Title:
SECRETED ACID PHOSPHATASE (SAPM) IS PRESENT ONLY IN PATHOGENIC MYCOBACTERIA AND EXPRESSED SELECTIVELY AT ACIDIC PH
Document Type and Number:
WIPO Patent Application WO2004033677
Kind Code:
A3
Abstract:
The invention relates to an isolated DNA construct, comprising a mycobacterium secreted acid phosphatase promoter or promoter fragment which is inducible by low pH. This invention further relates to diagnostic methods and vaccines for treatment or prophylaxis of pathogenic mycobacterial disease or infection. This invention also provides for methods of screening for compounds capable of modulating the activity, production or secretion of a mycobacterium secreted acid phosphatase.
Inventors:
LIU JUN (CA)
SALEH MAZEN T (CA)
ALEXANDER DAVID (CA)
SALEH MAZEN T (CA)
ALEXANDER DAVID (CA)
Application Number:
PCT/CA2003/001554
Publication Date:
June 17, 2004
Filing Date:
October 09, 2003
Export Citation:
Assignee:
LIU JUN (CA)
SALEH MAZEN T (CA)
ALEXANDER DAVID (CA)
SALEH MAZEN T (CA)
ALEXANDER DAVID (CA)
International Classes:
A61K39/04; C07K16/12; C12N9/16; C12N15/52; C12N15/55; C12N15/57; C12N15/63; G01N33/50; A61K38/00; A61K39/00; (IPC1-7): C12N9/16; C12N15/55; C12N15/67; C12N5/10; C07K16/12; G01N33/50; A61K39/04
Domestic Patent References:
| WO2001081422A1 | 2001-11-01 |
Other References:
SALEH MAZEN T ET AL: "Secretion of an acid phosphatase (SapM) by Mycobacterium tuberculosis that is similar to eukaryotic acid phosphatases", JOURNAL OF BACTERIOLOGY, vol. 182, no. 23, December 2000 (2000-12-01), pages 6850 - 6853, XP002275003, ISSN: 0021-9193
DATABASE EMBL [online] 2 September 2002 (2002-09-02), FLEISCHMANN ET AL.: "Mycobacterium tuberculosis CDC1551, section 235 of 280 of the complete genome", XP002275007, Database accession no. AE007149
COLE S T ET AL: "Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence", NATURE, MACMILLAN JOURNALS LTD. LONDON, GB, vol. 393, 11 June 1998 (1998-06-11), pages 537 - 544, XP002087941, ISSN: 0028-0836
TRICCAS JAMES A ET AL: "Life on the inside: Probing Mycobacterium tuberculosis gene expression during infection", IMMUNOLOGY AND CELL BIOLOGY, vol. 78, no. 4, August 2000 (2000-08-01), pages 311 - 317, XP002275004, ISSN: 0818-9641
KUEHNEL M P ET AL: "Characterization of the intracellular survival of Mycobacterium avium ssp. paratuberculosis: phagosomal pH and fusogenicity in J774 macrophages compared with other mycobacteria.", CELLULAR MICROBIOLOGY. ENGLAND AUG 2001, vol. 3, no. 8, August 2001 (2001-08-01), pages 551 - 566, XP002275005, ISSN: 1462-5814
FISHER MARK A ET AL: "Microarray analysis of the Mycobacterium tuberculosis transcriptional response to the acidic conditions found in phagosomes", JOURNAL OF BACTERIOLOGY, vol. 184, no. 14, July 2002 (2002-07-01), pages 4025 - 4032, XP002275006, ISSN: 0021-9193
DATABASE EMBL [online] 2 September 2002 (2002-09-02), FLEISCHMANN ET AL.: "Mycobacterium tuberculosis CDC1551, section 235 of 280 of the complete genome", XP002275007, Database accession no. AE007149
COLE S T ET AL: "Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence", NATURE, MACMILLAN JOURNALS LTD. LONDON, GB, vol. 393, 11 June 1998 (1998-06-11), pages 537 - 544, XP002087941, ISSN: 0028-0836
TRICCAS JAMES A ET AL: "Life on the inside: Probing Mycobacterium tuberculosis gene expression during infection", IMMUNOLOGY AND CELL BIOLOGY, vol. 78, no. 4, August 2000 (2000-08-01), pages 311 - 317, XP002275004, ISSN: 0818-9641
KUEHNEL M P ET AL: "Characterization of the intracellular survival of Mycobacterium avium ssp. paratuberculosis: phagosomal pH and fusogenicity in J774 macrophages compared with other mycobacteria.", CELLULAR MICROBIOLOGY. ENGLAND AUG 2001, vol. 3, no. 8, August 2001 (2001-08-01), pages 551 - 566, XP002275005, ISSN: 1462-5814
FISHER MARK A ET AL: "Microarray analysis of the Mycobacterium tuberculosis transcriptional response to the acidic conditions found in phagosomes", JOURNAL OF BACTERIOLOGY, vol. 184, no. 14, July 2002 (2002-07-01), pages 4025 - 4032, XP002275006, ISSN: 0021-9193
Download PDF:
Previous Patent: METHOD AND MICROORGANISM FOR THE PRODUCTION OF D-MANNITOL
Next Patent: SPLICE-VARIANT OF HUMAN PDE6C
Next Patent: SPLICE-VARIANT OF HUMAN PDE6C