Title:
デジタルＰＣＲ計測装置
Document Type and Number:
Japanese Patent JP7361864
Kind Code:
B2
Abstract:
A digital PCR measuring apparatus capable of measuring a melting curve with high accuracy is provided. The digital PCR measuring apparatus includes a temperature adjuster that controls a temperature of a sample container including a plurality of minute regions, a fluorescence measurement part that measures fluorescence intensity of a plurality of minute regions, and a controller that controls the temperature adjuster and the fluorescence measurement part. The controller controls the temperature adjuster to raise a temperature of the sample container, and, after removing air bubbles generated in the sample container, measures fluorescence intensity of a plurality of minute regions while controlling the temperature adjuster to lower the temperature of the sample container, and measures a melting curve of a plurality of the minute regions.
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Inventors:
Kiyo Nakagawa
Junko Tanaka
Yuzuru Shimazaki
Kunio Harada
Junko Tanaka
Yuzuru Shimazaki
Kunio Harada
Application Number:
JP2022167287A
Publication Date:
October 16, 2023
Filing Date:
October 19, 2022
Export Citation:
Assignee:
株式会社日立製作所
International Classes:
C12Q1/686; C12Q1/6813
Domestic Patent References:
| JP2012516455A | ||||
| JP2009535066A | ||||
| JP2010286421A | ||||
| JP2011083286A | ||||
| JP2017123813A | ||||
| JP2016024137A |
Foreign References:
| WO2018128013A1 | ||||
| WO2008096563A1 | ||||
| US20050123924 | ||||
| WO2002025289A1 | ||||
| WO2017138484A1 |
Other References:
Tanaka, J. et al.,"KRAS genotyping by digital PCR combined with melting curve analysis",Sci. Rep.,2019年02月22日,Vol. 9; 2626,pp. 1-8
Attorney, Agent or Firm:
Patent Attorney Corporation Hiraki International Patent Office