Title:
RECOMBINANT BACTERIUM FOR PRODUCING SHIKIMIC ACID, CONSTRUCTION METHOD THEREFOR, AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/092633
Kind Code:
A1
Abstract:
Provided is a recombinant bacterium for producing shikimic acid, for use in expressing a target gene for regulating asymmetric cell division and target genes for regulating shikimic acid production. The target gene for regulating the asymmetric cell division comprises a gene popZ for coding cytoskeleton protein PopZ, and the target genes for regulating shikimic acid production comprise a gene aroG for coding DAHP synthetase, a gene aroB for coding 3-dehydroquinic acid synthase, and a gene tktA for coding transketolase. The recombinant strain implements de novo synthesis of shikimic acid, a substrate is glucose, the cost is low, and after the strain is fermented in a 7.5 L fermentation tank, the highest yield of shikimic acid is 88.1 g/L, the yield is 0.33 g/g, and the production intensity is 1.1 g/L/h.
Inventors:
LIU LIMING (CN)
DING QIANG (CN)
CHEN XIULAI (CN)
GAO CONG (CN)
GUO LIANG (CN)
HU GUIPENG (CN)
LIU JIA (CN)
DING QIANG (CN)
CHEN XIULAI (CN)
GAO CONG (CN)
GUO LIANG (CN)
HU GUIPENG (CN)
LIU JIA (CN)
Application Number:
PCT/CN2021/135282
Publication Date:
June 01, 2023
Filing Date:
December 03, 2021
Export Citation:
Assignee:
UNIV JIANGNAN (CN)
International Classes:
C12N1/21; C12N15/31; C12N15/54; C12N15/60; C12N15/64; C12N15/70; C12P7/42; C12R1/19
Foreign References:
| US20190322980A1 | 2019-10-24 | |||
| CN102994439A | 2013-03-27 |
Other References:
LIU XIANG-LEI; LIN JUN; HU HAI-FENG; ZHOU BIN; ZHU BAO-QUAN: "Enhanced production of shikimic acid using a multi-gene co-expression system inEscherichia coli", CHINESE JOURNAL OF NATURAL MEDICINES, ELSEVIER, AMSTERDAM, NL, vol. 14, no. 4, 1 January 1900 (1900-01-01), AMSTERDAM, NL, pages 286 - 293, XP029518877, ISSN: 1875-5364, DOI: 10.1016/S1875-5364(16)30029-2
DATABASE PROTEIN ANONYMOUS : "pole-organizing protein popZ [Caulobacter vibrioides NA1000]", XP093068464, retrieved from NCBI
YAN-YAN CUI;CHEN LING;YUAN-YUAN ZHANG;JIAN HUANG;JIAN-ZHONG LIU: "Production of shikimic acid from Escherichia coli through chemically inducible chromosomal evolution and cofactor metabolic engineering", MICROBIAL CELL FACTORIES, SPRINGER, vol. 13, no. 1, 10 February 2014 (2014-02-10), pages 21, XP021175884, ISSN: 1475-2859, DOI: 10.1186/1475-2859-13-21
GAO CONG, HOU JIANSHEN, XU PENG, GUO LIANG, CHEN XIULAI, HU GUIPENG, YE CHAO, EDWARDS HARLEY, CHEN JIAN, CHEN WEI, LIU LIMING: "Programmable biomolecular switches for rewiring flux in Escherichia coli", NATURE COMMUNICATIONS, vol. 10, no. 1, 1 December 2019 (2019-12-01), XP055929815, DOI: 10.1038/s41467-019-11793-7
HOU JIANSHEN, GAO CONG, CHEN XIULAI, LIU LIMING: "Using dynamic molecular switches for shikimic acid production in Escherichia coli", CHINESE JOURNAL OF BIOTECHNOLOGY, vol. 36, no. 10, 25 October 2020 (2020-10-25), pages 2104 - 2112, XP093068595, DOI: 10.13345/j.cjb.200078
DATABASE PROTEIN ANONYMOUS : "pole-organizing protein popZ [Caulobacter vibrioides NA1000]", XP093068464, retrieved from NCBI
YAN-YAN CUI;CHEN LING;YUAN-YUAN ZHANG;JIAN HUANG;JIAN-ZHONG LIU: "Production of shikimic acid from Escherichia coli through chemically inducible chromosomal evolution and cofactor metabolic engineering", MICROBIAL CELL FACTORIES, SPRINGER, vol. 13, no. 1, 10 February 2014 (2014-02-10), pages 21, XP021175884, ISSN: 1475-2859, DOI: 10.1186/1475-2859-13-21
GAO CONG, HOU JIANSHEN, XU PENG, GUO LIANG, CHEN XIULAI, HU GUIPENG, YE CHAO, EDWARDS HARLEY, CHEN JIAN, CHEN WEI, LIU LIMING: "Programmable biomolecular switches for rewiring flux in Escherichia coli", NATURE COMMUNICATIONS, vol. 10, no. 1, 1 December 2019 (2019-12-01), XP055929815, DOI: 10.1038/s41467-019-11793-7
HOU JIANSHEN, GAO CONG, CHEN XIULAI, LIU LIMING: "Using dynamic molecular switches for shikimic acid production in Escherichia coli", CHINESE JOURNAL OF BIOTECHNOLOGY, vol. 36, no. 10, 25 October 2020 (2020-10-25), pages 2104 - 2112, XP093068595, DOI: 10.13345/j.cjb.200078
Attorney, Agent or Firm:
CENTRAL SOUTH WELL INTELLECTUAL PROPERTY OFFICE (CN)
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