Title:
【発明の名称】L―フェニルアセチルカルビノール(PAC)の製造方法、その方法に使用するための微生物及びその微生物を調製するための方法
Document Type and Number:
Japanese Patent JPH04501060
Kind Code:
A
Abstract:
Mutations are induced in a microorganism selected from the species Saccharomyces cerevisiae or from the species Candida flareri. The resulting mutants are cultured in the presence of a fermentation inhibitor, such as acetaldehyde, ephedrine or PAC-dione, to form colonies having resistance to the inhibitor. Cells from the colonies are isolated and tested for yield of phenyl acetyl carbinol (PAC) in a fermentation with benzaldehyde and pyruvate. Yeast cells from the colonies that produce elevated levels of PAC are selected for use in subsequent fermentations. PAC is useful as an intermediate in the preparation of l-ephedrine and d-pseudoephedrine, two well-known medicinal chemicals.
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Inventors:
Sealy, Robert Jay.
Hazeman, Robert Buoy.
Jarus, Michael Jay.
Sullivan, Sally A.
Hazeman, Robert Buoy.
Jarus, Michael Jay.
Sullivan, Sally A.
Application Number:
JP51092089A
Publication Date:
February 27, 1992
Filing Date:
October 13, 1989
Export Citation:
Assignee:
Signagen, Inc.
International Classes:
C12N1/16; C12P7/26; C12P13/00; C12R1/72; C12R1/865; (IPC1-7): C12N1/16; C12P7/26; C12P13/00
Attorney, Agent or Firm:
Aoki Akira (3 outside)