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Title:
USE OF MMBBK2 IN PREPARATION OF TRYPSIN AND CHYMOTRYPSIN INHIBITORS
Document Type and Number:
WIPO Patent Application WO/2024/008205
Kind Code:
A1
Abstract:
The present invention belongs to the technical fields of genetic engineering and enzyme engineering, and specifically relates to the use of MmBBK2 in the preparation of trypsin and chymotrypsin inhibitors. The MmBBK2 has an amino acid sequence as shown in SEQ ID NO. 1 or SEQ ID NO. 2. In the present invention, it is confirmed that MmBBK2 in mulberry leaves has inhibitory activities on both trypsin and chymotrypsin for the first time, and the physical and chemical properties thereof are disclosed. The MmBBK2 has good application prospects in the preparation of trypsin and chymotrypsin inhibitors. On the basis that the physical and chemical properties of MmBBK2 are determined, the activities of the MmBBK2 can be eliminated in a targeted manner, so that the development and utilization of mulberry leaves in animal feeds are promoted, new perspectives and ideas are provided for the development and utilization of mulberry leaves in animal feeds and health foods, and the economic benefits of mulberry resources are improved.

Inventors:
LI YOUSHAN (CN)
ZHU RUI (CN)
LUO ZHUXING (CN)
Application Number:
PCT/CN2023/111382
Publication Date:
January 11, 2024
Filing Date:
August 07, 2023
Export Citation:
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Assignee:
UNIV SHAANXI TECHNOLOGY (CN)
International Classes:
C12N9/50; C07K14/81; C12N1/21; C12N15/57; C12N15/70; C12R1/19
Foreign References:
CN115725551B2023-05-05
CN113785933A2021-12-14
CN102584987A2012-07-18
Attorney, Agent or Firm:
XI'AN MINGZE INTELLECTUAL PROPERTY AGENCY OFFICE (GENERAL PARTNERSHIP) (CN)
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