Title:
脱リン酸化試薬の品質を評価する方法及び標的核酸を検出する方法
Document Type and Number:
Japanese Patent JP7402428
Kind Code:
B2
Abstract:
An object of the present invention is to provide a method for evaluating a quality of a dephosphorylation reagent containing an alkaline phosphatase, and a method for detecting a target nucleic acid by using a dephosphorylation reagent that has been evaluated as having a high quality by the evaluation method, and in order to achieve the object, the present invention provides a method for evaluating a quality of a dephosphorylation reagent, the method including the following steps of:(1-1) providing a dephosphorylation reagent containing an alkaline phosphatase and a peptide fragment derived from the alkaline phosphatase; and(1-2) evaluating the dephosphorylation reagent as having a high quality if a content ratio of the peptide fragment to the alkaline phosphatase is a predetermined reference value or less.
Inventors:
Masateru Ito
Yoji Ueda
Yuki Takii
Mai Yagi
Yoji Ueda
Yuki Takii
Mai Yagi
Application Number:
JP2019554582A
Publication Date:
December 21, 2023
Filing Date:
September 25, 2019
Export Citation:
Assignee:
Toray Industries, Inc.
International Classes:
C12Q1/42; C12Q1/68; G01N30/72; G01N30/88; G01N33/50
Foreign References:
| US20080098491 | ||||
| US20070148140 |
Other References:
POZIDIS C and BOURIOTIS V,Preparation of high purity alkaline phosphatase from calf intestine using dye-ligand chromatography,Bioseparation,vol.5,no.2,1995年,p.89-93
Attorney, Agent or Firm:
Hiroyuki Nagai
Yukitaka Nakamura
Satoru Asakura
Mari Asano
Hiroshi Sorimachi
Keiyasu Suzuki
Yukitaka Nakamura
Satoru Asakura
Mari Asano
Hiroshi Sorimachi
Keiyasu Suzuki