Title:
RECOMBINANT ORGANISM AND METHOD FOR PRODUCING MYCOSPORINE-LIKE AMINO ACIDS
Document Type and Number:
WIPO Patent Application WO/2024/078367
Kind Code:
A1
Abstract:
A recombinant organism and a method for producing mycosporine-like amino acids. The method comprises: an organism construction step, comprising: providing an organism of which endogenous genes are knocked out, and integrating an xylose metabolic gene into a genome of the organism to obtain an organism capable of producing S7P by using xylose; and a DDGS-OMT construction step, comprising: integrating at least one of a DDGS gene and an OMT gene into a genome of the organism obtained in the organism construction step, to obtain an organism capable of producing 4DG by using xylose. The yield of mycosporine-like amino acids is remarkably increased by knocking out endogenous genes.
Inventors:
WANG BOXIANG (CN)
YU JUN (CN)
YU JUN (CN)
Application Number:
PCT/CN2023/122858
Publication Date:
April 18, 2024
Filing Date:
September 28, 2023
Export Citation:
Assignee:
SHENZHEN LINK SPIDER CO LTD (CN)
International Classes:
C12N15/81; C12N15/31; C12N15/60; C12P13/00
Foreign References:
| CN115851807A | 2023-03-28 | |||
| CN114901631A | 2022-08-12 |
Other References:
PARK, S. H. ET AL.: "Metabolic Engineering of Saccharomyces Cerevisiae for Production of Shinorine, A Sunscreen Material, from Xylose", ACS SYNTH BIOL, vol. 8, no. 2, 26 December 2018 (2018-12-26), pages 346 - 357, XP055633219, DOI: 10.1021/acssynbio.8b00388
LIANG SUN: "Xylose Assimilation for the Efficient Production of Biofuels and Chemicals by Engineered Saccharomyces cerevisiae", BIOTECHNOLOGY JOURNAL, WILEY-VCH VERLAG, WEINHEIM, DE, vol. 16, no. 4, 1 April 2021 (2021-04-01), DE , pages 2000142, XP093158692, ISSN: 1860-6768, DOI: 10.1002/biot.202000142
CHAEYEON JIN: "Efficient production of shinorine, a natural sunscreen material, from glucose and xylose by deleting HXK2 encoding hexokinase in Saccharomyces cerevisiae", FEMS YEAST RESEARCH JUN 2010, vol. 21, no. 7, 12 October 2021 (2021-10-12), pages foab053, XP093158693, ISSN: 1567-1364, DOI: 10.1093/femsyr/foab053
JIAN ZHA: "Yeast-Based Biosynthesis of Natural Products From Xylose", FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, FRONTIERS RESEARCH FOUNDATION, CH, vol. 9, 3 February 2021 (2021-02-03), CH , pages 634919, XP093158695, ISSN: 2296-4185, DOI: 10.3389/fbioe.2021.634919
LIANG SUN: "Xylose Assimilation for the Efficient Production of Biofuels and Chemicals by Engineered Saccharomyces cerevisiae", BIOTECHNOLOGY JOURNAL, WILEY-VCH VERLAG, WEINHEIM, DE, vol. 16, no. 4, 1 April 2021 (2021-04-01), DE , pages 2000142, XP093158692, ISSN: 1860-6768, DOI: 10.1002/biot.202000142
CHAEYEON JIN: "Efficient production of shinorine, a natural sunscreen material, from glucose and xylose by deleting HXK2 encoding hexokinase in Saccharomyces cerevisiae", FEMS YEAST RESEARCH JUN 2010, vol. 21, no. 7, 12 October 2021 (2021-10-12), pages foab053, XP093158693, ISSN: 1567-1364, DOI: 10.1093/femsyr/foab053
JIAN ZHA: "Yeast-Based Biosynthesis of Natural Products From Xylose", FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, FRONTIERS RESEARCH FOUNDATION, CH, vol. 9, 3 February 2021 (2021-02-03), CH , pages 634919, XP093158695, ISSN: 2296-4185, DOI: 10.3389/fbioe.2021.634919
Attorney, Agent or Firm:
DHC IP ATTORNEYS (CN)
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