Title:
TOXOFLAVIN HAPTEN, ARTIFICIAL ANTIGEN AND ANTIBODY, SYNTHESIS METHODS THEREFOR AND APPLICATIONS THEREOF
Document Type and Number:
WIPO Patent Application WO/2020/248316
Kind Code:
A1
Abstract:
Disclosed are a toxoflavin hapten, artificial antigen and antibody, synthesis methods therefor and applications thereof. An aliphatic chain connecting arm is derived on a nitrogen atom at position 1 of toxoflavin, and the terminal of the derived aliphatic chain connecting arm is provided with carboxyl. The toxoflavin hapten maximally retains a feature structure of the toxoflavin, and an antigenic determinant of the hapten can be exposed better, so that the immunogenicity of the toxoflavin hapten is obviously enhanced, and the toxoflavin hapten has the carboxyl which can be coupled with a carrier protein. The toxoflavin hapten is coupled with the carrier protein to obtain a toxoflavin immunizing antigen for immunizing an animal, which is more beneficial for stimulating immune response of the animal to generate an antibody having stronger specificity and higher sensitivity. An immunological method using the antibody can detect 0.02 ng/ml toxoflavin contamination, and provides a simple, convenient and rapid solution for bongkrek acid intoxication. The whole detection process only requires over ten minutes, and actual detection requirements can be completely met.
Inventors:
KE YUEBIN (CN)
LI JINFENG (CN)
LI CHENGYAO (CN)
DING YE (CN)
ZHAO FANG (CN)
LIU BOCHAO (CN)
LI JINFENG (CN)
LI CHENGYAO (CN)
DING YE (CN)
ZHAO FANG (CN)
LIU BOCHAO (CN)
Application Number:
PCT/CN2019/095182
Publication Date:
December 17, 2020
Filing Date:
July 09, 2019
Export Citation:
Assignee:
SHENZHEN CENTER FOR DISEASE CONTROL AND PREVENTION SHENZHEN HEALTH INSPECTION CENTER SHENZHEN INST O (CN)
International Classes:
C07D487/04; C07K14/765; C07K14/77; C07K14/79; C07K16/44; G01N33/577
Foreign References:
| CN110294762A | 2019-10-01 | |||
| CN105319313A | 2016-02-10 | |||
| CN106018659A | 2016-10-12 | |||
| CN105974018A | 2016-09-28 | |||
| CN109824604A | 2019-05-31 |
Other References:
WENG, CHENHUI ET AL.: "Advances in the Synthesis, Separation and Detection Technology of Toxoflavin", CHINESE BULLETIN OF LIFE SCIENCES, vol. 29, no. 4, 30 April 2017 (2017-04-30), DOI: 20200215110247
LI, RONGQING ET AL.: "Progress in the Synthesis of Artificial Antigen", CHINA BIOTECHNOLOGY, vol. 38, no. 12, 31 December 2018 (2018-12-31), DOI: 20200215110422
LI, RONGQING ET AL.: "Progress in the Synthesis of Artificial Antigen", CHINA BIOTECHNOLOGY, vol. 38, no. 12, 31 December 2018 (2018-12-31), DOI: 20200215110422
Attorney, Agent or Firm:
YOGO PATENT & TRADE MARK AGENCY LIMITED COMPANY (CN)
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