Title:
SELF-ASSEMBLED NANOPARTICLE CONTAINING GB PROTEIN OF EB VIRUS, AND PREPARATION METHOD THEREFOR AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2022/120908
Kind Code:
A1
Abstract:
A self-assembled nanoparticle containing a gB protein of an EB virus, and a preparation method therefor and the use thereof. The self-assembled nanoparticle comprises a first polypeptide and a second polypeptide. The first polypeptide comprises a gB protein and a first carrier subunit, and the second polypeptide comprises a second carrier subunit. The first carrier subunit is I53-50A1, and the second carrier subunit is I53-50B.4PT1. In the self-assembled nanoparticle, the gB protein of the EB virus is first displayed on the surface of the nanoparticle. Compared with the antigen gB, the self-assembled nanoparticle has a larger particle size, a higher chemical stability and a higher binding capacity with the neutralizing antibody, which are beneficial for increasing the residence time thereof on the B cell antigen receptor and stimulating the generation of the antibody. At the same time, the self-assembled nanoparticle can induce a higher titer of an animal immune antibody, and can be used for preventing EB virus infections and treating diseases caused by EB virus infections.
Inventors:
ZENG MUSHENG (CN)
SUN CONG (CN)
ZENG YIXIN (CN)
FENG GUOKAI (CN)
KANG YINFENG (CN)
CHEN XINCHUN (CN)
ZHANG XIAO (CN)
ZHU QIANYING (CN)
LI JIANGPING (CN)
KONG XIANGWEI (CN)
SUN CONG (CN)
ZENG YIXIN (CN)
FENG GUOKAI (CN)
KANG YINFENG (CN)
CHEN XINCHUN (CN)
ZHANG XIAO (CN)
ZHU QIANYING (CN)
LI JIANGPING (CN)
KONG XIANGWEI (CN)
Application Number:
PCT/CN2020/136752
Publication Date:
June 16, 2022
Filing Date:
December 16, 2020
Export Citation:
Assignee:
UNIV SUN YAT SEN (CN)
SUN YAT SEN UNIV CANCER CENTER SYSUCC (CN)
SUN YAT SEN UNIV CANCER CENTER SYSUCC (CN)
International Classes:
C07K19/00; A61K9/14; A61K39/245; A61P31/22; A61P35/00; C12N15/62
Foreign References:
| CN110922488A | 2020-03-27 | |||
| CN111333733A | 2020-06-26 | |||
| EP2630967A1 | 2013-08-28 | |||
| US20050228074A1 | 2005-10-13 |
Other References:
BROUWER PHILIP J. M., ANTANASIJEVIC ALEKSANDAR, BERNDSEN ZACHARY, YASMEEN ANILA, FIALA BROOKE, BIJL TOM P. L., BONTJER ILJA, BALE : "Enhancing and shaping the immunogenicity of native-like HIV-1 envelope trimers with a two-component protein nanoparticle", NATURE COMMUNICATIONS, vol. 10, no. 1, 1 December 2019 (2019-12-01), pages 1 - 17, XP055941992, DOI: 10.1038/s41467-019-12080-1
BU WEI; JOYCE M. GORDON; NGUYEN HANH; BANH DALTON V.; AGUILAR FIONA; TARIQ ZESHAN; YAP MOH LAN; TSUJIMURA YUSUKE; GILLESPIE REBECC: "Immunization with Components of the Viral Fusion Apparatus Elicits Antibodies That Neutralize Epstein-Barr Virus in B Cells and Epithelial Cells", IMMUNITY, CELL PRESS, AMSTERDAM, NL, vol. 50, no. 5, 21 May 2015 (2015-05-21), AMSTERDAM, NL , pages 1305, XP085694424, ISSN: 1074-7613, DOI: 10.1016/j.immuni.2019.03.010
MARCANDALLI ET AL., CELL, vol. 177, 2019, pages 1420 - 1431
BALE ET AL., SCIENCE, vol. 353, 2016, pages 389 - 394
SNIJDER ET AL., IMMUNITY, vol. 48, 2018, pages 799 - 811
BU WEI; JOYCE M. GORDON; NGUYEN HANH; BANH DALTON V.; AGUILAR FIONA; TARIQ ZESHAN; YAP MOH LAN; TSUJIMURA YUSUKE; GILLESPIE REBECC: "Immunization with Components of the Viral Fusion Apparatus Elicits Antibodies That Neutralize Epstein-Barr Virus in B Cells and Epithelial Cells", IMMUNITY, CELL PRESS, AMSTERDAM, NL, vol. 50, no. 5, 21 May 2015 (2015-05-21), AMSTERDAM, NL , pages 1305, XP085694424, ISSN: 1074-7613, DOI: 10.1016/j.immuni.2019.03.010
MARCANDALLI ET AL., CELL, vol. 177, 2019, pages 1420 - 1431
BALE ET AL., SCIENCE, vol. 353, 2016, pages 389 - 394
SNIJDER ET AL., IMMUNITY, vol. 48, 2018, pages 799 - 811
Attorney, Agent or Firm:
JIAQUAN IP LAW (CN)
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