Title:
WHOLE CELL BIOCATALYST
Document Type and Number:
WIPO Patent Application WO/2011/057820
Kind Code:
A3
Abstract:
The present invention relates to a method for producing a product of a reaction catalysed by a nitrilase, which method comprises the steps (i) providing a microorganism comprising said nitrilase located on its surface, and/or a membrane preparation of said microorganism, and (ii) contacting the microorganism and/or the membrane preparation thereof with one or more nitrilase substrates under conditions compatible with nitrilase activity. The present invention further relates to a method for producing enantiomerically pure (R)-mandelic acid using the nitrilase-displaying whole cell biocatalyst or membrane preparation thereof for the conversion of racemic mandelonitrile.
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Inventors:
JOSE JOACHIM (DE)
MAAS RUTH (DE)
DETZEL CHRISTIAN (DE)
MAAS RUTH (DE)
DETZEL CHRISTIAN (DE)
Application Number:
PCT/EP2010/006951
Publication Date:
July 07, 2011
Filing Date:
November 16, 2010
Export Citation:
Assignee:
ZYRUS BETEILIGUNGSGMBH & CO PATENTE I KG (DE)
JOSE JOACHIM (DE)
MAAS RUTH (DE)
DETZEL CHRISTIAN (DE)
JOSE JOACHIM (DE)
MAAS RUTH (DE)
DETZEL CHRISTIAN (DE)
International Classes:
C12N9/78; C12P7/42
Other References:
BANERJEE ANIRBAN ET AL: "Enantioselective Nitrilase from Pseudomonas putida: Cloning, Heterologous Expression, and Bioreactor Studies", MOLECULAR BIOTECHNOLOGY, vol. 41, no. 1, January 2009 (2009-01-01), pages 35 - 41, XP002577397, ISSN: 1073-6085
RENU SINGH ET AL: "Release of an enantioselective nitrilase from Alcaligenes faecalis MTCC 126: a comparative study", BIOPROCESS AND BIOSYSTEMS ENGINEERING, SPRINGER, BERLIN, DE LNKD- DOI:10.1007/S00449-005-0013-4, vol. 27, no. 6, 1 October 2005 (2005-10-01), pages 415 - 424, XP019347360, ISSN: 1615-7605
CHEN R R: "Permeability issues in whole-cell bioprocesses and cellular membrane engineering", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 200703 DE LNKD- DOI:10.1007/S00253-006-0811-X, vol. 74, no. 4, March 2007 (2007-03-01), pages 730 - 738, XP002577398, ISSN: 0175-7598
JOSE JOACHIM ET AL: "The autodisplay story, from discovery to biotechnical and biomedical applications", MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, vol. 71, no. 4, December 2007 (2007-12-01), pages 600 - 619, XP002577399, ISSN: 1092-2172
CHRISTIAN DETZEL, RUTH MAAS AND JOACHIM JOSE: "Autodisplay of Nitrilase from Alcaligenes faecalis in E coli Yields a Whole Cell Biocatalyst for the Synthesis of Enantiomerically Pure (R)-Mandelic Acid", CHEMCATCHEM, no. 3, 4 February 2011 (2011-02-04), pages 719 - 725, XP002636113, DOI: 10.1002/cctc.201000382
RENU SINGH ET AL: "Release of an enantioselective nitrilase from Alcaligenes faecalis MTCC 126: a comparative study", BIOPROCESS AND BIOSYSTEMS ENGINEERING, SPRINGER, BERLIN, DE LNKD- DOI:10.1007/S00449-005-0013-4, vol. 27, no. 6, 1 October 2005 (2005-10-01), pages 415 - 424, XP019347360, ISSN: 1615-7605
CHEN R R: "Permeability issues in whole-cell bioprocesses and cellular membrane engineering", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 200703 DE LNKD- DOI:10.1007/S00253-006-0811-X, vol. 74, no. 4, March 2007 (2007-03-01), pages 730 - 738, XP002577398, ISSN: 0175-7598
JOSE JOACHIM ET AL: "The autodisplay story, from discovery to biotechnical and biomedical applications", MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, vol. 71, no. 4, December 2007 (2007-12-01), pages 600 - 619, XP002577399, ISSN: 1092-2172
CHRISTIAN DETZEL, RUTH MAAS AND JOACHIM JOSE: "Autodisplay of Nitrilase from Alcaligenes faecalis in E coli Yields a Whole Cell Biocatalyst for the Synthesis of Enantiomerically Pure (R)-Mandelic Acid", CHEMCATCHEM, no. 3, 4 February 2011 (2011-02-04), pages 719 - 725, XP002636113, DOI: 10.1002/cctc.201000382
Attorney, Agent or Firm:
MEISSNER, BOLTE & PARTNER (München, DE)
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