Title:
SUPRAMOLECULAR PROTEIN ASSEMBLY PREPARATION METHOD
Document Type and Number:
WIPO Patent Application WO/2016/143989
Kind Code:
A1
Abstract:
The present invention relates to a supramolecular assembly preparation method and a method for displaying a protein on a supramolecular assembly, the supramolecular assembly preparation method comprising the steps of: (a) preparing a plurality of assembly monomers comprising (i) a first split fragment containing green fluorescent protein (GFP) β-strand 1-10 and (ii) a second split fragment containing GFP β-strand 11 coupled to the terminus of the first split fragment; and (b) preparing a supramolecular assembly by carrying out self-assembly of a plurality of the assembly monomers. In addition, the present invention relates to a method for purifying a supramolecular assembly of a polydisperse distribution into a monodisperse supramolecular assembly, comprising the steps of: (a) mutating assembly monomers such that the negative charge value increases by reducing the net charge of the assembly monomers; (b) preparing a negatively charged supramolecular assembly of a polydisperse distribution by carrying out a plurality of self-assemblies of the assembly monomers; (c) separating the supramolecular assembly of a polydisperse distribution according to the size of the assembly; and (d) collecting the separated monodisperse supramolecular assembly.
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Inventors:
JUNG YONG WON (KR)
KIM YOUNG EUN (KR)
KIM YOUNG EUN (KR)
Application Number:
PCT/KR2015/013668
Publication Date:
September 15, 2016
Filing Date:
December 14, 2015
Export Citation:
Assignee:
KOREA ADVANCED INST SCI & TECH (KR)
International Classes:
C07K19/00; C07K1/00; C07K1/107; C07K1/14; G01N33/533
Foreign References:
| US20050221343A1 | 2005-10-06 | |||
| US20070161067A1 | 2007-07-12 |
Other References:
STEPANENKO ET AL.: "Beta-barrel Scaffold of Fluorescent Proteins: Folding, Stability and Role in Chromophore Formation", INTERNATIONAL REVIEW OF CELL AND MOLECULAR BIOLOGY, vol. 302, 2013, pages 221 - 278, XP055309379
KEEM ET AL.: "Splitting and Self-assembling of Far-red Fluorescent Protein with an Engineered Beta Strand Peptide: Application for Alpha-synuclein Imaging in Mammalian Cells", BIOMATERIALS, vol. 32, no. 34, 2011, pages 9051 - 9058, XP028300022
CABANTOUS ET AL.: "A New Protein-protein Interaction Sensor based on Tripartite Split-GFP Association", SCIENTIFIC REPORTS, vol. 3, no. 2854, 4 October 2013 (2013-10-04), pages 1 - 9, XP055260864
KEEM ET AL.: "Splitting and Self-assembling of Far-red Fluorescent Protein with an Engineered Beta Strand Peptide: Application for Alpha-synuclein Imaging in Mammalian Cells", BIOMATERIALS, vol. 32, no. 34, 2011, pages 9051 - 9058, XP028300022
CABANTOUS ET AL.: "A New Protein-protein Interaction Sensor based on Tripartite Split-GFP Association", SCIENTIFIC REPORTS, vol. 3, no. 2854, 4 October 2013 (2013-10-04), pages 1 - 9, XP055260864
Attorney, Agent or Firm:
YANG, Boo Hyun (KR)
양부현 (KR)
양부현 (KR)
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