Title:
THERMOTOLERANT PROTEIN GLUTAMINASE
Document Type and Number:
WIPO Patent Application WO/2022/107885
Kind Code:
A1
Abstract:
Disclosed is a protein glutaminase having improved thermotolerance. A protein glutaminase comprising a polypeptide indicated in any among (1) to (3) has improved thermotolerance. (1) A polypeptide comprising a SEQ ID NO: 1 or 2 amino acid sequence; (2) a polypeptide wherein one or more amino acid residues are substituted, added, inserted or lost in the amino acid sequence indicated by SEQ ID NO: 1 or 2 and exhibiting the same thermotolerance as the amino acid sequence indicated by SEQ ID NO: 1 or 2: and (3) a polypeptide having at least 76% sequence identity with the amino acid sequence indicated by SEQ ID NO: 1 or 2 and having similar thermotolerance to a polypeptide comprising the amino acid sequence indicated by SEQ ID NO: 1 or 2.
Inventors:
MATSUOKA TOMOHIRO (JP)
YACHI HIROYUKI (JP)
SENDA DAIKI (JP)
YUKI KENSUKE (JP)
YACHI HIROYUKI (JP)
SENDA DAIKI (JP)
YUKI KENSUKE (JP)
Application Number:
PCT/JP2021/042625
Publication Date:
May 27, 2022
Filing Date:
November 19, 2021
Export Citation:
Assignee:
AMANO ENZYME INC (JP)
International Classes:
C12N5/10; C12N1/15; C12N1/19; C12N1/21; C12N9/14; C12N15/31; C12N15/55; C12N15/63
Domestic Patent References:
| WO2010029685A1 | 2010-03-18 | |||
| WO2009113628A1 | 2009-09-17 | |||
| WO2012060409A1 | 2012-05-10 |
Foreign References:
| JP2000050887A | 2000-02-22 | |||
| JP2013504323A | 2013-02-07 |
Other References:
DATABASE UniProtKB/TrEMBL NCBI; 18 July 2018 (2018-07-18), ANONYMOUS : "RecName: Full=Gln_deamidase_2 domain-containing protein {ECO:0000259|Pfam:PF18626};", XP055934034, Database accession no. A0A2S9CPY3
DATABASE UniProtKB/TrEMBL NCBI; 26 February 2020 (2020-02-26), ANONYMOUS : "RecName: Full=Gln_deamidase_2 domain-containing protein {ECO:0000259|Pfam:PF18626};", XP055934035, Database accession no. A0A5D8ZJA4
DATABASE UniProtKB/TrEMBL NCBI; 11 December 2019 (2019-12-11), ANONYMOUS : "RecName: Full=Gln_deamidase_2 domain-containing protein {ECO:0000259|Pfam:PF18626}", XP055934039, Database accession no. A0A4Q1EZS4
TATIANA A. TATSUSOVATHOMAS L. MADDEN, FEMS MICROBIOL. LETT., vol. 174, 1999, pages 247 - 250
"Molecular Cloning, A Laboratory Manual", 1989, COLD SPRING HARBOR LABORATORY PRESS
SANGER ET AL., PROC. NATL. ACAD. SCI. USA, vol. 74, 1977, pages 5463
DATABASE UniProtKB/TrEMBL NCBI; 26 February 2020 (2020-02-26), ANONYMOUS : "RecName: Full=Gln_deamidase_2 domain-containing protein {ECO:0000259|Pfam:PF18626};", XP055934035, Database accession no. A0A5D8ZJA4
DATABASE UniProtKB/TrEMBL NCBI; 11 December 2019 (2019-12-11), ANONYMOUS : "RecName: Full=Gln_deamidase_2 domain-containing protein {ECO:0000259|Pfam:PF18626}", XP055934039, Database accession no. A0A4Q1EZS4
TATIANA A. TATSUSOVATHOMAS L. MADDEN, FEMS MICROBIOL. LETT., vol. 174, 1999, pages 247 - 250
"Molecular Cloning, A Laboratory Manual", 1989, COLD SPRING HARBOR LABORATORY PRESS
SANGER ET AL., PROC. NATL. ACAD. SCI. USA, vol. 74, 1977, pages 5463
Attorney, Agent or Firm:
TANAKA, Junya et al. (JP)
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