METHODS FOR DETECTION OF DIFFERENCES IN NUCLEIC ACIDS

Title:

METHODS FOR DETECTION OF DIFFERENCES IN NUCLEIC ACIDS

Document Type and Number:

WIPO Patent Application WO2001069200

Kind Code:

A3

Abstract:

The present invention provides methods for detecting the presence or absence of a difference between two related nucleic acid sequences. In the methods, a target nucleic acid and a reference nucleic acid are contacted under conditions in which they are capable of forming a four-way nucleic acid complex with a branch structure that is capable of migration. Under the contact conditions, if the reference nucleic acid and target nucleic acid are identical, branch migration is capable of going to completion resulting in complete strand exchange. If the reference nucleic acid and target nucleic acid are different, branch migration does not go to completion, resulting in a stable four-way complex. Detection of the stable four-way complex identifies the presence of a difference between the nucleic acids. A stable four-way complex can be detected with molecules that specifically bind such complexes, by gel electrophoresis or by specific isolation of the stable four-way complex.

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Inventors:

YANG QINGHONG
YANG WENDY
LISHANSKI ALLA

Application Number:

PCT/US2001/007858

Publication Date:

March 14, 2002

Filing Date:

March 12, 2001

Export Citation:

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Assignee:

FRESHGENE INC (US)

International Classes:

G01N33/53; C12N15/09; C12Q1/34; C12Q1/68; G01N33/566; (IPC1-7): C12Q1/68

Domestic Patent References:

WO1997023646A1

1997-07-03

Foreign References:

US6013439A	2000-01-11
US5824471A	1998-10-20
US5698400A	1997-12-16

Other References: