Title:
【発明の名称】組換えDNA発現ベクタ-およびペニシリウム・クリソゲナムのイソペニシリンNシンセタ-ゼをコ-ドしているDNA化合物
Document Type and Number:
Japanese Patent JPH0773499
Kind Code:
B2
Abstract:
The present invention comprises novel DNA compounds that encode isopenicillin N synthetase. The invention also comprises methods, transformants, and polypeptides related to the novel DNA compounds. The novel isopenicillin N synthetase-encoding DNA, together with its associated transcription and translation activating sequence, was isolated from Penicilliumchrysogenum. The isopenicillin N synthetase-encoding DNA can be used to construct novel E. coli expression vectors that drive expression of isopenicillin N synthetase in E. coli. The intact P. chrysogenum isopenicillin N synthetase-encoding DNA and associated transcription and translation activating sequence can also be used to construct expression vectors that drive expression of the isopenicillin N synthetase in P. chrysogenum and Cephalosporiumacremonium. The transcription and translation activating sequence can be fused to a hygromycin phosphotransferase-encoding DNA segment and placed onto expression vectors that function in P. chrysogenum and C. acremonium. The transcription termination and mRNA polyadenylation signals of the P. chrysogenum isopenicillin N synthetase can be used to increase ultimate expression of a product encoded on a recombinant DNA vector.
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Inventors:
Lucinda Gale Curl
Thomas Dominique Ingoria
Stephen Wyatt Queener
Paul Luther Skaterad
Thomas Dominique Ingoria
Stephen Wyatt Queener
Paul Luther Skaterad
Application Number:
JP28161386A
Publication Date:
August 09, 1995
Filing Date:
November 25, 1986
Export Citation:
Assignee:
Lee-Lee Lili-and Company-
International Classes:
C12N1/14; C12N1/15; C12N1/20; C12N1/21; C12N9/00; C12N15/00; C12N15/52; C12N15/69; C12N15/70; C12N15/80; C12N15/90; C12P37/00; C12N15/09; C12R1/19; C12R1/75; C12R1/82; (IPC1-7): C12N15/09
Attorney, Agent or Firm:
Aoyama Ryo (1 person outside)