Title:
SUBSTANCE DETECTION METHOD USING AMINOACYLASE
Document Type and Number:
WIPO Patent Application WO/2023/002821
Kind Code:
A1
Abstract:
The present invention measures the glycine produced as a byproduct alongside the main product of a chemical reaction that uses hippuric acid and/or methyl hippuric acid as a starting material and is catalyzed by a hydrolytic enzyme and thereby detects the presence of at least the starting material. The hydrolytic enzyme is an aminoacylase that has the amino acid sequence of SEQ ID NO:1 or is a protein that has the amino acid sequence of SEQ ID NO:1 with one or more amino acid deletions, substitutions, or additions and can hydrolyze hippuric acid and methyl hippuric acid.
Inventors:
NISHIYA YOSHIAKI (JP)
TATSUMI KENTA (JP)
YAGURA KAZUKI (JP)
TATSUMI KENTA (JP)
YAGURA KAZUKI (JP)
Application Number:
PCT/JP2022/025936
Publication Date:
January 26, 2023
Filing Date:
June 29, 2022
Export Citation:
Assignee:
JOSHO GAKUEN EDUCATIONAL FOUND (JP)
NIPRO CORP (JP)
NIPRO CORP (JP)
International Classes:
C12N15/55; C12N9/80; C12Q1/34
Foreign References:
| JP2019187285A | 2019-10-31 | |||
| JP2013179925A | 2013-09-12 | |||
| JP2013179924A | 2013-09-12 | |||
| JP2019187285A | 2019-10-31 | |||
| JP5978658B2 | 2016-08-24 | |||
| JP5751109B2 | 2015-07-22 |
Other References:
GIRISH TAVAREKERE S., B VIVEK, COLACO MELWIN, MISQUITH SANDRA, GOPAL B.: "Structure of an amidohydrolase, SACOL0085, from methicillin-resistant Staphylococcus aureus COL", ACTA CRYSTALLOGRAPHICA SECTION F STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS, vol. 69, no. 2, 1 February 2013 (2013-02-01), pages 103 - 108, XP093027240, DOI: 10.1107/S1744309112049822
TAVAREKERE S. GIRISHVIVEK BMELWIN COLACOSANDRA MISQUITHB. GOPAL: "Structure of an amidohydrolase, SACOL0085, from methicillin-resistant Staphylococcus aureus COL", ACTA CRYSTALLOGRAPHICA SECTION F, vol. 69, 2013, pages 103 - 108, XP093027240, DOI: 10.1107/S1744309112049822
KYOTO ENCYCLOPEDIA OF GENES AND GENOMES, Retrieved from the Internet
NISHIYA, YYAMAMOTO, M.TAKEMOTO, J.KANO, S.NAKANO, S.: "Monomeric sarcosine oxidase exhibiting high substrate affinity and thermostability", INT. J. ANAL. BIO-SCI., vol. 4, 2016, pages 55 - 62
HIRUTA, M.NISHIYA, Y: "Creation of an L-mandelate oxidase via structure-guided design of engineered lactate oxidase", INT. J. ANAL. BIO-SCI., vol. 6, 2018, pages 25 - 29
SHIMOZAWA, YYOSHIDA, S.IKEDA, K.KATO, Y.TOYAMA, F.NISHIYA, Y: "Easy preparation of a stable membrane-bound lactate dehydrogenase for application on lactate biosensor", INT. J. ANAL. BIO-SCI., vol. 8, 2020, pages 65 - 70
IMAI SATSUKINAGOSHI KENTAROSHINKI SHOTABABA TOSHIAKINISHIYA YOSHIAKI: "The Society for Biotechnology", 2018, article "Functional Modification of Pyrococcus horikoshii-Derived Hippurate Hydrolase"
IMAI SATSUKINAGOSHI KENTAROSHINKI SHOTABABA TOSHIAKINISHIYA YOSHIAKI: "Development of Method and Enzyme for Enzymatic Assay of Hippuric Acid", THE SOCIETY OF ANALYTICAL BIO-SCIENCE, 2019
IMAI SATSUKINAGOSHI KENTAROSHINKI SHOTABABA TOSHIAKINISHIYA YOSHIAKI: "Functional Improvement of Hippurate Hydrolase", JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY AND AGROCHEMISTRY, 2019
IMAI SATSUKINAGOSHI KENTAROSHINKI SHOTABABA TOSHIAKINISHIYA YOSHIAKI: "Functional Improvement of Hippurate Hydrolase toward Practical Use", THE MOLECULAR BIOLOGY SOCIETY OF JAPAN, 2019
IMAI SATSUKINAGOSHI KENTAROSHINKI SHOTABABA TOSHIAKINISHIYA YOSHIAKI: "Development of Enzymatic Assay Method for Determining Total Exposure to Toluene and Xylene (1", THE SOCIETY OF ANALYTICAL BIO-SCIENCE, 2020
SUGINAKA MUNEYUKITORII GENTOIMAI SATSUKIBABA TOSHIAKINISHIYA YOSHIAKI: "Development of Enzymatic Assay Method for Determining Total Exposure to Toluene and Xylene (2", THE MOLECULAR BIOLOGY SOCIETY OF JAPAN, 2020
TAVAREKERE S. GIRISHVIVEK BMELWIN COLACOSANDRA MISQUITHB. GOPAL: "Structure of an amidohydrolase, SACOL0085, from methicillin-resistant Staphylococcus aureus COL", ACTA CRYSTALLOGRAPHICA SECTION F, vol. 69, 2013, pages 103 - 108, XP093027240, DOI: 10.1107/S1744309112049822
KYOTO ENCYCLOPEDIA OF GENES AND GENOMES, Retrieved from the Internet
NISHIYA, YYAMAMOTO, M.TAKEMOTO, J.KANO, S.NAKANO, S.: "Monomeric sarcosine oxidase exhibiting high substrate affinity and thermostability", INT. J. ANAL. BIO-SCI., vol. 4, 2016, pages 55 - 62
HIRUTA, M.NISHIYA, Y: "Creation of an L-mandelate oxidase via structure-guided design of engineered lactate oxidase", INT. J. ANAL. BIO-SCI., vol. 6, 2018, pages 25 - 29
SHIMOZAWA, YYOSHIDA, S.IKEDA, K.KATO, Y.TOYAMA, F.NISHIYA, Y: "Easy preparation of a stable membrane-bound lactate dehydrogenase for application on lactate biosensor", INT. J. ANAL. BIO-SCI., vol. 8, 2020, pages 65 - 70
IMAI SATSUKINAGOSHI KENTAROSHINKI SHOTABABA TOSHIAKINISHIYA YOSHIAKI: "The Society for Biotechnology", 2018, article "Functional Modification of Pyrococcus horikoshii-Derived Hippurate Hydrolase"
IMAI SATSUKINAGOSHI KENTAROSHINKI SHOTABABA TOSHIAKINISHIYA YOSHIAKI: "Development of Method and Enzyme for Enzymatic Assay of Hippuric Acid", THE SOCIETY OF ANALYTICAL BIO-SCIENCE, 2019
IMAI SATSUKINAGOSHI KENTAROSHINKI SHOTABABA TOSHIAKINISHIYA YOSHIAKI: "Functional Improvement of Hippurate Hydrolase", JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY AND AGROCHEMISTRY, 2019
IMAI SATSUKINAGOSHI KENTAROSHINKI SHOTABABA TOSHIAKINISHIYA YOSHIAKI: "Functional Improvement of Hippurate Hydrolase toward Practical Use", THE MOLECULAR BIOLOGY SOCIETY OF JAPAN, 2019
IMAI SATSUKINAGOSHI KENTAROSHINKI SHOTABABA TOSHIAKINISHIYA YOSHIAKI: "Development of Enzymatic Assay Method for Determining Total Exposure to Toluene and Xylene (1", THE SOCIETY OF ANALYTICAL BIO-SCIENCE, 2020
SUGINAKA MUNEYUKITORII GENTOIMAI SATSUKIBABA TOSHIAKINISHIYA YOSHIAKI: "Development of Enzymatic Assay Method for Determining Total Exposure to Toluene and Xylene (2", THE MOLECULAR BIOLOGY SOCIETY OF JAPAN, 2020
Attorney, Agent or Firm:
ARCO PATENT & TRADEMARK ATTORNEYS (JP)
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