TECHNICAL FIELD

The present invention relates to the immobilization of the mannanase enzyme partially purified from Lactobacillus invictae on zinc oxide (ZnO) nanoparticles and its use in facilitating tomato seed germination (average root and shoot length).

STATE OF THE ART

During germination, development and degradation of seeds, enzymes play an active role. P- mannanase is one of the main enzymes responsible for the hydrolysis of mannans during germination of tomato seeds through hydrolytic degradation of cell walls and weakening of endosperm structures surrounding the embryo and ensuring root formation. Since they metabolize the mannan in the cell wall, they are very important in improving plant metabolism as well as maturation.

DESCRIPTION OF THE INVENTION

The said invention relates to the immobilization of the mannanase enzyme partially purified from Lactobacillus invictae on zinc oxide (ZnO) nanoparticles and its use in facilitating tomato seed germination (average root and shoot length).

Beta (P)-mannanase is the main enzyme group that disrupts mannan structures in plant tissues. They are very important in growth and development because they metabolize mannan in the cell wall. The immobilized (ZnO) mannanase enzyme, which is environmentally friendly, harmless, sustainable, effective, reliable, of biological origin, does not have any negative effect on the environment, human and animals, which disrupts the mannan structure in plants and transforms it into a form that facilitates the germination of seeds, will have a positive effect on the germination of tomato seed due to the advantages and advantages it will provide. BRIEF DESCRIPTION OF THE FIGURES

Figure 1. a: FTIR image of immobilized mannanase enzyme in the ZnO nanoparticle, b: FTIR image of free mannanase enzyme, c: FTIR image of ZnO nanoparticle Figure 2. ZnO-NPs and Enzyme + ZnO-NPs XRD spectra

Figure 3. a: ZnO-NPs SEM image (10-2 pm), b: Enzyme + ZnONPs SEM image (20-10 pm)

BRIEF DESCRIPTION OF THE INVENTION

The present invention is the production of mannanase enzyme immobilized on the ZnO nanoparticle, characterized in that it comprises the following process steps:

A. Partial purification of the mannanase enzyme

B. Immobilization of mannanase on ZnO nanoparticles

C. Determination of the biochemical properties of the enzyme

D. Characterization of immobilized mannanase.

Elements related to the intelligibility of the present invention are as follows:

Mannanase: The enzyme group that breaks down P-1,4 mannose bonds in galactomannans. ZnO Nanoparticle: Nanoscale ZnO

Seed Germination: The development of the embryo in the seed under favorable conditions and its release from the seed to produce a plant that resembles the main plant.

The said invention is the use of mannanase enzyme immobilized on ZnO nanoparticle, characterized in that it is used in the soil as facilitator of tomato seed germination.