Title:
QUANTIFICATION OF A MINORITY NUCLEIC ACID SPECIES
Document Type and Number:
WIPO Patent Application WO/2012/149339
Kind Code:
A3
Abstract:
The technology relates in part to quantification of a minority nucleic acid species from a nucleic acid sample. In some embodiments, methods for determining the amount of fetal nucleic acid (e.g. absolute amount, relative amount) in a maternal sample are provided. The methods employ inhibitory oligonucleotides for reducing the efficiency of the amplification of the dominant nucleic acid and/or target competitors for improving the quantification.
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Inventors:
NYGREN ANDERS (US)
Application Number:
PCT/US2012/035479
Publication Date:
March 14, 2013
Filing Date:
April 27, 2012
Export Citation:
Assignee:
SEQUENOM INC (US)
NYGREN ANDERS (US)
NYGREN ANDERS (US)
International Classes:
C12Q1/68
Domestic Patent References:
| WO2011034631A1 | 2011-03-24 | |||
| WO2002018616A1 | 2002-03-07 |
Foreign References:
| US6057134A | 2000-05-02 |
Other References:
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PILAR SANCHEZ ET AL: "Effects of Sulpiride on Prolactin and mRNA Levels of Steroid 5alpha-reductase Isozymes in Adult Rat Brain", NEUROCHEMICAL RESEARCH, KLUWER ACADEMIC PUBLISHERS-PLENUM PUBLISHERS, NE, vol. 33, no. 5, 17 October 2007 (2007-10-17), pages 820 - 825, XP019579128, ISSN: 1573-6903
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LIU Z ET AL: "Quantification of regional DNA methylation by liquid chromatography/tandem mass spectrometry", ANALYTICAL BIOCHEMISTRY, ACADEMIC PRESS INC, NEW YORK, vol. 391, no. 2, 15 August 2009 (2009-08-15), pages 106 - 113, XP026218819, ISSN: 0003-2697, [retrieved on 20090512], DOI: 10.1016/J.AB.2009.05.012
PILAR SANCHEZ ET AL: "Effects of Sulpiride on Prolactin and mRNA Levels of Steroid 5alpha-reductase Isozymes in Adult Rat Brain", NEUROCHEMICAL RESEARCH, KLUWER ACADEMIC PUBLISHERS-PLENUM PUBLISHERS, NE, vol. 33, no. 5, 17 October 2007 (2007-10-17), pages 820 - 825, XP019579128, ISSN: 1573-6903
KRISTENSEN LASSE SOMMER ET AL: "PCR-Based Methods for Detecting Single-Locus DNA Methylation Biomarkers in Cancer Diagnostics, Prognostics, and Response to Treatment", CLINICAL CHEMISTRY, AMERICAN ASSOCIATION FOR CLINICAL CHEMISTRY, WASHINGTON, DC, vol. 55, no. 8, 1 August 2009 (2009-08-01), pages 1471 - 1483, XP002596712, ISSN: 0009-9147, [retrieved on 20090611], DOI: 10.1373/CLINCHEM.2008.121962
LIU Z ET AL: "Quantification of regional DNA methylation by liquid chromatography/tandem mass spectrometry", ANALYTICAL BIOCHEMISTRY, ACADEMIC PRESS INC, NEW YORK, vol. 391, no. 2, 15 August 2009 (2009-08-15), pages 106 - 113, XP026218819, ISSN: 0003-2697, [retrieved on 20090512], DOI: 10.1016/J.AB.2009.05.012
Attorney, Agent or Firm:
DICKINSON, Kari, A. et al. (PortfoliolpP.O. Box 5205, Minneapolis MN, US)
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