Title:
THROMBIN DERIVATIVES AND PROCESS FOR PRODUCING THE SAME, ANHYDROTHROMBIN DERIVATIVES AND PROCESS FOR PRODUCING THE SAME, PLATELET AGGLUTINATION-INDUCING COMPOSITIONS, METHOD OF INDUCING PLATELET AGGLUTINATION, CLINICAL TEST REAGENTS, CLINICAL TEST METHOD, THROMBOSIS INHIBITORS, ADSORBENT AND PROCESS FOR PRODUCING THE SAME,
Document Type and Number:
WIPO Patent Application WO/2002/077031
Kind Code:
A1
Abstract:
Thrombin derivatives having a lowered fibrinogen activity and anhydrothrombin derivatives having a lowered fibrinogen activity are provided. Namely, thrombin derivatives wherein carboxyl of thrombin is modified; and anhydrothrombin derivatives wherein carboxyl of anhydrothrombin is modified. Because of having a lowered affinity for fibrinogen, these thrombin derivatives are usable as platelet agglutination-inducing compositions and specific coagulation reaction reagents for clinical tests, etc. By affinity chromatography with the use of anhydrothrombin as a ligand bonded to carboxyl, blood coagulation factor VIII can be more selectively purified. The anhydrothrombin derivatives having modified carboxyl exert a sufficient antithrombotic effect in a smaller amount than conventional anhydrothrombin does, which makes them usable as thrombosis inhibitors.
Inventors:
HOSOKAWA KAZUYA (JP)
Application Number:
PCT/JP2002/002594
Publication Date:
October 03, 2002
Filing Date:
March 19, 2002
Export Citation:
Assignee:
FUJIMORI KOGYO CO (JP)
CHISSO CORP (JP)
HOSOKAWA KAZUYA (JP)
CHISSO CORP (JP)
HOSOKAWA KAZUYA (JP)
International Classes:
A61K38/48; A61P7/02; A61P43/00; C07K14/745; C12N9/74; G01N33/86; (IPC1-7): C07K14/745; G01N33/86; C07K1/22; A61K38/36; A61K38/48; A61P7/02; A61P43/00
Foreign References:
| EP0882789A2 | 1998-12-09 | |||
| EP0611774A1 | 1994-08-24 | |||
| EP0615978A1 | 1994-09-21 | |||
| JPH1149800A | 1999-02-23 |
Other References:
V. W. F. CHAN: "Inactivation of Bovine Thrombin by water-soluble Carbodiimides: The essential Carboxyl Group has a pKa of 5.51", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 151, no. 2, 15 March 1998 (1998-03-15)
T. TOMONO: "Preparation of Anhydro-thrombin and its interaction with Plasma Antithrombin III", ACTA HAEMATOL. JPN., vol. 49, 1986, pages 969 - 979
K. HOSOKAWA: "Preparation of anhydrothrombin and Characterization of its interaction with natural thrombin substrates", BIOCHEM. J., vol. 354, 2001, pages 309 - 313
T. TOMONO: "Preparation of Anhydro-thrombin and its interaction with Plasma Antithrombin III", ACTA HAEMATOL. JPN., vol. 49, 1986, pages 969 - 979
K. HOSOKAWA: "Preparation of anhydrothrombin and Characterization of its interaction with natural thrombin substrates", BIOCHEM. J., vol. 354, 2001, pages 309 - 313
Attorney, Agent or Firm:
Hatta, Mikio (11-9 Nibancho Chiyoda-ku, Tokyo, JP)
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