Title:
SUBSTRATE ACTIVATION KIT AND METHOD OF DETECTING DNA OR THE LIKE BY USING THE SAME
Document Type and Number:
WIPO Patent Application WO/2002/012891
Kind Code:
A1
Abstract:
It is intended to provide a method whereby DNA can be conveniently and rigidly immobilized on a substrate at such a high density as achieved by the conventional methods and a method of accurately detecting DNA. A substrate activation kit comprising a phosphate buffer (pH 6), a solution A (an aqueous solution) of N-hydroxysuccinimide and a solution B (a dioxane solution) of 1-[3-(dimethylamino)propyl]3-ethylcarbodiimide; and a method of detecting DNA characterized by comprising spotting DNA on a substrate having been activated by using the above activation kit and then hybridizing the ] spotted DNA with fluorescence labeled DNA.
Inventors:
OKAMURA HIROSHI (JP)
TANGA MICHIFUMI (JP)
YAMAKAWA KAORU (JP)
OHBA MITSUYOSHI (JP)
TAKAGI KENICHI (JP)
TANGA MICHIFUMI (JP)
YAMAKAWA KAORU (JP)
OHBA MITSUYOSHI (JP)
TAKAGI KENICHI (JP)
Application Number:
PCT/JP2001/005246
Publication Date:
February 14, 2002
Filing Date:
June 20, 2001
Export Citation:
Assignee:
TOYO KOHAN CO LTD (JP)
OKAMURA HIROSHI (JP)
TANGA MICHIFUMI (JP)
YAMAKAWA KAORU (JP)
OHBA MITSUYOSHI (JP)
TAKAGI KENICHI (JP)
OKAMURA HIROSHI (JP)
TANGA MICHIFUMI (JP)
YAMAKAWA KAORU (JP)
OHBA MITSUYOSHI (JP)
TAKAGI KENICHI (JP)
International Classes:
C12Q1/68; G01N33/543; G01N33/552; (IPC1-7): G01N33/53
Domestic Patent References:
| WO1999066322A1 | 1999-12-23 |
Foreign References:
| JPH08500722A | 1996-01-30 | |||
| JP2001139532A | 2001-05-22 |
Other References:
SILVIA STORRI ET AL.: "A piezoelectric biosensor for DNA hybridisation detection", ANALYTICAL LETTERS, vol. 31, 1998, pages 1795 - 1808, XP002945037
MENGSU YANG ET AL.: "Covalent immobilization of oligonucleotides on modified glass/silicon surfaces for solid-phase DNA hybridization and amplification", CHEMISTRY LETTERS, 1998, pages 257 - 258, XP002945038
See also references of EP 1310793A4
MENGSU YANG ET AL.: "Covalent immobilization of oligonucleotides on modified glass/silicon surfaces for solid-phase DNA hybridization and amplification", CHEMISTRY LETTERS, 1998, pages 257 - 258, XP002945038
See also references of EP 1310793A4
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