Title:
SYSTEM AND METHOD FOR INSTANTLY DETECTING NUCLEIC ACID OF PATHOGEN
Document Type and Number:
WIPO Patent Application WO/2022/174663
Kind Code:
A1
Abstract:
A system and a method for instantly detecting nucleic acid of a pathogen. The system comprises a lysis buffer, which is used for inactivating and lysing a sample to be detected, so as to release RNA; an RT pre-mixed solution, which is used for reversely transcribing the RNA in the sample into DNA; an RPA pre-mixed solution, which is used for performing an RPA reaction on the sample treated with the RT pre-mixed solution, so as to realize amplification of the DNA in the sample; a CRISPR reaction pre-mixed solution, which is used for performing a specific cleavage reaction on the sample treated with the RPA pre-mixed solution; and colloidal gold test paper, which is used for performing color development on the sample treated with the CRISPR reaction pre-mixed solution, to confirm whether the sample contains the nucleic acid of the pathogen. According to the above-mentioned design scheme, the RPA method, the CRISPR method and the colloidal gold method are coupled, so that the instant-detection system has the advantages of simplicity, sensitivity and specificity, instruments and equipment are not required, and same can be used to quickly obtain a result by means of simple operation at room temperature.
Inventors:
ZHAO WEI (CN)
LONG FEI (CN)
YANG XIAONAN (CN)
ZHAO GUOPING (CN)
LONG FEI (CN)
YANG XIAONAN (CN)
ZHAO GUOPING (CN)
Application Number:
PCT/CN2021/138141
Publication Date:
August 25, 2022
Filing Date:
December 15, 2021
Export Citation:
Assignee:
SHENZHEN INST OF ADV TECH CAS (CN)
International Classes:
C12Q1/6844; C12Q1/70; C12R1/93
Domestic Patent References:
| WO2020256553A1 | 2020-12-24 | |||
| WO2020245808A1 | 2020-12-10 |
Foreign References:
| CN113061650A | 2021-07-02 | |||
| CN110184329A | 2019-08-30 | |||
| CN111560482A | 2020-08-21 | |||
| CN112239795A | 2021-01-19 | |||
| CN111593141A | 2020-08-28 | |||
| CN111363860A | 2020-07-03 |
Other References:
ZHANG YONGJIANG: "One-Step Reverse Transcription Recombinase Polymerase Amplification (RT-RPA) Technology for detection of BPMV", JIANGSU AGRICULTURAL SCIENCES, JIANGSU ACADEMY OF AGRICULTURAL SCIENCES, CN, vol. 46, no. 21, 31 December 2018 (2018-12-31), CN , pages 96 - 98, XP055960065, ISSN: 1002-1302, DOI: 10.15889/j.issn.1002-1302.2018.21.023
Attorney, Agent or Firm:
BEIJING ZHONG XUN TONG DA INTELLECTUAL PROPERTY AGENCY CO., LTD. (CN)
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