NUCLEIC ACID COMPRISING OR ENCODING HISTONE STEM LOOP AND POLY(A) SEQUENCE OR POLYADENYLATION SIGNAL FOR INCREASING EXPRESSION OF ENCODED PROTEIN

Title:

NUCLEIC ACID COMPRISING OR ENCODING HISTONE STEM LOOP AND POLY(A) SEQUENCE OR POLYADENYLATION SIGNAL FOR INCREASING EXPRESSION OF ENCODED PROTEIN

Document Type and Number:

Japanese Patent JP2016041080

Kind Code:

A

Abstract:

PROBLEM TO BE SOLVED: To provide a nucleic acid comprising or encoding a histone stem-loop and a poly(A) sequence or a polyadenylation signal for increasing the expression of an encoded protein.SOLUTION: This application describes a coding nucleic acid sequence, particularly a messenger RNA (mRNA), comprising or encoding for a histone stem-loop and a poly(A) sequence or a polyadenylation signal, and the use thereof for increasing the expression of an encoded protein. It also discloses its use for the preparation of a pharmaceutical composition, especially a vaccine, e.g., for the use in the treatment of tumors and cancer diseases, cardiovascular diseases, infectious diseases, autoimmune diseases or genetic diseases, or in gene therapy. The invention further describes an in vitro transcription method, in vitro methods for increasing the expression of a protein using a nucleic acid comprising or encoding for a histone stem-loop and a poly(A) sequence or a polyadenylation signal as well as an ex vivo and in vivo methods.SELECTED DRAWING: None

More Like This:

JP2000513930	INDUSTRIAL APPLICABILITY: A polynucleotide encoding a secretory protein from the human fetal brain.
JPH05199871	IMPROVED PRODUCTION METHOD OF GLYCOSYLTRANSFERASE
JP2004533231	Method for producing recombinant vector, transformed plant or a part thereof, transformed plant cell, transformed plant and transformed plant

Inventors:

ANDREAS THESS
THOMAS SCHLAKE
JOCHEN PROBST

Application Number:

JP2015247498A

Publication Date:

March 31, 2016

Filing Date:

December 18, 2015

Export Citation:

Click for automatic bibliography generation Help

Assignee:

CUREVAC GMBH

International Classes:

C12N15/09; A61K39/00; A61K39/002; A61K39/02; A61K39/12; A61K48/00; A61P1/00; A61P3/00; A61P3/02; A61P5/00; A61P9/00; A61P11/00; A61P17/00; A61P19/00; A61P21/00; A61P25/00; A61P27/02; A61P27/16; A61P31/00; A61P31/04; A61P31/10; A61P31/12; A61P33/00; A61P35/00; A61P37/02; A61P37/08; A61P43/00

Attorney, Agent or Firm:

Hidesaku Yamamoto
Natsuki Morishita
Takatoshi Iida
Daisuke Ishikawa
Kensaku Yamamoto

Previous Patent: RECOMBINANT AVIAN PARAMYXOVIRUS VACCINE AND METHOD FOR MAKING AND USING THE SAME

Next Patent: EPISOMAL REPROGRAMMING WITH CHEMICALS