Title:
原核および真核生物に関する核酸プローブに基づく診断アッセイ
Document Type and Number:
Japanese Patent JP5902749
Kind Code:
B2
Abstract:
Use of the ssrA gene or tmRNA, an RNA transcript of the ssrA gene, or fragments thereof as target regions in a nucleic acid probe assay for the detection and identification of prokaryotic and/or eukaryotic organisms is described. Nucleotide sequence alignment of tmRNA sequences from various organisms can be used to identify regions of homology and non-homology within the sequences which in turn can be used to design both genus specific and species specific oligonucleotide probes. These newly identified regions of homology and non-homology provide the basis of identifying and detecting organisms at the molecular level. Oligonucleotide probes identified in this way can be used to detect tmRNA in samples thereby giving an indication of the viability of non- viral organisms present in various sample types.
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Inventors:
Burleigh, Thomas Gerald
Smith, terence james
Smith, terence james
Application Number:
JP2014096195A
Publication Date:
April 13, 2016
Filing Date:
May 07, 2014
Export Citation:
Assignee:
Enterprise Ireland
National University of Ireland Galway
National University of Ireland Galway
International Classes:
C12N15/09; G01N33/50; A61K31/7088; A61K38/00; A61K48/00; A61P31/04; A61P33/00; C12Q1/68; C12Q1/689; G01N33/15; G01N33/53; G01N37/00
Domestic Patent References:
| JP3130099A | ||||
| JP4258299A | ||||
| JP6501385A |
Other References:
Nucleic Acids Res. ,1998年,vol.26, no.1,pp.163-165
RNA,1996年,vol.2, no.12,pp.1306-1310
RNA,1996年,vol.2, no.12,pp.1306-1310
Attorney, Agent or Firm:
Shinjiro Ono
Yasushi Kobayashi
Yasushi Kobayashi
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