Title:
METHOD FOR PREPARING CORE FUCOSE-CONTAINING ANTIBODY
Document Type and Number:
WIPO Patent Application WO/2017/018474
Kind Code:
A1
Abstract:
The present invention pertains to a method for preparing an antibody in which the content of sugar chains having core fucose is increased, from an antibody in which sugar chains having core fucose and sugar chains not having core fucose are mixed, the method characterized by performing at least the following steps (1) and (2): (1) a step of treating the antibody with endoglycosidase (1) so as to hydrolyze sugar chains bound to "N-acetylglucosamine to which fucose is not bound"; and (2) a step of removing the "antibodies in which the sugar chain bound to the N-acetylglucosamine to which fucose is not bound was hydrolyzed" obtained in step (1).
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Inventors:
TSUKIMURA WATARU (JP)
KUROGOCHI MASAKI (JP)
SHIRAI TAKASHI (JP)
TAKEGAWA KAORU (JP)
KINOSHITA TAKASHI (JP)
KUROGOCHI MASAKI (JP)
SHIRAI TAKASHI (JP)
TAKEGAWA KAORU (JP)
KINOSHITA TAKASHI (JP)
Application Number:
PCT/JP2016/072110
Publication Date:
February 02, 2017
Filing Date:
July 28, 2016
Export Citation:
Assignee:
THE NOGUCHI INST (JP)
International Classes:
C07K1/14; C07K1/16; C07K16/00; C07K16/28; C07K16/46; C12P21/08; C07K17/00; C12N5/10; C12N9/24
Foreign References:
| JP2015507925A | 2015-03-16 | |||
| JP2009531289A | 2009-09-03 | |||
| US20120226024A1 | 2012-09-06 |
Other References:
WATARU TSUKIMURA: "Kin'itsu Tosa o Motsu Trastuzumab (6) Core Fucose Gan'yu Tosa o Motsu IgG no Chosei", DAI 34 KAI THE JAPANESE SOCIETY OF CARBOHYDRATE RESEARCH NENKAI, 1 July 2015 (2015-07-01), pages 229
KUROGOCHI M. ET AL.: "Glycoengineered Monoclonal Antibodies with Homogeneous Glycan (M3, G0, G2, and A2) Using a Chemoenzymatic Approach Have Different Affinities for FcyRIIIa and Variable Antibody-Dependent Cellular Cytotoxicity Activities.", PLOS ONE, vol. 10, no. 7, 22 July 2015 (2015-07-22), pages 1 - 24, XP055239650, Retrieved from the Internet [retrieved on 20161018]
WATARU TSUKIMURA ET AL.: "Core Fucose Gan'yu Tosa o Motsu Tosa Kaihen Trastuzumab no Chosei to sono Kassei", DAI 88 KAI ANNUAL MEETING OF THE JAPANESE BIOCHEMICAL SOCIETY KOEN YOSHISHU, December 2015 (2015-12-01)
SHIRAI T. ET AL.: "Glycoengineered monoclonal antibodies using a chemoenzymatic approach and their affinities for FcyRIIIa andvariable antibody-dependent cellular cytotoxicities.", P ROGRAM AND ABSTRACTS FOR 2015 ANNUAL MEETING OF THE SOCIETY FOR GLYCOBIOLOGY, December 2015 (2015-12-01), pages 1273 - 1274
KUROGOCHI M. ET AL.: "Glycoengineered Monoclonal Antibodies with Homogeneous Glycan (M3, G0, G2, and A2) Using a Chemoenzymatic Approach Have Different Affinities for FcyRIIIa and Variable Antibody-Dependent Cellular Cytotoxicity Activities.", PLOS ONE, vol. 10, no. 7, 22 July 2015 (2015-07-22), pages 1 - 24, XP055239650, Retrieved from the Internet
WATARU TSUKIMURA ET AL.: "Core Fucose Gan'yu Tosa o Motsu Tosa Kaihen Trastuzumab no Chosei to sono Kassei", DAI 88 KAI ANNUAL MEETING OF THE JAPANESE BIOCHEMICAL SOCIETY KOEN YOSHISHU, December 2015 (2015-12-01)
SHIRAI T. ET AL.: "Glycoengineered monoclonal antibodies using a chemoenzymatic approach and their affinities for FcyRIIIa andvariable antibody-dependent cellular cytotoxicities.", P ROGRAM AND ABSTRACTS FOR 2015 ANNUAL MEETING OF THE SOCIETY FOR GLYCOBIOLOGY, December 2015 (2015-12-01), pages 1273 - 1274
Attorney, Agent or Firm:
TAKAHASHI INTERNATIONAL PATENT OFFICE (JP)
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