Title:
VECTOR EXPRESSING RECOMBINANT ANTIGEN BY USING CRISPR EDITING TECHNOLOGY, AND SIMULTANEOUS MULTIPLE INSERTION METHOD THEREFOR
Document Type and Number:
WIPO Patent Application WO/2020/055163
Kind Code:
A1
Abstract:
Provided in the present invention is a method for simultaneously inserting multiple vectors expressing recombinant antigens in multiple locations of eukaryotic chromosomes by using a homology directed recombination method, which is a CRISPR technology. The present invention relates to a method for expressing a recombinant protein which can be used as an immunogen, by selecting a human cell line that can stably over-express an immunogen, when simultaneous insertion into two sites is performed, and then using the cells, and which can be effectively used for the development of recombinant immunogens and vaccines against various socially problematic viruses.
Inventors:
KIM CHONSAENG (KR)
KIM SEONG JUN (KR)
AHN DAE GYUN (KR)
KIM KYUN DO (KR)
KU KEUNBON (KR)
KIM HAE SOO (KR)
SHIN HYE JIN (KR)
HWANG IN SU (KR)
YOON GUN YOUNG (KR)
CHANG HYUN JOO (KR)
HUYNH THI NGOC DUYEN (KR)
KIM MIHWA (KR)
KIM BUM TAE (KR)
BAE SANGSU (KR)
WOO JAESUNG (KR)
KIM SEONG JUN (KR)
AHN DAE GYUN (KR)
KIM KYUN DO (KR)
KU KEUNBON (KR)
KIM HAE SOO (KR)
SHIN HYE JIN (KR)
HWANG IN SU (KR)
YOON GUN YOUNG (KR)
CHANG HYUN JOO (KR)
HUYNH THI NGOC DUYEN (KR)
KIM MIHWA (KR)
KIM BUM TAE (KR)
BAE SANGSU (KR)
WOO JAESUNG (KR)
Application Number:
PCT/KR2019/011830
Publication Date:
March 19, 2020
Filing Date:
September 11, 2019
Export Citation:
Assignee:
KOREA RES INST CHEMICAL TECH (KR)
KOREA FOOD RES INST (KR)
IUCF HYU (KR)
UNIV KOREA RES & BUS FOUND (KR)
KOREA FOOD RES INST (KR)
IUCF HYU (KR)
UNIV KOREA RES & BUS FOUND (KR)
International Classes:
C12N15/67; C07K14/005; C12N9/22; C12N15/10; C12N15/113; C12N15/90
Foreign References:
| US20160298137A1 | 2016-10-13 |
Other References:
MA CUIQING ET AL: "Searching for an ideal vaccine candidate among different MERS coronavirus receptor-binding fragments-the importance of immunofocusing in subunit vaccine design", VACCINE, vol. 32, no. 46, 19 September 2014 (2014-09-19), pages 6170 - 6176, XP029049168, DOI: 10.1016/j.vaccine.2014.08.086
RONDA, C.: "CrEdit: CRISPR mediated multi-loci gene integration in Saccharomyces cerevisiae", MICROBIAL CELL FACTORIES, vol. 14, no. 1, 7 July 2015 (2015-07-07), pages 97, XP021226447
BRESSAN, R. B.: "Efficient CRISPR/Cas9-assisted gene targeting enables rapid and precise genetic manipulation of mammalian neural stem cells", DEVELOPMENT, vol. 144, no. 4, 17 January 2017 (2017-01-17), pages 635 - 648, XP055602219
CHENG, J. K.: "Identifying and retargeting transcriptional hot spots in the human genome", BIOTECHNOLOGY JOURNAL, vol. 11, no. 8, August 2016 (2016-08-01), pages 1100 - 1109, XP055694234
TAI, W.: "A recombinant receptor-binding domain of MERS-CoV in trimeric form protects human dipeptidyl peptidase 4 (hDPP4) transgenic mice from MERS-CoV infection", VIROLOGY, vol. 499, December 2016 (2016-12-01), pages 375 - 382, XP055694235
RONDA, C.: "CrEdit: CRISPR mediated multi-loci gene integration in Saccharomyces cerevisiae", MICROBIAL CELL FACTORIES, vol. 14, no. 1, 7 July 2015 (2015-07-07), pages 97, XP021226447
BRESSAN, R. B.: "Efficient CRISPR/Cas9-assisted gene targeting enables rapid and precise genetic manipulation of mammalian neural stem cells", DEVELOPMENT, vol. 144, no. 4, 17 January 2017 (2017-01-17), pages 635 - 648, XP055602219
CHENG, J. K.: "Identifying and retargeting transcriptional hot spots in the human genome", BIOTECHNOLOGY JOURNAL, vol. 11, no. 8, August 2016 (2016-08-01), pages 1100 - 1109, XP055694234
TAI, W.: "A recombinant receptor-binding domain of MERS-CoV in trimeric form protects human dipeptidyl peptidase 4 (hDPP4) transgenic mice from MERS-CoV infection", VIROLOGY, vol. 499, December 2016 (2016-12-01), pages 375 - 382, XP055694235
Attorney, Agent or Firm:
B&IP-JOOWON PATENT AND LAW FIRM (KR)
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