Title:
RECOMBINANT MICROALGAE HAVING ENHANCED TOLERANCE TO HIGH CONCENTRATIONS OF CO2 AND/OR ACIDIC CONDITIONS
Document Type and Number:
WIPO Patent Application WO/2022/211562
Kind Code:
A1
Abstract:
The present invention relates to a novel recombinant microalgal strain having enhanced resistance to high concentrations of CO2 and low pH (acidic) conditions and, more particularly, to Chlamydomonas reinhardtii PMA4ΔCter-V4 and -V10, which are Chlamydomonas reinhardtii-derived genetically modified microalgal strains, and use thereof.
Inventors:
SIM SANG JUN (KR)
HWANG SUNG-WON (KR)
CHOI HONG IL (KR)
HWANG SUNG-WON (KR)
CHOI HONG IL (KR)
Application Number:
PCT/KR2022/004680
Publication Date:
October 06, 2022
Filing Date:
April 01, 2022
Export Citation:
Assignee:
UNIV KOREA RES & BUS FOUND (KR)
International Classes:
C12N1/12; C12N9/14; C12N15/79; C12P7/649; C12R1/89
Foreign References:
| KR101273234B1 | 2013-06-11 |
Other References:
SUNG-WON HWANG, JONGRAE KIM, HONG IL CHOI, EONSEON JIN, WON SEOK CHANG, SANG JUN SIM: "Heterologous expression of plant plasma membrane H⁺-ATPase in Chlamydomonas reinhardtii for an enhancement of acid tolerance", KOREAN SOCIETY OF BIOLOGICAL ENGINEERING CONFERENCE, 1 April 2019 (2019-04-01), pages 293, XP009539975
LEFEBVRE BENOIT, BATOKO HENRI, DUBY GEOFFREY, BOUTRY MARC: "Targeting of a Nicotiana plumbaginifolia H + -ATPase to the Plasma Membrane Is Not by Default and Requires Cytosolic Structural Determinants", THE PLANT CELL, AMERICAN SOCIETY OF PLANT BIOLOGISTS, US, vol. 16, no. 7, 1 July 2004 (2004-07-01), US , pages 1772 - 1789, XP055972273, ISSN: 1040-4651, DOI: 10.1105/tpc.022277
MELISSA A. SCRANTON ET AL: "Chlamydomonas as a model for biofuels and bio-products production", THE PLANT JOURNAL, vol. 82, 1 January 2015 (2015-01-01), pages 523 - 531, XP002777304
ZHANG XINXIN, WANG HUI, LIU SHENKUI, TAKANO TETSUO: "Cloning and characterization of a plasma membrane H⁺-ATPase (PMA) gene from a salt-tolerant plant Chloris virgata", MOLECULAR SOIL BIOLOGY, vol. 5, no. 3, 1 January 2014 (2014-01-01), pages 16 - 22, XP009541196, ISSN: 1925-2005, DOI: 10.5376/msb.2014.05.0003
HIROOKA SHUNSUKE, HIROSE YUU, KANESAKI YU, HIGUCHI SUMIO, FUJIWARA TAKAYUKI, ONUMA RYO, ERA ATSUKO, OHBAYASHI RYUDO, UZUKA AKIHIRO: "Acidophilic green algal genome provides insights into adaptation to an acidic environment", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, NATIONAL ACADEMY OF SCIENCES, vol. 114, no. 39, 26 September 2017 (2017-09-26), pages 2017, XP055972283, ISSN: 0027-8424, DOI: 10.1073/pnas.1707072114
CHOI HONG IL, HWANG SUNG-WON, KIM JONGRAE, PARK BYEONGHYEOK, JIN EONSEON, CHOI IN-GEOL, SIM SANG JUN: "Augmented CO2 tolerance by expressing a single H+-pump enables microalgal valorization of industrial flue gas", NATURE COMMUNICATIONS, vol. 12, no. 1, 1 December 2021 (2021-12-01), XP055972289, DOI: 10.1038/s41467-021-26325-5
LEFEBVRE BENOIT, BATOKO HENRI, DUBY GEOFFREY, BOUTRY MARC: "Targeting of a Nicotiana plumbaginifolia H + -ATPase to the Plasma Membrane Is Not by Default and Requires Cytosolic Structural Determinants", THE PLANT CELL, AMERICAN SOCIETY OF PLANT BIOLOGISTS, US, vol. 16, no. 7, 1 July 2004 (2004-07-01), US , pages 1772 - 1789, XP055972273, ISSN: 1040-4651, DOI: 10.1105/tpc.022277
MELISSA A. SCRANTON ET AL: "Chlamydomonas as a model for biofuels and bio-products production", THE PLANT JOURNAL, vol. 82, 1 January 2015 (2015-01-01), pages 523 - 531, XP002777304
ZHANG XINXIN, WANG HUI, LIU SHENKUI, TAKANO TETSUO: "Cloning and characterization of a plasma membrane H⁺-ATPase (PMA) gene from a salt-tolerant plant Chloris virgata", MOLECULAR SOIL BIOLOGY, vol. 5, no. 3, 1 January 2014 (2014-01-01), pages 16 - 22, XP009541196, ISSN: 1925-2005, DOI: 10.5376/msb.2014.05.0003
HIROOKA SHUNSUKE, HIROSE YUU, KANESAKI YU, HIGUCHI SUMIO, FUJIWARA TAKAYUKI, ONUMA RYO, ERA ATSUKO, OHBAYASHI RYUDO, UZUKA AKIHIRO: "Acidophilic green algal genome provides insights into adaptation to an acidic environment", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, NATIONAL ACADEMY OF SCIENCES, vol. 114, no. 39, 26 September 2017 (2017-09-26), pages 2017, XP055972283, ISSN: 0027-8424, DOI: 10.1073/pnas.1707072114
CHOI HONG IL, HWANG SUNG-WON, KIM JONGRAE, PARK BYEONGHYEOK, JIN EONSEON, CHOI IN-GEOL, SIM SANG JUN: "Augmented CO2 tolerance by expressing a single H+-pump enables microalgal valorization of industrial flue gas", NATURE COMMUNICATIONS, vol. 12, no. 1, 1 December 2021 (2021-12-01), XP055972289, DOI: 10.1038/s41467-021-26325-5
Attorney, Agent or Firm:
JUNG, Eun Youl et al. (KR)
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