Title:
SYNTHESIS OF POLYNUCLEOTIDES HAVING RANDOM SEQUENCES
Document Type and Number:
WIPO Patent Application WO1999021873
Kind Code:
A3
Abstract:
Subject of this invention is a process for the chemical synthesis of polynucleotides having totally or partially random sequences, based on the utilization, as synthesis monomer units for the random sequence part, or presynthetized mononucleotides and dinucleotides. Said synthesis is carried out on separate supports, so that on each of those supports is alternated at least one reaction cycle wherein a mixture of said dinucleotides is bound, with at least one reaction cycle wherein a mononucleotide is bound, and that in a preferred embodiment at the end of the n cycles required for a codon synthesis, the supports are mixed and then redivided into one or more reaction containers. The resulting polynucleotides are such that, for the random sequence part, each trinucleotide unit is fit to match only a limited number of codons, predefined for each unity in number and sequence, and the genetic code degeneracy effects can thus be eliminated. Figure 1 shows the chemical structure of the dinucleotides utilized as monomer units for the synthesis of codons forming the final sequence.
Inventors:
NEUNER PHILIPPE (IT)
Application Number:
PCT/IT1998/000298
Publication Date:
September 16, 1999
Filing Date:
October 28, 1998
Export Citation:
Assignee:
ANGELETTI P IST RICHERCHE BIO (IT)
NEUNER PHILIPPE (IT)
NEUNER PHILIPPE (IT)
International Classes:
C07H1/00; C12N15/09; C07H21/00; C07H21/02; C07H21/04; (IPC1-7): C07H21/00
Domestic Patent References:
| WO1983002626A1 | 1983-08-04 |
Foreign References:
| US5629179A | 1997-05-13 | |||
| US5523388A | 1996-06-04 |
Other References:
A.L.KAYUSHIN ET AL.: "A Convenient Approach to the Synthesis of Trinucleotide Phosphoramidites-Synthons for the Generation of Oligonucleotide / Peptide Libraries.", NUCLEIC ACIDS RESEARCH, vol. 24, no. 19, 1996, pages 3748 - 3755, XP002107801
S.F.ALTSCHUL ET AL.: "Significance of Nucleotide Sequence Alignments : a Method for Random Sequence Permutation that Preserves Dinucleotide and Codon Usage.", MOL. BIOL. EVOL., vol. 2, no. 6, 1985, pages 526 - 538, XP002107802
S.F.ALTSCHUL ET AL.: "Significance of Nucleotide Sequence Alignments : a Method for Random Sequence Permutation that Preserves Dinucleotide and Codon Usage.", MOL. BIOL. EVOL., vol. 2, no. 6, 1985, pages 526 - 538, XP002107802
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