Title:
TRANSGENIC PLANT HAVING REDUCED HEAVY METAL CONTENT, AND METHOD FOR PRODUCING SAME
Document Type and Number:
WIPO Patent Application WO/2022/055144
Kind Code:
A1
Abstract:
The present invention relates to: plant cells in which the expression or activity of a phytochelatin synthase (PCS) gene or a protein encoded by the PCS gene is reduced compared to parent cells; a plant including the plant cells and having a reduced amount of absorbed heavy metals; a method for reducing the heavy metal content in a plant, the method including a step for reducing the expression or activity of a PCS gene or a protein encoded by the PCS gene compared to that in parent cells of the plant; a CRISPR-Cas9 recombinant vector including a single guide RNA targeting a PCS gene; and a method for producing a plant having a reduced heavy metal content, the method including a step for transforming plant cells by means of the recombinant vector.
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Inventors:
SEO HYO SEOK (KR)
LEE YOUNG GI (KR)
LEE JEONG HEON (KR)
KIM KWANG CHUL (KR)
NA WOONG HYUN (KR)
OH KYOUNG HWAN (KR)
JEON EUN YOUNG (KR)
LEE YOUNG GI (KR)
LEE JEONG HEON (KR)
KIM KWANG CHUL (KR)
NA WOONG HYUN (KR)
OH KYOUNG HWAN (KR)
JEON EUN YOUNG (KR)
Application Number:
PCT/KR2021/011059
Publication Date:
March 17, 2022
Filing Date:
August 19, 2021
Export Citation:
Assignee:
KT & G CORP (KR)
International Classes:
A01H1/06; A01H6/82; C12N9/10; C12N9/22; C12N15/10; C12N15/82
Foreign References:
| US10370675B2 | 2019-08-06 |
Other References:
LARSSON E HELENE, HAKAN ASP, BORNMAN JANET F: "Influence of prior Cd 2q exposure on the uptake of Cd 2q and other elements in the phytochelatin-deficient mutant, cad1-3, of Arabidopsis thaliana", JOURNAL OF EXPERIMENTAL BOTANY, vol. 53, no. 368, 1 March 2002 (2002-03-01), pages 447 - 453, XP055911016, DOI: 10.1093/jexbot/53.368.447
RAI PRABHAT KUMAR; KIM KI-HYUN; LEE SANG SOO; LEE JIN-HONG: "Molecular mechanisms in phytoremediation of environmental contaminants and prospects of engineered transgenic plants/microbes", SCIENCE OF THE TOTAL ENVIRONMENT, ELSEVIER, AMSTERDAM, NL, vol. 705, 6 December 2019 (2019-12-06), AMSTERDAM, NL , XP085994701, ISSN: 0048-9697, DOI: 10.1016/j.scitotenv.2019.135858
CLEMENS, S.: "Toxic metal accumulation, responses to exposure and mechanisms of tolerance in plants", BIOCHIMIE, MASSON, PARIS, FR, vol. 88, no. 11, 1 November 2006 (2006-11-01), FR , pages 1707 - 1719, XP027919521, ISSN: 0300-9084
KAERENLAMPI S , ET AL: "Genetic engineering in the improvement of plants for phytoremediation of metal polluted soils", ENVIRONMENTAL POLLUTION, BARKING, GB, vol. 107, no. 2, 1 January 2000 (2000-01-01), GB , pages 225 - 231, XP002287818, ISSN: 0269-7491, DOI: 10.1016/S0269-7491(99)00141-4
SEO, H.S: "Mutations of phytochelatin synthase (PCS) genes by CRISPR/Cas9 reduce accumulation of cadmium in tobacco leaves", 13 October 2019 (2019-10-13), pages 1, XP009535738, Retrieved from the Internet
RAI PRABHAT KUMAR; KIM KI-HYUN; LEE SANG SOO; LEE JIN-HONG: "Molecular mechanisms in phytoremediation of environmental contaminants and prospects of engineered transgenic plants/microbes", SCIENCE OF THE TOTAL ENVIRONMENT, ELSEVIER, AMSTERDAM, NL, vol. 705, 6 December 2019 (2019-12-06), AMSTERDAM, NL , XP085994701, ISSN: 0048-9697, DOI: 10.1016/j.scitotenv.2019.135858
CLEMENS, S.: "Toxic metal accumulation, responses to exposure and mechanisms of tolerance in plants", BIOCHIMIE, MASSON, PARIS, FR, vol. 88, no. 11, 1 November 2006 (2006-11-01), FR , pages 1707 - 1719, XP027919521, ISSN: 0300-9084
KAERENLAMPI S , ET AL: "Genetic engineering in the improvement of plants for phytoremediation of metal polluted soils", ENVIRONMENTAL POLLUTION, BARKING, GB, vol. 107, no. 2, 1 January 2000 (2000-01-01), GB , pages 225 - 231, XP002287818, ISSN: 0269-7491, DOI: 10.1016/S0269-7491(99)00141-4
SEO, H.S: "Mutations of phytochelatin synthase (PCS) genes by CRISPR/Cas9 reduce accumulation of cadmium in tobacco leaves", 13 October 2019 (2019-10-13), pages 1, XP009535738, Retrieved from the Internet
Attorney, Agent or Firm:
Y.P.LEE, MOCK & PARTNERS (KR)
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