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Title:
USE OF AGRIMONIA PILOSA EXTRACT IN PREPARATION OF DRUG FOR REMOVING PLANT VIRUS IN TOMATO PLANTS
Document Type and Number:
WIPO Patent Application WO/2018/214641
Kind Code:
A1
Abstract:
Disclosed are the use of an Agrimonia pilosa extract in the preparation of a drug for removing a plant virus in tomato plants, and a method for removing the plant virus in the tomato plants using the Agrimonia pilosa extract. Aimed at the problem that currently there is no effective way in production to remove the virus in plants so as to achieve the treatment of viral diseases, the treatment of tomato plants with the Agrimonia pilosa extract can remove the tomato yellow leaf curl virus in tomato plants, which is beneficial to the treatment of viral diseases in plants. The Agrimonia pilosa extract is prepared by the following method: pulverizing traditional Chinese medicine, i.e. Agrimonia pilosa, with a universal pulverizer, weighing 200 g of dry powder into a 1000 mL triangular flask, adding 500 mL of 75% ethanol, leaching for 14 days at room temperature, filtering, concentrating under reduced pressure in a water bath using a rotary evaporator, evaporating the alcohol, obtaining the traditional Chinese medicine extract and drying same, and then dissolving same in 25 mL of acetone; the original concentration of the Agrimonia pilosa extract being expressed by the following formula: concentration = the dry weight of the medicinal material/final volume, and calculating according to same, the original concentration of Agrimonia pilosa being 8g/mL, and storing the extract in a refrigerator at 4°C for standby application, thus obtaining the Agrimonia pilosa extract.

Inventors:
GUO HUIFANG (CN)
ZHANG KAILI (CN)
ZHANG ZHICHUN (CN)
ZHAO DONGXIAO (CN)
Application Number:
PCT/CN2018/080974
Publication Date:
November 29, 2018
Filing Date:
March 28, 2018
Export Citation:
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Assignee:
JIANGSU ACAD AGRICULTURAL SCI (CN)
International Classes:
A01N63/04; A01N65/34; A01P7/04
Foreign References:
CN107212029A2017-09-29
CN102630710A2012-08-15
CN104430331A2015-03-25
CN104996231A2015-10-28
KR20160029969A2016-03-16
CN200810156163A2008-10-06
CN200810156162A2008-10-06
Other References:
QU, YUFENG: "Characteristics of symbiont and tomato yellow leaf curl virus infection in Bemisia tabaci, and effects of extract from Agrimonia pilosa on natural enemies", MASTER'S DISSERTATION, 30 June 2012 (2012-06-30), pages 1 - 84, XP009515745
See also references of EP 3545765A4
Attorney, Agent or Firm:
JIANGSU SUNDY LAW FIRM (CN)
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