More particularly, this invention is concerned with the use of anesthetics which are ligands for y-aminobutyric acid type A (GABAA) receptors. In particular, this invention is concerned with the use of anesthetics that have a selective action at GABAA receptors containing a (3s subunit. The present invention also provides a transgenic non-human animal and uses thereof.

Receptors for the major inhibitory neurotransmitter, gamma- aminobutyric acid (GABA), are divided into two main classes: (1) GABAA receptors, which are members of the ligand-gated ion channel superfamily ; and (2) CABAs receptors, which are members of the G-protein coupled receptor superfamily. Since the first cDNAs encoding individual GABAA receptor subunits were cloned the number of known members of the mammalian family has grown to include at least six a subunits, three (3 subunits, three y subunits, one 8 subunit, one 0, and one £ subunit. The native GABAA receptor is a pentameric assembly of subunits drawn from these classes. It has been indicated that an oc subunit, a (3 subunit and a y subunit constitute the minimum requirement for forming a fully functional GABAA receptor expressed by transiently transfecting cDNAs into cells. As indicated above, a 0, 8 and E subunit also exist, but these are present only to a minor extent in GABAA receptor populations.

It is now generally recognised that GABAA receptors are an important anesthetic target. At anesthetic or subanesthetic concentrations many structurally diverse general anesthetics act to potentiate GABAA receptor-mediated neuronal responses. Higher concentrations of such agents can elicit direct activation of the receptor in the absence of GABA-see, for instance, D. L. Tanelian et al.,

Anesthesiology, 78 (4), 757-776 (1993); N. P. Franks and W. R. Lieb, Nature, 367,607-614 (1994); and R. A. Harris et al., FASEB J. , 9, 1454-1462 1995).

Potentiation of the action of GABA by most anesthetics shows little in the way of subunit specificity. Thus, for instance, with both native and all recombinant GABAA receptors studied to date, both alphaxalone and propofol markedly potentiate the action of GABA. In contrast, the direct action of etomidate at GABAA receptors is influenced by the type of (3 subunit. The potentiation and direct activation of receptors by etomidate favours receptors containing either a p2 or a ß3 subunit, versus a pi subunit-see also C. Hill-Venning et al., Br. J. Pharmacol., 120,749-756 (1997).

This selectivity of etomidate for (32-or 3s-containing receptors is determined by a single amino acid. The mutation of asparagine at position 289 (which is present within the channel domain of the ß3 subunit) to serine (the homologous residue in pi) strongly suppressed the GABA-modulatory and GABA-mimetic effects of etomidate-see D. Belelli et al., Proc. Natl. Acad. Sci. USA, 94,11031-11036 (1997).

SUMMARY OF THE INVENTION We have now found that a GABAA receptor (3s selective anesthetic agent will have the highly desirable characteristic of affording rapid recovery. A genetically engineered"knock-in"mouse that has the pz subunit mutated at the single key amino acid ( (32N289S) has been f prepared such that etomidate will only be acting on the mice through GABAA receptors containing ß3 subunits. When treated with anesthetic doses of etomidate, these ß2N289S mice show a dramatically faster recovery of performance in the rotarod assay, compared with similarly treated wildtype mice.

BRIEF DESCRIPTION OF FIGURES Figure 1 shows the targeting strategy for the GABAA receptor 02N289S knock-in mice. (a) Schematic representation of the wildtype (32 allele of the GABAA receptor and (b) targeting vector including the site- specific (32N289S mutation indicated by an asterisk. E, EcoRI; K, KpnI restriction sites; 6, 7 and 8, exons 6,7 and 8, respectively; black arrowhead, loxP site; neo, neomycin resistance gene; TK, thymidine kinase gene. (c) Targeted (32 allele after homologous recombination before and (d) after cre-mediated excision of the loxP flanked neomycin cassette.

Figure 2 shows the anesthetic effects of etomidate in (32N289S knock-in mice measured by the loss of the righting reflex.

Figure 3 shows the recovery of wildtype and (32N289S knock-in mice following etomidate-induced anesthesia.

Figure 4 shows the level of anesthesia induced by etomidate as determined by electroencephalography (EEG) in wildtype and (32N289S knock-in mice.

Figure 5 shows the level of"hypnotic hangover"measured as an effect on slow wave sleep following recovery from etomidate anesthesia in wildtype and (32N289S knock-in mice.

DETAILED DESCRIPTION OF THE INVENTION Thus, according to a first aspect of the present invention, there is provided the use of a GABAA receptor 3 selective anesthetic agent for the manufacture of a medicament for providing anesthesia.

There is further provided a method for providing anesthesia, comprising administering to a mammal a GABAA receptor p3 selective anesthetic agent in an amount equal to or in excess of an amount effective for anesthesia in order to induce or maintain anesthesia.

Reference herein to the"GABAA receptor"will be understood to be, most preferably, a reference to the human GABAA receptor.

As used herein, the term"GABAA receptor ß3 selective anesthetic agent"refers to an anesthetic agent which has a selective modulatory action at GABAA receptors containing a ß3 subunit. Thus, compounds of use in the present invention will produce a potent enhancement of the control response evoked by GABA on a GABAA receptor containing a ß3 subunit. Such compounds will ideally have an ECso of <10 yM, and preferably <5 jM. The compounds of use in this invention will possess at least a 2-fold, suitably at least a 5-fold, and advantageously at least a 10- fold, selective affinity for the ps subunit, relative to the pz subunit. The compounds of use in this invention will also preferably show such selectivity relative to the Pi subunit.

The compounds of use in the present invention may also possess an agonist action at the GABAA receptor containing a ß3 subunit, in the absence of GABA. Such compounds will ideally have an ECSO of <25, uM, and preferably < 10, uM. The compounds of use in this invention will possess at least a 2-fold, suitably at least a 5-fold, and advantageously at least a 10-fold, selective agonist activity at the pg subunit, relative to the p2 subunit. The compounds of use in this invention will also preferably show such selectivity relative to the pi subunit.

Therefore, according to another aspect of the present invention, there is provided the use of GABAA receptor pg selective anesthetic agent for the manufacture of a medicament for providing anesthesia, wherein said anesthetic agent is a modulator of the GABAA receptor containing a pg subunit, having potency (EC5o) for such a receptor of 10 uM or less, which elicits at least a 30% potentiation of the GABA EC2o response in transfected L (tk-) cells expressing the GABAA receptor containing the ps subunit, and which elicits at most a 20% potentiation of the GABA ECio response in transfected L (th-) cells expressing the GABAA receptor containing the 02 subunit.

This aspect of the present invention also provides a method for providing anesthesia comprising administering to a mammal a GABAA

receptor Ps functionally selective anesthetic agent in an amount equal to or in excess of an amount effective for anesthesia in order to induce or maintain anesthesia, wherein said anesthetic agent is a modulator of GABAA receptor containing a ß3 subunit, having potency (ECso) for such a receptor of 10 uM or less, which elicits at least a 30% potentiation of the GABA EC20 response in transfected L (tk-) cells expressing the GABAA receptor containing the ß3 subunit, and which elicits at most a 20% potentiation of the GABA EClo response in transfected L (tk-) cells expressing the GABAA receptor containing the pz subunit.

In this aspect of the present invention, the potentiation of the GABA EC2o response in L (tk-) cells expressing either the ß3 or ß2 subunits of the human GABAA receptor can conveniently be measured by procedures readily available to the skilled worker, for instance, as described in C. Hill-Venning et al., Br. J. Pharmacol., 120,749-756 (1997); McKernan et al., Nat. Neurosci., 3, 587-592 (2000).

The compounds of use in this aspect of the invention will elicit at least a 30%, preferably at least a 40%, and more preferably at least a 50%, potentiation of the GABA EC2o response in transfected L (tk-) cells expressing the GABAA receptor containing the ß3 subunit. Moreover, the compounds of use in this aspect of the invention will elicit at most a 20%, preferably at most a 10%, and more preferably 0%, potentiation of the GABA EC2o response in transfected L (tk-) cells expressing the GABAA receptor containing the P2 subunit.

According to another aspect of the present invention, there is provided the use of GABAA receptor/33 functionally selective anesthetic agent for the manufacture of a medicament for providing anesthesia, wherein said anesthetic agent is an agonist at the GABAA receptor containing a p3 subunit, having potency (ECso) for such a receptor of 25 uM or less, when measured using a whole-cell clamp technique on rodent fibroblast L (tk) cells stably transfected with the GABAA receptor containing the ß3 subunit, and which has a potency (ECso) for the GABAA

receptor containing a pz subunit of at most 50 ut/I, when measured using a whole-cell clamp technique on rodent fibroblast L (tk) cells stably transfected with the GABAA receptor containing the p2 subunit.

This aspect of the present invention also provides a method for providing anesthesia comprising administering to a mammal a GABAA receptor pa functionally selective anesthetic agent in an amount equal to or in excess of an amount effective for anesthesia in order to induce or maintain anesthesia, wherein said anesthetic agent is an agonist at the GABAA receptor containing a (3s subunit, having potency (EC5o) for such a receptor of 25 uM or less, when measured using a whole-cell patch clamp technique on rodent fibroblast L (tk' cells stably transfected with the GABAA receptor containing the 03 subunit, and which has a potency (EC60) for the GABAA receptor containing a pz subunit of at most 50 uM, when measured using a whole-cell clamp technique on rodent fibroblast L (tk) cells stably transfected with the GABAA receptor containing the pz subunit.

In this aspect of the present invention, the GABA-mimetic action of the compounds in stably transfected rodent fibroblast L (tk) cells expressing either the p3 or pz subunits of the human GABAA receptor can conveniently be measured by procedures readily available to the skilled worker, for instance, as described in McKernan et al., Nat. Neurosci., 3, 587-592 (2000); D. Belelli et al., Proc. Natl. Acad. Sci., USA, 94,11031- 11036 (1997).

In order to elicit their behavioural effects, the compounds of use in the present invention will be brain-penetrant; in other words, these compounds will be capable of crossing the so-called"blood-brain barrier".

Preferably, the compounds of use in this aspect of the invention will be capable of exerting their beneficial therapeutic action following administration by the parenteral route.

For therapeutic application, pharmaceutical compositions may be provided which comprise one or more compounds of use in this invention

in association with a pharmaceutically acceptable carrier. Preferably these compositions adapted for parenteral administration.

In particular, pharmaceutical intravenous formulations of a compound of use in the present invention will comprise one or more pharmaceutically acceptable excipients. Suitable liquid inert excipients/carriers include Water for Injection (USP in the US) and saline solution.

Other suitable excipients and other accessory additives include solvents, for example, ethanol, glycerol and propylene glycol; stabilizers, for example, EDTA (ethyl diamine tetraacetic acid) and citric acid; antimicrobial preservatives, for example, benzyl alcohol, methyl paraben and propyl paraben; buffering agents, for example, citric acid/sodium citrate, potassium hydrogen tartrate, sodium hydrogen tartrate, acetic acid/sodium acetate, maleic acid/sodium maleate, sodium hydrogen phthalate, phosphoric acid/potassium dihydrogen phosphate and phosphoric acid/disodium hydrogen phosphate; and tonicity modifiers, for example, sodium chloride, mannitol and dextrose.

It will be appreciated that the compounds of the present invention may be administered either alone or, more commonly, in combination with one or more agents. Such agents include intravenous anesthetics and inhaled anesthetics, for example, halothane, enflurane, isoflurane, methoxyflurane and nitrous oxide. The requirements for general anesthesia and surgery may necessitate the administration of several intravenous agents with different actions to ensure hypnosis, analgesia, relaxation and control of visceral reflex responses. Suitable intravenous anesthetics include barbiturates, for example, thiopental, methohexital and thiamylal; benzodiazepines, for example, diazepam, lorazepam and midazolam; opioid analgesics, for example, morphine, meperidine, fentanyl, sufentanil and alfentanil; neuroleptic-opioid combinations, for example, droperidol combined with an opioid analgesic such as fentanyl; ketamine; and propofol.

The novel effect of a GABAA receptor 03 selective anesthetic agent according to the present invention may be demonstrated using a transgenic animal, especially a transgenic mouse. Studies using recombinant receptors expressed in cell lines, have shown that substituting asparagine (Asn) for serine (Ser) at position 289 of the human GABAA receptor pz subunit renders the resulting"mutant"receptors insensitive to etomidate. Furthermore, this point mutation does not affect the opening of the channel by GABA or the agonist actions of other classes of anesthetics such as barbiturates. The effect of GABAA receptor pg functionally selective anesthetic agents according to the present invention may be demonstrated using ß2N289S point mutation ("knock-in") mice.

Thus, a further aspect of the present invention is based on a new transgenic animal that contains a GABAA receptor 02 subunit gene in which a portion of the open reading frame which encodes a region determining the selectivity of etomidate on the 02 subunit polypeptide has been replaced with a corresponding portion of the GABAA receptor Pi subunit open reading frame. This animal is affected by anesthetic agents such as etomidate solely by virtue of the action of the drug on GABAA receptors containing the 03 subunit. The absence of the 02 subunit correlates with a dramatically improved recovery from the effects of an anesthetic agent such as etomidate. Thus, the transgenic animals of the invention offer a unique animal model in which the genetic and biochemical mechanisms responsible for slow recovery from the effects of anesthetic agents such as etomidate can be investigated.

Accordingly, the invention features a transgenic animal (e. g. , a non- human mammal, rodent, rat, mouse, rabbit, pig, cow, chicken, or fish) whose genomic DNA includes a gene having a GABAA receptor ß2 subunit promoter operably linked to a DNA sequence encoding a point mutated GABAA receptor ß2 subunit polypeptide. By"operably linked"is meant that a nucleotide sequence (e. g. , an open reading frame) is linked to a<BR> regulatory sequence (e. g. , a promoter) in a manner which allows for

expression of the nucleotide sequence in vitro or in vivo. The transgenic animal exhibits rapid recovery from the effects of an anesthetic agent such <BR> <BR> as etomidate, as compared to a reference animal (e. g. , the wildtype animal from which the transgenic animal was generated) whose genomic DNA does not contain the gene.

A"GABAA receptor pz subunit promoter"refers to a promoter that directs transcription of an RNA in a temporal and cell-type specific manner at least substantially similar, if not identical, to the expression of a wildtype GABAA receptor p2 subunit gene. Thus, a"GABAA receptor p2 subunit promoter"includes wildtype GABAA receptor p2 subunit promoters as well as such promoters containing insertions, deletions, or substitutions that do not affect the temporal and tissue-specific expression of the GABAA receptor p2 subunit promoter. Similarly, a"point mutated GABAA receptor (3 subunit polypeptide"is a polypeptide that has at least one biochemical or cellular activity associated with a wildtype GABAA receptor (3 subunit polypeptide, and at least one (and preferably one) point mutation in the region determining etomidate sensitivity e of the p2 subunit.

In addition, when the gene is made by replacing sequence encoding the region determining etomidate sensitivity, of a wildtype endogenous GABAA receptor p2 subunit gene with a corresponding GABAA receptor pi subunit open reading frame, the transgenic animal can be homozygous or heterozygous for the mutated GABAA receptor p2 subunit gene. The invention also features a method of producing a transgenic animal of the invention by replacing at least a portion of a GABAA receptor p2 subunit gene in the genomic DNA of the transgenic animal with a DNA insert comprising a first sequence encoding a point mutated GABAA receptor ß2 subunit polypeptide and a second sequence encoding a selectable marker; and removing the second sequence from the genomic DNA, thereby producing the transgenic animal.

In addition, the invention includes a nucleic acid comprising a GABAA receptor pz subunit promoter operably linked to a sequence

encoding a point mutated GABAA receptor pa subunit polypeptide, which is useful for producing a transgenic animal of the invention.

The transgenic animals of the invention can be used to elucidate the biochemical and genetic determinants that provide for the mechanism of action of a ß-2/3 selective anesthetic agent such as etomidate.

Suitable ß3 selective anesthetic agents for use in accordance with the present invention may be selected using methods that are readily apparent to a person of ordinary skill in the art. Thus, for instance, one such method for screening for ß3 selective anesthetic agents comprises : a) exposing cells expressing a functional GABAA receptor comprising the ps subunit (to the exclusion of any other ß subunit) to putative anesthetic agents which may potentiate the effects of GABA at said GABAA receptor; b) selecting those agents that potentiate the effect of GABA at the receptor in step (a); c) comparing the binding of the selected agents to the receptor in step (a) with the effects of the selected agents to other GABAA receptors; and d) determining those agents that potentiate the effect of GABA at the receptor in step (a) but with lower effects at other GABAA receptors.

It will be appreciated that of the other possible GABAA receptors referred to in steps (c) and (d), a functional GABAA receptor comprising the p2 subunit (to the exclusion of any other P subunit) is particularly preferred.

In addition, of the other possible GABAAreceptors referred to in steps (c) and (d), a functional GABAA receptor comprising the pi subunit (to the exclusion of any other ß subunit) is also preferred.

Stably co-transfected cell lines expressing suitable combinations of the GABAA subunits are well known in the art. Thus, for example, stably co-transfected eukaryotic cell lines capable of expressing human GABAA

receptors comprising at least one a, at least one 3 and at least one y subunit are described in International (PCT) Patent Publication Nos.

WO 92/22652 and WO 94/13799, the contents of which are incorporated herein by reference. Stably co-transfected eukaryotic cell lines capable of expressing suitable combinations of the GABAA subunits are also described in International (PCT) Patent Publication Nos. WO 96/10637, WO 98/23742 and WO 98/49293, the contents of which are incorporated herein by reference.

Other features or advantages of the present invention will be apparent from the following detailed description and also from the claims.

Introduction of a transgene into the fertilized egg of an animal (e. g., a mammal) is accomplished by any number of standard techniques in transgenic technology. See, for example, Hogan et al., Manipulating the Mouse Embryo : A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N. Y. , 1986. Most commonly, the transgene is introduced into the embryo by way of microinjection.

Once the transgene is introduced into the egg, the egg is incubated for a short period of time and is then transferred into a pseudopregnant animal of the same species from which the egg was obtained (Hogan et al., supra). In the case of mammals, typically 125 eggs are injected per experiment, approximately two-thirds of which will survive the procedure.

Twenty viable eggs are transferred into pseudopregnant mammal, four to ten of which will develop into live progeny. Typically, 10-30% of the progeny (in the case of mice) carry the transgene.

To identify the transgenic animals of the invention, progeny are examined for the presence of the transgene using standard procedures such as Southern blot hybridization or PCR. Expression of the transgene can also be assessed using Northern blots, Western blots, and immunological assays.

In an alternative method the transgene is introduced via the homologous recombination technique into the mouse genome as outlined in Example 1 in more detail. In brief, genomic DNA containing the gene of interest is isolated from a genomic DNA library, sub-cloned into plasmid vectors and a detailed restriction endonuclease map is established. A gene targeting vector containing a long and a short region of homology to the gene of interest, as well as a positive and a negative selection marker, is introduced into embryonic stem (ES) cells. The frequency of homologous recombination upon positive-negative selection is unpredictable, but ranges typically from 1-10%. Correctly targeted ES cell clones are injected into blastocysts and the resulting chimeras test bred for germ line transmission. Progeny are tested by Southern blot hybridization or PCR analysis for the correct integration of the transgene and a colony of homozygous and wild-type control animals is established.

The intravenous anesthetic etomidate is known to mediate its effects through GABAA receptors, and in particular by its action on pz-and 03-containing receptors. This selectivity is determined by a single amino acid, asparagine at position 289. In the/31 subunit, which has reduced sensitivity to etomidate, there is a serine instead of an asparagine. In a preferred aspect of the present invention, there is provided a transgenic animal with a point mutation (serine for asparagine) at position 289 of the Pz subunit. Thus, in particular, there is provided ß2N289$ point mutation (knock-in) mice.

Without further elaboration, it is believed that one skilled in the art can, based on the above disclosure and the description below, utilize the present invention to its fullest extent. The following examples are to be construed as merely illustrative of how one skilled in the art can practice the invention and are not limitative of the remainder of the disclosure in any way. All publications cited in this disclosure are hereby incorporated by reference.

EXAMPLE 1 Generation of GABAA receptor p2N289S knock-in mice The generation of mice carrying the codon change from Asn 289 to Ser 289 in the 32 subunit of the GABAA receptor in their genome was performed as follows. A murine 129/SvEv k fixII library (Stratagene, LaJolla, California) was screened using a PCR (polymerase chain reaction) product as a probe, which was amplified by using the following oligonucleotides: sense: 5'-TCT. CCA. TTC. TAG. ATT. ATA. AAC. TCA. TCA. CC-3' ; antisense: 5'-GGT. CCA. TCT. TGT. CGA. CAT. CCA. GGC-3'. Two positive hybridising X clones containing were subcloned via the NotI sites into pBluescript, one of them (A. 1.2) containing ca. 17.5 kb and the other (J. l. l) 11 kb of genomic DNA. These two clones were further subcloned, an extensive DNA restriction map was established and the full 17.5 kb long DNA sequence, which contained exons 6,7 and 8 of the ß2 subunit gene, was determined. A 2 kb ERV subclone of JPI-H containing exon 8 was used for the site-directed mutagenesis. Single stranded DNA was prepared and the Asp 289 to Ser 289 codon change labelled by the novel restriction endonuclease restriction site ScaI which was introduced by standard techniques for site directed mutagenesis using oligonucleotide 5'-ACC. ACA. ATC. AGT. ACT. CAC. CTC. CGG. G-3'. The 2 kb ERV clone containing the ß2N289S mutation was cloned into a 6 kb long SalI-ERV subclone (AE1-B) of full length clone A. 1.2. The 8 kb long SalI DNA fragment of this newly generated clone (clone 8) was introduced into the SalI site and the 2 kb ERV+ERI DNA fragment of subclone JH3.3 blunt ended into the HpaI site of the pBS246-neo-tk-1 multiple utility targeting vector. pBS246-neo-tk-1 consists of the loxP site containing plasmid pBS246 (Sauer, Methods in Enzymology, 225, 890 (1993) ) in which the phosphoglycerate kinase I (PGK) neo and the Herpes simplex virus thymidine kinase (TK) gene have been cloned. After linearization with NotI the targeting vector was introduced into AB2.2 ES cells (Lexicon Genetics Inc. ) as described, for instance, by Thomas and Capecchi, Cell,

51,503 (1987) and Rosahl et al., Cell, 75, 661 (1993). Homologous recombinants were identified by PCR using the oligonucleotides 5'-CTATGATGCCTCTGCTGCACGGGTTGC-3'and 5'-GGATGCGGTGGGCTCTATGGCTTCTGA-3'and were further confirmed by genomic Southern blotting. Presence of the (32N289S mutation was confirmed by cutting the 800 bp long PCR product amplified by primers 5'-TGGAAATTTGAGCAGCCCATTGTG-3'and 5'-AACCTTTCATCTTTAGTCCCCTGG-3'with the restriction endonuclease ScaI. Correctly targeted ES cell clones were injected into C57BL6 blastocysts and one clone gave rise to highly chimeric males, which transmitted the targeted allele into the germ line.

J32N289S +/- (heterozygous) mice were further bred with a cre-transgenic <BR> <BR> mouse (Schwenk et al., Nucleic Acid Res. , 23,5080 (1995) ) to remove the neomycin resistance gene in their offspring. cre-Mediated recombination was confirmed by PCR using oligonucleotides 5'-AGATCTAGGTGATGACTGTC-3'and 5'-AGACAGACGCCACATCACAC-3'. By further breeding, a colony of wildtype and homozygous P2N289S mice were generated and kept in a mixed 75% C57BL6/25% 129SvEv genetic background.

EXAMPLE 2 Anesthetic effects of etomidate in ß2N289S knock-in and wildtype mice Method Loss of righting reflex (LORR) was used as a crude behavioural measure of anesthesia. Mice (n=ll wildtype, n=10 knock-in for each dose) were dosed intraperitoneally (i. p. ) with 20,30 or 40 mg/kg etomidate. Each mouse was placed in a separate cage and tested for LORR by gently rolling the mouse on it back. When the righting reflex had disappeared (usually around 90 seconds) the mouse was laid on it back on a piece of corrugated plastic and the time until righting reflex was regained was recorded. In

order to avoid excessive heat loss during anesthesia a heat lamp was placed above the mice. To control for non-specific changes in anesthetic sensitivity induced by the mutation two different classes of anesthetics were also tested in this model: pentobarbitone, 50 mg/kg i. p. (n=ll) and midazolam, 80 mg/kg i. p. (n=5).

Results The duration of LORR in knock-in mice was nearly significantly similar to the duration seen in wildtype mice (Figure 2; Fl, s7=0. 005, p=0. 9498).

Likewise, there was no significant difference between knock-in and wildtype mice for pentobarbitone or midazolam treatment (Fl, 28=0. 61, p=0. 44). These data suggest that the anesthetic properties are mediated by the pg subunit and not by the (3 subunit.

EXAMPLE 3 Recovery of (32N289S and wildtype mice following etomidate anesthesia Method Wildtype (n=30) and knock-in (n=30) mice were trained to perform the rotarod test of motor co-ordination. Mice were placed on a rod revolving at 16 rpm and the time taken for them to fall off recorded. Each mouse was given successive training trials until it could remain on the rod for 3 consecutive trials of 120 second duration. Mice were then split into 3 groups (n=10 for each genotype) and dosed i. p. with 15,20 or 30 mg/kg etomidate. Mice were then placed back on the rotarod every 10 minutes (for 90 minutes), starting from the mean time for recovery of righting reflex for a given dose (obtained from experiment 1). In this way the rate of recovery of the ability to perform a motor task could be assessed.

Results Following 15 mg/kg etomidate both wildtype and knock-in mice recovered rotarod performance at a similar rate, such that both groups could stay on for approximately 120 seconds 90 minutes after testing began. However, as the dose of etomidate increased to 20 mg/kg and especially 30 mg/kg, wildtype mice recovered more slowly than knock-in mice. Figure 3 shows a clear effect on the rate of recovery with increasing dose of etomidate in the wildtype mice (Fis, 243 =5.80, p<0. 00005). In contrast, the knock-in mice show a similar recovery rate across the dose range tested (Fi8, 243=1. 45, p=0. 111). These data suggest that pz is the primary mediator of the ataxic effects of etomidate.

EXAMPLE 4 Level of anaesthesia as determined by electroencephalography is comparable in P2N289S mice and wild-type mice Method In order to compare the depth of anaesthesia in both genotypes, wildtype (n=4) and knock-in (n=4) mice were implanted with radiotelemetry transmitters for the measurement of electroencephalographic (EEG) data.

Mice were allowed to recover from the surgical procedure for 14-21 days before experiment. Mice were dosed with 12.5mg/kg etomidate by the intravenous route and EEG data was continuously sampled. Data sampled at 250Hz (transmitter bandwidth 0. 5-lOOHz, low pass data filter 70Hz). Data analysed using Somnologica 3 in lOOmSec epochs for % suppression activity per minute. Isoelectric window defined as waveform amplitude less than 5, uV (typical peak to peak amplitude-250uV).

Results Etomidate anaesthesia results in a characteristic EEG pattern which is called"burst suppression" (Vijn, P. C. M. & Sneyd, J. R., Br. J. Anaes., 81, <BR> <BR> 415 (1998) ). Such activity is characterised by large amplitude, fast spikes

interspersed by relatively isoelectric periods in the EEG. Such activity is evident in the EEG of both the wildtype and knock-in mice and quantification of such activity (% of each minute EEG in suppression) reveals no significant difference in the EEG pattern of both genotypes (FIGURE 4).

EXAMPLE 5 Increased hypnotic hangover in wildtype mice in comparison to knock-in mice following etomidate anaesthesia Method In order to quantify the amount of post-anaesthetic sleep, wildtype (n=4) and knock-in (n=4) mice were implanted with radiotelemetry transmitters for the measurement of electroencephalographic (EEG) data. Mice were allowed to recover from the surgical procedure for 13 days before experiment. Baseline EEG data was recorded on day 1 of the experimental protocol. On the 2nd experimental day, mice were dosed with 12.5mg/kg etomidate by the intravenous route and EEG data was continuously sampled. EEG data sampled at 250Hz (transmitter bandwidth 0. 5-lOOHz, low pass data filter 70Hz). Data analysed using Somnologica 3 in 8sec epochs and scored for either"wake"or"slow wave sleep"according to standard electrophysiological criteria (van Gool, W. A.

&Mirmiran, M. , Sleep, 9,335 (1986) ). Following recovery from etomidate anaesthesia, the amount of slow wave sleep was expressed as percentage of the circadian timed matched baseline.

Results Following etomidate anaesthesia (12.5mg/kg intravenous route) WT mice have an enhanced level of sleep in comparison to circadian matched baseline data up to 3 hours post recovery (ANOVA P<0.05) whilst the level of slow wave sleep in the knock-in mice following recovery is not statistically different (P>0.05). Wildtype mice therefore have an increased

"hypnotic hangover"following recovery from etomidate anaesthesia which may prolong the recovery phase (FIGURE 5).

Conclusions These data suggest that a (3s-selective agonist would still be an anesthetic and would have a reduced potential to impair function following a period of anesthesia.