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Title:
PHOSPHOKINASE ASSAY
Document Type and Number:
WIPO Patent Application WO2004081534
Kind Code:
A3
Abstract:
A method of detecting protein kinases in a sample by coating a solid surface with a buffer containing protease and phosphatase inhibitors and immobilizing a labeled antibody to the coated solid surface, whereby protein kinases react with the antibody, thereby detecting a presence of the protein kinases. Also provided is an assay and kit for detecting protein kinases having antibodies specific to a phosphorylation site of the protein kinase and a label bound to the antibodies.

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Inventors:
SUPERNAULT JON M (US)
CAMERON MARK (US)
BRIEN SARA (US)
Application Number:
PCT/US2004/007569
Publication Date:
January 27, 2005
Filing Date:
March 12, 2004
Export Citation:
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Assignee:
ASSAY DESIGNS INC (US)
SUPERNAULT JON M (US)
CAMERON MARK (US)
BRIEN SARA (US)
International Classes:
C12N15/09; C12Q1/48; G01N33/53; G01N33/542; G01N33/573; G01N; (IPC1-7): C12N15/09; C12Q1/48; G01N33/53; G01N33/542
Foreign References:
JP2001161398A2001-06-19
US6406869B12002-06-18
US4594319A1986-06-10
US6670144B12003-12-30
US6630311B22003-10-07
Other References:
See also references of EP 1606404A4
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