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Title:
QUANTITATIVE ANALYSIS AND ITS KIT OF STRUCTURE PLASMINOGEN ACTIVATOR
Document Type and Number:
Japanese Patent JPS59174759
Kind Code:
A
Abstract:

PURPOSE: To effect a high-sensitive quantitative analysis of TPA by using a structure plasminogen activater (TPA) antibody 1gG fragment.

CONSTITUTION: To TPA is added NaH2PO4 buffer liquid added with salt, MgCl, serum alubumin, gelatin and sodiumaride and this liquid is added with a solid phase mode of combination of polyethylene ball with 1gG fragment separated and refined from a rabbit and added to a solution mode by dissoloving an enzym antibody prepared by reaction of β-D-galactosidase to the TPA antibody 1gG fragment in the buffer liquid and then the solid phase is separated and then activity of β-D-galactosidase is measured with a fluorescence photometer using 4-methylumbelliferyl-β-D-galactosido.


Inventors:
MATSUO OSAMU
Application Number:
JP4949883A
Publication Date:
October 03, 1984
Filing Date:
March 24, 1983
Export Citation:
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Assignee:
KOWA CO
MATSUO OSAMU
International Classes:
G01N33/53; A61K39/00; C12Q1/56; G01N33/86; (IPC1-7): A61K39/44; C12Q1/00; G01N33/54
Domestic Patent References:
JPS49124283A1974-11-28
JPS5344612A1978-04-21
Attorney, Agent or Firm:
Ariga Miyuki