SULFOXI INE AND SULFODIIMINE MATRIX METALLOPROTEINASE INHIBITORS

BACKGROUND OF THE INVENTION This invention relates to pharmaceutically active sulfoximine and sulfodiimine derivatized peptides useful as inhibitors of the matrix metalloproteinase (MMP) family of enzymes for use in modulating physiological functions or treating diseases and disease conditions associated with MMP modulation, for example: arthritic diseases, such as osteoarthritis (OA) , rheumatoid arthritis (RA) , septic arthritis, soft tissue rheumatism, polychondritis and tendonitis; tumor invasion in certain cancers; periodontal diseases; corneal ulceration, e.g., that induced by alkali or other burns, by radiation, by vitamin E or retinoid deficiency; glo erular diseases, such as proteinuria, dytrophobic epidermolysis bullosa; bone resorption diseases, such as osteoporosis, Paget's disease, hyperparathyroidism and cholesteatoma; birth control through preventing ovulation or implantation; angiogenesis relating to tumor growth or to the neovascularization associated with diabetic retinopathy and macular degeneration; coronary thrombosis associated with atherosclerotic plague rupture; and pulmonary emphysema. In addition to the compounds and their use, the invention also relates to their precursors, to their preparation and to pharmaceutical compositions using the compounds of the invention. The MMPs are a family of zinc-containing proteinases believed to be responsible for the metabolic turnover of protein components of the extracellular matrix of humans . At present there are at least eight known human MMP.

Various disease and disease conditions have been linked with the actions or presence of MMP, e.g., elevated levels of certain of these enzymes exists in

joints of arthritic humans and animals and therefore have been linked to the degradation of the major components of articular cartilage and basement membranes. It is presently believed that the collective action of the MMP on extracellular matrix macromolecules is responsible for the destruction of connective tissue, however, the precise role of each enzyme in the process is not yet well understood. It has also been reported that certain MMP may be instrumental in mediating certain normal physiological functions that involve the breakdown or development of tissue.

It has been desired to selectively inhibit certain MMP enzymes, specifically those which modulate certain diseases, physiological conditions and disease conditions, in order that such conditions could be controlled.

It has been surprisingly discovered that a family of sulfoximine and sulfodiimine derivatized polypeptides are potent inhibitors of MMP, thereby affording a method of treating MMP-mediated diseases and disease conditions, and controlling MMP-mediated physiological functions.

SUMMARY OF THE INVENTION

Novel sulfoximine and sulfodiimine derivatized peptides of the formula,

Formula I

wherein:

R ¹ is selected from the group consisting of lower- alkyl, hydroxy lower-alkyl, amino lower-alkyl, carbamoyl lower-alkyl, lower-alkyl carbonyl, lower-alkyoxyalkyl, aralkyl and heteroaralkyl;

X is NH or O; R ² is selected from the group consisting of hydrogen, lower-alkyl and aralkyl; R ³ is selected from the group consisting of hydrogen, lower-alkyl, amino lower-alkyl, guanyl lower- alkyl, aralkyl and heteroaralkyl; and R ⁴ is selected from the group consisting of lower alkyl, aralkyl and -CH(R ⁵ ) -C (0)NH ₂ , wherein R ⁵ is selected from the group consisting of hydrogen, lower-alkyl, amino lower-alkyl, guanyl lower-alkyl, imidazoylalkyl, hydroxymethyl, 1-hydroxy- ethyl, mercapto lower-alkyl, and methyl¬ thio lower-alkyl; or a pharmaceutically acceptable ester, ether or salt useful for modulating physiological functions or treating diseases and disease conditions associated with MMP modulation, e.g., arthritic diseases, such as osteoarthritis (OA) , rheumatoid arthritis (RA) , septic arthritis, soft tissue rheumatism, polychondritis and tendonitis; tumor invasion in certain cancers, periodontal diseases; corneal ulceration, e.g., that induced by alkali or other burns, by radiation, by vitamin E or retinoid deficiency; glomerular diseases, such as proteinuria, dytrophobic epidermolysis bullosa; bone resorption diseases, such as osteoporosis, Paget 's disease, hyperparathyroidism and cholesteatoma; birth control through preventing ovulation or implantation; angiogenesis relating to tumor growth or to the neovascularization associated with diabetic retinopathy and macular degeneration; coronary thrombosis associated

with atherosclerotic plaque rupture; and pulmonary emphysema.

DETAILED DESCRIPTION The term "alkyl" refers to a branched or straight chain monovalent saturated aliphatic hydrocarbon radical of one to twenty carbon atoms.

The term "lower-alkyl" refers to a branched or straight chain monovalent alkyl radical of one to six carbon atoms. This term is further exemplified by such radicals as methyl, ethyl, propyl, isopropyl, butyl (e.g., isobutyl, t-butyl, or -n-butyl), pentyl, and hexyl .

The term "aryl" refers to an aromatic monovalent carbocyclic radical, which can optionally be mono-, di-, tri- or tetra-substituted, independently, with lower alkyl (e.g., methylphenyl, ethylphenyl) , lower-alkyoxy (e.g., 4-methoxyphenyl) , hydroxy (e.g., 4- hydroxyphenyl) halo, carboxy, lower-alkoxycarbonyl, carbamoyl, mono- and dimethylcarbamoyl, lower-alkyl carbonyl (such as, methylcarbonyl and ethylcarbonyl) , hydroxymethyl, amino, trifluoromethyl, cyano or nitro.

The term "aralkyl" refers to the group -(lower alkyl) - (aryl) . For example, typical arylalkyl groups are e.g., phenylmethyl (i.e., benzyl) , phenyl- ethyl, 4-hydroxyphenylmethyl, or 4-methoxyphenylmethyl .

The term "heteroaryl" refers to aromatic monovalent carbocyclic radical having at least one heteroatom, i.e., nitrogen, oxygen or sulfur, which can optionally be mono- or di-substituted adjacent to the heteroatom, independently, with lower alkyl, halo, cyano, amino or trifluoromethyl . For example, typical hetero¬ aryl groups with one or more nitrogen atoms are tetrazoyl, pyridyl (e.g., 4-pyridyl, 3-pyridyl, 2-pyridyl) , indolyl, pyridazinyl, quinolinyl, 2-quinolinyl, 3-quinolinyl, imidazolyl, isoquinolinyl, pyrazolyl, pyrazinyl, pyrimidinyl, pyridonyl or

pyridazinonyl; typical oxygen heteroaryl radicals with oxygen atom are furanyl, or benzofuranyl; typical sulfur heteroaryl radicals are thienyl, and benzothiophenyl. The term "heteroaralkyl" refers to the group (heteroaryl) - (lower alkyl). For example, typical heteroaralkyl groups are e.g., imidazoyl lower-alkyl, such as, 4-imidazolylmethyl, 3-imidazolylmethyl, 4-imidazoylethyl, or indolyl lower-alkyl, such as, 2- indolylmethyl, 3-indolylmethyl, The term "guanyl" refers to the moiety carbamimidoylamino

The term "sulfoximine" refers to the moiety

The term "sulfodiimine" refers to the moiety

The term "blocking group" or "BG ⁿ " refer to a chemical group which exhibits the following characteristics. The group must react selectively in good yield to give a blocked or protected substrate that is stable to the projected reactions; and the blocking group must be selectively removable in good yield by readily available, preferably nontoxic reagents that do not attack the functional group(s) generated in such

projected reactions. For example, typical blocking groups are benzyloxycarbonyl, tert-butyldimethylsilyl, or benzyl [for additional blocking or protecting groups see " Protective Groups " , J.F.W. McO ie, Adv. Org. Chem . , 3, 191 (1963) or " Protective Groups in Organic Synthesis " , Theodora W. Greene and Peter G.M. Wilts, John Wiley & Sons, 2nd Edition, 1991] are used for protecting substrates containing the chemical moieties, such as 4- hydroxyphenylmethyl, 3-indolylmethyl, 4-aminobutyl, 3-guanylpropyl or 4-imidazoylmethyl .

The term "blocked" refers to a chemical moiety that has been treated with a blocking group.

The term "de-blocking" reagent refers to a reagent which is used to remove a blocking group, e.g., elemental Na and liquid ammonia for debenzylation of S- benzyl (Evans, D. A.; Mathre, D. J.; Scott, W. L. J. Org. Chem. 1985, 50, 1830-1835), 10% Pd/C with catalytic amount of eyelohexylamine and H ₂ gas for removing CBz from imine moiety, or tetrabutylammonium fluoride hydrate for remove t-butyldimethylsilyl moiety.

The term "chiral auxiliary" or "CA" refers to compounds that direct the synthesis of the desired amino acid or modified amino acid in a stereospecific manner. For example, ( 4S, 5R) -4-methyl-5-phenyl-2-oxazolidinone, or ( 4R, 5S) -4-methyl-5-phenyl-2-oxazolidinone.

"Enantiomers" are two stereoisomers whose molecules are non-superimposable mirror images of one another.

"Diastereoisomers" or "diastereomers" are stereoisomers with two or more centers of asymmetry and whose molecules are not mirror images of one another.

The term "racemic" means existing as a racemate, or as a 50-50 mixture of two enantiomers, also denoted by " dl " or "±". The terms "D" and/or " L" refers to the absolute configuration at an asymmetric carbon of a molecule

assigned according to experimental chemical correlation with that of the α-carbon of a modified or unmodified amino acid residue (using the absolute configuration of the α-carbon of D- or L-serine as the standard) . The designation " DL" indicates a mixture of the

D and L stereoisomers or that diastereomers were separated but not identified.

Isolation and purification of the compounds and intermediates described herein can be effected, if desired, by any suitable separation or purification procedure such as, for example, filtration, extraction, crystallization, column chromatography, preparative high pressure liquid chromatography (preparative HPLC) , thin-layer chromatography or thick-layer chromatography, or a combination of these procedures. Specific illustrations of suitable separation and isolation procedures can be had by reference to the examples hereinbelow. However, other equivalent separation or isolation procedures can also be used. The following convention of abbreviations and nomenclature has been adopted and will be used to name the compounds of the present invention.

Common naturally occurring amino acids relating to the present invention are listed below in tables according to their radical type (e.g., aliphatic, aromatic, basic or substituted aliphatic) .

Aliphatic Amino Radical Abbreviation Acids

Glycine H Gly

Alanine methyl Ala

Val 2-propyl Val

Leucine 2-methylpropyl Leu

Isoleucine 2-butyl He

Aromatic Amino Radical Abbreviation Acids

Phenylalanine benzyl Phe

Tyrosine 4- Tyr hydroxyphenylmethyl

Tryptophan 3-indolylmethyl Trp

Basic Amino Acids Radical Abbreviation

Lysine 4-aminobuty1 Lys

Arginine 3-guanylpropyl Arg

Histidine 4-imidazoylmethyl His

Substituted Amino Radical Abbreviation Acids

Cysteine thiolmethyl Cys

Methionine methylthioethyl Met

Serine hydroxymethyl Ser

Threonine 1-hydroxyethy1 Thr

The abbreviation "Ac-" refers to the acetyl radical.

The abbreviation "Bn-" or "Bnz-" refers to the benzyl radical.

The abbreviation "AcS-" refers to the thiol- acetyl radical.

The abbreviation "-OEt" refers the ethoxy radical .

The abbreviation "RS-" refers to a lower-alkyl sulfide radical.

The abbreviation "BnS-" refers to the benzylsulfi.de radical.

The abbreviation "TBS" refers to the tert- butyldimethylsilyl radical.

The abbreviation "Cbz" refers to the benzyl- oxycarbonyl radical, i.e.,

The abbreviation " (Phet) " refers to the modified amino acid with phenylethyl as the radical, i.e.,

The abbreviation " (Tyr-OCH ₃ ) " refers to the modified amino acid with 4-methoxyphenylmetbyl as the radical, i.e.,

Certain naming conventions are accepted to represent the structure of modified peptides. For example, a modification of the C=0 group of an amino acid residue is signifiec by the usual three-letter code for the residue followed by the formula of the group that has replaced the C=0, separated by a hyphen, all enclosed in parentheses, a modification of the NH group of a residue is analogously indicated by the three-letter code for the residue preceded by the formula of the group substituting for the NH, separated by a hyphen, all enclosed in parentheses, and the stereoconfiguration of the α-carbon of a residue is indicated by the letters L or D preceding the three-letter code for the residue. The absence of either letters, or the presence of both letters indicates a mixture of the L and D isomers, or that diastereomers were separated but not identified.

For example, for a dipeptide analogue of (L- Ala)-( -Leu)

in which the C=0 of Ala and NH of Leu are replaced by sulfoximine [S(O) (NH) ] and methylene moieties, respectively,

would be designated as ( -Ala-SO(NH) ) - (CH ₂ - -Leu)

For example, for a dipeptide analogue of (L- Ala)-( -Leu)

in which the C=0 of Ala and NH of Leu are replaced by sulfodimine [S(NH) ₂ ] and methylene moieties, respectively,

would be designated as (L-Ala-S(NH) ₂ ) - (CH ₂ -L-Leu)

Some representative compounds are named in the following examples.

For example,

where X is 0, R ¹ is CH ₃ , R ² is 2-methylpropyl, R ³ is phenylmethyl and R ⁴ is methyl, and the carbon that is the point of attachment for for R ² is in the L- configuration, is Me- (RS) -SO(NH) - (CH ₂ - -Leu)-Phe-Ala-NH ₂ .

For example,

where X is NH, R ¹ is methyl, R ² is 2-methylpropyl, R ³ is 3-indolylmethyl and R ⁴ is phenylmethyl, and the carbon that is the point of attachment for for R ² is in the L- configuration, is MeS(NH) ₂ - (CH ₂ - -Leu) -Trp-NHBn.

SYNTHESIS OF THE COMPOUNDS OF FORMULA I

As used in the Reaction Schemes, X, R ¹ , R ² , R ³ , R ⁴ and R ⁵ are the same as described in the Summary of the Invention.

Reactio-. Scheme A ill' itrates the stereo- specific preparation of novel s'l—foximine and sulfodi¬ imine compounds, i.e., the compounds of Formula I. The formulae depicted in Reaction Scheme A are indicated as a single group of isomers, i.e. L-isomer, however it should be noted that Reaction Scheme A can also be used for

preparing -isomer compounds by starting with D specific starting material.

Reaction Scheme B illustrates an alternate preparation of novel sulfoximine and sulfodiimine compounds, i.e., the compounds of Formula I. The formulae depicted in Reaction Scheme B are indicated as racemic, i.e., DL, however it should be noted that the stereoconfiguration of the compounds prepared by Reaction Scheme B is governed by the stereoconfiguration of the starting materials (e.g. L-specific starting material will result in L-specific product and -specific starting material will result in D-specific product) .

Reaction Scheme C illustrates an alternate stereospecific preparation of novel sulfoximine and sulfodiimine compounds, i.e., the compounds of Formula I, where a stereospecific intermediate of Reaction Scheme A (Formula 3, L- or D- ) is converted to an intermediate of Reaction Scheme B and converted to a L- or D- diastereo- eric compound of Formula I by following the procedures described in Reaction Scheme B.

REACTION SCHEME A STARTING MATERIALS

Chiral auxiliary compounds, e.g., ( 4S, 5-R) -4- methyl-5-phenyl-2-oxazolidinone, or ( 4R, 5S) -4-methyl-5- phenyl-2-oxazolidinone are commercially available from Aldrich Chemical Co.

PREPARATION OF FORMULA 2

C2) where X is halo

(1)

The enantiomeric specific preparation of compounds of Formula I begin with the stereospecific synthesis of the chiral center. A procedure for this type of synthesis using a chiral auxiliary (CA) is described in Evans, D. A.; Mathre, D. J.; Scott, W. L. J. Org. Chem. 1985, 50 , 1830-1835. For example, a chiral auxiliary, such as ( 4S, 5R) -4-methyl-5-phenyl-2- oxazolidinone is combined with an aprotic solvent, such as tetrahydrofuran, cooled to a temperature in the range of about -50°C to -100°C, preferably about -78°C with stirring. To this solution is added about 1 molar equivalent of a base, preferably, a lower-alkyl lithium base (e.g., -n-butyllithium) , and about 1-3 molar equivalents, preferably about 1 molar equivalent of a substituted acid halide (i.e., a compound of Formula 1, where R ² is hydrogen, methyl, 2-propyl, 2-methylpropyl, 2- butyl, phenylmethyl, blocked 4-hydroxyphenylmethyl, 3-indoylmethyl, 4-methoxyphenylmethy1 or phenethyl) . The reaction mixture is allowed to warm to a temperature in the range of about -10°C to 10°C, preferably about 0°C, and stirred for a period of about 15 to 45 minutes, preferably about 30 minutes. A salt solution, such as, Na ₂ C0 ₃ , or K ₂ C0 ₃ , preferably K ₂ C0 ₃ is added to the mixture and stirred at a temperature in the range of about 20- 30°C, preferably about room temperature for a period of about 30 minutes to 90 minutes, preferably about 60

minutes. The organic solvents are removed in vacuo, and the residue is extracted with an organic solvent (e.g., CH ₂ C1 ₂ ) . The extracts are combined, washed with water and brine, dried and evaporated yielding the desired optionally substituted carbonyl compound, i.e., the compound of Formula 2.

PREPARATION OF FORMULA 3

C3)

A compound of Formula 2 is dissolved in an aprotic solvent, e.g., tetrahydrofuran and combined with a lithium base solution (prepared, e.g., by combining about 1 molar equivalent of a di-substituted lower-alkyl amine with about 1 molar equivalent of a lithium base, preferably, -n-butyllithium in an aprotic solvent, e.g., tetrahydrofuran) . The mixture is stirred at a temperature in the range of about -50°C to -100°C, preferably about -78°C for a period of about 15 to 45 minutes, preferably about 30 minutes. To this solution is added about 1 molar equivalent of a benzyl halomethyl sulfide, preferably benzyl bromomethyl sulfide. The combined mixture is stirred for a period of about 1 to 3 hours, preferably 2 hours, at a temperature of about 0°C to -50°C, preferably about -25°C, and additional for a period of 1 to 3 hours, preferably about 2 hours, at a

temperature in the range of about -25°C to 25°C, preferably about 0°C. An aqueous acid, e.g., NH ₄ C1, is added to quench the reaction mixture. The organic solvents are removed in vacuo, and the resulting residue is extracted, washed, dried and evaporated to yield the desired enantiomerically pure optionally substituted benzyl thiol ether compound, i.e., the compound of Formula 3.

PREPARATION OF FORMULA 4

C }

A compound of Formula 3 is dissolved in an aprotic solvent, such as tetrahydrofuran. To this solution is added a solution of lithium benzyloxide prepared by combining about 2 molar equivalents of benzyl alcohol, about 1.5 molar equivalents of .n-butyllithium in an aprotic solvent, such as tetrahydrofuran over a period of about 15 to 45 minutes, preferably about 30 minutes, at a temperature in the range of about -20°C to 0°C, preferably about -10°C. The reaction mixture is allowed to warm to about 0°C and stirred for a period of about 1 to 2 hours, preferably about Vλ- hours. A weak aqueous acid, such as saturated NH ₄ C1 is used to quench the reaction. The organic solvents are removed by vacuum, and the residue is extracted, washed, dried and

evaporated. The residual material is further purified by chromatography, e.g., flash chromatography on silica gel yielding the enantiomerically pure optionally substituted benzyl ester benzylthioether, i.e., the compound of Formula 4.

PREPARATION OF FORMULA 5

C5

A compound of Formula 4 is combined with a solution of 30% anhydrous hydrogen bromide (about 4 molar equivalents) and glacial acetic (about 13 molar equivalents) . The mixture is stirred at a temperature in the range of about 25°C to 75°C, preferably about 50°C for a period of about 15 to 45 minutes, preferably about 30 minutes. The reaction mixture is cooled, diluted with water and extracted. The resultant extracts are washed several times to remove residual acid. The crude product is further purified by combining with KOH, extraction with ether, adjustment of pH, extraction with ether, drying and evaporation to give the desired 3-benzylthio- (L-2-optionally substituted)propanoic acid, i.e., the compound of Formula 5.

PREPARATION OF FORMULA 6

CBa) CBb) (6)

A compound of Formula 6 is formed by the following procedure, which is a modification of procedures reported in Bodanszky, M. ; Bodanszky, A. The Practi ce of Peptide Synthesis ; Springer-Verlag: New York, 1 ^~ 4; p 129-142. A compound of Formula 6a (where R ³ is iv .rogen, lower-alkyl, blocked 4-aminobutyl, blocked 3-guanylpropyl, blocked 4-imidazoylmethyl, phenylmethyl, blocked 4-hydroxyphenylmethyl, 3-indolylmethyl, 4- ethoxyphenylmethyl or phenethyl) is combined with about 1 to 1.2 molar equivalents of N-hydroxysuccinimide, 1 to

1.2 molar equivalents of 1, 3-dicyclohexylcarbodiimide and an anhydrous solvent (at about 1.2 mL/m olar equivalent) such as tetrahydrofuran at a temperature in the range of about 0°C to 10°C, preferably about 4°C under an inert atmosphere for a period of about 13 to 39 hours, preferably about 26 hours. The resulting precipitate is removed by filtration. To the filtrate is added about

1.3 molar equivalents of a compound of Formula 6b dissolved in THF or in water, and if R ⁴ is -CH(R ⁵ ) -C0 ₂ H, 1.3 molar equivalents of a strong base such as sodium hyc . xide in water is also added. The combined mixture is stirred at about room temperature for a period of about 12 to 24 hours, preferably about 18 hours. The solid residue is removed by filtration and the filtrate is diluted with saturated aqueous NaHC0 ₃ and is extracted with a non-polar solvent (e.g., CHC1 ₃ ) . If R ⁴ is lower-

alkyl or aralkyl, the organic layer is evaporated to give the crude protected amino acid amide. If R ⁴ is -CH(R ⁵ ) -C0 ₂ H, the aqueous layer is acidified and the resulting precipitate is collected, washed and dried to yield the crude protected dipeptide acid. The crude protected dipeptide acid is subjected again to the above procedure, using excess anhydrous ammonia in an anhydrous solvent such as tetrahydrofuran, to give the crude protected dipeptide amide.

The crude protected amino acid amide or crude protected dipeptide amide from this procedure is subjected to hydrogenolysis in ethanol over 10% Pd/C. The product is purified by flash chromatography (e.g., ethyl acetate-methanol, 10:1) to afford a compound of Formula 6.

PREPARATION OF FORMULA 7

C6) (73

A compound of Formula 7 is formed by the following procedure, which is a modification of a procedure reported in Bodanszky, M. ; Bodanszky, A. The Practice of Peptide Synthesis ; Springer-Verlag: New York, 1984; p 145.

A compound of Formula 5 is combined with about 1 molar equivalent of a single amino acid amide or dipeptide derivative [i.e., a compound of Formula 6 where R ³ is hydrogen, methyl, 2-propyl, 2-methylpropyl, 2-butyl, blocked 4-aminobutyl, blocked 3-guanylpropyl, blocked 4-

imidazoylmethyl, phenylmethyl, blocked 4-hydroxyphenyl- methyl, 3-indolylmethyl, 4-methoxyphenylmethyl or phenethyl) and R ⁴ is lower-alkyl, aralkyl or -CH(R ⁵ )- C(0)NH ₂ where R ⁵ is hydrogen, lower-alkyl, amino lower- alkyl, guanyl lower-alkyl, imidazoylalkyl, hydroxymethyl, 1-hydroxyethyl, mercapto lower-alkyl, or methylthio lower-alkyl] 1-hydroxybenzotriazole (about 1-1.2 molar equivalents) and triethylamine in anhydrous 1,2- dimethoxyethane at about 0°C. To this solution is added dicyclohexylcarbodiimide (about 1-1.2 mmol) . The reaction mixture is stirred at a temperature in the range of about 15-30°C, preferably about room temperature for a period of about 12-24 h, preferably about 18 h. The reaction is worked up by removing the solvent, adding a nonpolar organic solvent, e.g., CH ₂ C1 ₂ , and filtering the mixture. The filtrate is evaporated and the residue purified by chromatography, e.g., flash chromatography on silica gel, to yield the desired benzylsulfide derivatized modified polypeptide, i.e., the compound of Formula 7.

PREPARATION OF FORMULA 8

CB

A compound of Formula 7 is debenzylated following a procedure using sodium in liquid ammonia (described in Evans, D. A.; Mathre, D. J.; Scott, W. L. J. Org. Chem. 1985, 50, 1830-1835) . To a solution of the benzyl thioether in a nonpolar solvent such as, diethyl

ether, THF, preferably THF and liquid NH ₃ , about 2-5 molar equivalents, preferably about 3.5 molar equivalents of elemental Na is added over a period of about 15 to 45 minutes, preferably about 30 minutes. The mixture is stirred for about an additional 10 min, solid NH ₄ C1 is added and the NH ₃ is allowed to evaporate. The reaction mixture is partitioned and the aqueous layer is extracted. The combined organic layers are washed, dried, and evaporated. The residue is purified by chromatography, e.g., flash chromatography on silica gel, to yield the desired mercaptan derivatized polypeptide, i.e., the compound of Formula 8.

PREPARATION OF FORMULA 10

(10]

A compound of Formula 8 is added to a solution of elemental Na (about 1.1 molar equivalents) and methanol . To this solution is added about 1.5 molar equivalents of a lower-alkyl halide (i.e., a compound of Formula 9 where X is halo) . The mixture is stirred for a period of about 4-8 hours, preferably about 6 hours at a temperature in the range of about 40°C. At the completion of the reaction, the solvent is removed, the residue partitioned and the aqueous layers extracted. The combined organic layers are dried and evaporated. The resultant residue is purified by chromatography, e.g., flash chromatography on silica gel, yielding the

desired lower-alkyl sulfide polypeptide, i.e., the compound of Formula 10.

PREPARATION OF FORMULA 11

(11)

A compound of Formula 10 is dissolved in solvent, such as ethyl acetate, methylene chloride, methanol or combination of methylene chloride and methanol (preferably a combination of methylene chloride and methanol at about a 2:1 ratio) . The solution is cooled to a temperature in the range of about 0°C to -20°C, preferably about -10°C. To this solution is added about 1 molar equivalent of a strong oxidizing reagent, such as a peracid, sodium metaperiodate in methanol- water, t-butyl hypochlorite in methanol, hydrogen peroxide in water, acetone or acetic acid, or m-chloro¬ peroxybenzoic acid preferably m-chloroperoxybenzoic acid. The reaction mixture is stirred for a period of about 4 to 12 hours, preferably about 8 hours. At completion of the reaction, the solvents are removed, the residue triturated, and the resultant residue purified by chromatography, e.g., flash chromatography on silica gel, to yield the desire sulfoxide derivatized polypeptide as a mixture of two diastereomers, i.e., the compound of Formula 11. The mixture is taken to the next step without further separating the two diastereomers .

PREPARATION OF FORMULA I

Formula I

A compound of Formula 11 (as a mixture of two diastereomers) is dissolved in a solvent, such as THF. To this solution is added about 3 molar equivalents of an amination reagent, such as O-mesitylsulfonylhydroxyl- amine . The reaction mixture is stirred at a temperature in the range of 20°C to 30°C, preferably about 25°C, for a period of about 8 to 12 hours, preferably about 10 hours. At the completion of the reaction, the mixture is partitioned, and the pH is adjusted to about pH 9. The aqueous layer is extracted, the combined organic layers dried and then evaporated. The resultant residue is purified by chromatography, e.g., flash chromatography followed by preparative TLC on silica gel, to yield the desired sulfoximine compound as a mixture of two diastereomers, i.e., the compound of Formula I (where X and Y are 0 and NH) .

REACTION SCHEME B STARTING MATERIALS The compounds of di-lower alkyl malonate and

Formula 12A (where R ² is methyl, 2-propyl, 2-methylpropyl, 2-butyl, phenylmethyl, blocked 4-hydroxyphenylmethyl, blocked 3-indoylmethyl, 4-methoxyphenylmethyl or phenethyl) are commercially available from the Aldrich Chemical Company, or can be prepared without undue experimentation by those of ordinary skill in the art.

PREPARATION OF FORMULA 12

lower-a O-lower-alkyl di lo wer-alkyl malonate (12A) (12)

A solution of sodium ethoxide is formed by dissolving about 1 molar equivalent of sodium in absolute ethanol (about 500 mL/mole) . To this solution is added about 1 molar equivalent of di lower-alkyl malonate, such as dimethylmalonate or diethyl alonate, preferably diethylmalonate with cooling under an inert atmosphere. To the resulting solution is added about 1 molar equivalent of a compound of Formula 12A where R ² is lower- alkyl, aralkyl or heteroaralkyl and X is halo. The reaction mixture is refluxed under an inert atmosphere for a period of 7 to 21 hours, preferably about 14 hours. The ethanol is removed and the residue is partitioned between a non-polar organic solvent and water. The aqueous layer is extracted. The combined organic layers are combined, dried over a drying agent (e.g., Na ₂ S0 ₄ ) and evaporated. The residue is distilled to give a diethyl optionally substituted malonate.

The diethyl optionally substituted malonate is combined with 95% ethanol (about 1 mL/mmole) . To this solution is added about 4 molar equivalent of KOH in 95% ethanol (about 12 mL/g) . The reaction mixture is stirred at about room temperature (i.e., about 25°C) for a period of about 8 to 24 hours, preferably about 16 hours, and then refluxed for a period of about 30 to 90 minutes, preferably about 1 hour. The mixture is cooled, diluted

and extracted with a non-polar organic solvent. The aqueous layer is cooled to about 0°C, acidified to a pH<l and extracted with a non-polar organic solvent. If neccessary, the aqueous layer is further continuously extracted for a period of about 18 hours. The organic layers are combined, dried over a drying agent (e.g., Na ₂ S0 ₄ ) and evaporated to afford the desired optionally substituted malonic acid, i.e., a compound of Formula 12.

PREPARATION OF FORMULA 13

(12) (13)

An optionally substituted malonic acid, i.e. , a compound of Formula 12 is dissolved in enough 37% aqueous formalin to provide about 5 molar equivalents of formaldehyde. To this solution is added about 1 molar equivalent of a secondary amine base, such as diethyl- amine. The reaction mixture ^' is stirred at a temperature in the range of about 20°C to 30°C, preferably about room temperature, for a period of about 1 to 4 hours, preferably about 3 hours. The reaction mixture is then refluxed for a period of about 1 to 3 hours, preferably about 2 hours . The mixture is allowed to cool to about room temperature and diluted with a solvent such as methylene chloride, and extracted with a base, such as NaHC0 ₃ . The aqueous layer is acidified and extracted. The organic layer is dried, (over a drying agent) and evaporated to yield the desired 2-optionally substituted 2-propenoic acid, i.e., the compound of Formula 13.

PREPARATION OF FORMULA 15

(15)

A compound of Formula 13 is combined with about 2 molar equivalents of thiolacetic acid (i.e., a compound of Formula 14) and stirred under an inert atmosphere for a period of about 20 to 32 hours, preferably about 26 hours. The excess thiolacetic acid is removed yielding the desired 2-optionally substituted 3-acetylthiopro- panoic acid, i.e., the compound of Formula 15.

PREPARATION OF FORMULA 16

(16)

A compound of Formula 15 is combined with a strong base, such as concentrated NH ₄ OH, and stirred at a temperature in the range of about 20°C to 30°C, preferably about room temperature for a period of about 30 to 90 minutes, preferably about 60 minutes. At the completion of the reaction, the mixture is acidified to a pH of about 4 to 5. The mixture is extracted with an organic solvent, such as, methylene chloride, and the organic layers are combined and evaporated yielding the desired 2-optionally substituted 3-mercapto propanoic acid, i.e., the compound of Formula 16.

PREPARATION OF FORMULA 17

A compound of Formula 16 is dissolved in a solvent, such as methanol with about 2 molar equivalents of a base such as sodium methoxide. To the solution is added about 1.2 molar equivalents of a lower-alkyl or aralkyl halide (i.e., a compound of Formula 9 where X is halo) . The solution is stirred for a period of about 4 to 12 hours, preferably about 8 hours at a temperature in the range of about 20°C to 30°C, preferably about room temperature. Water is added to the solution followed by acidification and extraction. The organic layer is dried and evaporated yielding the desired lower-alkyl or aralkyl sulfide modified carboxylic acid, i.e., the compound of Formula 17.

PREPARATION OF FORMULA 18

The compound of Formula 17 is dissolved in an aprotic solvent, such as tetrahydrofuran. A reducing reagent, such as 1 M BH ₃ in THF is added to the solution at about 0°C in a gradual manner with stirring over a period of about 15 minutes. The solution is allowed to

warm to about room temperature and stirred for a period of about 2 to 4 hours, preferably about 3 hours. The reaction mixture is cooled to a temperature in the range of 0°C and quenched by the gradual addition of water. The mixture is partitioned, and the organic layer is washed and dried (over a drying agent) . The solvent is removed and the residue is purified by chromatography, e.g., flash chromatography, to give the desired lower- alkyl sulfide 2-optionally substituted propanol, i.e., the compound of Formula 18.

PREPARATION OF FORMULA 19

(19)

A compound of Formula 18 is dissolved in a solvent, such as DMF. To the solution is added about 2.3 molar equivalents of a base, such as imidazole and 1 molar equivalent of a blocking agent, such as, t-butyl- dimethylsilyl chloride. The reaction mixture is stirred at a temperature in the range of about 20°C to 30°C, preferably about room temperature, for a period of about 5 to 9 hours, preferably about 7 hours. At the completion of the reaction, the mixture is partitioned and the organic layers are washed, dried and evaporated. The resultant residue is purified by chromatography, e.g., flash chromatography on silica gel, yielding the desired lower-alkyl sulfide 2-optionally substituted blocked propanol, i.e., the compound of Formula 19.

PREPARATION OF FORMULA 20

(2D)

A diimine moiety is introduced into a compound of Formula 19 using a modification of the procedure described in Mock, W. L.; Tsay, J. T. J. Am . Chem . Soc . 1989, 111 , 4461 -4472 . A solution is formed with a compound of Formula 19 in a solvent, such as tetrahydro¬ furan, diethyl ether, or acetonitrile, preferably acetonitrile, and about an equal volume of anhydrous liquid ammonia under an inert atmosphere at a temperature in the range of about -40°C to -70°C, preferably about -55°C. To the solution is added about 2.5 molar equivalents of N-chlorosuccinimide or t-butylhypo- chlorite, preferably N-chlorosuccinimide in a solvent such as acetonitrile in a gradual manner. The reaction mixture is stirred for a period of about 15 to 45 minutes, preferably about 30 minutes, at a temperature in the range of about -40°C to -70°C, preferably about -55°C. The mixture is allowed to warm to about room temperature and stirred for a period of about 12 to 24 hours, preferably about 18 hours. The solvent (s) is removed and the residue is partitioned. The organic layer is dried, and evaporated. The residue is purified by chromatography, e.g., flash chromatography, to yield the desired sulfodiimine modified derivative, i.e., the compound of Formula 20.

PREPARATION OF FORMULA 21

D L

(20) * ^R \ _C/ ^{C H} - C H C H ₂ - 0(BG ¹ ) n (B G ) N ^X N[B G ² )

( 21)

To a solution of the sulfodiimine (a compound of Formula 20) in a solvent, such as methylene chloride, diethyl ether or THF, preferably methylene chloride, is added about 5 molar equivalents of a base, such as pyridine, or triethylamine, preferably pyridine and about 4 molar equivalents of benzyl chloroformate. The mixture is stirred for a period of about 4 to 8 hours, preferably about 6 hours at a temperature in the range of about 20 to 30°C, preferably about room temperature. A base, such as NaHC0 ₃ , is added to the mixture. The mixture is then extracted, the organic layer isolated and evaporated, and the residue chromatographed, e.g., flash chromatography on silica gel to yield the desired blocked diimine compound, i.e., the compound of Formula 21.

PREPARATION OF FORMULA 22

DL

(22)

To a solution of the compound of Formula 21 in a solvent, such as diethyl ether, THF, methylene chloride, preferably THF, is added about 2 molar equivalents of a deblocking reagent, such as tetrabutyl-

ammonium fluoride hydrate. The reaction mixture is stirred for a period of about 1 to 4 hours, preferably about 2.5 hours, at a temperature in the range of 20 to 30°C, preferably about room temperature. At completion of the reaction, the mixture is diluted with an organic solvent, such as ethyl acetate and washed. The organic layer is dried, and evaporated. The residue is further purified by chromatography, e.g., flash chromatography on silica gel to yield the desired blocked diimine compound, i.e., Formula 22.

PREPARATION OF FORMULA 23

0

DL

(22) OH

(23)

To a solution of the compound of Formula 22 (dissolved in a polar solvent, such as acetone, or methanol, preferably acetone) is added an oxidizing reagent, such as Jones' reagent, (i.e., an aqueous chromic acid solution) in a dropwise manner at a temperature in the range of about 0°C with stirring. After completion of the addition, the mixture is stirred for a period of about 2 to 4 hours, preferably about 3 hours. The reaction mixture is poured into water and extracted. The organic layer is collected, dried and evaporated. The residue is purified by chromatography, e.g., flash chromatography on silica gel to give the desired blocked diimine modified amino acid, i.e., the compound of Formula 23.

PREPARATION OF FORMULA 24

0 (B) (24)

A compound of Formula 23 is coupled with a single amino acid amide, or a dipeptide derivative [i.e., a compound of Formula 6 where R ³ is hydrogen, methyl, 2- 5 propyl, 2-methylpropyl, 2-butyl, blocked 4-aminobutyl, blocked 3-guanylpropyl, blocked 4-imidazoylmethyl, phenylmethyl, blocked 4-hydroxyphenylmethyl, 3-indolyl- ethyl, 4-methoxyphenylmethyl or phenethyl) and R ⁴ is lower-alkyl, aralkyl or -CH(R ⁵ ) -C(O)NH ₂ where R ⁵ is 0 hydrogen, lower-alkyl, amino lower-alkyl, guanyl lower- alkyl, imidazoylalkyl, hydroxymethyl, 1-hydroxyethyl, mercapto lower-alkyl, or methylthio lower-alkyl] following the procedures described previously in Reaction Scheme A (Preparation of Formula 7) . 5

PREPARATION OF FORMULA I

Formula I

A compound of Formula 24 is dissolved in a solvent, such as ethanol, or methanol, preferably ethanol. To the solution is added a hydrogenation catalyst, such as 10% Pd/C (palladium on carbon) , a catalytic amount of an amine base (such as, cyclohexyl- a ine) . H ₂ gas is bubbled through the solution for a period of about 2 to 4 hours, preferably about 3 hours. The mixture is filtered and the solids are washed. The filtrates are combined, evaporated and purified by chromatography, e.g., flash chromatography on silica gel to yield the desire diimine modified polypeptide compound, i.e., the compound of Formula I.

REACTION SCHEME C PREPARATION OF FORMULA 25

O) (25)

A compound of Formula 25 is prepared following the procedures described in Hollady, M. W. ; Salituro, F. G.; Rich, D.H., J. Med Chem . 1987, 30 , 374-383) . About . ' molar equivalent of CaCl ₂ and about 3.5 mL/mmolar equivalents of an alcoholic solvent, such as absolute EtOH are combined. The solution is stirred until the solid is dissolved and the solution is cooled to a temperature in the range of about -10°C to 10°C, preferably about 0°C. To the solution is added about 2 molar equivalents of a reducing agent, preferably NaBH ₄ ,

and the solution is stirred for about 0.5 h. To the solution, is added a compound of Formula 3 (prepared as described in Reaction A) in a solvent, such as THF or diethyl ether, preferably THF (about 1.0 mL/mmolar equivalent) in a gradual manner. Upon completion of the addition, the reaction mixture is stirred at a temperature in the range of about -10°C to 10°C, preferably about 0°C under an inert atmosphere for a period of about 2 to 6 hours, preferably about 4 hours. The reaction mixture is quenched with an organic solvent (e.g., EtOAc) , followed by aqueous solutions (e.g., water followed by acetic acid) and acidified to a pH of about 2. The aqueous layer is extracted with an organic solvent (e.g., EtOAC) , the organic layers are combined, washed, dried and evaporated yielding the desired enantiomerically pure optionally substituted benzylthioether alcohol, i.e., the compound of Formula 25.

PREPARATION OF FORMULA 26

(26)

A compound of Formula 25 is dissolved in a solvent, such as DMF. To the solution is added about 2.3 molar equivalents of a base, such as imidazole and

1 molar equivalent of a blocking agent, such as, t-butyl- dimethylsilyl chloride. The reaction mixture is stirred at a temperature in the range of about 20°C to 30°C, preferably about room temperature, for a period of about 5 to 9 hours, preferably about 7 hours. At the completion of the reaction, the mixture is partitioned

and the organic layers are washed, dried and evaporated. The resultant residue is purified by chromatography, e.g., flash chromatography on silica gel, yielding the desired benzylthioether 2-optionally substituted blocked propanol, i.e., the compound of Formula 26.

PREPARATION OF FORMULA 27

(27)

A compound of Formula 26 is debenzylated following a procedure using sodium in liquid ammonia

(described in Evans, D. A.; Mathre, D. J.; Scott, W. L. J. Org. Chem. 1985, 50, 1830-1835) . To a solution of the benzylthioether in a nonpolar solvent such as, diethyl ether, THF, preferably THF and liquid NH ₃ , about 2-5 molar equivalents, preferably about 3.5 molar equivalents of elemental Na is added over a period of about 15 to 45 minutes, preferably about 30 minutes. The mixture is stirred for about an additional 10 min, solid NH ₄ C1 is added and the NH ₃ is allowed to evaporate. The reaction mixture is partitioned and the aqueous layer is extracted. The combined organic layers are washed, dried, and evaporated. The residue is purified by chromatography, e.g., flash chromatography on silica gel, to yield the desired mercaptan blocked alcohol, i.e., the compound of Formula 27.

PREPARATION OF FORMULA 28

(9)

(2B)

About 100 molar equivalents of NaOMe (e.g., 25 mL of 0.106 M NaOMe) in a solvent, such as MeOH, is added in a gradual manner to a compound of Formula 27 in a solvent, such as MeOH (about 4 mL/mmolar equivalents) . To this solution is added about 1.1 molar equivalents of a lower-alkyl halide (i.e., a compound of Formula 9 where X is halo) . The reaction mixture is allowed to stand for a period of about 12 to 24 hours, preferably about 18 hours at a temperature in the range of about 20°C to 30°C, preferably about room temperature. The mixture is diluted and acidified. The aqueous layer is extracted with an organic solvent (e.g., CH ₂ C1 ₂ ) . The organic extract is dried, evaporated and purified by chroma¬ tography (e.g., flash chromatography on silica gel) yielding the desired lower-alkyl sulfide 2-optionally substituted blocked propanol, i.e., the compound of Formula 28.

PREPARATION OF FORMULA 29

(29)

By following the procedures described in

Reaction Scheme B, Preparation of Formula 20 (e.g.,

Example 17) the desired enantiomerically pure sulfodi¬ imine modified derivative, i.e., the compound of Formula 29 is obtained.

PREPARATION OF FORMULA 30

(3D)

By following the procedures described in Reaction Scheme B, Preparation of Formula 21 (e.g., Example 18) the desired enantiomerically pure blocked diimine compound, i.e., the compound of Formula 30 is obtained.

PREPARATION OF FORMULA 31

(31)

By following the procedures described in Reaction Scheme B, Preparation of Formula 22 (e.g., Example 19) the desired enantiomerically pure blocked diimine compound i.e., the compound of Formula 31 is obtained.

PREPARATION OF FORMULA 32

(32)

0 By following the procedures described in Reaction Scheme B, Preparation of Formula 23 (e.g., Example 20) the desired enantiomerically pure blocked diimine modified amino acid, i.e., the compound of Formula 32 is obtained. 5

PREPARATION OF FORMULA 33

(B) (33) 5

By following the procedures described in Reaction Scheme B, Preparation of Formula 24 (e.g, Example 21) the desired enantiomerically pure blocked diimine modified polypeptide compound, i.e., the compound 0 of Formula 33 is obtained.

PREPARATION OF FORMULA I

Form ula I

By following the procedures described in

Reaction Scheme B, Preparation of Formula I (e.g., Example 22), the desired enantiomerically pure diimine modified polypeptide compound, i.e., the enantiomerically pure compound of Formula I is obtained.

PREFERRED COMPOUNDS Presently preferred is the compound of Formula I where R ¹ is lower-alkyl .

Especially preferred is the compound of Formula I where R ¹ is methyl.

Also especially preferred is the compound of Formula I where R ¹ is -n-butyl .

Of the compound where R ¹ is methyl, most preferred is the compound of Formula I where R ² is 2- methylpropyl, particularly where the carbon that is the point of attachment for R ² is in the L-configuration.

Of the compound where R ¹ is n-butyl, most preferred is the compound of Formula I where R ² is 2- methylpropyl, particularly where the carbon that is the point of attachment for R ² is in the L-configuration. Of the compound where R ¹ is n-butyl, most preferred is the compound of Formula I where R ² is 4- methoxyphenylmethyl, particularly where the carbon that is the point of attachment for R ² is in the L- configuration.

UTILITY, TESTING AND ADMINISTRATION GENERAL UTILITY

The compounds of this invention, including the pharmaceutically acceptable esters, ethers, or salts thereof, and the compositions containing them are useful for modulating physiological functions or treating diseases and disease conditions associated with the modulation of MMP activity, e.g., arthritic diseases, such as osteoarthritis (OA) , rheumatoid arthritis (RA) , septic arthritis, soft tissue rheumatism, polychondritis and tendonitis; tumor invasion in certain cancers, periodontal diseases; corneal ulceration, e.g., that induced by alkali or other burns, by radiation, by vitamin E or retinoid deficiency; glomerular diseases, such as proteinuria, dy ^~ rophobic epidermolysis bullosa; bone resorption diseases, such as osteoporosis, Paget's disease, hyperparathyroidism and cholesteatoma; birth control through preventing ovulation or implantation; angiogenesis relating to tumor growth on to the neovascularization associated with diabetic retinopathy and macular dege ^* "~ati- : coronary thrombosis associated with osclei c p-Lc-que rupture; and pulmonary emphy. le cr >unds of this invention inhibit MMP, tthheerreebb'' //iiddii means of controlling conditions

(norma _/ sio al or disease states) modulated or mediated by MM -trticularly those where elevated levels of MMP have be' detected.

All forms of arthritis (i.e., arthritic diseases soft tissue rheumatism, polychondritis and tendonitis) are characterized by the erosion of the articular cartilage of affected joints. Since cartilage consists primarily of proteoglycans and type II collagen, proteinases capable of attacking both macromolecules have been implicated in the progression of the diseases [Harris et al. , (1969) Arthri tis Rheum. 12, 92-102;

Harris et al. , (1970) Arthritis Rheum. 13, 83-95; Woolley et al., (1977) Arthri tis Rheum. 20, 1231-1239; and Krane, S.M., (1981) Ann . Rheum . Dis . 40, 433-448].

The metastasis of tumor cells is a process that is inhibited by the connective tissue barriers of the host. The association of both interstitial collagenases and proteinases capable of degrading type IV collagen found in basement membrane is well documented and is believed to facilitate metastasis [Strauli et al. , (1980) Proteinases and Tumor Invasion, Raven Press, New York;

Liotta et al . , (1991) Tumor Invasion and Metastasis , pp. 319-333, Martinus Nijhoff, Dordrecht; Blood, CH. and Zetter, B.R., (1990) Biochim . Biophys . Acta 1032, 89-118; Liotta et al. , (1983) Lab . Invest . 49, 636-649; and Liotta et al. , (1980) Nature (London) 284, 67-68].

Periodontal disease is an inflammatory disease that is triggered by bacteria that inhabit the gingival cavity. Periodontis is characterized by the progressive loss of the attachment apparatus of teeth. Since the major protein component of gingival tissue and bone is type I collagen, collagenases are believed to participate in the progression of the disease [Robertson, P.B. and Simpson, J. (1976) J ^" . Periodontol . , 47, 29-33; and Birkedal-Hansen, H., (1980) in Collagenases in Normal and Pathological Connective Tissue, (Woolley, D.E. and

Evanson, J.M., eds), pp. 127-140, Wiley and Sons, New York] .

Corneal ulceration can be brought about by chemical or thermal burns, infections Stevens-Johnson syndrome, Mooren's ulcer, vitamin A deficiency, and many other diseases. The corneal stroma is composed pre¬ dominantly of type I collagen that is progressively degraded during ulceration [Van Wart, H.E. and Mookhtiar, K.A. (1990) in Biological Response Modifiers for Tissue Repair (Grotendorst, G., Jhelmeland, L.M. and Gills, J.P., eds), pp. 23-50, Portfolio, The Woodlands, TX;

Brown et al. , (1969) Arch . Ophthalmol . 81, 370-373; and Berman, M.B. (1980) in Collagenases in Normal and Pathological Connective Tissue, (Woolley, D.E. and Evanson, J.M., eds), pp. 141-174, Wiley and Sons, New York] .

Glomerular diseases, such as proteinuria, dytrophobic epidermolysis bullosa, which a separation of the dermis and epidermis, are believed to be influenced by collagenases [Eisen, A.Z. (1969) J. Invest . Dermatol . 52, 449-453] .

Bone resorption diseases, such as osteoporosis, Paget's disease, hyperparathyroidism and cholesteatoma, are believed to involve the action of collagenases [Vaes, G. (1980) in Collagenases in Normal and Pathological Connective Tissue, (Woolley, D.E. and Evanson, J.M., eds), pp. 185-207, Wiley and Sons, New York; Gardner et al., (1971) Surg. Forum, 22, 435-437; Abramson, M. (1969) Ann . Otol . Rhinol . Laryngol . , 78, 112-124; Sakamoto et al., (1975) Biochem. Biophys . Res . Commun . 63, 172-177; Griffith et al. , (1965) J. Am. Med. Assoc . 193, 91-94; and Eeckhout et al. , (1986) Biochem. J. 239 793-796]. Certain MMP have been reported as mediating ovulation and implantation, thus inhibition of these MMP would provide a means of birth control [Librach et al. , J. Cell Biol . , 13, 437-449, 1991; and Brannstrόm et al. , Endocrinology, 122, 5, 1715-1721, 1988].

Certain MMP have been associated with angiogenesis relating to tumor growth or to the neo¬ vascularization associated with diabetic retinopathy and macular degeneration. Inhibition of theεa MMP would provide a means of slowing or halting the development of such conditions [Moses et al. , Bio/ technology, 9, 630- 634, 1991; and Langer et al. , Proc . Natl . Aca . Sci . USA, 77, 7, 4331-4335, 1980)] . MMP have been linked with coronary thrombosis caused by atherosclerotic plaque rupture [Henney et al. ,

Proc . Natl . Acad. Sci . , 88, 8154-8158, 1991] . Inhibition of MMP could alleviate this condition.

Interstitial collagenase has been implicated as a possible etiological agent in the emphysema disease process. Although elastase has been proposed as the primary enzyme responsible for emphysematous lung damage, there is evidence that other extra-ceullular matrix proteases could play a role in emphysema [D'Armiento et al., Cell , 71, 955-961 December 11, 1992] .

TESTING

The potency and selectivity of compounds of the present invention as inhibitors of MMP are determined by assay against MMPs that are associated with the metabolic turnover of interstitial collagens in the extracellular matrix of humans. For example, following the procedures described in Example 21, or modifications thereof.

Five types of MMP are assayed, i.e., fibro- blast-type collagenase (HFC) , gelatinase (HFG) and stromelysin (HFS) and neutrophil-type collagenase (HNC) and gelatinase (HNG) .

The assay method is based on the hydrolysis of DNP-Pro-Leu-Ala-Leu-Trp-Ala-Arg as the substrate (according to Netzel-Arnett, S.; Mallya, S. K. ; Nagase, H.; Birkedal-Hansen, H.; Van Wart, H. E. Anal. Biochem . 1991, 195, 86-92) .

ADMINISTRATION

The compounds of this invention are adminis- tered at a therapeutically effective dosage, i.e., that amount which, when administered to a mammal in need thereof, is sufficient to effect treatment, as described above (for example, to reduce or otherwise treat inflammation, pain and/or pyrexia in the mammal) . Administration of the active compounds and salts

described herein can be via any of the accepted modes of administration for agents that serve similar utilities.

The level of the drug in a formulation can vary within the full range employed by those skilled in the art, e.g., from about 0.01 percent weight (%w) to about 99.99%w of the drug based on the total formulation and about .01%w to 99.99%w excipient . Preferably the drug is present at a level of about 10%w to about 70%w.

Generally, an acceptable daily dose is of about 0.001 to 50 mg per kilogram body weight of the recipient per day, preferably about 0.05 to 25 mg per kilogram body weight per day, and most preferably about 0.01 to 10 mg per kilogram body weight per day. Thus, for adminis¬ tration to a 70 kg person, the dosage range would be about 0.07 mg to 3.5 g per day, preferably about 3.5mg to 1.75 g per day, and most preferably about 0.7 mg to 0.7 g per day depending upon the individuals and disease state being treated. Such use optimization is well within the ambit of those of ordinary skill in the art. Administration can be via any accepted systemic or local route, for example, via parenteral, oral (particularly for infant formulations) , intravenous, nasal, bronchial inhalation (i.e., aerosol formulation) , transdermal or topical routes, in the form of solid, semi-solid or liquid dosage forms, such as for example, tablets, suppositories, pills, capsules, powders, solutions, suspensions, aerosols, emulsions or the like, preferably in unit dosage forms suitable for simple administration of precise dosages. The compositions will include a conventional pharmaceutical carrier or excipient and an active compound of Formula I and, in addition, may include other medicinal agents, pharma¬ ceutical agents, carriers, adjuvants, etc. Carriers can be selected from the various oils, including those of petroleum, animal, vegetable or synthetic origin, for example, peanut oil, soybean oil, mineral oil, sesame

oil, and the like. Water, saline, aqueous dextrose, and glycols are preferred liquid carriers, particularly for injectable solutions. Suitable pharmaceutical carriers include starch, cellulose, talc, glucose, lactose, sucrose, gelatin, malt, rice, flour, chalk, silica gel, magnesium stearate, sodium stearate, glycerol mono- stearate, sodium chloride, dried skim milk, glycerol, propylene glycol, water, ethanol, and the like. Other suitable pharmaceutical carriers and their formulations are described in "Remington 's Pharmaceutical Sciences" by E. W. Martin.

If desired, the pharmaceutical composition to be administered may also contain minor amounts of non-toxic auxiliary substances such as wetting or emulsifying agents, pH buffering agents and the like, such as for example, sodium acetate, sorbitan mono- laurate, triethanolamine oleate, etc.

The compounds of this invention are generally administered as a pharmaceutical composition which comprises a pharmaceutical excipient in combination with a compound of Formula I. The level of the drug in a formulation can vary within the full range employed by those skilled in the art, e.g., from about .01 percent weight (%w) to about 99.99%w of the drug based on the total formulation and about .01%w to 99.99%w excipient. Preferably, the formulation will be about 3.5 to 60% by weight of the pharmaceutically active compound, with the rest being suitable pharmaceutical excipients.

INTRAVENOUS ADMINISTRATION

Intravenous injection has proven to be an important route of administration for therapeutic agents. The compounds of the present invention can be adminis¬ tered via this route, for example, by dissolving the compound, ester, ether or salt in a suitable solvent

(such as water or saline) or incorporation in a liposomal

formulation followed, by dispersal into an acceptable infusion fluid. A typical daily dose of a compound of the invention can be administered by one infusion, or by a series of infusions spaced over periodic intervals.

ORAL ADMINISTRATION

Oral administration can be used to deliver the compound of Formula I using a convenient daily dosage regimen which can be adjusted according to the degree of affliction or for renal impairment, or to compensate for the toxic effects of other medications administered con¬ temporaneously. For such oral administration, a pharmaceutically acceptable, non-toxic composition is formed by the incorporation of any of the normally employed excipients, such as, for example, pharmaceutical grades of mannitol, lactose, starch, magnesium stearate, sodium saccharine, talcum, cellulose, glucose, gelatin, sucrose, magnesium carbonate, and the like. Such compositions take the form of solutions, suspensions, tablets, pills, capsules, powders, sustained release formulations and the like. Such compositions may contain between .01 wt/wt% and 99.99 wt/wt% of the compound of Formula I, but preferably such compositions will contain between 25 wt/wt% and about 80 wt/wt%. Preferably the compositions will take the form of a capsule, pill or tablet and thus the composition will contain, along with the active ingredient, a diluent such as lactose, sucrose, dicalcium phosphate, and the like; a disintegrant such as starch or derivatives thereof; a lubricant such as magnesium stearate and the like; and a binder such as a starch, polyvinylpyrro- lidone, gum acacia, gelatin, cellulose and derivatives thereof, and the like. For oral administration to infants, a liquid formulation (such as a syrup or suspension) is preferred.

LIPOSOMAL FORMULATIONS

Pharmaceutical formulations based on liposomes have recently reached human clinical trials. Their benefits are believed related to favorable changes in tissue distribution and pharmacokinetic parameters that result from liposome entrapment of drugs, and may be applied to the compounds of the present invention by those skilled in the art.

The formulations can be designed to either target drug to disease sites [see: Lopez-Berestein et al., J. Infect . Dis . , 151 : 704-710 (1985) ; Gotfredsen et al., Biochemical Pharmacology, 32 : 3389-3396 (1983)] ; or to the reticuloendothelial system [see Eppstein et al . , Jnt. J. Immunotherapy, 2 : 115-126 (1986)], to increase duration of drug action [see: Gabizon et al . , Cancer

Res . , 42 : 4734 (1982) ; Eppstein et al . , -Deliverv -Systems for Peptide Drugs, Eds. S.S. Davis, L. Ilium and E. Tomlinson, Plenum Pub. Corp., New York, pp. 277-283; CA. Hunt, Biochemi ca et Biophysica Acta . , 719 : 450-463 (1982); and. Senior et al. , Biochemica et Biophysica

Acta . , 839 : 1-8 (1985)], or to divert a drug away from organs that are particularly sensitive to its toxic effects [see: Weinstein et al . , Pharmac . Ther. , 24 : 207-233 (1983) ; Olson et al . , Eur . J. Cancer Clin . Oncol . , 18 : 167-176 (1982); and Gabzion et al. , supra . ] . Controlled release liposomal liquid pharmaceutical formulations for injection or oral administration are described in U.S. Patent No. 4,016,100. Liposomal applications for oral drug delivery of a lyophilized liposome/peptide drug mixture filled into intestine capsules have also been suggested, see U.S. Patent No. 4,348,384. The foregoing are incorpor¬ ated herein by reference.

SUPPOSITORIES

For systemic administration via suppository, traditional binders and carriers include, for example, polyalkaline glycol or triglycerides [e.g., PEG 1000 (96%) and PEG 4000 (4%)]. Such suppositories may be formed from mixtures containing active ingredients in the range of from about 0.5 wt/wt% to about 10 wt/wt%; preferably from about 1 wt/wt% to about 2 wt/wt%.

LIQUIDS

Liquid pharmaceutically administrable compositions can, for example, be prepared by dissolving, dispersing, etc. an active compound (about 0.5% to about 20%) , as described above, and optional pharmaceutical adjuvants in a carrier, such as, for example, water, saline, aqueous dextrose, glycerol, ethanol and the like, to thereby form a solution or suspension.

Actual methods of preparing such dosage forms are known, or will be apparent, to those skilled in this art; for example, see Remington 's Pharmaceutical

Sciences, Mack Publishing Company, Easton, Pennsylvania, 16th Ed., 1980. The composition to be administered will, in any event, contain a quantity of the active compound(s) in a pharmaceutically effective amount for relief of the particular condition being treated in accordance with the teachings of this invention.

EXAMPLES

The following examples are given to enable those skilled in the art to more clearly understand and to practice the present invention. They should not be considered as limiting the scope of the invention, but merely as being illustrative and representative thereof.

EXAMPLE 1

PREPARATION OF (4-5,5- )-3-(1-OXO- -METHYLPENTYL)- 4-METHYL-5-PHENYL-2-OXAZOLIDINONE IA. Formula 2 Where R ² Is 2-Methylpropyl

To a solution of ( 4S, 5R) -4-methyl-5-phenyl-2- oxazolidinone (9.7 g) [prepared according to the procedures in Evans, D. A.; Mathre, D. J.; Scott, W. L. J. Org. Chem. 1985, 50 , 1830-1835] in 100 mL of THF cooled to -78°C with stirring, was added 35.6 mL of n- butyllithium (1.6 M in hexane) until the orange-red color of the di-anion just persisted. The reaction mixture was then treated with 7.0 mL (7.7 g) of distilled 4-methyl- pentanoyl chloride, warmed to 0°C and stirred for 30 min. Then 20 mL of 1 M aqueous K ₂ C0 ₃ was added and the resultant two-phase mixture was stirred at 25°C for 1 h. The organic solvents were evaporated in vacuo and the residue was extracted twice with CH ₂ C1 ₂ . The combined organic extract was washed with water and brine, then was dried over Na ₂ S0 ₄ and evaporated. The residue was subjected to flash chromatography on silica gel (hexane- EtOAc) to give 14.5 g (97% yield) of (45, 5-R) -3- (l-oxo-4- methylpentyl) -4-methyl-5-phenyl-2-oxazolidinone as a solid. Characteristic analytical data are as follows: mp 76-77°C (hexane/EtOAc) ; ^α H NMR (300 MHz, CDC1 ₃ ) δ 7.30- 7.46 (m, 5H) , 5.67 (d, J=8 Hz, IH) , 4.77 (p, J=7 Hz, IH) , 2.88-3.05 (m, 2H) , 1.53-1.69 (m, 3H) , 0.94 (d, J=7 Hz, 6H) , 0.90 (d, J=7 Hz, 3H) ; [α] ²⁵ _D -33° (c = 1.07, CH ₂ C1 ₂ ) .

IB. Formula 2 Varying R ²

By following the procedures described in Example IA and substituting 4-methylpentanoyl chloride with other compounds of Formula 1 (where the R ² substituents are as indicated in the table below) there are obtained the corresponding substituted compounds of Formula 2.

where (CA) is ( 45, 5-R) -4-methyl-5-phenyl-2 -oxazolidinone

R ² Name methyl ( 45, 5-R) -3 - ( l-oxo-propyl ) -4 -methyl -5- phenyl-2 -c: azolidinone

2-propyl (45, 5-R) -3- (l-oxo-3-methylbutyl) -4- methyl-5-phenyl-2-oxazolidinone

2-butyl (45, 5-R) -3-(l-oxo-3-methylpentyl) -4- methyl-5-phenyl-2-oxazolidinone benzyl (45, 5-R) -3- (l-oxo-3-phenylpropyl) -4- methyl-5-phenyl-2-oxazolidinone blocked 4- (45, 5R) -3- [l-oxo-3- (4-

-hydroxyphenylm hydroxypheny1)propyl] -4-methyl-5■ ethyl phenyl-2-oxazolidinone blocked 3- (45, 5R) -3- [l-oxo-3- (3-indolyl) - indolylmethyl propyl] -4-methyl-5-phenyl-2- oxazolidinone

(45, 5R) -3- [l-oxo-3- (4- methoxypheny1- methoxyphenyl)propyl] -4-methyl ^■ methyl phenyl-2-oxazolidinone phenylethyl (45, 5J?)-3-(l-oxo-4-phenylbutyl) -4- methyl-5-phenyl-2-oxazolidinone blocked 4- (45, 5R) -3- (l-oxo-6-aminohexyl) -4- aminobutyl methyl-5-phenyl-2-oxazolidinone blocked (45, 5R) -3- (l-oxo-5-guanylpentyl) -4- 3-guanylpropyl methyl-5-phenyl-2-oxazolidinone blocked (45, 5R) -3- [l-oxo-3- (4-imidazoyl) -

4-imidazoyl- propyl] -4-methyl-5-phenyl-2- methyl oxazolidinone methylthioethyl ;45, 5R) -3- [l-oxo-4 (methylthio)butyl] 4-methyl-5-phenyl-2-oxazolidinone

EXAMPLE 2

PREPARATION OF (45,5-R)-3- [ (25) -l-OXO-2- ( (BENZYLTHIO)METHYL)-4-METHYLPENTYL] - 4-METHYL-5-PHENYL-2-OXAZOLIDINONE

2A. Formula 3 Where R ² Is 2-Methylpropyl

A solution of lithium diisopropy1amide was prepared from 1.96 mL (1.42 g) of diisopropylamine and 7.6 mL of n-butyllithium (1.75 M in hexane) in 14 mL of THF. The solution was cooled to -78°C with stirring, combined with a solution of (45, 5R) -3- (l-oxo-4- methylpentyl) -4-methyl-5-phenyl-2-oxazolidinone (3.50 g) in THF (14 mL) and stirred for 30 min at -78°C. To this mixture was added 2.1 mL (3.1 g) of benzyl bromomethyl sulfide. The reaction mixture was stirred for 2 h at -25°C and for 2 h at 0°C. Aqueous NH ₄ C1 solution was added to the mixture. The organic solvents were removed in vacuo and the residue was extracted twice with CH ₂ C1 ₂ . The combined organic extract was washed twice with 1 M aqueous NaHS0 ₄ , then with saturated NaHC0 ₃ , brine, dried over Na ₂ S0 ₄ and evaporated. The residue was subjected to flash chromatography on silica gel (hexane-EtOAc) to give 5.0 g (96% yield) of (45, 5R) -3- [ (25) -l-oxo-2- ( (benzylthio)methyl) -4-methylpentyl] -4-methyl-5-phenyl-2- oxazolidinone as a clear oil. Characteristic analytical data are as follows: ^X H NMR (300 MHz, CDC1 ₃ ) δ 7.24-7.47 (m, 10H) , 5.66 (d, J=7 Hz, IH) , 4.81 (p, J=7 Hz, IH) , 4.31-4.36 (m, IH) , 3.80 (AB q, J=13 Hz, 2H) , 2.72 (dd, J=9, 14 Hz, IH) , 2.52 (dd, J=5, 14 Hz, IH) , 1.35-1.71 (m,

3H) , 0.89-0.95 (m, 9H) ; [α] ²⁵ _D -91° (c = 2.60, CH ₂ C1 I '

2B. Formula 3 Varying R ²

By following the procedures described in Example 2A and substituting (45,5-R) -3- (l-oxo-4- methylpentyl) -4-methyl-5-phenyl-2-oxazolidinone with other compounds of Formula 2 (where the R ² substituents are as indicated in the table below) there are obtained the corresponding substituted compounds of Formula 3.

C

where (CA) is (45,5-R) -4-methyl-5-phenyl-2-oxazolidinone

R ² Name methyl (45,5-R)-3-{ (25)l-oxo-2-[ (benzylthio) - methyl] ropyl)-4-methyl-5-pheny1-2- oxazolidinone

2-propyl (45,5-R) -3-{ (25) l-oxo-2-[ (benzylthio) methyl] -3-methylbutyl}-4-methyl-5- phenyl-2-oxazolidinone

2-butyl (45,5J?)-3-{ (2-S)l-oxo-2- [ (benzylthio) • methyl] -3-methylpentyl}-4-methyl-5- phenyl-2-oxazolidinone benzyl (45,5-R)-3-{ (25)l-oxo-2-[ (benzylthio) - methyl] -3-phenylpropyl}-4-methyl-5- phenyl-2-oxazolidinone blocked 4- (45,5-R)-3-{ (25)l-oxo-2-[ (benzylthio) - hydroxy- methyl]-3-(4-hydroxyphenyl)propyl}-4- phenylmethyl methyl-5-phenyl-2-oxazolidinone blocked 3- (4S,5-R)-3-{ (25)l-oxo-2-[ (benzylthio) - indolyl- methyl] -3-(3-indolyl)propyl}-4-methyl-5- methyl pheny1-2-oxazolidinone

Formula 3

where (CA) is |45, 5-R) -4-methyl-5-phenyl-2-oxazolidinone

Name

4-methoxy- (45, 5-R) -3-{ (25) l-oxo-2- [ (benzylthio) - phenylmethyl methyl] -3- (4-methoxyphenyl)propyl}-4- methyl-5-phenyl-2-oxazolidinone phenylethyl (45, 5R) -3 - { (25)l-oxo-2-[ (benzylthio) - methyl] -4-phenylbuty1}-4-methyl-5- phenyl-2-oxazolidinone blocked 4- (45, 5-R)-3-{ (25)1-0X0-2- [ (benzylthio) - aminobutyl methyl] -6-aminohexy1}-4-methy1-5-pheny1- 2-oxazolidinone blocked (45, 5-R)-3-{ (25)l-oxo-2-[ (benzylthio) -

3-guanyl- methyl] -5-guanylpentyl}-4-methyl-5- propyl phenyl-2-oxazolidinone blocked (45, 5R) -3 - { (25)l-oxo-2-[ (benzylthio) -

4-imidazoyl- methyl] -3 (4-imidazoyl)propyl}-4-methyl- methyl 5-phenyl-2-oxazolidinone methyl- (4S,5-R)-3-{ (25)l-oxo-2- t (benzylthio) - thioethyl methyl] -4- (methylthio)butyl}-4-methyl-5- phenyl-2-oxazolidinone

EXAMPLE 3

PREPARATION OF BENZYL (25) -2- [ (BENZYLTHIO)METHYL] -

4-METHYLPENTANOATE

3A. Formula 4 Where R ² Is 2-Methylpropyl A solution of lithium benzyloxide was prepared by . ombining 2.5 mL (2.63 g) of benzyl alcohol with 50 mL of THF and 10.4 m of n-butyllithium (1.75 M in hexane) . This solution was combined with 5.0 g of (45, 5i?) -3- [ (25) - l-oxo-2- [ (benzylthio)methyl] -4-methylpentyl] -4-methyl-5- phenyl-2-oxazolidinone in 12 mL of THF over 30 min with stirring at -10°C The reaction mixture was warmed to 0°C and stirred for 1.5 h. It was then quenched by addition of half-saturated aqueous NH ₄ C1. The organic solvents were removed by evaporation in vacuo and the residue was extracted twice with CH ₂ C1 ₂ . The combined organic extract was washed with water and brine, dried over Na ₂ S0 ₄ and evaporated. The residue was subjected to flash chromatography on silica gel (hexane-EtOAc) to 'give 3.4 g (82% yield) of benzyl (25) -2- [ (benzylthio)methyl] - 4-methylpentanoate as an oil. Characteristic analytical data are as follows: ^J H NMR (300 MHz, CDC1 ₃ ) δ 7.23-7.39 (m, 10H) , 5.15 (AB q, J=13 Hz, 2H) , 3.69 (s, 2H) , 2.63- 2.69 (m, 2H) , 2.47-2.52 (m, IH) , 1.26-1.58 (m, 3H) , 0.86 (d, J=8 Hz, 3H) , 0.84 (d, J=9 Hz, 3H) .

3B. Formula 4 Varying R ²

By following the procedures described in Example 3A and substituting (45, 5-R) -3- [ (25) -l-oxo-2- [ (benzylthio) ethyl] -4-methylpentyl] -4-methyl-5-phenyl-2- oxazolidinone with other compounds of Formula 3 (where the R ² substituents are as indicated in the table below) there are obtained the corresponding substituted compounds of Formula 4.

Name methyl benzyl (25) -2- [ (benzylthio)methyl] proprionate

2-propyl benzyl (25) -2- [ (benzylthio)methyl] 3-methylbutanoate

2-butyl benzyl (25) -2- [ (benzylthio)methyl] 3-methylpentanoate benzyl benzyl (25) -2- [ (benzylthio)methyl] 3-phenylproprionate blocked 4- benzyl (25) -2- [ (benzylthio)methyl]

-hydroxy- 3- (4-hydroxyphenyl)proprionate phenylmethyl blocked 3- benzyl (25) -2- [ (benzylthio) ethyl] indolyl- 3- (3-indolyl) -proprionate methyl

4-methoxy- benzyl (25) -2- [ (benzylthio)methyl] phenylmethyl 3- (4-methoxypheny1)proprionate phenylethyl benzyl (25) -2- [ (benzylthio)methyl] 4-phenylbutanoate blocked 4- benzyl (25) -2- [ (benzylthio)methyl] aminobutyl 6-aminohexanoate blocked benzyl (25) -2- [ (benzylthio)methyl]

3-guanyl- 5-guanylpentanoate propyl blocked benzyl (25) -2- [ (benzylthio) ethyl]

4-imidazoyl- 3- (4-imidazoyl)proprionate methyl methyl- benzyl (25) -2- [ (benzylthio)methyl] thioethyl 4- (methylthio)butanoate

EXAMPLE 4 PREPARATION OF BnS-(CH ₂ -I»-Leu) -OH 4A. Formula 5 Where R ² Is 2-Methylpropyl A solution of 3.4 g of benzyl (25) -2-

[ (benzylthio)methyl] -4-methylpentanoate in 10 mL of a 30% solution of anhydrous hydrogen bromide in glacial acetic acid was stirred at 50°C for 30 min. The reaction mixture was cooled to room temperature, diluted with 20 mL of water and extracted twice with CH ₂ C1 ₂ . The combined extracts were evaporated under reduced pressure. The residue was dissolved in 50 mL of toluene and the solution was again evaporated. This procedure was repeated 2 more times in order to remove acetic acid. The crude product was dissolved in 1 M aqueous KOH and extracted with ether. The aσueous layer was acidified to pH 1 with concentrated HC1 a: extracted twice with CH ₂ C1 ₂ . The combined organic extracts were dried over Na ₂ S0 ₄ and evaporated to give 2.0 g (80% yield) of BnS- (CH ₂ -L-Leu) -OH as an oil. An analytical sample was obtained by Kugelrohr distillation at 150°C (0.01 torr) . Characteristic analytical data are as follows: ^* H NMR (300 MHz, CDC1 ₃ ) δ 7.25-7.33 (m, 5H) , 3.74 (s, 2H) , 2.48- 2.72 (m, 3H) , 1.53-1.61 (m, 2H) , 1.33-1.36 (m, IH) , 0.89 (d, J=6 Hz, 3H) , 0.87 (d, J=7 Hz, 3H) ; mass spectrum

(Cl), m/e 253 (MH\ 100), 235 (MH ⁺ - H ₂ 0, 77) ; [α] ²⁵ _D -42° (c = 0.6, MeOH) . Anal. Calcd for C ₁₄ H ₂₀ O ₂ S: C, 66.63; H, 7.99; S, 12.70. Found: C, 66.91; H, 7.93; S, 13.13.

4B. Formula 5 Varying R ²

By following the procedures described in Example 4A and substituting benzyl (25) -2- [ (benzylthio)methyl] -4- ethylpentanoate with other compounds of Formula 4 (where the R ² substituents are as indicated in the table below) there are obtained the corresponding substituted compounds of Formula 5.

Formula 5

0

s

R ² Name methyl BnS- (CH ₂ -L-Ala) -OH

2-propyl BnS- (CH ₂ -L-Val) -OH

2-butyl BnS- (CH ₂ -L-Ile) -OH benzyl BnS- (CH ₂ -L-Phe) -OH blocked 4-hydro- BnS-(CH ₂ -L-Tyr) -OH xyphenylmethyl blocked 3- BnS- (CH ₂ -L-Trp) -OH indolylmethyl

4-methoxyphenyl- BnS- (CH ₂ -L- (Tyr-OCH ₃ ) ) -OH methyl phenylethyl BnS- (CH ₂ -L-Phet) -OH blocked 4-aminobutyl BnS- (CH ₂ -L-Lys) -OH blocked 3-guanyl- BnS- (CH ₂ -L-Arg) -OH propyl blocked 4-imidazoyl- BnS- (CH ₂ -L-His) -OH methyl methylthioethyl BnS- (CH ₂ -L-Met) -OH

EXAMPLE 5 PREPARATION OF Phe-Ala-NH ₂

5A. Preparation Of Formula 6

1. Formula 6 Where R ³ Is Benzyl and R ⁴ Is -CH(R )-C(0)NH ₂ Where R ⁵ Is Methyl

The following procedure for the preparation of the amino acid residue is a modification of the procedures reported in Bodanszky, M. ; Bodanszky, A. The Practice of Peptide Synthesis ; Springer-Verlag: New York, 1984; p 129-142. A mixture of 5.00 g of Cbz-Phe-OH (Sigma Chemical Company), 2.12 g of N-hydroxysuccinimide and 3.79 g of 1, 3-dicyclohexylcarbodiimide in 20 mL of dry THF was kept at 4°C under N ₂ for 26 h. The resulting precipitate was removed by filtration. To the filtrate was added an aqueous solution of 1.79 g of Ala-OH containing 0.802 g of NaOH. The mixture was stirred at room temperature for 18 h. The solid was removed by filtration and the filtrate was diluted with saturated aqueous NaHC0 ₃ and extracted with CHC1 ₃ . The aqueous layer was acidified with 1 M HC1 and the resulting precipitate was collected by filtration, washed with water, and dried under vacuum to give 6.00 g (97% yield) of Cbz-Phe-Ala-OH, which was used without further purification. Characteristic analytical data are as follows: mp 153-154°C; H NMR (300 MHz, CDC1 ₃ - CD ₃ OD) δ 7.38-7.10 (m, 10H, 2xPh) , 6.91-6.80 (br, IH, H-N) , 5.60- 5.52 (br, IH, H-N), 5.03 (s, 2H, CH ₂ -Bn) , 4.45-4.38 (m, 2H, Hα-(Phe)+Hα-(Ala) ) , 3.01 (m, 2H, CK ₂ -(Phe)), 1.35 (d, J=7 Hz, 3H, CH ₃ -(Ala) ) .

The above described procedure was repeated using 5.65 g of Cbz-Phe-Ala-OH and excess anhydrous ammonia in THF to obtain 7.16 g of crude Cbz-Phe-Ala-NH ₂ . The crude product was then subjected to hydrogenolysis in MeOH over 10% Pd/C to afford 3.42 g (95% yield) of Phe- Ala-NH ₂ as a yellow solid after flash chromatography

(EtOAc-MeOH, 10:1) . Characteristic analytical data are as follows: mp 95-97°C; R _F 0.10 (1:2 MeOH:EtOAc) ; ^* H NMR (300 MHz, CDC1 ₃ ) δ 7.80-7.72 (br, IH, H-N) , 7.35-7.18 (m, 5H, Ph) , 6.58-6.45 (br, IH, H-N), 5.70-5.60 (br, IH, H- N) , 4.46 (quint, J=7 Hz, IH, HOC- (Ala)) , 3.62 (dd, J=4, 9 Hz, IH, Hα-(Phe)), 3.21 (dd, J=4, 11 Hz, IH, Hβ-(Phe)) , 2.72 (dd, J=9, 11 Hz, IH, Hβ-(Phe) ), 1.35 (d, J=7 Hz, 3H, CH ₃ ) ; ¹³ C NMR (75 MHz, CDC1 ₃ ) δ 175.3 (C=0) , 174.8 (C=0) , 137.6 (C-Ph) , 129.4 (CH-Ph) , 128.8 (CH-Ph) , 127.0 (CH- Ph) , 56.0 (CH-α) , 48.0 (CH-α) , 40.5 (CH ₂ ) , 17.7 (CH ₃ ) ; [α] ²⁵ _D -25.6° (c=1.90, EtOH) .

2. Formula 6 Where R ³ Is 3-indolylmethyl and R ⁴ Is Benzyl The coupling procedure described above for the preparation of Cbz-Phe-Ala-NH ₂ was employed using 10.0 g of Cbz-Trp-OH (Sigma Chemical Company) and 4.84 mL of freshly distilled BnNH ₂ in dry THF to give 11.3 g (89% yield) of crystalline Cbz-Trp-NHBn. Characteristic analytical data are as follows: mp 104-105°C; ^X H NMR (300 MHz, CDC1 ₃ ) δ 8.00-6.88 (m, 16H, Ar) , 5.85 (br, IH, H-α) , 5.45 (br, IH, H-N), 5.10 (s, 2H, CH ₂ -0) , 4.57-4.45 (br, H-), 4.27 (t, 6H, CH ₂ -N) , 3.38 (dd, J=4, 14 Hz, IH, H-β), 3.16 (dd, J=8, 14 Hz, IH, H-β); mass spectrum (El), m/e 427 (M ⁺ ), 336 (M ⁺ -Bn) , 277 (M ⁺ -Cbz-NH) , 130 (M ⁺ -Cbz-NH-CH- CONH-Bn) , 91 (Bn ⁺ ) ; [OC] ²⁵ _D +7.0° (c=0.20, EtOAc) .

A solution of 2.20 g (5.15 mmol) Cbz-Trp-NHBn in 60 mL of MeOH was subjected to hydrogenolysis over Pd/C to give 1.43 g (4.88 mmol, 95%) of Trp-NHBn. Characteristic analytical data are as follows: mp 112- 114°C; ^α H NMR (300 MHz, CDC1 ₃ ) δ 8.40-7.00 (m, 11H, Ar) , 4.44 (d, J=6 Hz, 2H, CH ₂ -N) , 3.77 (dd, J=4 Hz, 9H, H-α), 3.42 (dd, J=4, 14 Hz, IH, H-β), 2.98 (dd, J=9, 14 Hz, IH, H-β); mass spectrum (El), m/e 293 (M ⁺ ) , 277 (M ⁺ -NH ₂ ) , 130 (M ⁺ -H ₂ N-CH-CONH-Bn) ; [α] ²⁵ _D +42.1° (c=1.02, MeOH) .

3. Formula 6 Where R ³ Is Benzyl and R ⁴ Is Methyl

A mixture of 1.00 g of Cbz-Phe-OH (Sigma

Chemical Company), 0.385 g of W-hydroxysuccinimide and 0.541 g of 1,3-dicyclohexylcarbodiimide in 5 mL of dry

THF was kept at 4°C under N ₂ overnight. The resulting precipitate was removed by filtration, and to the filtrate was added excess 40% aqueous methylamine at room temperature. The mixture was stirred at room temperature for 30 min. The solid was removed by filtration and the filtrate was partitioned between saturated aqueous NaHC0 ₃ and CH ₂ C1 ₂ . The organic layer was separated, dried over anhydrous Na ₂ S0 _4, and evaporated under reduced pressure to give 1.12 g of the- crude Cbz-Phe-NHMe. To a solution of the crude product in 30 mL of

MeOH was added 0.21 g of 10% Pd/C and H ₂ was bubbled through the mixture via a dispersion tube until TLC analysis showed completion of the hydrogenolysis (3 h) . The catalyst was removed by filtration and the solvent was evaporated under reduced pressure. The residue was partitioned between CHC1 ₃ and water and the aqueous layer was acidified with 1 M HC1 to approximately pH 2 and was extracted with CHC1 ₃ (3x) . The aqueous layer was then neutralized with 10% NaOH and again was extracted with CHC1 ₃ (3x) . The latter organic extract was dried over anhydrous Na ₂ S0 ₄ and was evaporated under reduced pressure to give 0.554 g (93% yield) of Phe-NHMe. Characteristic analytical data are as follows: mp 48-50°C; R _F 0.10 (EtOAc) ; *H NMR (300 MHz, CDC1 ₃ ) δ 7.34-7.20 (m, 5H, Ph) , 3.60 (dd, J=4, 9 Hz, IH, H-α), 3.30 (dd, J=4, 11 Hz, IH,

H-β) , 2.81 (d, 5H, CH ₃ -N) , 2.65 (dd, J=9, 11 Hz, IH, H-β) ; ¹³ C NMR (75 MHz, CDCl ₃ ) δ 175.6 (C=0) , 138.6 (C-Ph) , 129.9 (CH-Ph), 129.3 (CH-Ph), 127.3 (CH-Ph), 56.8 (CH ₃ ) , 41.3 (CH) , 26.0 (CH ₂ ); mass spectrum (PCI), m/e 179 (M+l) ; [α] ²⁵ _D +8.5° (c=4.20, EtOH) .

5B. Formula 6 Varying R ³ , R ⁴ and R ⁵

By following the procedures described in Example 5A and substituting CBz-Phe-OH and Ala-OH with other compounds of Formulae 6A and 6B, respectively [where the substituents R ³ (Formula 6A) , and R ⁴ and R ⁵ (Formula 6B) are as indicated in the table below] there are obtained the correspondingly substituted compounds of Formula 6.

Formula 6

R ³

where R ⁴ is metllyl or benzyl

R ³ R ⁴ Name hydrogen -CH ₃ Gly-CH ₃ methyl -Bn Ala-Bn

2-propyl -CH ₃ Val-CH ₃

2-butyl -Bn Leu-Bn

2-methylpropyl -CH ₃ Ile-CH ₃ blocked 4-aminobutyl -Bn Lys-Bn blocked 3-guanylpropyl -CH ₃ Arg-CH ₃ blocked 4-imidazoylmethyl -Bn His-Bn benzyl -CH ₃ Phe-CH ₃ blocked 4-hydroxyphenyl- -Bn Tyr-Bn methyl blocked 3-indolylmethyl -CH ₃ Trp-CH ₃

4-methoxyphenyl ethy1 -Bn (Tyr-OCH ₃ ) -Bn phenylethyl -CH ₃ (Phet) -CH ₃

Formula 6

where R ⁴ is -CH- (R ⁵ ) -C (0)NH ₂

R ³ R ⁵ Name hydrogen methyl Gly-Ala-NH ₂ methyl 2-butyl Ala-Ile-NH ₂

2-propyl 2-methylpropyl Val-Leu-NH ₂

2-butyl hydrogen Leu-Gly-NH ₂

2-methylpropyl 2-propyl Ile-Val-NH ₂ blocked 4-aminobutyl 2-methylpropyl Lys-Leu-NH ₂ blocked 3- methyl Arg-Ala-NH ₂ guanylpropyl blocked 4-imidazoyl- 2-butyl His-Ile-NH ₂ methyl benzyl 2-methylpropyl Phe-Leu-NH ₂ blocked 4-hydroxy- hydrogen Tyr-Gly-NH ₂ phenylmethyl bl cked 3- 2-propyl Trp-Val-NH ₂ indolylmethyl -methoxyphenylmethy1 2-methylpropyl (Tyr-OCH ₃ ) - Leu-NH ₂ phenylethyl methyl (Phet)-Ala-NH ₂ hydrogen blocked 4- Gly-Lys-NH ₂ aminobutyl methyl blocked 3- Ala-Arg-NH ₂ guanylpropyl

2-propyl 2-imidazoyl- Val-His-NH ₂ methyl

2-butyl blocked 4- Leu-Lys-NH ₂ aminobutyl

2-methylpropyl blocked 3- Ile-Arg-NH ₂ guanylpropyl

Formula 6 R ³

0

where R ⁴ is -CH- (R ⁵ ) -C (0)NH ₂

R ³ R ⁵ Name blocked 4-aminobutyl 2-imidazoyl- Lys-His-NH ₂ methyl blocked 3- blocked 4- Arg-Lys-NH ₂ guanylpropyl aminobutyl blocked 4-imidazoyl- blocked 3- His-Arg-NH ₂ methyl guanylpropyl benzyl 2-imidazoyl- Phe-His-NH ₂ methyl blocked 4-hydroxy- blocked 4- Tyr-Lys-NH ₂ phenyImethy1 aminobutyl blocked 3- blocked 3- Trp-Arg-NH ₂ indolylmethyl guanylpropyl -methoxyphenyImethy1 2-imidazoyl- (Tyr-OCH ₃ ) - methyl His-NH ₂ phenylethyl blocked 4- (Phet)-Lys-NH ₂ a inobutyl hydrogen thiolmethyl Gly-Cys-NH ₂ methyl methylthioethyl Ala-Met-NH ₂

2-propyl hydroxymethyl Val-Ser-NH ₂

2-butyl 1-hydroxyethyl Leu-Thr-NH ₂

2-methylpropyl thiolmethyl Ile-Cys-NH ₂ blocked 4-aminobutyl methylthioethyl Lys-Met-NH ₂ blocked 3- hydroxymethyl Arg-Ser-NH ₂ guanylpropyl blocked 4-imidazoyl- 1-hydroxyethyl His-Thr-NH ₂ methyl benzyl thiolmethyl Phe-Cys-NH ₂

Formula 6

where R ⁴ is -CH- (R ⁵ ) -C (0)NH ₂

R ³ R ⁵ Name blocked 4-hydroxy- methylthioethyl Tyr-Met-NH ₂ phenylmethyl blocked 3- hydroxymethyl Trp-Ser-NH ₂ indolylmethyl

4-methoxyphenyImethy1 1-hydroxyethyl (Tyr-OCH ₃ ) - Thr-NH ₂ phenylethyl hydroxymethyl (Phet)-Ser-NH ₂ blocked 3- methylthioethyl Arg-Met-NH ₂ guanylpropyl

EXAMPLE 6

PREPARATION OF HS-(CH ₂ -L-Leu) -Phe-Ala-NH ₂

6A. Preparation of Formula 7 Where R ² Is 2-Methylpropyl, R ³ Is Benzyl and R ⁴ Is Methyl

BnS- (CH ₂ -L-Leu) -OH and Phe-Ala-NH ₂ were coupled using the procedure described in the Bodanszky reference

(i.e., Bodanszky, M., Bodanszky, A. The Practice of

Peptide Synthesis ; Springer-Verlag: New York, 1984, p

145) to give the S-benzyl peptide, i.e., BnS-(CH ₂ -L-Leu) -

Phe-Ala-NH ₂ as a glass in 78% yield. Characteristic analytical data are as follows: ^λ E NMR (300 MHz, CDC1 ₃ ) δ

7.21-7.35 ( , 10H) , 6.63 (d, J=8 Hz, IH) , 6.23 (br s,

IH) , 5.85 (d, J=7 Hz, IH) , 5.25 (br s, IH) , 4.42-4.57 (m,

2H) , 3.65 (s, 2H) , 3.23 (dd, J=7, 14 Hz, IH) , 3.04 (dd,

J=8, 14 Hz, IH) , 2.47-2.50 (m, 2H) , 2.22-2.26 (m, IH) , 1.11-1.40 ( , 3H) , 1.32 (d, J=7 Hz, 3H) , 0.76 (d, J=9 Hz,

3H) , 0.74 (d, J=9 Hz, 3H) .

6B. Preparation of Formula 8 Where R ² Is 2-Methylpropyl, R ³ Is Benzyl and R ⁴ Is Methyl

Debenzylation of BnS- (CH ₂ -L-Leu) -Phe-Ala-NH ₂ was done using the Na and liquid ammonia procedure of Evans and co-workers (Evans, D. A.; Mathre, D. J.; Scott, W. L.

J. Org. Chem. 1985, 50, 1830-1835) to give HS- (CH ₂ -L-Leu) -

Phe-Ala-NH ₂ in 89% yield after flash chromatography with

CHCl ₃ -EtOH. Characteristic analytical data are as follows: mp 217-219°C; H NMR (CDC1 ₃ ) δ 7.1-7.3 (m, 5H) , 6.72 (m, IH) , 6.25 (m, IH) , 6.08 (br s, IH) , 5.38 (br s, IH) , 4.71 (q, J=7 Hz, IH) , 4.44 (p, J=7 Hz, IH) , 3.20 (dd, J=7, 13 Hz, IH) , 3.07 (dd, J=7, 13 Hz, IH) , 2.50- 2.70 (m, 2H) , 2.30-2.41 (m, IH) , 1.20-1.50 (m, 3H) , 1.34 (d, J=7 Hz, 3H) , 0.87 (d, J=7 Hz, 3H) , 0.83 (d, J=7 Hz, 3H) ; mass spectrum (Cl) , m/e 380 (MH ⁺ , 100) ; [α] ²⁵ _D -24° (c = 0.35, MeOH) .

6C. Formula 8 Where R ² Is 2-Methylpropyl Varying R ³ , R ⁴ and R ⁵ By following the procedures described in

Examples 6A and 6B and substituting Phe-Ala-NH ₂ with other compounds of Formula 6 (e.g., compounds with R ³ , R ⁴ and R ⁵ that are prepared according to Example 5A and exemplified in Example 5B) there are obtained the correspondingly substituted of compounds of Formula 8.

Formula 8

where R ⁴ is methyl or benzyl

R ² R ³ R ⁴

2-methylpropyl hydrogen -CH ₃

2-methylpropyl methyl -Bn

2-methylpropyl 2-propyl -CH ₃

2-methylpropyl 2-butyl -Bn

2-methylpropyl 2-methylpropyl -CH ₃

2-methylpropyl blocked 4-aminobutyl -Bn

2-methylpropyl blocked 3-guanylpropyl -CH ₃

2-methylpropyl blocked 4-imidazoylmethyl -Bn

2-methylpropyl benzyl -CH ₃

2-methylpropyl blocked 4-hydroxyphenyl- -Bn methyl

2-methylpropyl 3-indoyImethy1 -CH ₃

2-methylpropyl 4-methoxyphenyImethy1 -Bn

2-methylpropyl phenylethyl -CH ₃

Formula 8

where R ⁴ is -CH- (R ⁵ ) -C (0)NH ₂

R ² R ³ R ⁵

2-methylpropyl hydrogen methyl

2-methylpropyl methyl 2-butyl

2-methylpropyl 2-propyl 2-methylpropyl

2-methylpropyl 2-butyl hydrogen

2-methylpropyl 2-methylpropyl 2-propyl

2-methylpropyl blocked 4- 2-methylpropyl aminobutyl

2-methylpropyl blocked 3- methyl guanylpropyl

2-methylpropyl blocked 4- 2-butyl imidazoylmethyl

2-methylpropyl benzyl 2-methylpropyl

2-methylpropyl blocked 4-hydro- hydrogen xyphenyImethy1

2-methylpropyl 3-indoylmethyl 2-propyl

2-methylpropyl 4-methoxypheny1- 2-methylpropyl methyl

2-methylpropyl phenylethyl methyl

2-methylpropyl hydrogen blocked 4- aminobutyl

2-methylpropyl methyl blocked 3- guanylpropyl

2-methylpropyl 2-propyl blocked 4- imidazoylmethyl

2-methylpropyl 2-butyl blocked 4- aminobutyl

Formula 8

where R ⁴ is -CH- (R ⁵ ) -C (0)NH ₂

R ² R ³ R ⁵ -methylpropyl 2-methylpropyl blocked 3- guanylpropyl

2-methylpropyl blocked 4- blocked 4- aminobutyl imidazoylmethyl

2-methylpropyl blocked 3- blocked 4- guanylpropyl aminobutyl

2-methylpropyl blocked 4- blocked 3- imidazoyImethy1 guanylpropyl

2-methylpropyl benzyl blocked 4- imidazoyImethy1

2-methylpropyl blocked 4-hydro- blocked 4- xyphenyImethy1 aminobutyl

2-methylpropyl 3-indoyImethy1 blocked 3- guanylpropyl

2-methylpropyl 4-methoxypheny1- blocked 4- methyl imidazoylmethyl

-methylpropyl phenylethyl blocked 4- aminobutyl

2-methylpropyl hydrogen thiolmethyl

2-methylpropyl methyl methylthioethyl

2-methylpropyl 2-propyl hydroxymethyl

2-methylpropyl 2-butyl 1-hydroxyethyl

2-methylpropyl -methylpropyl thiolmethyl

2-methylpropyl blocked 4- methylthioethyl aminobutyl

2-methylpropyl blocked 3- hydroxymethyl guanylpropyl

Formula 8

where R ⁴ is -CH- (R ⁵ ) -C (0)NH ₂

R ² R ³ R ⁵

2-methylpropyl blocked 4- 1-hydroxyethy1 imidazoylmethyl

2-methylpropyl benzyl thiolmethyl

2-methylpropyl - - blocked 4-hydro- methylthioethyl xyphenylmethyl

2-methylpropyl 3-indoylmethyl hydroxymethyl

2-methylpropyl 4-methoxypheny1- 1-hydroxyethy1 methyl

2-methylpropyl phenylethyl hydroxymethyl

6D. Formula 8 Varying R ² , R ³ , R ⁴ and R ^s

By following the procedures described in Examples 6A and- 6B and substituting Bns- (CH ₂ -L-Leu) -OH with other compounds of Formula 5 (e.g., compounds with R ² that are prepared according to Example 4A and exemplified with Example 4B) and Phe-Ala-NH ₂ with other compounds of Formula 6 (e.g., compounds with R ³ , R ⁴ and R ⁵ that are prepared according to Example 5A and exemplified with Example 5B) there are obtained the following correspondingly substituted compounds of Formula 8.

Formula 8

where R ⁴ is methyl or benzyl

R ² R ³ R ⁴

4-methoxyphenyl hydrogen -CH ₃ blocked 4-hydroxy- methyl -Bn phenyl benzyl 2-propyl -CH ₃

2-butyl 2-butyl -Bn

2-butyl 2-methylpropyl -CH ₃ benzyl blocked 4-aminobutyl -Bn blocked 4-hydroxy- blocked 3-guanylpropyl -CH ₃ phenyl

4-methoxyphenyl blocked 4-imidazoyl- -Bn methyl

4-methoxyphenyl benzyl -CH ₃ blocked 4-hydroxy- blocked -Bn phenyl 4-hydroxyphenyImethy1 benzyl 3-indoyImethy1 -CH ₃

2-butyl 4-methoxyphenylmethy1 -Bn

2-butyl phenylethyl -CH ₃

Formula 8

where R ⁴ is -CH-(R ⁵ ) -C(0)NH ₂

R ² R ³ R ⁵ benzyl hydrogen methyl blocked methyl 2-butyl 4-hydroxypheny1

4-methoxyphenyl 2-propyl 2-methylpropyl

2-butyl hydrogen

4-methoxyphenyl 2-methylpropyl 2-propyl blocked blocked 4- 2-methylpropyl 4-hydroxypheny1 aminobutyl benzyl blocked 3- methyl guanylpropyl

2-butyl blocked 4- 2-butyl i idazoylmethyl

2-butyl benzyl 2-methylpropyl benzyl blocked hydrogen

4-hydroxypheny1- methyl blocked 3-indoyImethy1 2-propyl 4-hydroxypheny1

4-methoxyphenyl 4-methoxyphenyl- 2-methylpropyl methyl

4-methoxyphenyl phenylethyl methyl blocked hydrogen blocked 4- 4-hydroxypheny1 aminobutyl benzyl methyl blocked 3- guanylpropyl

2-butyl 2-propyl blocked 4- imidazoyl- methyl

Formula 8

where R ⁴ is -CH- (R ⁵ ) -C(0)NH ₂

R ² R ³ R ⁵

2-butyl 2-butyl blocked 4- aminobutyl benzyl 2-methylpropyl blocked 3- guanylpropyl blocked blocked 4- blocked 4- -hydroxypheny1 aminobutyl imidazoyl- ethyl -methoxyphenyl blocked 3- blocked 4- guanylpropyl aminobutyl

4-methoxyphenyl blocked 4- blocked 3- imidazoylmethyl guanylpropyl blocked benzyl blocked 4- 4-hydroxypheny1 imidazoyl- methyl benzyl blocked blocked 4-

4-hydroxypheny1- aminobutyl methyl

2-butyl 3-indoyImethy1 blocked 3- guanylpropyl

2-butyl 4-methoxypheny1- blocked 4- methyl imidazoyl- methyl benzyl phenylethyl blocked 4- aminobutyl blocked hydrogen thiolmethyl 4-hydroxypheny1

4-methoxyphenyl methyl methylthio- ethyl

2-butyl 2-propyl hydroxymethyl benzyl 2-butyl 1-hydroxyethy1

Formula 8

where R ⁴ is -CH- (R ⁵ ) -C (0)NH ₂

R ² R ³ R ⁵ blocked 2-methylpropyl thiolmethyl 4-hydroxyphenyl

4-methoxyphenyl blocked 4- methylthio- aminobutyl ethyl blocked blocked 3- hydroxymethyl 4-hydroxyphenyl guanylpropyl benzyl blocked 4- 1-hydroxyethy1 imidazoylmethyl

2-butyl benzyl thiolmethyl

2-butyl blocked methylthio-

4-hydroxyphenyl- ethyl methyl benzyl 3-indoyImethy1 hydroxymethyl blocked 4-methoxyphenyl- 1-hydroxyethy1 4-hydroxyphenyl methyl

4-methoxyphenyl phenylethyl hydroxymethyl

EXAMPLE 7 PREPARATION OF MeS-(CH ₂ -L-Leu)-Phe-Ala-NH ₂

7A. Formula 10 Where R ¹ Is CH ₃ , R ² Is 2-Methylpropyl, R ³ Is Benzyl and R ⁴ Is Methyl

To 143 mg of HS- (CH ₂ -L-Leu) -Phe-Ala-NH ₂ under nitrogen was added a solution of 10 mg of Na in 1.6 mL of MeOH, followed by 35 μL of CH ₃ I. The mixture was stirred at 40°C for 6 h, then the solvent was evaporated. The residue was partitioned between water and EtOAc and the aqueous layer was extracted twice with EtOAc. The combined organic layers were dried over Na ₂ S0 ₄ and

evaporated, and the residue was purified by flash chromatography on silica gel to give 113 mg (76% yield) of Me-S- (CH ₂ -L-Leu) -Phe-Ala-NH ₂ as a white solid. Characteristic analytical data are as follows: mp 191- 193°C; ^J H NMR (300 MHz, CDCl ₃ -CD ₃ OD) δ 7.24-7.33 ( , 5H) , 4.65 (t, J=8 Hz, IH) , 4.37 (q, J=8 Hz, IH) , 3.16 (dd, J=7, 14 Hz, IH) , 3.04 (dd, J=7, 14 Hz, IH) , 2.48-2.60 (m, 3H) , 2.05 (s, 3H) , 1.2-1.55 (m, 3H) , 1.32 (d, J=7 Hz, 3H) , 0.86 (d, J=7 Hz, 3H) , 0.82 (d, J=7 Hz, 3H) ; mass spectrum (El), m/e 393 (M ⁺ , 35); [α] ²⁵ ₅₄₆ -39° (c = 0.29, MeOH) .

7B. Formula 10 Where R ¹ Is n-butyl

By following the procedure described in Example 7A and substituting n-butyl iodide for methyl iodide, n- Bu-S- (CH ₂ -L-Leu, -Phe-Ala-NH ₂ was obtained as a solid. Characteristic analytical data are as follows: mp 180- 183°C; ^X NMR (300 MHz, CDC1 ₃ ) δ 7.21-7.36 (m, 5H) , 6.79 (d, J=8 Hz, IH, NH) , 6.30 (br s, IH, NH) , 6.08 (d, J=6 Hz, IH, NH) , 5.31 (br s, IH, NH) , 4.58 (q, J=6 Hz, IH) , 4.47 (p, J=7 Hz, IH) , 3.25 (dd, J=6, 14 Hz, IH) , 3.07 (dd, J=8, 14 Hz, IH) , 2.66 (dd, J=5, 13 Hz, IH) , 2.42-2.5 (m, 4H), 1.38-1.56 (m, 7H) , 1.34 (d, J=7 Hz, 3H) , 0.91 (t, J=7 Hz, 3H) , 0.85 (d, J=7 Hz, 3H) , 0.79 (d, J=6 Hz, 3H) ; mass spectrum (Cl) , m/e 436 (MH ⁺ , 100) ; [α] ⁵ ₅₄₆ -41° (c = 0.4, MeOH) .

7C. Formula 10 Varying R ¹ , R ² , R ³ , R ⁴ and R ^s

By following the procedures described in Examples 7A and substituting CH ₃ I with other compounds of Formula 9 (e.g., compounds with R ¹ ) and HS- (CH ₂ -L-Leu) - Phe-Ala-NH ₂ with other compounds of Formula 8 (e.g., compounds with R ² , R ³ , R ⁴ and R ⁵ that are prepared according to Example 6A and exemplified in Example 6B) there are obtained the correspondingly substituted of compounds of Formula 10.

Formula 10

where R ⁴ is methyl or benzyl

R ¹ R ² R ³ R ⁴ ethyl 4-methoxy¬ hydrogen -CH ₃ phenyl benzyl blocked methyl -Bn 4-hydroxy¬ phenyl phenethyl benzyl 2-propyl -CH ₃ blocked 2-butyl 2-butyl -Bn 4-hydroxy- phenethyl blocked 2- (4- 2-butyl 2-methyl¬ -CH ₃ imidazolyl) - propyl ethyl blocked benzyl blocked 4- -Bn -carboxypropyl aminobutyl ethyl blocked blocked 3- -CH ₃ 4-hydroxy¬ guanylpropyl phenyl benzyl 4-methoxy¬ blocked 4- -Bn phenyl imidazoyl- methyl phenethyl 4-methoxy¬ benzyl -CH ₃ phenyl blocked blocked blocked -Bn

4-hydroxy- 4-hydroxy¬ 4-hydroxy¬ phenethyl phenyl phenylmethyl blocked 2- (4- benzyl 3-indoyl- -CH ₃ imidazolyl) - methyl ethyl blocked 2-butyl 4-methoxy¬ -Bn 3-carboxypropyl phenylmethyl ethyl 2-butyl phenylethyl -CH ₃

Formula 10

where R ⁴ is -CH-(R ⁵ ) - (0)NH ₂

R ¹ R ² R ³ R ⁵ benzyl 2-butyl hydrogen methyl phenethyl benzyl methyl 2-butyl blocked blocked 2-propyl 2-methyl¬ 4-hydroxy- 4-hydroxy¬ propyl phenethyl phenyl blocked 2- (4- 4-methoxy¬ 2-butyl hydrogen imidazolyl) - phenyl ethyl blocked 4-methoxy¬ 2-methyl¬ 2-propyl 3-carboxy¬ phenyl propyl propyl ethyl blocked blocked 2-methyl¬ 4-hydroxy¬ aminobutyl propyl phenyl benzyl benzyl blocked 3- methyl guanyl- propyl phenethyl 2-butyl blocked 4- 2-butyl imidazoyl- methyl blocked 2-butyl benzyl 2-methyl¬

4-hydroxy- propyl phenethyl blocked 2- (4- benzyl blocked hydrogen imidazolyl) - 4-hydroxy¬ ethyl phenyl- methyl blocked blocked 3-indoyl- 2-propyl 3-carboxy¬ 4-hydroxy¬ methyl propyl phenyl

Formula 10

where R ⁴ is -CH-(R ⁵ ) - C(0)NH ₂

R ¹ R ² R ³ R ⁵ ethyl 4-methoxy¬ 4-methoxy¬ 2-methyl¬ phenyl phenyl- propyl methyl benzyl 4-methoxy¬ phenylethyl methyl phenyl phenethyl blocked hydrogen blocked 4- 4-hydroxy¬ aminobutyl phenyl blocked benzyl methyl blocked 3- -hydroxyphen- guanylpropyl ethyl blocked 2- (4- 2-butyl 2-propyl blocked 4- imidazolyl) - imidazoyl- ethyl methyl blocked 2-butyl 2-butyl blocked 4- 3-carboxy¬ aminobutyl propyl ethyl benzyl 2-methyl- blocked 3- propyl guanylpropyl benzyl blocked blocked 4- blocked 4- 4-hydroxy¬ aminobutyl imidazoyl- phenyl methyl phenethyl 4-methoxy¬ blocked 3- blocked 4- phenyl guany1- aminobutyl propyl blocked 4-methoxy¬ blocked 4- blocked 3- -hydroxyphen- phenyl imidazoyl- guanylpropyl ethyl methyl blocked 2- (4- blocked benzyl blocked 4- imidazolyl) - 4-hydroxy¬ imidazoyl- ethyl phenyl methyl

Formula 10

where R ⁴ is -CH- (R ⁵ ) -C (0)NH ₂

R ¹ R ² R ³ R ⁵ blocked benzyl blocked blocked 4- 3-carboxy¬ 4-hydroxy¬ aminobutyl propyl phenyl- methyl ethyl 2-butyl 3-indoyl- blocked 3- methyl guanylpropyl benzyl 2-butyl 4-methoxy¬ blocked 4- phenyl- imidazoyl- methyl methyl phenethyl benzyl phenylethyl blocked 4- aminobutyl blocked blocked hydrogen thiolmethyl -hydroxyphen- 4-hydroxy¬ ethyl phenyl bloc i 2- (4- 4-methoxy¬ methyl methylthio- imidazolyl) - phenyl ethyl ethyl blocked 2-butyl 2-propyl hydroxy¬ 3-carboxy¬ methyl propyl ethyl benzyl 2-butyl 1-hydroxy- ethyl benzyl blocked 2-methyl¬ thiolmethyl 4-hydroxy¬ propyl phenyl phenethyl 4-methoxy¬ t ocked 4- methylthio- phenyl aminobutyl ethyl blocked blocked blocked 3- hydroxy¬

4-hydroxyphen- 4-hydroxy¬ guanyl- methyl ethyl phenyl propyl

Formula 10

where R ⁴ is -CH-(R ⁵ )- C(0)NH ₂

R ¹ R ² R ³ R ⁵ blocked 2- (4- benzyl blocked 4- 1-hydroxy- imidazolyl) - imidazoyl- ethyl ethyl methyl blocked 2-butyl benzyl thiolmethyl 3-carboxy¬ propyl ethyl 2-butyl blocked methylthio- 4-hydroxy¬ ethyl phenyl- methyl benzyl benzyl 3-indoyl- hydroxy¬ methyl methyl phenethyl blocked 4-methoxy¬ 1-hydroxy- 4-hydroxy¬ phenyl- ethyl phenyl methyl blocked 4-methoxy¬ phenylethyl hydroxy¬ 4-hydroxy- phenyl methyl phenethyl

EXAMPLE 8 PREPARATION OF Me-(-R5) -SO-(CH ₂ -L-Leu) -Phe-Ala-NH ₂

8A. Formula 11 Where R ¹ Is CH ₃ , R ² Is 2-Methylpropyl, R ³ Is Benzyl and R ⁴ Is Methyl

To a cold (-10°C) solution of 50 mg of Me-S-

(CH ₂ -L-Leu) -Phe-Ala-NH ₂ in 4 mL of CH ₂ C1 ₂ and 2 mL of MeOH was added 25 mg of m-chloroperbenzoic acid. The reaction mixture was stirred at -10°C for 8 h. The solvents were removed by evaporation and the residue was triturated several times with ether. The residue was purified by

flash chromatography on silica gel (CHCl ₃ -EtOH eluent) to give 40 mg (77% yield) of Me- (RS) -SO- (CH ₂ -L-Leu) -Phe-Ala- NH ₂ as a mixture of two diastereomers. Characteristic analytical data are as follows: Η NMR (300 MHz, CDC1 3 CD ₃ OD) δ 7.20-7.35 ( , 5H) , 4.68 (m, IH) , 4.43 (m, IH) , 3.12-3.26 (m, IH) , 2.80-3.05 (m, 2H) , 2.56-2.70 (m, 2H) , 2.53 & 2.48 (s, 3H) , 1.25-1.68 (m, 3H) , 1.34 & 1.32 (d, J=7 Hz, 3H) , 0.82-0.91 (m, 6H) .

8B. Formula 11 Where R ¹ n-Butyl, R ² Is 2-Methylpropyl, R ³ Is Benzyl and R ⁴ Is Methyl

Following the procedure described in Example 8A and substituting n-Bu-S- (CH ₂ -L-Leu) -Phe-Ala-NH ₂ for Me-S-

(CH ₂ -L-Leu) -Phe-Ala-NH ₂ , n-Bu- [RS) -SO- (CH ₂ -L-Leu) -Phe-Ala- NH ₂ was obtained as a mixture of two diastereomers.

8C. Formula 11 Varying R ^x , R ² , R ³ , R ⁴ and R ⁵

By following the procedures described in Examples 8A and substituting MeS- (CH ₂ -L-Leu) -Phe-Ala-NH ₂ with other compounds of Formula 10 (e.g., compounds with R ¹ , R ² , R ³ , R ⁴ and R ⁵ that are prepared according to Examples 7A and 7B, and exemplified in Example 7C) there are obtained the correspondingly substituted of compounds of Formula 11.

Formula 11

where R ⁴ is methyl or benzyl

R ¹ R ² R ³ R ⁴ ethyl 4-methoxy¬ hydrogen -CH ₃ phenyl benzyl blocked methyl -Bn 4-hydroxy¬ phenyl phenethyl benzyl 2-propyl -CH ₃ blocked 2-butyl 2-butyl -Bn -hydroxyphen- ethyl blocked 2- (4- 2-butyl 2-methylpropyl -CH ₃ imidazolyl) - ethyl blocked benzyl blocked 4- -Bn 3-carboxy¬ aminobutyl propyl ethyl blocked blocked 3- -CH ₃ 4-hydroxy¬ guanylpropyl phenyl benzyl 4-methoxy¬ blocked 4- -Bn phenyl imidazoyl- methyl phenethyl 4-methoxy¬ benzyl -CH ₃ phenyl blocked blocked blocked -Bn

4-hydroxyphen- 4-hydroxy¬ 4-hydroxy¬ ethyl phenyl phenylmethyl

Formula 11

where R ⁴ is methyl or benzyl

R ¹ R' R ³ R ⁴ blocked 2- (4- benzyl 3-indoyImethy1 -CH ₃ imidazolyl) - ethyl blocked 2-butyl 4-methoxy¬ -Bn 3-carboxy¬ phenylmethyl propyl ethyl 2-butyl phenylethyl -CH ₃

Formula 11

where R ⁴ is -CH-(R ⁵ )- C(0)NH ₂

R ¹ R ² R ³ R ⁵ benzyl 2-butyl hydrogen methyl phenethyl benzyl methyl 2-butyl blocked blocked 2-propyl 2-methyl¬ 4-hydroxy- 4-hydroxy¬ propyl phenethyl phenyl blocked 2- (4- 4-methoxy¬ 2-butyl hydrogen imidazolyl) - phenyl ethyl blocked 4-methoxy¬ 2-methyl¬ 2-propyl 3-carboxy¬ phenyl propyl propyl ethyl blocked blocked 4- 2-methyl¬ 4-hydroxy¬ aminobutyl propyl phenyl benzyl benzyl blocked 3- methyl guanyl- propyl phenethyl 2-butyl blocked 4- 2-butyl imidazoyl- methyl blocked 2-butyl benzyl 2-methyl¬ 4-hydroxy¬ propyl phenethyl blocked 2- (4- benzyl blocked hydrogen imidazolyl) - 4-hydroxy¬ ethyl phenyl- methyl blocked blocked 3-indoyl- 2-propyl 3-carboxy¬ 4-hydroxy¬ methyl propyl phenyl

Formula 11

where R ⁴ is -CH-(R ⁵ ) - 2(0)NH ₂

R ¹ R ² R ³ R ⁵ ethyl 4-methoxy¬ 4-methoxy¬ 2-methyl¬ phenyl phenyl- propyl methyl benzyl 4-methoxy¬ phenyl¬ methyl phenyl ethyl phenethyl blocked hydrogen blocked 4- 4-hydroxy¬ aminobutyl phenyl blocked benzyl methyl blocked 3- 4-hydroxy- guanylpropyl phenethyl blocked 2- (4- 2-butyl 2-propyl blocked 4- imidazolyl) - imidazoyl- ethyl methyl blocked 2-butyl 2-butyl blocked 4- 3-carboxy¬ aminobutyl propyl ethyl benzyl 2-methyl¬ blocked 3- propyl guanylpropyl benzyl blocked blocked 4- blocked 4- 4-hydroxy¬ aminobutyl imidazoyl- phenyl methyl phenethyl 4-methoxy¬ blocked 3- blocked 4- phenyl guanyl- aminobutyl propyl blocked 4-methoxy¬ blocked 4- blocked 3- 4-hydroxy- phenyl imidazoyl- guanylpropyl phenethyl methyl blocked 2- (4- blocked benzyl blocked 4- imidazolyl) - 4-hydroxy¬ imidazoyl- ethyl phenyl methyl

Formula 11

where R ⁴ is -CH- (R ⁵ ) -C (0)NH ₂

R ¹ R ² R ³ R ⁵ blocked benzyl blocked blocked 4- 3-carboxy¬ 4-hydroxy- aminobutyl propyl phenyl¬ methyl ethyl 2-butyl 3-indoyl- blocked 3- methyl guanylpropyl benzyl 2-butyl 4-methoxy¬ blocked 4- phenyl- imidazoyl- methyl methyl phenethyl benzyl phenyl¬ blocked 4- ethyl aminobutyl blocked blocked hydrogen thiolmethyl 4-hydroxy- 4-hydroxy¬ phenethyl phenyl blocked 2- (4- 4-methoxy¬ methyl methylthio- imidazolyl) - phenyl ethyl ethyl blocked 2-butyl 2-propyl hydroxy¬ 3-carboxy¬ methyl propyl ethyl benzyl 2-butyl 1-hydroxy- ethyl benzyl blocked 2-methyl¬ thiolmethyl 4-hydroxy¬ propyl phenyl phenethyl 4-methoxy¬ blocked 4- methylthio- phenyl aminobutyl ethyl blocked blocked blocked 3- hydroxy¬ 4-hydroxy- 4-hydroxy¬ guanyl- methyl phenethyl phenyl propyl

Formula 11

where R ⁴ is -CH-(R ⁵ ) - C(0)NH ₂

R ¹ R ² R ³ R ⁵ blocked 2- (4- benzyl blocked 4- 1-hydroxy- imidazolyl) - imidazoyl- ethyl ethyl methyl blocked 2-butyl benzyl thiolmethyl 3-carboxy¬ propyl ethyl 2-butyl blocked methylthio- 4-hydroxy¬ ethyl phenyl- methyl benzyl benzyl 3-indoyl- hydroxy¬ methyl methyl phenethyl blocked 4-methoxy¬ 1-hydroxy- 4-hydroxy¬ phenyl- ethyl phenyl methyl blocked 4-methoxy¬ phenyl¬ hydroxy¬

4-hydroxy- phenyl ethyl methyl phenethyl

EXAMPLE 9 PREPARATION OF Me- {RS) -SO(NH) -(CH ₂ -L-Leu)-Phe-Ala-NH ₂

9A. Formula I Where X Is 0, R ¹ Is CH ₃ , R ² s

2-Methylpropyl, R ³ Is Benzyl and R ⁴ Is Methyl

To a solution of 20 mg of Me- (RS) -SO- (CH ₂ -L-

Leu) -Phe-Ala-NH ₂ in 1.5 mL of THF was added 32 mg of O-mesitylsulfonylhydroxylamine. The resulting mixture was stirred at 25°C for 10 h [according to Johnson, C.

R.; Kirchhoff, R. A.; Corkins, H. G. J. Org. Chem. 1974,

39, 2458-2459] . The reaction mixture was partitioned between EtOAc and water. NaOH (10% aqueous) was added to

bring the aqueous layer to pH 9. The aqueous layer was extracted twice with EtOAc and the combined organic layers were dried over Na ₂ S0 ₄ and evaporated. The residue was purified by flash chromatography followed by preparative TLC on silica gel (CHCl ₃ -EtOH eluents) to give 6 mg (29% yield) of Me- (RS) -SO(NH) - (CH ₂ -L-Leu) -Phe-Ala-NH ₂ as a mixture of two diastereomers. Characteristic analytical data are as follows: ^: H NMR (300 MHz, CDC1 ₃ - CD ₃ OD) δ 7.23-7.34 (m, 5H) , 4.56-4.62 (m, IH) , 4.36-4.42 (m, IH) , 3.40-3.58 (m, IH) , 3.14-3.26 (m, IH) , 2.86-3.07 (m, 3H) , 2.75 & 2.71 (s, 3H) , 1.34 & 1.33 (d, J=7 Hz, 3H) , 1.21-1.56 (m, 3H) , 0.90 (d, J=7 Hz, 3H) , 0.85 (d, J=6 Hz, 3H) ; mass spectrum (PCI) , m/e 425 (MH ⁺ , 8), 346 (100) .

9B. Formula I Where X Is O, R ¹ Is -n-butyl, R ² Is 2-Methylpropyl, R ³ Is Benzyl and R ⁴ Is Methyl

Following the procedure described in Example 9A and substituting n-Bu-SO- (CH ₂ -L-Leu) -Phe-Ala-NH ₂ for Me- SO-(CH ₂ -L-Leu) -Phe-Ala-NH ₂ , n-Bu- ( RS) -SO(NH) - (CH ₂ -L-Leu) - Phe-Ala-NH ₂ was obtained as a mixture of two diastereomers. Characteristic analytical data are as follows: mp 141-144°C (lyophilized powder) ; ^X H NMR (300 MHz, CDC1 ₃ -CD ₃ 0D) δ 7.23-7.32 (m, 5H) , 4.51 (t, J=7 Hz, IH) , 4.35 (q, J=8 Hz, IH) , 2.8-3.3 (m, 7H) , 1.2-1.8 (m,

7H) , 1.33 (d, J=7 Hz, 1.5H) , 1.32 (d, J=7 Hz, 1.5H) , 0.96 (t, J=7 Hz, 3H) , 0.88 (d, J=6 Hz, 3H) , 0.84 (d, J=6 Hz, 3H) ; mass spectrum (Cl) , m/e 467 (MH ⁺ , 26) , 346 (MH ⁺ - Ji-BuSO(NH)H, 100) ; [α] ²⁵ ₅₄₆ -31° (c = 0.12, MeOH) .

9C. Formula I Varying R ¹ , R ² , R ³ R ⁴ and R ⁵

By following the procedures described in Examples 9A and 9B and substituting Me- (RS) -SO- (CH ₂ -L- Leu) -Phe-Ala-NH ₂ with other compounds of Formula 10 (e.g., compounds with R ¹ , R ² , R ³ , R ⁴ and R ⁵ that are prepared according to Examples 8A and 8B, and exemplified in

Example 8C) there are obtained the correspondingly substituted of compounds of Formula I.

Formula I

where R ⁴ is methyl or benzyl

R ¹ R ² R ³ R ⁴ ethyl 4-methoxy¬ hydrogen -CH ₃ phenyl benzyl 4-hydroxy¬ methyl -Bn phenyl phenethyl benzyl 2-propyl -CH ₃

4-hydroxyphen- 2-butyl 2-butyl -Bn ethyl

2-(4- 2-butyl 2-methylpropyl -CH ₃ imidazolyl) - ethyl

3-carboxypropyl benzyl 4-aminobutyl -Bn ethyl 4-hydroxy¬ 3-guanylpropyl -CH ₃ phenyl benzyl 4-methoxy¬ 4-imidazoyl- -Bn phenyl ethyl phenethyl 4-methoxy¬ benzyl -CH ₃ phenyl

4-hydroxyphen- 4-hydroxy¬ 4-hydroxyphenyl -Bn ethyl phenyl methyl

2-(4- benzyl 3-indoylmethyl -CH ₃ imidazolyl) - ethyl

3-carboxypropyl 2-butyl 4-methoxy¬ -Bn phenylmethyl ethyl 2-butyl phenylethyl -CH ₃

Formula I

where R ⁴ is -CH-(R ⁵ ) - C(0)NH ₂

R ¹ R ² R ³ R ⁵ benzyl 2-butyl hydrogen methyl phenethyl benzyl methyl 2-butyl

4-hydroxy- 4-hydroxy¬ 2-propyl 2-methyl¬ phenethyl phenyl propyl

2-(4- 4-methoxy¬ 2-butyl hydrogen imidazolyl) - phenyl ethyl

3-carboxy¬ 4-methoxy¬ 2-methyl¬ 2-propyl propyl phenyl propyl ethyl 4-hydroxy¬ 4-aminobuty1 2-methyl¬ phenyl propyl benzyl benzyl 3- methyl guanylpropyl phenethyl 2-butyl 4-imidazoyl- 2-butyl methyl

4-hydroxy- 2-butyl benzyl 2-methyl¬ phenethyl propyl

2-(4- benzyl 4-hydroxy¬ hydrogen imidazolyl) - phenylmethyl ethyl

3-carboxy¬ 4-hydroxy¬ 3-indoyl- 2-propyl propyl phenyl methyl ethyl 4-methoxy¬ 4-methoxy¬ 2-methyl¬ phenyl phenylmethyl propyl benzyl 4-methoxy¬ phenylethyl methyl phenyl phenethyl 4-hydroxy¬ hydrogen 4- phenyl aminobutyl

Formula I

where R ⁴ is -CH-(R ⁵ ) - C(0)NH ₂

R ¹ R ² R ³ R ⁵

4-hydroxy- benzyl methyl 3-guanyl- phenethyl propyl

2-(4- 2-butyl 2-propyl 4- imidazolyl) - imidazoyl- ethyl methyl

3-carboxy¬ 2-butyl 2-butyl 4- propyl aminobutyl ethyl benzyl 2-methyl¬ 3-guanyl- propyl propyl benzyl 4-hydroxy¬ 4-aminobutyl 4- phenyl imidazoyl- methyl phenethyl 4-methoxy¬ 3- 4- phenyl guanylpropyl aminobutyl

4-hydroxy- 4-methoxy¬ 4-imidazoyl- 3-guanyl- phenethyl phenyl ethyl propyl

2-(4- 4-hydroxy¬ benzyl 4- imidazolyl) - phenyl imidazoyl- ethyl methyl

3-carboxy¬ benzyl 4-hydroxy¬ 4- propyl phenylmethyl aminobutyl ethyl 2-butyl 3-indoyl- 3-guanyl- methyl propyl benzyl 2-butyl 4-methoxy¬ 4- phenylmethyl i idazoyl- methyl phenethyl benzyl phenylethyl 4- aminobutyl

4-hydroxy- 4-hydroxy¬ hydrogen thiolmethyl phenethyl phenyl

Formula I

where R ⁴ is -CH- (R ⁵ ) -C (0)NH ₂

R ¹ R ² R ³ R ⁵

2-(4- 4-methoxy¬ methyl methylthio- imidazolyl) - phenyl ethyl ethyl

3-carboxy¬ 2-butyl 2-propyl hydroxy¬ propyl methyl ethyl benzyl 2-butyl 1-hydroxy- ethyl benzyl 4-hydroxy¬ 2-methyl¬ thiolmethyl phenyl propyl phenethyl 4-methoxy¬ 4-aminobutyl methylthio- phenyl ethyl

4-hydroxy- 4-hydroxy¬ 3- hydroxy¬ phenethyl phenyl guanylpropyl methyl

2-(4- benzyl 4-imidazoyl- 1-hydroxy- imidazolyl) - methyl ethyl ethyl

3-carboxy¬ 2-butyl benzyl thiolmethyl propyl ethyl 2-butyl 4-hydroxy¬ methylthio- phenylmethyl ethyl benzyl benzyl 3-indoyl- hydroxy¬ methyl methyl phenethyl 4-hydroxy¬ 4-methoxy¬ 1-hydroxy- phenyl phenylmethyl ethyl

4-hydroxy- 4-methoxy¬ phenylethyl hydroxy¬ phenethyl phenyl methyl

EXAMPLE 10 PREPARATION OF ISOBUTYLMALONIC ACID 10A. Formula 12 Where R ² Is 2-Methylpropyl

To a solution of NaOEt prepared by dissolving 5.75 g (0.25 mol) of Na in 150 mL of absolute EtOH was added 39 mL (0.250 mol) of diethyl malonate with cooling in a water bath under N ₂ . To the resulting solution was added 24 mL (0.250 mol) of isobutyl bromide. The mixture was refluxed under N ₂ for 14 hours . The ethanol was evaporated under reduced pressure and the residue was partitioned between CHC1 ₃ and water. The aqueous Na ₂ S0 ₄ and evaporated. The residue was distilled under reduced pressure to give 47.2 g (0.219 mol, 88%) of diethyl isobutylmalonate as a colorless liquid. Characteristic analytical data are as follows: bp 127-135°C (25 mm Hg) ; ^r NMR (300 MHz, CDCL ₃ ) δ 4.20 (q, J=7 Hz, 4H) , 3,41 (t, J=8 Hz, IH) , 1.80 (t, J=7 Hz, 2H) , 1.57 (m, IH) , 1.27 (t, J=8 Hz, 6H) , 0.92 (d, J=7 Hz, 6H) .

To a solution of 8.34 g (38.6 mmol) of diethyl isobutylmalonate in 35 mL of 95% EtOH was added a solution of 9.0 g (161 mmol) of KOH in 110 mL of 95% EtOH. The mixture was stirred at 25°C for 16 hours and then at reflux for 1 hour. The mixture was cooled, diluted with water and extracted with CH ₃ C1. The aqueous layer was cooled to 0°C and acidified to pH<l with 30 mL of concentrated HCl, then it was extracted with CHC1 ₃ . The aqueous layer was further continuously extracted with CHC1 ₃ overnight. The combined organic extracts were dried over anhydrous Na ₂ S0 ₄ and evaporated to afford 5.44 g (33.9 mmol, 88%) of isobutylmaIonic acid as a solid which was essentially pure according to H NMR and which was used without further purification. Characteristic analytical data are as follows: mp 108-110°C; H NMR (300 MHz, CDC1 ₃ ) δ 3.52 (t, J=8 Hz, IH) , 1.84 (t, J=8 Hz, 2H) , 1.66 (m, IH) , 0.94 (d, J-7 Hz, 6H) ; ¹³ C NMR (75 MHz,

CDC1 ₃ ) δ 175 . 6 ( C=0 ) , 49 . 7 ( CH ) , 37 . 1 ( CH ₂ ) , 25 . 7 ( CH ) , 21 . 7 (CH ₃ ) .

10B. Formula 12 Where R ² Is 4-Methoxyphenylmethyl By following the procedures described in

Example 10A, crude diethyl 4-methoxyphenyImethylmalonate was obtained (100% yield) and converted to 4-methoxy- phenyImethylmalonic acid (69% yield) . Characteristic analytical data for the crude diethyl 4-methoxyphenyImethylmalonate are as follows: H NMR (300 MHz, CDC1 ₃ ) δ 7.13 (d, J=9 Hz, 2H) , 6.81 (d, J=9 Hz, 2H) , 4.13 (q, J=7 Hz, 4H) , 3.78 (s, 3H) , 3.60 (t, J=8 Hz, IH) , 3.16 (d, J=5 Hz, 2H) , 1.21 (t, J=7 Hz, 6H) . Characteristic analytical data for the 4-methoxyphenyl- methylmalonic acid are as follows: H NMR (300 MHz,

CDC1 ₃ ) δ 7.12 (d, J=9 Hz, 2H) , 6.79 (d, J=9 Hz, 2H) , 3.74 (s, 3H) , 3.58 (t, J=8 Hz, IH) , 3.48 (br s, 2H) , 3.14 (d, J=7 Hz, 2H) .

IOC. Formula 12 Varying R ²

By following the procedures described in Example 10A and substituting isobutyl bromide with other compounds of Formula 12A (where the R ² substituents are as indicated in the table below) there are obtained the corresponding substituted compounds of Formula 12.

Formula 12

R ² Name

H malonic acid methyl methylmalonic acid

2-propyl 2-propylmalonic acid

2-butyl 2-butylmalonic acid benzyl benzylmalonic acid blocked 4- 4-hydroxyphenyImethylmalonic acid hydroxyphenylmethyl blocked 3- 3-indolyImethylmalonic acid indolylmethyl phenylethyl phenylethylmalonic acid blocked 4- 4-aminobutylmalonic acid aminobutyl blocked 3- 3-guanylpropylmalonic acid guanylpropyl blocked 4- 4-imidazoylmethylmalonic acid imidazoylmethyl methylthioethyl methylthioethylmalonic acid

EXAMPLE 11 PREPARATION OF 4-METHYL-2-METHYLENEPENTANOIC ACID 11A. Formula 13 Where R ² Is 2-Methylpropyl

To a stirred mixture of 14.0 g of isobutylmalonic acid and 37 mL of 37% formalin was added 9.05 mL of diethylamine at room temperature. The mixture was stirred at room temperature for 3 h and refluxed for an additional 2 h. The reaction mixture was cooled to room temperature and diluted with CHC1 ₃ , and extracted with saturated aqueous NaHC0 ₃ . The aqueous layer was acidified with 1 M HC1 and extracted with CHC1 ₃ . The

organic extract was dried over anhydrous Na ₂ S0 ₄ and evaporated under reduced pressure to afford 11.0 g at 98% yield of 4-methyl-2-methylenepentanoic acid as a clear liquid which was used without further purification. Characteristic analytical data are as follows: ^X H NMR (300 MHz, CDC1 ₃ ) δ 6.32 (d, J=2 Hz, IH) , 5.62 (d, J=2 Hz, IH) , 2.17 (dd, J=l, 7 Hz, 2H) , 1.82 (m, IH) , 0.90 (d, J=7 Hz, 6H) ; ¹³ C NMR (300 MHz) δ 173.6, 139.4, 128.4, 40.7, 26.9, 22.0.

11B. Formula 13 Where R ² Is 4-Methoxyphenylmethyl

By following the procedures described in Example 11A and substituting isobutylmalonic acid with (4-methoxybenzyl)malonic acid, 5.6 g (58% yield) of 3- (4- methoxyphenyl) -2-methylenepropanoic acid was obtained. Characteristic analytical data are as follows: ^J H NMR (300 MHz, CDC1 ₃ ) δ 7.10 (d, J=9 Hz, 2H) , 6.82 (d, J=9 Hz, 2H) , 6.26 (br s, IH) , 5.48 (br s, IH) , 3.77 (s, 3H) , 3.54 (s, 2H) .

11C. Formula 13 Varying R ²

By following the procedures described in Example 11A and substituting isobutylmalonic acid with other compounds of Formula 12 (where the R ² substituents are as indicated in the table below) there are obtained the corresponding substituted compounds of Formula 13.

Name

H 2-propenoic acid methyl 2-methylenepropanoic acid

2-propyl 3-methyl-2-methylene-butanoic acid

2-butyl 3-methyl-2-methylene-pentanoic acid benzyl 3-phenyl-2-methylene-propanoic acid blocked 4- 3- (4-hydroxyphenyl) -2-methylene- hydroxypheny1- propanoic acid methyl blocked 3- 3- (3-indolyl) -2-methylene-propanoic indolylmethyl acid phenylethyl 4-phenyl-2-methylene-butanoic acid blocked 4- 6-amino-2-methylene-hexanoic acid aminobutyl blocked 3- 5-guanyl-2-methylene-pentanoic acid guanylpropyl blocked 4- 3- (4-imidazoyl) -2-methylene-propanoic imidazoyl- acid methyl

EXAMPLE 12 PREPARATION OF AcS-(CH ₂ -DL-Leu)-OH 12A. Formula 15 Where R ² Is 2-Methylpropyl

A mixture of 0.700 g of 4 ιethyl-2- methylenepentanoic acid and 0.90 mL (0.96 g) of thiolacetic acid was stirred under nitrogen for 26 h. The excess thiolacetic acid was removed by evaporation on the rotary evaporator with warming to give 1.10 g (99% yield) of AcS- (CH ₂ --DL-Leu) -OH. Characteristic analytical

data are as follows: mp 38-40°C [mp reported in literature mp 42-47°C (Sundeen, J. E.; Dejneka, T. U.S. Patent 4382081, 1983; Chem. Abstr. 1983, 98, 179923b) ; and mp 46-47°C (Darlak, K.; Miller, R. B.; Stack, M. S. Spatola, A. F.; Gray, R. D. J. Biol . Chem. 1990, 265, 5199-5205) ; ^l E NMR (300 MHz, CDC1 ₃ ) δ 3.14 (dd, J=6, 14 Hz, IH) , 2.97 (dd, J=9, 14 Hz, IH) , 2.68 (m, IH) , 2.33 (s, 3H) , 1.64 (m, 2H) , 1.39 (m, IH) , 0.93 (d, J=6 Hz, 3H) , 0.92 (d, J=6 Hz, 3H) .

12B. Formula 15 Where R ² Is 4-Methoxyphenylmethyl

By following the procedures described in Example 12A and substituting 4-methyl-2- methylenepentanoic ac. I with 3- (4-methoxyphenyl) -2- methylenepropanoic acid, 6.4 g (95% yield) of AcS- (CH ₂ -.DL- TyrOMe) -OH as a gum was obtained. Characteristic analytical data are as follows: ^α H NMR (300 MHz, CDC1 ₃ ) δ 7.08 (d, J=9 Hz, 2H) , 6.80 (d, J=9 Hz, 2H) , 3.76 (s, 3H) , 2.77-3.12 (m, 5H) , 2.29 (s, 3H) .

12C. Formula 15 Varying R ²

By following the procedures described in Example 12A and substituting 4-methyl-2- methylenepentanoic acid with other compounds of Formula 13 (where the R ² substituents are as indicated in the table below) there are obtained the corresponding substituted compounds of Formula 15.

R Name

H AcS- (CH ₂ - L-Gly) -OH methyl AcS- (CH ₂ -nL-Ala) -OH

2-propyl AcS-(CH ₂ -DL-Val) -OH

2-butyl AcS- (CH ₂ -DL-Ile) -OH benzyl AcS- (CH - L-Phe) -OH blocked 4- AcS- (CH ₂ -DL-Tyr) -OH hydroxyphenylmethyl blocked 3- AcS- (CH ₂ -DL-Trp) -OH indolylmethyl phenylethyl AcS- (CH ₂ -DL-Phet) -OH blocked 4-aminobutyl AcS- (CH ₂ -DL-Lys) -OH blocked 3- AcS- (CH ₂ -DL-Arg) -OH guanylpropyl blocked 4- AcS- (CH ₂ -DL-His) -OH imidazoylmethyl

EXAMPLE 13 PREPARATION OF HS-(CH ₂ --DL-Leu)-OH 13A. Formula 16 Where R ² is 2-Methylpropyl

A solution of 2.0 g of AcS- (CH ₂ -£>L-Leu) -OH in 5 mL of cone. NH ₄ OH was stirred at 25°C for lh. The reaction mixture was acidified to pH 4-5 by addition of 1 M HC1, and extracted with CH ₂ C1 ₂ . The organic layer was dried over Na ₂ S0 ₄ and evaporated to give 1.35 g (85% yield) of HS- (CH ₂ -IL-Leu) -OH. Characteristic analytical data are as follows: ^X H NMR (300 MHz, CDC1 ₃ ) δ 2.6-2.8 (m, 3H) , 1.64 (m, 2H) , 1.55 (t, J=7 Hz, IH, SH) , 1.44 (m, IH) , 0.95 (d, J=7 Hz, 3H) , 0.92 (d, J=7 Hz, 3H) .

13B. Formula 16 Where R ² Is 4-Methoxyphenylmethyl

By following the procedures described in Example 13A and svbstituting AcS- (CH ₂ -DL-Leu) -OH with AcS- (CH ₂ -DL-TyrOCH ₃ )-OH, 2.2 g (9.7 mmol) of HS- (CH ₂ -Iλ -Tyr OMe)-OH was obtained.

13C. Formula 16 Varying R ²

By following the procedures described in Example 13A and substituting AcS- (CH ₂ -DL-Leu) -OH with other compounds of Formula 15 (where the R ² substituents are as indicated in the table below) there are obtained the corresponding substituted compounds of Formula 16.

Formula 16

R ² Name

H HS- (CH ₂ -DL-Gly) -OH methyl HS-(CH ₂ -DL-A:a) -OH

2-propyl HS-(CH ₂ - L-Val) -OH

2-butyl HS-(CH ₂ -.DL-Ile)-OH benzyl HS- (CH ₂ -DL-Phe) -OH blocked 4- HS- (CH ₂ - L-Tyr) -OH hydroxyphenylmethyl blocked 3-indolylmethyl HS- (CH ₂ -DL-Trp) -OH phenylethyl HS- (CH ₂ -DL-Phet) -OH blocked 4-aminobutyl HS- (CH ₂ - L-Lys) -OH blocked 3-guanylpropyl HS- (CH ₂ -DL-Arg) -OH blocked 4- HS- (CH ₂ -DL-His) -OH imidazoylmethyl

EXAMPLE 14

PREPARATION OF MeS-(CH ₂ --DL-Leu) -OH

14A. Formula 17 Where R ¹ Is Methyl and R ² Is 2-Methylpropyl To a solution of 240 mg of Na in 8 mL of MeOH, was added 672 mg of HS- (CH ₂ - L-Leu) -OH and 0.322 mL of

CH ₃ I. The mixture was stirred at 25°C for 8 h. Water was added and the mixture was acidified with acetic acid and extracted with EtOAc. The organic layer was dried and evaporated to give 639 mg (88% yield) of MeS- (CH ₂ -DL-Leu) - OH. Characteristic analytical data are as follows: ^X H NMR (300 MHz, CDC1 ₃ ) δ 2.73 (m, 2H) , 2.59 (m, IH) , 2.12 (s, 3H) , 1.63 (m, 2H) , 1.43 (m, IH) , 0.94 (d, J=6 Hz, 3H) , 0.92 (d, J=6 Hz, 3H) .

14B. Formula 17 Where R ¹ Is n-butyl and R ² Is 2-Methylpropyl

By following the procedures described in

Example 14A and substituting CH ₃ I with -n-butyl iodide, 1.78 g (95% yield) of n-BuS- (CH ₂ - L-Leu) -OH was obtained. Characteristic analytical data are as follows: ^: H NMR (300 MHz, CDC1 ₃ ) δ 2.57-2.77 (m, 3H) , 2.54 (t, J=7 Hz, 2H) , 1.52-1.72 (m, 4H) , 1.34-1.48 (m, 3H) , 0.94 (d, J=6 Hz, 3H) , 0.92 (d, J=6 Hz, 3H) , 0.91 (t, J=7 Hz, 3H) .

14C. Formula 17 Where R ¹ is n-Butyl and R ² Is 4-Methoxyphenylmethyl

By following the procedures described in

Example 14A and substituting HS- (CH ₂ - L-Leu) -OH with HS- (CH ₂ - L-TyrOCH ₃ )-OH, and CH ₃ I with n-butyl iodide, 2.50 g (90% yield) of -n-BuS- (CH ₂ -DL-TyrOMe) -OH was obtained. Characteristic analytical data are as follows: ^: H NMR (300 MHz, CDCI ₃ ) δ 7.10 (d, J=9 Hz, 2H) , 6.82 (d, J=9 Hz, 2H) , 3.78 (s, 3H) , 2.72-3.02 (m, 4H) , 2.62 (dd, J=5, 13 Hz, IH), 2.48 (t, J=7 Hz, 2H) , 1.50 (m, 2H) , 1.38 (m, 2H) , 0.88 (t, J=7 Hz, 3H) .

14D. Formula 17 Varying R ¹ and R ²

By following the procedures described in Example 14A and substituting CH ₃ I with other compounds of Formula 9 (e.g., compounds with R ¹ ) and HS- (CH ₂ - L-Leu) -OH with other compounds of Formula 16 (e.g., compounds with R ² that are prepared according to Example 13A and exemplified in Example 13B) there are obtained the correspondingly substituted of compounds of Formula 17.

Formula 17

R ¹ R ² Name ethyl H EtS- (CH ₂ -jDL-Gly) -OH benzyl methyl BnS- (CH ₂ -DL-Ala) -OH phenethyl 2-propyl PhetS- (CH ₂ -DL-Val) -OH blocked 2-butyl (4-hydroxyphenethyl) -

4-hydroxyphen- S-(CH ₂ -DL-Ile)-OH ethyl blocked 2- (4- benzyl 2 (4-imidazolyl) ethyl- imidazolyl) - S-(CH ₂ -DL-Phe)-OH ethyl blocked blocked 4- (3-carboxypropyl) S- 3-carboxy¬ hydroxypheny1- (CH ₂ -DL-Tyr) -OH propyl methyl ethyl 4-methoxy¬ EtS- (CH ₂ -DL-TyrOCH ₃ ) - phenylmethyl OH benzyl blocked 3- BnS- (CH ₂ -DL-Trp) -OH indolylmethyl phenethyl phenylethyl PhetS- (CH ₂ -DL-Phet) - OH blocked blocked 4- (4-hydroxyphenethy1) -

4-hydroxyphen- aminobutyl S-(CH ₂ - L-Lys) -OH ethyl

Formula 17

R ¹ R ² Name blocked 2- (4- blocked 3- 2-(4- imidazolyl) - guanylpropyl imidazolyl) ethylS- ethyl (CH ₂ -DL-Arg)-OH blocked blocked 4- 3-carboxypropylS- 3-carboxy¬ imidazoyl- (CH ₂ -LL-His)-OH propyl ethyl ethyl H EtS- (CH ₂ - L-Gly) -OH benzyl methyl BnS- (CH ₂ -DL-Ala) -OH phenethyl 2-propyl PhetS- (CH ₂ -.DL-Val) -OH blocked 2-butyl 4-hydroxyphenethylS- -hydroxyphen- (CH ₂ -DL-Ile) -OH ethy] blocked 2- (4- benzyl 2-(4- imidazolyl) - imidazolyl) ethylS- ethyl (CH ₂ -DL-Phe) -OH blocked blocked 4- 3-carboxypropylS- 3-carboxy¬ hydroxypheny1- (CH ₂ - L-Tyr)-OH propyl methyl ethyl blocked 3- EtS- (CH ₂ -DL-Trp) -OH indolylmethyl benzyl phenylethyl BnS- (CH ₂ - L-Phet) -OH phenethyl blocked 4- PhetS- (CH ₂ -DL-Lys) -OH aminobutyl blocked blocked 3- (4-hydroxyphenethyl) - -hydroxyphen- guanylpropyl S-(CH ₂ - L-Arg) -OH ethyl blocked 2- (4- blocked 4- 2-(4- imidazolyl) - i idazoyl- imidazolyl) ethylS- ethyl ethyl (CH ₂ - L-His)-OH blocked H 3-carboxypropylS- 3-carboxy¬ (CH ₂ -DL-Gly)-OH propyl

Formula 17

R ¹ R ² Name ethyl methyl EtS- (CH ₂ -DL-Ala) -OH benzyl 2-propyl BnS- (CH ₂ -DL-Val) -OH phenethyl 2-butyl PhetS- (CH ₂ -DL-Ile) -OH blocked benzyl 4-hydroxyphenethy1S- -hydroxyphen- (CH ₂ -DL-Phe) -OH ethyl blocked 2- (4- blocked 4- 2- (4-imidazolyl) - imidazolyl) - hydroxypheny1- ethyls- (CH ₂ -£>L-Tyr) - ethyl methyl OH blocked blocked 3- 3-carboxypropylS- 3-carboxy¬ indolylmethyl (CH ₂ -DL-Trp)-OH propyl ethyl phenylethyl EtS- (CH ₂ - L-Phet) -OH benzyl blocked 4- BnS- (CH ₂ -DL-Lys) -OH aminobutyl phenethyl blocked 3- PhetS- (CH ₂ -DL-Arg) -OH guanylpropyl blocked blocked 4- 4-hydroxyphenethy1S- -hydroxyphen- imidazoyl- (CH ₂ -DL-His) -OH ethyl methyl blocked 2- (4- H 2- (4-imidazolyl) - imidazolyl) - ethylS- (CH ₂ -DL-Gly) - ethyl OH blocked methyl 3-carboxypropylS- 3-carboxy¬ (CH ₂ - L-Ala) -OH propyl ethyl 2-propyl EtS- (CH ₂ -DL-Val) -OH benzyl 2-butyl BnS- (CH ₂ -DL-Ile) -OH phenethyl benzyl PhetS- (CH ₂ -DL-Phe) -OH

Formula 17

R ¹ R ² Name blocked blocked 4- 4-hydroxyphenethylS-

4-hydroxyphen- hydroxypheny1- (CH ₂ -Iλ -Tyr) -OH ethyl methyl blocked 2- (4- blocked 3- 2- (4-imidazolyl) - imidazolyl) - indolylmethyl ethylS- (CH ₂ -DL-Trp) - ethyl OH blocked phenylethyl 3-carboxypropylS- 3-carboxy¬ (CH ₂ -DL-Phet) -OH propyl

EXAMPLE 15

PREPARATION OF MeS-(CH ₂ -.D-L-Leu-CH ₂ )-OH

15A. Formula 18 Where R ¹ Is Methyl and R ² Is 2-Methylpropyl

To a solution of 639 mg of MeS- (CH ₂ -DL-Leu) -OH in 10 mL of THF at 0°C, was added 10 mL of 1 M BH ₃ in THF dropwise over a period of 15 min with stirring. After completion of the addition, the stirring was continued for another 15 min at the same temperature. The mixture was allowed to warm to room temperature and was stirred for 3 h. The reaction mixture was cooled in an ice bath and was quenched by dropwise addition of water. The mixture was partitioned between water and EtOAc. The organic layer was washed with saturated NaHC0 ₃ and dried over Na ₂ S0 ₄ . The solvent was removed by evaporation and the residue was purified by flash chromatography (5%

EtOAc in hexane) to give 418 mg (71% yield) of MeS-(CH ₂ -

DL-Leu-CH ₂ ) -OH. Characteristic analytical data are as follows: ^X H NMR (300 MHz, CDC1 ₃ ) δ 3.70 (dd, J=4, 11 Hz,

IH) , 3.60 (dd, J=6, 11 Hz, IH) , 2.57 (dd, J=5, 13 Hz,

IH ) , 2 . 53 ( dd , J=7 , 13 Hz , IH ) , 2 . 12 ( s , 3H ) , 1 . 87 (m, 2H ) , 1 . 63 (m, IH) , 1 . 21 (m, 2H) , 0 . 89 ( d, J=7 Hz , 3H) , 0 . 88 ( d, J=7 Hz , 3H ) .

15B. Formula 18 Where R ¹ Is n-butyl and R ² Is 2-Methylpropyl

By following the procedures described in

Example 15A and substituting MeS- (CH ₂ -DL-Leu) -OH with n-BuS- (CH ₂ -DL-Leu) -OH, 1.76 g (81% yield) of n-BuS-(CH ₂ - £>L-Leu-CH ₂ ) -OH was obtained as an oil. characteristic analytical data are as follows: ^X H NMR (300 MHz, CDC1 ₃ ) δ

3.71 (dd, J=4, 11 Hz, IH) , 3.61 (dd, J=7, 11 Hz, IH) ,

2.63 (dd, J=5, 14 Hz, IH) , 2.55 (dd, J=7, 14 Hz, IH) ,

2.54 (t, J=7 Hz, 2H) , 1.85 (m, 2H) , 1.72-1.53 (m, 3H) , 1.41 (m, 2H) , 1.21 (t, J=7 Hz, 2H) , 0.92 (t, J=7 Hz, 3H) , 0.91 (d, J=7 Hz, 3H) , 0.90 (d, J=7 Hz, 3H) .

15C. Formula 18 Where R ¹ Is n-butyl and R ² Is 4-Methoxyphenylmethyl By following the procedures described in

Example 15A and substituting MeS- (CH ₂ -DL-Leu) -OH with n-BuS- (CH ₂ -DL-TyrOMe) -OH, 1.65 g (69% yield) of n-BuS- (CH ₂ -DL-TyrOMe-CH ₂ ) -OH was obtained as an oil. Characteristic analytical data are as follows: ^: H NMR (300 MHz, CDC1 ₃ ) δ 7.1 (d, J=9 Hz, 2H) , 6.83 (d, J=9 Hz, 2H) , 3.78 (s, 3H) , 3.5-3.7 (m, 2H) , 2.64 (d, J=7 Hz, 2H) ,

2.55 (d, J=7 Hz, 2H) , 2.47 (t, J=8 Hz, 2H) , 2.02 (m, 2H) , 1.52 (m, 2H) , 1.39 (m, 2H) , 0.89 (t, J=7 Hz, 3H) .

15D. Formula 18 Varying R ¹ , R ² and R ³

By following the procedures described in Example 15A and substituting MeS- (CH ₂ -DL-Leu) -OH with other compounds of Formula 17 (where R ¹ , R ² and R ³ are as indicated in the table below) there are obtained the corresponding substituted compounds of Formula 18.

Formula 18

R ¹ R ² Name ethyl H EtS-(CH ₂ -DL-Gly- CH ₂ )-OH benzyl methyl BnS-(CH ₂ - L-Ala- CH ₂ ) -OH phenethyl 2-propyl PhetS- (CH ₂ - L-Val- CH ₂ ) -OH blocked 2-butyl (4-hydroxy-

4-hydroxy- phenethyDS- (CH ₂ - phenethyl DL-Ile-CH ₂ )-OH blocked 2- (4- benzyl 2(4- imidazolyl) - imidazolyl) ethylS- ethyl (CH ₂ -DL-Phe-CH ₂ ) -OH blocked blocked 4- (3-carboxypropyl) S- 3-carboxy¬ hydroxypheny1- (CH ₂ - L-Tyr-CH ₂ ) -OH propyl methyl ethyl 4-methoxy¬ EtS- (CH ₂ -.DL-TyrOCH ₃ - phenylmethyl CH ₂ ) -OH benzyl blocked 3- BnS-(CH ₂ -DL-Trp- indolylmethyl CH ₂ )-OH phenethyl phenylethyl PhetS- (CH ₂ --DL-Phet- CH ₂ )-OH blocked blocked 4- (4-hydroxy-

4-hydroxy- aminobutyl phenethyl) -S- (CH ₂ - phenethyl L-Lys-CH ₂ ) -OH blocked 2- (4- blocked 3- 2-(4- imidazolyl) - guanylpropyl imidazolyl) ethylS- ethyl (CH ₂ -DL-Arg-CH ₂ ) -OH blocked blocked 4- 3-carboxypropylS- 3-carboxy¬ imidazoylmethyl (CH ₂ -DL-His-CH ₂ ) -OH propyl ethyl H EtS-(CH ₂ -DL-Gly- CH ₂ ) -OH

Formula 18

R ¹ R ² Name benzyl methyl BnS-(CH ₂ -nL-Ala- CH ₂ ) -OH phenethyl 2-propyl PhetS- (CH ₂ -DL-Val- CH ₂ ) -OH blocked 2-butyl 4-hydroxyphenethy1- 4-hydroxy- S-(CH ₂ - L-Ile-CH ₂ )- phenethyl OH blocked 2- (4- benzyl 2-(4- imidazolyl) - imidazolyl) ethylS- ethyl (CH ₂ -DL-Phe-CH ₂ ) -OH blocked blocked 4- 3-carboxypropylS- 3-carboxy¬ hydroxypheny1- (CH ₂ -I.L-Tyr-CH ₂ ) -OH propyl methyl ethyl blocked 3- EtS-(CH ₂ -JDL-Trp- indolylmethyl CH ₂ ) -OH benzyl phenylethyl BnS-(CH ₂ -DL-Phet- CH ₂ )-OH phenethyl blocked 4- PhetS- (CH ₂ - L-Lys- aminobutyl CH ₂ ) -OH blocked blocked 3- (4-hydroxy- 4-hydroxy¬ guanylpropyl phenethyl) S-(CH ₂ - phenethyl L-Arg-CH ₂ ) -OH blocked 2-(4- blc ed 4- 2-(4- imidazolyl) - imidaz-ylmethyl imidazolyl) ethylS- ethyl (CH ₂ -DL-His-CH ₂ ) -OH blocked H 3-carboxypropylS- 3-carboxy¬ (CH ₂ -DL-Gly-CH ₂ ) -OH propyl ethyl methyl EtS-(CH ₂ -DL-Ala- CH ₂ )-OH benzyl 2-propyl BnS-(CH ₂ -DL-Val- CH ₂ ) -OH

Formula 18

R ¹ R ² Name phenethyl 2-butyl PhetS- (CH ₂ -D -Ile- CH ₂ ) -OH blocked benzyl 4-hydroxyphenethy1- 4-hydroxy- S- (CH ₂ -DL-Phe-CH ₂ ) - phenethyl OH blocked 2- (4- blocked 4- 2- (4-imidazolyl) - imidazolyl) - hydroxypheny1- ethyls- (CH ₂ -DL-Tyr- ethyl methyl CH ₂ ) -OH blocked blocked 3- 3-carboxypropylS- 3-carboxy¬ indolylmethyl (CH ₂ - L-Trp-CH ₂ ) -OH propyl ethyl phenylethyl EtS-(CH ₂ -DL-Phet- CH ₂ ) -OH benzyl blocked 4- BnS-(CH ₂ -DL-Lys- aminobutyl CH ₂ )-OH phenethyl blocked 3- PhetS- (CH ₂ -£ ⁾ L-Arg- guanylpropyl CH ₂ ) -OH blocked blocked 4- 4-hydroxyphen- 4-hydroxy- imidazoylmethyl ethylS- (CH ₂ - L-His- phenethyl CH ₂ ) -OH blocked 2- (4- H 2- (4-imidazolyl) - imidazolyl) - ethyls- (CH ₂ -DL-Gly- ethyl CH ₂ )-OH blocked methyl 3-carboxypropylS- 3-carboxy¬ (CH ₂ - L-Ala-CH ₂ ) -OH propyl ethyl 2-propyl EtS-(CH ₂ -PL-Val- CH ₂ )-OH benzyl 2-butyl BnS-(CH ₂ -DL-Ile- CH ₂ ) -OH phenethyl benzyl PhetS- (CH ₂ -DL-Phe- CH ₂ ) -OH

Formula 18

R ¹ R ² Name blocked blocked 4- 4-hydroxyphen- 4-hydroxy- hydroxypheny1- ethylS- (CH ₂ - L-Tyr- phenethyl methyl CH ₂ ) -OH blocked 2- (4- blocked 3- 2- (4-imidazolyl) - imidazolyl) - indolylmethyl ethyls- (CH ₂ -DL-Trp- ethyl CH ₂ ) -OH blocked phenylethyl 3-carboxypropylS- 3-carboxy¬ (CH ₂ -DL-Phet-CH ₂ ) -OH propyl

EXAMPLE 16

PREPARATION OF MeS-(CH ₂ --DL-Leu-CH ₂ ) -OTBS

16A. Formula 19 Where R ¹ Is Methyl and R ² Is 2-Me lpropyl

'.. a solution of 418 mg of MeS- (CH ₂ -DL-Leu-

CH ₂ )-OH in 2 mL of DMF, was added 431 mg of imidazole and

451 mg of t-butyldimethylsilyl chloride. The mixture was stirred at room temperature for 7 h. The mixture was partitioned between Et ₂ 0 and water and the organic layer was washed with water, dried, and evaporated under vacuum. The residue was purified by flash chromatography on silica gel (5% EtOAc in hexane) to give 570 mg (80% yield) of MeS- (CH ₂ - L-Leu-CH ₂ ) -OTBS as an oil.

Characteristic analytical data are as follows: H NMR

(300 MHz, CDC1 ₃ ) δ 3.61 (dd, J=5, 10 Hz, IH) , 3.52 (dd,

J=6, 10 Hz, IH) , 2.59 (dd, J=7, 13 Hz, IH) , 2.43 (dd,

J=6, 13 Hz, IH) , 2.07 (s, 3H) , 1.74 (m, IH) , 1.64 (m,

IH) , 1.20 ( , 2H) , 0.89-0.85 (m, 15H) , 0.06 (s, 6H) .

16B. Formula 19 Where R ¹ Is n-butyl and R ² Is 2-Methylpropyl

By following the procedures described in

Example 16A and substituting MeS- (CH ₂ -DL-Leu-CH ₂ ) -OH with n-BuS-(CH ₂ -.DL-Leu-CH ₂ ) -OH, 2.16 g (79% yield) of n-BuS-

(CH ₂ --DL-Leu-CH ₂ ) -OTBS was obtained as an oil.

Characteristic analytical data are as follows: ^X H NMR

(300 MHz, CDC1 ₃ ) δ 3.62 (dd, J=4, 10 Hz, IH) , 3.52 (dd,

J=6, 10 Hz, IH) , 2.62 (dd, J=7, 13 Hz, IH) , 2.40-2.52 (m, 3H) , 1.51-1.78 (m, 4H) , 1.41 (m, 2H) , 1.21 (dt, J=2,

7 Hz, 2H) , 0.91 (t, J=7 Hz, 3H) , 0.89 (s, 9H) , 0.89 (d,

J=7 Hz, 3H) , 0.88 (d, J=7 Hz, 3H) , 0.04 (s, 6H) .

16C. Formula 19 Where R ¹ Is n-butyl and R ² Is 4-Methoxyphenylmethyl

By following the procedures described in

Example 16A and substituting MeS- (CH ₂ -DL-Leu-CH ₂ ) -OH with

MeS-(CH ₂ - L-TyrOCH ₃ -CH ₂ )-OH, 1.94 g (85% yield) of n-BuS-

(CH ₂ - L-TyrOMe-CH ₂ ) -OTBS was obtained as an oil. Characteristic analytical data are as follows: H NMR

(300 MHz, CDC1 ₃ ) δ 7.10 (d, J=9 Hz, 2H) , 6.82 (d, J=9 Hz, 2H) , 3.78 (s, 3H) , 3.58 (dd, J=5, 10 Hz, IH) , 3.51 (dd, J=5, 10 Hz, IH) , 2.62 (dd, J=4, 7 Hz, 2H) , 2.55 (dd, J=7, 13 Hz, IH) , 2.44.m (3), 1.84 (m, IH) , 1.50 (m, 2H) , 1.38 (m, 2H) , 0.89 (s, 9H) , 0.88 (t, J=7 Hz, 3H) , 0.02 (s, 6H) .

16D. Formula 19 Varying R ¹ , R ² and R ³

By following the procedures described in Example 16A and substituting MeS- (CH ₂ -L>L-Leu-CH ₂ ) -OH with other compounds of Formula 18 (where R ¹ , R ² and R ³ are as indicated in the table below) there are obtained the corresponding substituted compounds of Formula 19.

Formula 19

G1 ¹ )

R ¹ R ² Name ethyl H EtS- (CH ₂ -DL-Gly-CH ₂ ) - OTBS benzyl methyl BnS- (CH ₂ -DL-Ala-CH ₂ ) - OTBS phenethyl 2-propyl PhetS- (CH ₂ -DL-Val- CH ₂ )-OTBS blocked 2-butyl (4-hydroxy- 4-hydroxy- phenethyl) S- (C -DL- phenethyl Ile-CH ₂ )-O ^r - 3 blocked 2- (4- benzyl 2(4- imidazolyl) - imidazolyl) ethylS- ethyl (CH ₂ -DL-Phe-CH ₂ ) -OTBS blocked blocked 4- (3-carboxypropyl) S- 3-carboxy¬ hydroxypheny1- (CH ₂ - L-Tyr-CH ₂ ) -OTBS propyl methyl ethyl 4-methoxy¬ EtS- (CH ₂ -DL-TyrOCH ₃ - phenylmethyl CH ₂ )-OTBS benzyl blocked 3- BnS- (CH ₂ -DL-Trp-CH ₂ ) - indolylmethyl OTBS phenethyl phenylethyl PhetS- (CH ₂ -DL-Phet- CH ₂ )-OTBS blocked blocked ' - (4-hydroxy¬

4-hydrox - aminobu" ^* , 1 phenethy1) -S- (CH ₂ - phenethyl DL-Lys-CH ₂ )-OTBS blocked 2- (4- blocked 3- 2- (4-imidazolyl) - imidazolyl) - guanylpropyl ethylS- (CH ₂ -DL-Arg- ethyl CH ₂ )-OTBS blocked blocked 4- 3-carboxypropylS- 3-carboxy¬ imidazoylmethyl (CH ₂ -jDL-His-CH ₂ ) -OTBS propyl ethyl H EtS- (CH ₂ -DL-Gly-CH ₂ ) - OTBS

Formula 19

R ¹ R ² Name benzyl methyl BnS- (CH ₂ -DL-Ala-CH ₂ ) - OTBS phenethyl 2-propyl PhetS- (CH ₂ -DL-Val- CH ₂ ) -OTBS blocked 2-butyl 4-hydroxyphenethy1S- 4-hydroxy- (CH ₂ - L-Ile-CH ₂ ) -OTBS phenethyl blocked 2- (4- benzyl 2-(4- imidazolyl) - imidazolyl) ethylS- ethyl (CH ₂ - L-Phe-CH ₂ ) -OTBS blocked blocked 4- 3-carboxypropylS- 3-carboxy¬ hydroxypheny1- (CH ₂ - L-Tyr-CH ₂ ) -OTBS propyl methyl ethyl blocked 3- EtS- (CH ₂ -DL-Trp-CH ₂ ) - indolylmethyl OTBS benzyl phenylethyl BnS- (CH ₂ -DL-Phet- CH ₂ ) -OTBS phenethyl blocked 4- PhetS- (CH ₂ -DL-Lys- aminobutyl CH ₂ ) -OTBS blocked blocked 3- (4-hydroxy- 4-hydroxy- guanylpropyl phenethyl) S- (CH ₂ --D - phenethyl Arg-CH ₂ ) -OTBS blocked 2- (4- blocked 4- 2- (4-imidazolyl) - imidazolyl) - i idazoylmethyl ethylS- (CH ₂ -L>L-His- ethyl CH ₂ )-OTBS blocked H 3-carboxypropylS- 3-carboxy¬ (CH ₂ -DL-Gly-CH ₂ ) -OTBS propyl ethyl methyl EtS- (CH ₂ -DL-Ala-CH ₂ ) - OTBS benzyl 2-propyl BnS- (CH ₂ -DL-Val-CH ₂ ) - OTBS

Formula 19

R ¹ R ² Name phenethyl 2-butyl PhetS- (CH ₂ -DL-Ile- CH ₂ )-OTBS blocked benzyl 4-hydroxyphenethy1S- 4-hydroxy- (CH ₂ - L-Phe-CH ₂ ) -OTBS phenethyl blocked 2- (4- blocked 4- 2- (4-imidazolyl) - imidazolyl) - hydroxypheny1- ethylS- (CH ₂ -DL-Tyr- ethyl methyl CH ₂ )-OTBS blocked blocked 3- 3-carboxypropylS- 3-carboxy¬ indolylmethyl (CH ₂ -DL-Trp-CH ₂ ) -CTBS propyl ethyl phenylethyl EtS-(CH ₂ - L-Phet- CH ₂ ) -OTBS benzyl blocked 4- BnS- (CH ₂ - L-Lys-CH ₂ ) - aminobutyl OTBS phenethyl blocked 3- PhetS- (CH ₂ - L-Arg- guanylpropyl CH ₂ )-OTBS blocked blocked 4- 4-hydroxyphenethy1S- 4-hydroxy- imidazoylmethy1 (CH ₂ - L-His-CH ₂ ) -OTBS phenethyl blocked 2- (4- H 2- (4-imidazolyl) - imidazolyl) - ethyls- (CH ₂ -DL-Gly- ethyl CH ₂ ) -OTBS blocked methyl 3-carboxypropylS- 3-carboxy¬ (CH ₂ - L-Ala-CH ₂ ) -OTBS propyl ethyl 2-propyl EtS- (CH ₂ -DL-Val-CH ₂ ) - OTBS benzyl 2-butyl BnS- (CH ₂ -DL-Ile-CH ₂ ) - OTBS phenethyl benzyl PhetS- (CH ₂ -DL-Phe- CH ₂ )-OTBS

Formula 19

R ¹ R ² Name blocked blocked 4- 4-hydroxyphenethy1S-

4-hydroxy- hydroxypheny1- (CH ₂ - L-Tyr-CH ₂ ) -OTBS phenethyl methyl blocked 2- (4- blocked 3- 2- (4-imidazolyl) - imidazolyl) - indolylmethyl ethylS- (CH ₂ -DL-Trp- ethyl CH ₂ ) -OTBS blocked phenylethyl 3-carboxypropylS- 3-carboxy¬ (CH ₂ -.DL-Phet-CH ₂ ) - propyl OTBS

EXAMPLE 17

PREPARATION OF MeS(NH) ₂ -(CH ₂ -DL-Leu-CH ₂ ) -OTBS

17A. Formula 20 Where X Is NH, R ¹ Is Methyl and R ² Is 2-Methylpropyl

The sulfodiimine functionality was introduced using a modification of Mock's procedure [see Mock, .

L.; Tsay, J. T. J. Am. Chem. Soc . 1989, 111 , 4467-4472] .

To a stirred solution of 552 mg of MeS- (CH ₂ -DL-Leu-CH ₂ ) -

OTBS in 2 mL of anhydrous acetonitrile and 2 mL of anhydrous liquid ammonia (distilled from Na) at -55°C under nitrogen was added a solution of 668 mg of N- chlorosuccinimide in 3 mL of acetonitrile dropwise over a period of 5 min. The reaction mixture was stirred at -

55°C for 30 min, then it was warmed to room temperature and stirring was continued overnight. The solvent was evaporated under vacuum and the residue was partitioned between 10% aqueous NaOH and CH ₂ C1 ₂ . The organic layer was dried over Na ₂ S0 ₄ and evaporated under vacuum. The residue was purified by flash chromatography (5% MeOH in

CH ₂ C1 ₂ ) to afford 95 mg (17% yield) of unreacted starting

material and 273 mg (54% yield based on unrecovered starting material) of the sulfodiimine MeS (NH) ₂ - (CH ₂ - L- Leu-CH ₂ ) -OTBS as a gum. Characteristic analytical data are as follows: ^X H NMR (300 MHz, CDC1 ₃ ) δ 3.77 (dd, J=4, 10 Hz, IH) , 3.55 (dd, J=5, 10 Hz, IH) , 3.37 (dd, J=6, 14 Hz, IH) , 3.00 (s, 3H) , 2.90 (dd, J=5, 14 Hz, IH) , 2.48 (m, IH) , 1.62 (m, IH) , 1.21-1.44 (m, 2H) , 0.91 (d, J=7 Hz, 3H) , 0.90 (d, J=7 Hz, 3H) , 0.87 (s, 9H) , 0.03 (s, 6H) ; mass spectrum (PCI), m/e 307 (MH ⁺ , 100) .

17B. Formula 20 Where X Is NH, R ¹ Is n-butyl and R ² Is 2-Methylpropyl

By following the procedures described in

Example 17A and substituting MeS- (CH ₂ -IλL-Leu-CH ₂ ) -OTBS with n-BuS-(CH ₂ -DL-Leu-CH ₂ )-OTBS, 227 mg (45% yield, based on recovered starting material) of n-BuS (NH) ₂ - (CH ₂ - L-Leu-

CH ₂ )-OTBS was obtained as a gum. Characteristic analytical data are as follows: ^X E NMR (300 MHz, CDC1 ₃ ) δ

3.78 (dd, J=4, 10 Hz, iH) , 3.58 (dd, J=5, 10 Hz, IH) , 3.31 (dd, J=6, 14 Hz, IH) , 3.03 (m, 2H) , 2.84 (dd, J=5,

14 Hz, IH) , 2.54 (m, IH) , 1.6-1.8 (m, 4H) , 1.26-1.54 (m, 3H) , 0.97 (t, J=7 Hz, 3H) , 0.94 (d, J=6 Hz, 3H) , 0.92 (d, J=6 Hz, 3H) , 0.89 (s, 9H) , 0.06 (s, 6H) ; mass spectrum (PCI), /e 349 (MH ⁺ , 100), 332 (21), 291 (53), 235 (29), 171 (20) , 121 (16) .

17C. Formula 20 Where X Is NH, R ¹ Is n-butyl and R ² Is 4-Methoxyphen lmethyl

By following the procedures described in Example 17A and substituting MeS- (CH ₂ --DL-Leu-CH ₂ ) -OTBS with n-BuS-(CH ₂ -DL-TyrOCH ₃ -CH ₂ ) -OTBS, 250 mg (53% yield, based on recovered starting material) of the sulfodiimine

-n-BuS(NH) ₂ - (CH ₂ - >L-TyrOMe-CH ₂ ) -OTBS was obtained as a gum.

Characteristic analytical data are as follow: H NMR (300 MHz, CDC1 ₃ ) δ 7.12 (d, J=9 Hz, 2H) , 6.84 (d, J=9 Hz, 2H) ,

3.79 (s, 3H) , 3.72 (dd, J=5, 10 Hz, IH) , 3.60 (dd, J=5,

10 Hz, IH) , 3.26 (dd, J=7 , 14 Hz, IH) , 3.04 (dd, J=5, 14 Hz, IH) , 2.91 (t, J=8 Hz, 2H) , 2.78 (dd, J=8, 14 Hz, IH) , 2.71 (dd, J=7, 14 Hz, IH) , 2.42 (m, IH) , 1.71 (m, IH) , 1.62 (m, IH) , 1.38 (m, 2H) , 0.92 (t, J=7 Hz, 3H) , 0.90 (s, 9H) , 0.03 (s, 6H) .

17D. Formula 20 Varying R ¹ , R ² and R ³

By following the procedures described in Example 17A and substituting MeS- (CH ₂ - L-Leu-CH ₂ ) -OTBS with other compounds of Formula 19 (where R ¹ , R ² and R ³ are as indicated in the table below) there are obtained the corresponding substituted compounds of Formula 20.

Formula 20

R ¹ R ² Name ethyl H EtS (NH) ₂ - (CH ₂ -DL-Gly- CH ₂ ) -OTBS benzyl methyl BnS (NH) ₂ - (CH ₂ - L-Ala- CH ₂ )-OTBS phenethyl 2-propyl PhetS (NH) ₂ - (CH ₂ -DL- Val-CH ₂ ) -OTBS blocked 2-butyl (4-hydroxyphenethyl) -

4-hydroxyphen- S(NH) ₂ -(CH ₂ -DL-Ile- ethyl CH ₂ ) -OTBS blocked 2- (4- benzyl 2 (4-imidazolyl) ethyl- imidazolyl) - S(NH) ₂ -(CH ₂ - L-Phe- ethyl CH ₂ ) -OTBS blocked blocked 4- (3-carboxy¬ 3-carboxy¬ hydroxypheny1- propyl) S (NH) ₂ - (CH ₂ -DL- propyl methyl Tyr-CH ₂ ) -OTBS ethyl 4-methoxy¬ EtS(NH) ₂ -(CH ₂ -DL- phenylmethyl TyrOCH ₃ -CH ₂ )-OTBS

Formula 20

R ¹ R ² Name benzyl blocked 3- BnS (NH) ₂ - (CH ₂ - L-Trp- indolylmethyl CH ₂ )-OTBS phenethyl phenylethyl PhetS (NH) ₂ - (CH ₂ -DL- Phet-CH ₂ )-OTBS blocked blocked 4- (4-hydroxyphenethy1) - -hydroxyphen- aminobutyl S (NH) ₂ - (CH ₂ -DL-Lys- ethyl CH ₂ )-OTBS blocked 2- (4- blocked 3- 2-(4- imidazolyl) - guanylpropyl imidazolyl) ethyl- ethyl S (NH) ₂ - (CH ₂ - L-Arg-

CH ₂ )-OTBS blocked blocked 4- 3-carboxypropyl- 3-carboxy¬ imidazoylmethyl S (NH) ₂ - (CH ₂ -DL-His- propyl CH ₂ )-OTBS ethyl H EtS (NH) ₂ - (CH ₂ -DL-Gly- CH ₂ ) -OTBS benzyl methyl BnS (NH) ₂ - (CH ₂ -DL-Ala- CH ₂ ) -OTBS phenethyl 2-propyl PhetS (NH) ₂ - (CH ₂ -DL- Val-CH ₂ ) -OTBS blocked 2-butyl 4-hydroxyphenethy1- -hydroxyphen- S (NH) ₂ - (CHj-D -Ile- ethyl CH ₂ ) -OTBS blocked 2- (4- benzyl 2-(4- imidazolyl) - imidazolyl) ethyl- ethyl S (NH) ₂ - (CH ₂ -DL-Phe-

CH ₂ )-OTBS blocked blocked 4- 3-carboxypropyl- 3-carboxy¬ hydroxypheny1- S (NH) ₂ - (CH ₂ -DL-Tyr- propyl methyl CH ₂ )-OTBS ethyl blocked 3- EtS (NH) ₂ - (CH ₂ -DL-Trp- indolylmethyl CH ₂ )-OTBS

Formula 20

R ¹ R ² Name benzyl phenylethyl BnS (NH) ₂ - (CH ₂ -DL-Phet- CH ₂ )-OTBS phenethyl blocked 4- PhetS(NH) ₂ -(CH ₂ -DL- aminobutyl Lys-CH ₂ ) -OTBS blocked blocked 3- (4-hydroxyphenethy1) - -hydroxyphen- guanylpropyl S(NH) ₂ - (CH ₂ -DL-Arg- ethyl CH ₂ ) -OTBS blocked 2- (4- blocked 4- 2-(4- imidazolyl) - imidazoylmethyl imidazolyl) ethyl- ethyl S (NH) ₂ - (CH ₂ - L-His-

CH ₂ )-OTBS blocked H 3-carboxypropyl- 3-carboxy¬ S (NH) ₂ - (CH ₂ -.DL-Gly- propyl CH ₂ ) -OTBS ethyl methyl EtS (NH) ₂ - (CH ₂ -DL-Ala- CH ₂ )-OTBS benzyl 2-propyl BnS (NH) ₂ - (CH ₂ -DL-Val- CH ₂ ) -OTBS phenethyl 2-butyl PhetS (NH) ₂ -(CH ₂ -DL- Ile-CH ₂ ) -OTBS blocked benzyl 4-hydroxyphenethy1- -hydroxyphen- S (NH) ₂ - (CH ₂ -DL-Phe- ethyl CH ₂ )-OTBS blocked 2- (4- blocked 4- 2- (4-imidazolyl) - imidazolyl) - hydroxypheny1- ethylS(NH) ₂ - (CH ₂ -DL- ethyl methyl Tyr-CH ₂ )-OTBS blocked blocked 3- 3-carboxy- 3-carboxy¬ indolylmethyl propylS (NH) ₂ - (CH ₂ -DL- propyl Trp-CH ₂ )-OTBS ethyl phenylethyl EtS (NH) ₂ - (CH ₂ -DL-Phet- CH ₂ ) -OTBS benzyl blocked 4- BnS (NH) ₂ - (CH ₂ -DL-Lys- aminobutyl CH ₂ )-OTBS

Formula 20

R ¹ R ² Name phenethyl blocked 3- PhetS(NH) ₂ - (CH ₂ - L- guanylpropyl Arg-CH ₂ ) -OTBS blocked blocked 4- 4-hydroxyphen- -hydroxyphen- imidazoylmethyl ethylS(NH) ₂ - (CH ₂ -DL- ethyl His-CH ₂ )-OTBS blocked 2- (4- H 2-(4-imidazolyl) - imidazolyl) - ethylS(NH) ₂ - (CH ₂ -.DL- ethyl Gly-CH ₂ ) -OTBS blocked methyl 3-carboxy¬ 3-carboxy¬ propylS(NH) ₂ -(CH ₂ - L- propyl Ala-CH ₂ ) -OTBS ethyl 2-propyl EtS(NH) ₂ - (CH ₂ -DL-Val- CH ₂ ) -OTBS benzyl 2-butyl BnS(NH) ₂ -(CH ₂ -DL-Ile- CH ₂ )-OTBS phenethyl benzyl PhetS(NH) ₂ -(CH ₂ -Iλ - Phe-CH ₂ )-OTBS blocked blocked 4- 4-hydroxyphen-

4-hydroxyphen- hydroxypheny1- ethylS(NH) ₂ - (CH ₂ -DL- ethyl methyl Tyr-CH ₂ ) -OTBS blocked 2- (4- blocked 3- 2-(4-imidazolyl) - imidazolyl) - indolylmethyl ethylS(NH) ₂ - (CH ₂ - L- ethyl Trp-CH ₂ ) -OTBS blocked phenylethyl 3-carboxy- 3-carboxy¬ propylS(NH) ₂ - (CH ₂ -DL- propyl Phet-CH ₂ )-OTBS

EXAMPLE 18

PREPARATION OF MeS(NCbz) ₂ -(CH ₂ --DL-Leu-CH ₂ ) -OTBS

18A. Formula 21 Where R ¹ Is Methyl and R ² Is 2-Methylpropyl To a solution of 566 mg of the sulfodiimine

MeS (NH) ₂ -(CH ₂ -DL-Leu-CH ₂ ) -OTBS in 5 mL of CH ₂ C1 ₂ was added

0.76 mL of pyridine and 1 mL of benzyl chloroformate, and the mixture was stirred for 6 h at room temperature.

Saturated aqueous NaHC0 ₃ was added and the mixture was extracted with CH ₂ C1 ₂ . The organic layer was evaporated under vacuum and the residue was subjected to flash chromatography (15% EtOAc in hexane) to give 800 mg (75% yield) of MeS (NCbz) ₂ - (CH ₂ --DL-Leu-CH ₂ ) -OTBS as a gum. Characteristic analytical data are as follows: ^X H NMR (300 MHz, CDC1 ₃ ) δ 7.27-7.39 (m, 10H) , 5.06-5.16 (m, 4H) , 3.82 (dd, J=6, 14 Hz, IH) , 3.68-3.76 (m, 2H) , 3.52 (s, 3H) , 3.48 (dd, J=5, 11 Hz, IH) , 2.24 (m, IH) , 1.56 (m, IH) , 1.2-1.4 (m, 2H) , 0.88 (s, 9H) , 0.85 (d, J=7 Hz, 3H) , 0.84 (d, J=7 Hz, 3H) , 0.03 (s, 6H) .

18B. Formula 21 Where R ¹ Is n-butyl and R ² Is 2-Methylpropyl

By following the procedures described in

Example 18A and substituting MeS (NH) ₂ - (CH ₂ -DL-Leu-CH ₂ ) - • OTBS with n-BuS(NH) ₂ -(CH ₂ -DL-Leu-CH ₂ )-OTBS, 550 mg (67% yield) of n-BuS (NCbz) ₂ - (CH ₂ -L>L-Leu-CH ₂ ) -OTBS was obtained as a gum. Characteristic analytical data are as follows: H NMR (300 MHz, CDC1 ₃ ) δ 7.27-7.4 (m, 10H) , 5.13 (d, J=13 Hz, IH) , 5.11 (s, 2H) , 5.07 (d, J=13 Hz, IH) , 3.59-3.94 (m, 5H) , 3.48 (dd, J=5, 11 Hz, IH) , 2.19 (m, IH) , 1.70 (m, 2H) , 1.57 (m, IH) , 1.38 (m, 3H) , 1.23 (m, IH) , 0.89 (t, J=7 Hz, 3H) , 0.88 (s, 9H) , 0.84 (s, 3H) , 0.82 (s, 3H) , 0.03 (s, 6H) .

18C. Formula 21 Where R ¹ Is n-butyl and R ² Is 4-Methoxyphenylmethyl

By following the procedures described in

Example 18A and substituting MeS (NH) ₂ - (CH ₂ -DL-Leu-CH ₂ ) - OTBS with Ω-BuS(NH) ₂ -(CH ₂ -DL-TyrOMe-CH ₂ ) -OTBS, 685 mg (66% yield) of n-BuS (NCbz) ₂ - (CH ₂ --DL-TyrOMe-CH ₂ ) -OTBS was obtained as a gum. Further elution of the column gave 82 mg of starting material and 85 mg of the mono-Cbz sulfodiimine. Characteristic analytical data of n-BuS (NCbz) ₂ - (CH ₂ -DL-Tyr'" e-CH ₂ ) -OTBS are as follows: ^: H NMR (500 MHz, CDC1 ₃ ) δ 7.27-7.40 (m, 10H) , 7.02 (d, J=9 Hz, 2H) , 6.79 (d, J=9 Hz, 2H) , 5.12 (d, J=8 Hz, IH) , 5.09 (s, 2H) , 5.07 (d, J=8 Hz, IH) , 3.89 (dd, J=6, 15 Hz, IH) , 3.78 (s, 3H) , 3.54-3.68 (m, 4H) , 3.48 (dd, J=4, 10 Hz, IH) , 2.68 (d, J=7 Hz, 2H) , 2.34 (br m, IH) , 1.6 (m, IH) , 1.45 (m, IH) , 1.3 (m, 2H) , 0.88 (s, 9H) , 0.84 (t, J=7 Hz, 3H) , 0.03 (s, 6H) .

18C. Formula 21 Varying R ¹ , R ² and R ³ By following the procedures described in

Example 18A and substituting MeS (NH ₂ ) - (CH ₂ --DL-Leu-CH ₂ ) - OTBS with other compounds of Formula 20 (where R ¹ , R ² and R ³ are as indicated in the table below) there are obtained the corresponding substituted compounds of Formula 21.

Formula 21

DL

R ¹ R ² Name ethyl H EtS (NCbz) ₂ -(CH ₂ -DL- Gly-CH ₂ ) -OTBS benzyl methyl BnS (NCbz) ₂ -(CH ₂ - L- Ala-CH ₂ ) -OTBS phenethyl 2-propyl PhetS (NCbz) ₂ - (CH ₂ -DL- Val-CH ₂ )-OTBS blocked 2-butyl (4-hydroxyphenethy1) - 4-hydroxy- S(NCbz) ₂ - (CH ₂ - L-Ile- phenethyl CH ₂ )-OTBS blocked 2- (4- benzyl 2 (4-imidazolyl) ethyl- imidazolyl) - S (NCbz) ₂ - (CH ₂ - L-Phe- ethyl CH ₂ )-OTBS blocked blocked 4- (3-carboxy¬ 3-carboxy¬ hydroxypheny1- propyl)S (NCbz) ₂ - (CH ₂ - propyl methyl DL-Tyr-CH ₂ ) -OTBS ethyl 4-methoxy¬ EtS (NCbz) ₂ -{CH ₂ -DL- phenylmethyl TyrOCH ₃ -CH ₂ ) -OTBS benzyl blocked 3- BnS (NCbz) ₂ - (CH ₂ -DL- indolylmethyl Trp-CH ₂ ) -OTBS phenethyl phenylethyl PhetS (NCbz) ₂ -(CH ₂ -DL- Phet-CH ₂ ) -OTBS blocked blocked 4- (4-hydroxyphenethy1) - 4-hydroxy- aminobutyl S(NCbz) ₂ -(CH ₂ -.DL-Lys- phenethyl CH ₂ )-OTBS blocked 2- (4- blocked 3- 2- (4-imidazolyl) - imidazolyl) - guanylpropyl ethylS (NCbz) ₂ -(CH ₂ -DL- ethyl Arg-CH ₂ )-OTBS blocked blocked 4- 3-carboxypropyl- 3-carboxy¬ imidazoylmethyl S (NCbz) ₂ - (CH ₂ -LL-His- propyl CH ₂ ) -OTBS ethyl H EtS (NCbz) ₂ -(CH ₂ -DL- Gly-CH ₂ ) -OTBS

Formula 21

DL

R ¹ R ² Name benzyl methyl BnS (NCbz) ₂ -(CH ₂ -DL- Ala-CH ₂ ) -OTBS phenethyl 2-propyl PhetS (NCbz) ₂ - (CH ₂ - L- Val-CH ₂ )-OTBS blocked 2-butyl 4-hydroxyphenethy1-

4-hydroxy- S(NCbz) ₂ -(CH ₂ -I?L-Ile- phenethyl CH ₂ ) -OTBS blocked 2- (4- benzyl 2- (4-imidazolyl) - imidazolyl) - ethylS (NCbz) ₂ - (CH ₂ - L- ethyl Phe-CH ₂ )-OTBS blocked blocked 4- 3-carboxypropyl- 3-carboxy¬ hydroxypheny1- S (NCbz) ₂ - (CH ₂ -DL-Tyr- propyl methyl CH ₂ ) -OTBS ethyl blocked 3- EtS (NCbz) ₂ - (CH ₂ -DL- indolylmethyl Trp-CH ₂ ) -OTBS benzyl phenylethyl BnS (NCbz) ₂ - (CH ₂ -DL- Phet-CH ₂ )-OTBS phenethyl blocked 4- PhetS (NCbz) ₂ - (CH ₂ -DL- aminobutyl Lys-CH ₂ )-OTBS blocked blocked 3- (4-hydroxyphenethy1) -

4-hydroxy- guanylpropyl S(NCbz) ₂ -(CH ₂ -DL-Arg- phenethyl CH ₂ )-OTBS blocked 2- (4- blocked 4- 2-(4- imidazolyl) - imidazoylmethyl imidazolyl) ethyl- ethyl S(NCbz) ₂ -(CH ₂ -PL-His-

CH ₂ ) -OTBS blocked H 3-carboxypropyl- 3-carboxy¬ S(NCbz) ₂ -(CH ₂ -DL-Gly- propyl CH ₂ ) -OTBS ethyl methyl EtS (NCbz) ₂ -(CH ₂ -DL- Ala-CH ₂ ) -OTBS

Formula 21

DL

R ¹ R ² Name benzyl 2-propyl BnS (NCbz) ₂ - (CH ₂ -DL- Val-CH ₂ )-OTBS phenethyl 2-butyl PhetS (NCbz) ₂ -(CH ₂ -DL- Ile-CH ₂ ) -OTBS blocked benzyl 4-hydroxyphenethy1- 4-hydroxy- S (NCbz) ₂ - (CH ₂ -DL-Phe- phenethyl CH ₂ )-OTBS blocked 2- (4- blocked 4- 2- (4-imidazolyl) - imidazolyl) - hydroxypheny1- ethylS (NCbz) ₂ -(CH ₂ -JL- ethyl methyl Tyr-CH ₂ )-OTBS blocked blocked 3- 3-carboxy- 3-carboxy¬ indolylmethyl propylS(NCbz) ₂ -(CH ₂ - propyl L-Trp-CH ₂ )-OTBS ethyl phenylethyl EtS (NCbz) ₂ - (CH ₂ -DL- Phet-CH ₂ ) -OTBS benzyl blocked 4- BnS (NCbz) ₂ - (CH ₂ -DL- aminobutyl Lys-CH ₂ ) -OTBS phenethyl blocked 3- PhetS (NCbz) ₂ - (CH ₂ -IL- guanylpropyl Arg-CH ₂ )-OTBS blocked blocked 4- 4-hydroxyphen- 4-hydroxy- imidazoylmethyl ethylS (NCbz) ₂ -(CH ₂ -DL- phenethyl His-CH ₂ ) -OTBS blocked 2- (4- H 2- (4-imidazolyl) - imidazolyl) - ethylS (NCbz) ₂ -(CH ₂ -DL- ethyl Gly-CH ₂ )-OTBS blocked methyl 3-carboxy- 3-carboxy¬ propylS(NCbz) ₂ -(CH ₂ - propyl DL-Ala-CH ₂ )-OTBS ethyl 2-propyl EtS (NCbz) ₂ - (CH ₂ -DL- Val-CH ₂ )-OTBS benzyl 2-butyl BnS (NCbz) ₂ -(CH ₂ -DL- Ile-CH ₂ ) -OTBS

Formula 21

DL

R ¹ R ² Name phenethyl benzyl PhetS (NCbz) ₂ -{CH ₂ -DL- Phe-CH ₂ ) -OTBS blocked blocked 4- 4-hydroxyphen-

4-hydroxy- hydroxypheny1- ethylS(NCbz) ₂ -(CH ₂ -DL- phenethyl methyl Tyr-CH ₂ ) -OTBS blocked 2- (4- blocked 3- 2- (4-imidazolyl) - imidazolyl) - indolylmethyl ethylS (NCbz) ₂ -(CH ₂ -LL- ethyl Trp-CH ₂ ) -OTBS blocked phenylethyl 3-carboxy- 3-carboxy¬ propylS(NCbz) ₂ -(CH ₂ - propyl DL-Phet-CH ₂ ) -OTBS

EXAMPLE 19

PREPARATION OF MeS(NCbz) ₂ -(CH ₂ -DI,-Leu-CH ₂ ) -OH

19A. Formula 22 Where R ¹ Is Methyl and R ² Is 2-Methylpropyl

To a solution of 762 mg of MeS(NCbz) ₂ - (CH ₂ -LL-

Leu-CH ₂ ) -OTBS in 5 mL of THF was added 838 mg of tetrabutylammonium fluoride hydrate, and the solution was stirred at room temperature for 2.5 h. The mixture was diluted with EtOAc and was washed with water. The organic layer was dried over Na ₂ S0 ₄ and evaporated under vacuum. The residue was purified by flash chromatography (60% EtOAc in hexane) to give 444 mg (72% yield) of MeS(NCbz) ₂ -(CH ₂ -DL-Leu-CH ₂ ) -OH as a gum. Characteristic analytical data are as follows: H NMR (300 MHz, CDC1 ₃ ) δ 7.24-7.38 (mlO), 5.10 (m, 4H) , 3.85 (dd, J=8, 14 Hz, IH) , 3.76 (m, IH), 3.65 (dd, J=4, 14 Hz, IH) , 3.55 (s, 3H) ,

3 . 41 ( dd , J=6 , 11 Hz , IH ) , 2 . 22 (m, IH ) , 1 . 56 (m, IH ) , 1 . 16 -1 . 35 (m2 ) , 0 . 84 ( s , 3H ) , 0 . 82 ( s , 3H ) .

19B. Formula 22 Where R ¹ Is n-butyl and R ² Is 2-Methylpropyl

By following the procedures described in

Example 19A and substituting MeS (NCbz) ₂ - (CH ₂ -DL-Leu-CH ₂ ) -

OTBS with n-BuS (NCbz) ₂ -(CH ₂ -LL-Leu-CH ₂ ) -OTBS, 287 mg (65% yield) of n-BuS (NCbz) ₂ - (CH ₂ -DL-Leu-CH ₂ ) -OH was obtained as a gum. Characteristic analytical data are as follows: ² H NMR (300 MHz, CDC1 ₃ ) δ 7.27-7.40 (m, 10H) , 5.09 (s, 4H) , 3.82-3.94 (m, 3H) , 3.78 (dd, J=4, 11 Hz, IH) , 3.57 (dd, J=4, 14 Hz, IH) , 3.42 (dd, J=5, 11 Hz, IH) , 2.22 (m, IH) , 1.52-1.79 (m, 4H) , 1.20-1.43 (m, 3H) , 0.91 (t, J=8 Hz, 3H) , 0.85 (d, J=7 Hz, 3H) , 0.84 (d, J=7 Hz, 3H) .

19C. Formula 19 Where R ¹ Is n-butyl and R ² Is 4-Methoxyphenylmethyl

By following the procedures described in Example 19A and substituting MeS (NCbz) ₂ - (CH ₂ -DL-Leu-CH ₂ ) - OTBS with n-BuS (NCbz) ₂ - (CH ₂ - L-TyrOCH ₃ -CH ₂ ) -OTBS, 400 mg (71% yield) of n-BuS (NCbz) ₂ - (CH ₂ -DL-TyrOMe-CH ₂ ) -OH as a gum was obtained. Characteristic analytical data are as follows: ^α H NMR (300 MHz, CDC1 ₃ ) δ 7.27-7.40 (m, 10H) , 7.03 (d, J=9 Hz, 2H) , 6.82 (d, J=9 Hz, 2H) , 5.10 (m, 4H) , 3.99 (dd, J=8, 14 Hz, IH) , 3.78 (s, 3H) , 3.68-3.74 (m, 2H) , 3.40-3.58 (m, 3H) , 2.72 (dd, J=7, 14 Hz, IH) , 2.62 (dd, J=6, 14 Hz, IH) , 2.33 (br m, IH) , 1.78 (br m, IH) , 1.55 (br m, IH) , 1.26 (br m, 2H) , 0.82 (t, J=7 Hz, 3H) .

19C. Formula 22 Varying R ¹ , R ² and R ³

By following the procedures described in Example 19A and substituting MeS (NCbz) ₂ - (CH ₂ - L-Leu-CH ₂ ) - OTBS with other compounds of Formula 21 (where R ¹ , R ² and R ³ are as indicated in the table below) there are obtained the corresponding substituted compounds of Formula 22.

Formula 22

DL

R ¹ R ² Name ethyl H EtS (NCbz) ₂ -(CH ₂ - L- Gly-CH ₂ ) -OH benzyl methyl BnS (NCbz) ₂ - (CH ₂ -DL- Ala-CH ₂ ) -OH phenethyl 2-propyl PhetS (NCbz) ₂ -(CH ₂ -DL- Val-CH ₂ ) -OH blocked 2-butyl (4-hydroxyphenethy1) - 4-hydroxy- S(NCbz) ₂ -(CH ₂ -.DL-Ile- phenethyl CH ₂ ) -OH blocked 2- (4- benzyl 2 (4-imidazolyl) ethyl- imidazolyl) - S(NCbz) ₂ -(CH ₂ -DL-Phe- ethyl CH ₂ )-OH blocked blocked 4- (3-carboxypropyl) - 3-carboxy¬ hydroxypheny1- S (NCbz) ₂ - (CH ₂ -DL-Tyr- propyl methyl CH ₂ )-OH ethyl 4-methoxy¬ EtS (NCbz) ₂ -(CH ₂ - L- phenylmethyl TyrOCH ₃ -CH ₂ ) -OH benzyl blocked 3- BnS (NCbz) ₂ - { CE ₂ -DL- indolylmethyl Trp-CH ₂ )-OH phenethyl phenylethyl PhetS (NCbz) ₂ - (CH ₂ -DL- Phet-CH ₂ ) -OH blocked blocked 4- (4-hydroxyphenethy1) -

4-hydroxy- aminobuty! S (NCbz) ₂ - (CH ₂ -DL-Lys- phenethyl CH ₂ ) -OH blocked 2- (4- blocked 3- 2- (4-imidazolyl) - imidazolyl) - guanylpropyl ethylS (NCbz) ₂ - (CH ₂ -DL- ethyl Arg-CH ₂ ) -OH blocked blocked 4- 3-carboxypropyl- 3-carboxy¬ imidazoylmethyl S(NCbz) ₂ -(CH ₂ -DL-His- propyl CH ₂ )-OH ethyl H EtS (NCbz) ₂ -(CH ₂ -DL- Gly-CH ₂ )-OH

Formula 22

DL

R ¹ R ² Name benzyl methyl BnS (NCbz) ₂ -(CH ₂ - L- Ala-CH ₂ ) -OH phenethyl 2-propyl PhetS (NCbz) ₂ - (CH ₂ -DL- Val-CH ₂ ) -OH blocked 2-butyl 4-hydroxyphenethy1- 4-hydroxy- S(NCbz) ₂ -(CH ₂ -DL-Ile- phenethyl CH ₂ ) -OH blocked 2- (4- benzyl 2- (4-imidazolyl) - imidazolyl) - ethylS (NCbz) ₂ -(CH ₂ - L- ethyl Phe-CH ₂ ) -OH blocked blocked 4- 3-carboxypropyl- 3-carboxy¬ hydroxypheny1- S(NCbz) ₂ -(CH ₂ -DL-Tyr- propyl methyl CH ₂ ) -OH ethyl blocked 3- EtS (NCbz) ₂ - (CH,--D - indolylmethyl Trp-CH ₂ )-OH benzyl phenylethyl BnS (NCbz) ₂ -(CH ₂ -DL- Phet-CH ₂ ) -OH phenethyl blocked 4- PhetS (NCbz) ₂ - (CH ₂ - L- aminobutyl Lys-CH ₂ ) -OH blocked blocked 3- (4-hydroxyphenethy1) - 4-hydroxy¬ guanylpropyl S(NCbz) ₂ -(CH ₂ -DL-Arg- phenethyl CH ₂ )-OH blocked 2- (4- blocked 4- 2- (4-imidazolyl) - imidazolyl) - imidazoylmethy1 ethylS (NCbz) ₂ - (CH ₂ - L- ethyl His-CH ₂ ) -OH blocked H 3-carboxypropy1- 3-carboxy¬ S (NCbz) ₂ - (CH ₂ -DL-Gly- propyl CH ₂ )-OH ethyl methyl EtS(NCbz) ₂ -(CH ₂ -DL- Ala-CH ₂ ) -OH benzyl 2-propyl BnS(NCbz) ₂ - (CH ₂ -DL- Val-CH ₂ )-OH

Formula 22

DL

R ¹ R ² Name phenethyl 2-butyl PhetS (NCbz) ₂ -(CH ₂ -DL- Ile-CH ₂ ) -OH blocked benzyl 4-hydroxyphenethy1- 4-hydroxy- S(NCbz) ₂ -(CH ₂ -DL-Phe- phenethyl CH ₂ ) -OH blocked 2- (4- blocked 4- 2- (4-imidazolyl) - imidazolyl) - hydroxypheny1- ethylS (NCbz) ₂ - (CH ₂ -DL- ethyl methyl Tyr-CH ₂ ) -OH blocked blocked 3- 3-carboxy- 3-carboxy¬ indolylmethyl propylS(NCbz) ₂ -(CH ₂ - propyl I?L-Trp-CH ₂ )-OH ethyl phenylethyl EtS (NCbz) ₂ - (CH ₂ - L- Phet-CH ₂ )-OH benzyl blocked 4- BnS (NCbz) ₂ - (CH ₂ -DL- aminobutyl Lys-CH ₂ )-OH phenethyl blocked 3- PhetS (NCbz) ₂ -(CH ₂ -.DL- guanylpropyl Arg-CH ₂ )-OH blocked blocked 4- 4-hydroxyphen- 4-hydroxy- imidazoylmethyl ethylS (NCbz) ₂ - (CH ₂ -DL- phenethyl His-CH ₂ )-OH blocked 2- (4- H 2- (4-imidazolyl) - imidazolyl) - ethylS (NCbz) ₂ -{CH ₂ --D - ethyl Gly-CH ₂ )-OH blocked methyl 3-carboxy-

3-car )xy- propylS(NCbz) ₂ -(CH ₂ - prc /l DL-Ala-CH ₂ ) -OH ethyl 2-propyl EtS(NCbz) ₂ -(CH ₂ -DL- Val-CH ₂ )-OH benzyl 2-butyl BnS(NCbz) ₂ -(CH ₂ - L- Ile-CH ₂ )-OH phenethyl benzyl PhetS (NCbz) ₂ -(CH ₂ -DL- Phe-CH ₂ )-OH

Formula 22

DL

R ¹ R ² Name blocked blocked 4- 4-hydroxyphen- 4-hydroxy¬ hydroxypheny1- ethylS(NCbz) ₂ -(CH,- L- phenethyl methyl Tyr-CH ₂ ) -OH ^" blocked 2- (4- blocked 3- 2- (4-imidazolyl) - imidazolyl) - indolylmethyl ethylS (NCbz) ₂ - (CH ₂ --DL- ethyl Trp-CH ₂ ) -OH blocked phenylethyl 3-carboxy- 3-carboxy¬ proρylS(NCbz) ₂ - (CH ₂ - propyl DL-Phet-CH ₂ ) -OH

EXAMPLE 20

PREPARATION OF MeS(NCbz) ₂ -(CH ₂ --DIι-Leu) -OH

20A. Formula 23 Where R ¹ Is Methyl and R ² Is 2-Methylpropyl

To a solution of 390 mg of MeS(NCbz) ₂ - (CH ₂ -DL-

Leu-CH ₂ )-OH in 5 mL of acetone was added 0.5 mL of Jones reagent dropwise at 0 ^C C, and stirring was continued for 3 h. The reaction mixture was poured into water and was extracted with CH ₂ C1 ₂ . The organic layer was dried over

Na ₂ S0 ₄ and evaporated under vacuum, and the residue was purified by flash chromatography to give 290 mg (72% yield) of MeS(NCbz) ₂ -(CH ₂ - L-Leu) -OH as a viscous oil: ^α H

NMR (300 MHz, CDC1 ₃ ) δ 7.24-7.40 ( , 10H) , 5.04-5.15 (m,

4H) , 4.09 (dd, J=9, 14 Hz, IH) , 3.88 (d, J=14 Hz, IH) ,

3.42 (s, 3H) , 3.05-3.16 (m, IH) , 1.54-1.68 ( , 2H) , 1.32-

1.45 ( , IH) , 0.88 (d, J=6 Hz, 3H) , 0.86 (d, J=6 Hz, 3H) .

2OB. Formula 23 Where R ¹ Is n-Butyl and R ² Is 2-Methylpropyl

By following the procedures described in

Example 20A and substituting MeS(NCbz) ₂ - (CH ₂ - L-Leu-CH ₂ ) - OH with n-BuS(NCbz) ₂ -(CH ₂ -DL-TyrOMe-CH ₂ ) -OH, 185 mg (72% yield) of n-BuS(NCbz) ₂ - (CH ₂ -DL-Leu) -OH was obtained as a viscous oil. Characteristic analytical data are as follows: ^α H NMR (300 MHz, CDC1 ₃ ) δ 7.27-7.40 (m, 10H) ,

5.12 (d, J=12 Hz, IH) , 5.10 (s, 2H) , 5.06 (d, J=12 Hz, IH) , 4.1 (m, IH) , 3.8 (m, 2H) , 3.6 (m, IH) , 3.16 (m, IH) ,

1.55-1.76 (m, 4H) , 1.37 (m, 3H) , 0.88 (t, J=7 Hz, 3H) ,

0.87 (d, J=6 Hz, 3H) , 0.85 (d, J=6 Hz, 3H) .

20C. Formula 23 Where R ¹ Is n-Butyl and R ² Is 4-Methoxyphenylmethyl

By following the procedures described in

Example 20A and substituting MeS(NCbz) ₂ - (CH ₂ --DL-Leu-CH ₂ )-

OH with n-BuS(NCbz) ₂ -(CH ₂ -DL-TyrOMe-CH ₂ )-OH, 238 mg (58% yield) of n-BuS(NCbz) ₂ -(CH ₂ -DL-TyrOMe) -OH was obtained as a viscous oil. Characteristic analytical data are as follows: ^l E NMR (300 MHz, CDC1 ₃ ) δ 7.24-7.39 (m, 10H) ,

7.02 (d, J=9 Hz, 2H) , 6.78 (d, J=9 Hz, 2H) , 5.03 (m, 4H) ,

4.20 (m, IH) , 3.72 (s, 3H) , 3.54 (m, 3H) , 3.22 (m, IH) ,

3.05 (m, IH) , 2.75 (m, IH) , 1.5 (m, IH) , 1.21 (m, 3H) , 0.75 (t, J=7 Hz, 3H) .

20C. Formula 23 Varying R ¹ , R ² and R ³

By following the procedures described in Example 20A and substituting MeS(NCbz) ₂ - (CH ₂ -DL-Leu-CH ₂ ) - OH with other compounds of Formula 22 (where R ¹ , R ² and R ³ are as indicated in the table below) there are obtained the corresponding substituted compounds of Formula 23.

Formula 23

R ¹ R ² Name ethyl H EtS (NCbz) ₂ -(CH ₂ -DL- Gly) -OH benzyl methyl BnS (NCbz) ₂ - (CH ₂ -DL- Ala)-OH phenethyl 2-propyl PhetS (NCbz) ₂ -(CH ₂ -DL- Val)-OH blocked 2-butyl ( -hydroxyphenethy1) - 4-hydroxy- S(NCbz) ₂ -(CH ₂ -Iλ -Ile) - phenethyl OH blocked 2- (4- benzyl 2 (4-imidazolyl) ethyl- imidazolyl) - S(NCbz) ₂ -(CH ₂ -.DL-Phe) - ethyl OH blocked blocked 4- (3-carboxypropyl)S- 3-carboxy¬ hydroxypheny1- (NCbz) ₂ -(CH ₂ -.DL-Tyr) - propyl methyl OH ethyl 4-methoxy¬ EtS (NCbz) ₂ -(CH ₂ -DL- phenylmethyl TyrOCH ₃ ) -OH benzyl blocked 3- BnS (NCbz) ₂ -(CH ₂ -DL- indolylmethyl Trp) -OH phenethyl phenylethyl PhetS (NCbz) ₂ -(CH ₂ -DL- Phet) -OH blocked blocked 4- (4-hydroxyphenethy1) - 4-hydroxy- a inobutyl S(NCbz) ₂ - (CH ₂ -DL-Lys)- phenethyl OH blocked 2- (4- blocked 3- 2-(4- imidazolyl) - guanylpropyl imidazolyl)ethyl- ethyl S(NCbz) ₂ -(CH ₂ -IλL-Arg) -

OH blocked blocked 4- 3-carboxypropyl- 3-carboxy¬ imidazoylmethyl S(NCbz) ₂ -(CH ₂ -DL-His)- propyl OH

Formula 23

R ¹ R ² Name ethyl H EtS (NCbz) ₂ -(CH ₂ -DL- Gly) -OH benzyl methyl BnS (NCbz) ₂ - (CH ₂ --D - Ala)-OH phenethyl 2-propyl PhetS (NCbz) ₂ - (CH ₂ -DL- Val) -OH blocked 2-butyl 4-hydroxyphenethy1- 4-hydroxy- S (NCbz) ₂ - (CH ₂ --D -Ile) - phenethyl OH blocked 2- (4- benzyl 2- (4-imidazolyl) - imidazolyl) - ethylS (NCbz) ₂ - (CH ₂ -nL- ethyl Phe) -OH blocked blocked 4- 3-carboxypropyl- 3-carboxy¬ hydroxypheny1- S(NCbz) ₂ -(CH ₂ -DL-Tyr) - propyl methyl OH ethyl blocked 3- EtS (NCbz) ₂ -(CH ₂ -DL- indolylmethyl Trp) -OH benzyl phenylethyl BnS (NCbz) ₂ -(CH ₂ -DL- Phet) -OH phenethyl blocked 4- PhetS (NCbz) ₂ -(CH ₂ -DL- aminobutyl Lys) -OH blocked blocked 3- (4-hydroxyphenethy1) - 4-hydroxy- guanylpropyl S (NCbz) ₂ - (CH ₂ -L>L-Arg) - phenethyl OH blocked 2- (4- blocked 4- 2- (4-imidazolyl) - imidazolyl) - imidazoylmethyl ethylS (NCbz) ₂ -(CH ₂ -DL- ethyl His)-OH blocked H 3-carboxypropyl- 3-carboxy¬ S(NCbz) ₂ -(CH ₂ -DL-Gly) - propyl OH

Formula 23

R ¹ R ² Name ethyl methyl EtS (NCbz) ₂ -(CH ₂ -DL- Ala) -OH benzyl 2-propyl BnS (NCbz) ₂ -(CH ₂ -DL- Val) -OH phenethyl 2-butyl PhetS (NCbz) ₂ -(CH ₂ -DL- Ile) -OH blocked benzyl 4-hydroxyphenethy1- 4-hydroxy- S (NCbz) ₂ - (CH ₂ - L-Phe) - phenethyl OH blocked 2- (4- blocked 4- 2- (4-imidazolyl) - imidazolyl) - hydroxypheny1- ethylS (NCbz) ₂ -(CH ₂ - L- ethyl methyl Tyr) -OH blocked blocked 3- 3-carboxypropyl- 3-carboxy¬ indolylmethyl S(NCbz) ₂ -(CH ₂ -DL-Trp) - propyl OH ethyl phenylethyl EtS (NCbz) ₂ -(CH ₂ -DL- Phet) -OH benzyl blocked 4- BnS (NCbz) ₂ -(CH ₂ -DL- aminobutyl Lys) -OH phenethyl blocked 3- PhetS (NCbz) ₂ - (CH ₂ -Z?L- guanylpropyl Arg)-OH blocked blocked 4- 4-hydroxyphenethy1- 4-hydroxy- imidazoylmethyl S(NCbz) ₂ -(CH ₂ -DL-His) - phenethyl OH blocked 2- (4- H 2- (4-imidazolyl) - imidazolyl) - ethylS (NCbz) ₂ -(CH ₂ -DL- ethyl Gly)-OH blocked methyl 3-carboxypropyl- 3-carboxy¬ S (NCbz) ₂ - (CH ₂ -DL-Ala) - propyl OH

Formula 23

R ¹ R ² Name ethyl 2-propyl EtS (NCbz) ₂ - (CH ₂ -DL- Val) -OH benzyl 2-butyl BnS (NCbz) ₂ -(CH ₂ -I5L- Ile) -OH phenethyl benzyl PhetS (NCbz) ₂ -(CH ₂ -DL- Phe)-OH blocked blocked 4- 4-hydroxyphenethy1-

4-hydroxy- hydroxypheny1- S (NCbz) ₂ - (CH ₂ - L-Tyr) - phenethyl methyl OH blocked 2- (4- blocked 3- 2- (4-imidazolyl) - imidazolyl) - indolylmethyl ethylS (NCbz) ₂ - (CH ₂ -DL- ethyl Trp)-OH blocked phenylethyl 3-carboxypropyl- 3-carboxy¬ S(NCbz) ₂ -(CH ₂ - L- propyl Phet) -OH

EXAMPLE 21 PREPARATION OF MeS(NCbz) ₂ -(CH ₂ --DIι-Leu)-Trp-NHBn

21A. Formula 24 Where R ¹ Is Methyl, R ² Is 2-Methylpropyl, R ³ s 3-Indolylmethyl and R ⁴ Is Benzyl

MeS(NCbz) ₂ -(CH ₂ -DL-Leu)-OH and Trp-NHBn were coupled and the resulting mixture of two diastereomers was separated by flash chromatography on silica gel using 40% EtOAc in toluene. The faster-eluting diastereomer of MeS(NCbz) ₂ -(CH ₂ -L>L-Leu) -Trp-NHBn was isolated in 39% yield. Characteristic analytical data are as follow: mp 87-97°C (lyophilized powder); *H NMR (300 MHz, CDC1 ₃ ) δ 7.74 (br s, IH) , 7.63 (d, J=7 Hz, IH) , 7.01-7.41 (m,

18H) , 6.86 (d, J=3 Hz, IH) , 6.49 (d, J=8 Hz, IH) , 5.98

(br t, J=6 Hz, IH) , 5.10 (s, 2H) , 5.08 (s, 2H) , 4.67 (q, J=8 Hz, IH) , 4.29 (m, 2H) , 3.81 (m, 2H) , 3.24 (dd, J=7, 15 Hz, IH), 3.15 (dd, J=8, 15 Hz, IH) , 2.98 (s, 3H) , 2.82-2.91 (m, IH) , 1.38-1.50 (m, 2H) , 1.21-1.28 (m, IH) , 0.78 (d, J=6 Hz, 3H) , 0.77 (d, J=6 Hz, 3H) ; mass spectrum (PCI), m/e 404 (MH ⁺ - MeS(NCbz) ₂ H, 100); [α] ²⁵ _D -75° (c = 0.12, MeOH). The slower-eluting diastereomer of MeS(NCbz) ₂ - (CH ₂ - L-Leu) -Trp-NHBn was obtained in 23% yield: mp 68-78°C (lyophilized powder); ^α H NMR (300 MHz, CDC1 ₃ ) δ 7.92 (br s, IH) , 7.62 (d, J=8 Hz, IH) , 7.10-7.34 (m, 18H) , 6.96 (t, J=7 Hz, IH) , 6.89 (d, J=2 Hz, IH) , 6.02 (d, J=8 Hz, IH) , 5.02 (s, 2H) , 4.95 (s, 2H) , 4.74 (q, J=7 Hz, IH) , 4.47 (dd, J=7, 15 Hz, IH) , 4.12 (dd, J=5, 15 Hz, IH) , 3.93 (dd, J=10, 14 Hz, IH) , 3.64 (d, J=14 Hz, IH) , 3.34 (dd, J=6, 15 Hz, IH) , 3.19 (dd, J=8, 15 Hz, IH) , 3.18 (s, 3H) , 2.65-2.76 (m, IH) , 1.04-1.30 (m, 3H) , 0.63 (d, J=7 Hz, 3H) , 0.58 (d, J=6 Hz, 3H) ; mass spectrum (PCI), m/e 404 (MH ⁺ - MeS(NCbz) ₂ H, 100); [CC] ²⁵ _D - 25° (c = 0.14, MeOH) .

21B. Formula 24 Where R ¹ Is n-Butyl, R ² Is

2-Methylpropyl, R ³ Is Benzyl and R ⁵ Is Methyl

By following the procedures described in

Example 21A and substituting MeS(NCbz) ₂ - (CH ₂ -.DL-Leu) -OH and Trp-NHBn with n-BuS(NCbz) ₂ - (CH ₂ - L-Leu) -OH and Phe-

Ala-NH ₂ , -n-BuS(NCbz) ₂ - (CH ₂ -L>L-Leu)-Phe-Ala-NH ₂ was obtained as a mixture of two diastereomers. Separation into individual diastereomers was achieved using preparative thin-layer chromatography on silica gel (eluting three times with CHCl ₃ :EtOH 30:1). Characteristic analytical data are as follows for the faster-eluting diastereomer: 'H NMR (300 MHz, CDC1 ₃ ) δ 7.53 (d, J=7 Hz, IH, NH) , 7.15- 7.39 ( , 15H) , 6.59 (br s, IH, NH) , 6.26 (d, J=7 Hz, IH, NH) , 5.14 (d, J=12 Hz, IH) , 5.08 (s, 2H) , 5.04 (d, J=12 Hz, IH) , 4.93 (br s, IH, NH) , 4.67 (m, IH) , 4.34 (p, J=7 Hz, IH) , 4.15 (dd, J=ll, 13 Hz, IH) , 3.65-3.88 (m, 2H) ,

3.29-3.38 (m, 2H) , 2.75 (dd, J=10, 14 Hz, IH) , 2.7-2.8 (br m, IH) , 1.54-1.75 (m, 4H) , 1.33 (d, J=7 Hz, 3H) , 1.22-1.44 (m, 3H) , 0.90 (t, J=7 Hz, 3H) , 0.67 (d, J=7 Hz, 3H) , 0.60 (d, J=6 Hz, 3H) ; mass spectrum (PCI), m/e 346 (MH ⁺ -BuS(NCbz) ₂ H, 100) ; [α] ²⁵ ₅₄₆ -107° (c = 0.026, MeOH) . Characteristic analytical data for the slower-eluting diastereomer are as follows: ^* H NMR (300 MHz, CDC1 ₃ ) δ 7.14-7.39 (m, 16H) , 6.41 (d, J=8 Hz, IH, NH) , 6.24 (br s, IH, NH) , 5.30 (br s, IH, NH) , 5.10 (s, 4H) , 4.51 (q, J=7 Hz, IH) , 4.40 (p, J=8 Hz, IH) , 3.91 (dd, J=10, 14 Hz, IH) , 3.65 (m, IH) , 3.55 (dd, J=2, 14 Hz, IH) , 3.29 (m, IH) , 3.05 (m, IH) , 2.98 (d, J=8 Hz, 2H) , 1.57-1.70 (m, 4H) , 1.21-1.56 (m, 3H) , 1.25 (d, J=7 Hz, 3H) , 0.87 (t, J=7 Hz, 3H) , 0.81 (d, J=6 Hz, 3H) , 0.78 (d, J=6 Hz, 3H) ; mass spectrum (PCI), m/e 346 (MH ⁺ -BuS (NCbz) ₂ H, 100) ;

[α] ²⁵ 5 ₅ 4 ₄ 6 ₆ -23° (c = 0.048, MeOH)

21C. Formula 24 Where R ¹ Is n-Butyl, R ² Is 4-Methoxy- phen lmethyl, R ³ Is 3-Indolylmethyl and R ⁴ Is Benzyl By following the procedures described in

Example 21A and substituting MeS (NCbz) ₂ - (CH ₂ -DL-Leu) -OH with n-BuS(NCbz) ₂ -(CH ₂ -DL-TyrOMe)-OH, n-BuS (NCbz) ₂ - (CH ₂ -DL- TyrOMe) -Trp-NHBn was obtained as a mixture of two diastereomers, which were separated by flash chromatography on silica gel (35% ethyl acetate in hexane) . The faster-eluting diastereomer of n-BuS (NCbz) ₂ - (CH ₂ --DL-TyrOMe) -Trp-NHBn was isolated in 43% yield. Characteristic analytical data are as follows: ^X H NMR (300 MHz, CDC1 ₃ ) δ 7.84 (s, IH) , 7.65 (d, J=8 Hz, IH) , 7.04-7.40 (m, 16H) , 6.95 (m, 2H) , 6.90 (d, J=3 Hz, IH) , 6.86 (d, J=9 Hz, 2H) , 6.61 (d, J=9 Hz, 2H) , 6.24 (d, J=8 Hz, IH) , 5.56 (t, J=6 Hz, IH) , 5.10 (m, 4H) , 4.54 (q, J=7 Hz, IH) , 4.17 (dd, J=3, 6 Hz, 2H) , 4.02 (dd, J=10, 14 Hz, IH) , 3.60 (s, 3H) , 3.5-3.64 (m, 2H) , 3.04-3.22 (m, 4H) , 2.77 (dd, J=9, 14 Hz, IH) , 2.66 (dd, J=7, 14 Hz, IH) , 1.53 (m, 2H), 1.11 (m, 2H) , 0.80 (t, J=7 Hz, 3H) . The

slower-eluting diastereomer of n-BuS (NCbz) ₂ - (CH ₂ -DL- TyrOMe) -Trp-NHBn was obtained in 42% yield. Characteristic analytical data are as follows: ^l E NMR (300 MHz, CDC1 ₃ ) δ 7.98 (s, IH) , 7.38 (d, J=8 Hz, IH) , 6.98-7.34 (m, 19H) , 6.91 (d, J=9 Hz, 2H) , 6.78 (d, J=9

Hz, 2H) , 2.60 (d, J=3 Hz, IH) , 6.07 (d, J=8 Hz, IH) , 4.93 (s, 2H) , 4.87 (d, J=13 Hz, IH) , 4.76 (d, J=13 Hz, IH) , 4.63 (q, J=6 Hz, IH) , 4.37 (dd, J=6, 15 Hz, IH) , 3.99- 4.16 (m, 2H) , 3.75 (s, 3H) , 3.41-3.52 ( , 2H) , 3.31 (m, IH) , 3.19 (dd, J=6, 15 Hz, IH) , 2.94 (m, 2H) , 2.72 (dd,

J=6, 13 Hz, IH) , 2.59 (dd, J=7, 13 Hz, IH) , 1.48 (m, 2H) , 1.22 (m, 2H) , 0.80 (t, J=7 Hz, 3H) .

21D. Formula 24 Where R ¹ Is n-Butyl, R ² Is 2-Methyl- propyl, R ³ Is 3-Indolylmethyl and R ⁴ Is Benzyl

By following the procedures described in

Example 21A and substituting MeS (NCbz) ₂ - (CH ₂ -I -Leu) -OH with n-BuS(NCbz) ₂ - (CH ₂ -DL-Leu) -OH, n-BuS (NCbz) ₂ - (CH ₂ -JDL-

Leu) -Trp-NHBn was obtained as a mixture of two diastereomers, which were separated by flash chromatography on silica gel (20% EtOAc in toluene) . The faster-eluting diastereomer of n-BuS (NCbz) ₂ - (CH ₂ -DL-Leu) - Trp-NHBn was isolated in 45% yield as a solid. Characteristic analytical data are as follows: mp 76- 78°C (ether); ^X H NMR (300 MHz, CDC1 ₃ ) δ 7.84 (br s, IH, NH) , 7.65 (d, J=8 Hz, IH) , 7.06-7.40 (m, 16H) , 7.0 (m, 2H) , 6.92 (d, J=2 Hz, IH) , 6.51 (br d, J=7 Hz, IH, NH) , 5.95 (br t, J=5 Hz, IH, NH) , 5.14 (d, J=13 Hz, IH) , 5.09 (s, 2H) , 5.07 (d, J=13 Hz, IH) , 4.59 (q, J=7 Hz, IH) , 4.28 (dd, J=5, 15 Hz, IH) , 4.26 (dd, J=6, 15 Hz, IH) ,

3.90 (dd, J=10, 14 Hz, IH) , 3.51-3.70 (m, 2H) , 3.25 (dd, J=7, 14 Hz, IH) , 3.16 (dd, J=7, 15 Hz, IH) , 3.0 (m, IH) , 2.84 (m, IH) , 1.56 (m, 2H) , 1.43 ( , 2H) , 1.25 (m, IH) , 1.07 (m, 2H) , 0.78 (t, J=7 Hz, 3H) , 0.75 (d, J=6 Hz, 6H) ; mass spectrum (PCI), m/e 404 (MH ⁺ -BuS (NCbz) ₂ H, 44) ; [α] ⁵ _D -56° (c = 0.18, MeOH) . The slower-eluting diastereomer

of n-BuS (NCbz) ₂ - (CH ₂ -DL-Leu) -Trp-NHBn was also isolated in 45% yield as a solid. Characteristic analytical data are as follows: mp 59-61°C (ether) ; ^X H NMR (300 MHz, CDC1 ₃ ) δ 8.01 (br s, IH, NH) , 7.61 (d, J=8 Hz, IH) , 7.08-7.32 (m, 19H) , 6.88 (d, J=2 Hz, IH) , 6.0 (br m, IH, NH) , 5.0 (s, 2H) , 4.95 (d, J=12 Hz, IH) , 4.90 (d, J=12 Hz, IH) , 4.77

(q, J=8 Hz, IH) , 4.46 (dd, J=6, 15 Hz, IH) , 4.14 (dd, J=4, 15 Hz, IH) , 3.97 (dd, J=ll, 14 Hz, IH) , 3.32-3.62

(m, 4H) , 3.17 (dd, J=8, 15 Hz, IH) , 2.70 (m, IH) , 1.52 (m, 2H) , 1.02-1.32 (m, 5H) , 0.82 (t, J=7 Hz, 3H) , 0.61

(d, J=6 Hz, 3H) , 0.55 (d, J=6 Hz, rf) ; mass spectrum

(PCI), m/e 404 (MH ⁺ -BuS (NCbz) ₂ H, 82); [α] ²⁵ ₅₄₆ +2.2° (c = 0.12, MeOH) .

2IE. Formula 24 Varying R ¹ , R ² , R ³ , R ⁴ and R ⁵

By following the procedures described in Examples 21A and substituting MeS (NCbz) ₂ - (CH ₂ -DL-Leu) -OH with other compounds of Formula 23 (e.g., compounds with R ¹ and R ² that are prepared according to Example 21A and exemplified with Example 21D) and Trp-NHBn with other compounds of Formula 6 (e.g., compounds with R ³ , R ⁴ and R ⁵ that are prepared according to Example 5A and exemplified with Example 5B) , for example there are obtained the following correspondingly substituted compounds of Formula 24.

Formula 24

where R ⁴ is -CH ₃ or Bn

R ¹ R ² R ³ R ⁴ ethyl H H -CH ₃ benzyl methyl methyl -Bn phenethyl 2-propyl 2-propyl -CH ₃ blocked 2-butyl 2-butyl -Bn

4-hydroxyphen- ethyl blocked 2- (4- benzyl 2-methyl¬ -CH ₃ imidazolyl)ethyl propyl blocked blocked 4- blocked 4- -Bn 3-carboxypropyl hydroxypheny1- aminobutyl methyl blocked 4-methoxy¬ blocked 3- -CH ₃ 3-carboxypropyl phenylmethyl guanylpropyl blocked 2- (4- blocked 3- blocked 4- -Bn imidazolyl) ethyl indolylmethyl imidazoyl- methyl blocked phenylethyl benzyl -CH ₃

4-hydroxyphen- ethyl phenethyl blocked 4- blocked -Bn aminobutyl 4-hydroxy¬ phenylmethyl benzyl blocked 3- 3-indoyl- -CH ₃ guanylpropyl methyl ethyl blocked 4- 4-methoxy¬ -Bn i idazoyl- phenylmethyl ethyl blocked H phenylethyl -CH ₃ 3-carboxypropyl

Formula 24

where R ⁴ is -CH ₃ or Bn

R ¹ R ² R ³ R ⁴ blocked 2- (4- methyl thiolmethyl -Bn imidazolyl) ethyl blocked 2-propyl methyl- -CH ₃

4-hydroxyphen- thioethyl ethyl phenethyl 2-butyl methyl- -Bn thioethyl benzyl benzyl thiolmethyl -CH ₃ ethyl blocked 4- phenylethyl -Bn hydroxyphenylm ethyl ethyl 4- 4-methoxy¬ -CH ₃ methoxyphenylm phenylmethyl ethyl benzyl blocked 3- 3-indoyl- -Bn indolylmethyl methyl phenethyl phenylethyl blocked -CH ₃ 4-hydroxy¬ phenylmethyl blocked 4- blocked 4- benzyl -Bn hydroxyphenethyl aminobutyl blocked blocked 3- blocked 4- -CH ₃

2 - (4-imidazolyl) guanylpropyl imidazoyl- ethyl methyl blocked blocked 4- blocked 3- -Bn 3-carboxypropyl i idazoyl- guanylpropyl methyl blocked H blocked 4- -CH ₃ 3-carboxypropyl aminobutyl

where R ⁴ is -CH, or Bn

R ¹ R ² R ^J R ⁴ blocked methyl 2 -methyl¬ -Bn

2 - ( 4-imidazolyl ) propyl ethyl blocked 4- 2-propyl 2-butyl -CH, hydroxyphenethyl phenethyl 2-butyl 2-propyl -Bn benzyl benzyl methyl -CH ₃ ethyl blocked 4- H -Bn hydroxypheny1- methyl

Formula 24

where R ⁴ is -CH ( R ⁵ ) -C ( 0 ) NH ₂

R ¹ R^ R ^J R ethyl H H methyl- thioethyl benzyl methyl methyl thiolmethyl phenethyl 2-propyl 2-propyl phenylethyl blocked 2-butyl 2-butyl 4-methoxy¬ -hydroxyphen- phenylmethyl ethyl blocked 2- (4- benzyl 2-methyl¬ 3-indoyl- imidazolyl) - propyl methyl ethyl blocked 3- blocked blocked 4- blocked 4- carboxypropyl 4-hydroxy¬ aminobutyl hydroxy- phenyl- phenylmethyl methyl blocked 3- 4-methoxy¬ blocked 3- benzyl carboxypropyl phenyl- guanyl- methyl propyl blocked 2-(4- blocked blocked 4- blocked 4- imidazolyl) - 3-indolyl- imidazoyl- imidazoyl- ethyl methyl methyl methyl blocked 4- phenylethyl benzyl blocked - hydroxy- 3-guanyl- phenethyl propyl phenethyl blocked blocked blocked

4-amino- 4-hydroxy¬ 4-amino- butyl phenyl- butyl methyl benzyl blocked 3-indoyl- 2-methyl¬

3-guanyl- methyl propyl propyl

Formula 24

where R ⁴ is -CH (R ⁵ ) -C (0)NH ₂

R ¹ R ^J R ethyl blocked 4- -methoxy¬ 2-butyl imidazoyl- phenyl- methyl methyl blocked H phenyl¬ 2-propyl -carboxypropyl ethyl blocked 2- (4- methyl thiol¬ methyl imidazolyl) - methyl ethyl blocked 2-propyl methyl- H -hydroxyphen- thioethyl ethyl phenethyl 2-butyl methyl- H thioethyl benzyl benzyl thiol¬ methyl methyl ethyl blocked phenyl¬ 2-propyl 4-hydroxy¬ ethyl phenyl- methyl ethyl 4-methoxy¬ 4-methoxy¬ 2-butyl phenyl- phenyl- methyl methyl benzyl blocked 3-indoyl- 2-methyl¬

3-indolyl- methyl propyl methyl phenethyl phenylethyl blocked blocked 4-hydroxy¬ 4-aminobutyl phenyl- methyl

Formula 24

where R ⁴ is -CH(R ⁵ ) -C (0)NH ₂

R ¹ R ^J R ^b blocked blocked benzyl blocked -

4-hydroxyphe - 4-amino- 3-guanyl- ethyl butyl propyl blocked 2- (4- blocked blocked 4- blocked imidazolyl) - 3-guanyl- imidazoyl- 4-imidazoyl- ethyl propyl methyl methyl blocked blocked blocked 3- benzyl -carboxypropyl 4-imidazoyl guanyl- methyl propyl blocked H blocked 4- blocked 3-carboxypropyl aminobutyl 4-hydroxy¬ phenylmethyl blocked 2-(4- methyl 2-methyl¬ 3-indoyl- imidazolyl) - propyl methyl ethyl blocked 2-propyl 2-butyl 4-ethoxy-

4-hydroxyphen- phenylmethyl ethyl phenethyl 2-butyl 2-propyl phenylethyl benzyl benzyl methyl thiolmethyl ethyl blocked H methyl- 4-hydroxy¬ thioethyl phenyl- methyl

EXAMPLE 22

PREPARATION OF MeS(NH) ₂ -(CH ₂ --DIr-Leu)-Trp-NHBn

22A. Formula I Where X Is NH, R ¹ Is Methyl, R ² Is 2-Methylpropyl, R ³ Is 3-Indolylmethyl and R ⁴ Is Benzyl

To 21 mg (0.207 mmol) of MeS (NCbz) ₂ - (CH ₂ -DL-

Leu) -Trp-NHBn (faster-eluting diastereomer) in 5 mL of

EtOH was added 9 mg of 10% Pd/C and 2 drops of cyclohexylamme, and hydrogen gas was bubbled through the mixture for 3 h. The reaction mixture was filtered and the solid was washed with EtOH. The combined filtrates were evaporated under vacuum and the residue was purified by flash chromatography (10% MeOH in CH ₂ C1 ₂ ) to give 11 mg (86% yield) of MeS (NH) ₂ - (CH ₂ -D -Leu) -Trp-NHBn (faster- eluting diastereomer) as a solid: mp 94-96°C; ^: H NMR (300 MHz, CDC1 ₃ ) δ 8.36 (s, IH) , 7.68 (d, J=8 Hz, IH) , 7.36 (d, J=8 Hz, IH) , 7.02-7.30 (m, 9H) , 6.68 (t, J=6 Hz, IH) ,

4.77 (q, J=7 Hz, IH) , 4.35 (dd, J=6, 15 Hz, IH) , 4.30 (dd, J=6, 15 Hz, IH) , 3.22-3.42 (m, 3H) , 2.88 (m, 2H) , 2.63 (s, 3H) , 1.4-1.6 (m, 2H) , 1.2 (m, IH) , 0.85 (d, J=7 Hz, 3H) , 0.82 (d, J=7 Hz, 3H) ; mass spectrum (PCI) , m/e 404 (MH ⁺ - MeS(NH) ₂ H, 100) .

The same procedure applied to the slower- eluting diastereomer of MeS (NCbz) ₂ - (CH ₂ -DI--Leu) -Trp-NHBn afforded an 81% yield of MeS (NH) ₂ - (CH ₂ -D -Leu) -Trp-NHBn (slower-eluting diastereomer) : mp 92-94°C; ^* H NMR (300 MHz, CDC1 ₃ ) δ 8.12 (s, IH) , 7.73 (t, J=5 Hz, IH) , 7.65 (d, J=7 Hz, IH) , 7.39 (d, J=5 Hz, IH) , 7.1-7.3 (m, 7H) , 7.02 (d, J=3 Hz, IH) , 6.48 (d, J=8 Hz, IH) , 4.87 (q, J=6 Hz, IH) , 4.35 (dd, J=6, 15 Hz, IH) , 4.30 (dd, J=6, 15 Hz, IH) , 3.67 (dd, J=ll, 14 Hz, IH) , 3.46 (dd, J=6, 15 Hz, IH) , 3.31 (dd, J=6, 15 Hz, IH) , 2.86 (d, J=12 Hz, IH) ,

2.78 (s, 3H) , 2.70 (m, IH) , 1.5 (m, IH) , 1.26 (ml), 1.14 (m, IH) , 0.76 (d, J=7 Hz, 3H) , 0.74 (d, J=7 Hz, 3H) ; mass spectrum (PCI), m/e 404 (MH ⁺ -MeS(NH) ₂ H, 100) .

22B. Formula I Where X Is NH, R ¹ Is n-Butyl, R ² Is 2-Methylpropyl, R ³ Is Benzyl and R ⁵ Is Methyl

By following the procedures described in

Example 22A and substituting MeS (NCbz) ₂ - (CH ₂ -D -Leu) -Trp- NHBn with n-BuS (NCbz) ₂ - (CH ₂ -D -Leu) -Phe-Ala-NH ₂ , 4.5 mg

(95% yield) of the faster-eluting diastereomer of n-BuS (NH) ₂ - (CH ₂ --D -Leu) -Phe-Ala-NH ₂ was obtained.

Characteristic analytical data are as follows: ^X H NMR

(300 MHz, CDC1 ₃ ) δ 8.23 (br d, J=8 Hz, IH, NH) , 7.19-7.38 (m) , 6.82 (br s, IH, NH) , 6.34 (br d, J=7 Hz, IH, NH) , 5.32 (br s, IH, NH) , 4.52-4.63 (m, 2H) , 3.61 (dd, J=ll, 14 Hz, IH) , 3.38 (dd, J=5, 14 Hz, IH) , 2.91-3.03 (m, 3H) , 2.66-2.80 (m, 2H) , 1.36-1.58 (m, 3H) , 1.32 (d, J=7 Hz, 3H) , 1.22-1.30 ( , IH) , 0.94 (t, J=8 Hz, 3H) , 0.90-1.22 (m, 3H) , 0.75 (d, J=7 Hz, 3H) , 0.66 (d, J=6 Hz, 3H) ; mass spectrum (PCI), m/e 466 (MH ⁺ , 17), 346 (MH ⁺ -BuS (NH) ₂ H, 100) .

The same procedure applied to the slower- eluting diastereomer of n-BuS (NCbz) ₂ - (CH ₂ -D -Leu) -Phe-Ala- NH ₂ afforded a 66% yield of n-BuS (NH) ₂ - (CE ₂ -DL-heu) -Phe- Ala-NH ₂ (slower-eluting diastereomer) : ^* H NMR (300 MHz, CDC1 ₃ ) δ 7.20-7.40 (m, 6H) , 6.96 (br m, IH, NH) , 6.42 (br s, IH, NH) , 5.37 (br s, IH, NH) , 4.44-4.54 (m, 2H) , 3.22- 3.36 (m, 2H) , 3.05 (dd, J=9, 15 Hz, IH) , 2.79-2.97 (m, 3H) , 1.1-1.8 ( , 7H) , 1.35 (d, J=7 Hz, 3H) , 0.97 (t, J=7 Hz, 3H) , 0.88 (d, J=7 Hz, 3H) , 0.81 (d, J=6 Hz, 3H) ; mass spectrum (PCI), m/e 466 (MH ⁺ , 21), 346 (MH ⁺ -BuS (NH) ₂ H, 100) .

22C. Formula I Where X Is NH, R ¹ Is n-Butyl, R ² Is

4-Methoxyphen lmeth l, R ³ Is 3-Indol lmethyl and R ⁴ Is Benzyl

By following the procedures described in

Example 22A and substituting MeS (NCbz) ₂ - (CH ₂ -D -Leu) -Trp- NHBn with n-BuS (NCbz) ₂ - (CH ₂ -D -TyrOMe) -Trp-NHBn, 12 mg

(69% yield) of n-BuS (NH) ₂ - (CH ₂ -C -TyrOMe) -Trp-NHBn

(faster-eluting diastereomer) was obtained as a solid.

Characteristic analytical data are as follows: mp 85- 87°C; ^: H NMR (300 MHz, CD ₃ OD) δ 7.58 (d, J=8 Hz, IH) , 7.30 (d, J=8 Hz, IH) , 7.19-6.93 (m, 10H) , 6.69 (d, J=8 Hz, 2H), 4.56 (t, J=7 Hz, IH) , 4.22 (d, J=15 Hz, IH) , 4.13 (d, J=15 Hz, IH) , 3.65 (s, 3H) , 3.46 (dd, J=10, 14 Hz, IH) , 3.25 (dd, J=7, 15 Hz, IH) , 3.07 (dd, J=8, 15 Hz, 2H) , 2.91-2.76 (m, 2H) , 2.69-2.57 (m, 3H) , 1.57-1.30 (m, 2H) , 1.16 (m, 2H) , 0.79 (t, J=7 Hz, 3H) ; mass spectrum (PCI), m/e 468 (MH ⁺ -BuS (NH) ₂ H, 100) . The same procedure applied to the slower- eluting diastereomer of n-BuS (NCbz) ₂ - (CH ₂ - -TyrOMe) -Trp- NHBn afforded a 75% yield of n-BuS (NH) ₂ - (CH ₂ -D -TyrOMe) - Trp-NHBn (slower-eluting diastereomer) as a solid. Characteristic analytical data are as follows: mp 130- 132°C; -H NMR (300 MHz, CDC1 ₃ ) δ 8.18 (br s, IH, NH) , 7.85 (br t, J=6 Hz, IH, NH) , 7.36 (d, J=9 Hz, 2H) , 6.98-7.24 (m, 10H) , 6.87 (d, J=9 Hz, 2H) , 6.67 (m, IH, NH) , 6.22 (br d, J=9 Hz, IH, NH) , 4.78 (m, IH) , 4.32 (dd, J=6, 15 Hz, IH) , 4.25 (dd, J=5, 15 Hz, IH) , 3.78 (s, 3H) , 3.59 (dd, J=10, 14 Hz, IH) , 3.47 (dd, J=6, 15 Hz, IH) , 2.55- 2.99 (m, 7H) , 1.18-1.52 (m, 4H) , 0.85 (t, J=7 Hz, 3H) ; mass spectrum (PCI), m/e 468 (MH ⁺ -BuS (NH) ₂ H, 100) .

22D. Formula I Where X Is NH, R ¹ Is n-Butyl, R ² Is Isobutyl, R ³ Is 3-Indol lmethyl and R ⁴ Is Benzyl

By following the procedures described in Example 22A and substituting MeS (NCbz) ₂ - (CH ₂ -D -Leu) -Trp- NHBn with n-BuS (NCbz) ₂ - (CH ₂ -D -Leu) -Trp-NHBn, 23 mg (83% yield) of n-BuS (NH) ₂ - (CH ₂ -D -Leu) -Trp-NHBn (faster-eluting diastereomer) was obtained as a solid. Characteristic analytical data are as follows: mp 80-81°C; ^: H NMR (300 MHz, CDC1 ₃ ) δ 8.41 (br s, IH, NH) , 7.68 (d, J=8 Hz, IH) , 7.36 (d, J=8 Hz, IH) , 7.00-7.25 (m, 9H) , 6.80 (br t, J=5 Hz, IH, NH) , 4.76 (q, J=7 Hz, IH) , 4.31 (d, J=6 Hz, 2H) , 3.19-3.41 (m, 3H) , 2.62-2.93 (m, 4H) , 1.40-1.68 (m, 4H) , 1.16-1.39 (m, 3H) , 0.89 (t, J=7 Hz, 3H) , 0.85 (d, J=6 Hz,

3H) , 0.81 (d, J=6 Hz, 3H) ; mass spectrum (PCI), m/e 404 (MH ⁺ -BuS(NH) ₂ H, 100) .

By the same procedure 28 mg of n-BuS (NCbz) ₂ - (CH ₂ -D -Leu) -Trp-NHBn (slower-eluting diastereomer) afforded 18 mg (97% yield) of n-BuS (NH) ₂ - (CH ₂ -D -Leu) -Trp- NHBn (slower-eluting diastereomer) as a solid: mp 143- 145°C; -H NMR (300 MHz, CDC1 ₃ ) δ 8.27 (br s, IH, NH) , 8.03 (br t, J=5 Hz, IH, NH) , 7.65 (d, J=8 Hz, IH) , 7.39 (d, J=8 Hz, IH) , 7.29-7.10 (m, 7H) , 7.0 (d, J=2 Hz, IH) , 6.24 (br d, J=9 Hz, IH, NH) , 4.90 (m, IH) , 4.38 (dd, J=6, 15 Hz, IH) , 4.30 (dd, J=5, 15 Hz, IH) , 3.54 (dd, J=ll, 14 Hz, IH) , 3.47 (dd, J=6, 15 Hz, IH) , 3.30 (dd, J=5, 15 Hz, IH) , 2.82-2.54 (m, 4H) , 1.53 (m, 2H) , 1.30 (m, 4H) , 1.13 (m, IH) , 0.88 (t, J=7 Hz, 3H) , 0.76 (d, J=6 Hz, 3H) , 0.73 (d, J=6 Hz, 3H) ; mass spectrum (PCI), m/e 524 (MH\ 6), 404 (MH ⁺ -BuS(NH) ₂ H, 97) .

22C. Formula I Varying R ¹ , R ² , R ³ , R ⁴ and R ⁵

By following the procedures described in Example 22A and substituting MeS (NCbz) ₂ - (CH ₂ -I -Leu) -Trp- NHBn with other compounds of Formula 24 (where R ¹ , R ² , R ³ , R ⁴ and R ⁵ are as indicated in the table below) , for example there are obtained the corresponding substituted compounds of Formula I.

Formula I

where R ⁴ is -CH ₃ or Bn

R ¹ R ² R ³ R ⁴ ethyl H H -CH ₃ benzyl methyl methyl -Bn phenethyl 2-propyl 2-propyl -CH ₃

4-hydroxyphen- 2-butyl 2-butyl -Bn ethyl - (4-imidazolyl) benzyl 2-methylpropyl -CH ₃ ethyl

3-carboxypropyl 4-hydroxy¬ 4-aminobuty1 -Bn phenylmethyl

3-carboxypropyl 4-methoxy¬ 3-guanylpropyl -CH ₃ phenylmethyl - (4-imidazolyl) 3- 4-imidazoyl- -Bn ethyl indolylmethyl methyl

4-hydroxyphen- phenylethyl benzyl -CH ₃ ethyl phenethyl 4-aminobutyl 4-hydroxy¬ -Bn phenylmethyl benzyl 3-guanylpropyl 3-indoyImethy1 -CH ₃ ethyl 4-imidazoyl- 4-methoxy¬ -Bn methyl phenylmethyl

3-carboxypropyl H phenylethyl -CH ₃ - (4-imidazolyl) methyl thiolmethyl -Bn ethyl

4-hydroxyphen- 2-propyl methyl- -CH ₃ ethyl thioethyl phenethyl 2-butyl methyl- -Bn thioethyl

Formula I

where R ⁴ is -CH ₃ or Bn

R ¹ R ² R ³ R ⁴ benzyl benzyl thiolmethyl -CH ₃ ethyl 4-hydroxy¬ phenylethyl -Bn phenylmethyl ethyl 4-methoxy¬ 4-methoxy¬ -CH ₃ phenylmethyl phenylmethyl benzyl 3-indolyl- 3-indoylmethyl -Bn methyl phenethyl phenylethyl 4-hydroxy¬ -CH ₃ phenylmethyl

4-hydroxyphen- 4-aminobutyl benzyl -Bn ethyl - (4-imidazolyl) 3-guanylpropyl 4-imidazoyl- -CH ₃ ethyl methyl

3-carboxypropyl 4-imidazoyl- 3-guanylpropyl -Bn methyl

3-carboxypropyl H 4-aminobutyl -CH ₃ - (4-imidazolyl) methyl 2-methylpropyl -Bn ethyl

4-hydroxyphen¬ 2-propyl 2-butyl -CH ₃ ethy1 phenethyl 2-butyl 2-propyl -Bn benzyl benzyl methyl -CH ₃ ethyl 4-hydroxy¬ H -Bn phenylmethyl

Formula I

where R ⁴ is -CH(R ⁵ ) -C (0)NH ₂

R ¹ R ² R ³ R ⁵ ethyl H H methyl- thioethyl benzyl methyl methyl thiolmethyl phenethyl 2-propyl 2-propyl phenylethyl -hydroxyphen- 2-butyl 2-butyl 4-methoxy¬ ethyl pheny1- methyl

2-(4- benzyl 2-methyl¬ 3-indoyl- imidazolyl) - propyl methyl ethyl -carboxypropyl 4-hydroxy¬ 4- 4-hydroxy¬ phenyl- aminobutyl phenyl- methyl methyl -carboxypropyl 4-methoxy¬ 3-guanyl¬ benzyl phenyl- propyl methyl

2-(4- 3-indolyl- 4- 4-imidazoyl imidazolyl) - methyl imidazoyl- methyl ethyl methyl -hydroxyphen- phenylethyl benzyl 3-guanylpro ethyl pyl phenethyl 4-amino- 4-hydroxy¬ 4-amino- butyl phenyl- butyl methyl benzyl 3-guanyl¬ 3-indoyl- 2-methyl¬ ropyl methyl propyl ethyl 4- 4-methoxy¬ 2-butyl imidazoyl- phenyl- methyl methyl -carboxypropyl ^H phenylethyl 2-propyl

Formula I

where R ⁴ is -CH(R ⁵ ) -C (0)NH ₂

R ¹ R ² R ³ R ⁵

2-(4- methyl thiolmethyl methyl imidazolyl) - ethyl -hydroxyphen- 2-propyl methyl- H ethyl thioethyl phenethyl 2-butyl methyl- H thioethyl benzyl benzyl thiolmethyl methyl ethyl 4-hydroxy- phenylethyl 2-propyl phenyl¬ methyl ethyl 4-methoxy¬ 4-methoxy¬ 2-butyl phenyl- phenyl- methyl methyl benzyl 3-indolyl- 3-indoyl- 2-methyl¬ methyl methyl propyl phenethyl phenylethyl 4-hydroxy¬ 4-amino- phenyl- butyl methyl -hydroxyphen¬ 4-amino- benzyl 3-guanyl¬ ethy1 butyl propyl

2-(4- 3-guanyl¬ 4- 4- imidazolyl) - propyl imidazoyl- imidazoyl- ethyl methyl methyl -carboxypropyl 4-imidazoyl 3-guanyl¬ benzyl methyl propyl -carboxypropyl H 4- 4-hydroxy¬ aminobutyl phenyl- methyl

Formula I

where R ⁴ is -CH(R ⁵ ) -C (0)NH ₂

R ¹

2-(4- methyl 2-methyl¬ 3-indoyl- imidazolyl) propyl ethyl ethyl

4-hydroxyphen- 2-propyl 2-butyl 4-ethoxy- ethyl phenyl- methyl phenethyl 2-butyl 2-propyl phenylethyl benzyl benzyl methyl thiolmethyl ethyl 4-hydroxy¬ H methyl- phenyl- thioethyl methyl

EXAMPLE 23

PREPARATION OF BnS-(CH ₂ -2-/-Leu-CH ₂ ) -OH 23A. Formula 25 Where R ² Is 2-Methylpropyl

Following the procedure described in Hollady (Hollady, M. W. ; Salituro, F. G.; Rich, D.H., J. Med Chem. 1987, 30, 374-383), to 53 mL of absolute EtOH was added 1.71 g of CaCl ₂ . The mixture was stirred at room temperature under N ₂ until the solid was mostly dissolved. The solution was cooled to 0°C and 1.17 g of NaBH ₄ was added and the mixture was stirred for 0.5 h. To the cold solution was added 6.33 g of (45, 5R) -3- [ ( 2 S) -l-oxo-2-

[ (benzylthio)methyl] -4-methylpentyl] -4-methyl-5-phenyl-2- oxazolidinone in 18 mL of THF dropwise over 15 minutes (an additional 17 mL of THF was used to rinse residual starting material into the reaction flask) . The reaction

mixture was stirred at 0°C under N ₂ for 4 h. The reaction was quenched by addition of 50 mL of EtOAc, 10 mL of H ₂ 0 and 20% HOAc until effervescence ceased. The mixture was acidified to pH 2 with 3 N HCl and the aqueous layer was extracted with EtOAc. The organic extract was washed with saturated NaHC0 ₃ and saturated NaCl, dried over Na ₂ S0 ₄ a:..-! evaporated to give a yellow oil. The crude product was purified by flash chromatography on silica gel (hexane-20% EtOAc) to give 3.19 g (87% yield) of BnS- (CH ₂ - -Leu-CH ₂ ) -OH as a clear oil: R _F 0.3 (hexane-20%

EtOAc) ; ^X H NMR (300 MHz, CDC1 ₃ ) δ 7.33-7.23 (m, 5H) , 3.72 (s, 2H) , 3.69-3.61 (m, IH) 3.58-3.51 (M, IH) , 2.52 (dd, J=6, 13 Hz, IH) 2.47 (dd, J=7, 13 Hz, IH) , 1.82-1.74 (m, IH) , 1.62-1.53 (m, 2H) , 1.15 (dt, J=4, 7 Hz, 2H) , 0.86 (d, J=7 Hz, 3H) 0.85 (d, J=7 Hz, 3H) ; [α] ²⁵ _D -22.6° (c = 3.14, CH ₂ C1 ₂ ) .

23B. Formula 25 Varying R ²

By following the procedures described in Example 23A and substituting (45, 5R) -3- [ (25) -l-oxo-2-

[ (benzylthio)methyl] -4-methylpentyl] -4-methyl-5-phenyl-2- oxazolidinone with other L-configuration compounds of Formula 3 there are obtained the corresponding L- configuration compounds of Formula 25.

EXAMPLE 24 PREPARATION OF BnS-(CH ₂ -L-Leu-CH ₂ ) -OTBS 24A. Formula 26 Where R ² Is 2-Methylprop l

A solution of 2.83 g of BnS- (CH ₂ -L-Leu-CH ₂ ) -OH, 2.16 g of imidazole, and 2.3 g of TBSC1 in 15 mL of dry

DMF was left at room temperature ur-der N ₂ for 7 h. To the mixture was added 200 mL of Et ₂ 0 and 50 mL of H ₂ 0. The organic layer was washed with H ₂ 0, dried over Na ₂ S0 ₄ and evaporated. The resulting yellow oil was purified by flash chromatography on silica gel (hexane-10% EtOAc) to give 3.82 g (91% yield) of BnS- (CH ₂ -L-Leu-CH ₂ ) -OTBS as a

clear oil: R _F 0.8 (hexane-10% EtOAc) ; ^J H NMR (300 MHz, CDC1 ₃ ) δ 7.30-7.21 (m, 5H) , 3.69 (s, 2H) , 3.60 (dd, J=4, 10 Hz, IH) , 3.50 (dd, J=5, 10 Hz, IH) , 2.55 (dd, J=7, 13 Hz, IH) , 2.38 (dd, J=6, 13 Hz, IH) , 1.75-150 ( , 2H) , 1.25-1.10 (m, 2H) , 0.88-0.82 (m, 15H) , 0.02 (s, 6H) ; [α] ²⁵ _D -4.5° (c = 3.57, CH ₂ C1 ₂ ) .

24B. Formula 26 Varying R ²

By following the procedures described in Example 24A and substituting BnS- (CH ₂ -L-Leu-CH ₂ ) -OH with other L-configuration compounds of Formula 25 there are obtained the corresponding L-configuration compounds of FormulaVΩβ.

EXAMPLE 25

PREPARATION OF HS-(CH ₂ -L-Leu-CH ₂ ) -OTBS 25A. Formula 27 Where R ² Is 2-Methylpropyl

Debenzylation of BnS- (CH ₂ -L-Leu-CH ₂ ) -OTBS was carried out using the Na in liquid ammonia procedure of Evans and coworkers (Evans, D.A.; Mathre, D.J.; Scott, .L., J. Org. Chem . 1985, 50, 1830-1835) to give HS-(CH ₂ - L-Leu-CH ₂ ) -OTBS as a clear oil in 88% yield after flash chromatography on silica gel (hexane-10% EtOAc) : R _F 0.3 (hexane-2% EtOAc) ; ^J H NMR (300 MHz, CDC1 ₃ ) δ 3.57 (dd, J=5, 10 Hz, IH) , 3.47 (dd, J=7, 10 Hz, IH) , 2.60 (dd, J=5, 8 Hz, 2H) , 1.78-1.66 (m, IH) , 1.65-1.52 (m, IH) , 1.25-1.10 (m, 3H) , 0.90-0.86 (m, 15H) , 0.04 (s, 6H) ; [α] ²⁵ _D +9.8° (c = 2.7, CH ₂ C1 ₂ ) .

25B. Formula 27 Varying R ²

By following the procedures described in Example 25A and substituting BnS- (CH ₂ -L-Leu-CH ₂ ) -OTBS with other L-configuration compounds of Formula 26 there are obtained the corresponding L-configuration compounds of Formula 27.

EXAMPLE 26 PREPARATION OF MeS-(CH ₂ -Iι-Leu-CH ₂ ) -OTBS 26A. Formula 28 Where R ² Is 2-Methylpropyl

To 25 mL of 0.106 M NaOMe in MeOH was added dropwise a solution of 653 mg of HS- (CH ₂ -L-Leu-CH ₂ ) -OTBS in 9 mL of MeOH. To the solution was added 0.165 L of CH ₃ I and the mixture was allowed to stand at room temperature under N ₂ overnight. The reaction mixture was dilute . with 10 mL of H ₂ 0 and was acidified with 5 mL of 1 N HCl. The MeOH was evaporated under reduced pressure and the aqueous residue was extracted with CH ₂ C1 ₂ . The organic extract was dried over Na ₂ S0 ₄ and evaporated. The resulting yellow oil was purified by flash chromatography on silica gel, eluting first with 100% hexane then with 100% EtOAc. The EtOAc fractions afforded 328 mg of MeS- (CH ₂ -L-Leu-CH ₂ ) -OH. A mixture of 328 mg of MeS-(CH ₂ -L- Leu-CH ₂ )-OH from above, 383 mg of imidazole, and 407 mg of TBSCl in 3 mL of DMF was stirred at room temperature overnight. The reaction mixture was partitioned between 100 mL of Et ₂ 0 and 10 mL of H ₂ 0 and the organic layer was washed with H ₂ 0, dried over Na ₂ S0 ₄ and evaporated. The residue was purified by flash chromatography on silica gel (100% hexane) to give 390 mg (57% yield) of MeS-(CH ₂ - L-Leu-CH ₂ ) -OTBS as a clear oil: R _F 0.15 (hexane) ; ^l E NMR (300 MHz, CDC1 ₃ ) δ 3.63 (dd, J=5, 10 Hz, IH) , 3.53 (dd, J=5, 10 Hz, IH) , 2.60 (dd, J=7, 13 Hz, IH) , 2.44 (dd, J=5, 13 Hz, IH) , 2.08 (s, 3H) , 1.80-1.73 (m, IH) , 1.69- 1.60 (m, IH) , 1.30-1.15 (m, 2H) , 0.90-0.87 (m 15H) , 0.04 (s, 6H) ; [α] ²⁵ _D +2.1° (c = 5.81, CHC1 ₃ ) .

26B. Formula 28 Varying R ²

By following the procedures described in Example 26A and substituting HS- (CH ₂ -L-Leu-CH ₂ ) -OTBS with other L-configuration compounds of Formula 27 there are obtained the corresponding L-configuration compounds of Formula 28.

EXAMPLE 27 DETERMINATION OF FIBROBLAST COLLAGENASE (HFC) INHIBITION Starting Materials and Reagents:

Pro-HFC was purified from the harvest media of human gingival fibroblasts following procedures described in Birkedal-Hansen, H. Methods Enzymol . 1987, 144, 140- 171.

The HFC used in the assays was either zymogen that had undergone spontaneous activation, or zymogen that had been activated by treatment with 100 μg/mL of trypsin for 15 min at 23°C, followed by the addition of a 4-fold excess of soybean trypsin inhibitor.

Kinetic Measurements: Assays were performed in 50 mM Tricine, 0.2 M

NaCl, 10 mM CaCl ₂ , pH 7.5 containing 5% methanol once the substrate and inhibitor were diluted into it. The buffer was freed from adventitious metal ions by extraction with dithizone in carbon tetrachloride (Holmquist, B. Methods Enzymol . 1988, 158, 6-10) . Stock solutions of inhibitors were prepared in 100% methanol. Stock solutions of the substrate were prepared in 50% aqueous methanol at a concentration of 0.2 mM.

The assay method used was based on the hydrolysis of DNP-Pro-Leu-Ala-Leu-Trp-Ala-Arg at 24°C

(Netzel-Arnett, S.; Mallya, S. K. ; Nagase, H.; Birkedal- Hansen, H.; Van Wart, H. E. Anal. Biochem. 1991, 195, 86- 92) . The fluorescence changes were monitored with a Perkin-Elmer Model LS-5 fluorometer using an excitation wavelength of 280 nm and an emission wavelength of 360 nm. The substrate concentration used in the assays was either 5 μM or 10 μ-M. The inhibitor was diluted into the assays using 100% methanol, and controls substituted an equal volume of methanol so that the final methanol concentration from inhibitor and substrate dilutions in all assays was 5%. For each assay, the enzyme and

inhibitor were incubated in the assay buffer at 24°C for 30 min, then the substrate was added and the rate of hydrolysis was measured by monitoring the increase in fluorescence intensity at 360 nm. The inhibition results are expressed as the inhibitor concentration that produced 50% inhibition (IC ₅₀ ) of activity at the substrate concentration used.

Representative compounds of the present invention exhibited inhibition of HFC when tested by this method.

EXAMPLE 28 DETERMINATION OF NEUTROPHIL COLLAGENASE (HNC) INHIBITION Starting Materials and Reagents: HNC (58 kDa active form) was isolated from human buffy coats following procedures described in Mookhtiar, K. A.; Van Wart, H. E. Biochemistry 1990, 29, 10620-10627.

HNC was isolated in active form and no additional treatments were performed before use in assays .

Kinetic Measurements:

The preparation of enzyme, inhibitor and substrate solutions; and the assay method were performed using the procedure described in Example 21 (Kinetic Measurements Section) .

Representative compounds of the present invention exhibited inhibition of HNC when tested by this method.

EXAMPLE 29 DETERMINATION OF FIBROBLAST GELATINASE (HFG) INHIBITION Starting Materials and Reagents:

Pro-HFG was purified from the harvest media of human gingival fibroblasts following procedures described in Birkedal-Hansen, H. Methods Enzymol . 1987, 144, 140- 171.

Spontaneously activated HFG was used without additional treatment.

Kinetic Measurements:

The preparation of enzyme, inhibitor and substrate solutions; and the assay method were performed using the procedure described in Example 21 (Kinetic Measurements Section) .

Representative compounds of the present invention exhibited inhibition of HFG when tested by this method.

EXAMPLE 30

DETERMINATION OF NEUTROPHIL GELATINASE (HNG) INHIBITION Starting Materials and Reagents:

Pro-HNG was isolated from human buffy coats following procedures described in Mookhtiar, K. A.; Van Wart, H. E. Biochemistry 1990, 29, 10620-10627.

Spontaneously activated HNG was used without additional treatment.

Kinetic Measurements: The preparation of enzyme, inhibitor and substrate solutions; and the assay method were performed using the procedure described in Example 21 (Kinetic Measurements Section) .

Representative compounds of the present invention exhibited inhibition of HNG when tested by this method.

EXAMPLE 31 DETERMINATION OF STROMELYSIN (HFS) INHIBITION Starting Materials and Reagents:

Pro-HFS was isolated from the culture medium of human rheumatoid syi.ovial cells stimulated with rabbit macrophage-conditioned medium by affinity chromatography using sheep anti-HFS IgG coupled to Affi-Gel 10 following procedures described in Ito, A.; Nagase, H. Arch . Biochem. Biophys . 1988, 267, 211-216. Pro-HFS was activated by treatment with 1 mM p- aminophenylmercurie acetate (APMA) for 24 hr at 37°C to give a mixture of 45 and 28 kDa species, which are known to have indistinguishable specific activities and specificities (following procedures described in Okada, Y.; Nagase, H.; Harris, E. D. , Jr. J. Biol . Chem. 1986, 261, 14245-14255) . HFS was separated from the APMA by chromatography over Sephacryl S-200 and it was stored at 4°C.

Kinetic Measurements:

The preparation of enzyme, inhibitor and substrate solutions; and the assay method were performed using the procedure described in Example 21 (Kinetic Measurements Section) . Representative compounds of the present invention exhibited inhibition of HFS when tested by this method.

EXAMPLE 32 Capsule Formulation

This example illustrates the preparation of a representative pharmaceutical formulation for oral administration containing an active compound of Formula I, e.g., Me-R5-S0(NH) - (CH ₂ -L-Leu) -Phe-Ala-NH ₂ .

Ingredients Quantity (mg/capsule)

Active compound 200 lactose, spray-dried 148 magnesium stearate 2

The above ingredients are mixed and introduced into a hard-shell gelatin capsule.

Other compounds of Formula I, such as those prepared in accordance with Reaction Schemes A and B, and Examples 1-22 can be used as the active compound in the preparation of the orally administrable formulations of this example.

EXAMPLE 33 Oral Formulation

This example illustrates the preparation of a representative pharmaceutical formulation containing an active compound of Formula I, e.g., n-Bu-S (NH) ₂ - (CH ₂ -L>L- TyrOCH ₃ ) -Trp-NHBn. An suspension for oral administration is prepared having the following composition:

Ingredients Quantity

Active compound 1.0 g fumaric acid 0.5 g sodium chloride 2.0 g methyl paraben 0.1 g granulated sugar 25.5 g sorbitol (70% solution) 12.85 g

Veegum K (Vanderbilt Co. ) 1.0 g flavoring 0.035 mL colorings 0.5 mg distilled water q.s . to 100 mL

Other compounds of Formula I, such as those prepared in accordance with Reaction Schemes A and B, and Examples 1-22 can be used as the active compound in the

preparation of the orally administrable formulations of this example.

EXAMPLE 34 Tablet Formulation

This example illustrates the preparation of a representative pharmaceutical formulation containing an active compound of Formula I, e.g., n-Bu-S (NH) ₂ - (CH ₂ -DL- Leu) -Trp-NHBn. A tablet for oral administration is prepared having the following composition:

Ingredients Quantity (mg/tablet)

Active compound 400 corn starch 50 lactose 145 magnesium stearate 5

The above ingredients are mixed intimately and pressed into single scored tablets.

Other compounds of Formula I, such as those prepared in accordance with Reaction Schemes A and B, and Examples 1-22 can be used as the active compound in the preparation of the tablet formulations of this example.

EXAMPLE 35 Injectable Formulation

This example illustrates the preparation of a representative pharmaceutical formulation containing an active compound of Formula I, e.g., Me-f.5-S0(NH) - (CH ₂ -L- Leu) -Phe-Ala-NH ₂ .

An injectable preparation is prepared having the following composition:

Ingredients Quantity

Active compound 0.2 g water (distilled, sterile) q.s. to 20.0 mL

Other compounds of Formula I, such as those prepared in accordance with Reaction Schemes A and B, and Examples 1-20 can be used as the active compound in the preparation of the injection administrable formulations of this example.

EXAMPLE 36 Suppository Formulation

This example illustrates the preparation of a representative pharmaceutical formulation containing an active compound of Formula I, e.g., n-Bu-S (NH) ₂ - (CH ₂ -DL- Leu) -Trp-NHBn.

A suppository totalling 2.5 grams is prepared having the following composition:

Ingredients Quantity

Active compound 500 mg witepsol H-15 ^* q.s. to 2.5 g

("triglycerides of saturated vegatable fatty acid; a product of Riches-Nelson, Inc., New York, N.Y.) . Other compounds of Formula I, such as those prepared in accordance with Reaction Schemes A and B, and Examples 1-22 can be used as the active compound in the preparation of the suppository formulations of this example.

While the present invention has been described with reference to the specific embodiments thereof, it should be understood by those skilled in the art that various changes may be made and equivalents may be substituted without departing from the true spirit and scope of the invention. In addition, many modifications

may be made to adapt a particular situation, material, composition of matter, process, process step or steps, to the objective, spirit and scope of the present invention. All such modifications are intended to be within the scope of the claims appended hereto.