-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 0.27 g, 83%).

MS: (M-H)-= 541, (M+35) + = 577; (M+H) + = 543, (M+Na) + = 565 202F (i)-(2R, 3R, 5R. 1'R. 2'S)-1-t-Butoxycarbonyl-2- (1-acetamido-2- methoxymethyloxy)butyl-3-(1-oxo-2-propyn-1-yl)-pyrrolidine-5 -carboxylic Acid t- ButylEster The title compound was prepared according to the method described in Example 38B substituting ()- (2R, 3R, 5R, 1'R, 2'S, 1"RS)-1-t-butoxycarbonyl-2-(1- acetamido-2-methoxymethyloxy) butyl-3- ( 1-hyd roxy-2-propyn-1-yl)-pyrrolidine-5- carboxylic acid t-butyl ester in place of ()- (2R, 3R, 5R, 1'S, 1"RS)-1-t- butoxycarbonyl-2- (l-acetamido-3-methyl) butyl-3- (l-hydroxy-2-propyn-1-yl)- pyrrolidine-5-carboxylic acid t-butyl ester (yield : 0.2 g, 74%).

1 H NMR (d6-DMSO) 57. 49 (br d, 1 H), 5.0 (d, 1 H), 4.7 (br s, 1 H), 4.55-4.7 (m, 3H), 3.88 (br d, 1 H), 3.5-3.7 (m, 2H), 3.43 (t, 2H), 3.2-3.4 (m, 2H), 3.24 (s, 3H), 2.4-2.7 (m, 2H), 1.84 (s, 3H), 1.5-1.7 (m, 2H), 1.30-1.45 (m, 18H), 0.86 (dt, 3H) MS: (M-H)-= 539, (M+35) + = 575; (M+H) + = 541, (M+Na) + = 563 202G (~)-(2R,3R,5R,1'R,2'S)-1-t-Butoxycarbonyl-2-(1-acetamido-2- methoxymethyloxy)butyl-3-(pyrazol-3-yl)-pyrrolidine-5-carbox ylic Acid t-Butyl Ester The title compound was prepared according to the method described in Example 38C substituting ()- (2R, 3R, 5R, 1'R, 2'S)-1-t butoxycarbonyl-2- (1- acetamido-2-methoxymethyloxy) butyl-3-(1-oxo-2-propyn-1-yl)-pyrrolidine-5- carboxylic acid t-butyl ester in place of ()- (2R, 3R, 5R, 1'S)-1-t-butoxycarbonyl-2- (1-acetamido-3-methyl) butyl-3- (1-oxo-2-propyn-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 180 mg, 87%).

1H NMR (d6-DMSO) 5 7.57 (br t, 2H), 6.1 (d, 1H), 4.50-4.7 (m, 4H) 3.95 (m, 1 H), 3.4-3.6 (m, 3H), 3.3-3.4 (m, 3H), 3.22 (d, 3H), 2.55-2.65 (m, 1 H), 2.2 (m, 1H), 1.85 (s, 3H), 1.5-1.7 (m, 2H), 1.15-1.45 (m, 18H), 0.86 (dt, 3H).

MS: (M-H)-= 553, (M+35) + = 589; (M+H) + = 553, (M+Na)+ = 577 202H (~)-(2R,3R, 5R. 1'R. 2'S)-2-(1-Acetamido-2-hydroxy)butyl-3-(pyrazol-3-yl)- pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 1K, substituting ()- (2R, 3R, 5R, 1'R, 2'S)-1-t butoxycarbonyl-2- (1- acetamido-2-methoxymethyloxy) butyl-3- (pyrazol-3-yl)-pyrrolidine-5-carboxylic acid t-butyl ester in place ()- (2R, 3R, 5R, 1'S)-2-(1-acetamido-3-ethyl)pentyl-3- methoxymethyl-pyrrolidine-5-carboxylic acid t-butyl ester. Chromatography on silica gel with 2-propanol : acetic acid: ethyl acetate: water 1: 1: 3: 1 followed by the addition of 0. 1% trifluoroacetic acid gave the title compound (yield : 15 mg, 55%).

1 H NMR (d6-DMSO) 6 7.95 (d, 1 H), 7.65 (br s, 1 H), 6, 18 (d, 1 H), 4.37 (m, 1H), 4.23 (m, 1H), 4.38 (m, 1H), 4.56 (m, 1H), 2.63 (m, 1H), 2.10 (m, 1H), 1.78 (s, 3H), 1.50 (m, 1 H), 1.25 (m, 1 H), 0.83 (t, J=7.46 Hz, 3H).

MS: (M-H3-=-309,-(M+35) + = 345 ; (M+H) + = 311, (M+Na) + = 333 Example 203 (~)-(2R,3R,5R,1'R,2'R)-2-(1-Acetamido-2-hydroxy)butyl-3-(pyr azol-3-yl)- pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 203B (~)-(2R,3R,5R,1'R,2'R)-1-t-Butoxycarbonyl-2-(1-acetamido-2- methoxymethyloxy)butyl-3-t-butyldiphenylsilyloxymethyl-pyrro lidine-5-carboxylic Acidt-Butyl Ester The title compounds were prepared according to the method described in Example 202B substituting ()- (2R, 3R, 5R, 1'R, 2'R)-1-t-butoxycarbonyl-2-(1- acetamido-2-hydroxy) butyl-3-t-butyldiphenylsilyloxymethyl-pyrrolidine-5- carboxylic acid t-butyl ester in place of ()- (2R, 3R, 5R, 1'R, 2'S)-1-t- <BR> <BR> <BR> <BR> butoxycarbonyl-2- (1-acetamido-2-hydroxy) butyl-3-f-butyidiphenylsilyloxymethyl- pyrrolidine-5-carboxylic acid t-butyl ester (yield : 0.217 g, 96%).

1 H NMR (d6-DMSO) 87. 4-7.65 (m, 10H), 4.70 (s, 1H), 4.62 (s, 1H), 4.35- 4.55 (m, 2H), 3.75-3.95 (m, 2H), 3.68 (m, 1H), 3.55 (m, 1H), 3.25 (m, 1H), 3.24 (s, 3H), 2.55 (m, 1H), 2.45 (m, 1H), 2.0 (m, 1H), 1.85 (s, 3H), 1.28-1.4 (m, 18H), 0.99 (d, 9H), 0.8 (dt, 3H).

MS: (M-H)- = 755, (M+35) +-= 791; (M+H) + = 757, (M+Na) + = 779 203 C (~)-(2R,3R,5R,1'R,2'R)-1-t-Butoxycarbonyl-2-(1-acetamido-2- methoxymethyloxy)butyl-3-hydroxymethyl-pyrrolidine-5-carboxy lic Acid t-Butyl Ester The title compound was prepared according to the method described in Example 123G substituting ()- (2R, 3R, 5R, 1'R, 2'R)-1-t-butoxycarbonyl-2-(1- <BR> <BR> <BR> acetamido-2-methoxymethyloxy) butyl-3-t-butyidiphenylsilyloxymethyl-pyrrolidine- 5-carboxylic acid t-butyl ester in place of ()- (2R, 3R, 5R, 1'S)-1-t-butoxycarbonyl- 2-oxiranyl-3-t-butyidiphenylsilyloxymethyl-pyrrolidine-5-car boxylic acid t-butyl ester (yield : 0.124 g, 83%).

1 H NMR (d6-DMSO) 57. 42 (dd, 1 H), 4.62-4.8 (m, 3H), 4.48 (m, 1 H), 3.6- 3.85 (m, 3H), 3.35-3.6 (m, 4H), 3.25 (s, 3H), 2.25 (m, 1 H), 2.4 (m, 1 H), 2.28 (m, 1 H), 1.82 (s, 3H), 1.58 (m, 3H), 1.32-1.45 (m, 18H), 0.9 (dt, 3H).

MS : (M-H)-= 517, (M+35) + = 553; (M+H) + = 519, (M+Na)+ = 541 203D (~)-(2R,3R,5R,1'R,2'R)-1-t-Butoxycarbonyl-2-(1-acetamido-2- methoxymethvloxy butvl-3-formvl-pyrrolidine-5-carboxylic Acid t-Butvl Ester The title compound was prepared according to the method described in Example 123H substituting (i)-(2R, 3R, 5R, 1'R, 2'R)-1-t-butoxycarbonyl-2-(1- acetamido-2-methoxymethyloxy) butyl-3-hydroxymethyl-pyrrolidine-5-carboxylic acid t-butyl ester in place of (~)-(2R, 3R, 5R, 1'S)-1-t-butoxycarbonyl-2-oxiranyl-3- hydroxymethySpyrrolidine-5-carboxylic acid t-butyl ester (yield : 0.106 g, 86%).

1 H NMR (d6-DMSO) 89. 58 (d, 1 H), 7.58 (dd, 1 H), 4.6-4.72 (m, 3H), 4.48 (d, 1H), 3.88 (d, 1H), 3.4-3.65 (m, 5H), 3.24 (s, 3H), 3.15 (dd, 1H), 2.20-2.48 (m, 4H), 1.86 (s, 3H), 1.58 (m, 3H), 1.30-1.40 (m, 18H), 0.86 (t, 3H).

MS: (M-H)-= 515, (M+35) + = 551; (M+H) + = 517 203E (~)-(2R,3R,5R,1'R,2'R,1"RS)-1-t-Butoxycarbonyl-2-(1-acetamid o-2- <BR> <BR> <BR> <BR> methoxvmethvioxv) butyl-3-(1-hvd roxv-2-propyn-1-vl)-pvrrolidine-5-carboxYlic Acid t-But Ester The title compound was prepared according to the method described in Example 38A substituting ()- (2R, 3R, 5R, 1'R, 2'R)-1-t-butoxywarbonyl-2-(1- acetamido-2-methoxymethyloxy) butyl-3-formyl-pyrrolidine-5-carboxylic acid t- butyl ester in place of ()- (2R, 3R, 5R, 1'S, 1"RS)-1-t-butoxycarbonyl-2-(1- acetamido-3-methyl) butyl-3-formyl-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 32 mg, 76%).

MS : (M-H)- = 541, (M+35) + = 577; (M+H) + = 543, (M+Na)+ = 565 203F (~)-(2R,3R,5R, 1'R. 2'R)-1-t-Butoxvcarbonyl-2- (1-acetamido-2- <BR> <BR> <BR> methoxvmethv)oxv)butv!-3-M-oxo-2-propyn-l-y!)-pvrro!idine-5- carboxytic Acid t- ButylEster The title compound was prepared according to the method described in Example 38B substituting ()- (2R, 3R, 5R, 1'R, 2'R)-1-t-butoxycarbonyl-2-(1- acetamido-2-methoxymethyloxy)butyl-3- (1-hydroxy-2-propyn-1-yl)-pyrrolidine-5- carboxylic acid t-butyl ester in place of ()- (2R, 3R, 5R, 1'S, 1"RS)-1-t- butoxycarbonyl-2- (1-acetamido-3-methyl) butyl-3- ( 1-oxo-2-propyn-1-yl)- pyrrolidine-5-carboxylic acid t-butyl ester (yield : 25 mg, 78%).

1 H NMR (d6-DMSO) 8 7.49 (br d, 1 H), 5.0 (d, 1 H), 4.7 (br s, 1 H), 4.55-4.7 (m, 3H) 3.88 (br d, 1 H), 3.5-3.7 (m, 2H), 3.43 (t, 2H), 3.2-3.4 (m, 2H), 3.24 (s, 3H), 2.4-2.7 (m, 2H), 1.84 (s, 3H), 1.5-1.7 (m, 2H), 1.30-1.45 (m, 18H), 0.86 (dt, 3H).

MS: (M-H)-= 539, (M+35) + = 575; (M+H) + = 541, (M+Na) + = 563 203G (~)-(2R,3R,5R,1'R,2'R)-1-t-Butoxycarbonyl-2-(1-acetamido-2- methoxymethyloxy) butvl-3- (pvrazol-3-yl)-pyrrolidine-5-carboxylic Acid t-But Ester The title compound was prepared according to the method described in Example 38C substituting ()- (2R, 3R, 5R, 1'R, 2'R)-1-t-butoxycarbonyl-2- (1- acetamido-2-methoxymethyloxy) butyl-3-(1-oxo-2-propyn-1-yl)-pyrrolid ine-5- carboxylic acid t-butyl ester in place of ()- (2R, 3R, 5R, 1'S)-1-t-butoxycarbonyl-2- (1-acetamido-3-methyl) butyl-3- (1-oxo-2-propyn-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 18 mg, 72%).

1 H NMR (d6-DMSO) 8 7.57 (m, 2H), 6.1 (d, 1H), 4.40-4.7 (m, 4H) 3.93 (m, 1 H), 3.4-3.6 (m, 3H), 3.3-3.4 (m, 3H), 3.22 (d, 3H), 2.55-2.65 (m, 1H), 2.2 (m, 1H), 1.85 (s, 3H), 1.5-1.7 (m, 2H), 1.15-1.45 (m, 18H), 0.86 (m, 3H).

MS: (M-H)-= 553, (M+35) + = 589; (M+H) + = 553, (M+Na) + = 577 203H (~)-(2R,3R,5R,1'R,2'R)-2-(1-Acetamido-2-Hydroxy)butyl-3-(pyr azol-3-yl)- pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 15B, substituting ()- (2R, 3R, 5R, 1'R, 2'R)-1-t-butoxycarbonyl-2-(1- acetamido-2-methoxymethyloxy)butyl-3-(pyrazol-3-yl)-pyrrolid ine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3R, 5R, 1'S)-1-benzyl-2-(1-acetamido-3- ethyl)pentyl-3-(imidazol-2-yl)-pyrrolidine-5-carboxylic acid t-butylester.

Chromatography on silica gel with 2-propanol : acetic acid: ethyl acetate: water 1 : 1: 3: 1 followed by the addition of 0.1 % trifluoroacetic acid gave the title compound (yield : 4 mg, 45%). iHNMR (de-DMSO) 5 7. 65 (d, 1H), 7.64 (d, 1H), 6,16 (d, 1H), 4.37 (m, 1H), 4. 23 (m, 1H), 4.38 (m, 1H), 4.56 (m, 1H), 2.63 (m, 1H), 2.10 (m, 1H), 1.74 (s, 3H), 1.25-1.40 (m, 2H), 0.83 (t, J=7.46 Hz, 3H).

MS: (M-H)- = 309, (M+35) + = 345; (M+H) + = 311, (M+Na) + = 333 Example 204 ()- (2R. 3R. 5R)-2-Acetamidomethyl-3-methoxycarbonyl-pyrrolidine-5-carbox ylci Acid Hydrochloride.

204A (~)-(2R,3R,5R)-1-Benzyl-2-aminomethyl-3-t-btuyldimethylsilyl oxymethyl- pyrrolidine-5-carboxylic Acid t-ButylEster The title compound is prepared according to the method described in Example 1 F, substituting (~)-(2R,3R,5R)-1-benzyl-2-formyl-3-t- butyldimethylsilyloxymethyl-pyrrolidine-5-carboxylic acid t-butyl ester in place of (~)-(2R, 3R, 5R)-1-benzyl-2-(1-oxo-3-ethyl)pentyl-3-t-butyldimethylsilylo xymethyl- pyrrolidine-5-carboxylic acid t-butyl ester.

MS: (M+H) += 435 204B (~)-(2R,3R,5R)-1-Benzyl-2-acetamidomethyl-3-t- butyldimethylsilyloxymethyl-pyrrolidine-5-carboxylic Acid t-Butyl Ester The title-compound-is-prepared according to-the method described in Example 1G, substituting ()- (2R, 3R, 5R)-1-benzyl-2-aminomethyl-3-t- butyidimethylsilyloxymethyl-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3R, 5R, 1'R)- and (~)-(2R, 3R, 5R, 1'S)-1-benzyl-2-(1-amino-3-ethyl)pentyl-3- t-butyldimethylsilyloxymethyl-pyrrolidine-5-carboxylic acid t-butyl ester.

'H NMR (CDCb) : 6 7.2-7.35 (m, 5H), 6.14 (br, 1H), 3.86 (dd, J=18Hz, 13.5Hz, 2H), 3.67 (m, 1 H), 3.60 (m, 1 H), 3.49 (m, 1 H), 3.28 (m, 1 H), 3.06 (m, 1H), 2.19 (m, 2H), 1.95 (s, 3H), 1.45 (s, 9H), 0.91 (s, 9H), 0.07 (s, 6H).

MS: (M+H) += 477 204C ()- (2R, 3R. 5R)-l-Benzyl-2-acetamidomethyl-3-hydroxymethyl-pyrrolidine- 5-carboxylic Acid t-Butyl Este The title compound was prepared according to the method described in Example 1H, substituting ()- (2R, 3R, 5R)-1-benzyl-2-acetamidomethyl-3-t- butyldimethylsilyloxymethyl-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3R, 5R, 1'S)-1-benzyl-2- (1-acetamido-3-ethyl) pentyl-3-t- butyldimethylsilyloxymethyl-pyrrolidine-5-carboxylic acid t-butyl ester.

204D ()- (2R, 3R. 5R)-1-Benzyl-2-acetamidomethyl-3-formyl-pvrrolidine-5- carboxylic Acid t-Butyl Ester The title-compound was prepared according to the method described in Example 2A, substituting (~)-(2R,3R,5R)-1-Benzyl-2-acetamidomethyl-3- hydroxymethyl-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2 R, 3R, 5R, 1'S)-1-benzyl-2-(1-acetamido-3-ethyl) pentyl-3-hydrxoxymethyl- pyrrolidine-5-carboxylic acid t-butyl ester.

1H NMR (CDC13) : 8 9.70 (s, 1H), 7.22-7.36 (m 5H), 5.82 (br, 1H), 3.83 (dd, J= 3.3Hz, 13.5Hz, 2H), 3.74 (m, 1 H), 3.56 (d, J= 9Hz, 1H), 3.15 (m, 1H), 2.73 (m, 1H), 2.36-2.10 (m, 2H), 1.98 (s, 3H), 1.45 (s, 9H).

MS: (M+H)+= 361 204E (~)-(2R,3R,5R)-1-Benzyl-2-acetamidomethyl-3-methoxycarbonyl- pyrrolidine-5-carboxylic Acid t-Butyl Ester The title compound was prepared according to the method described in Example 2B and 2C, substituting (~)-(2R,3R,5R)-1-Benzyl-2-acetamidomethyl-3- formyl-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3R, 5R, 1'S)-1- benzyl-2- (1-acetamido-3-ethyl) pentyl-3-formyl-pyrrolidine-5-carboxylic acid t-butyl ester.

1H NMR (CDC13) : 8 7.45-7.20 (m, 5H), 5.96 (br, 1H), 3.90-3.73 (m, 4H), 3.71 (s, 3H), 3.52 (dd, J=9Hz, 2Hz, 1H), 3.13 (m, 1H), 2.84 (m, 1H), 2.36 (m, 1H), 2.18 (m, 1H), 1.97 (s, 3H), 1.45 (s, 9H).

MS: (M+H)+= 391 204F (~)-(2R,3R,5R)-2-Acetamidomethyl-3-methoxycarbonyl-pyrrolidi ne-5- carboxylic Acid t-Butyl Ester The title compound was prepared according to the method described in Example 2D, substituting ()- (2R, 3R, 5R)-1-benzyl-2-acetamidomethyl-3- methoxycarbonyl-pyrrolidine-5-carboxylic acid t-butyl ester in place of (~)- (2R, 3R, 5R, 1'S0-1-benzyl-2-(1-acetamido-3-ethyl)pentyl-3-methoxycarbony l- pyrrolidine-5-carboxylic acid t-butyl ester.

1H NMR (CDC13) : â 6.19 (br, 1 H), 3.72 (m, 2H), 3.70 (s, 3H), 3.43 (m, 1 H), 3.28 (m, 1H), 2.74 (m, 1 H), 2.44 (m 1H), 2.21 (m, 1H), 2.00 (s, 3H), 1.48 (s, 9H).

MS: (M+H)+= 301 204G (~)-(2R,3R,5R)-2-Acetamidomethyl-3-methoxycarbonyl-pyrrolidi ne-5- carboxylic Acid Hydrochloride.

The title compound was prepared according to the method described in Example 2E substituting ()- (2R, 3R, 5R)-2-acetamidomethyl-3-methoxycarbonyl- pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3R, 5R, 1'S)-2- (1- acetamido-3-ethyl) pentyl-3-methoxycarbonyl-pyrrolidine-5-carboxylic acid t-butyl ester.

'H NMR (D20): 8 4.42 (t, J=8.25Hz, 1H), 4.22 (m, 1 H), 3.83 (m, 1H), 3.75 (s, 3H), 3.70-3.60 (m, 2H), 3.26 (m, 1 H), 2.78 (m, 1 H), 2.43 (m, 1 H), 2.03 (s, 3H).

MS: (M+H)+= 245 Examples 205-213 HOm R I % AcHN_ OtBu b AcHNw OH Boc O H o Example 204C The following title compounds were prepared according to the methods described in Examples 1-39 from the common intermediate prepared as described in Example 204C.

Example 205 (~)-(2R,3R,5R)-2-Acetamidomethyl-3-ethoxycarbonyl-pyrrolidin e-5-carboxylic Acid Trifluoroacetic Acid Salt.

'H NMR (D20) 8 4.30 (t, J=8.2Hz, 1H), 4.21 (m, 3H), 3.62 (dd, J=2. 4, 3.4Hz, 2H), 3.23 (m, 1 H), 2.74 (m, 1 H), 2.38 (m, 1 H), 2.02 (s, 3H), 1.26 (m, 3H) MS : (M+H)'= 259; (M-H)'= 257.

Example 206 (~)-(2R,3R,5R)-2-Acetamidomethyl-3-(imidazol-2-yl)-pyrrolidi ne-5-carboxylic Acid Hydrochloride 'H NMR (D20): # 7.46 (s, 2H), 4.53 (dd, J=9.5Hz, J=8.5 Hz, 1 H), 4.28 (m, 1H), 3.96 (m, 1H), 3.65 (m, 2H), 3.03 (dt, J=13.5Hz, J=7.6Hz, 1H), 2.46 (m, 1H), 1.94 (s, 3H).

MS: (M+H) +=253, (M-H)-=251 Example 207 ()- (2R, 3S, 5R)-2-Acetamidomethyl-3-vinyl-pvrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt.

'H NMR (D20) 5 5.74 (m, 1H), 5.24 (m, 2H), 4.20 (dd, J=1.7,8.1Hz, 1H), 3.65 (m, 2H), 3.50 (m, 1 H), 2.84 (m, 1 H), 2.61 (m, 1H), 2.03 (s, 3H), 1.95 (m, 1H) MS: (M+H) + = 213.

Example 208 ()- (2R, 3R, 5R)-2-Acetamidomethyl-3- (2. 2-dimethyl-vinyl)-pvrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt.

1H NMR (D2O) # 5. 01 (br d, 1H), 4.18 (dd, J=2.1,8. 1Hz, 1H), 3.53 (m, 3H), 3.04 (m, 1 H), 2.55 (m, 1H), 2.0 (s, 3H), 1.75 (m, 1H), 1.72 (s, 3H), 1.67 (s, 3H) MS: (M+H)+= 241, (M+Na)+ = 263; (M-H)-= 239.

Example 209 (~)-(2R, 3R. 5R)-2-Acetamidomethyl-3- (N. N,N-dimethylcarbamoyl)-pyrrolidine-5- carboxylic Acid Trifluoroacetic Acid Salt.

'H NMR (D20) 8 4.60 (t, J=8.4Hz, 1 H), 4.23 (m, 1 H), 3.56 (d, J=5.8Hz, 2H) 3.50 (m, 1H), 3.10 (s, 3H), 2.94 (s, 3H), 2.88 (m, 1H), 2.19 (m, 1H), 2.00 (s, 3H) MS: (M+H) += 258, (M-H)-= 256.

Example210 (~)-(2R,3R,5R)-2-Acetamidomethyl-3-(N-methylcarbamoyl)-pyrro lidine-5- carboxylic Acid Trifluoroacetic Acid Salt.

'H NMR (D20) 4.49 (t, J=8.5Hz, 1H), 4.10 (m, 1H), 3.57 (d, J=5.8Hz, 2H), 3.03 (m, 1 H), 2.76 (m, 1 H), 2.74 (s, 3H), 2.29 (m, 1 H), 2.00 (s, 3H) MS: (M+H)+= 244.

Example 211 (~)- (2R. 3R, 5R)-2-Acetamidomethyl-3-propionyl-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt.

'H NMR (D20) 8 4.24 (m, 2H), 3.55 (d, J=4.7Hz, 1H), 3.40 (m, 1H), 2.85 (m, 1H), 2.64 (m, 3H), 2.16 (m, 1H), 2.01 (s, 3H), 1.02 (t, J=7. 1Hz, 3H) MS: (M+H)'= 243 ; (M-H)-= 241.

Example212 (~)-(2R,3R,5R)-2-Acetamidomethyl-3-methoxymethyl-pyrrolidine -5-carboxylic AcidHydrochloride 'H NMR (D2O) : 8 4.44 (t, J=6Hz, 2H), 3.77 (m, 1H), 3.65-3.48 (m, 3H), 3.35 (s, 3H), 2.64 (m, 1H), 2.56 (m, 1 H), 2.03 (s, 3H), 2.00 (m, 1H).

MS: (M+H)+= 231, (M-H)-= 229 Example 213 (~)-(2R,3R,5R)-2-Acetamidomethyl-3-methyl-pyrrolidine-5-carb oxylic Acid Trifluoroacetic Acid Salt.

'H NMR (D20) 8 4.30 (m, 1H), 3.64 (m, 1H), 3.48 (m, 1H), 3.20 (m, 1H), 2.64 (m, 1H), 2.03 (s, 3H), 1.76 (m, 1 H), 1.32 (br t, 1H), 1.12 (m, 4H) MS: (M+H)+= 201, (M+Na) + = 223.

Example 214 <BR> <BR> <BR> ()- (2R, 3S. 5R, 1'R)-2- (1-Acetamido-2-ethvlthio) ethvl-3-vinyl-pvrrolidine-5- carboxylic Acid Trifluoroacetic Acid Salt 214A (i)-(2R. 3S, 5R, 1'R)-1-t-Butoxycarbonyl-2-(1-t-butoxycarbonylamino-2- ethylthio) ethyl-3-vinyl-pyrrolidine-5-carboxylic Acid t-Butyl Ester.

To a solution of ethanethiol (0.047 mL, 0.63 mmol) in THF (2 mL) at 0°C was added 2.5 M n-BuLi/hexane (0.248 mL, 0.62 mmol). The reaction mixture was stirred for 45 minutes and a solution of ()- (2R, 3S, 5R, 1'S)-1-t- <BR> <BR> <BR> butoxycarbonyl-2- (N-t-butoxycarbonylaziridinyl)-3-vinyl-pyrrolidine-5-carboxy lic acid t-butyl ester (0.08 g, 0.182 mmole) in THF (0.5 mL) was added followed by DMF (1.5 mL) and stirred at room temperature for 2 hours. The reaction was quenched with 1 N NaHC03 (10 mL) and diluted with ethyl acetate (20 mL). The organic layer was washed with water, and brine, dried over MgS04, filtered and concentrated in vacuo. The residue was purified by chromatography on silica gel using 10% ethyl acetate/hexanes to provide the title compound (yield : 61 mg, 67%).

1 H NMR (d6-DMSO) 6 6.74 (br d, 1 H), 5.85 (m, 1 H), 4.9-5.0 (m, 2H), 4.20 (m, 1 H), 3.95 (m, 1 H), 3.75 (d,-1H), 2 : 8-3. 0 (dd, 1-H), 2.5 (m, 3H), 1.65 (m, 1H), 1.32-1.45 (m, 27H), 1.17 (dt, 3H).

MS : (M-H)'= 499 ; (M+H) + = 501, (M+Na) + = 523 214B (i)-(2R, 3S, 5R, 1'R)-1-t-Butoxvcarbonyl-2-(1-N-t-butoxvcarbonylacetamido- 2-ethylthio) ethyl-3-vinyl-pyrrolidine-5-carboxylic Acid t-but Ester.

()- (2R, 3S, 5R, 1'R)-1-Butoxycarbonyl-2-(1-N-t-butoxycarbonylamino-2- ethylthio) ethyl-3-vinyl-pyrrolidine-5-carboxylic acid t-butyl ester (58 mg, 0.116 mmole) was reacted with lithium hexamethyldisilazide (1 M) (1.16 mL, 1.16 mmole) in THF (3 mL) at-78 °C. After 0.5 hour at-78 °C and 1 hour at-40 °C, the above reaction mixture was reacted with acetyl chloride (0.166 mL, 2.33 mmole) at-30 °C for 0.3 hours. The reaction was quenched with 1 N NaHCO3 (10 mL) and extracted with ethyl acetate (20 mL). The organic layer was washed with water, and brine, dried over MgS04, filtered and concentrated in vacuo. The residue was purified by chromatography on silica gel using 10% ethyl acetate/hexanes to provide the title compound (yield: 28 mg, 44%).

1 H NMR (d6-DMSO) 8 5.88 (m, 1 H), 4.9-5.0 (m, 2H), 4.52 (m, 1 H), 4.33 (m, 1H), 4.1 (m, 1 H), 2.78 (dd, 1H), 2.3-2.5 (m, 6H), 1.7 (m, 1 H), 1.32-1.5 (m, 27H), 1.11 (t, 3H).

MS: (M+H) + = 543.

214C (~)-(2R,3R,5R,1'R)-2-(1-Acetamido-2-ethylthio)ethyl-3-vinyl- pyrrolidine-5- carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41 C, substituting ()- (2R, 3S, 5R, 1'R)-1-t-butoxycarbonyl-2- (1-N-t- <BR> <BR> <BR> <BR> butoxycarbonylacetamido-2-ethylthio) ethyl-3-vinyl-pyrrolidine-5-carboxylic acid t- butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1-acetamido- 2-hydroxy)butyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 7 mg, 95%).

1H NMR (d6-DMSO) 8 8.15 (d, 1H), 5.72 (m, 1H), 5.05-5.2 (m, 2H), 4.2- 4.4 (m, 2H), 4.33 (m, 1H), 2.93 (m, 1H), 2.7-2.8 (2d, 1H), 2.3-2.6 (m, 3H), 1.85- 1.95 (m, 1H), 1.93 (s, 3H), 1.17 (t, J=7.46 Hz, 3H) MS: (M+H) + = 287.

Example 215 ()- (2R, 3S, 5R. 1'R. 3'S)-2-1-Acetamido-2-ethvlsulfinyl) ethvl-3-vinyl-pyrrolidine-5- carboxylic Acid Trifluoroacetic Acid Salt 215A (i)-(2R. 3S. 5R. 1'R, 3'S) and ()- (2R, 3S. 5R. 1'R. 3'R)-1-t-Butoycarbonyl-2- (1-N-t-butoxycarbonylacetamido-2-ethylsulfinyl) ethyl-3-vinyl-prrrolidine-5- carboxylic Acid t-Butyl Ester.

()- (2R, 3S, 5R, 1'R)-1-t-Butoxycarbonyl-2- (1-N-t-butoxycarbonylacetamido- 2-ethylthio) ethyl-3-vinyl-pyrrolidine-5-carboxylic acid t-butyl ester (72 mg, 0.132 mmole) was reacted with 55% m-chloroperoxybenzoic acid (41 mg, 0.132 mmole) in CHC13 (1.5 mL) at -40 °C for 30 minutes. The reaction was concentrated in vacuo. The residue was purified by chromatography on silica gel using ethyl acetate to provide the title compounds ()- (2R, 3S, 5R, 1'R, 3'S) isomer (yield : 14 mg, 18.9%) and ()- (2R, 3S, 5R, 1'R, 3'R) (yield : 45 mg, 60.7%).

(2R, 3S, 5R, 1'R, 3'S) 1 H NMR (d6-DMSO) # 5.88 (m, 1H), 4.9-5.0 (m, 2H), 4.50 (m, 1H), 4.0-4.15 (m, 1H), 2.7-2.9 (m, 3H), 2.55 (m, 1H), 2.37 (s, 3H), 1.7 (m, 1H), 1.32-1.5 (m, 27H), 1.12 (t, 3H) MS: (M+H) + = 559, (M+Na) + = 581 (2R, 3S, 5R, 1'R, 3'R) 1 H NMR (d6-DMSO) 8 5.88 (m, 1H), 4.9-5.0 (m, 2H), 4.50 (m, 1H), 4.03-4.15 (m, 1H), 3.2 (m, 1H) 3.1 (dd, 1H), 2.5-2.7 (m, 2H), 2.38 (s, 3H), 1.75 (m, 1H), 1.32-1.5 (m, 27H), 1.12 (t, 3H) MS: (M+H) + = 559, (M+Na) + = 581 215B ()- (2R, 3S, 5R. 1'R. 3'S)-2-(1-acetamido-2-ethylsulfinyl)ethyl-3-vinyl- pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41C, substituting ()- (2R, 3S, 5R, 1'R, 3'S)-1-t-butoxycarbonyl-2- (1-N-t- <BR> <BR> <BR> <BR> butoxycarbonylacetamido-2-ethylsulfinyl) ethyl-3-vinyl-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t butoxycarbonyl-2- (1- <BR> <BR> <BR> <BR> acetamido-2-hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester. ester (yield : 9 mg, 86%).

1H NMR (d6-DMSO) 6 8.39 (d, 1H), 5.72 (m, 1H), 5.15-5.2 (dd, 2H), 4.5 (m, 1 H), 4.37 (m, 1 H), 3.65 (m, 1 H), 2.85-3.04 (m, 3H), 2.6-2.85 (m, 2H), 2.4 (m, 1H), 1.83-1. 95 (m, 1H), 1.86 (s, 3H), 1.20 (t, J=7.46 Hz 3H).

MS: (M-H)-=301 ; (M+H) + = 303, (M+Na)+= 325 Example 216 ()- (2R, 3S. 5R. 1'R. 3'R)-2-(1-Acetamido-2-ethylsulfinyl)ethyl-3-vinyl-pyrrolidin e-5- carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41 C, substituting ()- (2R, 3S, 5R, 1'R, 3'R)-1-t-butoxycarbonyl-2- (1-N-t- <BR> <BR> <BR> butoxycarbonylacetamido-2-ethylsulfinyl) ethyl-3-vinyl-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl 2-(1- acetamido-2-hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 12 mg, 94%).

1H NMR (d6-DMSO) 8 8.39 (d, 1 H), 5.72 (m, 1H), 5.15-5.2 (dd, 2H), 4.53 (m, 1H), 4.41 (t, 1H), 3.65 (m, 1H), 3.2 (dd, 1H), 2.9-3.0 (m, 2H), 2.65-2.9 (m, 2H), 2.4 (m, 1H), 1.83-1.95 (m, 1H), 1.83 (s, 3H), 1.20 (t, J=7.46 Hz, 3H) MS: (M-H)-=301 ; (M+H) + = 303, (M+Na) + = 325 Example 217 (~)-(2R,3R,5R,1'R)-1-(1-N-t-butoxycarbonylacetamido-2-ethyls ulfonyl)ethyl-3- vinyl-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 217A (i)-(2R. 3S, 5R. 1'R)-1-t-Butoxycarbonyl-2-(1-N-t-butoxycarbonvlacetamido- 2-ethvisulfonyl) ethyl-3-vinyl-pyrrolidine-5-carboxylic Acid t-Butyl Ester.

()- (2R, 3S, 5R, 1'R, 3'R)-1-t-Butoxycarbonyl-2- (1-N-t- <BR> <BR> <BR> butoxycarbonylacetamido-2-ethylsulfinyl) ethyl-3-vinyl-pyrrolidine-5-carboxylic acid t-butyl ester (25 mg, 0.0448 mmole) was reacted with 55% m- chloroperoxybenzoic acid (14 mg, 0.0448 mmole) in CHC13 (1.5 mL) at 0 °C for one hour. The reaction was concentrated in vacuo. The residue was purified by chromatography on silica gel using 25% ethyl acetate/hexane to provide the title compound (yield : 23.7 mg, 92%).

1H NMR (d6-DMSO) 5 5.88 (m, 1H), 4.85-5.0 (m, 2H), 4.38 (m, 1H), 4.15 (m, 1 H), 3.7 (m, 1 H) 3.45 (dd, 1 H), 2.9-3.2 (m, 3H), 2.5-2.7 (m, 1H), 2.3-2.4 (m, 3H), 1.6-2.04 (m, 1H), 1.35-1.55 (m, 27H), 1.15 (t, 3H) MS: (M+H) + = 575 217B()- (2R, 3S. 5R, 1'R)-2- (1-Acetamido-2-ethylsulfonyl) ethyl-3-vinyl-pyrrolidine- 5-carboxviic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41 C, substituting ()- (2R, 3S, 5R, 1'R)-1-t-butoxycarbonyl-2- (1-N-t- butoxycarbonylacetamido-2-ethylsulfonyl) ethyl-3-vinyl-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- <BR> <BR> <BR> acetamido-2-hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 12 mg, 94%).

1H NMR (d6-DMSO) 8 8.34 (d, 1 H), 5.72 (m, 1 H), 5.05-5.25 (dd, 2H), 4.68 (m, 1 H), 4.39 (dd, 1 H), 3.7 (2d, 1 H), 3.48 (dd, 1 H), 3.3-3.4 (dd, 2H), 3.08 (q, 2H), 2.95 (m, 1 H), 2.42 (m, 1 H), 1.9 (m, 1 H), 1.84 (s, 3H), 1.23 (t, J=7.46 Hz, 3H).

MS: (M-H)-= 317, (M+35) + = 353; (M+H) + = 319, (M+Na) + = 341 Examples 218, 220 \ 1. RSH . OtBu AcHN. ., OH 2. LiHMDS/AcCI'N Boc H goc s. TFA/CH2CI2 RS TFA The following title compounds were prepared in 3 steps according to the methods described in Example 214.

Example218 (~)-(2R,3R,5R,1'R)-2-(1-Acetamido-2-isopropylthio)ethyl-3-vi nyl-pyrrolidine-5- carboxvlic Acid Trifluoroacetic Acid Salt 218A (~)-(2R,3R,5R,1'R)-1-t-Butoxycarbonyl-2-(1-N-t-butoxycarbony lamino-2- isopropvithio) ethvl-3-vinyl-pvrrolidine-5-carboxvlic Acid t-Butvl Ester.

The title compound was prepared according to the method described in Example 214A, substituting isopropylthiol in place of ethanethiol (yield : 22 mg, 62%).

1 H NMR (d6-DMSO) 8 6.73 (d, 1H), 5.85 (m, 1H), 4.9-5.0 (m, 2H), 4.18 (m, 1 H), 3.95 (m, 1 H), 3.75 (br d, 1 H), 2.8-3.0 (m, 2H), 1.65 (m, 1 H), 1.32-1.45 (m, 27H), 1.18 (dd, 6H) MS: (M-H)-= 513; (M+H) + = 515, (M+Na) + = 537 218B (~)-(2R,3R,5R,1'R)-1-t-Butoxycarbonyl-2-(1-N-t-butoxycarbony l-N- acetamido)-2-isopropylthio) ethyl-3-vinyl-pyrrolidine-5-carboxvlic Acid t-But Ester.

The title compound was prepared according to the method described in Example 214B, substituting ()- (2R, 3S, 5R, 1'R)-1-t-Butoxycarbonyl-2- (1-N-t- butoxycarbonylamino-2-isoproylthio) ethyl-3-vinyl-pyrrolidine-5-carboxylic acid t- butyl ester in place of (i)-(2R, 3S, 5R, 1'R)-1-t-Butoxycarbonyl-2-(1-N-t- <BR> <BR> <BR> butoxycarbonylamino-2-ethylthio) ethyl-3-vinyl-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 12 mg, 50%).

1 H NMR (d6-DMSO) 8 5.86 (m, 1 H), 4.88-5.0 (m, 2H), 4.54 (m, 1 H), 4.33 (m, 1H), 4.13 (d, 1H), 3.05 (m, 1H), 2.73-2.84 (m, 2H), 2.38 (br s, 3H), 1.72 (m, 1H), 1.32-1.5 (m, 27H), 1.14 (dd, 6H).

MS: (M+H) + = 557, (M+Na) + = 579 218C ()- (2R, 3S, 5R, 1'R)-2- (l-Acetamido-2-isopropylthio) ethyl-3-vinyl pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 15B, substituting (~)-(2R,3R,5R,1'R)-1-t-butoxycarbonyl-2-(1-N-t- butoxycarbonylacetamido-2-isopropylthio) ethyl-3-vinyl-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- acetamido-2-hydroxy)butyl-3-(cis-propen-1-yl)-pyrrolidine-5- carboxylic acid t-butyl ester (yield : 8 mg, 97%).

1 H NMR (d6-DMSO) 5 8.14 (d, 1H), 5.72 (m, 1H), 5.05-5.2 (dd, 2H), 4.2- 4.4 (m, 2H), 3.68 (dd, 1 H), 2.93 (m, 2H), 2.74 (dd, 1H), 2.58 (dd, 1 H), 1.93 (m, 1 H), 1.87 (s, 3H), 1.2- (t, 6H) MS: (M-H)-= 299; (M+H) + = 301, (M+Na) + = 323 Example 219 <BR> <BR> <BR> (i)-(2R. 3S, 5R. 1'R)-2-(1-Acetamido-2-nhenylthio) ethvl-3-vinyl-pvrrolidine-5- carboxylic Acid Hvdrochloride 219A (~)-(2R,3S, 5R. 1'R)-1-t-Butoxycarbonyl-2- (1-amino-2-phenylthio) ethyl-3- vinyl-pyrrolidine-5-carboxylic Acid t-Butyl Ester.

()- (2R, 3S, 5R, 1'S)-1-t-Butoxycarbonyl-2-aziridinyl-3-vinyl-pyrrolidine-5- carboxylic acid t-butyl ester (20.3 mg, 0.06 mmole) was reacted with the phenylthiol (19.9 mg, 0.18 mmol) and triethylamine (0.047 mL, 0.34 mmol) in MeOH (0.06 mL) at ambient temperature for 3.5 hours. The reaction solution was concentrated in vacuo. The residue was purified by preparative thin layer chromatography on silica gel using ethyl acetate/methanol/ammonium hydroxide, 99/0.05/0.05, to provide the title compound (yield : 20.7 mg, 77%).

1H NMR (d6-DMSO) 8 7.31 (m, 4H), 7.17 (m, 1H), 5.87 (m, 1H), 5.03 (d, J=17Hz, 0.4H), 5.01 (d, J=17Hz, 0.6H), 4.91 (d, J=11H, 0.4H), 4.90 (d, J=11Hz, 0.6H), 4.15 (m, 1H), 3.82 (m, 0.6H), 3.76 (m, 0.4H), 3.39 (m, 1H), 2.92 (m, 2H), 2.55 (m, 1 H), 1.64 (m, 2H), 1.42 (s, 5.4H), 1.37 (s, 3.6H), 1.34 (s, 5.4H), 1.22 (s, 3.6H) MS: (M+H)+= 449, (M+Na) + = 471 219B (~)-(2R,3R,5R,1'R)-1-t-Butoxycarbonyl-2-(1-acetamido-2-pheny lthio)ethyl- 3-vinyl-pyrrolidine-5-carboxylic Acid t-Butyl Ester.

()- (2R, 3S, 5R, 1'R)-1-t-Butoxycarbonyl-2- (1-amino-2-phenylthio) ethyl-3- vinyl-pyrrolidine-5-carboxylic acid t-butyl ester (17.2 mg, 0.04 mmole) was reacted with the acetic anhydride (0.011 mL, 0.11 mmol) and triethylamine (0.032 mL, 0.23 mmol) in CH2CI2 (0.3 mL) at rt for 4.25 hours. The reaction solution was concentrated in vacuo. The residue was purified by preparative thin layer chromatography on silica gel using 5% methanol/dichloromethane to provide the title compound.

'H NMR (d6-DMSO) d 7.75 (d, J=9Hz, 0.6H), 7.73 (d, J=9Hz, 0.4H), 7.32 (m, 4H), 7.19 (m, 1H), 5.87 (m, 1H), 5.04 (d, J=17Hz, 0.4H), 5.00 (d, J=17Hz, 0.6H), 4.95 (d, J=10Hz, 0.6H), 4.93 (d, J=10Hz, 0.4H), 4.59 (m, 0.4H), 4.45 (m, 0.6H), 3.99 (dd, J=10Hz, 2Hz, 0.6H), 3.94 (dd, J=10Hz, 2.5Hz. 0.4H), 3.84 (m, 0.6H), 3.77 (m, 0.4H), 3.07 (dd, 13Hz, 5Hz, 0.6H), 2.95 (m, 1.8H), 2.83 (br t, J=8Hz, 0.6H), 2.48 (m, 1 H), 1.84 (s, 1.2H), 1.81 (s, 1.8H), 1.68 (m, 1 H), 1.41 (s, 5.4H), 1.36 (s, 3.6H), 1.34 (s, 5.4H), 1.26 (s, 3.6H) MS: (M-H)-= 489, (M+35)- ; (M+H) + = 490, (M+Na) + = 513 219C ()- (2R, 3S. 5R, 1'R)-2- 1-Acetamido-2-phenylthio) ethyl-3-vinyl-pyrrolidine-5- carboxylic Acid Hydrochloride The title compound was prepared according to the method described in Example 1K, substituting ()- (2R, 3S, 5R, 1'R)-1-t-butoxycarbonyl-2- (1-acetamido- 2-phenylthio) ethyl-3-vinyl-pyrrolidine-5-carboxylic acid t-butyl ester in place of (~)- (2R, 3R, 5R, 1'S)-2- (1-acetamido-3-ethyl) pentyl-3- (methoxymethyl)-pyrrolidine-5- carboxylic acid t-butyl ester (yield : 14.6 mg, 100%.) 1H NMR (d4-methanol) 8 7.43 (m, 2H), 7.31 (m, 3H), 5.75 (ddd, J=17Hz, 10Hz, 8Hz, 1H), 5.32 (brd, J=17Hz, 1H), 5.19 (dd, J=10Hz, 1.4Hz, 1H), 4.58 (m, 2H), 3.89 (dd, J=10Hz, 3Hz, 1H), 3.19 (dd, J=14Hz, 6Hz, 1H), 3.09 (dd, J=14Hz, 9Hz, 1H), 3.04 (m, 1H), 2.57 (dt, J=13Hz, 7Hz, 1H), 2.04 (s, 3H), 2.03 (m, 1H) MS : (M-H)-= 333; (M+H) + = 335, (M+Na) + = 357 Example 220 (~)-(2R,3R,5R,1'R)-2-(1-Acetamido-2-benzylthio)ethyl-3-vinyl -pyrrolidine-5- carboxylic Acid Trifluoroacetic Acid Salt 220A(i)-(2R, 3S. 5R, 1'R)-1-t-Butoxycarbonyl-2-(1-N-t-butoxvcarbonylamino-2- benzylthio) ethyl-3-vinyl-pvrrolidine-5-carboxylic Acid t-Butyl Ester The title compound was prepared according to the method described in Example 214A, substituting benzylmercaptan in place of ethanethiol (yield : 28 mg, 72%).

1 H NMR (d6-DMSO) 8 7.2-7.35 (m, 5H), 6.80 (br d, 1 H), 5.84 (m, 1 H), 4.86-4.96 (m, 2H), 4.25 (m, 1 H), 3.95 (m, 1 H), 3.7-3.8 (m, 3H), 2.76-2.94 (m, 1 H), 2.35-2.45 (m, 2H), 1.65 (m, 1 H), 1.32-1.45 (m, 27H) MS: (M-H)-= 561; (M+H) + = 563, (M+Na) + = 585 220B (~)-(2R,3R,5R,1'R)-1-t-Butoxycarbonyl-2-(1-(N-t-butoxycarbon yl- <BR> <BR> <BR> acetamido)-2-benzylthio) ethyl-3-vinyl-pvrrolidine-5-carboxylic Acid t-Butyl Ester.

The title compound was prepared according to the method described in Example 214B, substituting ()- (2R, 3S, 5R, 1'R)-1-t-Butoxycarbonyl-2- (1-N-t- butoxycarbonylamino-2-benzylthio) ethyl-3-vinyl-pyrrolidine-5-carboxylic acid t- butyl ester in place of ()- (2R, 3S, 5R, 1'R)-1-t-Butoxycarbonyl-2- (1-N-t- butoxycarbonylamino-2-ethylthio) ethyl-3-vinyl-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 3.3 mg, 61%).

1 H NMR (d6-DMSO) 8 7.2-7.35 (m, 5H), 5.84 (m, 1 H), 4.86-4.96 (m, 2H), 4.55 (m, 1 H), 4.32 (d, 1 H), 4.05 (d, 1 H), 3.56-3.65 (m, 2H), 2.9 (m, 1 H), 2.3-2.65 (m, 3H), 2.42 (s, 3H), 1.76 (d, 1 H), 1.25-1.55 (m, 27H) MS: (M+H) + = 605, (M+Na) + = 627 220C (~)-(2R,3R,5R,1'R)-2-(1-Acetamido-2-benzylthio)ethyl-3-vinyl -pyrrolidine-5- carboxytic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41C, substituting ()- (2R, 3S, 5R, 1'R)-1-t-butoxycarbonyl-2-(1-N-t- <BR> <BR> butoxycarbonylacetamido-2-benzylthio) ethyl-3-vinyl-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- acetamido-2-hydroxy) butyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 2.2 mg, 95%).

1 H NMR (d6-DMSO) # 8. 18 (d, 1 H), 7.2-7.32 (m, 5H), 5.68 (m, 1 H), 5.02- 5.2 (m, 2H), 4.3-4.45 (m, 2H), 3.76 (s, 2H), 3.68 (dd, 1 H), 2.92 (m, 1 H), 2.62 (dd, 1 H), 2.32-2.55 (m, 2H), 1.85-1.95 (m, 1 H), 1.89 (s, 3H).

MS: (M-H)-= 347; (M+H) + = 349, (M+Na) + = 371 Example 221 (i)-(2R, 3S, 5R, 1'R)-2-(1-Acetamido-2-(4-pyridinethio ! ethyl-3-vinyl-pyrrolidine-5- carboxylic Acid Dihydrochloride.

The title compound was prepared according to the method of Example 219A-C substituting 4-thiopyridine for thiophenol as the reagent in Example 219A.

'H NMR (d4-methanol) d 8.57 (d, J=7Hz, 2H), 7.97 (d, J=7Hz, 2H), 5.85 (ddd, J=17Hz, 10Hz, 9Hz, 1 H), 5.40 (br d, J=17Hz, 1H), 5.25 (dd, J=17Hz, 10Hz, 1H), 4.67 (dt, J=10Hz, 4Hz, 1H), 4.47 (dd, J= OHz, 8Hz, 1 H), 4.01 (dd, J=10Hz, 4Hz, 1H), 3.68 (dd, J=14Hz, 5Hz, 1H), 3.45 (dd, J=14Hz, 10Hz, 1H), 3.16 (m, 1 H), 2.65 (dt, J=14Hz, 7Hz, 1 H), 2.07 (m, 1 H), 2.04 (s, 3H) MS: (M-H)-= 334; (M+H) + = 336, (M+Na) + = 358 Example 222 ()- (2R, 3S. 5R, 1'R. 2'S)-2-1-Acetamido-2-hydrox nl-3- (cis-propen-1-yl)- pyrrolidine-5-carboxvlic Acid Ethyl Ester.

Thionyl chloride (1.49 mL, 20.5 mmol) was reacted with ethanol (25 mL) at 0°C for 10 minutes. ()- (2R, 3S, 5R, 1'R, 2'S)-2-(1-Acetamido-2-hydroxy)butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid trifluoroacetic acid salt (815 mg, 2.05 mmol) in ethanol (50 mL) was added to the above solution and reacted at room temperature for 17 hours. The reaction was concentrated in vacuo and the residue was purified by chromatography on silica gel with 90/10/0.5 dichloromethane/methanol/ammonium hydroxide to provide the title compound as a white solid (yield : 462 mg, 72%).

'H NMR (DMSO-d6) 8 7.49 (d, J = 9.8 Hz, 1 H), 5.31 (m, 2H) 4.11 (m, 2H), 3.72 (t, J = 7.7 Hz, 1 H), 3.69 (m, 1 H), 3.42 (m, 1 H), 3.07 (m, 1 H), 2.85 (m, 1 H), 2.22 (m, 1 H), 1.76 (s, 3H), 1.54 (d, J = 5.6 Hz, 3H), 1.45 (m, 1 H), 1.39 (m, 1 H), 1.21 (m, 1 H), 1.19 (t, J = 70 Hz, 3H), 0.83 (t, J=7. 3Hz, 3H).

MS : (M+H) += 313, (M+Na) += 335, (M-H)-= 311.

Example 223 (~)-(2R,3R,5R,1'R,2'S)-2-(1-Acetamido-2-hydroxy)butyl-3-(pyr azol-3-yl)- pyrrolidine-5-carboxylic Acid Ethvl Ester The title compound is prepared according to the method described in Example 222, substituting (~)- (2R, 3R, 5R, 1'R, 2'S)-2- (1-acetamido-2- hydroxy) butyl-3- (pyrazol-3-yl)-pyrrolidine-5-carboxylic acid trifluoroacetic acid salt in place of ()- (2R, 3S, 5R, 1'R, 2'S)-2-(1-acetamido-2-hydroxy)butyl-3-(cis-propen- 1-yl)-pyrrolidine-5-carboxylic trifluoroacetic acid salt (yield : 32 mg, 52%).

1H NMR(d6-DMSO) # 7. 6 (br s, 1H), 6.1 (br s, 1H), 4.08 (q, J=7.12Hz, 2H), 3.78 (m, 1H), 3.65 (m, 1H), 3.55 (m, 1H), 3.45 (m, 1H), 3.25 (m, 1H), 3.45 (m, 1H), 1.72 (s, 3H), 1.45 (m, 1H), 1.2 (m, 1H), 1.16 (t, J=7.12 Hz, 3H), 0.82 (t, J=7.46 Hz, 3H).

MS: (M-H)-= 337, (M+35) + = 373; (M+H) + = 339, (M+Na)+ = 361 Example 224 (i)-(2R. 3S, 5R, 1'S, 3'S)-2-(1-Acetamido-2-(N-isoPropyl-N-methylamino-N oxide))ethyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic Acid Ethyl Ester The title compound is prepared according to the method described in Example 222, substituting ()- (2R, 3S, 5R, 1'S, 3'S)-2-(1-acetamido-2-(N-isopropyl)- N-methylamino-N-oxide))ethyl-3-(cis-propen-1-yl)-pyrrolidine -5-carboxylic acid trifluoroacetic acid salt in place of ()- (2R, 3S, 5R, 1'R, 2'S)-2- (1-acetamido-2- hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid trifluoroacetic acid salt (yield : 25 mg, 34%).

1 H NMR (MeOD-d3) 5 5.51-5.43 (m, 1 H), 5.34-5.27 (m, 1 H), 4.36-4.30 (m, 1 H), 4.18 (q, J=7. 1Hz, 2H), 3.88 (dd, J=6.8,8.8Hz, 1H), 3.82-3.67 (m, 2H), 3.49- 3.42 (m, 1H), 3.34 (s, 3H), 3.14-2.96 (m, 1H), 2.42-2.33 (m, 1H), 1.92 (s, 3H), 1.64- 1.52 (m, 1H), 1.63 (dd, J=1.7,6.8Hz, 3H), 1.41-1.24 (m, 1H), 1.39 (d, J=6.4Hz, 3H), 1.31 (d, J=6.4Hz, 3H), 1.26 (t, J=7. 1Hz, 3H).

MS: (M+H)+=356, (M+Na)+=378, (M-H)-=354, (M+35) +=390.

Example 225 (~)-(2R,3R,5R,1'S)-2-(1-Acetamido-3-methyl)butyl-3-(cis-prop en-1-yl)-pyrrolidine- 5-carboxylic Acid Ethyl Ester The title compound is prepared according to the method described in Example 222, substituting ()- (2R, 3S, 5R, 1'S)-2-(1-acetamido-3-methyl(butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid trifluoroacetic acid salt in place of ()- (2R, 3S, 5R, 1'R, 2'S)-2-(1-acetamido-2-hydroxy)butyl-3-(cis-propen-1-yl)- pyrrolidine-5-carboxylic acid trifluoroacetic acid salt (yield : 838 mg, 94%).

1 H NMR (CDC13) : 8 5.50 (m, 1 H), 5.41 (m, 1 H), 5.28 (m, 1 H), 4.21 (q, J=7.5Hz, 2H), 4.06 (m, 1H), 3.87 (t, J=7.5Hz, 1H), 3.10 (m, 1H), 2.97 (m, 1H), 2.39 (m, 1H), 1.97 (s, 3H), 1.66 (dd, 3H), 1.60 (m, 1H), 1.40 (m, 2H), 0.94 (d, J=7.5Hz, 3H), 0.93 (d, J=7.5 Hz, 3H).

MS: (M+H) +=311 Example 226 (~)-(2R,3R,5R,1'S)-2-(1-Acetamido-3-methyl)butyl-3-(cis-2-ch loro-vin-1-yl)-= pyrrolidine-5-carboxylic Acid Ethyl Ester The title compound is prepared according to the method described in Example 222, substituting ()- (2R, 3S, 5R, 1'S)-2-(1-acetamido-3-methyl)butyl-3- (cis-2-chloro-vin-1-yl)-pyrrolidine-5-carboxylic acid trifluoroacetic acid salt in place of ()- (2R, 3S, 5R, 1'R, 2'S)-2- (1-acetamido-2-hydroxy) butyl-3- (cis-propen-1-yl)- pyrrolidine-5-carboxylic acid trifluoroacetic acid salt (yield : 28 mg, 46%).

1H NMR (CDC13) : # 6.05 (d, J=7.5Hz, 1H), 5.90 (dd, J1=9 Hz, J2=6Hz, 1H), 5.31 (d, J=9Hz, 1H), 4.19 (q, J=7.5Hz, 2H), 4.06 (m, 1H), 3.82 (t, J=7.5Hz, 1H), 3.17 (m, 2H), 2.45 (m, 1H), 1.98 (s, 3H), 1.67 (m, 1H), 1.60 (m, 1H), 1.37 (m, 2H), 1.27 (t, J=7.5Hz, 3H), 0.91 (d, J=7.5Hz, 3H), 0.89 (d, J=7.5Hz, 3H).

MS: (M+H) += 331 Example 227 Intentionally blank.

Example 228 (~)-(2R,3R,5R,1'S)-2-(1-Acetamido-3-methyl)butyl-3-(2,2-difl uoro-vinyl)- pyrrolidine-5-carboxylic Acid Ethyl Ester The title compound is prepared according to the method described in Example 222, substituting ()- (2R, 3S, 5R, 1'S)-2-(1-acetamido-3-methyl)butyl-3- (2,2-difluoro-vinyl)-pyrrolidine-5-carboxylic acid trifluoroacetic acid salt in place of ()- (2R, 3S, 5R, 1'R, 2'S)-2-(1-acetamido-2-hydroxy) butyl-3-(cis-propen-1-yl)- pyrrolidine-5-carboxylic acid trifluoroacetic acid salt (yield : 28 mg, 57%). rH NMR (CDC13) : 6 4.22 (q, J=7.5 Hz, 2H), 4.14 (m, 1H), 4.03 (m, 1H), 3.29 (br, 1 H), 2.85 (m, 1 H), 2.52 (m, 1 H), 2.01 (s, 3H), 1.77 (m, 2H), 1.64 (m, 2H), 1.49 (m, 1 H), 1.38 (m, 1 H), 1.29 (t, J=7.5Hz, 3H), 0.93 (d, J=7.5Hz, 3H), 0.90 (d, J=7.5Hz, 3H).

MS: (M+H) += 333 Example 229 (~)-(2R,3R,5R,1'S)-2-(1-Acetamido-3-methyl)butyl-3-(pyrazol- 3-yl)-pyrrolidine-5- carboxylic Acid Ethyl Este The title compound is prepared according to the method described in Example 222, substituting (~)-(2R, 3R, 5R, 1'S)-2-(1-acetamido-3-methyl)butyl-3- (pyrazol-3-yl)-pyrrolidine-5-carboxylic acid trifluoroacetic acid salt in place (~)- (2R, 3S, 5R, 1'R, 2'S)-2-(1-acetamido-2-hydroxy)butyl-3-(cis-propen-1-yl)- pyrrolidine-5-carboxylic acid trifluoroacetic acid salt (yield : 48 mg, 75.5%).

H NMR (CDC13) : 8 7.49 (d, 1 H), 7.26 (s, 1 H), 6.18 (d, 1H), 4.18 (q, J=7.5Hz, 2H), 4.12 (m, 1H), 3.91 (t, J=7.5Hz, 1H), 3.51 (t, J=7.5Hz, 1H), 3.40 (q, J=9Hz, 1H), 2.64 (m, 1H), 2.00 (m, 1H), 1.82 (s, 3H), 1.75 (m, 1H), 1.36 (m, 1H), 1.26 (t, J=9Hz, 3H), 0.855 (d, 3H), 0.84 (d, 3H).

MS : (M+H)+= 337 Example 230 (~)-(2R,3R,5R,1'R)-2-(1-Acetamido-2-ethyl-2-hydroxy)butyl-3- (cis-propen-1-yl)- pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt.

230A ()- (2R. 3S. 5R, 1'R)-1-t-Butoxycarbonyl-2- (1-acetamido-2-ethyl-2- hydroxy)butyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic Acid t-Butyl Ester.

The title compound was prepared according to the method described in Example 41B, substituting ()- (2R, 3S, 5R, 1'R)-1-f-butoxycarbonyl-2-(1-acetamido- 2-oxo) butyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester for (~)- (2R, 3S, 5R, 1'R)-1-t-butoxycarbonyl 2-(1-acetamido-1-formyl) methyl-3-(cis-propen- 1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester to provide the title compound (yield : 0.021 g, 51%).

MS: (M+H) += 469, (M+Na) + = 491, (2M+Na) +=959, (M-H)-= 467. 230B (~)-(2R,3R,5R,1'R)-2-(1-Acetamido-2-ethyl-2-hydroxy)butyl-3- (cis-propen- 1-vI)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41C, substituting ()- (2R, 3S, 5R, 1'R)-1-t-butoxycarbonyl-2-(1- <BR> <BR> acetamido-2-ethyl-2-hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- acetamido-2-hydroxy)butyl-3-(cis-propen-1-yl)-pyrrolidine-5- carboxylic acid t-butyl ester (yield : 0.0039 g, 100%).

'H NMR (DMSO-d6) 87. 52 (d, J=10.3Hz, 1H), 5.45 (m, 1H), 5.28 (m, 1H), 4.32 (m, 2H), 3.68 (t, J=8.8Hz, 1H), 3.16 (quint., J=8.5Hz, 1 H), 2.41 (dt, J=13. 2, 8.3Hz, 1H), 1.81 (s, 3H), 1.59 (m, 1H), 1.53 (dd, J=6.8,1.5Hz, 3H), 1.52- 1.42 (m, 3H), 1.30 (m, 1H), 0.86 (t, J=7.3Hz, 3H), 0.83 (t, J=7.3Hz, 3H).

MS: (M+H) + = 313, (M+Na) + = 335, (M-H)-= 311, (2M-H)-= 623.

Example 231 (~)-(2R, 3S. 5R. 1'R. 2'S)-2-(1-Acetamido-2-hvdroxv-2-methv))Dentvt-3-fc/s-DroDen- 1-yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt.

231A ()- (2R, 3S. 5R, 1'R, 2'S)-1-t-Butoxycarbony1-acetamido-2-hy-2-<BR> <BR> <BR> methvl) pentyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic Acid t-Butyl Ester.

The title compound was prepared according to the method described in Example 41B, substituting ()- (2R, 3S, 5R, 1'R)-1-t-butoxycarbonyl-2-(1-acetamido- 2-oxo) pentyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (fui)- (2R, 3S, 5R, 1'R)-1-t-Butoxycarbonyl 2- (1-acetamido-1-formyl) methyl-3- (cis- propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester and methylmagnesium bromide for ethylmagnesium bromide to provide the title compound (yield : 0.0285 g, 45%).

MS: (M+H)+= 469, (M+Na)+= 491.

231B (~)-(2R,3R,5R,1'S)-2-(1-Acetamido-2-hydroxy-2-methyl)pentyl- 3-(cis- propen-1-yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41C, substituting (~)-(2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- acetamido-2-hydroxy-2-methyl) pentyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- acetamido-2-hydroxy) butyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 0.0040 g, 100%).

1H NMR (DMSO-d6) 89. 25 (bs, 1H), 8.75 (bs, 1H), 7.54 (d, J=10. 3Hz, 1H), 5.45 (m, 1 H), 5.29 (m, 1 H), 4.37 (bt, J=8.3Hz, 1 H), 4.22 (t, J=9.7Hz, 1 H), 3.62 (t, J=8.8Hz, 1H), 3.12 (quint., J=8.5Hz, 1 H), 2.41 (dt, J=12. 7,7.8Hz, 1H), 1.78 (s, 3H), 1.59 (m, 1H), 1.53 (dd, J=6.8,2. 0Hz, 3H), 1.4-1.25 (m, 4H), 1.17 (s, 3H), 0.81 (t, J=6.5 Hz, 3H).

MS: (M+H) + = 313, (M+Na) + = 335, (M-H)-= 311, (2M-H)-= 623 Example 232 (~)-(2R,3R,5R,1'R,2'S)-2-(1-Acetamido-2-ethyl-2-hydroxy)pent yl-3-(cis-propen-1- v,)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt.

232A (~)-(2R,3R,5R,1'R,2'S)-1-t-Butoxycarbonyl-2-(1-acetamido-2-e thyl-2- hydroxy pentyl-3- (cis-aropen-1-yl)-pyrrolidine-5-carboxylic Acid t-Butvl Ester.

The title compound was prepared according to the method described in Example 41 B, substituting ()- (2R, 3S, 5R, 1'R)-1-t-butoxywarbonyl-2-(1-acetamido- 2-oxo) pentyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester for ()- (2R, 3S, 5R, 1'R)-1-t-butoxycarbonyl 2- (1-acetamido-1-formyl) methyl-3- (cis-propen- 1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester to provide the title compound (yield : 0.0222 g, 33%).

MS: (M+H)+= 483, (M+Na)+= 505, (M-H)-=481.

232B (~)-(2R,3R,5R,1'R,2'S)-2-(1-Acetamido-2-ethyl-2-hydroxy)pent yl-3-(cis- propen-1-yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41C, substituting ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- acetamido-2-ethyl-2-hydroxy) pentyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- acetamido-2-hydroxy)butyl-3-(cis-propen-1-yl)-pyrrolidine-5- carboxylic acid t-butyl ester (yield : 0.0035 g, 100%).

1H NMR (DMSO-d6) 8 9. 1 (bs, 1H), 8.75 (bs, 1H), 7.53 (d, J=9.8Hz, 1H), 5.44 (m, 1H), 5.28 (m, 1H), 4.35-4.25 (m, 2H), 3.67 (m, 1 H), 3.16 (quint., J=8.5Hz, 1H), 2.41 (dt, J=12.8,7.9Hz, 1H), 1.81 (s, 3H), 1.60 (m, 1H), 1.53 (dd, J=6.7,1.8Hz, 3H), 1.46 (m, 2H), 1.4-1.20 (m, 4H), 0.86 (t, J=7.3Hz, 3H), 0.82 (t, J=6.7 Hz, 3H).

MS: (M+H)+= 327, (M-H)-= 325, (M+CF3COOH)-=439, (2M-H)-= 651 Example 233 ()- (2R. 3S. 5R. 1'R)-2- (1-Acetamido-2-propyl-2-hydroxy) pentvl-3-fcis-propen-1-yl- pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 232 substituting propyl magnesium bromide for ethyl magnesium bromide.

1H) NMR (DMSO-d6) : 8 0.81 (t, 3H), 0.91 (t, 3H), 1.24-1.49 (m, 8H), 1.54 (dd, 3H), 1.60 (m, 1H), 1.81 (s, 3H), 2.41 (m, 1H), 3.15 (m, 1H), 3.69 (t, 1H), 4.28 (t, 1H), 4.35 (t, 1H), 5.17 (br s, 1H), 5.28 (td, 1H), 5.45 (dq, 1H), 7.54 (d, 1H), 8.80 (br s, 1 H), 9.12 (br s, 1 H).

MS: (M+H) += 341.

Example 234 (~)-(2R,3R,5R,1'R)-2-(1-Acetamido-2-ethyl-2-methoxy)butyl-3- (cis-propen-1-yl)- nvrrolidine-5-carboxYlic Acid Trifluoroacetic Acid Salt 234A (~)-(2R,3R,5R,1'R)-1-t-Butoxycarbonyl-2-(1-Acetamido-2-ethyl -2- (methylthio)methyloxy)butyl-3-(cis-propen-1-yl)-pyrrolidine- 5-carboxylic Acid t- Butyl Ester ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-Butoxycarbonyl-2-(1-acetamido-2-ethyl-2- methoxy) butyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester was reacted with dimethylsufoxide and acetic anhydride according to the method of Marshall, J. A. in J. Org. Chem. 1979, vol. 44, p 2994 to provide the title compound.

234A ()- (2R, 3S, 5R, 1'R, 2'S)-l-t-Butoxvcarbonyl-2- (l-Acetamido-2-ethyl-2- methoxy) butyl-3- (cis-propen-1-yl)-nyrrolid ine-5-carboxylic Acid t-Butyl Ester ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-Butoxycarbonyl-2-(1-acetamido-2-ethyl-2- (methylthio)methyloxy)butyl-3-(cis-propen-1-yl)-pyrrolidine- 5-carboxylic acid t- butyl ester is reacted with Raney Nickel according to the procedure of Marshall, J.

A. in J. Org. Chem. 1979, vol. 44, p 2994 to provide the title compound.

(~)-(2R,3S, 5R. 1'R, 2'S)-2-(1-Acetamido-2-ethyl-2-methoxy)butyl-3-(cis-propen-1- yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound is prepared according to the method described in Example 41C, substituting ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- acetamido-2-ethyl-2-methoxy) butyl-3-(cis-propen-1-yl)-pyrrolid ine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- acetamido-2-hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester.

Example 235 ()- (2R, 3S. 5R, 1'R. 2'S)-2-(1-Acetamido-2-ethyl-2-methoxy)pentyl-3-(cis-propen-1 - yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound is prepared according to the method described in Example 234 substituting ()- (2R, 3S, 5R, 1'R, 2'S)-1-f-butoxycarbonyl-2-(1- acetamido-2-ethyl-2-hydroxy)pentyl-3-(cis-propen-1-yl)pyrrol idine-5-carboxylic acid t-butyl ester for (~)-(2R, 3S, 5R, 1'R)-1-t-butoxycarbonyl-2- (1-acetamido-2- ethyl-2-hydroxy) butyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester in 234A.

Example 236 <BR> <BR> <BR> ()- (2R. 3S, 5R. 1'R. 2'S)-2- 1-Acetamido-2-hydroxymethvl-2-hydroxpentyl-3-ycis- propen-1-yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 236A (i !-(2R. 3S. 5R. 1'R. 2'S)-1-t-Butoxycarbonyl-2-(1-Acetamido-2-((1-<BR> <BR> <BR> <BR> <BR> ethoxv)ethyloxvmethyi-hydroxy pentyl-3- (cis-propen-1-y-pyrrolidine-5- carboxylic Acid t-Butyl Ester ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-Butoxycarbonyl-2-(1-acetamido-2-oxo)pentyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (50 mg, 0.11 mmole) was reacted with (ethoxyethyloxymethyl) tributylstannane (260 mg, 0.66 mmole) according to the method of Still, W. C. (J. Am. Chem. Soc., 100,1481 (1978)) to provide the title compound (yield: 26.8 mg, 43.8%). rH NMR (CDCl3) : 8 0.89 (t, 3H), 1.19 (m, 3H), 1.29 (dd, 3H), 1.45 (s, 9H), 1.46 (s, 9H), 1.52-1.73 (m, 8H), 1.99 (s, 3H), 2.44 (m, 1H), 3.24-3.74 (m, 5H), 3.91-4.22 (m, 3H), 4.49 (m, 1 H), 4.62 (m, 1 H), 5.37 (m, 1 H), 5.64 (m, 1 H), 5.97- 6.41 (m, 1H).

MS: (M+H) += 557.

236B (+)-(2R. 3S. 5R. 1'R. 2'S)-2-(1-Acetamido-2-hvdroxymethyl-2-hydroxv) pentyl- 3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-Butoxycarbonyl-2-(1-Acetamido-2-(1-ethoxy-2- ethoxymethyl)-2-hydroxy) pentyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic Acid t-Butyl Ester (13.5 mg, 0.024 mmol) was dissolved in THF (1 mL) and treated with 0.5 N HCI (1 mL) at room temperature for 1 hr. The solvents were removed and the resulting white solid was reacted with trifluoroacetic acid (0.8 mL) in dichloromethane (0.2 mL) at room temperature for 6 hours. The reaction was concentrated in vacuo overnight to provide the title compound (yield : 10.7 mg) as a off white solid.

'H NMR (DMSO-d6) : 8 0.81 (t, 3H), 1.24-1.38 (m, 4H), 1.52 (dd, 3H), 1.62 (m, 1H), 1.78 (s, 3H), 2.41 (m, 1H), 3.11 (m, 1H), 3.51 (qAB, 2H), 3.77 (t, 1H), 4.23 (t, 1 H), 4.40 (m, 1 H), 5.27 (t, 1 H), 5.45 (m, 1 H), 7.55 (d, 1 H), 8.87 (br s, 1 H), 9.26 (br s, 1 H).

MS : (M+H) += 329 Example 237 (~)-(2R,3R,5R,1'R,2'S)-2-(1-Acetamido-2-allyloxy-2-vinyl)eth yl-3-(cis-propen-1- vl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 237A (i)-(2R, 3S. 5R. 1'R. 2'S)-1-t-Butoxycarbonyl-2-(1-acetamido-2-allyloxy-2- vinyl)ethyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic Acid t-Butyl Ester (2R, 3S, 5R, 1'R, 2'S)-1-t-Butoxycarbonyl-2-(1-acetamido-2-hydroxy-2- vinyl) ethyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester was reacted according to the method described in Example 84A substituting allyl iodide for methyl iodide (yield : 28 mg, 80%).

MS: (M+H) += 479, (M-H)-= 477 237B (t)- (2R, 3S, 5R. 1'R. 2'S)-2- 1-Acetamido-2-allvloxy-2-vinyl ethyl-3- (cis- propen-1-yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41 C, substituting ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- acetamido-2-allyloxy-2-vinyl)ethyl-3-(cis-propen-1-yl)-pyrro lidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- acetamido-2-hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester. (yield : 4 mg, 100%).

1H NMR (DMSO-d6) 5 7.98 (d, J=7.8 Hz, 1H), 5.90 (m, 1H), 5.55 (m, 1H), 5.48 (m, 1H), 5.32 (m, 2H), 5.26 (m, 2H), 5.16 (m, 1H), 4.28 (m, 2H), 3.96 (m, 1 H), 3.79 (m, 1 H), 3.73 (m, 1 H), 3.66 (m, 1 H), 3.26 (m, 1 H), 2.40 (m, 1 H), 1.81 (s, 3H), 1.70 (m, 1H), 1.64 (dd, J=6.9,1.5 Hz, 3H).

MS: (M+H)+= 323, (M-H)-= 321.

Example 238 <BR> <BR> <BR> <BR> <BR> (ffi)-(2R. 3S. 5R. 1'R. 2'S)-2-(1-Acetamido-1-(2. 5-dihydrofuran-2-vl)) methyl-3-(cis- pronen-1-yl)-pvrrolidine-5-carboxvlic Acid Trifluoroacetic Acid Salt 238A (~)-(2R,3R,5R,1'R,2'S)-2-(1-Acetamido-1-(2,5-dihydrofuran-2- yl)methyl-3- (cis-propen-1--pyrrolidine-5-carboxylic Acid t-Butyl Ester ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-Butoxycarbonyl-2-(1-acetamido-2-allyloxy-2- vinyl)ethyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (21 mg, 0.044 mmole) prepared according to the procedure of Example 237A was reacted with bis (tricyclohexylphosphine) benzylidine ruthenium (IV) dichloride [Grubb's catalyst] (7.5 mg, 0.009 mmole) in methylene chloride (5 mL) at 25°C for 2 hours under a nitrogen atmosphere. The reaction was concentrated in vacuo and the resulting residue purified by chromatography on silica gel using 75% ethyl acetate/hexanes to provide the title compound (yield : 18 mg, 90%).

MS: (M+H)+= 451, (M-H)-= 449.

238B (~)-(2R,3R,5R,1'R,2'S)-2-(1-Acetamido-1-(2,5-dihydrofuran-2- yl))methyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41 C, substituting ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-Butoxycarbonyl-2-(1- acetamido-1- (2, 5-dihydrofuran-2-yi)) methyl-3- (cis-propen-1-yl)-pyrrolidine-5- carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl- <BR> <BR> <BR> 2- (1-acetamido-2-hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t- butyl ester. (yield : 7 mg, 100%).

1h NMR (DMSO-d6) 8 8.09 (d, J=8.8 Hz, 1H), 6.10 (m, 1H), 5.87 (m, 1H), 5.50 (m, 1 H), 5.27 (m, 1 H), 4.68 (m, 2H), 4.58 (m, 1 H), 4.33 (m, 1 H), 4.06 (m, 1H), 3.68 (m, 1H), 3.18 (m, 1H), 2.40 (m, 1H), 1.85 (s, 3H), 1.68 (m, 1H), 1.60 (dd, J=6.8,1.5 Hz, 3H), MS: (M+H) += 295, (M-H)-= 293.

Example 239 <BR> <BR> <BR> (i)-(2R, 3S, 5R, 1'R, 2'S)-2-(1-Acetamido-2-allyloxv-2-allyl) ethvl-3-(cis-nropen-1-yl)- pvrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 239A (~)-(2R,3R,5R,1'R,2'S)-1-t-Butoxycarbonyl-2-(1-acetamido-2-a llyloxy-2- allyl)ethyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic Acid t-Butyl Ester (2R, 3S, 5R, 1'R, 2'S)-1-t-Butoxycarbonyl-2-(1-acetamido-2-hydroxy-2- allyl) ethyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester was reacted according to the method described in Example 84A substituting allyl iodide for methyl iodide iodide (yield : 19 mg, 36%).

MS: (M+H) += 493, (M-H)'= 491.

239B ()- (2R. 3S. 5R, 1'R. 2'S)-2- (1-Acetamido-2-allyloxy-2-allyl) ethvl-3- (cis- propen-1-yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41C, substituting ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- <BR> <BR> <BR> Acetamido-2-allyloxy-2-allyl) propyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-Butoxycarbonyl-2-(1- acetamido-2-hydroxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester. (yield : 5.7 mg, 100%).

1H NMR (DMSO-d6) 8 8.06 (dd, J= 8.8 Hz, 1H), 6.92 (m, 1H), 6.77 (m, 1H), 5.50 (m, 1H), 5.29 (m, 2H), 5.17 (m, 1H), 5.05 (m, 2H), 4.27 (m, 2H), 4.10 (dd, J= 12.2,5.4 Hz, 1H), 3.83 (m, 1H), 3.78 (m, 1H), 3.40 (m, 1H), 3.20 (m, 1H), 2.46 (m, 1H), 2.38 (m, 1H), 2.20 (m, 1H), 1.88 (s, 3H), 1.69 (m, 1H), 1.63 (dd, J= 6.8,1. 5 Hz, 3H).

MS: (M+H) += 337, (M+Na) += 359, (M-H)-= 335.

Example 240 (~)-(2R,3S, 5R. 1'R 2'S)-2- (1-Acetamido-1- (3. 6-dihydro-2-H-pvran-2-yl)) methyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 240A (+)-(2R. 3S, 5R. 1'R. 2'S)-2-(1-Acetamido-1-(3,6-dihydro-2-H-pyran-2- yl)methyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic Acid t-Butyl Ester ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-Butoxycarbonyl-2- (1-Acetamido-2-allyloxy-2-allyl) ethyl- 3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (11.5 mg, 0.023 mmole) prepared according to the procedure of Example 239A was reacted with bis (tricyclohexylphosphine) benzylidine ruthenium (IV) dichloride [Grubb's catalyst] (3.8 mg, 0.005 mmole) in methylene chloride (3 mL) at 25°C for 3 hours under a nitrogen atmosphere. The reaction was concentrated in vacuo and the resulting residue purified by chromatography on silica gel using 75% ethyl acetate/hexanes to provide the title compound (yield : 5.7 mg, 53%).

MS: (M+H) += 465, (M+Na) += 487, (M-H)-= 463 240B (~)-(2R,3S, 5R. 1'R. 2'S)-2- (1-Acetamido-1- (3. 6-dihydro-2-H-pyran-2- yl)) methycis-propen-1-yl)-pyrrolidine-5-carboxvlic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41 C, substituting ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- acetamido-1- (3, 6-dihydro-2-H-pyran-2-yl)) propyl-3- (cis-propen-1-yl)-pyrrolidine-5- carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl- 2-(1-acetamido-2-hydroxy)butyl-3-(cis-propen-1-yl)-pyrrolidi ne-5-carboxylic acid t- butyl ester. (yield : 5.9 mg, 100%).

1H NMR (DMSO-d6) 8 8.04 (d, J= 8.8Hz, 1H), 5.77 (m, 2H), 5.50 (m, 1H), 5.25 (m, 1H), 4.21 (m, 2H), 4.14 (m, 1H), 4.04 (m, 1H), 3.81 (m, 1H), 3.40 (m, 1H), 3.23 (m, 1H), 2.41 (m, 1H), 2.09 (m, 1H), 1.88 (s, 3H), 1.83 (m, 1H), 1.70 (m, 1 H), 1.63 (d, J= 6. 8Hz, 3H).

MS: (M+h)+ = 309, (M+Na) + = 331, (M-H)-= 307 The following compounds were synthesized according to the methods previously described in Examples 1-240 Example 241 (~)-(2R,3R,5R,1'R,2'R)-2-(1-Acetamido-1-(3,6-dihydro-2-H-pyr an-2-yl))propyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 1H NMR (DMSO-d6) 6 7.90 (d, 9.1 Hz, 1 H), 5.79 (m, 2H), 5.48 (m, 1H), 5.23 (m, 1 H), 4.43 (m, 1H), 4.24 (m, 2H), 4.17 (m, 2H), 3.73 (m, 1H), 3.64 (m, 1H), 3.19 (m, 1H), 2.42 (m, 1H), 2.02 (m, 1H), 1.85 (s, 3H), 1.78 (m, 1H), 1.75 (m, 1 H), 1.56 (dd, J= 7. 5,1.5 Hz, 3H).

MS: (M+H) + = 309, (M+Na) + = 331, (M-H)-= 307.

Example 242 (~)-(2R,3R,5R,1'S,2'RS)-2-(1-Acetamido-2-hydroxy)pentyl-3-(c is-propen-1-yl)- pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 'H NMR (DMSO) 8 7.7 (d, J= 9.8 Hz, 1H), 5.61 (m, 1H), 5.19 (dt, J= 1.8, 11.0 Hz, 1H), 4.33 (dd, J= 6.7,10. 3 Hz, 1H), 3.81 (m, 1H), 3.70 (dd, 1.8,10.3 Hz, 1H), 3.54 (q, J= 6.1 Hz, 1H), 3.10 (m, 1H), 2.35 (dt, J= 12.8,6.8 Hz, 1H), 1.90 (s, 3H), 1.7 (m, 1 H), 1.59 (dd, J= 0.7,7.3 Hz, 3H), 1.4 (m, 3H), 1.2 (m, 2H), 0.90 (t, J= 6.7 Hz, 3H).

MS: (M+H)+= 299 Example 243 ()- (2R, 3S. 5R. 1'S, 2'RS)-2- (1-Acetamido-2-hydroxy-3-ethoxvcarbonyl propyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 'H NMR (DMSO) 8 7.75 (m, 1H), 5.60 (m, 1H), 5.29 (m, 1H), 4.55-4.25 (m, 3H), 4.15-4.0 (m, 3H), 3.9-3.6 (m, 3H), 3.15 (m, 1H), 2.45-2.3 (m 2H), 1.9 (s, 3H), 1.8-1.5 (m, 5H), 1.2 (m, 3H).

MS: (M+H)+= 343 Example 244 ()-f2R. 3S. 5R. 1'R. 2'S)-2-(1-Acetamido-2=-methoxy-2-vinyl)ethyl-3-(cis-propen-1 - yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 1H NMR (DMSO-d6) d 7.91 (d, J=8.05Hz, 1H), 5.50 (m, 2H), 5.30 (m, 3H), 4.27 (m, 1 H), 4.23 (m, 1 H), 3.75 (m, 1 H), 3.48 (m, 1 H), 3.23 (m, 1 H), 3.15 (s, 3H), 2.40 (m, 1H), 1.80 (s, 3H), 1.68 (m, 1H), 1.64 (dd, J=1.83,7.32Hz, 3H) MS: (M+H)'= 297, (M-H)-= 295 Example 245 ()- (2R, 3S, 5R. 1'R, 2'S)-2-(1-Acetamido-2-ethoxy-2-vinyl)ethyl-3-(cis-propen-1-y l)- pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 'H NMR (DMSO-d6) d 7.90 (d, J=7.85Hz, 1H), 5.57 (m, 2H), 5.48 (m, 3H), 4.27 (m, 1 H), 4.22 (m, 1H), 3.77 (m, 1H), 3.60 (m, 1H), 3.46 (m, 1H), 3.23 (m, 2H), 2.39 (m, 1H), 1.80 (s, 3H), 1.70 (m, 1H), 1.64 (dd, J=1.47,6.73Hz, 3H), 1.12 (t, J=6.83 Hz, 3H) MS: (M+H)+= 311, (M-H)- = 309 Example 246 (~)-(2R,3S, 5R. 1'R. 2'S)-2-(1-Acetamido-2-hydroxy-2-(propeny-2-yl))ethyl-3-(cis- propen-1-yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 1H NMR (DMSO-d6) 8 7.69 (d, J=9.75Hz, 1H), 5.47 (m, 1H), 5.28 (m, 1H), 5.03 (m, 1H), 4.86 (m, 1H), 4.40 (m, 1H), 4.30 (m, 1H), 4.18 (m, 1H), 3.97 (m, 1H), 3.68 (m, 1H), 3.21 (m, 1H), 2.43 (m, 1H), 1.82 (m, 1H), 1.73 (s, 3H), 1.64 (s, 3H), 1.59 (m, 3H) MS: (M+H)+= 297, (M-H)-= 295 Example 247 (~)-(2R,3R,5R,1'R,2'R)-2-(1-Acetamido-2-hydroxy-2-(propeny-2 -yl)ethyl-3-(cis- propen-1-yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 'H NMR (DMSO-d6) 8 7.65 (d, J=9.80 HZ, 1H), 5.48 (m, 1H), 5.23 (m, 1H), 4.99 (s, 1H), 4.88 (s, 1H), 4.46 (m, 1H), 4.30 (m, 1H), 4.19 (m, 1H), 3.55 (m, 1H), 3.22 (m, 1H), 2.44 (m, 1H), 1.78 (s, 3H), 1.75 (m, 1H), 1.65 (s, 3H), 1.58 (dd, J=1.23, 6.70HZ, 3H) MS: (M+H) += 297, (M-H)-=295 Example 248 (~)-(2R,3R,5R,1'R,2'S)-2-(1-Acetamido-2-methoxy-2-(propeny-2 -yl))ethyl-3-(cis- propen-1-yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 'H NMR (DMSO-d6) d 7.77 (d, J=9.8 Hz, 1H), 5.49 (m, 1H), 5.25 (m, 1H), 5.07 (m, 1H), 4.94 (m, 1H), 4.32 (m, 1H), 4.25 (m, 1H), 3.75 (m, 1), 3.48 (m, 1H), 3.25 (m, 1 H), 3.08 (s, 3H), 2.40 (m, 1 H), 1.77 (s, 3H), 1.68 (m, 1 H), 1.64 (dd, J=1.22, 6.71Hz, 3H), 1.56 (s, 3H) MS: (M+H) +=311, (M-H)-=309 Example 249 (~)-(2R,3R,5R,1'R)-2-(1-Acetamido-2-ethyl)butyl-3-(cis-prope n-1-yl)-pyrrolidine-5- carboxviic Acid Trifluoroacetic Acid Salt 'H NMR (DMSO-d6) 8 7.62 (d, J=9. 21Hz, 1H), 5.58 (m, 1H), 5.28 (m, 1H), 4.37 (m, 1 H), 3.98 (m, 1 H), 3.57 (m, 1 H), 3.10 (m, 1 H), 2.45 (m, 1 H), 1.92 (s, 3H), 1.76 (m, 1 H), 1.62 (dd, J=1.83,6.72Hz, 3H), 1.24 (m, 5H), 0.84 (t, J=7.61 Hz, 3H), 0.77 (t, J=7.61 Hz, 3H) MS: (M+H) +=297, (M-H)'=295 Example 250 (i)-(2 R, 3S. 5R, 1'S)-2-(1-Acetamido-2-ethyl) butyl-3-(cis-propen-1-yl)-pyrrolid ine-5- carboxylic Acid Trifluoroacetic Acid Salt 'H NMR (DMSO-d6) 8 7.76 (d, J=9.2 Hz, 1H), 5.46 (m, 1H), 5.29 (m, 1H), 4.23 (m, 1 H), 3.63 (m, 1 H), 3.15 (m, 1 H), 3.01 (m, 1 H), 2.38 (m, 1 H), 1.87 (s, 3H), 1.71 (m, 1H), 1.60 (m, 3H), 1.36 (m, 1H), 1.20 (m, 4H), 0.83 (t, J=7.3Hz, 6H) MS: (M+H) +=297, (M-H)-=295 Example 251 (-)-(2R, 3S. 5R. 1'S)-2-(1-Acetamido-2-hydroxy)butyl-3-(cis-propen-1-yl)- <BR> <BR> <BR> pvrrolidine-5-carboxvlic Acid Ethyl Ester and (+)-(2S, 3R, 5S, 1'R)-2-(1-Acetamido-<BR> <BR> <BR> 2-hyd roxv) butyl-3-(cis-propen-1-vl)-pyrrolidine-5-carboxylic Acid Ethyl Ester ()- (2R, 3S, 5R, 1'S)-2-(1-Acetamido-2-hydroxy)butyl-3-(cis-propen-1-yl)- pyrrolidine-5-carboxylic acid ethyl ester (100 mg) was chromatographed in one injection on a chiral HPLC column of dimensions 5 x 30 cm. The column was packed with Chiralpak AD chiral stationary phase packing from Chiral Technologies. The mobile phase consisted of 1: 9 ethanol : hexanes at a flow rate of 117 mUmin. Two peaks were observed at (24-36) minutes (-)- (2R, 3S, 5R, 1'S) (yield : 45 mg) and at (66-96) min (+)- (2S, 3R, 5S, 1'R) (yield : 45 mg).

(-)- (2R, 3S, 5R, 1'S) [a] D=-26° (c=0.78, dichloromethane) Example 252 (-)-(2R,3R,5R,1'S)-2-(1-Acetamido-2-hydroxy)butyl-3-(cis-pro pen-1-yl)- pyrrolidine-5-carboxylate Ammonium Salt (-)- (2R, 3S, 5R, 1'S)-2- (1-Acetamido-2-hydroxy) butyl-3- (cis-propen-1-yl)- pyrrolidine-5-carboxylic acid ethyl ester (4.9 mg, 0.0157 mmole) prepared according to the procedure of Example 251 was reacted with lithium hydroxide (0.75 mg, 0.0314 mmole) in a mixture of methanol (0.75 mL) and water (0.25 mL) at 0°C for 7 hours. Then 0.1 N aqueous Hydrochloric acid (1 mL) was added, the reaction was concentrated in vacuo and the resulting residue purified by ion exchange chromatography on Aldrich Dowex 50WX8-400 strongly acidic resin. The residue was placed on the column and washed with water (5 mL) followed by elution using 0.5 N aqueous Ammonium hydroxide to provide the title compound as a colorless solid (yield : 3.9 mg, 83%). [a] o =-40°, c=0.08 (water).

1 H NMR (DMSO-d6) 8 7.71 (d, J= 9.2 Hz, 1 H), 5.38 (m, 1 H), 5.29 (m, 1 H), 3.92 (m, 1H), 3.65 (t, J= 8.5 Hz, 1 H), 3.43 (m, 1H), 3.33 (m, 1H), 2.98 (m, 1H), 2.23 (m, 1 H), 1.76 (s, 3H), 1.54 (dd, J= 6.7,1.8 Hz, 3H), 1.46 (m, 2H), 1.23 (m, 1 H), 0.84 (t, J= 7. 3 Hz, 3H).

MS: (M+H) = 285, (M+Na) += 307, (M-H)-= 283.

[a] D =-40°, (c=0.08, water).

Example 253 (~)-(2R,3R,5R,1'R,2'S)-2-(1-Acetamido-2, 3-dimethoxv) propyl-3- (cis-propen-1-yl)- pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 1H NMR (MeOD-d3) 8. 7.8 (d, J=9.3Hz, 1H), 5.49-5.43 (m, 1H), 5.25 (dd, J=1.95,9.3Hz, 1H), 4.38-4.31 (m, 2H), 3.57-3.50 (m, 1H), 3.46 (dd, J=4.9,10.3Hz, 1H), 3.42 (s, 3H), 3.35-3.32 (m, 2H), 3.27 (s, 3H), 3.16-3.09 (m, 1H), 2.46-2.40 (m, 1H), 1.80 (s, 3H), 1.72-1.65 (m, 1H), 1.55 (d, J=6.8Hz, 3H).

MS: (M+H) +=315, (M+Na) +=337, (M-H)-=313, (M+Cl)-=349, (2M-H)-=627.

Example 254 (~)-(2R,3R,5R,1'R,2'S)-2-(1-Acetamido-2,3-dimethoxy)propyl-3 -(cis-proen-1-yl)- pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 'H NMR (MeOD-d3) 8. 8.04 (d, J=8.5Hz, 1H), 5.52-5.48 (m, 1H), 5.27- 5.22 (m, 1H), 4.32-4.25 (m, 2H), 3.74-3.71 (m, 1H), 3.53 (dd, J=2.4,10. 1Hz, 1H), 3.33-3.25 (m, 2H), 3.31 (s, 3H), 3.25 (s, 3H), 3.21-3.17 (m, 1H), 2.42-2.36 (m, 1H), 1.86 (s, 3H), 1.71-1.63 (m, 1 H), 1.62 (d, J=7.3Hz, 3H).

MS: (M+H) +=315, (M+Na) +=337, (M-H)-=313, (M+Cl)-=349, (2M-H)-=627 Example 255 (~)-(2R,3S, 5R. 1'R, 2'R)-2-(1-Acetamido-2-2-hydroxyethyl-2-hydroxy)pentyl-3-(cis - propen-1-yl)-pvrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 'H NMR (DMSO-d6) : 8 7.60 (m, 1H), 5.46 (m, 1H), 5.30 (m, 1H), 4.54 (m, 1H), 4. 35 (m, 1H), 4.03 (m, 1H), 3.96 (m, 1H), 3.69 (m, 1H), 3.15 (m, 1H), 2.40 (m, 1 H), 1.98 (m, 2H), 1.80 (s, 3H), 1.70-1.50 (m, 5H), 1.38 (m, 3H), 0.83 (m, 3H).

MS: (M+H) +=343, (M-H)'=341 Example 256 (~)- (2R. 3S, 5R, 1'R)-2-(1-Acetamido-2-(3-pentyloxy))ethyl-3-(cis-propen-1-yl )- pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 1H NMR (MeOD-d3) # 5.69-5.59 (m, 1H), 5.33-5.25 (m, 1H), 4.39 (m, 1H), 4.34 (dd, J=7.8,10.2Hz, 1H), 3.73 (dd, J=4.8,10.2Hz, 1H), 3.58-3.47 (m, 2H), 3.38-3.24 (m, 1 H), 3.27-3.20 (m, 1 H), 2.61-2.52 (m, 1 H), 2.02 (s, 3H), 1.90-1.78 (m, 1H), 1.70 (dd, J=1.7,6.8Hz, 3H), 1.60-1.50 (m, 4H), 0.92 (t, J=7.5Hz, 6H) (M+H) + = 327, (M+Na) + = 349 Example 257 (~)-(2R,3S,5R,1'S)-2-(1-Acetamido-2-(3-pentyloxy))ethyl-3-(c is-propen-1-yl)- pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 'H NMR (MeOD-d3) 8 5.73-5.66 (m, 1H), 5.32-5.25 (m, 1H), 4.36 (dd, J=7.8,10.2Hz, 1 H), 4.09 (m, 1H), 3.68 (dd, J=6.1,10.2Hz, 1H), 3.61 (d, J=4.4Hz, 2H), 3.35-3.23 (m, 1H), 3.24-3.16 (m, 1H), 2.65-2.55 (m, 1H), 2.03 (s, 3H), 1.92- 1.80 (m, 1H), 1.70 (dd, J=2.0,7.1 Hz, 3H), 1.59-1.47 (m, 4H), 0.94-0.88 (m, 6H) (M+H)+ = 327, (M+Na) + = 349 Example 258 (~)-(2R, 3S. 5R. 1'R, 2'S)-2-(1-Acetamido-2-ethoxy-3-vinyl)propyl-3-(cis-propen-1- yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt aH NMR (DMSO-d6) 8 8.01 (d, J= 8.6Hz, 1H), 5.76 (m, 1H), 5.49 (m, 1H), 5.25 (m, 1 H), 5.05 (m, 2H), 4.28 (m, 1 H), 4.02 (m, 1 H), 3.77 (m, 1 H), 3.62 (m, 1H), 3.36 (m, 1H), 3.29 (m, 1H), 3.18 (m, 1H), 2.43 (m, 1H), 2.38 (m, 1H), 2.16 (m, 1H), 1.87 (s, 3H), 1.69 (m, 1H), 1.63 (dd, J=6.7,1.2 Hz, 3H), 1.12 (t, J=6.7 Hz, 3H).

MS: (M+H) = 325, (M-H)-= 323 Example 259 (~)-(2R,3S,5R,1'R)-2-(1-Acetamido-2-allyloxy)ethyl-3-(cis-pr open-1-yl)- pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 'H NMR (DMSO-d6) 8 9.16 (m, 2H), 8.13 (d, J=7.5Hz, 1H), 5.88 (m, 1H), 5.50 (m, 1H), 5.15-5.32 (m, 3H), 4.35 (m, 2H), 3.95 (m, 2H), 3.61 (m, 1H), 3.40 (m, 2H), 3.20 (m, 1H), 2.40 (m, 1H), 1.87 (s, 3H), 1.72 (m, 1H), 1.62 (d, J=6.2,3H) MS: (M+1) =297, (M+23) =319, (2M+23) =615 Example 260 (~)-(2R,3S,5R, 1'R. 2'RS)-2-(1-Acetamido-2-hydroxy-2-(2-ethoxycarbonyl))pentyl- 3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 'H NMR (DMSO-d6) : 8 7.57 (d, J= OHz, 1 H), 5.45 (m, 1H), 5.29 (m, 1H), 4.35 (m, 1H), 4.09 (m, 1H), 3.68 (m, 1H), 3.44 (m, 1H), 3.17 (m, 1H), 2.87 (m, 1 H), 2.64 (m, 1H), 2.39 (m, 1H), 1.80 (s, 3H), 1.65-1.56 (m, 2H), 1.53 (m, 3H), 1.50-1.30 (m, 3H), 1.21 (t, J=7.5Hz, 3H), 0.80 (t, J=7.5Hz, 3H).

MS: (M+H)+=385, (M-H)-=383 Example 261 (i)-(2R. 3S. 5R. 1'S, 3R)-2-(1-Acetamido-3,4-dihydroxy)butyl-3-(cis-propen-1-yl)- pvrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 'H NMR (CD30D) 8 5.58-5.70 (m, 1H), 5.24-5.38 (m, 1H), 4.34-4.50 (m, 2H), 3.58-3.72 (m, 2H), 3.42-3.48 (d, 2H), 2.50-2.63 (m, 1 H), 2.04 (s, 3H), 1.77- 1. 95 (m, 1H), 1.65-1.76 (m, 4H), 1.50-1.63 (m, 1H).

MS: (M+H) += 301 Example 262 (~)-(2R,3S,5R,1'S,3'S)-2-(1-Acetamido-3,4-dihydroxy)butyl-3- (cis-propen-1-yl)- pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 1H NMR (CD30D) 8 5.58-5.72 (m, 1H), 5.25-5.37 (m, 1H), 4.30-4.45 (m, 2H), 3.63-3.77 (m, 2H), 3.44-3.49 (d, 2H), 2.50-2.63 (m, 1H), 2.03 (s, 3H), 1.76- 1.95 (m, 2H), 1.65-1.75 (m, 4H).

MS: (M+H) += 301 Example 263 <BR> <BR> <BR> <BR> (i)-(2R, 3S. 5R. 1'R)-2-(1-Acetamido-2-methoxy) ethyl-3-(cis-pronen-1-yl)- pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt.

263A (~)-(2R,3S,5R,1'R)-1-t-Butoxycarbonyl-2-(1-Acetamido-2-metho xy)ethyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic Acid t-Buyl Ester.

The title compound was prepared according to the method described in Example 84A, substituting ()- (2R, 3S, 5R, 1'R)-1-t-butoxycarbonyl-2- (1-acetamido- 2-hydroxy) ethyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester for ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1-acetamido-2-hydroxy) butyl-3-(cis- propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 4.2 mg, 20%).

MS: (M+H) +=427, (M+Na) +=449, (M-H)-=425.

263B (~)-(2R,3S,5R,1'R)-2-(1-Acetamido-2-methoxy)ethyl-3-(cis-pro pen-1-yl)- pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt.

The title compound was prepared according to the method described in Example 41 C, substituting ()- (2R, 3S, 5R, 1'R)-1-t-butoxycarbonyl-2-(1- acetamido-2-methoxy) ethyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t- butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-)-1-t-butoxycarbonyl-2-(1-acetamido- 2-hyd roxy) butyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 0.0031 g, 100%).

1H NMR (DMSO-d6) 8 8.12 (d, J=7.9Hz, 1H), 5.50 (m, 1 H), 5.23 (m, 1H), 4.33 (m 1H), 3.56 (dd, J=9.7,8. 0Hz, 1H), 3.4-3.3 (m, 2H), 3.26 (s, 3H), 3.19 (m, 1H), 2.39 (dt, J=12. 8,7.3Hz, 1H), 1.86 (s, 3H), 1.71 (m, 1H), 1.61 (dd, J=6.7,1.8Hz, 3H).

MS: (M+H)+=271, (M+Na)+=293.

Example 264 (i)-(2R. 3S. 5R. 1'R. 2'S)-2-(1-Acetamido-2-hydroxy-3-dimethyl)butyl-3-(cis-propen - 1-yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt.

264A (~)-(2R,3S,5R,1'R,2'S)-1-t-Butoxycarbonyl-2-(1-acetamido-2-h ydroxy-3- dimethyl)butyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic Acid t-Butyl Ester.

The title compounds were prepared according to the method described in Example 41 B, substituting t-butyl lithium for ethyl magnesium bromide to provide ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2- (1-acetamido-2-hydroxy-3- dimethyl)butyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 2.5 mg, 11 %) ()- (2R, 3S, 5R, 1'R, 2'S) MS: (M+H)+= 469; (M-H)'= 467.

264B (~)-(2R,3S,5R,1'R,2'S)-2-(1-Acetamido-2-hydroxy-4-vinyl)buty l-3-(cis- propen-1-yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The title compound was prepared according to the method described in Example 41 C, substituting ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- acetamido-2-hydroxy-3-dimethyl)butyl-3-(cis-propen-1-yl)-pyr rolidine-5-carboxylic acid t-butyl ester in place of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- acetamido-2-hydroxy) butyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic acid t-butyl ester (yield : 2.3 mg, 100%).

1H NMR (D20) 8 5.40 (m, 1H), 5.10 (t, J=5.5Hz, 1H), 4.13 (t, J=9.2Hz, 1H), 3.46 (m, 1H), 3.22 (d, J=7.3Hz, 1H), 3.00 (m, 1H), 2.41 (m, 1H), 1.70 (s, 3H), 1.45 (m, 1 H), 1.39 (d, J=4.9Hz, 3H), 1.07 (t, J=5.5Hz, 1H), 0.70 (s, 9H) MS: (M+H) + = 313.

Example265 <BR> <BR> <BR> <BR> <BR> ()- (2R, 3S. 5R. 1'R, 2'S)-2-(1-acetamido-2-methoxy-2-methyl) pentyl-3- (cis-propen- 1-vl) pyrrolidine-5-carboxvlic Acid Trifluoroacetic acid Salt 265A (+)-(2R. 3S. 5R. 1'R. 2'S)-1-t-Butoxycarbonyl-2-1-acetamido-2-methoxy-2- methypentyl-3- (cis-propen-1-yl) pvrrolidine-5-carboxylic Acid t-Butyl ester.

Sodium bis (trimethylsilyl) amide (7.26 mL, 1. OM in THF, 7.26 mmol) was added slowly to a solution of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2-(1- acetamido-2-hydroxy-2-methyl) pentyl-3- (cis-propen-1-yl) pyrrolidine-5-carboxylic acid t-butyl ester (3.09 g, 6.6 mmol) in THF (65 mL) at-78 °C. The reaction mixture was stirred at-78 °C for 1 h and then at 0 °C for 20 min. Methyl iodide (8.2 mL, 1. 8. 8 g. 0.132 mol) was added and the mixture stirred at 0 °C for 20 min and rt for 2 h. The reaction mixture was then quenched with saturated aqueous ammonium chloride (20 mL) and water (20 mL) and extracted using ethyl acetate (3 x 100 mL). The combined organic layer was dried over magnesium sulfate, filtered, and concentrated in vacuo. The residue was purified by column chromatography on silica gel using 99: 1 dichloromethane/methanol followed by 98: 2 dichloromethane/methanol to give the title compound (2.1 g, 67% yield).

This compound exists as a mixture of rotamers at rt. The'H NMR spectrum was therefore acquired at 130 °C.

1H NMR (DMSO-d6) 8 6.9 (bs, 1H), 5.75 (m, 1H), 5.3 (m, 1 H), 4.55 (m, 1H), 3.9 (m, 2H), 3.5 (m, 1H), 3.15 (s, 3H), 2.6 (m, 1H), 1.85 (s, 3H), 1.6 (d, J=6 Hz, 3H), 1.5-1.25 (m, 4H), 1.40 (bs, 18H), 1.08 (s, 3H), 0.85 (t, J=7 Hz, 3H).

MS: (M+H)+=483, (M+Na) +=505, (M-H)-= 481.

265B (_)-(2R. 3S. 5R. 1'R. 2'S)-2-(1-acetamido-2-methoxy-2-methyl) Pentyl-3-(cis- propen-1-yl) pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt.

()- (2R, 3S, 5R, 1'R, 2'S)-1-t-Butoxycarbonyl-2- (1-acetamido-2-methoxy-2- methyl) pentyl-3- (cis-propen-1-yl) pyrrolidine-5-carboxylic acid t-butyl ester (4.1 mg, 0.0085 mmol) was reacted with trifluoroacetic acid (0.8 mL) in dichloromethane (0.2 mL) at room temperature for 4 h. The reaction mixture was concentrated in vacuo overnight to provide the title compound (yield : 4.1 mg, 100 %) as a colorless oil.

'H NMR (DMSO-d6) 8 8.96 (bs, 1H), 8.61 (bs, 1H), 7.61 (d, J=10.3 Hz, 1 H), 5.45 (m, 1 H), 5.28 (m, 1 H), 4.33 (t, J=9.8Hz, 1 H), 4.29 (m, 1 H), 3.59 (m, 1H), 3.2-3.1 (m, 1H), 3.14 (s, 3H), 2.43 (dt, J=12. 8,7.9Hz, 1H), 1.80 (s, 3H), 1.6- 1.4 (m, 2H), 1.53 (dd, J=6.7,1.2Hz, 3H), 1.35-1.25 (m, 3H), 1.19 (s, 3H), 0.80 (t, J=6.7Hz, 3H).

MS: (M+H) +=327, (M+Na) +=349, (2M+H) +=653, (M-H)-=325, (2M-H)-=651.

Example 266 (_)-(2R. 3S, 5R, 1'R. 2'S)-2-(1-Acetamido-2-methoxy-2-methyl) pentvl-3-(cis-oropen-<BR> <BR> <BR> <BR> <BR> <BR> 1-yl) pvrrolidine-5-carboxylic Acid Ethyl Ester A mixture of 9.88 g (0.083 mol, 6.05 mL) thionyl chloride and 175 mL of anhydrous ethanol was stirred at rt for 15 min. To this mixture was added slowly dropwise a solution of 1.83 g (4.15 mmol) ()- (2R, 3S, 5R, 1'R, 2'S)-2- (1- <BR> <BR> <BR> <BR> acetamido-2-methoxy-2-methyl) pentyl-3- (cis-propen-1-yl) pyrrolidine-5-carboxylic acid trifluoroacetic acid salt in 25 mL of anhydrous ethanol. The mixture was stirred at room temperature for 18 h and then the solvent was evaporated in vacuo overnight. The residue was purified by column chromatography on silica gel using 98: 2: 0.1 dichloromethane/methanol/ammonium hydroxide to give the title compound (1.44 g, 98% yield) as a thick colorless oil which solidifie upon prolonge standing.

'H NMR (CDC13) 5.48 (d, J=10.8 Hz, 1H), 5.45-5.28 (m, 2H), 4.16 (m 2H), 3.83 (t, J=7.4Hz, 1H), 3.29 (t, J=7.1 Hz, 1 H), 3.17 (s, 3H), 2.97 (quintet, J=8. 1Hz, 1H), 2.31 (dt, J=12.9,7.8 Hz, 1H), 1.95 (s, 3H), 1.61 (dd, J=6.5,1.4Hz, 3H), 1.55- 1.30 (m, 3H), 1.26 (t, J=7.1 Hz, 3H), 1.11 (s, 3H), 0.90 (t, J=7. 1Hz, 3H).

MS: (M+H) +=355, (M-H)-= 353.

Example 267 (-)-(2R. 3S, 5R. 1'R. 2'S)L1-Acetamido-2-methoxy-2-methvl) pentyl-3-i (cis-propen-<BR> <BR> <BR> <BR> <BR> 1-yl) pyrrolidine-5-carboxylic Acid Ethyl Ester Resolution of the racemic ethyl ester was carried out by chiral HPLC using a Chiralpak AD column (10 micron particle diameter, Chiral Technologies, Exton, PA) eluting with 98: 2 (v/v) hexane/ethanol. Thus, ()- (2R, 3S, 5R, 1'R, 2'S)- 2- (l-acetamido-2-methoxy-2-methyl) pentyl-3- (cis-propen-1-yl) pyrrolidine-5- carboxylic acid ethyl ester (14.8 g, 0.042 mol) provided 6.44 g (44% recovery) of (-)- (2R, 3S, 5R, 1'R, 2'S)-2-(1-acetamido-2-methoxy-2-methyl)pentyl-3-(cis-propen- 1-yl) pyrrolidine-5-carboxylic acid ethyl ester ([a] =-76. 9° in dichloromethane at room temperature) and 6.34 g (43% recovery) of (+)-(2S,3R,5S, 1'S, 2'R)-2- (1- acetamido-2-methoxy-2-methyl) pentyl-3-(cis-propen-1-yl)pyrrolidine-5-carboxylic acid ethyl ester ([α] = +75.8° in dichloromethane at room temperature). The'H NMR and MS data for each of these compounds matched exactly the data obtained for the racemic mixture.

Example 268 (-)-(2R. 3S, 5R, 1'R, 2'S)-2-(1-Acetamido-2-methoxy-2-methyl) pentyl-3-(cis-propen- 1-yl)pyrrolidine-5-carboxylic Acid Ethyl ESter p-Toluenesulfonic Acid Salt A solution of (-)-(2R,3S, 5R, 1'R, 2'S)-2-(1-acetamido-2-methoxy-2-methyl)pentyl- 3- (cis-propen-1-yl) pyrrolidine-5-carboxylic acid ethyl ester (0.269 g, 0.759 mmol) and p-toluenesulfonic acid hydrate (0.144 g, 0.759 mmol) in dichloromethane (15 mL) was stirred at room temperature for 30 minutes. The solvent was then evaporated in vacuo to provide the title compound (yield : 0.390 g, 98%) as a white powder.

'H NMR 8 10.1 (bs, 1H), 9.4, (bs, 1H), 7.78 (d, J = 8.1 Hz, 2H), 7.16 (d, J=7.8 Hz, 2H), 6.75 (bd, J=10 Hz, 1H), 5.6-5.4 (m, 2H), 4.49 (t, J=9. 5 Hz, 1H), 4.28 (m, 1H), 4.22 (q, J=7.2 Hz, 2H), 4.14 (m, 1H), 3.32 (m, 1H), 3.24 (s, 3H), 2.48 (m, 2H), 2.35 (s, 3H), 1.94 (s, 3H), 1.93 (dt, J=13. 7,10.5 Hz, 1H), 1.57 (d, J=5.4 Hz, 3H), 1.5-1.4 (m, 3H), 1.37 (s, 3H), 1.26 (t, J=7.1 Hz, 3H), 0.85 (t, J=6.8 Hz, 3H).

MS: (M+H) + = 355, (M-H)- = 353.

Anal Calcd for C26H42N207S : C, 59.29; H, 8.04; N, 5.32; S, 6.09. Found: C, 59.24; H, 7.87; N, 5.16; S, 6.18 Example 269 ()- (2R, 3S. 5R, 1'R. 2'S)-2-1-acetamido-2-methoxy-2-methyl)pentyl-3- (cis-propen-<BR> <BR> <BR> <BR> <BR> <BR> 1-vl) pyrrolidine-5-carboxylic Acid Isopropyl Ester : A mixture of 0.19 g (1.59 mmol, 0.12 mL) thionyl chloride and 10 mL of 2- propanol was stirred at rt for 15 min. To this mixture was added slowly dropwise a solution of 0.35 g (0.0795 mmol) ()- (2R, 3S, 5R, 1'R, 2'S)-2- (1-acetamido-2- methoxy-2-methyl) pentyl-3- (cis-propen-1-yl) pyrrolidine-5-carboxylic acid trifluoroacetic acid salt in 2 mL of 2-propanol. The mixture was stirred at room temperature for 48 hours and then the solvent was evaporated in vacuo. The residue was purified by column chromatography on silica gel using 98: 2: 0.1 dichloromethane/methanol/ammonium hydroxide to provide the title compound (yield : 9.1 mg, 31%) as a colorless oil.

1H NMR (CDC13) 8 5.5-5.25 (m, 3H), 5.02 (septet, J=6.3 Hz, 1H), 4.15 (dd, J=10. 2,7.1 Hz, 1H), 3.79 (t, J=7.5 Hz, 1H), 3.28 (t, J=7.4 Hz, 1H), 3.17 (s, 3H), 2.96 (quintet, J=8.3 Hz, 1H), 2.30 (td, J=12.5,8.2 Hz, 1H), 1.94 (s, 3H), 1.62 (dd, J=6.8,1.6 Hz, 3H), 1.65-1.30 (m, 5H), 1.24 (d, J=6.5 Hz, 3H), 1.21 (d, J=6.3 Hz, 3H), 1.11 (s, 3H), 0.90 (t, J=7.1 Hz, 3H).

MS: (M+H) + = 369, (M-H)-= 367.

Example 270 (~)-(2R,3S,5R,1'R,2'S)-2-(1-acetamido-2-methoxy-2-methyl)pen tyl-3-(cis-propen- 1-vl) pyrrolidine-5-carboxylic Acid Isopropyl Ester p-Toluenesulfonic Acid Salt A mixture of ()- (2R, 3S, 5R, 1'R, 2'S)-2- (1-acetamido-2-methoxy-2- methyl)pentyl-3-(cis-propen-1-yl)pyrrolidine-5-carboxylic acid isopropyl ester (8.4 mg, 0.0228 mmol) and p-toluenesulfonic acid monohydrate (0.0043 g, 0.0228 mmol) in 1 mL of dichloromethane was stirred at rt for 1 h. The solvent was evaporated in vacuo to provide the desired product (yield : 12.0 mg, 97%) as a white powder. rH NMR (CDC13) 8 9.9 (bs, 1H), 9.45 (bs, 1H), 7.79 (d, J=8.1 Hz, 2H), 7.15 (d, J=7.8 Hz, 2H), 6.57 (bd, J=8.9 Hz, 1H), 5.53 (m, 2H), 5.07 (quintet, J=6.3 Hz, 1 H), 4.50 (t, J=9.5 Hz, 1 H), 4.25 (bs, 1H), 4. 10 (bs, 1H), 3.34 (m, 1H), 3.24 (s, 3H), 2.50 (m, 1 H), 2.35 (s, 3H), 1.95 (s, 3H), 1.9 (m, 1 H), 1.6-1.2 (m, 6H), 1.35 (s, 3H), 1.25 (d, J = 6.4 Hz, 3H), 1.23 (d, 3H), 0.85 (t, J = 7.0 Hz, 3H).

MS: (M+H) + = 369, (M-H)-= 367.

Example 271 -f2R.3S.5R.1'R.2'S)-2-(1-acetamido-2-methoxv-2-methv))Dentv) -3-(c/s-DroDen- 1-vl) pyrrolidine-5-carboxylic acid t-butvl ester: A mixture of ()- (2R, 3S, 5R, 1'R, 2'S)-1-t-butoxycarbonyl-2- (1-acetamido-2- methoxy-2-methyl) pentyl-3- (cis-propen-1-yl) pyrrolidine-5-carboxylic acid t-butyl ester (0.4123 g, 0.855 mmol) and trifluoroacetic acid (2.93 g, 25.7 mmol) in 10 mL dichloromethane was stirred at rt for 1.5 h. The solvent was evaporated in vacuo and the residue purified by chromatography on silica gel, eluting with 98: 2: 0.1 dichloromethane/methanol/ammonium hydroxide to provide the desired product (yield : 0.168 g, 51%) as a colorless oil.

'H NMR (CDC13) 8 5.5-5.25 (m, 3H), 4.15 (dd, J=10.1,7.2 Hz, 1H), 3.73 (t, J=7.4 Hz, 1H), 3.26 (t, J=7.1 Hz, 1 H), 3.17 (s, 3H), 2.94 (quintet, J=8.3 Hz, 1H), 2.28 (td, J=12. 5, 7.8 Hz, 1 H), 1.93 (s, 3H), 1.61 (dd, J=6.4,1.4 Hz, 3H), 1.58-1.20 (m, 5H), 1.44 (s, 9H), 1.11 (s, 3H), 0.90 (t, J=7.1 Hz, 3H).

MS: (M+H)+ = 383, (M-H)- = 381.

Example 272 f)- (2R, 3S, 5R, 1'R. 2'S)-2-n-acetamido-2-methoxv-2-methv))Dentv)-3-fc/s- <BR> <BR> propen-1-yl) pyrrolidine-5-carboxylic Acid t-Butyl ester p-Toluenesulfonic Acid Salt : A mixture of ()- (2R, 3S, 5R, 1'R, 2'S)-2- (1-acetamido-2-methoxy-2- methyl) pentyl-3- (cis-propen-1-yl) pyrrolidine-5-carboxylic acid t-butyl ester (0.161 g, 0.421 mmol) and p-toluenesulfonic acid monohydrate (0.080 g, 0.421 mmol) in 10 mL of dichloromethane was stirred at rt for 1 h. The solvent was evaporated in vacuo to provide the desired product (yield : 0.230 g, 99%) as a white powder. rH NMR (CDCl3) 8 9.2 (bs, 1 H), 9.35 (bs, 1H), 7.79 (d, J=6.4 Hz, 2H), 7.15 (d, J=8.4 Hz, 2H), 6.8 (bd, J=9.9 Hz, 1H), 5.6-5.4 (m, 2H), 4.50 (t, J=9.5 Hz, 1 H), 4.22 (bs, 1 H), 4.10 (bs, 1 H), 3.4-3.3 (m, 1 H), 3.24 (s, 3H), 2.46 (m, 1 H), 2.34 (s, 3H), 1.94 (s, 3H), 1.86 (m, 1H), 1.64 (s, 3H), 1.57 (d, J=5.1 Hz, 3H), 1.52-1.40 (m, 2H), 1.46 (s, 9H), 1.38 (s, 3H), 1.3-1.2 (m, 1H), 0.84 (t, J = 6.8 Hz, 3H).

MS: (M+H) + = 383, (M-H)-= 381.

Anal. Calcd for C28H46N207S : C, 60.62; H, 8.36 ; N, 5.05; S, 5.78. Found: C, 60.64; H, 8.14; N, 4.96; S, 5.70.

Example 273 (- ?- (2R. 3S. 5R, 1'R. 2'S)-2-f 1-acetamido-2-methoxy-2-methyl) penyl-3- (cis-propen- 1-vl) pyrrolidine-5-carboxylic Acid A solution of (-)- (2R, 3S, 5R, 1'R, 2'S)-2- (1-acetamido-2-methoxy-2- methyl) pentyl-3- (cis-propen-1-yl) pyrrolidine-5-carboxylic acid ethyl ester (0.528 g, 1.49 mmol) and lithium hydroxide (72 mg, 3.0 mmol) in methanol (75 mL) and water (25 mL) was stirred at rt for 6 h. Following the addition of 100 mL of 0.1 N HCI the mixture was evaporated in vacuo to provide a white solid. This material was dissolved in water (25 mL) and adsorbed on 15 g of DOWEX 50WX8-400 strongly acidic ion-exchange resin (resin had been prepared by washing with water followed by 0.5 N ammonium hydroxide followed by 1.0 N hydrochloric acid followed by water). After washing the resin with water (250 mL), the desired product was eluted using 0.5 N ammonium hydroxide (250 mL) to provide the desired product (yield : 0.460 g, 90%) as a white solid. [a] =-54. 6 ° in water at rt.

'H NMR (D2O) 8 5.61 (dq, J=8.9,7.2 Hz, 1H), 5.31 (m, 1H), 4.35 (d, J=10.2 Hz, 1 H), 4.10 (t, J=8.9 Hz, 1H), 3.59 (t, J=10. 0 Hz, 1H), 3.26 (s, 3H), 3.14 (quintet, J=8.5 Hz, 1H), 2.54 (dt, J=13. 6, 7.8 Hz, 1 H), 2.16 (m, 1H), 1.95 (s, 3H), 1.75-1.6 (m, 1 H), 1.57 (dd, J=6.8,1.3 Hz, 3H), 1.40 (m, 1H), 1.35-1.25 (m, 2H), 1.24 (s, 3H), 0.85 (t, J=7.0 Hz, 3H), 0.78 (m, 1H).

MS (M+H) + = 327, (M+Na)+ = 349, (M-H)- = 325, (2M-H)-= 651.

Example 274 ()- (2R, 3S. 5R, 1'R, 2'S)-2- 1-acetamido-2-hydroxv) butyl-3- (cis-propen-1-<BR> <BR> <BR> <BR> <BR> <BR> yl) pyrrolidine-5-carboxylic Acid Isopropyl Ester 'H NMR (CDC13, 500 MHz) 5 5.50-5.45 (m, 1H), 5.35 (d, 1H, J=7 Hz), 5.30-5.25 (m, 1H), 5.10-5.0 (m, 1H), 3.80-3.75 (m, 2H), 3.62-3.57 (m, 1H), 3.17- 3.12 (m, 1H), 3.10-3.02 (m, 1H), 2.42-2.37 (m, 1H), 1.93 (s, 3H), 1.61 (d, 3H, J=5 Hz), 1.52-1.42 (m, 3H), 1.28-1.20 (m, 8H), 0.98 (t, 3H, J=7 Hz) Example 275 (~)-(2R,3S, 5R. 1'R. 2'S)-2-(1-acetamido-2-hydroxy)butyl-3-(cis-propen-1- yl) pyrrolidine-5-carboxylic Acid Isobutvl Ester 1H NMR (DMSO-d6, 500 MHz) 5 7.49 (d, 1H, J=10 Hz), 5.32-5.24 (m, 2H), 3.92-3.80 (m, 2H), 3.75 (t, 1H, J=7.7 Hz), 3.48-3.32 (m, 6H), 3.10-3.04 (m, 1H), 2.90-2.83 (m, 1H), 2.45 (s, 1H), 2.29-2.2.22 (m, 1H), 2.19 (s, 1H), 1.75 (s, 2H), 1.52 (d, 2H, J=5 Hz), 1.06 (s, 3H), 0.90 (d, 6H, J=5 Hz) Example 276 (~)-(2R,3S,5R,1'R,2'S)-2-(1-acetamido-2-hydroxy)butyl-3-(cis -propen-1- yl)pyrrolidine-5-carboxylic Acid tert-Butyl Ester 1H NMR (DMSO-d6, 500 MHz) 8 8.95 (s, br,-1 H), 7.88 (d, 1H, J=9.5 Hz), 5.50-5.43 (m, 1 H), 5.35-5.24 (m, 2H), 4.30-4.25 (m, 1 H), 4.03-3.95 (m, 1 H), 3.75- 3.68 (m, 1 H), 2.42-2.33 (m, 1 H), 1.82 (s, 3H), 1.60 (d, 3H, J=6 Hz), 1.58 (d, 3H, J=6 Hz), 1.45 (s, 9H), 1.30-1.22 (m, 2H), 0.85 (t, 3H, J=7.5 Hz) Example 277 ()- (2R, 3S, 5R, 1'R. 2'S)-2- 1-acetamido-2-hydroxy) butyl-3- (cis-propen-1- yl) pyrrolidine-5-carboxvlic Acid Benzyl Ester 1H NMR (DMSO-d6, 500 MHz) 8 7. 49 (d, 1H, J=9.5 Hz), 7.40-7.30 (m, 5H), 5.32-5.24 (m, 2H), 5.19-5.10 (m, 3H), 3.70 (t, 1H), J=7 Hz), 3.62-3.55 (m, 2H), 3.44-3.40 (m, 1H), 3.10-3.05 (t, 1H, J=7 Hz), 2.90-2.82 (m, 1H), 2.30-2.21 (m, 1H), 1.75 (s, 3H), 1.53 (d, 3H, J=6 Hz), 1.45-1.39 (m, 2H), 0.80 (t, 3H, J=7.5 Hz) Example 278 (~)-(2R,3S,5R,1'R,2'S)-2-(1-acetamido-2-hydroxy)butyl-3-(cis -propen-1- yl)pyrrolidine-5-carboxylic Acid Butyl ESter 1 H NMR (DMSO-d6, 500 MHz) 5 9.2 (s, br, 1H), 7.89 (d, 1H, J=9.5 Hz), 7.48 (d, 2H, J=8 Hz), 7.10 (d, 2H, J=8 Hz), 5.50-5.42 (m, 1H), 5.35-5.25 (m, 1H), 4.42-4.37 (m, 1H), 4.21-4.13 (m, 2H), 4.07-4.00 (m, 1H), 3.75-3.69 (m, 1H), 3.47- 3.40 (m, 1H), 3.20-3.10 (m, 1H), 2.47-2.40 (m, 1H), 2.27 (s, 3H), 1.81 (s, 3H), 1.68-1.57 (m, 4H), 1.38-1.22 (m, 5H), 0.92-0.81 (m, 5H).

Example 279 (~)-(2R,3S,5R,1'R,2'S)-2-(1-acetamido-2-hydroxy)butyl-3-(cis -propen-1- yl)pyrrolidine-5-carboxylic Acid Pentyl Ester 1 H NMR (DMSO-d6, 500 MHz) 8 7.50 (d, 1H, J=9.5 Hz), 5.35-5.23 (m, 2H), 4.12-4.00 (m, 2H), 3.30 (s, 3H), 3.72 (t, 1H, J=5.0 Hz), 3.60-3.53 (m, 1H), 3.43-3.40 (m, 1H), 3.10-3.05 (m, 1 H), 3. 90-3.83 (m, 1H), 2.27-2.20 (m, 1H), 1.50- 1.35 (m, 3H), 1.75 (s, 3H), 1.60-1.50 (m, 4H), 1.32-1.15 (m, 5H), 0.9-0.8 (m, 5H).

Example 280 (~)-(2R,3S,5R,1'R,2'S)-2-(1-acetamido-2-hydroxy)butyl-3-(cis -propen-1- <BR> <BR> <BR> vypyrrolidine-5-carboxylic Acid (2-Methyl) benzyl Ester p-Toluenesulfonic Acid Salt 1 H NMR (DMSO-d6, 500 MHz) 8 9.05 (d, br 1H), 7.90 (d, 1H, J=9.5 Hz), 7.47 (d, 2H, J=8.0 Hz), 7.39-7.20 (m, 4H), 7.10 (d, 2H, J=8.0 Hz), 5.50-5.42 (m, 1 H), 5.29-5. 20 (m, 2H), 4.05-4.00 (m, 1 H), 3.75-3.70 (m, 1 H), 2.45-2.40 (m, 1 H), 2.22 (s, 2H), 2.29 (s, 3H), 1.81 (s, 3H), 1.67-1.50 (m, 5H), 1.30-1.22 (m, 4H), 0.88-0.82 (t, 3H, J=7.5 Hz) Example 281 ()- (2R, 3S, 5R, 1'R, 2'S)-2-(1-acetamido-2-methoxy-2-methyl)pentyl-3-(cis-propen- 1-v)rrrolidine-5-carboxylic Acid t-Amyl Ester A mixture of 0.054 g (0.45 mmol, 0.033 mL) thionyl chloride and 0.64 g 2,2-dimethyl-1-propanol in 1 mL anhydrous toluene was stirred at rt for 15 min.

To this mixture was added in one portion 0.011 g (0.0227 mmol) ()- (2R, 3S, 5R, 1'R, 2'S)-2-(1-acetamido-2-methoxy-2-methyl)pentyl-3-(cis-propen- 1- yl) pyrrolidine-5-carboxylic acid trifluoroacetic acid salt. The mixture was stirred at rt for 18 h and then the solvent was evaporated in vacuo. The residue was purified by column chromatography on silica gel using 98: 2: 0.1 dichloromethane/methanol/ammonium hydroxide to give the title compound (6.5 mg, 66% yield) as a colorless oil.

1H NMR (CDC13) 8 5.5-5.25 (m, 3H), 4.17 (dd, J=10. 2,7.1 Hz, 1H), 3.89 (t, J=7.8 Hz, 1H), 3.86 (d, J=10.5 Hz, 1H), 3.75 (d, J=10.5 Hz, 1H), 3.31 (t, J=7.7Hz, 1H), 3.17 (s, 3H), 2.98 (quintet, J=8.3Hz, 1H), 2.33 (dt, J=12. 9,7.8 Hz, 1H), 1.94 (s, 3H), 1.61 (dd, J=6.6,1.3 Hz, 3H), 1.65-1.30 (m, 5H), 1.12 (s, 3H), 0.93 (s, 9H), 0.90 (t, J=7.1 Hz, 3H).

MS: (M+H) +=397, (M+Na) +=419, (M-H)-=395.

Example 282 ()- (2R. 3S, 5R. 1'R. 2'S)-2- (1-acetamido-2-methoxv-2-methvl) penyl-3- (cis-propen- 1-yl)Yrrolidine-5-carboxylic acid Isobutyl Ester A mixture of 0.0714 g (0.60 mmol, 0.044 mL) thionyl chloride and 2 mL 2- methyl-1-propanol was stirred at rt for 15 min. To this mixture was added a solution of 0.0132 g (0.030 mmol) ()- (2R, 3S, 5R, 1'R, 2'S)-2- (1-acetamido-2- methoxy-2-methyl) pentyl-3-(cis-propen-1-yl) pyrrolid ine-5-carboxylic acid trifluoroacetic acid salt in 1 mL of 2-methyl-1-propanol. The mixture was stirred at rt for 48 h and then the solvent was evaporated in vacuo. The residue was purified by column chromatography on silica gel using 98: 2: 0.1 dichloromethane/methanol/ammonium hydroxide to give the title compound (6.5 mg, 57% yield) as a colorless oil.

1H NMR (CDCl3) 5 5.5-5.25 (m, 3H), 4.16 (dd, J=10. 4, 7.3 Hz, 1H), 3.95- 3.8 (m, 3H), 3.30 (t, J=7.3 Hz, 1H), 3.17 (s, 3H), 2.97 (quintet, J=8.3Hz, 1H), 2.32 (dt, J=12. 9, 7.8 Hz, 1H), 1.95 (s, 3H), 1.93 (m, 1H), 1.61 (dd, J=6.6,1.5 Hz, 3H), 1.6-1.2 (m, 5H), 1.11 (s, 3H), 0.92 (d, J=6.4 Hz, 6H), 0.90 (t, J=7.0 Hz, 3H).

Example 283 ()- (2R, 3S. 5R, 1'R, 2'R)-2-(1-acetamido-2-hydroxy-2-ethenyl)pentyl-3-(cis-propen - 1-yl)pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt.

'H NMR (DMSO-d6) 8 7.58 (d, J=10. 4 Hz, 1H), 6.02 (dd, J = 17.1,10.4 Hz, 1 H), 5.5-5.4 (m, 2H), 5.30 (dd, J = 11.0,1.8 Hz, 1 H), 5.20 (m, 1 H), 4.35-4.30 (m, 2H), 3.38 (t, J=9.2 Hz, 1H), 3.07 (quintet, J=8.8 Hz, 1H), 2.42 (dt, J = 13.4,7.9 Hz, 1H), 1.80 (s, 3H), 1.55 (m, 1H), 1.51 (dd, J=6.7,1.8 Hz, 3H), 1.43-1.30 (m, 4H), 1.22 (m, 1 H), 0.81 (t, J = 7.0 Hz, 3H).

MS: (M+H)+ = 325, (M+Na) + = 347, (2M+H) + = 649, (M-H)-= 323, (2M-H)-= 647.

Example 284 ()- (2R, 3S, 5R. 1'R. 2'R)-2-(1-acetamido-2-methoxy-2-ethenyl)pentyl-3-(cis- propen-1-yl)pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt.

'H NMR (DMSO-d6) 8 7.58 (d, J=10. 4 Hz, 1H), 6.02 (dd, J = 17.1,10.4 Hz, 1 H), 5.5-5.4 (m, 2H), 5.30 (dd, J = 11.0,1.8 Hz, 1 H), 5.20 (m, 1 H), 4.35-4.30 (m, 2H), 3.38 (t, J=9.2 Hz, 1H), 3.07 (quintet, J=8.8 Hz, 1 H), 2.42 (dt, J = 13.4,7.9 Hz, 1 H), 1.80 (s, 3H), 1.55 (m, 1H), 1.51 (dd, J=6.7,1.8 Hz, 3H), 1.43-1.30 (m, 4H), 1.22 (m, 1H), 0.81 (t, J = 7.0 Hz, 3H).

Example 285 285A (~)-(2R,3S,5R,1'R)-1-t-Butoxycarbonyl-2-(1-acetamido-2-triis opropyl- silyloxy)ethyl-3-ethoxyethyloxymethylpyrrolidine-5-carboxyli c acid t-butvl ester ()- (2R, 3R, 5R, 1'R)-1-t-Butoxycarbonyl 2- (1-acetamido-2-triisopropyl- silyloxy) ethyl-3-hydroxymethylpyrrolidine-5-carboxylic acid t-butyl ester (1.06 g, 1.90 mmol) in 40 ml of dichloromethane was reacted with pyridinium p- toluenesulfonate (95 mg, 0.38 mmol) and ethyl vinyl ether (0.36 ml, 3.80 mmol) 1 hour at room temperature. The reaction was quenched with saturated aqueous NaHCO3, extracted with dichloromethane (5 x 30 ml). The combined organic layers were dried over Na2S04 and concentrated to give the crude title compound (yield : 1.2 g, 100%).

MS: (M+H) += 631. 285B (_)-(2R, 3R, 5R. 1'R)-1-t-Butoxycarbonyl-2-(1-acetamido-2-hydroxy) ethyl-3- ethoxyethyloxvmethylpyrrolidine-5-carboxylic acid t-butyl ester The product of Example 285A (1.2 g, 1.90 mmol) in THF (60 ml) was reacted with tetrabutyl ammonium fluoride (1 M in THF) (1.90 ml, 1.90 mmol) for 60 minutes at room temperature. Water was added followed by extraction using dichloromethane (5x40 ml). This organic layers were dried over Na2SO4, filtered and concentrated in vacuo. The residue was purified by column chromatography on silica gel using 10% methanol in dichloromethane to provide the title compound as a colorless oil (yield : 791.6 mg, 87.7%).

1H NMR (CDC13) : 81. 18 (td, 3H), 1.26 (dd, 3H), 1.44 (s, 9H), 1.47 (s, 9H), 1.85 (m, 1 H), 2.00 (s, 3H), 2.39 (m, 1 H), 2.55 (m, 1 H), 3.27-3.67 (m, 6H), 3.83 (m, 1 H), 3.92 (d, 1H), 4.12 (dt, 1H), 4.26 (m, 1H), 4.64 (m, 1H), 6.36 (br d, 1 H).

MS: (M+H)+= 475.

285C ()- (2R. 3R, 5R, 1'R)-1-t-Butoxycarbonvl-2- (1-acetamido-1-formvl) methyl-3-<BR> <BR> <BR> <BR> ethoxyethyloxymethylpyrrolidine-5-carboxylic Acid t-Butyl Ester The product of Example 285B (790.0 mg, 1.66 mmol) was reacted with Dess-Martin Periodinane (850 mg, 2.0 mmol) in dichloromethane (40 ml) at room temperature for 2 hours. The reaction was quenched with 1 M aqueous sodium thiosulfate (40 ml) and extracted with dichloromethane (5 x 40 ml). The organic layers were dried over sodium sulfate, filtered, and concentrated in vacuo. The residue was purified by column chromatography on silica gel using ethyl acetate to provide the title compound as a white foamy solid (yield : 707.1 mg, 89.9%). aH NMR (CDC13) : 8 1. 18 (td. 3H), 1.27 (dd, 3H), 1.42 (s, 9H), 1.47 (s, 9H), 1.71 (m, 1H), 2.08 (s, 3H), 3.34-3.67 (m, 4H), 3. 92 (dd, 1H), 4.18 (dd, 1 H), 4.65 (m, 1H), 4.89 (ddd, 1H), 7.15 (m, 1H), 9.46 (s, 1H).

MS: (M+H) += 473.

285D ()- (2R. 3R. 5R. 1'R. 2'R and 2'S !-1-t-Butoxvcarbonyl-2-(1-acetamido-2- <BR> <BR> <BR> hvdroxv) butvl-3-ethoxvethyloxymethilpyrrolidine-5-carboxylic Acid t-Butyl Ester The product of Example 285C (700 mg, 1.48 mmol) in THF (15 ml) was added dropwise to a solution of ethylmagnesium chloride (3.0M in ether) (3.0 ml, 8.9 mmol) in THF (15 ml) at room temperature and reacted for 30 minutes. The reaction was quenched with saturated aqueous ammonium chloride (15 ml) followed by extraction with dichloromethane (5 x 20 ml). The organic layer was dried over sodium sulfate, filtered and concentrated in vacuo, to provide the crude title compound (yield : 756.2 mg, 100%).

MS: (M+H) = 503.

285E (~)-(2R,3S,5R,1'R)-1-t-Butoxycarbonyl-2-(1-acetamido-2-oxo)b utyl-3- ethoxyethyloxymethylpvrrolidine-5-carboxylic Acid t-Butvl Ester The title compound was prepared according to the method described in Example 285C, substituting the product of Example. 285D in place of the product of Example 285B (yield : 574.2 mg, 77.4%). rH NMR (CDC13) : # 1. 04 (t, J=7.2 Hz, 3H), 1.18 (td, J=6.9 3.6 Hz, 3H), 1.24 (dd, J=5.4,1.2 Hz, 3H), 1.45 (s, 9H), 1.46 (s, 9H), 1.65 (m, 1H), 2.04 (s, 3H), 2.28 (m, 1 H), 2.42 (m, 1 H), 2.63 (qd, J=7.2,1.5 Hz, 2H), 3.25 (dd, J= 9.3, 6.3 Hz, 1 H), 3.36-3.46 (m, 2H), 3.54-3. 61 (m, 2H), 4.09-4.18 (m, 2H), 4.61 (t, J=5.1 Hz, 1H), 5.23 (br t, J=7.2 Hz, 1H).

MS: (M+H) += 501.

285F (i)-(2R, 3R, 5R. 1'R. 2'R !-1-t-Butoxycarbonvl-2-(1-acetamido-2-hydroXv-2- vinyl) butvl-3-ethoxyethyloxymethvlpyrrolidine-5-carboxylic Acid t-Butyl Ester The title compound was prepared according to the method described in Example 285D, substituting compound the product of Example 285E in place of the product of Example 285C and substituting vinyl magnesium bromide in place of ethyl magnesium bromide (yield : 272.1 mg, 45.2%).

MS: (M+H) += 529.

285G (~)-(2R,3S,5R,1'R,2'R)-1-t-Butoxycarbonyl-2-(1-acetamido-2-m ethoxy-2- vinvl) butyl-3-ethoxyethyloxvmethylpyrrolidine-5-carboxylic Acid t-Butyl Ester The product of Example 285F (200 mg, 0.38 mmol) in THF (6 ml) was reacted with sodium bis (trimethylsilyl) amide (1.0M in THF, 0.76 ml, 0.76 mmol) at -78°C for 45 minutes then at 0°C for 15 minutes. Methyl iodide (2.4 ml, 38.0 mmol) was added to the reaction at 0°C. Stirring was continued at 0°C for 30 minutes then at room temperature for 2 hours. The reaction was quenched with saturated aqueous ammonium chloride (10 ml) followed by extraction using dichloromethane (5 x 20 ml). The organic layer was dried over sodium sulfate, filtered and concentrated in vacuo. The residue was purified by column chromatography on silica gel using 5% methanol in dichloromethane to provide the title compound (yield : 202.0 mg, 98.4%).

MS: (M+H) += 543.

285H(i)-(2R. 3R. 5R. 1'R)-1-t-Butoxycarbonvl-2-(1-acetamido-2-methoxy-2- ethyl) 1)butyl-3-ethoxyethyloxymethylpyrrolidine-5-carboxylic Acid t-Butyl Ester The product of Example 285F (200 mg, 0.369 mmol) and 10% Pd-C (100 mg) were stirred under 1 atmosphere of hydrogen in ethyl acetate (50 ml) for 2 hours. The reaction was filtered and concentrated, to give the crude title compound (yield : 198.1 mg, 98.7%).

MS: (M+H) += 545.

2851 (i)-(2R. 3R. 5R. 1'R)-1-t-Butoxycarbonyl-2-(1-acetamido-2-methoxv-2- ethyl) butyl-3-hydroxymethylpyrrolidine-5-carboxylic Acid t-Butyl Ester The product of Example 285H (196.0 mg, 0.36 mmol) was dissolved in THF (20ml) and treated with 0.2N hydrochloric acid (20 ml) at room temperature for 2 hour. The reaction was quenched with saturated aqueous sodium bicarbonate followed by extraction using dichloromethane (5 x 20 ml). The organic layer was dried over sodium sulfate, filtered and concentrated in vacuo.

The residue was purified by column chromatography on silica gel using 5% methanol in dichloromethane to provide the title compound (yield : 103.2 mg, 60.7%).

'H NMR (CDC13) (mixture of two rotamers) 8 : 0.87,0.89 (two t, J=7.5 Hz, 3H), 1.06,1.26 (two t, J=7.5 Hz, 3H), 1.45,1.46 (two s, 9H), 1.52,1.55 (two s, 9H), 1.73-1.94 (m, 5H), 1.98,1.99 (two s, 3H), 2.29-2.42 (m, 1 H), 2.66-2.91 (m, 1 H), 3.27, 3.28 (two s, 3H), 3.51-3.72 (m, 2H), 4.00-4.11 (m, 1H), 4.18- 4.29 (m, 1 H), 4.67,4.70 (two dd, 1 H), 5.76,5.81 (two br d, 1 H).

MS: (M+H) += 473.

285J()- (2R. 3R, 5R. 1'R)-1-t-Butoxycarbonyl-2- (1-acetamido-2-methoxy-2-<BR> <BR> <BR> <BR> ethvl) butvl-3-formylpyrrolidine-5-carboxylic Acid t-Butrl Ester The title compound was prepared according to the method described in Example 285C, substituting the product of Example 2851 in place of the product of Example 285B (yield : 40.1 mg, 80.5%).

MS: (M+H) += 471.

285K(i)-(2R. 3S, 5R. 1'R !-1-t-Butoxycarbonyl-2-(1-acetamido-2-methoxy-2-<BR> <BR> <BR> <BR> <BR> ethVl) butVl-3-(cis-nropen-1-vl) pyrrolidine-5-carboXviic Acid t-Butyl Ester A suspension of ethyl triphenylphosphonium bromide (180 mg, 0.48 mmol) in anhydrous toluene (2 ml) was reacted with potassium t-butoxide (1.0 M in THF, 0.32 mmol) at room temperature for 14 hours. The product of Example 285J (38 mg, 0.081 mmol) in toluene (2 ml) was added dropwise to the above mixture and stirred for 1 hour. The reaction was quenched with saturated aqueous ammonium chloride and extracted with dichloromethane (5 x 10 ml).

The organic layer was washed with brine, dried over Na2SO4, filtered and concentrated in vacuo. The residue was purified by chromatography on silica gel using 50% ethyl acetate/hexanes to provide the title compound (yield : 20.1 mg, 53.8%).

1H NMR (CDCl3) (mixture of rotamers) 8 : 0.84 0.91 (3H), 0.97-1.11 (3H), 1.42-1.47 (9H), 1.54-1.55 (9H), 1.60-1.64 (3H), 1.66 (1 H), 1.72-1.80 (4H), 1.97-1.99 (3H), 2.42-2.58 (1 H), 3.23-3.25 (3H), 3.50-3.73 (1 H), 4.03-4.18 (2H), 4.67-4.73 (1 H), 5.29-5.38 (1 H), 5.57-5.71 (1 H), 5.83-6.02 (1 H).

MS: (M+H) += 483.

285L ()- (2R, 3S. 5R. 1'R)- 2- (1-acetamido-2-methoxy-2-ethvl) buyl-3- (cis- propen-1-yl) pyrrolidine-5-carboxvlic Acid Trifluoroacetic Acid Salt The product of Example 285K (17.0 mg, 0.035 mmol) was reacted with trifluoroacetic acid (0.8 mL) in dichloromethane (0.2 mL) at room temperature for 4 hours. The reaction was concentrated in vacuo and triturated with dichloromethane (4 x 0.2 ml) to provide the title compound (yield: 15.6 mg, 100%) as an off white solid.

'H NMR (DMSO-d6) 8 0.81 (t, 3H), 0.96 (t, 3H), 1.29 (m, 1H), 1.53 (dd, 3H), 1.55-1.65 (m, 3H), 1.70 (m, 1H), 1.82 (s, 3H), 2.45 (m, 1H), 3.10 (m, 1H), 3.17 (s, 3H), 3.72 (m, 1H), 4.24 (m, 1H), 4.39 (t, 1H), 5.29 (m, 1H), 5.45 (m, 1H), 7.58 (d, 1 H), 8.50 (br s, 1 H), 9.03 (br s, 1 H).

. Example 286 (~)-(2R,3S,5R,1'R,2'S)-2-(1-Acetamido-2-methyl-2,3-dimethoxy )ethyl-3- (cis-propen-1-vl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 286A1()- (2R, 3S, 5R. 1'R. 2'R)-1-t-Butoxycarbonyl-2-f1-acetamido-3-<BR> <BR> <BR> <BR> <BR> <BR> ethoxyethyloxy-2-hydroxv) propyl-3- (cis-propen-1-yl)-pyrrolidine-5-carboxvlic Acid t-Butyl Ester and 286A2 ()- (2R, 3S. 5R. 1'R. 2'S)-1-t-Butoxycarbonyl-2-(1- <BR> <BR> <BR> acetamido-3-ethoxvethv!oxv-2-hvdroxv)propv!-3-(c/s-propen-1- yt)-pvrro!idine-5- carboxvlic Acid t-Butyl Ester (Ethoxyethyloxymethyl) tributylstannane (prepared according to the procedure of W. Clark Still, J. Am. Chem. Soc., 100,1481 (1978)) (575 mg, 1.46 mmol) in THF (4ml) was reacted with n-butyllithium (1.6M, 0.6 ml, 0.96 mmol) at- 78°C for 15 minutes followed by the product of Example 41A (70 mg, 0.17 mmol) in THF (2 ml). The mixture was stirred at-78°C for an additional 30 minutes, quenched with saturated aqueous ammonium chloride (3 ml) followed by extraction using dichloromethane (5 x 10 ml). The organic layer was dried over magnesium sulfate, filtered and concentrated in vacuo. The residue was separated by column chromatography on silica gel using 5% methanol in dichloromethane to provide the title compounds 286A1 (yield : 25.1 mg, 28.7%) and 286A2 (yield : 30.9 mg, 35.3%) as white foamy solids.

286A1 : 1H NMR (CDC13) : # 1. 20 (dt, J=7.2,2.4Hz, 3H), 1.30 (dd, J=5.4, 3.9 Hz, 3H), 1.44 (s, 9H), 1.47 (s, 9H), 1.61 (m, 1 H), 1.65 (dd, J=6.6,1.5 Hz, 3H), 2.04 (s, 3H), 2.62 (m, 1H), 3.38-3.70 (m, 8H), 4.23 (dd, J=9.3,5.1 Hz, 1H), 4.67 (qd, J=5.1,2.4 Hz, 1 H), 4.37-4.48 (m, 2H), 7.77 (br t, J=9.9 Hz, 1 H).

286A2 : 1H NMR (CDC13) : 81. 19 (dt, J=7.2,2.4Hz, 3H), 1.29 (t, J=5.1 Hz, 3H), 1.44 (s, 9H), 1.46 (s, 9H), 1.54 (dd, 3H), 1.64 (m, 1H), 2.00 (s, 3H), 2.66 (m, 1H), 3.13 (m, 1H), 3.41-3.49 (m, 2H), 3.59-3.69 (m, 2H), 3.75 (d, J=9.9 Hz, 1H), 3.89 (td, J=10. 2, 3.0 Hz, 1H), 4.15 (ddd, J= 10.2,3.0,1.2 Hz, 1H), 4.65 (dd, J=9.6,5.1 Hz, 1H), 4.72 (dd, J=6.6,4.8 Hz, 1H), 5.35 (m, 1H), 5.60 (br t, J=10. 2 Hz, 1H), 5.99 (brd, J=10. 2 Hz, 1H). 286B (ffi)-(2 (2R. 3S, 5R. 1'R)-1-t-Butoxycarbonyl-2-(1-acetamido-3-ethoxyethyloxy- 2-oxo)propyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic Acid t-Butyl Ester The products of Example 286A (298.0 mg, 0.58 mmol) were reacted with Dess-Martin Periodinane (368.4 mg, 0.87 mmol) in dichloromethane (20 ml) at room temperature for 2 hours. The reaction was quenched with 1 M aqueous sodium thiosulfate (20 ml) and extracted with dichloromethane (5 x 20 ml). The organic layer was dried over sodium sulfate, filtered, and concentrated in vacuo.

The residue was purified by column chromatography on silica gel using ethyl acetate to provide the title compound as a white foamy solid (yield : 254.2 mg, 85.6%).

'H NMR (CDC13) : 8 1.17 (t, 3H), 1.31 (dd, 3H), 1.41 (s, 9H), 1.46 (s, 9H), 1.56 (m, 1 H), 1.64 (dt, 3H), 2.06 (s, 3H), 2.45 (m, 1H), 3.47 (m, 2H), 3.62 (m, 2H), 4.12-4.48 (m, 3H), 4.75 (m, 1 H), 4.96 (ddd, 1 H), 5.29 (m, 1 H), 5.58 (m, 1 H), 7.83 (br q, 1 H).

MS: (M+H)+= 513.

286C (i)-(2R. 3S. 5R. 1'R. 2'R !-1-t-Butoxvcarbonyl-2-(1-acetamido-3- ethoxyethyloxv-2-hydroxv-2-methvl) propvl-3- (cis-propen-1-yl)-pvrrolidine-5- carboxylic Acid t-Butvl Ester The product of Example 286B (60.0 mg, 0.117 mmol) in THF (2 ml) was added dropwise to a solution of methylmagnesium bromide (3.0M in ether) (0.2 mL, 0.59 mmol) in THF (2 ml) at room temperature and reacted for 60 minutes.

The reaction was quenched with saturated aqueous ammonium chloride (2 ml) followed by extraction using dichloromethane (5 x 10 ml). The organic layer was dried over sodium sulfate, filtered and concentrated in vacuo, to provide the title compound (yield : 56.0 mg, 90.5%).

MS: (M+H) += 529.

286D (+)-(2R. 3S, 5R. 1'R. 2'R)-1-t-Butoxycarbonyl-2-(1-acetamido-2. 3-dihydroxy- 2-methyl) l)propyl-3-(cis-propen-1-yl)-pyrrolidine-5-carboxylic Acid t-Butyl Ester The product of Example 286C (55.0 mg, 0.104 mmol) was dissolved in THF (5 ml) and reacted with 0.1 N hydrochloric acid (5 ml) at room temperature for 0.5 hour. The reaction was quenched with saturated aqueous sodium bicarbonate followed by extraction using dichloromethane (5 x 10 ml). The organic layer was dried over sodium sulfate, filtered and concentrated in vacuo.

The residue was purified by column chromatography on silica gel using 5% to 10% methanol in dichloromethane to provide the title compound (yield : 29.1 mg, 61.3%).

1H NMR (CDC13) 8 : 1.17 (s, 3H), 1.43 (s, 9H), 1.46 (s, 9H), 1.63 (dd, 3H), 1.65 (m, 1H), 2.06 (s, 3H), 2.64 (s, 1H), 2.83 (m, 1 H), 3.16 (m, 1H), 3.33 (m, 1 H), 3.44 (m, 1 H), 4.01 (br s, 1 H), 4.23 (m, 2H), 4.48 (m, 1 H), 5.37-5.55 (m, 2H), 8.05 (br d, 1 H).

286E (i)-(2R. 3S. 5R. 1'R, 2'R)-1-t-Butoxycarbonvl-2-(1-acetamido-2, 3-dimethoxy- 2-methyl) propyl-3- (cis-propen-1-yl-pvrrolidine-5-carboxvlic Acid t-Butyl Ester The product of Example 286D (28.0 mg, 0.061 mmol) in THF (2 ml) was reacted with sodium bis (trimethylsilyl) amide (1.0M in THF, 184 pI, 0.184 mmol) at -78°C for 45 minutes and then 0°C for 15 minutes. Methyl iodide (190 pI, 3.05 mmol) was added to the mixture at 0°C and stirred at 0°C for 30 minuntes then at room temperature for 1 h. The reaction was quenched with saturated aqueous ammonium chloride (2 ml) followed by extraction using dichloromethane (5 x 10 ml). The organic layer was dried over sodium sulfate, filtered and concentrated in vacuo. The residue was purified by column chromatography on silica gel using 5% methanol in dichloromethane to provide the title compound (yield : 28.2 mg, 94.5%).

1H NMR (CDCb) (the major rotamer) 8 : 1.28 (s, 3H), 1.44 (s, 9H), 1.46 (s, 9H), 1.63 (dd, J=7.2,1.8Hz, 3H), 1.70 (m, 1 H), 2.01 (s, 3H), 2.49 (m, 1 H), 3.24 (s, 3H), 3.25 (d, 1H), 3.35 (s, 3H), 3.38 (m, 2H), 3.87 (br s, 1H), 4.11 (dd, J=10.5, 1.5 Hz, 1H), 4.80 (m, 1H), 5.37 (m, 1H), 5.63 (td, J=10. 2,2.1 Hz, 1 H), 6.46 (br d, J=8.7 Hz, 1 H).

286F (~)-(2R,3S, 5R. 1'R. 2'R)-2- (1-acetamido-2. 3-dimethoxy-2-methyl) propyl-3- (cis-propen-1-vl)-nvrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt The product of Example 286E (10.2 mg, 0.021 mmol) was reacted with trifluoroacetic acid (0.8 ml) in dichloromethane (0.2 ml) at room temperature for 4 hours. The reaction was concentrated in vacuo and triturated with dichloromethane (4 x 0.2 ml) to provide the title compound (yield : 9.2 mg, 98.9%) as an off white solid.

'H NMR (DMSO-d6) 8 : 1.13 (s, 3H), 1.56 (dd, 3H), 1.63 (m, 1H), 1.84 (s, 3H), 2.45 (dt, 1H), 3.18 (m, 1H), 3.19 (dAB, J=11. 0 Hz, 1H), 3.22 (s, 3H), 3.24 (s, 3H), 3.41 (dAB, J=11. 0 Hz, 1 H), 3.69 (t, 1 H), 4.28 (t, 1 H), 4.46 (t, 1 H), 5.30 (tq, 1 H), 5.46 (dq, 1 H), 7.46 (d, J=10. 0 Hz, 1 H), 8.75 (br s, 2H).

MS: (M+H) += 329.

Example 287 (i)-(2R. 3S. 5R. 1'R, 2'S)-2-(1-acetamido-2-methoxy-2-methvl) butvl-3-(cis-propen-<BR> <BR> 1-yl)-pyrrolidine-5-carboxylic Acid Trifluoroacetic Acid Salt 'H NMR (DMSO-d6) 8 0.75 (t, J=4.5 Hz, 3H), 1.19 (s, 3H), 1.33 (td, J=9.0, 4.5 Hz, 1H), 1.53 (dd, J=4.2,0.9 Hz, 3H), 1.56 (q, J=5.7 Hz, 2H), 1.79 (s, 3H), 2.43 (dt, J=8.1,4.5 Hz, 1H), 3.14 (s, 3H), 3.15 (m, 1H), 3.59 (br m, 1H), 4.30 (br m, 1H), 4.34 (t, J=6.0,0.9 Hz, 1 H), 5.28 (td, J=6.0,0.9 Hz, 1 H), 5.46 (dq, J=6.6, 4.2 Hz, 1 H), 7.59 (d, J=6.0 Hz, 1 H), 8.61 (br s, 1 H), 8.97 (br s, 1 H).

MS: (M+H) += 313.

Example 288 (-)-(2R,3S, 5R. 1'R. 2'S)-2-(1-Acetamido-2-methoxy-2-methyl)pentyl-3-(cis-propen- 1-vppyrrolidine-5-carboxylic Acid Hydrochloride Salt 288A tert-Butyl (2R,3S)-2-((S)-((tert-butyl(dimethyl)silyl)oxy)((4R)-2,2-dim ethyl- 1.3-dioxolan-4-y methyl)-5-oxo-2. 5-dihydro-1 H-pvrrole-1-carboxylate The title compound was prepared according to the procedure described in Tetrahedron Asymmetry, 1996, 1167-1180.

288B tert-Butvl (2R,3S)-2-((S)-((tert-butyl(dimethyl)silyl)oxy)((4R)-2,2-dim ethyl- 1. 3-dioxolan-4-yl)methyl)-5-oxo-3-((1Z)-1-propenyl)-1-pyrrolid inecarboxylate To a solution of copper bromide/dimethyl sulfide (5.9 g, 28.8 mmol) in THF (100 mL) at-78 °C was added 1-propenylmagnesium bromide (0.5M in THF, 113 mL, 56.5 mmol) over 1.5 h followed by trimethylsilyl chloride (2.26 mL, 17.8 mmol) in THF (28 mL). To this solution was added the product of example 288A (2.44 g, 5.71 mmol) in THF (20 mL) at-78 °C, and the reaction mixture was warmed to-22 °C for 2 h. The reaction mixture was quenched with sat. NH4CI and warmed to 25 °C. The mixture was extracted with ethyl acetate, the organic layer was separated, dried, filtered, and the solvent was evaporated. The crude residue was purified by column chromatography on silica gel using 80% hexane/ethyl acetate to give title compound (2.5 g, 93%). rH NMR (CDC13) 8 5.52-5.40 (m, 2H), 4.15-4.00 (m, 3H), 3.92 (d, 1H), 3.74 (dd, 1H), 3.33 (t, 1H), 2.92 (dd, 1 H), 2.13 (dd, 1 H), 2.65 (d, 3H), 1.53 (s, 9H), 1.30 (d, 6H), 0.88 (s, 9H), 0.12 (d, 6H).

MS : (M+H) + = 470, (M+Na) + = 492 288C tert-Butyl (2R,3S,5R)-2((S)-((tert-butyl(dimethyl)silyl)oxy)((4R)-2,2- dimethyl-1. 3-dioxolan4-yl) methyl)-5-cyano-3-((1 Z)-1-oropenyl !-1- pyrrolidinecarboxylate i. To a solution of the product of example 288B (2.84 g, 6.05 mmol) in THF (12 mL) at-78 °C was added diisobutylaluminum hydride (1M in hexane, 10.6 mL, 10.6 mmol). After 0.5 h the reaction was quenched at-78 °C with sat. NH4CI (45 mL), 10% sodium potassium tartrate solution (45 mL), diluted with ethyl acetate, and stirred for 1 h at 25 °C. The organic layer was separated, dried, and the solvent was evaporated to give 2.85 g crude oil used directly for the next step. ii. The above crude product was stirred in methanol (50 mL) with pyridinium p-toluenesulfonate (0.15 g, 0.59 mmol) at 25 °C for 1 h after which the mixture was quenched with brine, extracted with ethyl acetate, dried, filtered, and the solvent was evaporated to give 2.57 g of an oil which was used directly for the next step. iii. To a solution of crude product of example 288C (ii) in dichloromethane (40 mL) at-78 °C was added trimethylsilyl cyanide (2.1 mL, 15.78 mmol) and boron trifluoride-etherate (0.98 mL, 7.76 mmol). After 1 h the reaction was quenched with sat. sodium bicarbonate and warmed to 25 °C and extracted with dichloromethane. The solvents were evaporated and the crude residue was purified by column chromatography on silica gel using 1 % methanol/dichloromethane to give the title compound (2.48 g, 84% over three steps). rH NMR (CDC13) 8 5.8-5.68 (m, 1H), 5.56-5.45 (m, 1H), 4.52-4.47 (dd, 1H), 4.17-4.08 (m, 2H), 4.05-3.65 (m, 3H), 3.4 (m, 1H), 2.65 (m, 1H), 1.97 (d, 1H), 1.66 (dd, 3H), 1.52 (s, 9H), 1.3 (s, 6H), 0.84 (s, 9H), 0.12 (dd, 6H) MS: (M+H) + = 481, (M+Na) + = 503 288Dtert-Butyl (2R,3S,5R)-5-cyano-2-((S)-((4R)-2,2-dimethyl-1,3-dioxolan-4- yl)(hydroxy) methyl)-3-((1 Z)-1-pronenyl)-1-pyrrolid inecarboxylate To a solution of the product of example 288C (2.48 g, 5.16 mmol) in THF (32 mL) at 0 °C was added tetrabutylammonium fluoride (1M in THF, 10 mL, 10 mmol) over 1 h. After 0.5 h the reaction was quenched with sat. NH4CI and ethyl acetate. The organic layer was separated, dried, and the solvents were evaporated. The crude residue was purified by column chromatography on silica gel using 15% ethyl acetate/dichloromethane to give the title compound (1.75 g, 92.5%).

1H NMR (CDC13) 8 5.8-5.52 (m, 2H), 4.48 (dd, 1H), 4.15-4.08 (m, 2H), 3.93 (dd, 1 H), 3.86 (d, 1 H), 3.62-3.45 (m, 2H), 2.63 (ddd, 1 H), 2.07 (d, 1 H), 1.68 (dd, 3H), 1.53 (s, 9H), 0.88 (s, 9H), 1.4 (s, 3H), 1.34 (s, 3H) MS: (M+CI)-= 401; (M+H) + = 367, (M+Na) + = 389 288E tert-Butrl (2R. 3S. 5R)- ((R)-azido ((4S)-2. 2-dimethyl-1. 3-dioxolan-4- yl)methyl)-5-cyano-3-((1Z)-1-propenyl)-1-pyrrolidinecarboxyl ate To a solution of the product of example 288D (1.75 g, 4.78 mmol) in THF (28 mL) at 0 °C was added sequentially, triphenylphosphine (2.25 g, 8.6 mmol), diethyl diazodicarboxylate (1.35 mL, 8.6 mmol), and diphenylphosphoryl azide (1.85 mL, 8.6 mmol). The reaction mixture was allowed to warm to 25 °C over 16 h before being quenched with water (100 mL), 10% citric acid (50 mL), and ethyl acetate. The organic layer was separated, dried, and the solvents were evaporated. The crude residue was purified by column chromatography on silica gel using 12% ethyl acetate/dichloromethane to give the title compound (1.25 g, 67%). rH NMR (CDC13) 8 5.68-5.48 (m, 2H), 4.53 (d, 1H), 4.2-3.97 (m, 3H), 3.83 (t, 1 H), 3.55-3.30 (m, 2H), 2.74 (ddd, 1H), 1.98 (dt, 1 H), 1.68 (dd, 3H), 1.53 (s, 9H), 0.88 (s, 9H), 1.46 (s, 3H), 1.37 (s, 3H) MS : (M+H) + = 392, (M+Na) + = 414 288F tert-Buyl (2R, 3S, 5R)-2-((1 R. 2S)-1-(acetylamino)-2, 3-dihvdroxypropyl)-5-<BR> <BR> <BR> <BR> <BR> cyano-3- ( (1Z-1-propenyl)-1-pyrrolidinecarboxvlate i. To a solution of the product of example 288E (1.25 g, 3.19 mmol) in THF: water (4: 1) (44 mL) was added triphenylphosphine (1 g, 3.84 mmol), and the mixture was heated to 75 °C for 18 h. The solvents were evaporated and the crude amine was azeotroped with benzene (10 mL). ii. The crude amine was dissolved in dichloromethane (30 mL) at 25 °C and combined with triethylamine (3.73 mL, 26.8 mmol), acetic anhydride (1.26 mL, 13.4 mmol), and 4-dimethylaminopyridine (0.044 g, 0.34 mmol). After 1 h, water and dichloromethane were added, and the organic layer was separated and passed through a small pad of silica gel with ethyl acetate to give 2.2 g crude acetamide containing triphenylphosphine and used directly for the next step. iii. The crude N-acetamide (2.2 g) was dissolved in 80% acetic acid (100 mL) at 25 °C for 4 days. The solvents were evaporated, and the crude product was purified by column chromatography on silica gel using 5% methanol/ethyl acetate to give the title compound (0.92 g, 78% over three steps). aH NMR (CDCI3) õ 5.94 (d, 1H), 5.8-5.55 (m, 2H), 4.49 (d, 1 H), 4.46 (dd, 1H), 3.88-3.54 (m, 5H), 3.34 (m, 1H), 3.28 (dd, 1H), 2.65 (m, 1 H), 2.16 (d, 1 H), 2.07 (s, 3H), 1.63 (dd, 3H), 1.56 (s, 9H) MS: (M-H)-= 366, (M+CI)-= 402; (M+H) + = 368, (M+Na) + = 390 288G tert-Buyl (2R, 3S. 5R)-2-((1 R)-1-(acetylamino)-(2R and 2S)-2-<BR> <BR> <BR> <BR> <BR> hydroxypentvl)-5-cyano-3- ((1 Z)-1-propenyl)-1-pyrrolidinecarboxylate i. To a solution of the product of example 288F (0.92 g, 2.51 mmol) in 30% aqueous ethanol (24 mL) at 0 °C was added sodium metaperiodate (1.6 g, 7.48 mmol). The mixture was stirred for 1 h before diluting with ethyl acetate. The organic layer was separated, dried, and the solvents were evaporated and azeotroped with toluene to give 1 g of crude aldehyde which was used directly for the next step. ii. To a solution of propyl magnesium chloride (2M in ether, 3.65 mL, 7.3 mmol) in THF (14 mL) at 0 °C was added the product of example 288G (i) (1g) in THF (10 mL) over 0.5 h. The mixture was stirred for 0.5 h before being quenched with sat. NH4CI, extracted with ethyl acetate, dried, and the solvents were evaporated. The crude residue was purified by column chromatography on silica gel using ethyl acetate to give the title compounds (0.665 g, 70% as a 3.5: 1 mixture of stereoisomers).

Major isomer : 1H NMR (CDCI3) õ 5.98 (d, 1H), 5.77-5.55 (m, 2H), 4.45 (d, 1H), 4.3 (d, 1H), 3.72-3.56 (m, 2H), 3.48 (m, 1H), 3.24 (dd, 1H), 2.7 (m, 1H), 2.14 (d, 1H), 2.03 (s, 3H), 1.62 (dd, 3H), 1.55 (s, 9H), 1.5-1.2 (m, 4H), 0.88 (t, 3H), MS: (M-H)-= 378, (M+CI)-= 414; (M+H) + = 380, (M+Na) + = 402 Minorisomer: 1H NMR (CDCl3) # 6. 7 (d, 1 H), 5.68-5.53 (m, 2H), 4.42 (dd, 1 H), 4.02 (m, 1 H), 3.76 (dd, 1 H), 3.53 (m, 1 H), 3.32 (m, 2H), 2.64 (m, 1 H), 2.07 (d, 1 H), 2.01 (s, 3H), 1.66 (d, 3H), 1.55 (s, 9H), 1.5-1.24 (m, 4H), 0.92 (t, 3H) MS: (M-H)-= 378, (M+CI)-= 414; (M+H) + = 380, (M+Na) + = 402 288H tert-Butyl (2R, 3S. 5R)-2- ((1R)-1-(acetylamino)-2-oxopentyl)-5-cyano-3- ( Z)-1-pronenyl)-1-pyrrolidinecarboxylate To the product alcohols of example 288G (0.665 g, 1.75 mmol) in dichloromethane (18 mL) was added Dess-Martin reagent (1.05 g, 2.47 mmol) at 25 °C. After 1.5 h the reaction mixture was diluted with ether (100 mL) and filtered through Celite. The organic solution was washed with 10% sodium thiosulfate (50 mL), and the solvents were evaporated. The crude residue was purified by column chromatography on silica gel using ethyl ether to give the title compound (0.5 g, 76%). aH NMR (CDC13) 8 7.56 (d, 1 H), 5.63 (m, 1H), 5.43 (m, 1H), 5.02 (d, 1H), 4.45 (dd, 1H), 3.68 (d, 1H), 3.38 (m, 1H), 2.62-2.4 (m, 3H), 2.07 (s, 3H), 1.96 (m, 1 H), 1.6 (dd, 3H), 1.52 (s, 9H), 1.6-1.48 (m, 2H), 0.88 (t, 3H); MS: (M-H)-= 376; (M+H) + = 378, (M+Na) + = 400 2881 tert-Butvl (2R, 3S. 5R)-2-((1R. 2S)-1-(acetvlamino)-2-hydroxy-2- methylpentyl)-5-cyano-3-((1Z)-1-propenyl)-1-pyrrolidinecarbo xylate A solution of the product of example 288H (20 mg, 0.053 mmol) in THF (0.5 mL) was added to methyl magnesium bromide (3M in THF, 0.105 mL, 0.318 mmol) in THF (1.0 ml) at 0°C. After 0.5 h at 0 °C the mixture was quenched with sat.

NH4CI and extracted with ethyl acetate. The organic layer was separated, dried, and the solvent was evaporated. The crude residue was purified by column chromatography on silica gel using ethyl acetate to give the title compound (10.4 mg, 50%).

'H NMR (CDCI3) 6 5. 91 (d, J=9. 5 Hz, 1H), 5.71 (m, 1 H), 5.55 (m, 1 H), 4.38 (d, J=8.8 Hz, 1 H), 4.28 (m, 1 H), 3.97 (m, 1 H), 3.47 (m, 1 H), 2.55 (m, 1 H), 2.07- 2.02 (m, 1 H), 2.02 (s, 3H), 1.65 (d, J=6.8 Hz, 3H), 1.56 (s, 9H), 1.56-1.30 (m, 4H), 1.14 (s, 3H), 0.93 (m, 3H).

MS: (M+H) + = 394, (M-H)-=392 288Jtert-Butyl (2R,3S,5R)-2-((1R,2S)-1-(acetylamino)-2-methoxy-2- methvlpentvl)-5-cyano-3-(1ZZ)-1-propenyl)-1-pyrrolidinecarbo xKlate To a solution of the product of example 2881 (16 mg, 0.041 mmol) in THF (0.5 mL) at-78 °C were added methyl iodide (0.125 mL, 2.02 mmol) and NaHMDS (1 M in THF, 0.06 mL, 0.061 mmol) and the mixture was stored at-25 °C for 16 h. The reaction was quenched with sat. NH4CI and ethyl acetate was added. The organic layer was separated, dried and the solvent was evaporated.

The crude residue was purified by column chromatography on silica gel using 50% ethyl acetate/dichloromethane to give the title compound (7.7 mg, 50%).

1 H NMR (CDC13) rotamers 5 5.89 (m, 1 H), 5.71 (m, 1H), 5.50 (m, 1 H), 4.55 (dd, J=2.0,9.2 Hz, 1 H), 4.44 (t, J=9.5 Hz, 1 H), 4.36 (dd, J=1.0,9.5 Hz, 1H), 3.94 (m, 1 H), 3.89 (d, J=1.0 Hz, 1 H), 3.73 (dd, J=9. 2, 9. 8 Hz, 1 H), 3.21 and 3.20 (2s, 3H), 2.49 (m, 1H), 2.00 (s, 3H), 1.95 (d, J=12.9 Hz, 1H), 1.80-1.20 (m, 4H), 1.66 (dd, J=1.7,6.8 Hz, 1H), 1.55 (m, 9H), 1.14 and 1.12 (2s, 3H), 0.95 (t, J=6.8 Hz, 3H).

MS: (M+H)+ = 408, (M-H)-=406 288K (-)-(2R,3S, 5R. 1'R. 2'S)-2-(1-Acetamido-2-methoxy-2-methyl)pentyl-3-(cis- propen-1-yl)-pyrrolidine-5-carboxylic Acid Hydrochloride Salt The product of example 288J (2 mg, 0.005 mmol) was combined with hydrochloric acid (6N, 1 mL) and stirred at 60 °C for 16 h. The solvent was evaporated to give the title compound (1.6 mg, quant.).

'H NMR (MeOD-d3) 8 5.60 (m, 1H), 5.30 (m, 1H), 4.48 (d, J=9.8 Hz, 1H), 4.37 (t, J=8.1 Hz, 1H), 3.65 (t, J=9.5 Hz, 1H), 3.27 (s, 3H), 3.27 (m, 1H), 2.57 (m 1 H), 1.93 (s, 3H), 1.72 (m, 1 H), 1.62 (dd, J=1.7,6.8 Hz, 3H), 1.70-1.23 (m, 4H), 1.28 (s, 3H), 0.88 (t, J=6.8 Hz, 3H).

MS: (M+H) + = 327, (M+Na) +=349 Example 289 (-)-(2R. 3S. 5R. 1'R. 2'S)-2-(1-Acetamido-2-methoxv-2-methvl) nentvl-3-(cis-propen-<BR> <BR> 1-vl)-pyrrolidine-5-carboxylic Acid Hydrochloride Salt 289A(2E)-2-Methyl-2-penten-1-ol A solution of methyl 2-methyl-2-pentenoate (6.37 g, 49.6 mmol) in ether (120 mL) was added to a mixture of lithium aluminum hydride (4.72 g, 124.2 mmol) in ether (180 mL) at 0 °C and stirred at 25 °C for 1.5 h. After recooling to 0 °C the reaction was quenched (water, 4.7 mL; 15% NaOH, 4.7 mL; water, 14.1 mL). The mixture was filtered, dried, refiltered, and the solvent was evaporated.

The crude product was distilled (55 torr, 85-87 °C) to give the allylic alcohol (3.96 g, 80%).

'H NMR (CDC13) 8 5.44-5.38 (m, 1H), 4.0 (d, J=5.42Hz, 2H), 2.10-2.00 (m, 2H), 1.67 (s, 3H), 1.0-0.95 (t, J=5.09Hz, 3H).

289B A solution of the product of example 289A (5.1 g, 51 mmol) in dichloromethane (35 mL) was added to a mixture of (-)-dimethyl D-tartrate (0.545 g, 3.06 mmol), titanium tetraisopropoxide (0.76 g, 2.55 mmol), molecular sieves (4A, 1.8 g), and t-butyl hydroperoxide (5M solution in decane, 20 mL) in dichloromethane (180 mL) at-20 °C, and the reaction mixture was stirred for 3.5 h. To this mixture was added trimethyl phosphite (9 mL, 76.5 mmol), triethylamine (8.5 mL, 61.2 mmol), and benzoyl chloride (5.92 mL, 51 mmol), and was stirred for 1.5 h before being washed with 10% tartaric acid (200 mL x2), saturated sodium bicarbonate (150 mL x3), and brine. The organic layer was dried, the solvent was evaporated and the residue was purified by column chromatography on silica gel using hexanes-85% hexanes/ethyl acetate to give the title compound (7.35 g, 65%).

'H NMR (CDC13) 8 8.18-7.42 (m, 5H), 4.31 (dd, J=11. 87,6.45 Hz, 1H), 2.94 (t, J=6.45 Hz, 1 H), 1.71-1.53 (m, 2H), 1.40 (s, 3H), 1.06 (t, J=7.46 Hz, 3H).

289C (2S)-2-Methyl-1, 2-pentanediol To a solution of lithium aluminum hydride (3.8 g, 0.1 mol) at 0 °C in tetrahydrofuran (THF) (160 mL) was added the product of example 289B (7.35 g, 0.033 mol) in THF (40 mL). After 0.5 h, the mixture is warmed to 25 °C, recooled to 0 °C and quenched (water, 5 mL; 15% NaOH, 5 mL; water, 15 mL). The mixture was filtered, and the solvent was evaporated. The residue was purified by column chromatography on silica gel using hexanes-60% hexanes/ethyl acetate to give the title compound (2.48 g, 63%).

'H NMR (CDC13) 8 3.50-3.38 (m, 2H), 1.50-1.31 (m, 4H), 1.17 (s, 3H), 0.94 (t, J=7.1 Hz, 3H).

289D(2S)-1- (Benzyloxy)-2-methvl-2-pentanol To a suspension of sodium hydride (95% powder, 5.08g, 0.212 mol) in THF (210 mL) at 0 °C was added the product of example 289C (10.02 g, 0.085 mol) in THF (85 mL). After 1 h, benzyl bromide (12.1 mL, 0.102 mol) was added, and the reaction mixture was warmed to 25 °C for 16 h. The mixture was quenched with sat. NH4CI (30 mL), and the solvent was evaporated. The residue was partitioned between water and ether, the ether was separated and dried.

The crude product was purified by column chromatography on silica gel using 100-85% hexanes-hexanes/ethyl acetate to give the title compound (17.26 g, 98%).

'H NMR (CDC13) 8 7.39-7.27 (m, 5H), s (4.56,2H), 3.31 (dd, J=8.82,16.96 Hz, 2H), 1.52-1.23 (m, 4H), 1.17 (s, 3H), 0.92 (t, J=7.12,3H).

289E ((((2S)-2-Methoxy-2-methylpentyl)oxy)methyl)benzene To a solution of the product of example 289D (17.26 g, 0.0829 mol) in THF (280 mL) at 0 °C was added sodium bis (trimethylsilyl) amide (NaHMDS) (1M in THF, 166 mL, 0.166 mol). After 1 h methyl iodide (25.8 mL, 0.415 mol) was added, and the mixture was warmed to 25 °C for 16h. The mixture was quenched with sat. NH4CI (25 mL) and water (250 mL), the organic layer was separated and the aqueous layer was extracted with ether. The organic layers were combined, washed with brine, and dried. The solvent was evaporated, and the residue was purified by column chromatography on silica gel using hexanes-90% hexanes/ethyl acetate to give the title compound (18.03 g, 98%).

'H NMR (CDC13) 8 7.35-7.26 (m, 5H), 4.55 (s, 2H), 3.32 (dd, J=9.8,12.5 Hz, 2H), 3.22 (s, 3H), 1.58-1.42 (m, 2H), 1.34-1.22 (m, 2H), 1.14 (s, 3H), 0.90 (t, J=7.12 Hz, 3H).

289F (2S)-2-Methoxy-2-methylpentanal To a solution of the product of example 289E (5 g, 22.5 mmol) in dichloromethane (75 mL) was added Pd (OH) 2 (20% on carbon, 1.6 g) and the flask fitted with a hydrogen ballon. The mixture was stirred for 3.5 h after which the catalyst was filtered and rinsed with dichloromethane (75 mL). This solution was reacted directly with pyridinium chlorochromate (14.5 g, 67.5 mmol) at 0 °C with molecular sieves (4 A, 5 g) and Celite (5g). The mixture was stirred for 1.5 h at 25 °C. Ether (200 mL) was added and this solution was filtered through a short pad of silica gel with additional ether. The solvent was evaporated in a short-path distillation apparatus, and the product was distilled (60 torr, 72-75 °C) to give the title compound (1.4 g, 48% for two steps).

H NMR (CDC13) 8 9.21 (s, 1H), 3.28 (s, 3H), 1.64-1.51 (m, 2H), 1.38-1.23 (m, 2H), 1.22 (s, 3H), 0.92 (t, J=7.12 Hz, 3H).

289G N-((E. 2S)-2-Methoxy-2-methylpentylidene)-(4R)-methylbenzenesulfini mine To a solution of p-toluenesulfinamide (138.42 mg, 0.892 mmol) in THF (3 mL) was added aldehyde product of example 289F (0.143 mL, 1.07 mmol) followed by titanium tetraethoxide (0.57 mL, 2.72 mmol). The solution was stirred at 25 °C overnight and quenched by pouring the solution into brine (3 mL). The mixture was filtered through a pad of Celite and rinsed with dichloromethane and water. The organic layer was separated and the aqueous layer was extracted with dichloromethane (2x 20 mL). The combined organic layers was dried and the solvent was evaporated. The crude residue was purified by column chromatography on silica gel using 50-33% hexane/dichloromethane, then 1 % and 2% Et20/dichloromethane to give the title compound (189.19 mg, 79%).

'H NMR (CDCI3) 6 8.13 (s, 1H), 7.56 (d, J=8.48Hz, 2H), 7.30 (d, J=8.47, 1 H), 3.20 (s, 3H), 2.40 (s, 3H), 1.59 (m, 2H), 1.33 (s, 3H), 1.24 (m, 2H), 0.84 (t, J=7.29Hz, 3H) MS: (M+H) + = 268 289H To a solution of the product of example 289G (0.278 g, 1.04 mmol) and N-t-butoxycarbonyl-2-t-butyidimethylsilyloxypyrrole (0.59 g, 1.98 mmol), prepared according to the procedure described in JOC, 1992, 57,3760-3763, in dichloromethane (7 mL) at-78 °C was added trimethylsilyl triflate (0.283 mL, 1.56 mmol). After 6 h, the reaction was quenched at-78 °C with triethylamine (0.232 mL, 3.15 mmol) followed by saturated sodium bicarbonate (5 mL) and dichloromethane and warmed to 25 °C. The organic layer was separated, dried, and the solvents were evaporated. The crude residue was triturated with ether and the resulted precipitation was filtered to give the title compound as a white solid (0.25 g, 53%).

'H NMR (CDC13) 8 7.56 (d, 2H), 7.28 (d, 2H), 7.29 (dd, 1H), 5.59 (dd, 1H), 4.81 (m, 1 H), 4.27 (dd, 1 H), 4.18 (d, 1H), 3.18 (s, 3H), 2.39 (s, 3H), 1.92 (m, 1 H), 1.38-1.72 (m, 3H), 1.69 (s, 9H), 1.43 (s, 3H), 1.03 (t, 3H) MS: (M+H) + = 451, (M+Na) + = 473 2891 tert-Butvl (3S)-2-((1R. 2S)-2-methoxy-2-methyl-1-(((4- methylphenyl)sulfinyl)amino)pentyl)-5-oxo-3-((1Z)-1-propenyl )-1- pyrrolidinecarboxylate To a suspension of copper bromide/dimethyl sulfide (62.53 mg, 0.30 mmol) in THF (0.6 mL) at-78 °C was added propenylmagnesium bromide (0.5 M in THF, 1.22 mL, 0.61 mmol). The solution was stirred at-78 °C for 30 min. To the solution was added trimethylsilyl chloride (0.017 mL, 0.13 mmol) followed by a solution of the product of example 289H (54.96 mg, 0.12 mmol) in dichloromethane (0.4 mL) at-78 °C. The reaction mixture was stirred at-78 °C for 1.5 h. The reaction mixture was quenched with sat. NH4CI and warmed to 25 °C for 1 h. The mixture was partitioned between saturated NH4CI (10 mL) and dichloromethane (45mL). The organic layer was washed with H20 (10 mL) and the combined aqueous layers was extracted with dichloromethane (2x10 mL).

The combined organic layers were dried, filtered, and the solvent was evaporated. The crude residue was purified by column chromatography on silica gel using 17-33% ethyl acetate/hexane to give the title compound (44.31 mg, 74%).

'H NMR (CDC13) 8 7.62 (d, J=8.47Hz, 2H), 7.32 (d, J=8.48Hz, 2H), 5.43 (m, 2H), 4.58 (d, J=7.80Hz, 1 H), 3.99 (br d, J=2.71 Hz, 1 H), 3.93 (dd, J=7.80, 2.71 Hz, 1 H), 3.71 (m, 1H), 3.19 (s, 3H), 2.57 (dd, J=17. 81,9.67Hz, 1H), 2.40 (s, 3H), 1.97 (dd, J=17. 80, 1.53Hz, 1H), 1.82 (m, 1H), 1.59 (s, 12H), 1.52 (m, 1 H), 1.42 (s, 3H), 1.27 (m, 2H), 0.96 (t, J=6.95Hz, 3H) MS: (M-H)-= 491; (M+H) + = 493, (M+NH4) + = 510, (M+Na) + = 515 289J (1) tert-Butvl (3S. 5R)-5-cvano-2-((1 R. 2S)-2-methoxv-2-methvl-1-(((4- <BR> <BR> <BR> methylphenvl) sulfinyl amino) penyl)-3- ( (1Z-propenyl)pyrrolidinecarboxylate The title compound was prepared according to the procedure described in example 288C substituting the product of example 2891 for the product of example 288B.

'H NMR (CDC13) 8 7.53 (d, J=8.14Hz, 2H), 7.32 (d, J=8.14Hz, 2H), 5.73 (m, 1H), 5.48 (m, 1 H), 4.58-4.46 (m, 2H), 4.01-3.69 (m, 3H), 3.18 (s, 0.6H), 3.16 (s, 2.4H), 2.42 (s, 3H), 2.38 (m, 1H), 1.81-1.66 (m, 3H), 1.61-1.59 (m, 12H), 1.26- 1.17 (m, 5H), 0.92 (m, 3H) MS: (M-H)-= 502; (M+H) + = 504, (M+Na) + = 526 289J (2) Alternative Preparation oftert-Butvt (3S. 5R)-5-cvano-2-1R. 2S)-2- methoxy-2-methyl-1-(((4-methylphenyl)sulfinyl)amino)pentyl)- 3-((1Z)-1-propenyl)- 1-pyrrolidinecarboxylate The title compound was prepared according to the procedure described in example 288C substituting the product of example 2891 for the product of example 288B, with the exception that to a solution of crude alcohol resulting from step (i) (following DiBAl-H reduction) (1.21 g, 2.4 mmol) in dichloromethane (12 mL) at-78 °C was added trimethylsilyl cyanide (0.98 mL, 7.2 mmol) and trimethylsilyl triflate (0.64 mL, 3.6 mmol). After 5 minutes the reaction was quenched with sat. sodium bicarbonate and warmed to 25 °C and extracted with dichloromethane. The solvents were evaporated and the crude residue was purified by column chromatography on silica gel using 40% ethyl acetate/hexane to give the title compound (0.82 g).

289K tert-Butvl (2R, 3S. 5R)-2- !-2-((1 R. 2S)-1-(acetylamino)-2-methoxv-2- methylpentyl)-5-cyano-3- ( ( Z)-1-propenvl)-1-pyrrolidinecarbolate i. To a solution of the product of example 289J (8.4 mg, 0.017 mmol) in MeOH (0.17 mL) was added trifluoroacetic acid (0.0055 mL, 0.071 mmol) at 25 °C. The solution was stirred overnight and the solvents were evaporated. The residue was azeotroped with chloroform and toluene to give an oil, which was used in the next step without purification. ii. To the crude amine from 289K (i) in dichloromethane (0.17 mL) was added triethylamine (0.0238 mL, 0.17 mmol) followed by acetic anhydride (0.0081 mL, 0.086 mmol) at 25 °C. The solution was stirred for 20 min and the solvent was evaporated. The residue was purified by column chromatography on silica gel using 33-67% ethyl acetate/hexane to give the title compound (5.16 mg, 76% over two steps).

289L (-)-(2R. 3S. 5R. 1'R, 2'S)-2-(1-Acetamido-2-methoxy-2-methyl ! pentVl-3-(cis-<BR> <BR> <BR> propen-1-yl)-nyrrolidine-5-carboxelic Acid Hydrochloride Salt The title compound was prepared according to the procedure of example 288K.

Example 290 (-)-(2R,3S,5R,1'R,2'S)-2-(1-Acetamido-2-methoxy-2-methyl)pen tyl-3-(cis- propen-1-yl)-pyrrolidine-5-carboxylic Acid Hydrochloride Salt 290A tert-Butyl (2R)-2-((1R,2S)-2-methoxy-2-methyl-1- ((tritylsulfenyl) amino) pentyl)-5-oxo-2,5-dihydro-1H-pyrrole-1-carboxylate i. A mixture of aldehyde product of example 289F (250 mg, 1.92 mmole), triphenylmethanesulfenamide (560 mg, 1.92 mmole), MgS04 (750 mg, 5.7 mmole) and PPTS (10 mg, 0.04 mmole) was stirred at 25 °C for 18 hours, filtered and the solvent was evaporated. ii. To the crude imine from 290A (i) in ether (6.0 ml) at-78 °C were added N-t-butoxycarbonyl-2-t-butyidimethylsilyoxypyrrole (1.13 g, 3.8 mmole) and BF3- Et20 (0.61 ml, 4.8 mmole) and the reaction was stirred at-78 °C for 2 h and then at-40 °C for 2 h. The reaction was quenched with sat. sodium bicarbonate (30 ml) and allowed to warm to 25 °C. The reaction was diluted with chloroform (75 ml) and the organic layer was washed with water and brine, dried, filtered and the solvents were evaporated. The residue was purified by column chromatography on silica gel using 20% ethyl acetate/hexane to provide the title compound (higher R (821 mg, 73%) and a lower Rf diastereomer (187 mg, 17%).

Majordiasteomer: 'H NMR (CDC13) 6 7.32 (dd, J=2.0,6.1 Hz, 1H), 7.29-7.19 (m, 15H), 6.02 (dd, J=1.4,6.1 Hz, 1H), 4.83 (m, 1H), 3.86 (dd, J=3.1,11.5 Hz, 1H), 3.05 (s, 3H), 2.62 (d, J=11. 2 Hz, 1 H), 1.62-0.98 (m, 4H), 1.36 (s, 9H), 0.92 (t, J=6.6 Hz, 3H), 0.43 (s, 3H).

MS: (M+H) += 587, (M+Na) +=609 Minordiastereomer: 'H NMR (CDC13) 8 7.28-7.18 (m, 16H), 6.02 (dd, J=1.7,6.1 Hz, 1H), 4.90 (m, 1 H), 3.89 (dd, J=2.0,11.5 Hz, 1 H), 3.06 (s, 3H), 2.42 (d, J=11. 5 Hz, 1 H), 1.54 (s, 9H), 1.28-0.85 (m, 4H), 1.16 (s, 3H), 0.57 (t, J=7.1 Hz, 3H).

MS: (M+H)'= 587, (M+Na) +=609 290B tert-Butvl (2R. 3S)-2-((1R. 2S)-2-methoxy-2-methyl-1- ((tritylsulfenyl)amino)pentyl)-5-oxo-3-((1Z)-1-propenyl)-1-p yrrolidinecarboxylate The title compound was prepared according the procedure of example 288B substituting the product of example 290A for the product of example 288A yielding (747 mg, 93%).

'H NMR (CDC13) 8 7.30-7.19 (m, 15H), 5.44-5.33 (m, 2H), 3.99 (d, J=1.4 Hz, 1H), 3.69 (m, 1 H), 3.58 (dd, J=2.4,10.5 Hz, 1H), 3.06 (d, J=10. 9 Hz, 1H), 3.03 (s, 3H), 2.81 (dd, J=9.5,18.0 Hz, 1 H), 1.95 (dd, J=1.4,18.0 Hz, 1 H), 1.58 (d, J=5.1 Hz, 3H), 1.62-1.02 (m, 4H), 1.43 (s, 9H), 0.86 (t, J=6.6 Hz, 3H), 0.51 (s, 3H).

MS: (M+H)+ = 629, (M+Na) +=651 290C tert-Butyl (2R,3S,5R)-5-cyano-2-((1R,2S)-2-methoxy-2-methyl-1- ((tritylsulfenyl amino) pentyl)-3-((1Z)-1-propenyl)-1-pyrrolidinecarboxylate The title compound was prepared according the procedure of example 288C substituting the product of example 290B for the product of example 288B yielding(459 mg, 71%).

'H NMR (CDC13) 8 7.31-7.20 (m, 15H), 5.74-5.67 (m, 1H), 5.49-5.38 (m, 1 H), 4.60 (d, J=9.2 Hz, 1H), 3.82 (dd, J=7.8,10.5 Hz, 1H), 3.79 (d, J=1.7 Hz, 1H), 3.68 (dd, J=2.0,10.5 Hz, 1H), 3.05 (d, J=10. 5 Hz, 1H), 3.01 (s, 3H), 2.59- 2.48 (m, 1H), 1.84 (d, J=13.2 Hz, 1H), 1.60 (dd, J=1.7,6.8 Hz, 3H), 1.62-0.90 (m, 4H), 1.34 (s, 9H), 0.86 (t, J=6.6 Hz, 3H), 0.45 (s, 3H).

MS: (M+H) + = 640, (M+Na) +=662, (M-H)-=638 290D tert-Buyl (2R. 3S. 5R)-2-((1R. 2S)-1-(acetYlamino !-2-methoxy-2-<BR> <BR> <BR> <BR> <BR> <BR> methylnentyl)-5-cvano-3-((1 Z)-1-propenvl)-1-pyrrolidinecarboxvlate The product of example 290C (23.0 mg, 0.036 mmole) was suspended in methanol (1.0 ml) and PPTS (36.0 mg, 0.143 mmol) was added. The mixture was heated to reflux for 15 h and then the solvent was evaporated. To the crude residue dissolved in dichloromethane (0.5 ml) were added acetic anhydride (0.01 ml, 0.108 mmol) and triethylamine (0.03 ml, 0.216 mmol), and the mixture was stirred for 1 h. The reaction was quenched with methanol (0.05 mi) and evaporated. The residue was purified by column chromatography on silica gel using a gradient of dichloromethane up to 50% ethyl acetate/dichloromethane to give the the title compound (11.3 mg, 77%).

290E(-)-(2R. 3S5R, 1'R. 2'S)-2-(1-Acetamido-2-methoxy-2-methyl) pentyl-3-(cis- propen-1-yl)-pyrrolidine-5-carboxylic Acid Hydrochloride Salt The title compound was prepared according to the procedure of example 288K.

Example 291 tert-Butyl (2R)-2-((1R,2S)-2-methoxy-2-methyl-1-(acetylamino)pentyl)-5- oxo-2,5- dihvdro-1H-prrrole-1-carboxyiate 291A Ammonia gas was bubbled through a suspension of phenyl disulfide (34 mg, 0.15 mmoles) and silver nitrate (26 mg, 0.15 mmol) in methanol (2.5 mL) at 0 °C. After 15 min a solution of aldehyde product of example 289F (40 mg, 0.3 mmol) in methanol (0.5 mL) was added and the mixture was stirred at 25 °C for 68 h. The reaction mixture was filtered, evaporated, dissolved in ether, refiltered.

The organic layer was washed. with water, dried, and the solvents were evaporated to give crude imine (42 mg, 57%).

291 B tert-Butvl (2R)-2-((1 R. 2S)-2-methoxy-2-methvl-1- (plienylsulfenylamino) pentyl)-5-oxo-2, 5-dihydro-1 H-pyrrole-1-carboxvlate A solution of imine product of example 291A (42 mg, 0.18 mmol) and N-t- butoxycarbonyl-2-t-butyldimethylsilyloxypyrrole (53 mg, 0.18 mmol) in dichloromethane (1.5 mL) was cooled to-78 °C and boron trifluoride-etherate (0.034 mL, 0.27 mmol) was added. After 2 h at-78 °C the reaction was quenched with sat. sodium bicarbonate (2 mL), extracted with dichloromethane, dried, and the solvent was evaporated to give the title compound.

291C tert-Butyl (2R)-2-((1R,2S)-2-methoxy-2-methyl-1-(acetylamino)pentyl)-5- oxo-2,5-dihydro-1 H-pyrrole-1-carboxylate The product of example 291 B was treated with 80% acetic acid (2 mL) at 25 °C for 1 h and the solvent was evaporated. The crude residue was purified using 5% methanol in dichloromethane with 0.2% ammonium hydroxide to give the amine (28 mg, 50%).

The amine product (28 mg, 0.09 mmol) in dichloromethane (1 mL) was treated with acetic anhydride (0.017 mL, 0.18 mmol), triethylamine (0.028 mL, 0.2 mmol) and 4-dimethylaminopyridine (2 mg, 0.02 mmol) for 1 h. The solution was purified by column chromatography on silica gel directly by eluting with 5% methanol in dichloromethane to give the title compound (26 mg, 82%) as a 1.9-1 mixture of two inseparable diastereomers.

Majorisomer: 'H NMR (CDC13) 8 7.26-7.30 (dd, 1H), 6.05-6.12 (dd, 1H), 5.33 (d, 1H), 4.96-5.01 (dd, 1H), 4.88-4.92 (m, 1 H), 3.25 (s, 3H), 1.88 (s, 3H), 1.61 (s, 9H), 1.4 (s, 3H), 0.9 (t, 3H).

Minorisomer: rH NMR (CDC13) 5 7.31-7.35 (dd, 1 H), 6.05-6.1 (dd, 1H), 5.37 (d, 1H), 4.87-4.92 (dd, 1 H), 4.83-4.88 (m, 1 H), 3.23 (s, 3H), 1.92 (s, 3H), 1.59 (s, 9H), 1.18 (s, 3H), 1.02 (t, 3H).

Example 292 <BR> <BR> tert-Butyl(2R)-2-((1R. 2S)-2-methoxy-2-methvl-1-((4-methoxYanilino) pentyl)-5<BR> <BR> <BR> <BR> oxo-2.5-dihydro-1 H-pyrrole-1-carboxylate To a suspension of Yb (OT3 (12 mg, 019 mmole) and MgSO4 (70 mg, 0.58 mmole) in dichloromethane (0.50 ml) were added the aldehyde product of example 289F (22 mg, 0.19 mmole) in dichloromethane (0.50 ml) and p- anisidine (24 mg, 0.19 mmole) in dichloromethane (0.50 ml) and the mixture was stirred for 0.5 h at rt. N-t-butoxycarbonyl-2-t-butyidimethylsilyloxypyrrole (59 mg, 0.19 mmol) in dichloromethane (0.35 ml) was added and the reaction was stirred for 1 h and then quenched with water. The reaction was diluted with ethyl acetate and the organic layer was washed with water and brine, dried, filtered and the solvents were evaporated. The residue was purified by column chromatography on silica gel using mixtures of ethyl acetate/hexane to provide the title compounds two diastereomers (Yield : higher Rf (25 mg, 31 %), lower Rf (28 mg, 35%)).

Higher Rf diasteomer: rH NMR (CDC13) 5 7.42 (dd, J=2.0,6.1 Hz, 1H), 6.65 (m, 2H), 6.41 (m, 2H), 6.06 (dd, J=1.7,6.1 Hz, 1H), 4.91 (m, 1H), 4.28 (dd, J=3.4,10.5 Hz, 1H), 3.70 (s, 3H), 3.42 (d, J=10. 8 Hz, 1H), 3.26 (s, 3H), 1.90-1.29 (m, 4H), 1.52 (s, 9H), 1.18 (s, 3H), 1.02 (t, J=7.1 Hz, 3H).

MS: (M+H) + = 419, (M+Na) +=441 Lower Rf diastereomer: rH NMR (CDC13) 8 7.39 (dd, J=2.4,6.1 Hz, 1H), 6.65 (m, 2H), 6.40 (m, 2H), 6.05 (dd, J=1.7,6.1 Hz, 1H), 4.93 (m, 1H), 4.29 (dd, J=2.4,10.5 Hz, 1H), 3.70 (s, 3H), 3.45 (d, J=10.5 Hz, 1H), 3.26 (s, 3H), 1.58 (s, 9H), 1.55-1.10 (m, 4H), 1.39 (s, 3H), 0.69 (t, J=7.5 Hz, 3H).

MS: (M+H) + = 419, (M+Na) +=441 Example 293 (-)-(2R, 3S. 5R. 1'R. 2'S)-2-(1-Acetamido-2-methoxy-2-methyl)pentyl-3-(cis- propen-1-yl)-pyrrolidine-5-carboxylic Acid Hydrochloride Salt 293A (R) 4-(Methoxy-methyl-carbamoyl)-2,2-dimethyl-oxazolidine-3-carb oxylic acid tert-butyl ester The title compound was synthesized according to the procedure of A. D.

Campbell, T. M. Raynham, R. J. K. Taylor, Synthesis 1998, 1707 from D-serine, yield 65% (3 steps). [a] D= +36.2° (c=1. 8, CHC13) [ref. [a] D= +35.8°].

'H NMR (400MHz, Ceci3) : 84. 78 (dd, 0.5H, 1/2 NCH, J=3.5 and 7.4Hz); 4.68 (dd, 0.5H, 1/2 NCH, J=3.5 and 7.4Hz); 4.15 (m, 1H,) ; 3.95 (m, 1H, OCH); 3.72 (s, 1.5 H, 1/2 OMe); 3.68 (s, 1.5H, 1/20Me) ; 3.18 (s, 3H, Nme); 1.72 (s, 1.5H, 1/2Me) ; 1.68 (s, 1.5H, 1/2 Me); 1.58 (s, 1.5H, 1/2 Me) ; 1.54 (s, 1.5 H, 1/2 Me); 1.48 (s, 4.5 H, 1/2 CMe3); 1.40 (s, 4.5 H, 1/2 CMe3).

293B (R) 4-But-3-enoyl-2, 2-dimethyl-oxazolidine-3-carboxylic acid ter-but ester A solution of the product of example 293A (2.0 g, 6.96 mmol) in dry THF (20 mL) was added a 1.0 M solution of allyl magnesium chloride (14 mL, 14 mmol) at-78°C under N2. The resulting yellow solution was stirred at-65 °C. TLC indicated the absence of S. M. after 1 h. The mixture was quenched with saturated. NH4CI at-65°C and warmed up to r. t.. After extraction with EtOAc, the crude product was purified by flash chromatography (EtOAc/hexane 2: 8) to give the title compound as a colorless oil (1.74 g, yield 93%).

[a] D= +67.8° (c=2.5, CHC13).

1H NMR (400MHz, CDC13) : 8 5.90 (m, 1H, =CH) ; 5.19 (m, 2H, =CH); 4.49 (m, 0.5H, 1/2 NCH); 4.36 (m, 0.5H, 1/2 NCH)'4.12 (m, 1 H, OCH); 3.90 (m, 1H, OCH); 3.25 (m, 2H, COCH2) ; 1.71 (s, 1.5 H, 1/2 Me); 1.65 (s, 1.5 H, 1/2 Me); 1.52 (s, 1. 5H, 1/2 Me); 1.49 (s, 6H, 1/2 Me, 1/2 CMe3) ; 1.41 (s, 4.5 H, 1/2 CMe3) . MS (FAB): 270.1 (M+1), 214.1,200.1,170.1,156.0,137.0,100.1,83.0, 69.0 HRMS (FAB, NBA): calcd. for C14H24NO4 (MH) 270.1705, found 270.1713 293C (4R, 1'S) 4-(1-Hydroxy-1-methyl-but-3-enyl)-2,2-dimethyl-oxazolidine 3- carboxylic acid tert-butyl ester To a solution of the product of example 293B (2.69 g, 10 mmol) in 50 mL dry THF was added a 1.4 M solution of MeMgCI (21.43 mL, 30 mmol) at-78°C.

The resulting solution was stirred and allowed to warm up to 0°C over 3 h. After quenching with sat. NH4CI, the crude product was purified by flash chromatography (EtOAc: CH2CI2 1: 9) to give 1.29 g of starting material and the title compound (1.37 g, yield 48%) as a colorless oil.

1H NMR (400MHz, CDC13) : 8 5.94 (m, 1H, =CH); 5.05 (dd, 2H, =CH, J=6.8 and 16.3Hz); 3.95 (m, 2H, OCH, NCH); 3.85 (d, 1H, OCH, J=7.2Hz); 2.27 (dd, 1H, CH-C=C, J=5.8 and 13.8Hz); 2.05 (m, 1H, CH-C=C); 1.54 (s, 3H, CMe); 1.46 (s, 3 H, CMe); 1.45 (s, 9H, CMe3) ; 1.05 (s, 3H, Me).

13C NMR (100MHz, CDC13) : 8 155. 38,134.31,117.46,94.49,81.26,73.99, 66.19,64.63,41.84,28.11,26.05,24.48,24.19.

MS (FAB): 286.2 (M+1), 230.1,212.1,200.1,186.1,172.1,154. 1,144.1, 130.0,110.1,100.1,83.0,69.0.

HRMS (FAB, NBA) : calcd. for C15H28NO4 (MH) 286.2018, found 286.2013 293D (4R, 1'S) 4-(1-Methoxy-1-methyl-but-3-enyl)-2,2-dimethyl oxazolidine 3- carboxylic acid tert-butyl ester To a stirred solution of the product of example 293C (0.5 g, 1.75 mmol) in dry THF (5 mL) was added 218 pi Mel (3.51 mmol) at 0°C, then 60% NaH (77 mg, 1.93 mmol) was added followed by Bu4NBr (112 mg, 0.35 mmol). The mixture was stirred at r. t. overnight and quenched with H20 at 0°C. After extraction with EtOAc, the crude oil was purified by flash chromatography (EtOAc: hexane 1: 9) to give the title compound as a colorless oil (367 mg, yield 70%).

[α]D= -26.8° (c=4.4, CHOs) 1H NMR (400MHz, Ceci3) : 8 5.83 (m, 1 H, =CH); 5.03 (m, 2H, =CH2); 4. 14 (d, 2H, OCH2, J= 9.2 Hz); 3.82 (dd, 1H, NH, J-6.5 and 9.2Hz); 3.17 (s, 3H, OCH3) ; 2.53 (dd, 1H, CH-C=C, J=5.9 and 14.9 Hz); 2.10 (m, 1H, CH-C=C); 1.56 (s, 3H, CMe); 1.45 (s, 12H, CMe, CMe3); 1.10 (s, 3H, Me).

13C NMR (100MHz, CDCI3) : 6154. 28, 134.09,117.23,80.08,78.46, 63.77,60.82,49.35,39.95,28.14,26.59,24.32,19.25.

MS (FAB) : 300. 3 (M+1), 260.2,244.2,212.2,200.2,186.1,172.1,154.1, 144.1,99.1.

293E (1R, 2S) (1-Hydroxymethyl-2-methoxy-2-methyl-pent-4-enyl)-carbamic acid tert-butyl ester To a stirred solution of the product of example 293D (1.09 g, 3.65 mmol) in dry MeOH (30 mL) was added p-toluensulfonic acid (70.4 mg, 0.37 mol) and the resulting mixture was stirred at r. t for 30 min. The reaction was quenched with sat. NaHCO3. The solvent was removed under reduced pressure and 20 mL H20 was added. After extraction with EtOAc, the organic layer was dried over MgS04 and evaporated to give the title compound as a white crystalline solid (850 mg, yield 90%).

[a] D=-35. 3° (c=0.9, CHC13).

1 H NMR (400MHz, CDC13) : 8 5.75 (m, 1H, =CH); 5.26 (d, 1 H, NH, J=8.8 Hz); 5.10 (m, 2H, =CH2), 3.94 (dd, 1 H, OCH, J=3.1 and 10.9 Hz); 3.60 (m, 2H, OCH, NCH); 3.21 (s, 3H, OMe); 2.54 (dd, 1H, CH-C=C, J=6.6 and 14.0 Hz); 2.29 (dd, 1 H, CH-C=C, J= 8.0 and 14.0 Hz); 1.43 (s, 9H, CMe3) ; 1.24 (s, 3H, CMe).

13C NMR (CDCI3) : 8 156.24,132.64,118.82,80.68,. 79.33,62.85,55.78, 49.10,40.09,29.56,28.23,19.27.

MS (FAB) : 260.2 (M+1), 242.1,229.1,204.1,172.1,154.0,145.1,136.0, 119.1,109.1,95.1,81.1,69.0.

HRMS (FAB, NBA): calcd. for C13H26NO4 (MH) 260.1862, found 260.1867 293F (1R. 2S) (1-Hydroxymethyl-2-methoxy-2-methyl-pentyl)-carbamic acid tert- butyl ester To a solution of the product of example 292E (259 mg, 1.0 mmol) in MeOH (10 mL) was added 15 mmol% of 10% Pd/C. The flask was then flashed with nitrogen and finally with hydrogen. The reaction mixture was hydrogenated under atmospheric pressure at r. t. for 14 h, after which it was filtered through a pad of Celite (3x10 mL EtOH rinse) and concentrated to give the title compound as a white solid (258 mg, yield 99%).

[a] D=-34. 1° (c=2.2, CHC13).

1H NMR (CDC13) : 8 5.20 (d, 1H, NH, J= 8.7 Hz); 3.68 (dd, 1 H, OCH, J=3.9 and 11. 1Hz) ; 3.55 (m, 2H, NCH, OCH); 3.13 (br, 1 H, OH); 3.07 (s, 3H, OMe); 1.55 (m, 1 H, CH); 1.32 (s, 9H, CMe3) ; 1.25 (m, 3H, CH2, CH); 1.06 (s, 3H, Me); 0.81 (t, 3H, CH3, J= 7.2 Hz).

13C NMR (CDC13) : 8 156.28,80.13,78.98,62.49,55.77,48.60,37.36,28.10,19.11,17.01 ,14.45.

MS (FAB): 262.2 (M+1), 230.2,206.1,174.1,156.1,144.1,123.1,113.1, 101.1,83.1,69.1.

HRMS (FAB, NBA): calcd. for C13H28NO4 (MH) 262.2018, found 262.2024 293G (1S. 2S) (1-Formyl-2-methoxv-2-methyl-pentyl)-carbamic acid tert-butyl ester To a solution of the product of example 293F (190 mg, 0.73 mmol) in dry CH2CI2 (12 mL) was added dry DMSO (2 mL) followed by Et3N (1.01 mL, 7.3 mmol) and Py. S03 (928 mg, 5.84 mmol) at 0°C. The resulting mixture was allowed to warm up to r. t. and stirred for 2 h. The reaction was quenched with sat. NH4CI. After extraction with CH2CI2, the organic layer was dried over MgS04.

The solvent was evaporated to give the title compound as a crude oil (181 mg, yield 96%) which was used without further purification.

1H NMR (400MHz, CDCI3) : 8 9.63 (s, 1H, CHO); 5.18 (d, 1 H, NH, J=7.5Hz); 4.23 (d, 1H, NCH, d=8. 0Hz) ; 3.09 (s, 3H, OMe); 1.50 (m, 2H, CH2); 1.32 (s, 9H, CMe3); 1.20 (m, 2H, CH2) ; 1.13 (s, 3H, CMe) ; 0.82 (t, 3H, CH3, J= 7.1 Hz).

'3C NMR (400MHz, Clos) : 8 201.03,155.67,79.69,79.69, 79.40,63.58, 49.15,37.12,28.00,19.41,16.37,14.24.

293H To a solution of the product of example 292G (272 mg, 1.05 mmol) in dichloromethane (20 mL) was added N-p-methoxybenzyl-N-hydroxylamine (153 mg, 1.0 mmol) followed by a large excess of MgS04. The mixture was stirred at r. t. for 4 h. After filtration and evaporation, the crude product was purified by flash chromatography (EtOAc: hexane 1: 1) to give the nitrone title compound (335 mg, 85%) as a colorless oil.

[a] D= 32.3° (c=2.0, CHC13).

1 H NMR (400MHz, Ceci3) : 8 7.28 (d, 2H, ArH, J=8.5 Hz); 6.85 (d, 2H, ArH, J=8.5 Hz); 6.64 (d, 1 H, NH, J=6.5Hz); 6.05 (br, 1H, CH=N); 4.83 (m, 1 H, CHN); 4.77 (s, 2H, CH2Ar); 3.76 (s, 3H, OMe); 3.09 (s, 3H, OMe); 1.37 (s, 9H, Boc); 1.50-1.20 (m, 4H, 2xCH2); 1.11 (s, 3H, Me); 0.78 (t, 3H, CH3, J=6.2Hz)..

'3C NMR (400MHz, Ceci3) : 8 159. 94,155.45,136.07,130.63, 124.98,114.10, 80.79,79.34,69.51,55.19,53.03,49.40,37.07,28.19,19.43,16.69, 14.56.

2931 To a solution of t-butyl propiolate (505 mg, 4.0 mmol) in dry THF (15 mL) at-78°C was added n-BuLi (1.6 mL, 2.5 M in hexanes, 4.0 mmol) and the resulting solution was stirred for 1 hour. A cold (-78°C) solution of nitrone product of example 293H (395 mg, 1.0 mmol) in dry THF (10 mL) was then added with a canula, followed by BF3. Et2O (568mg, 4.0 mmol). The reaction mixture was stirred at-78°C for an additional 1 h. After being quenched with sat. NH4CI. (5 mL), the mixture was warmed up to r. t. and extracted with EtOAc (3 x 25 mL).

The organic extracts were combined, washed with brine, dried over MgS04 and filtered. The solvent was removed under reduced pressure and the residue was purified by flash chromatography (EtOAc: hexane 2: 8) to give the title compound (417 mg, 80%) as a pale yellow oil.

[a] D=-106. 5° (c=1. 0, CHCI3).

1 H NMR (400MHz, CDC13) : 5 7.29 (d, 2H, ArH, J=8.6 Hz); 6.84 (d, 2H, ArH, J=8.6 Hz); 6.15 (br, 1H, NOH); 5.16 (d, 1H, NH, J=10. 8Hz); 4.25 (m, 1H, CHN); 4.12 (s, 2H, CH2Ar); 3.97 (s, 1 H, CHN); 3.79 (s, 3H, OMe); 3.11 (s, 3H, OMe); 1.51 (s, 9H, t-Bu) ; 1.45 (s, 9H, Boc); 1.35-1.20 (m, 4H, 2xCH2); 1.13 (s, 3H, Me); 0.86 (t, 3H, CH3, J=7.2Hz)..

13C NMR (400MHz, Ceci3) : 8 158. 70,156.99,152.35,130.29,130.03,129.38, 113.44,83.28,81.49,80.04,77.93,60.31,55.10,48.90,36.56,28.23 ,27.89, 19.58,16.34,14.42. 293J (2R. 1'R, 2'S)-1-p-Methoxybenzyl-2- (1-t-butoxycarbonylamino-2-methoxy-2- methyl)pentyl-5-oxo-2. 5-dihydro-1 H-prrole To a solution of the product of example 2931 (239 mg, 0.5 mmol) in a mixture of acetic acid and methanol (1: 9,10 mL) was added zinc dust (20 eq., 10 mmol). The reaction mixture was vigorously stirred for 3 h, during which fresh zinc dust (10 eq.) was added. After filtration through a pad of celite, the residue was washed with dichloromethane (20 mL) and the filtrate was neutralized by NaHCO3 at 0°C. The resulting crude product (after extraction into ethyl acetate, washing with brine, drying over MgS04 and evaporation of the solvent) was purified by flash chromatography (EtOAc: hexanes, 4: 6) to give the title compound (112 mg, 50%) as colorless crystals. The structure and absolute configuration were confirmed by single crystal X-ray crystallography.

1H NMR (400MHz, Ceci3) : 8 7.16 (d, 2H, ArH, J=8.0 Hz); 6.82 (d, 2H, ArH, J=8.0 Hz); 6.16 (d, 1H, =CH, J=6.0Hz); 5.27 (m, 1H, =CH); 4.51 (d, 1H, NH, J=10. 2Hz); 4.10 (m, 1H, CHN); 4.09 (s, 2H, CH2Ar); 3.78-3.75 (m, 1H, CHN); 3.76 (s, 3H, OMe); 3.13 (s, 3H, OMe); 1.50-1.40 (m, 2H, CH2) ; 1.40 (s, 9H, Boc) ; 1.13 (s, 3H, Me); 1.09-0.84 (m, 2H, CH2); 0.62 (t, 3H, CH3).

'3C NMR (400MHz, Ceci3) : 8 171.28,158.83,155.94,145.36,129.41,129.33, 128.01,113.89,113.58,79.57,78.26,60.92,55.11,54.09,48.70,42. 44,37.20, 36.69,28.16,27.99,19.71,17.13,14.38.

293K (2R. 3S. 1'R, 2'S)-1-p-Methoxybenzyl-2- (1-t-butoxycarbonylamino-2- methoxv-2-rnethy!)pentv!-5-oxo-Dvrrotidine The product of example 293J is reacted with the appropriate bis (Z-propen- 1-yl) cuprate according to the procedure of Hanessian in Synthetic Letters 1990, p501-505 to provide the title compound.

293L 2R. 3S. 1'R, 2'S)-1-p-Methoxybenzyl-2- (1-acetamido-2-methoxy-2- methvl)pentyl-5-oxo-pyrrolidine The title compound is prepared according to the procedure of 289K substituting the product of example 293K for the product of example 289J.

293M (2R, 3S, 1'R. 2'S)-1-t-Butoxycarbonyl-2-(1-acetamido-2-methoxy-2- methvt)pentvf-5-oxo-ovrro!idine The product of example 293L is reacted with ceric ammonium nitrate according to the procedure of Yamaura in Bull. Chem. Soc. Japan 1985,58,1413.

The resulting product is reacted with di-t-butyldicarbonate, and N, N- dimethylaminopyridine and triethylamine to give the title compound.

293N (2R, 3S, 5R, 1'R. 2'S)-1-t-Butoxycarbonyl-2- (1-acetamido-2-methoxy-2- methyl)pentyl-5-cyano-pyrrolidine The title compound is prepared according to the procedure described in example 288C substituting the product of example 293M for the product of example 288B. 2930 (-)- (2R, 3S. 5R, 1'R. 2'S)-2-y1-Acetamido-2-methoxy-2-methvl) penyl-3- (cis- propen-1-yl)-pvrrolidine-5-carboxvlic Acid Hvdrochloride Salt The title compound is prepared according to the procedure of example 288K.

Example 294 (~)-(2R,3S,5R,1'R,2'S)-2-(1-Acetamido-2-methoxy-2-methyl)pen tyl-3- [(aminoimino)methyl]amino-pyrrolidine-5-carboxylic Acid. 294A (i)-(2R, 3R, 5R, 1'R, 2'S)-1-t-Butoxycarbonvl-2-(1-acetamido-2-methoxy-2- methyl) pentyl-3-1-hydroxy-2-hydroxy-propane)-pyrrolidine-5-carboxyl ic Acid t- ButylEster.

The product of example 265A is reacted with with osmium tetroxide (0.05 eq.) and 4-N-methylmorpholine-N-oxide (3 eq.) in acetone and water at room temperature. When complete, the reaction is quenched aqueous sodium thiosulfate and partitioned between saturated NH4CI and ethyl acetate. The organic layer is washed with water, and brine, dried over MgS04, filtered and concentrated in vacuo. The residue is then purified by chromatography on silica gel providing the title compound.

294B(tL (2R. 3R. 5R, 1'R, 2'S)-1-t-Butoxycarbonyl-2- (1-Acetamido-2-methoxy-2-<BR> methyl) pentyl-3-formylpvrrolidine-5-carboxylic Acid t-Butyl Ester.

The product of example 294A with sodium periodate (1.5 eq.) in tetrahydrofuran and water at room temperature. When complete, the reaction is quenched with saturated NH4CI and diluted with ethyl acetate. The organic layer is washed with water, and brine, dried over MgSO4, filtered and concentrated in vacuo. The residue is purified by chromatography on silica gel providing the title compound.

294C (~)-(2R,3S,5R,1'R,2'S)-1-t-Butoxycarbonyl-2-(1-acetamido-2-m ethoxy-2- methyl) pent-3-carboxvpyrrolidine-5-carboxylic Acid t-Butvl Ester.

The product of example 294B is reacted with with sodium chlorite (14 eq.) and sodium hydrogen phosphate (10 eq.) in 2-methyl-2-butene, tert-butanol, acetonitrile and water at 0 °C. When complete, the reaction is quenched with aqueous sodium thiosulfate and diluted with ethyl acetate. The organic layer is washed with water, and brine, dried over MgS04, filtered and concentrated in vacuo to provide the title compound. 294D (~)-(2R,3S,5R,1'R,2'S)-1-t-Butoxycarbonyl-2-(1-acetamido-2-m ethoxy-2- methyl)pentyl-3-benzyloxycarbonylamino-pyrrolidine-5-carboxy lic Acid t-Butyl Ester.

The product of example 294C is reacted with diphenylphosphoryl azide (1.2 eq.), triethylamine (1.2 eq.), and benzyl alcohol (1.3 eq.) in toluene at 75 °C. When complete, the reaction is quenched with saturated NH4CI and diluted with ethyl acetate. The organic layer is washed with water, and brine, dried over MgS04, filtered and concentrated in vacuo. The residue is then purified by chromatography on silica gel providing the title compound.

294E (t)- (2R. 3R. 5R, 1'R. 2'S)-1-t-Butoxycarbonyl-2- (1-acetamido-2-methoxy-2- methv!)pentv!-3-amino-pyrrotidine-5-carboxy)ic Acid t-Buyl Ester.

The product of example 294F is reacted with 10% palladium on carbon under an atmosphere of hydrogen in ethanol. When complete, the reaction is diluted with ethyl acetate. The organic layer is washed with water, and brine, dried over MgS04, filtered and concentrated in vacuo. The residue is then purified by chromatography on silica gel providing the title compound.

294F (i)-(2R, 3R, 5R, 1'R, 2'S)-1-t-Butoxycarbonvl 2-(1-acetamido-2-methoxv-2- methyl) pentyl-3-[ (N-tert-butoxycarbonylamino-N'-tert- butoxyCarbonviimino) methvilamino-pvrrolidine-5-carboxylic Acid t-Butvl Ester.

The product of example E is reacted with 1,3-bis (tert-butoxycarbonyl)-2- methyl-2-thiopseudourea (1.5 eq.), triethylamine (5.0 eq.), and mercury (II) chloride (1.5 eq.) in dimethyl formamide at room temperature. When complete, the reaction is diluted with ethyl acetate and filtered through a pad of celite. The organic solution is then washed with water, and brine, dried over MgS04, filtered and concentrated in vacuo. The residue is then purified by chromatography on silica gel providing the title compound. 294G (t)- (2R, 3R. 5R, 1'R. 2'S)-1-t-Butoxvcarbony1-acetamido-2-methoxy-2- methyl)pentyl-3-[(aminoimino)methyl]amino-pyrrolidine-5-carb oxylic Acid.

The product of example 294F is reacted with 80% trifluoroacetic acid in dichloromethane at room temperature. When complete, the reaction is concentrated in vacuo and purified by cation-exchange chromatography on Dowex 50W-X8 providing the title compound.

Example 295 (2R, 3R, 5R, 1'R, 2'S)-2- (1-Acetamido-2-methoxy-2-methyl) pentyl-3- [(aminoimino)methyl]amino-pyrrolidine-5-carboxylic Acid.

295A (2R, 3R, 5R, 1'R, 2'S)-1-t-Butoxycarbonvl-2- (1-acetamido-2-methoxv-2- <BR> <BR> <BR> methVl) pentvl-3-benzyloxvcarbonvlamino-pyrrolidine-5-carboxylic Acid t-Butyl Ester.

The title compound is prepared by chiral HPLC chromatography by the procedure of example 267 substituting the product of example 294D for the product of example 266 to provide the title compound.

295B (2R, 3R, 5R, 1'R, 2'S)-1-t-Butoxycarbonyl-2-(1-acetamido-2-methoxy-2- methyl) pentyl-3-amino-pyrrolidine-5-carboxylic Acid t-Butyl Ester.

The title compound is prepared according to the procedure of example 294E substituting the product of example 295A for the product of example 294D.

295C (2R, 3R, 5R, 1'R, 2'S)-1-t-Butoxycarbonyl-2-(1-acetamido-2-methoxy-2- <BR> <BR> methyl) penyl-3- [ (N-t-butoxycarbonylamino-N'-t-<BR> <BR> butoxycarbonylimino)methyl]amino-pyrrolidine-5-carboxylic Acid t-Butyl Ester.

The title compound is prepared according to the procedure of example 294F substituting the product of example 295B for the product of example 294E.

295D (2R, 3R. 5R. 1'R. 2'S)-1-t-Butoxycarbonyl 2-(1-acetamido-2-methoxy-2- methyl)pentyl-3-[(N-t-butoxycarbonylamino-N'-t- butoxycarbonylimino) methyllamino-pyrrolidine-5-carboxylic Acid t-Butyl Ester.

The title-compound is-prepared-according-to the-procedure of example 294G substituting the product of example 295C for the product of example 294F.

Example 296 (2R. 3R. 5R, 1'R, 2'S)-2-(1-Acetamido-2-methoxy-2-methvl) pentyl-3-amino- pyrrolidine-5-carboxylic Acid.

The title compound is prepared according to the procedure of example 294G substituting the product of example 295B for the product of example 294F.

Example 297 (2R. 3S, 5R, 1'R. 2'S)-2-? (1-Acetamido-2-methoxy-2-methyl) pentyl-3- (cis-propen-1- yl) pvrrolidine-5-carboxylic Acid 3-Pentyl Ester: A mixture of 2.92 g (24.5 mmol, 1.79 mL) thionyl chloride and 20 mL of 3- pentanol was stirred at room temperature for 45 minutes. To this mixture was adden in one portion the product of example 273 (0.40 g, 1.23 mmol). The mixture was stirred at room temperature for 18 h and then the solvent was evaporated in vacuo. The residue was purified by column chromatography on silica gel using 98: 2: 0.1 dichloromethane/methanol/ammonium hydroxide to provide the title compound (yield : 0.325 g, 67%) as a colorless oil.

1H NMR (CDC13) 6 5.5-5.25 (m, 3H), 4.77 (m, 1H), 4.16 (dd, J=10.2,7.2 Hz, 1H), 3.84 (t, J=7.5 Hz, 1H), 3.29 (t, J=7.4 Hz, 1H), 3.18 (s, 3H), 2.96 (quintet, J=8.3 Hz, 1H), 2.34 (td, J=12.5,8.2 Hz, 1H), 1.94 (s, 3H), 1.62 (dd, J=6.5,1.3 Hz, 3H), 1.65-1.25 (m, 8H), 1.11 (s, 3H), 0.95-0.80 (m, 9H).

MS: (M+H) + = 397, (M-H)-= 419, (M-H)'= 395 Example 298 (2R, 3S. 5R, 1'R. 2'S)-2-f1-Acetamido-2-methoxv-2-methv))Dentv)-3-c/s- propen-1-yl)-5-(N-propylcarbamoyl-pyrrolidine The product of example 267 (0.375 g, 1.06 mmol) was reacted with n- propylamine (5 mL) for 5 days room temperature. The reaction was concentrated evaporated in vacuo. The residue was purified by column chromatography on silica gel using 98: 2: 0.1 dichloromethane/methanol/ ammonium hydroxide to provide the title compound (yield : 0.216 g, 56%) as a white solid. aH NMR (CDCl3) 8 7.57 (m, 1H), 5.82 (d, J=9.5 Hz, 1), 5.45-5.35 (m, 1H), 5.24 (m, 1 H), 4.09 (dd, J=9.5,4.8 Hz, 1H), 3.71 (t, J=8.2 Hz, 1H), 3.17 (m, 2H), 3.14 (s, 3H), 2.92 (quintet, J=8.4 Hz, 1H), 2.36 (td, J=13. 2,7.8 Hz, 1H), 2.01 (s, 3H), 1.61 (dd, J=6.8,1.7 Hz, 3H), 1.6-1.25 (m, 6H), 1.12 (s, 3H), 0.94 (t, J=7.1 Hz, 3H), 0.92 (t, J=7.1 Hz, 3H).

MS: (M+H)+ = 367.

Example 299 (i)-(2R. 3S. 5R. 1'R, 2'S}-2-(1-Acetamido-2-methoxv-2-methyl) nentyl-3-(cis-<BR> <BR> <BR> <BR> <BR> propen-1-yl)-5-N-methylcarbamovl-pyrrolidine A mixture of the product of example 267 (0.0085 g, 0.024 mmol) and 3 mL of 2.0 M methylamine in water was stirred at room temperature for 24 h, and the solvent was then evaporated in vacuo. The residue was purified by column chromatography on silica gel using 98: 2: 0.1 dichloromethane/methanol/ ammonium hydroxide to provide the desired product (yield : 0.004 g, 50%) as a colorless oil.

'H NMR (CDC13) 8 7. 54 (m, 1 H), 5.81 (d, J=9.8 Hz, 1), 5.45-5.35 (m, 1H), 5.23 (m, 1H), 4.10 (dd, J=9.5,4.7 Hz, 1H), 3.73 (t, J=8.1 Hz, 1H), 3.17 (dd, J=7.5,4.7 Hz, 1H), 3.14 (s, 3H), 2.93 (quintet, J=8.5 Hz, 1H), 2.79 (d, J=5.1 Hz, 3H), 2.36 (td, J=13. 2,7.8 Hz, 1H), 2.01 (s, 3H), 1.60 (dd, J=6.8,1.7 Hz, 3H), 1.6- 1.25 (m, 4H), 1.12 (s, 3H), 0.94 (t, J=7.1 Hz, 3H).

MS: (M+H)+ = 339 Example 300 (~)-(2R,3S,5R,1'R,2'S)-2-(1-acetamido-2-methoxy-2-methyl)pen tyl-3- (cis-propen-1-vl) pyrrolidine-5-carboxamide : A mixture of 0.0446 g (0.126 mmol)) ()- (2R, 3S, 5R, 1'R, 2'S)-2- (1-acetamido-2- methoxy-2-methyl) pentyl-3- (cis-propen-1-yl) pyrrolidine-5-carboxylic acid ethyl ester and 3 mL of conc. ammonium hydroxide was stirred at room temperature for 24 h, and the solvent was then evaporated in vacuo. The residue was purified by column chromatography on silica gel using 95: 5: 0.1 dichloromethane/methanol/ammonium hydroxide to provide the title compound (yield : 0.0185 g, 45%) as a colorless oil.

'H NMR (MeOH-d4) 8 5.45-5.25 (m, 2H), 4.09 (d, J=8.8 Hz, 1H), 3.75 (dd, J=8.5,7.8 Hz, 1 H), 3.21 (s, 3H), 3.17 (dd, J=8.7,7.0 Hz, 1 H), 2.96 (quintet, J=7.8 Hz, 1H), 2.31 (td, J=12.5,7.6 Hz, 1H), 1.90 (s, 3H), 1.59 (dd, J=6.4,1.4 Hz, 3H), 1.55-1.25 (m, 5H), 1.23 (s, 3H), 0.87 (t, J=7.1 Hz, 3H).

MS: (M+H) + = 326, (M+Na) + = 348, (M-H)-= 324.

Example 301 <BR> <BR> (2R.3S.5R.1'R.2'S)-N-ethv!-2-(1-acetamido-2-methoxv-2-methv) )Dentv)-3-(c/s-<BR> <BR> <BR> <BR> <BR> <BR> propen-1-yl)-5-N-ethylcarbamoyl-pyrrolidine A mixture of 0.0853 g (0.162 mmol) (-)- (2R, 3S, 5R, 1'R, 2'S)-2- (1-acetamido-2- methoxy-2-methyl) pentyl-3- (cis-propen-1-yl) pyrrolidine-5-carboxylic acid ethyl ester toslyate salt and 5 mL of 70% aqueous ethylamine was stirred at rt for 24 h, and the solvent was then evaporated in vacuo. The residue was purified by column chromatography on silica gel using 97.5: 2.5: 0.1 dichloromethane/methanol/ammonium hydroxide to provide the desired product (yield: 0.051 g, 89%) as a white solid.

1H NMR (CDC13) 8 7.51 (m, 1 H), 5.83 (d, J=9.5 Hz, 1H), 5.45-5. 35 (m, 1H), 5.24 (m, 1 H), 4.09 (dd, J=9.5,4.8 Hz, 1 H), 3.70 (t, J=7. 9 Hz, 1 H), 3.26 (dq, J=5.8,7.5Hz, 2H), 3.16 (m, 1H), 3.14 (s, 3H), 2.92 (quintet, J=8.4 Hz, 1H), 2.35 (td, J=12.9,7.8 Hz, 1H), 2.01 (s, 3H), 1.61 (dd, J=6.7,1.5 Hz, 3H), 1.6-1.25 (m, 5H), 1.13 (t, J=7.3 Hz, 3H), 1.12 (s, 3H), 0.94 (t, J=7.1 Hz, 3H).

MS : (M+H) + = 354, (M+Na) + =376, (M-H)-= 352.

Example302 (2R. 3S. 5R. 1'R, 2'S)-2-(1-acetamido-2-methoxy-2-methyl)pentyl-3-(cis-propen- 1- yI- (1-propyl carbamol-pyrrolidine p-Toluenesulfonic Acid Salt : A mixture of (2R, 3S, 5R, 1'R. 2'S)-2-(1-acetamido-2-methoxv-2-methVl) pentyl-3- (cis-propen-1-v)-5-1-propyl) carbamoyl-pyrrolidine (0.213 g, 0.58 mmol) and p-toluenesulfonic acid monohydrate (0.110 g, 0.58 mmol) in 5 mL of dichloromethane was stirred at rt for 15 min. The solvent was evaporated in vacuo to provide-the-desired-product- (yield :-0. 32 g, 99%) as a white powder.

'H NMR (CDC13) 8 9.35 (bs, 1H), 8.3 (bs, 1H), 8.21 (t, J=5.4 Hz, 1H), 7.79 (d, J=8.2 Hz, 2H), 7.16 (d, J=8.1 Hz, 2H), 6.49 (d, J=9.8 Hz, 1H), 5.51 (dq, J=10. 9, 7.8Hz, 1H), 5.27 (m, 1H), 4.76 (m, 1H), 4.56 (dd, J=9.8,7.1 Hz, 1H), 3.81 (m, 1H), 3.42 (m, 1H), 3.25-3.0 (m, 2H), 3.15 (s, 3H), 2.72 (dt, J=13.2,7.5 Hz, 1H), 2.34 (s, 3H), 2.00 (s, 3H), 1.86 (m, 1 H), 1.64 (s, 3H), 1.57 (dd, J=6.9,1.5 Hz, 3H), 1.5-1.2 (m, 2H), 1.19 (s, 3H), 0.84 (t, J = 7.1 Hz, 3H), 0.82 (t, J=6.8 Hz, 3H).

MS: (M+H) + = 367.

Using the methods described above and the general knowledge of one skilled in the art, compounds of the invention (having the indicated relative stereochemistry or enantiomerically enriched and having the indicated absolute stereochemisty) can be prepared which are represented by taking one core from Table 1 (wherein Ac is acetyl), one Y substituent from Table 2, one R substituent from Table 3 and one R3 substituent from Table 4a, 4b, 4c, 4d, 4e, 4f, 4g or 4h.

Table 1 Table 2 Table 3 Table 4a Table 4b Table 4c Table 4d Table 4e Table 4f Table 4g Table 4h The ability of the compounds of the invention to inhibit neuraminidase in vitro can be determined according to the method described below.

Neuraminidase Inhibition Assay : Influenza virus A/N1/PR/8/34 was grown in the allantoic cavity of fertilized eggs and purified by sucrose density gradient centrifugation (Laver, W. G. (1969) in"Fundamental Techniques in Virology" (K. Habel and N. P. Salzman, eds.) pp.

92-86, Academic Press, New York). Influenza virus A/N2/Tokyo/3/67 was obtained from the tissue culture supernatents of virus grown on MDCK cells.

Neuraminidase from B/Memphis/3/89 virus was prepared by digestion of the virus with TPCK-trypsin followed by centrifugation and then purification of the neuraminidase catalytic fragment using sucrose density gradient centrifugation and dialysis as described previously (Air, G. M., Laver, W. G., Luo, M., Stray, S. J., Legrone, G., and Webster, R. G. (1990) Virole 177, 578-587).

The neuraminidase inhibition assays used the neuraminidase enzymatic activity associated with the A/N1/PR/8/34 or A/N2/Tokyo/3/67 whole virus, or the B/Memphis/3/89 catalytic head fragment. The whole virus or catalytic fragment was diluted appropriately with 20 mM N-ethylmorpholine, 10 mM calcium choride, pH 7.5 buffer on the day of the experiment. Neuraminidase inhibition assays were conducted in 20 mM N-ethylmorpholine, 10 mM calcium choride, pH 7.5 buffer with 5% DMSO. Reaction mixtures included neuraminidase, inhibitor (test compound) and 20-30, uM 4-methylumbelliferyl sialic acid substrate in a total volume of 200 uL and were contained in white 96-well U-shaped plates. Typically, five to eight concentrations of inhibitor were used for each Ki value measurement.

The reactions were initiated by the addition of enzyme and allowed to proceed for 30-60 minutes at room temperature. The fluorescence for each well of the plate was measured once each minute during the reaction period by a Fluoroskan II plate reader (ICN Biomedical) equipped with excitation and emission filters of 355 +/-35 nm and 460 +/-25 nm, respectively. The plate reader was under the control of DeltaSoft II software (Biometallics) and a Macintosh computer. If the compound exhibited linear reaction velocities during the reaction period, then the reaction velocities for the dose-response study were fit to equation 1 using a nonlinear regression program (Kaleidagraph) to determine the overall Ki value (Segel, H. (1975) in Enzyme Kinetics, pp. 105-106, Wiley-Interscience, New York).

(1-ViNo) = [I]/ { [I] + Ki (1 + [S]/Km)} eqn 1 In equation 1, Vi and Vo represent inhibited and uninhibited reaction velocities, respectively, and Km = 16-40, uM depending on the neuraminidase strain tested. For those compounds exhibiting slow-binding inhibition (Morrison, J. F.

(1982) Trends Biochem. Sci. 7, 102-105), a second experiment was performed in a manner identical to the first except that neuraminidase and inhibitor were preincubated in the absence of substrate for 2 hours at room temperature prior to initiating the reactions with substrate. Data analysis for the resulting linear velocities was conducted as described above.

Equation 2 was used to measure Ki values in the sub-nanomolar range (Morrison, J. F. And Stone, S. R. (1985) Comments Mol. Cell Biophys. 2,347- 368).

V = A {sqrt {(Ki'+ It-Et) ^2 + 4Ki'Et}- (Ki'+ It-Et)] eqn. 2 In equation 2, V = velocity ; A = akcat [S]/2 (Km + [S]) ; a is a factor to convert fluorescence units to molar concentrations; Ki'= Ki (1 + [S]/Km) ; It = total inhibitor concentration and Et = total active concentration of neuraminidase.

The compounds of the invention inhibit influenza A neuraminidase and influenza B neuraminidase with Kj values between about 0.1 nanomolar and about 500 micromolar. Preferred compounds of the invention invention inhibit influenza A neuraminidase and influenza B neuraminidase with Kj values between about 0.1 nanomolar and about 3.5 micromolar.

The ability of the compounds of the invention to inhibit plaque formation in cell culture can be determined by the method described below.

Cell Culture Plaque Formation Inhibition Assay Cell Cultures : MDCK cells obtained from the American Type Culture Collection were grown in Dulbecco's Modified Eagle Medium (DMEM) high glucose (GibcoBRL) supplemented with 10% fetal calf serum (JRH Biosciences), 40 mM HEPES buffer (GibcoBRL) and antibiotics (GibcoBRL). Cells were routinely cultured in flasks or roller bottles at 37°C and 5% C02. At confluence cells were reduced to a density of 500,000 cells in a ml using trypsin/EDTA (GibcoBRL) treatment of the monolayer followed by cell centrifugation, resuspension, and dilution into growth media. Cells were planted at a volume to surface area ratio of 1 ml over 1 cm2 of growth surface.

P)aqueAssayProtoco!:OnMDGKce)iconf)uent6we)fptates growth media was removed and the cells were overlaid with 1.5 ml of assay media (DMEM with 1 % fetal calf serum, 40 mM HEPES buffer and antibiotics) containing pre-mixed virus (influenza A/Tokyo/3/67 [H2N2]) (40-100 plaque forming units) and 2x concentration test compound. The plates were placed on a rocker and incubated for 2 hours at room temperature. During the virus adsorption period agar overlay media was prepared. In a microwave oven 2X agarose (final concentration of 0.6% agarose) in overlay media (DMEM with 40 mM HEPES buffer) was melted and then placed in a 48°C water bath for temperature equilibration. After the virus adsorption period was completed 1.5 ml agar over media was added and mixed with the 1.5 ml virus and test compound containing media per well.

Cultures were incubated at 35°C for the period required for plaque development, usually several days. Plaques were fixed with 3.7% formalin in PBS for 20 minutes followed by removal of the agar overlay and staining with 0.1 % crystal violet in distille water for 15 minutes. Plaques were counted and EC 50 concentration determined from multiple concentrations of the tested compound using regression analysis.

Viral Stocks: Stocks were prepared in MDCK confluent roller bottles incubated at 37°C in DMEM supplemented with 1 % FCS, 40mM HEPES buffer, and antibiotics. Bottles were inoculated with a multiplicity of infection of approximately 0.1 plaque forming unit for each cell. Roller bottles were harvested after the cytopathic effect of the virus was observed to be complete. Stocks were prepared from the supernatant resulting from the low speed centrifugation of the media and cell lysate. Stocks were titered and stored at-80°C.

Compounds of the invention provided plaque formation inhibition for influenza virus A/N2/Tokyo in MDCK cells with EC50 values between about 100 micromolar and about 1 nanomolar. Preferred compounds of the invention provided plaque formation inhibition for influenza virus A/N2/Tokyo in MDCK cells with EC50 values between about 1 micromolar and about 1 nanomolar.

The, compounds of the invention can be tested for in vivo antiviral activity using the method described below.

In Vivo Antiviral Efficacy Method Female BALB/c mice were placed under anesthesia (sevoflurane) and inoculated intranasally (IN) with 0.1 ml of influenza A VR-95 (Puerto Rico PR8- 34) at 10-2 (diluted from frozen stock). This viral concentration consistently produced disease in mice within 5 days of inoculation. Animals were treated 4h. pre-infection and 4h. post-infection, andperiodically thereafter, with one of the following therapies: no treatment; test compound (100, 25,6.25,1.39 mg/kg/day BID, PO); or vehicle (sterile water BID, PO). A group of ten animals (designated as control) was inoculated with 0.9% saline. Percent survival was determined.

On day five, lungs were harvested, weighed and assigned scores of 0,1,2,3 or 4 based on percentage consolidation (0; 10-20; 25-50; 50-75; 75-100%, respectively). In addition, each lung pair was image analyzed to determine objective lung consolidation percentages.

The compounds of the present invention can be used in the form of salts derived from inorganic or organic acids. These salts include but are not limited to the following : acetate, trifluoroacetate, adipate, alginate, citrate, aspartate, benzoate, benzenesulfonate, bisulfate, butyrate, camphorate, camphorsulfonate, digluconate, cyclopentanepropionate, dodecylsulfate, ethanesulfonate, glucoheptanoate, glycerophosphate, hemisulfate, heptanoate, hexanoate, fumarate, hydrochloride, hydrobromide, hydroiodide, 2-hydroxy-ethanesulfonate (isethionate), lactate, maleate, methanesulfonate, nicotinate, 2-naphthalenesulfonate, oxalate, pamoate, pectinate, persulfate, 3-phenylpropionate, picrate, pivalate, propionate, succinate, tartrate, thiocyanate, p-toluenesulfonate and undecanoate. Also, basic nitrogen-containing groups can be quaternized with-such agents as lower-alkyl-halides,-such as methyl, ethyl, propyl, and butyl chloride, bromides, and iodides; dialkyl sulfates like dimethyl, diethyl, dibutyl, and diamyl sulfates, long chain halides such as decyl, lauryl, myristyl and stearyl chlorides, bromides and iodides, aralkyl halides like benzyl and phenethyl bromides, and others. Water or oil-soluble or dispersible products are thereby obtained.

Examples of acids which may be employed to form pharmaceutically acceptable acid addition salts include such inorganic acids as hydrochloric acid, hydrobromic acid, sulfuric acid and phosphoric acid and such organic acids as oxalic acid, maleic acid, succinic acid and citric acid. Other salts include salts with alkali metals or alkaline earth metals, such as sodium, potassium, lithium, calcium or magnesium or with ammonium or N (R**) 4+ salts (where R** is loweralkyl).

In addition, salts of the compounds of this invention with one of the naturally occurring amino acids are also contemplated.

Preferred salts of the compounds of the invention include hydrochloride, methanesulfonate, p-toluenesulfonate, sulfonate, phosphonate and isethionate.

The compounds of the formula 1, IIA, IIB, IIIA or IIIB of this invention can have a substituent which is an acid group (for example,-C02H,-S03H,-S02H, -P03H2,-P02H). Compounds of the formula 1, IIA, IIB, IIIA or IIIB of this invention having a substituent which is an ester of such an acidic group are also encompassed by this invention. Such esters may serve as prodrugs. The prodrugs of this invention are metabolized in vivo to provide the above-mentioned acidic substituent of the parental compound of formula 1, IIA, IIB, IIIA or IIIB.

Prodrugs may also serve to increase the solubility of these substances and/or absorption from the gastrointestinal tract. These prodrugs may also serve to increase solubility for intravenous administration of the compounds. Prodrugs may also serve to increase the hydrophobicity of the compounds. Prodrugs may also serve to increase the oral bioavailability of the compounds by increasing absorption and/or decreasing first-pass metabolism. Prodrugs may also serve to increase tissue penetration of the compounds, thereby leading to increased activity in infected tissues and/or reduced rate of clearance.

Such esters contemplated by this invention include: alkyl esters, especially loweralkyl esters, including, but not limited to, ethyl, n-propyl, isopropyl, n-butyl, sec-butyl, isobutyl, t-butyl, n-pentyl esters and the like ; alkoxyalkyl esters, especially, loweralkoxyloweralkyl esters, including, but not limited to, methoxymethyl, 1-ethoxyethyl, 2-methoxyethyl, isopropoxymethyl, t-butoxymethyl esters and the like ; alkoxyalkoxyalkyl esters, especially, alkoxyalkoxy-substituted loweralkyl esters, including, but not limited to, 2-methoxyethoxymethyl esters and the like ; aryloxyalkyl esters, especially, aryloxy-substituted loweralkyl esters, including, but not limited to, phenoxymethyl esters and the like, wherein the aryl group is unsubstituted or substituted as previously defined herein; haloalkoxyalkyl esters, especially, haloalkoxy-substituted loweralkyl esters, including, but not limited to, 2,2,2-trichloroethoxymethyl esters and the like ; alkoxycarbonylalkyl esters, especially, loweralkoxycarbonyl-substituted loweralkyl esters, including, but not limited to, methoxycarbonylmethyl esters and the like ; cyanoalkyl esters, especially, cyano-substituted loweralkyl esters, including, but not limited to, cyanomethyl, 2-cyanoethyl esters and the like ; thioalkoxymethyl esters, especially, lowerthioalkoxy-substituted methyl esters, including, but not limited to, methylthiomethyl, ethylthiomethyl esters and the like ; alkylsulfonylalkyl esters, especially, loweralkylsulfonyl-substituted loweralkyl esters, including, but not limited to, 2-methanesulfonylethyl esters and the like ; arylsulfonylalkyl esters, especially, arylsulfonyl-substituted loweralkyl esters, including, but not limited to, 2-benzenesulfonylethyl and 2- toluenesulfonylethyl esters and the like ; acyloxyalkyl esters, especially, loweralkylacyloxy-substituted loweralkyl esters, including, but not limited to, formyloxymethyl, acetoxymethyl, pivaloyloxymethyl, acetoxyethyl, pivaloyloxyethyl esters and the like ; cycloalkylcarbonyloxyalkyl esters including, but not limited to, cyclopentanecarbonyloxymethyl, cyclohexanecarbonyloxymethyl, cyclopentanecarbonyloxyethyl, cyclohexanecarbonyloxyethyl esters and the like ; arylcarbonyloxyalkyl esters including, but not limited to, benzoyloxymethyl esters and the like ; (alkoxycarbonyloxy) alkyl esters, especially, (loweralkoxycarbonyloxy)- substituted loweralkyl esters, including, but not limited to, methoxycarbonyloxymethyl, ethoxycarbonyloxymethyl, 1- (methoxycarbonyloxy) ethyl, 2- (ethoxycarbonyloxy) ethyl esters and the like ; (cycloalkyloxycarbonyloxy) alkyl esters, especially, (cycloalkyloxycarbonyloxy)-substituted loweralkyl esters, including, but not limited to, cyclohexyloxycarbonyloxymethyl, cyclopentyloxycarbonyloxyethyl, cyclohexyloxycarbonyloxypropyl esters and the like ; oxodioxolenylmethyl esters including, but not limited to, (5-phenyl-2-oxo- 1,3-dioxolen-4-yl) methyl, [5- (4-methylphenyl)-2-oxo-1, 3-dioxolen-4-yl] methyl, [5- (4-methoxyphenyl)-2-oxo-1, 3-dioxolen-4-yl] methyl, [5- (4-fluorophenyl)-2-oxo-1, 3- dioxolen-4-yl] methyl, [5- (4-chlorophenyl)-2-oxo-1, 3-dioxolen-4-yl] methyl, (2-oxo- 1,3-dioxolen-4-yl) methyl, (5-methyl-2-oxo-1, 3-dioxolen-4-yl) methyl, (5-ethyl-2- oxo-1,3-dioxolen-4-yl) methyl, (5-propyl-2-oxo-1, 3-dioxolen-4-yl) methyl, (5- isopropyl-2-oxo-1, 3-dioxolen-4-yl) methyl, (5-butyl-2-oxo-1, 3-dioxolen-4-yl) methyl esters and the like ; phthalidyl esters wherein the phenyl ring of the phthalidyl group is unsubstituted or substituted as defined previously herein, including, but not limited to, phthalidyl, dimethylphthalidyl, dimethoxyphthalidyl esters and the like ; aryl esters including, but not limited to, phenyl, naphthyl, indanyl esters and the like ; arylalkyl esters, especially, aryl-substitued loweralkyl esters, including, but not limited to, benzyl, phenethyl, 3-phenylpropyl, naphthylmethyl esters and the like, wherein the aryl part of the arylalkyl group is unsubstituted or substituted as previously defined herein; dialkylaminoalkyl esters, especially dialkylamino-substituted loweralkyl esters, including, but not limited to, 2- (N, N-dimethylamino) ethyl, 2- (N, N- diethylamino) ethyl ester and the like (heterocyclic) alkyl esters, especially, heterocyclic-substituted loweralkyl esters wherein the heterocycle is a nitrogen-containing heterocycle, including, but not limited to, (heterocyclic) methyl esters and the like, wherein the heterocyclic part of the (heterocyclic) alkyl group is unsubstituted or substituted as previously defined herein; and carboxyalkyl esters, especially, carboxy-substituted loweralkyl esters, including, but not limited to-carboxymethyl-esters and the) ike ; and the like.

Preferred prodrug esters of acid-containing compounds of the Formula I, IIA, IIB, IIIA or IIIB are loweralkyl esters, including, but not limited to, ethyl, n-propyl, isopropyl, n-butyl, sec-butyl, isobutyl, t-butyl, n-pentyl esters, 3-pentyl esters, cycloalkyl esters, cycloalkylalkyl esters and benzyl esters wherein the phenyl ring is unsubstituted or substituted as previously defined herein.

Methods for the preparation of prodrug esters of compounds of the Formula I, IIA, IIB, IIIA or IIIB are well-known in the art and include : reacting the acid with the corresponding halide (for example, chloride or acyl chloride) and a base (for example, triethylamine, DBU, N, N- dimethylaminopyridine and the like) in an inert solvent (for example, DMF, acetonitrile, N-methylpyrrolidone and the like) ; reacting an activated derivative of the acid (for example, an acid chloride, sulfonyl chloride, monochlorophosphonate and the like) with the corresponding alcohol or alkoxide salt ; and the like.

Other examples of prodrugs of the present invention include amides derived from the substituent which is an acid group.

Such amides contemplated by this invention include : simple amides, such as-C (O) NH2 and the like ; alkylamino amides, especially, loweralkylamino amides, including, but not limited to, methylamino, ethylamino, n-propylamino, isopropylamino amides and the like ; cylcoalkylamino amides, including, but not limited to, cylopropylamino, cylcobutylamino, cyclopentylamino, cyclohexylamino amides and the like ; acylamino amides, including, but not limited to acetylamino, propionylamino, butanoylamino amides and the like ; cylcoalkylcarbonylamino amides, including, but not limited to, cyclopropylcarbonylamino, cyclobutylcarbonylamino amides and the like ; alkoxycarbonylalkylamino amides, including, but not limited to, ethoxycarbonylmethylamino, t-butyloxycarbonylmethylamino and the like ; aminoacylamino amides, including, but not limited to, aminoacetylamino amides and the like ; dialkylaminoacylamino amides, including, but not limited to, dimethylaminoacetylamino, diethylaminoacetylamino amides and the like ; (heterocyclic) acylamino amides, including, but not limited to, piperidin-1- ylacetylamino amides and the like ; amides derived from single naturally occuring L-amino acids (or from acid- protected L-amino acids, for example, esters of such amino acids and the like) or from dipeptides comprising two naturally occuring L-amino acids wherein each of the two amino acids is the same or is different (or from acid-protected dipeptides, for example, esters of such dipeptides and the like) ; and the like.

Methods for preparation of prodrug amides of compounds of the invention are well-known in the art and include reacting the acid with the appropriate amine in the presence of an amide bond or peptide bond-forming coupling reagent or reacting an activated derivative of the acid with the appropriate amine and the like.

Other examples of prodrugs of the present invention include esters of hydroxyl-substituted compounds of formula 1, IIA, IIB, IIIA or IIIB which have been acylated with a blocked or unblocked amino acid residue, a phosphate function, a hemisuccinate residue, an acyl residue of the formula R100C (O)-or R'°°C (S)-wherein R'°° is hydrogen, lower alkyl, haloalkyl, alkoxy, thioalkoxy, alkoxyalkyl, thioalkoxyalkyl or haloalkoxy, or an acyl residue of the formula Ra-C (Rb) (Rd)-C (O)- or Ra-C (Rb) (Rd)-C (S)- wherein Rb and Rd are independently selected from hydrogen or lower alkyl and Ra is-N (Re) (R),-ORe or-SRe wherein Re and Rf are independently selected from hydrogen, lower alkyl and haloalkyl, or an amino-acyl residue having the formula R'°'NH (CH2) 2NHCH2C (O)- or R°NH (CH2) 20CH2C (O)-wherein R101 is hydrogen, lower alkyl, (aryl) alkyl, (cycloalkyl) alkyl, acyl, benzoyl or an a-amino acyl group. The amino acid esters of particular interest are of glycine and lysine ; however, other amino acid residues can also be used, including any of the naturally occuring amino acids and also including those wherein the amino acyl group is-C (O) CH2NR102R103 wherein R'02 and R'03 are independently selected from hydrogen and lower alkyl, or the group -NR'R', where R'and R', taken together, forms a nitrogen containing heterocyclic ring.

Other prodrugs include a hydroxyl-substituted compound of formula 1, IIA, IIB, IIIA or IIIB wherein the hydroxyl group is functionalized with a substituent of the formula-CH (R'04) OC (O) R'05 or-CH (R104) OC (S) R'05 wherein Rios is lower alkyl, haloalkyl, alkoxy, thioalkoxy or haloalkoxy and R'04 is hydrogen, lower alkyl, haloalkyl, alkoxycarbonyl, aminocarbonyl, alkylaminocarbonyl or dialkylaminocarbonyl. Such prodrugs can be prepared according to the procedure of Schreiber (Tetrahedron Lett. 1983, 24,2363) by ozonolysis of the corresponding methallyl ether in methanol followed by treatment with acetic anhydride.

The preparation of esters of hydroxyl-substituted compounds of formula formula 1, IIA, IIB, IIIA or IIIB is carried out by reacting a hydroxyl-substituted compound of formula formula 1, IIA, IIB, IIIA or IIIB, with an activated amino acyl, phosphoryl, hemisuccinyl or acyl derivative.

Prodrugs of hydroxyl-substituted-compounds of the invention can also be prepared by alkylation of the hydroxyl substituted compound of formula formula 1, IIA, IIB, IIIA or IIIB, with (halo) alkyl esters, transacetalization with bis- (alkanoyl) acetals or condensation of the hydroxyl group with an activated aldehyde followed by acylation of the intermediate hemiaceta!.

In preparing prodrugs it often is necessary to protect other reactive functional groups, in order to prevent unwanted side reactions. After protection of the reactive groups the desired group can be functionalized. The resulting functionalized product is then deprotected, to remove the protecting groups that were added to prevent unwanted side reactions. This will provide the desired prodrug. Suitable reaction conditions for preparing protecting groups are well known in the art. One source for reaction conditions is found in T. H. Greene and P. G. M. Wuts, Protective Groups in Organic Synthesis, 2nd edition, John Wiley & Sons, New York (1991).

This invention also encompasses compounds of the Formula I, IIA, IIB, IIIA or IIIB which are esters or prodrugs and which are also salts. For example, a compound of the invention can be an ester of a carboxylic acid and also an acid addition salt of an amine or nitrogen-containing substituent in the same compound.

The compounds of the present invention are useful for inhibiting neuraminidase from disease-causing microorganisms which comprise a neuraminidase. The compounds of the invention are useful (in humans, other mammals and fowl) for treating or preventing diseases caused by microorganisms which comprise a neuraminidase.

The compounds of the present invention are useful for inhibiting influenza A virus neuraminidase and influenza B virus neuraminidase, in vitro or in vivo (especially in mammals and, in particular, in humans). The compounds of the present invention are also useful for the inhibition of influenza viruses, orthomyxoviruses, and paramyxoviruses in vivo, especially the inhibition of influenza A viruses and influenza B viruses in humans and other mammals. The compounds of the present invention are also useful for the treatment of infections caused by influenza viruses, orthomyxoviruses, and paramyxoviruses in vivo, especially the human diseases caused by influenza A and influenza B viruses.

The compounds of the present invention are also useful for the prophylaxis of infections caused by influenza viruses, orthomyxoviruses, and paramyxoviruses in vivo in humans and other mammals, especially the prophylaxis of influenza A and influenza B viral infections; and, in particular, the prophylaxis of influenza A and influenza B viral infections in human subjects who are at high risk of developing other respiratory diseases concurrent with or as a consequence of influenza virus infections, or who suffer from chronic respiratory illness, such as asthma, emphysema, or cystic fibrosis.

Total daily dose administered to a human or other mammal host in single or divided doses may be in amounts, for example, from 0.001 to 300 mg/kg body weight daily and more usually 0.1 to 10 mg/kg body weight daily. Dosage unit compositions may contain such amounts of submultiples thereof to make up the daily dose.

The amount of active ingredient that may be combined with the carrier materials to produce a single dosage form will vary depending upon the host treated and the particular mode of administration.

It will be understood, however, that the specific dose level for any particular patient will depend upon a variety of factors including the activity of the specific compound employed, the age, body weight, general health, sex, diet, time of administration, route of administration, rate of excretion, drug combination, and the severity of the particular disease undergoing therapy.

Administration of a compound of this invention will begin before or at the time of infection or after the appearance of established symptoms and/or the confirmation of infection.

The compounds of the present invention may be administered orally, parenterally, sublingually, intranasally, by intrapulmonary administration, by inhalation or insufflation as a solution, suspension or dry powder (for example, in a spray), or rectally, in dosage unit formulations containing conventional nontoxic pharmaceutically acceptable carriers, adjuvants, and vehicles as desired. The term parenteral as used herein includes subcutaneous injections, intravenous, intramuscular, intrasternal injection, or infusion techniques.

Injectable preparations, for example, sterile injectable aqueous or oleagenous suspensions may be formulated according to the known art using suitable dispersing or wetting agents and suspending agents. The sterile injectable preparation may also be a sterile injectable solution or suspension in a nontoxic parenterally acceptable diluent or solvent, for example, as a solution in 1,3-propanediol. Among the acceptable vehicles and solvents that may be employed are water, Ringer's solution, and isotonic sodium chloride solution. In addition, sterile, fixed oils are conventionally employed as a solvent or suspending medium. For this purpose any bland fixed oil may be employed including synthetic mono-or diglycerides. In addition, fatty acids such as oleic acid find use in the preparation of injectables.

Suppositories for rectal administration of the drug can be prepared by mixing the drug with a suitable nonirritating excipient such as cocoa butter and polyethylene glycols which are solid at ordinary temperatures but liquid at the rectal temperature and will therefore melt in the rectum and release the drug.

Solid dosage forms for oral administration may include capsules, tablets, pills, powders, and granules. In such solid dosage forms, the. active compound may be admixed with at least one inert diluent such as sucrose lactose or starch.

Such dosage forms may also comprise, as is normal practice, additional substances other than inert diluents, e. g., lubricating agents such as magnesium stearate. In the case of capsules, tables, and pills, the dosage forms may also comprise buffering agents. Tablets and pills can additionally be prepared with enteric coatings.

Liquid dosage forms for oral administration may include pharmaceutically acceptable emulsions, solutions, suspensions, syrups, and elixirs containing inert diluents commonly used in the art, such as water. Such compositions may also comprise adjuvants, such as wetting agents, emulsifying and suspending agents, and sweetening, flavoring, and perfuming agents.

The compounds of the present invention can also be administered in the form of liposomes. As is known in the art, liposomes are generally derived from phospholipids or other lipid substances. Liposomes are formed by mono-or multi-lamellar hydrated liquid crystals that are dispersed in an aqueous medium.

Any non-toxic, physiologically acceptable and metabolizable lipid capable of forming liposomes can be used. The present compositions in liposome form can contain, in addition to a compound of the present invention, stabilizers, preservatives, excipients, and the like. The preferred lipids are the phospholipids and phosphatidyl cholines (lecithins), both natural and synthetic.

Methods to form liposomes are known in the art. See, for example, Prescott, Ed., Methods in Cell Biology, Volume XIV, Academic Press, New York, N. Y. (1976), p. 33 et seq.

While the compounds of the invention can be administered as the sole active pharmaceutical agent, they can also be used in combination with one or more anti-infective agents and/or other agents used to treat other acute or chronic respiratory ailments. Other agents to be administered in combination with a compound of the present invention include : an influenza vaccine; other influenza inhibitors such as, for example, amantadine, rimantadine, ribavirin, and the like ; another influenza neuraminidase inhibitor, such as, for example, zanamivir or GS 4104 and the like ; agents used to treat respiratory bacterial infections and bronchitis, such as, for example, erythromycin, clarithromycin, azithromycin and the like ; and agents used to treat asthma, such as, for example, zileuton, albuterol (salbutamol), salmeterol, formoterol, ipratropium bromide, inhaled steroids and the like, or anti-inflammatory agents for treating asthma such as, for example, beclomethasone dipropionate, fluticasone propionate, budesonide, triamcinolone acetonide, flunisolide, cromolyn, zafirlukast, montelukast used in combination with a compound of the present invention.

When administered as a combination, the therapeutic agents can be formulated as separate compositions which are given at the same time or different times, or the therapeutic agents can be given as a single composition.

The foregoing is merely illustrative of the invention and is not intended to limit the invention to the disclosed compounds. Variations and changes which are obvious to one skilled in the art are intended to be within the scope and nature of the invention which are defined in the appended claims.