ROTAVIRUS GENOTYPE DETECTION METHOD, AND GENE AMPLIFICATION PRIMER SET USED IN SAME

WIPO Patent Application WO/2020/071419

A1

The present invention addresses the problem of providing a novel gene detection primer and a detection means which uses the same, whereby a rotavirus-A genotype can be more accurately detected. Provided are a method for detecting the genotype of a rotavirus, a gene amplification primer set used in said method, and a genotype detection kit. In the method for detecting the genotype of a rotavirus: (A) using positions 174-834 in the base sequence of the VP7 gene segment of a G1 type strain of rotavirus A as a base sequence count reference, a sense primer matching each of 1 to 4 base sequence regions selected from (1) positions 802-834 (corresponding to type G3), (2) positions 747-789 (corresponding to equine-like type G3), (3) positions 603-636 (corresponding to type G9), and (4) positions 666-711 (corresponding to type G12) is used as a basic sense primer for gene amplification; and, (B) using positions 883-1062 as a count reference, an antisense primer matching each of the corresponding base sequence regions is used as a shared antisense primer for gene amplification.

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FUJII YOSHIKI (JP)

PCT/JP2019/038893

April 09, 2020

October 02, 2019

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SHIMADZU CORP (JP)
FUJII YOSHIKI (JP)

C12Q1/6888; C12Q1/686; C12N15/09

US20090170066A1

2009-07-02

I TURRIZA-GOMARA, MIREN ET AL.: "Rotavirus genotyping: keeping up with an evolving population of human rotaviruses", JOURNAL OF CLINICAL VIROLOGY, vol. 31, 2004, pages 259 - 265, XP004604795, DOI: 10.1016/j.jcv.2004.04.009
COWLEY, DANIEL ET AL.: "Emergence of a novel equine-like G3P[8] intergenogroup reassortant rotavirus strain associated with gastroenteritis in Australian children", JOURNAL OF GENERAL VIROLOGY, vol. 97, 2016, pages 403 - 410, XP055704404
FUJII, YOSHIKI: "Improvement of Rotavirus Genotyping Method by Using the Semi-Nested Multiplex-PCR With New Primer Set", FRONTIERS IN MICROBIOLOGY, vol. 10, March 2019 (2019-03-01), XP055704405

SHIMURA Mitsuharu (JP)

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