METHOD FOR AMPLIFYING RNA, AND ITS USE IN EXPRESSION PROFILING

Title:

METHOD FOR AMPLIFYING RNA, AND ITS USE IN EXPRESSION PROFILING

Document Type and Number:

WIPO Patent Application WO2003106680

Kind Code:

B1

Abstract:

The invention relates to the field of enzymatic gene amplification, and particularly to the provision of multigene expression profiles derived from messenger RNA. Then invention provides a method for amplifying RNA from a sample, preferably for obtaining an expression profile of cellular RNA, comprising the step of amplifying (cellular) RNA by contacting said RNA with an a first amplification primer, an RNA-dependent polymerase and nucleotides to synthesize a copy nucleic acid from said RNA, wherein said first amplification primer comprises a 3'-fragment capable of hybridizing with a poly-A tail of mRNA and said RNA-dependent polymerase is capable of providing an extension to said first strand copy nucleic acid allowing hybridisation with a second amplification primer.

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Inventors:

BODDEKE ERIK HENDRIKUS WILHELM (NL)
BIBER KNUT PETER HEINRICH (NL)

Application Number:

PCT/NL2003/000433

Publication Date:

February 26, 2004

Filing Date:

June 13, 2003

Export Citation:

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Assignee:

UNIV GRONINGEN (NL)
BODDEKE ERIK HENDRIKUS WILHELM (NL)
BIBER KNUT PETER HEINRICH (NL)

International Classes:

C12P19/34; C12Q1/68; (IPC1-7): C12N15/10; C12Q1/68

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