Title:
STABILIZING COMPOSITION FOR TETRAHYDROBIOPTERIN, AND METHOD FOR ANALYZING TETRAHYDROBIOPTERIN USING SAME
Document Type and Number:
WIPO Patent Application WO/2012/141553
Kind Code:
A3
Abstract:
The present invention relates to a stabilizing composition for tetrahydrobiopterin, comprising dithioerythritol and acid compounds having a pH of 1.5 to 6.5. The present invention also relates to a method for analyzing tetrahydrobiopterin using the stabilizing composition. According to the present invention, low-density tetrahydrobiopterin existing in a sample may be directly analyzed using a mass spectrometer, and such an analysis may be effectively used in the study of unknown physiological and pathological functions of tetrahydrobiopterin.
Inventors:
KIM HYUNG GUN (KR)
KIM HAK RIM (KR)
KIM HAK RIM (KR)
Application Number:
PCT/KR2012/002865
Publication Date:
March 07, 2013
Filing Date:
April 16, 2012
Export Citation:
Assignee:
UNIV DANKOOK IACF (KR)
KIM HYUNG GUN (KR)
KIM HAK RIM (KR)
KIM HYUNG GUN (KR)
KIM HAK RIM (KR)
International Classes:
A61K47/02; A61K31/4985; A61K31/519; A61P25/00; A61P25/16
Other References:
FIEGE, B. ET AL.: "Plasma tetrahydrobiopterin and its pharmacokinetic following oral administration.", MOLECULAR GENETICS AND METABOLISM., vol. 81, 2004, pages 45 - 51
HOWELLS,D.W. ET AL.: "Direct analysis oftetrahydrobiopterin in cerebrospinal fluid by high-performance liquid chromatography with redox electrochemistry: Prevention of autooxidation during strage and analysis.", CLINICA CHIMIA ACTA., vol. 167, no. 1, 1987, pages 23 - 30
ZHAO, Y. ET AL.: "Detection of tetrahydrobiopterin by LC-MS/MS in plasma from multiple species", BIOANALYSIS., vol. 1, no. 5, 2009, pages 895 - 903
CURTIUS,H. ET AL.: "Tetrahydrobiopterin biosynthesis, studies with specifically labeled (2H)NAD(P)H and 2H20 and of the enzymes involved.", EUROPEAN JOURNAL OF BIOCHEMISTRY., vol. 148, no. 3, 1985, pages 413 - 419
TOTH,M. ET AL.: "Chemical stabilization of tetrahydrobiopterin by L-ascorbic acid; contribution to placental endothelial nitric oxide synthase activity.", MOLECULAR HUMAN REPRODUCTION., vol. 8, 2002, pages 271 - 280
HOWELLS,D.W. ET AL.: "Direct analysis oftetrahydrobiopterin in cerebrospinal fluid by high-performance liquid chromatography with redox electrochemistry: Prevention of autooxidation during strage and analysis.", CLINICA CHIMIA ACTA., vol. 167, no. 1, 1987, pages 23 - 30
ZHAO, Y. ET AL.: "Detection of tetrahydrobiopterin by LC-MS/MS in plasma from multiple species", BIOANALYSIS., vol. 1, no. 5, 2009, pages 895 - 903
CURTIUS,H. ET AL.: "Tetrahydrobiopterin biosynthesis, studies with specifically labeled (2H)NAD(P)H and 2H20 and of the enzymes involved.", EUROPEAN JOURNAL OF BIOCHEMISTRY., vol. 148, no. 3, 1985, pages 413 - 419
TOTH,M. ET AL.: "Chemical stabilization of tetrahydrobiopterin by L-ascorbic acid; contribution to placental endothelial nitric oxide synthase activity.", MOLECULAR HUMAN REPRODUCTION., vol. 8, 2002, pages 271 - 280
Attorney, Agent or Firm:
SON, Min (6th Floor City Air Tower,36, Teheran-ro 87 gil, Samsung-don, Gangnam-gu Seoul 135-973, KR)
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Claims: