Title:
IMMUNODEFICIENT MOUSE
Document Type and Number:
WIPO Patent Application WO/2020/067199
Kind Code:
A1
Abstract:
The present invention addresses the problem of providing an immunodeficient mouse that is capable of excluding the influence of immunodeficient mouse-derived immune cells on human antibodies and has high engraftment of human cells. When a mouse FcgR gene is additionally deleted from a NOG mouse, the mouse does not express antibody-dependent cytotoxicity activity to tumors, and human cells are engrafted at a significantly higher rate compared to a NOG mouse. Furthermore, by engrafting human NK cells to the mouse that is introduced with a human IL-15 gene, only the human NK cells become effector cells, and ADCC activity can be evaluated.
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Inventors:
TAKAHASHI TAKESHI (JP)
KATANO IKUMI (JP)
KATANO IKUMI (JP)
Application Number:
PCT/JP2019/037675
Publication Date:
April 02, 2020
Filing Date:
September 25, 2019
Export Citation:
Assignee:
CENTRAL INST FOR EXPERIMENTAL ANIMALS (JP)
International Classes:
A01K67/027; C12N5/10; C12Q1/06; G01N33/48; C12N15/09
Domestic Patent References:
| WO2016189799A1 | 2016-12-01 | |||
| WO2017127414A1 | 2017-07-27 |
Foreign References:
| JP2004154058A | 2004-06-03 | |||
| JP2017536121A | 2017-12-07 | |||
| JP3753321B2 | 2006-03-08 | |||
| JP2016220559A | 2016-12-28 |
Other References:
GIRARD-PIERCE, K. R. ET AL.: "A novel role for C3 in antibody-induced red blood cell clearance and antigen modulation", BLOOD, vol. 122, no. 10, September 2013 (2013-09-01), pages 1793 - 1801, XP055700578
KATANO , I. ET AL.: "Long-term maintenance of peripheral blood derived human NK cells in a novel human IL -15- transgenic NOG mouse", SCIENTIFIC REPORTS, vol. 7, 8 December 2017 (2017-12-08), pages 17230, XP055700580
KATANO, I. ET AL.: "NOG-FcgR KO hIL-15 Tg mice provide a highly sensitive assay system for ADCC activity of human NK cells", CANCER RESEARCH, vol. 79, no. 13, July 2019 (2019-07-01), pages LB-032, XP009518900
OHBO K ET AL., BLOOD, 1996
M. F. W. FESTING: "Inbred Strains in Biomedical Research", 1979, THE MACMILLAN PRESS
KATANO , I. ET AL.: "Long-term maintenance of peripheral blood derived human NK cells in a novel human IL -15- transgenic NOG mouse", SCIENTIFIC REPORTS, vol. 7, 8 December 2017 (2017-12-08), pages 17230, XP055700580
KATANO, I. ET AL.: "NOG-FcgR KO hIL-15 Tg mice provide a highly sensitive assay system for ADCC activity of human NK cells", CANCER RESEARCH, vol. 79, no. 13, July 2019 (2019-07-01), pages LB-032, XP009518900
OHBO K ET AL., BLOOD, 1996
M. F. W. FESTING: "Inbred Strains in Biomedical Research", 1979, THE MACMILLAN PRESS
Attorney, Agent or Firm:
HIROTA, Masanori (JP)
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