Title:
PYRROLYSYL-TRNA SYNTHETASE
Document Type and Number:
WIPO Patent Application WO/2020/045656
Kind Code:
A1
Abstract:
Obtained is: a method for efficiently producing a polypeptide containing a non-naturally-occurring amino acid; a method for introducing a non-naturally-occurring amino acid into a polypeptide; a method for producing tRNA having a non-naturally-occurring amino acid bonded thereto; a non-naturally-occurring amino acid introduction system; a material for use in these methods; or the like. A method for producing a polypeptide containing a non-naturally-occurring amino acid, which includes a step of bringing PylRS from an organism belonging to the order Methanomassiliicoccales or Thermoplasmatales into contact with the non-naturally-occurring amino acid, is used, the method including an introduction step selected from (a) a step of introducing the non-naturally-occurring amino acid into the polypeptide with higher efficiency compared with the case where a cell-free protein synthesis system using PylRS from Methanosarcina mazei (MmPylRS) is used or (b) a step of introducing the non-naturally-occurring amino acid into the polypeptide with higher efficiency compared with the case where an Escherichia coli protein synthesis system using a vector carrying a gene for the PylRS that is under the regulation by a glmS promoter is used and the case where an Escherichia coli protein synthesis system using a vector carrying a gene for the PylRS that is under the regulation by a glnS promoter is used. Alternatively, a non-naturally-occurring amino acid introduction system containing PylRS from an organism belonging to the order Methanomassiliicoccales or Thermoplasmatales at a high concentration is used.
Inventors:
SAKAMOTO KENSAKU (JP)
YAMAGUCHI ATSUSHI (JP)
KIMURA FUMIE (JP)
YOKOYAMA SHIGEYUKI (JP)
YANAGISAWA TATSUO (JP)
SEKI EIKO (JP)
KURATANI MITSUO (JP)
YAMAGUCHI ATSUSHI (JP)
KIMURA FUMIE (JP)
YOKOYAMA SHIGEYUKI (JP)
YANAGISAWA TATSUO (JP)
SEKI EIKO (JP)
KURATANI MITSUO (JP)
Application Number:
PCT/JP2019/034233
Publication Date:
March 05, 2020
Filing Date:
August 30, 2019
Export Citation:
Assignee:
RIKEN (JP)
International Classes:
C12P21/00; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N15/52; C12P21/02; C12N9/00
Domestic Patent References:
| WO2010083148A1 | 2010-07-22 | |||
| WO2009038195A1 | 2009-03-26 | |||
| WO2009066761A1 | 2009-05-28 | |||
| WO2009038195A1 | 2009-03-26 | |||
| WO2017030156A1 | 2017-02-23 |
Foreign References:
| JP2016534099A | 2016-11-04 | |||
| JP2012514979A | 2012-07-05 |
Other References:
WILLIS JULIAN C.; CHIN JASON W.: "Mutually orthogonal pyrrolysyl-tRNA synthetase/tRNA pairs", NATURE CHEMISTRY, vol. 10, no. 8, 28 May 2018 (2018-05-28), pages 831 - 837, XP036551735, ISSN: 1755-4330, DOI: 10.1038/s41557-018-0052-5
TAKASHI KANAMORI, TAKASHI NAGAIKE, YUTETSU KURUMA, KAZUAKI AMIKURA, TAKUYA UEDA: "Cell -free translation system : Development in biochemistry and advance in synthetic biology", JOURNAL OF JAPANESE BIOCHEMICAL SOCIETY, vol. 89, no. 2, 1 April 2017 (2017-04-01), pages 211 - 220, XP009526579
HOHSAKA TAKAHIRO : "Incorporation of nonnatural amino acids into proteins in cell -free translation systems", JOURNAL OF JAPANESE BIOCHEMICAL SOCIETY, vol. 79, no. 3, 1 March 2007 (2007-03-01), pages 247 - 253, XP009526581, ISSN: 0037-1017
ATSUSHI YAMAGUCHI, FUMIE IRAHA, KAZUMASA OHTAKE, KENSAKU SAKAMOTO: "Pyrrolysyl-tRNA synthetase with a unique architecture enhances the availability of lysine derivatives in synthetic genetic codes", MOLECULES, vol. 23, no. 10, 2460, 26 September 2018 (2018-09-26), pages 1 - 14, XP055695386, DOI: 10.3390/molecules23102460
HERRING ET AL.: "The amino-terminal domain of pyrrolysyl-tRNA synthetase is dispensable in vitro but required for in vivo activity", FEBS LETT, vol. 581, no. 17, 10 July 2007 (2007-07-10), pages 3197 - 203, XP022133714, DOI: 10.1016/j.febslet.2007.06.004
JIANG ET AL.: "PylSn and the Homologous N-terminal Domain of Pyrrolysyl-tRNA Synthetase Bind the tRNA That Is Essential for the Genetic Encoding of Pyrrolysine", J BIOL CHEM, vol. 287, no. 39, 21 September 2012 (2012-09-21), pages 32738 - 32746
SUZUKI ET AL.: "Crystal structures reveal an elusive functional domain of pyrrolysyl-tRNA synthetase", NAT CHEM BIOL, no. 12, 13 December 2017 (2017-12-13), pages 1261 - 1266
WILLIS ET AL.: "Mutually orthogonal pyrrolysyl-tRNA synthetase/tRNA pairs", NAT CHEM, 28 May 2018 (2018-05-28)
"Plumbridge and Soil", BIOCHIMIE, vol. 9, no. 5, 6 May 1987 (1987-05-06), pages 539 - 41
AUSUBEL ET AL.: "Current Protocols in Molecular Biology", 1995, WILEY INTERSCIENCE PUBLISHERS
MARK ET AL., PROC NATL ACAD SCI U S A., vol. 1, no. 18, 8 September 1984 (1984-09-08), pages 5662 - 5666
ZOLLER ET AL., NUCLEIC ACIDS RES., vol. 10, no. 20, 25 October 1982 (1982-10-25), pages 6487 - 6500
WANG ET AL., SCIENCE, vol. 224, no. 4656, 29 June 1984 (1984-06-29), pages 1431 - 1433
HOU J ET AL., EXPERT OPIN DRUG DISCOV, no. 6, 7 June 2012 (2012-06-07), pages 489 - 501
BONNET D ET AL., BIOCONJUG CHEM, vol. 17, no. 6, November 2006 (2006-11-01), pages 1618 - 23
YANAGISAWA ET AL., CHEM BIOL., vol. 15, no. 11, 24 November 2008 (2008-11-24), pages 1187 - 97
MUKAI ET AL., BIOCHEM. BIOPHYS. RES. COMMUN., vol. 371, 2008, pages 818 - 822
See also references of EP 3845661A4
TAKASHI KANAMORI, TAKASHI NAGAIKE, YUTETSU KURUMA, KAZUAKI AMIKURA, TAKUYA UEDA: "Cell -free translation system : Development in biochemistry and advance in synthetic biology", JOURNAL OF JAPANESE BIOCHEMICAL SOCIETY, vol. 89, no. 2, 1 April 2017 (2017-04-01), pages 211 - 220, XP009526579
HOHSAKA TAKAHIRO : "Incorporation of nonnatural amino acids into proteins in cell -free translation systems", JOURNAL OF JAPANESE BIOCHEMICAL SOCIETY, vol. 79, no. 3, 1 March 2007 (2007-03-01), pages 247 - 253, XP009526581, ISSN: 0037-1017
ATSUSHI YAMAGUCHI, FUMIE IRAHA, KAZUMASA OHTAKE, KENSAKU SAKAMOTO: "Pyrrolysyl-tRNA synthetase with a unique architecture enhances the availability of lysine derivatives in synthetic genetic codes", MOLECULES, vol. 23, no. 10, 2460, 26 September 2018 (2018-09-26), pages 1 - 14, XP055695386, DOI: 10.3390/molecules23102460
HERRING ET AL.: "The amino-terminal domain of pyrrolysyl-tRNA synthetase is dispensable in vitro but required for in vivo activity", FEBS LETT, vol. 581, no. 17, 10 July 2007 (2007-07-10), pages 3197 - 203, XP022133714, DOI: 10.1016/j.febslet.2007.06.004
JIANG ET AL.: "PylSn and the Homologous N-terminal Domain of Pyrrolysyl-tRNA Synthetase Bind the tRNA That Is Essential for the Genetic Encoding of Pyrrolysine", J BIOL CHEM, vol. 287, no. 39, 21 September 2012 (2012-09-21), pages 32738 - 32746
SUZUKI ET AL.: "Crystal structures reveal an elusive functional domain of pyrrolysyl-tRNA synthetase", NAT CHEM BIOL, no. 12, 13 December 2017 (2017-12-13), pages 1261 - 1266
WILLIS ET AL.: "Mutually orthogonal pyrrolysyl-tRNA synthetase/tRNA pairs", NAT CHEM, 28 May 2018 (2018-05-28)
"Plumbridge and Soil", BIOCHIMIE, vol. 9, no. 5, 6 May 1987 (1987-05-06), pages 539 - 41
AUSUBEL ET AL.: "Current Protocols in Molecular Biology", 1995, WILEY INTERSCIENCE PUBLISHERS
MARK ET AL., PROC NATL ACAD SCI U S A., vol. 1, no. 18, 8 September 1984 (1984-09-08), pages 5662 - 5666
ZOLLER ET AL., NUCLEIC ACIDS RES., vol. 10, no. 20, 25 October 1982 (1982-10-25), pages 6487 - 6500
WANG ET AL., SCIENCE, vol. 224, no. 4656, 29 June 1984 (1984-06-29), pages 1431 - 1433
HOU J ET AL., EXPERT OPIN DRUG DISCOV, no. 6, 7 June 2012 (2012-06-07), pages 489 - 501
BONNET D ET AL., BIOCONJUG CHEM, vol. 17, no. 6, November 2006 (2006-11-01), pages 1618 - 23
YANAGISAWA ET AL., CHEM BIOL., vol. 15, no. 11, 24 November 2008 (2008-11-24), pages 1187 - 97
MUKAI ET AL., BIOCHEM. BIOPHYS. RES. COMMUN., vol. 371, 2008, pages 818 - 822
See also references of EP 3845661A4
Attorney, Agent or Firm:
SK INTELLECTUAL PROPERTY LAW FIRM et al. (JP)
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